91 results on '"Troia, R"'
Search Results
2. Comparison of clinicopathological patterns of renal tubular damage in dogs with acute kidney injury caused by leptospirosis and other aetiologies
- Author
-
Zamagni, S., Troìa, R., Zaccheroni, F., Monari, E., Grisetti, C., Perissinotto, L., Balboni, A., and Dondi, F.
- Published
- 2020
- Full Text
- View/download PDF
3. Prospective evaluation of rapid point-of-care tests for the diagnosis of acute leptospirosis in dogs
- Author
-
Troìa, R., Balboni, A., Zamagni, S., Frigo, S., Magna, L., Perissinotto, L., Battilani, M., and Dondi, F.
- Published
- 2018
- Full Text
- View/download PDF
4. Evaluation of the delta neutrophil index from an automated blood cell analyser in septic dogs
- Author
-
Troìa, R., Agnoli, C., Calipa, S., Segalina, S., Murgia, E., Gruarin, M., Dondi, F., and Giunti, M.
- Published
- 2017
- Full Text
- View/download PDF
5. Autologous transplant vs oral chemotherapy and lenalidomide in newly diagnosed young myeloma patients: a pooled analysis
- Author
-
Gay, F, Oliva, S, Petrucci, M T, Montefusco, V, Conticello, C, Musto, P, Catalano, L, Evangelista, A, Spada, S, Campbell, P, Ria, R, Salvini, M, Offidani, M, Carella, A M, Omedé, P, Liberati, A M, Troia, R, Cafro, A M, Malfitano, A, Falcone, A P, Caravita, T, Patriarca, F, Nagler, A, Spencer, A, Hajek, R, Palumbo, A, and Boccadoro, M
- Published
- 2017
- Full Text
- View/download PDF
6. Carfilzomib, Pomalidomide, and Dexamethasone As Second-line Therapy for Lenalidomide-refractory Multiple Myeloma
- Author
-
Sonneveld, P., Zweegman, S., Cavo, Michele, Nasserinejad, Kazem, Broijl, A., Troia, R., Croockewit, S., Minnema, Monique C., Boccadoro, Mario, Sonneveld, P., Zweegman, S., Cavo, Michele, Nasserinejad, Kazem, Broijl, A., Troia, R., Croockewit, S., Minnema, Monique C., and Boccadoro, Mario
- Abstract
Contains fulltext : 285916.pdf (Publisher’s version ) (Open Access)
- Published
- 2022
7. Carfilzomib, Pomalidomide, and Dexamethasone As Second-line Therapy for Lenalidomide-refractory Multiple Myeloma
- Author
-
Sonneveld, P, Zweegman, S, Cavo, M, Nasserinejad, K, Broijl, A, Troia, R, Pour, L, Croockewit, S, Corradini, P, Patriarca, F, Wu, KL, Droogendijk, J, Bos, G, Hajek, R, Petrucci, MT, Ypma, P, Zojer, N, Minnema, MC, Boccadoro, M, Sonneveld, P, Zweegman, S, Cavo, M, Nasserinejad, K, Broijl, A, Troia, R, Pour, L, Croockewit, S, Corradini, P, Patriarca, F, Wu, KL, Droogendijk, J, Bos, G, Hajek, R, Petrucci, MT, Ypma, P, Zojer, N, Minnema, MC, and Boccadoro, M
- Abstract
This phase 2 trial investigated reinduction with carfilzomib, pomalidomide, and dexamethasone (KPd) and continuous pomalidomide/dexamethasone in patients at first progression during lenalidomide maintenance. The second objective was to evaluate high-dose melphalan with autologous stem cell transplantation (HDM/ASCT) at first progression. Patients were eligible who had progressive disease according to International Myeloma Working Group (IMWG) criteria. Treatment consisted of 8 cycles carfilzomib (20/36 mg/m2), pomalidomide (4 mg) and dexamethasone. Patients without prior transplant received HDM/ASCT. Pomalidomide 4 mg w/o dexamethasone was given until progression. One hundred twelve patients were registered of whom 86 (77%) completed 8 cycles of KPd. Thirty-five (85%) eligible patients received HDM/ASCT. The median time to discontinuation of pomalidomide w/o dexamethasone was 17 months. Best response was 37% ≥ complete response, 75% ≥ very good partial response, 92% ≥ partial response, respectively. At a follow-up of 40 months median PFS was 26 and 32 months for patients who received KPd plus HDM/ASCT and 17 months for patients on KPd (hazard ratio [HR] 0.61, 95% confidence interval [CI] 0.37-1.00, P = 0.051). PFS was better after longer duration of prior lenalidomide (HR 3.56, 95% CI 1.42-8.96, P = 0.035). Median overall survival (OS) was 67 months. KPd-emerging grade 3 and 4 adverse events included hematologic (41%), cardiovascular (6%), respiratory (3%), infections (17%), and neuropathy (2%). KPd followed by continuous pomalidomide is an effective and safe triple drug regimen in second-line for patients previously exposed to bortezomib and/or refractory to lenalidomide.
- Published
- 2022
8. Management of two healthcare-associated infections outbreaks of Serratia marcescens and Enterobacter cloacae in a Veterinary University Hospital, and implementation of a surveillance system
- Author
-
Scarpellini R, Troia R, Giunti M, Mondo E, Giacometti F, Savini F, Tumietto F, Dondi F, Magagnoli I, Pisoni L, Cola V, Balboni A, Battilani M, Piva S, and Scarpellini R, Troia R, Giunti M, Mondo E, Giacometti F, Savini F, Tumietto F, Dondi F, Magagnoli I, Pisoni L, Cola V, Balboni A, Battilani M, Piva S
- Subjects
Healthcare-associated infections, Serratia marcescens, Enterobacter cloacae, Veterinary University Hospital, surveillance system - Published
- 2021
9. Septic shock in dogs: clinical features and prognostic significance
- Author
-
Ciuffoli E, Troia R, Mascalzoni G, Pianini V, Giunti M, and Ciuffoli E, Troia R, Mascalzoni G, Pianini V, Giunti M
- Subjects
shock, sepsis, cryptic, vasoplegic, dysoxic - Abstract
Introduction: septic shock is a subset of sepsis characterized by significantly increased mortality in people. A novel proposed classification of septic shock based on 3 different phenotypes (cryptic, hyperlactatemia without persistent hypotension; vasoplegic, persistent hypotension without hyperlactatemia; and dysoxic, persistent hyperlactatemia and hypotension) seems helpful for outcome prediction. Our aim was to report the clinical features and outcome of dogs with septic shock, including the prevalence of the 3 different septic shock phenotypes, compared to dogs with uncomplicated sepsis. Methods: Dogs with sepsis, identified as SIRS plus confirmation of infection, and hospitalized in the intensive care unit were prospectively included (June 2018 –December 2019). Presence of septic shock was defined by persistent hypotension requiring vasopressor therapy or persistent hyperlactatemia after adequate fluid resuscitation Dogs with septic shock were further classified as affected by cryptic, vasoplegic, or dysoxic shock according to the previously cited human criteria. The canine Acute Patient Physiological and Laboratory Evaluation (APPLEfast) score was calculated and the presence of multiorgan dysfunction syndrome (MODS), defined as previously reported for dogs, was evaluated upon admission in the whole population. Nonparametric statistical tests were performed, and significance set at P < 0.05. Results: One-hundred-thirty-one septic dogs were enrolled; 31/131 (24%) had septic shock: cryptic (10/31, 32%), dysoxic (18/31, 58%), and vasoplegic (3/31, 10%). Septic shock dogs had significantly higher APPLEfast Scores (28, 13-38 vs 25, 6-33, P = 0.0051) and plasma lactate (3.5, 1.3-9.9 vs 2.2, 0.4-7.1, P < 0. 001), and significantly greater frequencies of MODS (74.2% vs 7%, P
- Published
- 2020
10. Coinfection with canine circovirus and canine adenovirus type 1 and 2 in dogs affected by parvovirosis
- Author
-
Terrusi A, Urbani L, Stefanelli SAM, Troia R, Giunti M, Dondi F, Battilani M, Balboni A, and Terrusi A, Urbani L, Stefanelli SAM, Troia R, Giunti M, Dondi F, Battilani M, Balboni A
- Subjects
canine circoviru ,dog ,canine parvovirus ,canine adenoviru ,coinfection - Published
- 2020
11. Consolidation and Maintenance in Newly Diagnosed Multiple Myeloma
- Author
-
Sonneveld, P., Dimopoulos, M.A., Beksac, M., Holt, B. van der, Aquino, S., Ludwig, H., Zweegman, S., Zander, T., Zamagni, E., Wester, R., Hajek, R., Pantani, L., Dozza, L., Gay, F., Cafro, A., Rosa, L. De, Morelli, A., Gregersen, H., Gulbrandsen, N., Cornelisse, P., Troia, R., Oliva, S., Velden, V. van de, Wu, K., Ypma, P.F., Bos, G., Levin, M.D., Pour, L., Driessen, C., Broijl, A., Croockewit, A.J., Minnema, M.C., Waage, A., Hveding, C., Donk, N. van de, Offidani, M., Palumbo, G.A., Spencer, A., Boccadoro, M., Cavo, M., Sonneveld, P., Dimopoulos, M.A., Beksac, M., Holt, B. van der, Aquino, S., Ludwig, H., Zweegman, S., Zander, T., Zamagni, E., Wester, R., Hajek, R., Pantani, L., Dozza, L., Gay, F., Cafro, A., Rosa, L. De, Morelli, A., Gregersen, H., Gulbrandsen, N., Cornelisse, P., Troia, R., Oliva, S., Velden, V. van de, Wu, K., Ypma, P.F., Bos, G., Levin, M.D., Pour, L., Driessen, C., Broijl, A., Croockewit, A.J., Minnema, M.C., Waage, A., Hveding, C., Donk, N. van de, Offidani, M., Palumbo, G.A., Spencer, A., Boccadoro, M., and Cavo, M.
