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Additional file 2: Text S1. Expression profile of escape genes in fat and muscle. Text S2. Enrichment analysis of DEGs. Text S3. Correlation between DMPs and gene expression. Text S4. Correlation between DMRs and DEGs across tissues.

Supplemental material, sj-docx-1-jtt-10.1177_1357633X221150279 for Effect of telemonitoring on readmissions for acute exacerbation of chronic obstructive pulmonary disease: A randomized clinical trial by Frank D Andersen, Christian Trolle, Asger Roer Pedersen, Maria L Køpfli, Sanne Børgesen, Michael S Jensen, and Charlotte Hyldgaard in Journal of Telemedicine and Telecare

Additional file 1: Figure S1. Manhattan plots of X chromosomal differentially expressed genes. Figure S2. Venn diagrams of X chromosomal differentially expressed genes. Figure S3. Circular plots of PAR genes. Figure S4. Manhattan plots of autosomal differentially expressed genes. Figure S5. Densityplot of autosomal differentially expressed genes (aDEGs). Figure S6. Venn diagrams of autosomal differentially expressed genes. Figure S7. WGCNA analysis module 1–3. Figure S8. WGCNA analysis trait 1–3. Figure S9. WGCNA analysis module 4–6. Figure S10. WGCNA analysis trait 4–5. Figure S11. Venn diagrams of X chromosomal and autosomal differentially methylated sites. Figure S12. Venn diagram of X chromosomal and autosomal differentially methylated sites. Figure S13. Distribution of X chromosomal differentially methylated sites (xDMPs) and autosomal differentially methylated sites (aDMPs) between different contrasts in relation to genic region. Figure S14. Correlation plots of significant differentially methylated positions (DMPs) in correlation with gene expression. Figure S15. Correlation plots of significantly differentially methylated regions (DMRs) in correlation with gene expression.

Additional file 3: Figure F1. Boxplots illustrating differences in CPM (Count Per Million) at each differentially expressed gene with a crude p < 0.005 between type 1 diabetes exposed offspring and controls.

Additional file 5: Figure F2. Network plots depicting the genes that are involved in the significant GOBP terms from the Functional Enrichment Analysis of the gene expression changes observed in type 1 diabetes exposed offspring compared to matched controls.

Additional file 8: Figure F3. Weighted gene correlation network analysis (WGCNA) of all expressed genes. The genes were sorted into modules, with each module appointed a color, based on co-expression patters. The eigengene of each module was correlated to the measured clinical traits. The correlation values and p-values were depicted for each correlation, which were colored based on positive (red) or negative (blue) correlation.

With wider application and technical improvement of imaging the discovery of adrenal incidentalomas (AI) has been skyrocketing. AI need to be investigated for evidence of hormonal hypersecretion and malignancy, and this causes a considerable use of health resources and potential medicalisation. In the "no-need-to-see" process, the clinical assessment of the citizen will only take place, if the diagnostic tests are abnormal. In this review, we examine the predictive values of imaging and paraclinical testing and argue, that normal test results in people without extra-adrenal cancer exclude disease.