- Abstract
Item does not contain fulltext, PURPOSE: To address the role of consolidation treatment for newly diagnosed, transplant eligible patients with multiple myeloma in a controlled clinical trial. PATIENTS AND METHODS: The EMN02/HOVON95 trial compared consolidation treatment with two cycles of bortezomib, lenalidomide, and dexamethasone (VRD) or no consolidation after induction and intensification therapy, followed by continuous lenalidomide maintenance. Primary study end point was progression-free survival (PFS). RESULTS: Eight hundred seventy-eight eligible patients were randomly assigned to receive VRD consolidation (451 patients) or no consolidation (427 patients). At a median follow-up of 74.8 months, median PFS with adjustment for pretreatment was prolonged in patients randomly assigned to VRD consolidation (59.3 v 42.9 months, hazard ratio [HR] = 0.81; 95% CI, 0.68 to 0.96; P = .016). The PFS benefit was observed across most predefined subgroups, including revised International Staging System (ISS) stage, cytogenetics, and prior treatment. Revised ISS3 stage (HR, 2.00; 95% CI, 1.41 to 2.86) and ampl1q (HR, 1.67; 95% CI, 1.37 to 2.04) were significant adverse prognostic factors. The median duration of maintenance was 33 months (interquartile range 13-86 months). Response ≥ complete response (CR) after consolidation versus no consolidation before start of maintenance was 34% versus 18%, respectively (P < .001). Response ≥ CR on protocol including maintenance was 59% with consolidation and 46% without (P < .001). Minimal residual disease analysis by flow cytometry in a subgroup of 226 patients with CR or stringent complete response or very good partial response before start of maintenance demonstrated a 74% minimal residual disease-negativity rate in VRD-treated patients. Toxicity from VRD was acceptable and manageable. CONCLUSION: Consolidation treatment with VRD followed by lenalidomide maintenance improves PFS and depth of response in newly diagnosed patients with multiple myeloma as compared to
- Published
- 2021
12. Reversible myocardial dysfunction in a dog after resuscitation from cardiopulmonary arrest
- Author
-
Magagnoli, I., primary, Romito, G., additional, Troia, R., additional, Murgia, E., additional, and Giunti, M., additional
- Published
- 2021
- Full Text
- View/download PDF
13. Autologous haematopoietic stem-cell transplantation versus bortezomib–melphalan–prednisone, with or without bortezomib–lenalidomide–dexamethasone consolidation therapy, and lenalidomide maintenance for newly diagnosed multiple myeloma (EMN02/HO95): a multicentre, randomised, open-label, phase 3 study
- Author
-
Cavo, M. Gay, F. Beksac, M. Pantani, L. Petrucci, M.T. Dimopoulos, M.A. Dozza, L. van der Holt, B. Zweegman, S. Oliva, S. van der Velden, V.H.J. Zamagni, E. Palumbo, G.A. Patriarca, F. Montefusco, V. Galli, M. Maisnar, V. Gamberi, B. Hansson, M. Belotti, A. Pour, L. Ypma, P. Grasso, M. Croockewit, A. Ballanti, S. Offidani, M. Vincelli, I.D. Zambello, R. Liberati, A.M. Andersen, N.F. Broijl, A. Troia, R. Pascarella, A. Benevolo, G. Levin, M.-D. Bos, G. Ludwig, H. Aquino, S. Morelli, A.M. Wu, K.L. Boersma, R. Hajek, R. Durian, M. von dem Borne, P.A. Caravita di Toritto, T. Zander, T. Specchia, G. Waage, A. Gimsing, P. Mellqvist, U.-H. van Marwijk Kooy, M. Minnema, M. Mandigers, C. Cafro, A.M. Palmas, A. Carvalho, S. Spencer, A. Boccadoro, M. Sonneveld, P.
- Abstract
Background: The emergence of highly active novel agents has led some to question the role of autologous haematopoietic stem-cell transplantation (HSCT) and subsequent consolidation therapy in newly diagnosed multiple myeloma. We therefore compared autologous HSCT with bortezomib–melphalan–prednisone (VMP) as intensification therapy, and bortezomib–lenalidomide–dexamethasone (VRD) consolidation therapy with no consolidation. Methods: In this randomised, open-label, phase 3 study we recruited previously untreated patients with multiple myeloma at 172 academic and community practice centres of the European Myeloma Network. Eligible patients were aged 18–65 years, had symptomatic multiple myeloma stage 1–3 according to the International Staging System (ISS), measurable disease (serum M protein >10 g/L or urine M protein >200 mg in 24 h or abnormal free light chain [FLC] ratio with involved FLC >100 mg/L, or proven plasmacytoma by biopsy), and WHO performance status grade 0–2 (grade 3 was allowed if secondary to myeloma). Patients were first randomly assigned (1:1) to receive either four 42-day cycles of bortezomib (1·3 mg/m2 administered intravenously or subcutaneously on days 1, 4, 8, 11, 22, 25, 29, and 32) combined with melphalan (9 mg/m2 administered orally on days 1–4) and prednisone (60 mg/m2 administered orally on days 1–4) or autologous HSCT after high-dose melphalan (200 mg/m2), stratified by site and ISS disease stage. In centres with a double HSCT policy, the first randomisation (1:1:1) was to VMP or single or double HSCT. Afterwards, a second randomisation assigned patients to receive two 28-day cycles of consolidation therapy with bortezomib (1·3 mg/m2 either intravenously or subcutaneously on days 1, 4, 8, and 11), lenalidomide (25 mg orally on days 1–21), and dexamethasone (20 mg orally on days 1, 2, 4, 5, 8, 9, 11, and 12) or no consolidation; both groups received lenalidomide maintenance therapy (10 mg orally on days 1–21 of a 28-day cycle). The primary outcomes were progression-free survival from the first and second randomisations, analysed in the intention-to-treat population, which included all patients who underwent each randomisation. All patients who received at least one dose of study drugs were included in the safety analyses. This study is registered with the EU Clinical Trials Register (EudraCT 2009-017903-28) and ClinicalTrials.gov (NCT01208766), and has completed recruitment. Findings: Between Feb 25, 2011, and April 3, 2014, 1503 patients were enrolled. 1197 patients were eligible for the first randomisation, of whom 702 were assigned to autologous HSCT and 495 to VMP; 877 patients who were eligible for the first randomisation underwent the second randomisation to VRD consolidation (n=449) or no consolidation (n=428). The data cutoff date for the current analysis was Nov 26, 2018. At a median follow-up of 60·3 months (IQR 52·2–67·6), median progression-free survival was significantly improved with autologous HSCT compared with VMP (56·7 months [95% CI 49·3–64·5] vs 41·9 months [37·5–46·9]; hazard ratio [HR] 0·73, 0·62–0·85; p=0·0001). For the second randomisation, the number of events of progression or death at data cutoff was lower than that preplanned for the final analysis; therefore, the results from the second protocol-specified interim analysis, when 66% of events were reached, are reported (data cutoff Jan 18, 2018). At a median follow-up of 42·1 months (IQR 32·3–49·2), consolidation therapy with VRD significantly improved median progression-free survival compared with no consolidation (58·9 months [54·0–not estimable] vs 45·5 months [39·5–58·4]; HR 0·77, 0·63–0·95; p=0·014). The most common grade ≥3 adverse events in the autologous HSCT group compared to the VMP group included neutropenia (513 [79%] of 652 patients vs 137 [29%] of 472 patients), thrombocytopenia (541 [83%] vs 74 [16%]), gastrointestinal disorders (80 [12%] vs 25 [5%]), and infections (192 [30%] vs 18 [4%]). 239 (34%) of 702 patients in the autologous HSCT group and 135 (27%) of 495 in the VMP group had at least one serious adverse event. Infection was the most common serious adverse event in each of the treatment groups (206 [56%] of 368 and 70 [37%] of 189). 38 (12%) of 311 deaths from first randomisation were likely to be treatment related: 26 (68%) in the autologous HSCT group and 12 (32%) in the VMP group, most frequently due to infections (eight [21%]), cardiac events (six [16%]), and second primary malignancies (20 [53%]). Interpretation: This study supports the use of autologous HSCT as intensification therapy and the use of consolidation therapy in patients with newly diagnosed multiple myeloma, even in the era of novel agents. The role of high-dose chemotherapy needs to be reassessed in future studies, in particular in patients with undetectable minimal residual disease after four-drug induction regimens including a monoclonal antiboby combined with an immunomodulatory agent and a proteasome inhibitor plus dexamethasone. Funding: Janssen and Celgene. © 2020 Elsevier Ltd
- Published
- 2020
14. Matrix fraction approach for finite-state aerodynamic modeling
- Author
-
Morino, L., Mastroddi, F., De Troia, R., Ghiringhelli, G.L., and Mantegazza, P.