Background Pheochromocytomas and catecholamine-secreting paragangliomas (PPGL) are rare catecholamine-producing tumors. Due to the rarity, limited data on prognosis exists. Here, we present national data on mortality for radically operated patients compared to the background population over a period of 40 years. Materials and methods We have previously identified a national cohort of 588 PPGL patients diagnosed in Denmark 1977-2016. After excluding 80 patients diagnosed post-mortem, we found that out of 508 patients diagnosed alive, 479 (94%) underwent radical surgery. Each operated patient was matched on birth year, age and sex with 100 random controls from the general population. PPGL patients and comparison cohort members were then followed from date of surgery (or matched index date) to date of death, emigration or 31st of December 2016. Mortality rate-ratios (MRR) were calculated in patient-comparisons strata using Cox regression and adjusted for Charlson Comorbidity Index (CCI). CCI was determined using diagnosis codes registered before time of PPGL diagnosis (or matched index date). To identify prognostic factors, we obtained health records and analyzed clinical data in a geographic subgroup of patients (N=162). Results Operated PPGL patients had a median age of 55 years (Q1-Q3 42-65) and 56% were female. 26% of PPGL patients had a Charlson Comorbidity Index of 2 or more, compared to only 9% in the comparison cohort, with a higher proportion of patients registered with cardiovascular disease (19% vs 7%), cancer (16% vs 5%) and with diabetes (12% vs 3%). Median follow-up time for patients was 7.2 years (Q1-Q3 3.4-15.6), with 10- and 20-year survival after surgery of 78% (95% CI 73-82) and 64% (95% CI 57-69), respectively. MRR for PPGL patients was 1.8 (95% CI 1.5-2.2) compared to the comparison cohort and 1.4 (95% CI 1.2-1.7) when adjusted for CCI. In the subcohort of PPGL patients with available clinical data (N=162), we found, that patients diagnosed with PPGL due to diagnostic work-up for secondary hypertension (N=27) had the highest mortality after surgery with an adjusted MRR of 3.1 (95% CI 1.7-5.8) compared to these patients’ matched comparison cohorts. Adjusted MRR was 0.9 (95% CI 0.4-1.8, N=49) for patients evaluated for paroxysmal symptoms, 1.0 (95% CI 0.2-4.6, N=16) for patients diagnosed due to hereditary PPGL in family or after syndromic presentation, 1.3 (95% CI 0.7-2.2, N=58) for adrenal incidentalomas, and 2.2 (95% CI 0.9-4.9, N=10) in patients diagnosed due to other causes. Conclusion PPGL patients undergoing radical curative surgery have a higher mortality compared to the background population even when considering their higher comorbidity index at time of diagnosis. Especially patients diagnosed due to work-up for secondary hypertension have an increased mortality. These results indicate that radical surgery might not completely reverse the harmful effects of PPGL.

Table S1. Aortic distensibility in women with Turner Syndrome but no aortic coarctation compared to healthy age and gender matched controls. Overall model P-value

Pheochromocytoma is a rare disease but frequently poses a diagnostic dilemma due to the unspecific symptoms and its potentially life-threatening nature. There is a perception of an increase in the incidence of pheochromocytomas in recent years, but no data on time trends exist.We obtained data from The Danish National Registry of Patients, The Danish Registry of Causes of Death, and The National Pathology Registry for all persons registered with pheochromocytoma in 1977-2016. Health records were reviewed to validate the diagnosis for all patients in the Northern and Central Regions of Denmark (population 1.75 million). Incidence rates were calculated using Poisson regression. We identified 198 confirmed cases of pheochromocytoma. A significant increasing trend (pThe incidence of pheochromocytoma has increased significantly in recent years, presumably due to increased use of imaging studies. Incidentaloma patients appear to represent a new less symptomatic and older group of pheochromocytoma patients not previously diagnosed. Whether surgical treatment provides a health benefit in this group remains unknown.

BackgroundThough pheochromocytoma is a rare disease, it is a frequent diagnostic dilemma in adrenal incidentaloma individuals necessitating clarification due to the potential life-threatening nature of pheochromocytoma. There is a widespread perception of an increase in the number of patients with pheochromocytoma, but the true incidence, morbidity and mortality associated with pheochromocytoma remains to be fully elucidated.Materials and methodsThe cohort of potential cases of pheochromocytoma patients was identified with a data extract on relevant diagnoses from the National Patient Registry covering all years 1977-2014. In addition, data extracts from the National Pathology Registry, the Danish Registry of Causes of Death and the Danish Cancer Registry was performed to identify missing patients in the National Patient Registry.The pheochromocytoma diagnosis is currently being verified by a thorough study of the health records of all potential cases. When a diagnosis is confirmed or suspected, details - including age at diagnosis, symptoms at diagnosis, co-morbidity, location of pheochromocytoma, hereditary predisposition, operational technique, recurrence, etc. - is entered into a new database. These data will be used to describe the presentation of the disease in the clinic.Incidence rates of pheochromocytoma will be analyzed using Poisson regression, and confidence limits using an approximation to Poisson distribution.Prelimanary ResultsThe process of verifying pheochromocytoma is still ongoing with 107 cases of pheochromocyoma currently verified.

We present a case story of a patient suspected having Cushing's syndrome (CS), with spuriously elevated P-cortisol level diagnosed by overnight and two days dexamethasone suppression test (DST). The patient was being treated with carbamazepine, and after two weeks withdrawal from this drug, both two-day DST and urinary free cortisol showed normal results. There are many pitfalls in the process of diagnosing CS. Several conditions mimic the disease and others cause hypercortisolism. Liver and kidney failure as well as several drugs can influence the test results. It is important to bare this in mind during the diagnostic work-up of suspected CS.