- Subjects
Aerodynamics -- Models ,Structural analysis (Engineering) -- Matrix methods ,Least squares -- Usage ,Aerospace and defense industries ,Business - Abstract
A least-square matrix-fraction procedure, used in finite-state approximation of an aerodynamic matrix, is used to explain aerodynamics in a finite-state form. It involves minimization of a quadratic functional and is a very simple and efficient method, as compared to Karpel's. The method makes use of an approximation, writing an aerodynamic matrix as ND(super -1), with N and D matrices of polynomial dependence.
- Published
- 1995
15. S872 CARFILZOMIB LENALIDOMIDE DEXAMETHASONE (KRD) WITH OR WITHOUT TRANSPLANTATION IN NEWLY DIAGNOSED MYELOMA (FORTE TRIAL): EFFICACY ACCORDING TO RISK STATUS
- Author
-
Gay, F., primary, Cerrato, C., additional, Rota Scalabrini, D., additional, Belotti, A., additional, Galli, M., additional, Zamagni, E., additional, Offidani, M., additional, Omedé, P., additional, Monaco, F., additional, Tosi, P., additional, Annibali, O., additional, Pisani, F., additional, Troia, R., additional, Pascarella, A., additional, Ferrara, F., additional, Cea, M., additional, Dalla Palma, B., additional, Patriarca, F., additional, Aquino, S., additional, Palmas, A., additional, Siniscalchi, A., additional, Grasso, M., additional, Palumbo, A., additional, Ledda, A., additional, Musto, P., additional, Cavo, M., additional, and Boccadoro, M., additional
- Published
- 2019
- Full Text
- View/download PDF
16. PF727 DOES QUALITY OF LIFE IN MULTIPLE MYELOMA PATIENTS DIFFER BETWEEN NATIONALITIES? A EUROPEAN MULTINATIONAL STUDY
- Author
-
Dongen - Leunis, A. Van, primary, Valent, E., additional, Morgan, K., additional, Sajjad, A., additional, Troia, R., additional, Vallejo, A., additional, Sonneveld, P., additional, and Redekop, K., additional
- Published
- 2019
- Full Text
- View/download PDF
17. Autologous transplant vs oral chemotherapy and lenalidomide in newly diagnosed young myeloma patients: a pooled analysis
- Author
-
Gay, F, primary, Oliva, S, additional, Petrucci, M T, additional, Montefusco, V, additional, Conticello, C, additional, Musto, P, additional, Catalano, L, additional, Evangelista, A, additional, Spada, S, additional, Campbell, P, additional, Ria, R, additional, Salvini, M, additional, Offidani, M, additional, Carella, A M, additional, Omedé, P, additional, Liberati, A M, additional, Troia, R, additional, Cafro, A M, additional, Malfitano, A, additional, Falcone, A P, additional, Caravita, T, additional, Patriarca, F, additional, Nagler, A, additional, Spencer, A, additional, Hajek, R, additional, Palumbo, A, additional, and Boccadoro, M, additional
- Published
- 2016
- Full Text
- View/download PDF
18. MbCO Embedded in Trehalosyldextrin Matrices: Thermal Effects and Protein–Matrix Coupling Food Biophysics
- Author
-
Giuffrida S, Troia R, D'AGOSTINO, Antonella, Cordone L., SCHIRALDI, Chiara, DE ROSA, Mario, Giuffrida, S, Troia, R, Schiraldi, Chiara, D'Agostino, Antonella, DE ROSA, Mario, and Cordone, L.
- Published
- 2011
19. Biopreservation of model proteins embedded in maltodextrins- and trehalosildextrins–water matrices
- Author
-
GIUFFRIDA, Sergio, CORDONE, Lorenzo, D'AGOSTINO, Antonino, TROIA R, SCHIRALDI, C, PANARIELLO A., GIUFFRIDA S, TROIA R, CORDONE L, SCHIRALDI, DAGOSTINO A, and PANARIELLO A
- Published
- 2005
20. Finite-State Modeling for Flutter Suppression and Gust Alleviation
- Author
-
DE TROIA, R., Mastroddi, Franco, Gennaretti, M., Morino, Luigi, R., De Troia, F., Mastroddi, Gennaretti, Massimo, and L., Morino
- Published
- 1996
21. Gust Response of a Flexible Wing-Tail Configuration
- Author
-
DE TROIA, R., Gennaretti, M., Morino, Luigi, Mastroddi, Franco, and Pecora, M.
- Published
- 1995
22. On the Finite-State and Observability of Aeroservoelastic Systems
- Author
-
Mastroddi, Franco, DE TROIA, R., Morino, Luigi, and Pecora, M.
- Published
- 1994
23. On Finite-State Aerodynamic Modeling with Applications to Aeroservoelasticity
- Author
-
Morino, Luigi, Mastroddi, Franco, DE TROIA, R., and Pecora, M.
- Published
- 1993
24. Diagnosis of sepsis in dogs by measuring carbonylated proteins (PCOs) and paraoxonase (PON-1).
- Author
-
Ruggerone, B., Troia, R., Murgia, E., Giunti, M., Dondi, F., and Paltrinieri, S.
- Subjects
- *
SEPSIS , *DOG diseases , *CARBONYLATION - Abstract
An early diagnosis of sepsis could allow a better prognosis and avoid the abuse of antibiotic administration; unfortunately, in veterinary medicine, specific and sensitive markers of sepsis are not available. Because Protein Carbonyls (PCOs), that result from protein oxidation, are widely used in human medicine as sepsis markers (Abu-Zidan et al., 2002; Colombo et al., 2015), the aim of our study was to validate an ELISA kit (Enzolifesciences, 3V Chimica, Roma) on canine serum and to measure PCOs, after a preliminary validation study, in three groups (homogeneous for age and size): healthy dogs without clinical or laboratory abnormalities (A, n=14), dogs with septic (B, n=14) and non-septic inflammation (C, n=12) at the first presentation and without previous treatments. Moreover, Paraoxonase-1, a negative acute phase protein with anti-oxidant properties (PON-1) was measured in each group with a method already validated in dogs (Rossi et al., 2013). A Kruskal-Wallis test followed by a Wilcoxon signed rank test was used to evaluate differences between groups. The ELISA method for measuring PCOs showed a very good precision (coefficient of variation <12%) and a good accuracy in spiking-recovery tests. Compared with controls, the concentration of PCOs was significantly higher either in dogs with sepsis (P<0.001) or in dogs with non-septic inflammation (P=0.005) but no significant differences were found between the two groups of sick dogs (Figure 1a). Conversely, PON-1 was significantly lower in sick dogs compared with controls (P<0.001 for both groups) and in septic dogs compared with dogs with non-septic inflammation (P=0.001) (Figure 1b). A negative correlation between the two markers was found (P<0.001, r=-0.594) Receiver operating characteristic (ROC) curves demonstrated that both markers may discriminate dogs with sepsis with the other groups. However, PCO was less sensitive than PON-1 in diagnosing sepsis. Future studies should be focused on the association of PCOs with other inflammatory markers, as well as the possible prognostic role of PCOs based on the outcome of the enrolled patients. [ABSTRACT FROM AUTHOR]
- Published
- 2018
25. LENALIDOMIDE-DEXAMETHSONE (RD) VS MELPHALAN-LENALIDOMIDE-PREDNISONE (MPR) VS CYCLOPHOSPHAMIDE-PREDNISONE-LENALIDOMIDE (CPR) IN ELDERLY COMMUNITY-BASED NEWLY DIAGNOSED MYELOMA PATIENTS
- Author
-
Magarotto, V., Bringhen, S., Roman Hajek, Musto, P., Goldaniga, M. C., Ledda, A., Rosa, L., Patriarca, F., Morabito, F., Troia, R., Genuardi, M., Berno, T., Gambella, M., Pescosta, N., Maisnar, V., Galli, M., Falcone, A. P., Rossi, D., Offidani, M., Boccadoro, M., and Palumbo, A.
26. UPFRONT OR RESCUE TRANSPLANT IN YOUNG PATIENTS WITH NEWLY DIAGNOSED MULTIPLE MYELOMA: A POOLED ANALYSIS OF 529 PATIENTS
- Author
-
Gay, F., Magarotto, V., Spencer, A., Di Raimondo, F., Pour, L., Scudla, V., Genuardi, M., Carella, A. M., Liberati, A. M., Spada, S., Evangelista, A., Omede, P., Nagler, A., Zambello, R., Troia, R., Rossi, G., Offidani, M., Oliva, S., Malfitano, A., Ben Yehuda, D., Patriarca, F., Salvini, M., Corradini, P., Foa, R., Cascavilla, N., Pulini, S., Petrucci, M. T., Roman Hajek, Boccadoro, M., and Palumbo, A.
27. Minimum-impact-on-classifier (MIC) watermarking for protection of remote sensing imagery
- Author
-
Barni, M., primary, Magli, E., additional, and Troia, R., additional
- Full Text
- View/download PDF
28. Minimum-Impact-on-Classifier (MIC) watermarking for protection of remote sensing imagery.
- Author
-
Barni, M., Magli, E., and Troia, R.
- Published
- 2004
- Full Text
- View/download PDF
29. Canine circovirus and Canine adenovirus type 1 and 2 in dogs with parvoviral enteritis
- Author
-
Mara Battilani, Alessia Terrusi, Silvia A. M. Stefanelli, Roberta Troia, Lorenza Urbani, Andrea Balboni, Massimo Giunti, and Balboni A, Terrusi A, Urbani L, Troia R, Stefanelli SAM, Giunti M, Battilani M
- Subjects
Circovirus ,Parvovirus, Canine ,viruses ,Adenoviruses, Canine ,Canine adenovirus, Canine circovirus, Canine parvovirus, Coinfection, Dog, Enteritis ,Canine adenovirus ,Canine circovirus ,Enteritis ,Dogs ,Dog ,medicine ,Animals ,Dog Diseases ,Circoviridae Infections ,Canine parvovirus ,Phylogeny ,Feces ,General Veterinary ,biology ,Coinfection ,Parvovirus ,General Medicine ,biology.organism_classification ,medicine.disease ,Virology ,Breed ,Population study ,Original Article ,Purebred - Abstract
Canine parvovirus type 2 (CPV-2) is one of the most relevant pathogens associated with enteritis in dogs and is frequently reported in association with the detection of other pathogens in faeces. In this study the concomitant presence of Canine circovirus (CanineCV) and Canine adenovirus (CAdV) DNA in faecal or intestine samples of 95 dogs with parvovirus enteritis sampled in Italy (1995–2017) was investigated and the viruses identified were genetically characterised. Potential correlations with the antigenic variant of CPV-2 and with signalment data and outcome were evaluated. Twenty-eight of 95 (29.5%) CPV-2 infected dogs tested positive to other viruses: 7/28 were also positive to CanineCV, 1/28 to CAdV-1, 18/28 to CAdV-2, 1/28 to CanineCV and CAdV-2, and 1/28 to CAdV-1 and CAdV-2. The frequency of CAdV DNA detection and coinfections was significantly higher in purebred dogs compared to mixed breed ones (P = 0.002 and 0.009, respectively). The presence of coinfection was not associated with any other relevant data available, including CPV-2 variant and final outcome. The detection of CanineCV in a dog sampled in 2009 allowed to backdating its circulation in dogs. The eight CanineCV completely sequenced were phylogenetically related to the CanineCV identified in dogs, wolves and a badger from Europe, USA, Argentina and China. Nine CAdV were partially sequenced and phylogenetic analysis showed a separate branch for the oldest CAdV-2 identified (1995). From the results obtained in this study population, CanineCV and CAdV coinfections in dogs with parvoviral enteritis did not result in more severe disease. Supplementary Information The online version contains supplementary material available at 10.1007/s11259-021-09850-y.
- Published
- 2021
30. Concomitant Infections With Canine Parvovirus Type 2 and Intracellular Tick-Borne Pathogens in Two Puppy Dogs
- Author
-
Lorenza Urbani, Alessandro Tirolo, Andrea Balboni, Roberta Troia, Francesco Dondi, Mara Battilani, Urbani L., Tirolo A., Balboni A., Troia R., Dondi F., and Battilani M.
- Subjects
co-infection ,General Veterinary ,dog ,Ehrlichia cani ,Hepatozoon cani ,vector-borne disease ,parvovirus enteriti - Abstract
In this report the concomitant infection with canine parvovirus type 2 (CPV-2), Hepatozoon canis and Ehrlichia canis in two puppy dogs from Southern Italy is described. Dogs were referred to a veterinary university hospital for the acute onset of lethargy and gastrointestinal signs. A complete clinical and clinicopathological evaluation was carried out and the multiple infection was confirmed by microscopic detection of inclusion bodies in peripheral blood smear, rapid immunoenzymatic tests, indirect fluorescent antibody tests, and molecular assays. Sequence analysis revealed that the CPV-2 identified belonged to the 2c variant and had amino acid residues in the predicted VP2 protein typical of “Asian-like” strains widespread in Asia and occasionally reported in Romania, Nigeria and Italy, particularly in the region of Sicily. Numerous monocytes were infected by both H. canis gamonts and E. canis morulae, suggesting that this co-infection is not accidental and that E. canis preferably infects those cells parasitized by H. canis. The clinical presentation of these animals was severe but supportive cares associated with early etiological therapy allowed a good prognosis. Movement of puppies from geographic areas where vector-borne pathogens are endemic must be carefully evaluated and core vaccinations and ectoparasite prevention treatments must be rigorously adopted.
- Published
- 2022
31. Autologous haematopoietic stem-cell transplantation versus bortezomib-melphalan-prednisone, with or without bortezomib-lenalidomide-dexamethasone consolidation therapy, and lenalidomide maintenance for newly diagnosed multiple myeloma (EMN02/HO95)
- Author
-
Michele Cavo, Francesca Gay, Meral Beksac, Lucia Pantani, Maria Teresa Petrucci, Meletios A Dimopoulos, Luca Dozza, Bronno van der Holt, Sonja Zweegman, Stefania Oliva, Vincent H J van der Velden, Elena Zamagni, Giuseppe A Palumbo, Francesca Patriarca, Vittorio Montefusco, Monica Galli, Vladimir Maisnar, Barbara Gamberi, Markus Hansson, Angelo Belotti, Ludek Pour, Paula Ypma, Mariella Grasso, Alexsandra Croockewit, Stelvio Ballanti, Massimo Offidani, Iolanda D Vincelli, Renato Zambello, Anna Marina Liberati, Niels Frost Andersen, Annemiek Broijl, Rossella Troia, Anna Pascarella, Giulia Benevolo, Mark-David Levin, Gerard Bos, Heinz Ludwig, Sara Aquino, Anna Maria Morelli, Ka Lung Wu, Rinske Boersma, Roman Hajek, Marc Durian, Peter A von dem Borne, Tommaso Caravita di Toritto, Thilo Zander, Christoph Driessen, Giorgina Specchia, Anders Waage, Peter Gimsing, Ulf-Henrik Mellqvist, Marinus van Marwijk Kooy, Monique Minnema, Caroline Mandigers, Anna Maria Cafro, Angelo Palmas, Susanna Carvalho, Andrew Spencer, Mario Boccadoro, Pieter Sonneveld, Hematology, CCA - Cancer Treatment and quality of life, RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, Interne Geneeskunde, MUMC+: MA Hematologie (9), Immunology, Cavo M., Gay F., Beksac M., Pantani L., Petrucci M.T., Dimopoulos M.A., Dozza L., van der Holt B., Zweegman S., Oliva S., van der Velden V.H.J., Zamagni E., Palumbo G.A., Patriarca F., Montefusco V., Galli M., Maisnar V., Gamberi B., Hansson M., Belotti A., Pour L., Ypma P., Grasso M., Croockewit A., Ballanti S., Offidani M., Vincelli I.D., Zambello R., Liberati A.M., Andersen N.F., Broijl A., Troia R., Pascarella A., Benevolo G., Levin M.-D., Bos G., Ludwig H., Aquino S., Morelli A.M., Wu K.L., Boersma R., Hajek R., Durian M., von dem Borne P.A., Caravita di Toritto T., Zander T., Specchia G., Waage A., Gimsing P., Mellqvist U.-H., van Marwijk Kooy M., Minnema M., Mandigers C., Cafro A.M., Palmas A., Carvalho S., Spencer A., Boccadoro M., and Sonneveld P.
- Subjects
Male ,Melphalan ,Gastrointestinal Diseases ,multiple myeloma, ASCT, consolidation therapy, EMN02/HO95 ,Dexamethasone ,Bortezomib ,0302 clinical medicine ,Maintenance therapy ,Antineoplastic Combined Chemotherapy Protocols ,Medicine ,Lenalidomide ,Multiple myeloma ,education.field_of_study ,Hematopoietic Stem Cell Transplantation ,Hematology ,Middle Aged ,daratumumab ,Myeloma Proteins ,SINGLE ,030220 oncology & carcinogenesis ,Administration, Intravenous ,Female ,Multiple Myeloma ,medicine.drug ,Rare cancers Radboud Institute for Health Sciences [Radboudumc 9] ,medicine.medical_specialty ,Neutropenia ,Injections, Subcutaneous ,Population ,Infections ,Transplantation, Autologous ,Disease-Free Survival ,03 medical and health sciences ,All institutes and research themes of the Radboud University Medical Center ,Internal medicine ,Humans ,education ,Neoplasm Staging ,Performance status ,business.industry ,DELETION ,medicine.disease ,Thrombocytopenia ,Consolidation Chemotherapy ,Transplantation ,Prednisone ,business ,Plasmacytoma ,DARATUMUMAB ,030215 immunology - Abstract
Background The emergence of highly active novel agents has led some to question the role of autologous haematopoietic stem-cell transplantation (HSCT) and subsequent consolidation therapy in newly diagnosed multiple myeloma. We therefore compared autologous HSCT with bortezomib-melphalan-prednisone (VMP) as intensification therapy, and bortezomib-lenalidomide-dexamethasone (VRD) consolidation therapy with no consolidation.Methods In this randomised, open-label, phase 3 study we recruited previously untreated patients with multiple myeloma at 172 academic and community practice centres of the European Myeloma Network. Eligible patients were aged 18-65 years, had symptomatic multiple myeloma stage 1-3 according to the International Staging System (I S S), measurable disease (serum M protein >10 g/L or urine M protein >200 mg in 24 h or abnormal free light chain [FLC] ratio with involved FLC >100 mg/L, or proven plasmacytoma by biopsy), and WHO performance status grade 0-2 (grade 3 was allowed if secondary to myeloma). Patients were first randomly assigned (1:1) to receive either four 42-day cycles of bortezomib (1.3 mg/m 2 administered intravenously or subcutaneously on days 1, 4, 8, 11, 22, 25, 29, and 32) combined with melphalan (9 mg/m(2) administered orally on days 1-4) and prednisone (60 mg/m(2) administered orally on days 1-4) or autologous HSCT after high-dose melphalan (200 mg/m(2)), stratified by site and ISS disease stage. In centres with a double HS CT policy, the first randomisation (1:1:1) was to VMP or single or double HSCT. Afterwards, a second randomisation assigned patients to receive two 28-day cycles of consolidation therapy with bortezomib (1.3 mg/m(2)either intravenously or subcutaneously on days 1, 4, 8, and 11), lenalidomide (25 mg orally on days 1-21), and dexamethasone (20 mg orally on days 1, 2, 4, 5, 8, 9, 11, and 12) or no consolidation; both groups received lenalidomide maintenance therapy (10 mg orally on days 1-21 of a 28-day cycle). The primary outcomes were progression-free survival from the first and second randomisations, analysed in the intention-to-treat population, which included all patients who underwent each randomisation. All patients who received at least one dose of study drugs were included in the safety analyses. This study is registered with the EU Clinical Trials Register (EudraCT 2009-017903-28) and ClinicalTrials.gov (NCT01208766), and has completed recruitment.Findings Between Feb 25, 2011, and April 3, 2014, 1503 patients were enrolled. 1197 patients were eligible for the first randomisation, of whom 702 were assigned to autologous HSCT and 495 to VMP; 877 patients who were eligible for the first randomisation underwent the second randomisation to VRD consolidation (n=449) or no consolidation (n=428). The data cutoff date for the current analysis was Nov 26, 2018. At a median follow-up of 60.3 months (IQR 52. 2-67. 6), median progression-free survival was significantly improved with autologous HSCT compared with VMP (56.7 months [95% CI 49.3-64.5] vs 41.9 months [37.5-46.9]; hazard ratio [HR] 0.73, 0.62-0.85; p=0.0001). For the second randomisation, the number of events of progression or death at data cutoff was lower than that preplanned for the final analysis; therefore, the results from the second protocol-specified interim analysis, when 66% of events were reached, are reported (data cutoff Jan 18, 2018). At a median follow-up of 42.1 months (IQR 32.3-49.2), consolidation therapy with VRD significantly improved median progression-free survival compared with no consolidation (58.9 months [54.0-not estimable] vs 45.5 months [39.5-58.4]; HR 0.77, 0.63-0.95; p=0.014). The most common grade >= 3 adverse events in the autologous HSCT group compared to the VMP group included neutropenia (513 [79%] of 652 patients vs 137 [29%] of 472 patients), thrombocytopenia (541 [83%] vs 74 [16%]), gastrointestinal disorders (80 [12%] vs 25 [5%]), and infections (192 [30%] vs 18 [4%]). 239 (34%) of 702 patients in the autologous HSCT group and 135 (27%) of 495 in the VMP group had at least one serious adverse event. Infection was the most common serious adverse event in each of the treatment groups (206 [56%] of 368 and 70 [37%] of 189). 38 (12%) of 311 deaths from first randomisation were likely to be treatment related: 26 (68%) in the autologous HSCT group and 12 (32%) in the VMP group, most frequently due to infections (eight [21%]), cardiac events (six [16%]), and second primary malignancies (20 [53%]).Interpretation This study supports the use of autologous HSCT as intensification therapy and the use of consolidation therapy in patients with newly diagnosed multiple myeloma, even in the era of novel agents. The role of high-dose chemotherapy needs to be reassessed in future studies, in particular in patients with undetectable minimal residual disease after four-drug induction regimens including a monoclonal antiboby combined with an immunomodulatory agent and a proteasome inhibitor plus dexamethasone. Copyright (C) 2020 Elsevier Ltd. All rights reserved.
- Published
- 2020
32. Effect of sampling time on urinary electrolytes following oral furosemide administration in dogs with myxomatous mitral valve disease
- Author
-
M.C. Sabetti, F. Fidanzio, R. Troìa, L. Perissinotto, G. Romito, C. Mazzoldi, C. Quintavalla, S. Crosara, F. Dondi, Sabetti M.C., Fidanzio F., Troia R., Perissinotto L., Romito G., Mazzoldi C., Quintavalla C., Crosara S., and Dondi F.
- Subjects
General Veterinary ,Physiology ,Natriuresi ,Sodium ,Heart Valve Diseases ,Heart disease ,Urine chemistry ,Electrolytes ,Dogs ,Fractional excretion ,Furosemide ,Animals ,Humans ,Mitral Valve ,Diuretic ,Dog Diseases ,Diuretics - Abstract
Introduction/Objectives: Urine chemistry has received growing attention to estimate the diuretic response in dogs with cardiac disease. The aim of the study was to evaluate the impact of time elapsed between the oral furosemide administration and sample collection on urine chemistry in dogs with myxomatous mitral valve disease (MMVD) receiving diuretic therapy in American College of Veterinary Internal Medicine (ACVIM) stage C. Materials and methods: Seventy-three dogs with MMVD ACVIM stage C and 106 healthy dogs were prospectively included. Dogs with MMVD were divided, based on the time of sampling, in morning group (MMVD-MG) of one to 6 h and an evening group (MMVD-EG) over 6 h from oral furosemide administration. Analogously, healthy dogs sampled between 9 a.m. and 1 p.m. and between 2 and 7 p.m. were divided in a morning group (H-MG) and an evening group (H-EG), respectively. Urine chemistry, including fractional excretion of electrolytes, was evaluated and compared among groups. Results: Higher excretion of sodium and chloride and higher urine sodium to urine potassium ratio (uNa+:uK+) were detected in MMVD-MG than MMVD-EG (p = 0.021, p = 0.038, and p = 0.016, respectively). Natriuresis, chloriuresis, and uNa+:uK+ were higher in MMVD-MG than H-MG, while no differences were found in the comparison between H-MG and H-EG and between MMVD-EG and H-EG. Conclusions: Urinary electrolyte excretion is significantly increased within 6 h from furosemide administration in MMVD ACVIM stage C dogs. Time of sampling from furosemide administration significantly affects urine chemistry in MMVD dogs and should be considered in clinical practice and the research field.
- Published
- 2022
33. Consolidation and Maintenance in Newly Diagnosed Multiple Myeloma
- Author
-
Pieter Sonneveld, Meletios A. Dimopoulos, Meral Beksac, Bronno van der Holt, Sara Aquino, Heinz Ludwig, Sonja Zweegman, Thilo Zander, Elena Zamagni, Ruth Wester, Roman Hajek, Lucia Pantani, Luca Dozza, Francesca Gay, AnneMaria Cafro, Luca De Rosa, Annamaria Morelli, Henrik Gregersen, Nina Gulbrandsen, Petra Cornelisse, Rosella Troia, Stefania Oliva, Vincent van de Velden, KaLung Wu, Paula F. Ypma, Gerard Bos, Mark-David Levin, Luca Pour, Christoph Driessen, Annemiek Broijl, Alexandra Croockewit, Monique C. Minnema, Anders Waage, Cecilie Hveding, Niels W. C. J. van de Donk, Massimo Offidani, Giuseppe A. Palumbo, Andrew Spencer, Mario Boccadoro, Michele Cavo, Interne Geneeskunde, MUMC+: MA Hematologie (9), RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, Hematology, Immunology, CCA - Cancer Treatment and quality of life, Sonneveld P., Dimopoulos M.A., Beksac M., van der Holt B., Aquino S., Ludwig H., Zweegman S., Zander T., Zamagni E., Wester R., Hajek R., Pantani L., Dozza L., Gay F., Cafro A., De Rosa L., Morelli A., Gregersen H., Gulbrandsen N., Cornelisse P., Troia R., Oliva S., van de Velden V., Wu K., Ypma P.F., Bos G., Levin M.-D., Pour L., Driessen C., Broijl A., Croockewit A., Minnema M.C., Waage A., Hveding C., van de Donk N.W.C.J., Offidani M., Palumbo G.A., Spencer A., Boccadoro M., and Cavo M.
- Subjects
Oncology ,Cancer Research ,Neoplasm, Residual ,Time Factors ,THERAPY ,Consolidation (business) ,Antineoplastic Combined Chemotherapy Protocols ,IMPROVES ,Lenalidomide/administration & dosage ,Lenalidomide ,Multiple myeloma ,OUTCOMES ,INDUCTION ,Antineoplastic Combined Chemotherapy Protocols/administration & dosage ,Middle Aged ,OPEN-LABEL ,Progression-Free Survival ,DEXAMETHASONE ,Europe ,Residual ,Multiple Myeloma ,Human ,Rare cancers Radboud Institute for Health Sciences [Radboudumc 9] ,Adult ,Dexamethasone/administration & dosage ,medicine.medical_specialty ,Time Factor ,Adolescent ,Multiple Myeloma/drug therapy ,BORTEZOMIB ,Newly diagnosed ,Maintenance Chemotherapy ,Young Adult ,SDG 3 - Good Health and Well-being ,Internal medicine ,medicine ,Humans ,Aged ,Neoplasm Staging ,Antineoplastic Combined Chemotherapy Protocol ,business.industry ,STEM-CELL TRANSPLANTATION ,medicine.disease ,Consolidation Chemotherapy ,LENALIDOMIDE MAINTENANCE ,Bortezomib/administration & dosage ,Neoplasm ,CONSENSUS ,business - Abstract
PURPOSE To address the role of consolidation treatment for newly diagnosed, transplant eligible patients with multiple myeloma in a controlled clinical trial. PATIENTS AND METHODS The EMN02/HOVON95 trial compared consolidation treatment with two cycles of bortezomib, lenalidomide, and dexamethasone (VRD) or no consolidation after induction and intensification therapy, followed by continuous lenalidomide maintenance. Primary study end point was progression-free survival (PFS). RESULTS Eight hundred seventy-eight eligible patients were randomly assigned to receive VRD consolidation (451 patients) or no consolidation (427 patients). At a median follow-up of 74.8 months, median PFS with adjustment for pretreatment was prolonged in patients randomly assigned to VRD consolidation (59.3 v 42.9 months, hazard ratio [HR] = 0.81; 95% CI, 0.68 to 0.96; P = .016). The PFS benefit was observed across most predefined subgroups, including revised International Staging System (ISS) stage, cytogenetics, and prior treatment. Revised ISS3 stage (HR, 2.00; 95% CI, 1.41 to 2.86) and ampl1q (HR, 1.67; 95% CI, 1.37 to 2.04) were significant adverse prognostic factors. The median duration of maintenance was 33 months (interquartile range 13-86 months). Response ≥ complete response (CR) after consolidation versus no consolidation before start of maintenance was 34% versus 18%, respectively ( P < .001). Response ≥ CR on protocol including maintenance was 59% with consolidation and 46% without ( P < .001). Minimal residual disease analysis by flow cytometry in a subgroup of 226 patients with CR or stringent complete response or very good partial response before start of maintenance demonstrated a 74% minimal residual disease–negativity rate in VRD-treated patients. Toxicity from VRD was acceptable and manageable. CONCLUSION Consolidation treatment with VRD followed by lenalidomide maintenance improves PFS and depth of response in newly diagnosed patients with multiple myeloma as compared to maintenance alone.
- Published
- 2021
34. Classification of Septic Shock Phenotypes Based on the Presence of Hypotension and Hyperlactatemia in Cats
- Author
-
Massimo Giunti, Ilaria Magagnoli, Francesco Dondi, Francesca Buzzurra, Roberta Troia, Elena Ciuffoli, Giulia Mascalzoni, Armando Foglia, Troia R., Buzzurra F., Ciuffoli E., Mascalzoni G., Foglia A., Magagnoli I., Dondi F., and Giunti M.
- Subjects
medicine.medical_specialty ,multi-organ dysfunction syndrome ,Veterinary medicine ,Sepsis ,Internal medicine ,SF600-1100 ,Medicine ,feline ,dysoxic ,cryptic ,Original Research ,CATS ,General Veterinary ,business.industry ,Septic shock ,Mortality rate ,Organ dysfunction ,medicine.disease ,Blood pressure ,Shock (circulatory) ,vasoplegic ,Cardiology ,Veterinary Science ,Hyperlactatemia ,medicine.symptom ,business - Abstract
Background: Three different phenotypes of septic shock based on changes in blood pressure and lactate are recognized in people. Dysoxic shock, representing the combination of fluid-refractory hypotension and hyperlactatemia, is characterized by greater disease severity and mortality compared to cryptic shock (hyperlactatemia alone) and vasoplegic shock (hypotension with normal blood lactate). Little is known about septic shock and specifically its phenotypes in cats.Objective: To analyze the characteristics and prognostic implications of three septic shock phenotypes in cats with sepsis.Methods: Cats with septic shock were prospectively included. Septic shock was defined by the presence of hypotension (mean blood pressure 4 mmol/L) and classified in three subgroups: dysoxic shock, vasoplegic shock and cryptic shock. Clinical and clinicopathological variables including APPLEfast and APPLEfull scores, occurrence of multi-organ dysfunction syndrome (MODS; presence of at least two dysfunctional organs simultaneously) and outcome were compared among subgroups. Cats with sepsis showing normal blood pressure and lactate concentrations hospitalized during the study period were included as uncomplicated sepsis, and compared to cats with septic shock for selected variables. Length of hospital stay and mortality were evaluated in the whole study population. Odds ratios for mortality were calculated using logistic regression analysis. Significance was set at P < 0.05.Results: The study enrolled 48 cats with uncomplicated sepsis and 37 cats with septic shock (dysoxic shock n = 17; vasoplegic shock n = 11; cryptic shock n = 7). Cats with dysoxic shock had significantly higher APPLEfast and APPLEfull scores compared to vasoplegic and cryptic shock. Mortality rates were not significantly different among cryptic (57%), dysoxic (65%) and vasoplegic shock (91%), while MODS occurrence was significantly lower in cats with cryptic shock (57%) compared to patients affected by dysoxic (94%) and vasoplegic (100%) shock. Cats with septic shock had higher frequency of MODS and greater mortality rate than cats with uncomplicated sepsis.Conclusion: Despite similar in-hospital mortality, cats with dysoxic and vasoplegic shock are characterized by having higher occurrence of multi- organ dysfunction compared to cats affected by cryptic shock. Results from this study suggest novel means of identifying high-risk subgroups of septic cats.
- Published
- 2021
35. Minimal residual disease assessment by multiparameter flow cytometry in transplant-eligible myeloma in the EMN02/HOVON 95 MM trial
- Author
-
Claudia Brandt-Hagens, Stefania Oliva, Pavla Všianská, Maria Teresa Petrucci, Roman Hájek, Romana Jugooa, Monica Galli, Mattia D'Agostino, Rossella Ribolla, Davine Hofste op Bruinink, Pieter Sonneveld, Andrea Capra, Bronno van der Holt, Tania Villanova, Massimo Offidani, Michele Cavo, Lucia Pantani, Rossella Troia, Mario Boccadoro, Paola Omedè, Lucie Rihova, Milena Gilestro, Vincent H.J. van der Velden, Oliva S., Bruinink D.H., Rihova L., D'Agostino M., Pantani L., Capra A., van der Holt B., Troia R., Petrucci M.T., Villanova T., Vsianska P., Jugooa R., Brandt-Hagens C., Gilestro M., Offidani M., Ribolla R., Galli M., Hajek R., Gay F., Cavo M., Omede P., van der Velden V.H.J., Boccadoro M., Sonneveld P., Hematology, and Immunology
- Subjects
Melphalan ,Male ,medicine.medical_specialty ,Neoplasm, Residual ,Population ,Urology ,Bone Marrow Cells ,Article ,Dexamethasone ,Disease-Free Survival ,Bortezomib ,EuroFlow ,hemic and lymphatic diseases ,Antineoplastic Combined Chemotherapy Protocols ,Autograft ,Humans ,Medicine ,Autografts ,education ,Lenalidomide ,Multiple myeloma ,RC254-282 ,education.field_of_study ,Antineoplastic Combined Chemotherapy Protocol ,medicine.diagnostic_test ,business.industry ,Hematopoietic Stem Cell Transplantation ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Hematology ,Translational research ,Middle Aged ,medicine.disease ,Flow Cytometry ,Minimal residual disease ,body regions ,Survival Rate ,medicine.anatomical_structure ,Risk factors ,Oncology ,Bone Marrow Cell ,Female ,Bone marrow ,business ,Multiple Myeloma ,medicine.drug ,Fluorescence in situ hybridization ,Human - Abstract
Minimal residual disease (MRD) by multiparameter flow cytometry (MFC) is the most effective tool to define a deep response in multiple myeloma (MM). We conducted an MRD correlative study of the EMN02/HO95 MM phase III trial in newly diagnosed MM patients achieving a suspected complete response before maintenance and every 6 months during maintenance. Patients received high-dose melphalan (HDM) versus bortezomib-melphalan-prednisone (VMP) intensification, followed by bortezomib-lenalidomide-dexamethasone (VRd) versus no consolidation, and lenalidomide maintenance. Bone marrow (BM) samples were processed in three European laboratories, applying EuroFlow-based MFC protocols (eight colors, two tubes) with 10−4−10−5 sensitivity. At enrollment in the MRD correlative study, 76% (244/321) of patients were MRD-negative. In the intention-to-treat analysis, after a median follow-up of 75 months, 5-year progression-free survival was 66% in MRD-negative versus 31% in MRD-positive patients (HR 0.39; p p
- Published
- 2021
36. Comparison of Protein Carbonyl (PCO), Paraoxonase-1 (PON1) and C-Reactive Protein (CRP) as Diagnostic and Prognostic Markers of Septic Inflammation in Dogs
- Author
-
Francesco Dondi, Roberta Troia, Saverio Paltrinieri, Donatella Scavone, Beatrice Ruggerone, Massimo Giunti, Ruggerone B., Scavone D., Troia R., Giunti M., Dondi F., and Paltrinieri S.
- Subjects
medicine.medical_specialty ,acute phase protein ,canine ,inflammation ,oxidative stress ,prognosis ,sepsis ,Prognosi ,Veterinary medicine ,Inflammation ,Acute phase protein ,Gastroenterology ,Article ,Canine ,Sepsis ,Internal medicine ,SF600-1100 ,Medicine ,General Veterinary ,biology ,Receiver operating characteristic ,business.industry ,C-reactive protein ,Paraoxonase ,Acute-phase protein ,medicine.disease ,PON1 ,biology.protein ,Mann–Whitney U test ,Oxidative stre ,medicine.symptom ,business - Abstract
Reliable diagnostic and prognostic markers of sepsis are lacking, but essential in veterinary medicine. We aimed to assess the accuracy of C-Reactive Protein (CRP), protein carbonyls (PCO) and paraoxonase-1 (PON1) in differentiating dogs with sepsis from those with sterile inflammation and healthy ones, and predict the outcome in septic dogs. These analytes were retrospectively evaluated at admission in 92 dogs classified into healthy, septic and polytraumatized. Groups were compared using the Kruskal–Wallis test, followed by a Mann–Whitney U test to assess differences between survivors and non-survivors. Correlation between analytes was assessed using the Spearman’s test, and their discriminating power was assessed through a Receiver Operating Characteristic (ROC) curve. PON1 and CRP were, respectively, significantly lower and higher in dogs with sepsis compared with polytraumatized and clinically healthy dogs (p < 0.001 for both the analytes), and also in dogs with trauma compared with healthy dogs (p = 0.011 and p = 0.017, respectively). PCO were significantly increased in septic (p < 0.001) and polytraumatized (p < 0.005) as compared with healthy dogs. PON1 and CRP were, respectively, significantly lower and higher in dogs that died compared with survivors (p < 0.001 for both analytes). Ultimately, evaluation of CRP and PON1 at admission seems a reliable support to diagnose sepsis and predict outcomes.
- Published
- 2021
- Full Text
- View/download PDF
37. Heinz body–related interference with leukocyte and erythrocyte variables obtained by an automated hematology analyzer in cats
- Author
-
Chiara Agnoli, Federica Serafini, Roberta Troia, Massimo Giunti, Francesco Dondi, Marta Gruarin, Kateryna Vasylyeva, Dondi F., Vasylyeva K., Serafini F., Gruarin M., Troia R., Giunti M., and Agnoli C.
- Subjects
Male ,medicine.medical_specialty ,Erythrocytes ,spurious leukocytosis ,Sensitivity and Specificity ,Gastroenterology ,hematology cytogram ,Leukocyte Count ,peroxidase reaction ,Internal medicine ,White blood cell ,Leukocytes ,Animals ,Medicine ,Full Scientific Reports ,feline ,Heinz Bodies ,Retrospective Studies ,Hematologic Tests ,Hematology ,CATS ,General Veterinary ,Mean corpuscular hemoglobin concentration ,medicine.diagnostic_test ,business.industry ,Reproducibility of Results ,Complete blood count ,Flow Cytometry ,Blood Cell Count ,Red blood cell ,medicine.anatomical_structure ,Cats ,Female ,Hemoglobin ,business ,Heinz body - Abstract
Heinz bodies (HBs) are known to interfere with automated hematology in cats, particularly with the white blood cell (WBC) count. We evaluated the influence of feline HBs on the complete blood count (CBC) results obtained using a flow cytometry–based analyzer. We retrospectively selected cats with circulating HBs and reviewed the results of their CBCs, including red blood cell (RBC) indices, basophil/lobularity (Baso) WBC count (WBCB), peroxidase (Perox) WBC count (WBCP), and cytograms. Based on the presence or absence of HB-related artifacts in their Baso cytogram, cats were grouped into Baso-HBs and HBs groups, respectively, for comparison. The WBCB and WBCP were compared to manual counts of WBCs carried out on blood smears at 400× (MC-WBC). We included 32 cats in our study: 9 of 32 were in the Baso-HBs group, and 23 of 32 were in the HBs group. Baso-HBs cats had a significantly increased HB percentage ( p < 0.001), WBCB ( p < 0.001), difference between WBCB and WBCP ( p < 0.001), lymphocyte count ( p < 0.001), mean corpuscular hemoglobin concentration ( p < 0.001), and difference between calculated and measured erythrocyte hemoglobin concentrations ( p < 0.001) compared to HBs cats. In Baso-HBs cats, the WBCB was significantly higher than the WBCP ( p = 0.02); no significant difference was detected between the WBCP and the MC-WBC ( p = 0.88). Evaluation of automated CBC results raised the suspicion of HB-related interference when using a hematology analyzer in cats; hence, blood smear examination remains essential in routine practice.
- Published
- 2019
38. Reversible myocardial dysfunction in a dog after resuscitation from cardiopulmonary arrest
- Author
-
Ilaria Magagnoli, Massimo Giunti, G. Romito, Roberta Troia, E. Murgia, Magagnoli I., Romito G., Troia R., Murgia E., and Giunti M.
- Subjects
Resuscitation ,medicine.medical_specialty ,Cardiac troponin ,040301 veterinary sciences ,Physiology ,Mandibular fracture ,Sedation ,medicine.medical_treatment ,Myocardial lesion ,030204 cardiovascular system & hematology ,Return of spontaneous circulation ,Ventricular Function, Left ,Canine ,0403 veterinary science ,03 medical and health sciences ,Ventricular Dysfunction, Left ,0302 clinical medicine ,Internal medicine ,medicine ,Dog ,Cardiopulmonary resuscitation ,Cardiomyopathie ,Skull radiography ,General Veterinary ,business.industry ,Animal ,Cardiac troponin I ,04 agricultural and veterinary sciences ,medicine.disease ,Cardiopulmonary Resuscitation ,Heart Arrest ,Myocardial injury ,Cardiology ,medicine.symptom ,Dog Disease ,business ,Intravenous pimobendan - Abstract
A 4-year-old Dachshund was referred for management of a mandibular fracture. The dog underwent cardiopulmonary arrest after sedation for skull radiography. Cardiopulmonary resuscitation was started immediately, and return of spontaneous circulation was rapidly obtained. However, after resuscitation, the dog was hemodynamically unstable. Additionally, global left ventricular systolic dysfunction and a focal hyperechoic myocardial lesion were found echocardiographically, and serum cardiac troponin I was severely elevated (82.80 ng/mL, upper hospital limit
- Published
- 2021
39. Stage 1-Biomarkers of Kidney Injury in Dogs Undergoing Constant Rate Infusion of Hydroxyethyl Starch 130/0.4
- Author
-
Cristiana Maurella, Francesco Dondi, Ilaria Lippi, Roberta Troia, Paola Gianella, Antonio Borrelli, Alberto Tarducci, Barbara Bruno, Bruno B., Troia R., Dondi F., Maurella C., Gianella P., Lippi I., Tarducci A., and Borrelli A.
- Subjects
medicine.medical_specialty ,Veterinary medicine ,Rate of infusion ,Urinary system ,Urology ,Renal function ,Hydroxyethyl starch ,Article ,tubular damage ,Excretion ,chemistry.chemical_compound ,SF600-1100 ,medicine ,urinary neutrophil gelatinase-associated lipocalin ,Hypoalbuminemia ,reproductive and urinary physiology ,Creatinine ,General Veterinary ,business.industry ,Albumin ,medicine.disease ,hydroxyethyl starch ,Renal biomarker ,QL1-991 ,chemistry ,Animal Science and Zoology ,renal biomarkers ,biological phenomena, cell phenomena, and immunity ,business ,Zoology ,medicine.drug - Abstract
In veterinary medicine, investigations relating the effects of hydroxyethyl starch (HES) on renal function report contrasting results. This study aimed to assess the changes in the selected biomarkers of kidney injury in dogs after the administration of HES 130/0.4 as a constant rate infusion (CRI) for 24 h. Ten adult client-owned dogs with hypoalbuminemia (albumin <, 2 g/dL) and ongoing fluid losses were included. Enrolled dogs received intravenous fluid therapy with crystalloids and a CRI of HES 130/0.4 at a dose of 2 mL/kg/h for 24 h. Serum creatinine (sCr), fractional excretion (FE) of electrolytes, urinary protein to creatinine ratio (UPC), urinary albumin to creatinine ratio (UAC), SDS-page, and urinary neutrophil gelatinase-associated lipocalin (uNGAL) were measured at the baseline before HES infusion, and after 24 h (T24) and 48 h (T48) from the baseline. No statistically significant difference was found between the baseline value vs. T24 and the baseline vs. T48 for sCr, UAC, UPC, FE of sodium, chloride and calcium, and uNGAL. A significant increase in FEK (p = 0.04) was noticed between the baseline and T48. In this study sample of hypoalbuminemic dogs, HES 130/0.4 at the dose and rate of infusion applied did not cause any significant changes in the investigated biomarkers of kidney injury.
- Published
- 2021
40. Cytokine and Chemokine Profiling in Cats With Sepsis and Septic Shock
- Author
-
Giulia Mascalzoni, Roberta Troia, Robert Goggs, Massimo Giunti, Chiara Agnoli, Denise F. LaLonde‐Paul, Troia R., Mascalzoni G., Agnoli C., Lalonde-Paul D., Giunti M., and Goggs R.
- Subjects
Chemokine ,040301 veterinary sciences ,medicine.medical_treatment ,0403 veterinary science ,Sepsis ,03 medical and health sciences ,RANTES ,Immune system ,medicine ,feline ,KC-Like ,Interleukin 6 ,Original Research ,030304 developmental biology ,0303 health sciences ,IL-6 ,CATS ,lcsh:Veterinary medicine ,General Veterinary ,biology ,IL-8 ,Septic shock ,business.industry ,interleukin ,Interleukin ,04 agricultural and veterinary sciences ,medicine.disease ,multiplex ,Cytokine ,Immunology ,biology.protein ,lcsh:SF600-1100 ,Veterinary Science ,business - Abstract
Background: Sepsis is a life-threatening condition associated with an exacerbated production of both pro- and anti-inflammatory cytokines that can promote a hyperactive response to infection or induce immunoparalysis. Data regarding the immune response to sepsis in cats are scarce. Establishing the profiles of cytokines and chemokines in feline sepsis to characterize the nature of the immune responses to sepsis might enable individualized treatments to be developed and targeted. Objective: To evaluate the cytokine and chemokine network in cats with sepsis and septic shock, and to investigate the associations of these analytes with disease severity and outcome. Methods: Blood samples prospectively collected at presentation of cats with sepsis and septic shock to two veterinary teaching hospitals were analyzed. Forty healthy cats were included as controls. A 19-plex feline cytokine/chemokine magnetic bead assay system was used to measure analytes in citrated plasma samples. Cytokine concentrations were compared between groups using the Kruskal-Wallis test with Dunn's post-hoc correction for multiple comparisons. Cytokine concentrations were compared between survivors and non-survivors with the Mann-Whitney U test. Odds ratios were calculated using logistic regression. A multivariable logistic regression model for prediction of septic shock was constructed. Results: The study enrolled 35 septic cats. Many cytokines were undetectable in both sick and healthy control cats and were excluded from subsequent analyses. Comparisons of cytokine concentrations among healthy controls, cats with sepsis (n = 12) and cats with septic shock (n = 23) revealed that sick cats (sepsis or septic shock) had significantly higher plasma concentrations of IL-6, IL-8, KC-like, and RANTES compared to healthy controls. The combination of MCP-1, Flt-3L, and IL-12 was predictive of septic shock. None of the cytokines analyzed was predictive of outcome in this study population. Conclusion: Plasma concentrations of IL-6, IL-8, KC-like, and RANTES are increased in cats with sepsis and may play important roles in pathogenesis. Multivariable modeling suggested that analysis of cytokines might aid differentiation of septic shock from sepsis. None of the cytokines analyzed was predictive of outcome. Measurement of these cytokines might enable future studies to better diagnose and characterize feline sepsis and septic shock.
- Published
- 2020
41. Evaluation of Serum Apolipoprotein A1 in Canine Sepsis
- Author
-
Massimo Giunti, Roberta Troia, Giorgio Grossi, Francesco Dondi, Federico Fracassi, Giunti M., Grossi G., Troia R., Fracassi F., and Dondi F.
- Subjects
high-density lipoproteins ,medicine.medical_specialty ,dogs ,Serum albumin ,Peritonitis ,high-density lipoprotein ,Gastroenterology ,Enteritis ,Sepsis ,Internal medicine ,acute phase response ,medicine ,Prospective cohort study ,Original Research ,lcsh:Veterinary medicine ,General Veterinary ,biology ,septic peritonitis ,business.industry ,Acute-phase protein ,Pyometra ,medicine.disease ,dog ,biology.protein ,lcsh:SF600-1100 ,Apolipoprotein A1 ,Veterinary Science ,prognosis ,business ,prognosi - Abstract
Decreased serum apolipoprotein A1 (Apo-A1) concentration is associated with mortality in human sepsis. The diagnostic and prognostic role of serum Apo-A1 concentrations in canine sepsis was evaluated. Serum samples from septic dogs (n = 91) and healthy controls (n = 15) were retrospectively analyzed. According to the sepsis origin, four categories were identified: parvoviral enteritis (n = 26), pyometra (n = 20), septic peritonitis (n = 19), and miscellanea (n = 26). The canine acute patient physiologic and laboratory evaluation fast score (APPLEfast), serum C-reactive protein (CRP) and albumin concentrations were reviewed in all enrolled dogs. Increased CRP (252.6 ± 119.2 mg/L; Reference Interval: 0–8.5 mg/L) and significant lower serum albumin and Apo-A1 concentrations were documented in dogs with sepsis (22.8 ± 5.3 g/L and 1.17 ± 0.27 g/L, respectively) compared to healthy ones (33.1 ± 2.5 g/L and 1.32 ± 0.05 g/L, respectively) (P < 0.0001). According to the origin of sepsis, only the subgroup of dogs with septic peritonitis had significantly lower Apo-A1 (1.03 ± 0.26 g/L) concentrations compared to healthy dogs (P < 0.001). No significant differences were found in serum albumin and CRP concentrations, and in APPLEfast score values among the different subgroups of sepsis. Diagnosis of septic peritonitis was associated with a higher frequency of death (P = 0.006). In septic dogs, significant lower Apo-A1 concentrations were detected in non-survivors (1.02 ± 0.28 g/L; n = 27) compared to survivors (1.23 ± 0.24 g/L; n = 64; P = 0.0007). Moreover, significant higher values of the APPLEfast score were calculated in non-survivors (26 ± 4; n = 19) compared to survivors (23 ± 4; n = 51) (P = 0.0114). According to the area under the ROC curve analysis, Apo-A1
- Published
- 2020
42. Circulating Methemoblogin Fraction in Dogs With Sepsis
- Author
-
Massimo Giunti, Francesco Dondi, Elena Ciuffoli, Armando Foglia, Kateryna Vasylyeva, Roberta Troia, Troia R., Ciuffoli E., Vasylyeva K., Foglia A., Dondi F., and Giunti M.
- Subjects
medicine.medical_specialty ,040301 veterinary sciences ,canine ,Methemoglobinemia ,Gastroenterology ,Methemoglobin ,Nitric oxide ,0403 veterinary science ,Sepsis ,sepsis ,03 medical and health sciences ,chemistry.chemical_compound ,nitric oxide ,Internal medicine ,hemic and lymphatic diseases ,medicine ,methemoglobin ,Original Research ,030304 developmental biology ,0303 health sciences ,lcsh:Veterinary medicine ,General Veterinary ,business.industry ,Septic shock ,Organ dysfunction ,Retrospective cohort study ,04 agricultural and veterinary sciences ,medicine.disease ,chemistry ,CO-oximetry ,lcsh:SF600-1100 ,septic shock ,Veterinary Science ,sepsi ,Hemoglobin ,medicine.symptom ,business - Abstract
Large amount of nitric oxide (NO) can be released in patients with sepsis. Methemoglobin is formed from the interaction between NO and hemoglobin. Mild methemoglobinemia reflecting NO overproduction has been reported in septic people, and occasionally associated to septic shock and organ dysfunction. The aim of this retrospective study was to evaluate circulating methemoglobin fraction in dogs with sepsis and to assess its prognostic value. Methemoglobin reference interval (RI) was calculated in 41 healthy dogs and was set at 0–2.2%. A total of 131 dogs with sepsis were included in the study; 24/131 had a circulating methemoglobin ≥2.2%. The median methemoglobin fraction was significantly higher in dogs with sepsis compared to healthy ones (1.7%, 0.4–3.5% vs. 1.0, 0.3–2.2%, P = 0.0005). No significant difference was observed between dogs with uncomplicated sepsis (n = 98) vs. dogs with septic shock (n = 33) (1.8%, 0.4–2.8% vs. 1.5%, 0.4–3.5%, P = 0.74), between dogs with and without multi-organ dysfunction (n = 38 and n = 93, respectively) (1.7%, 0.4–3.5% vs. 1.7%, 0.5–2.8%, P = 0.27), and between survivors (n = 77) vs. non survivors (n = 54) (1.5%, 0.4–2.8% vs. 1.8%, 0.4–3.5%, P = 0.05). Dogs with methemoglobin fraction above or equal to the upper limit of the RI had a significantly higher frequency of death compared to dogs with methemoglobin levels
- Published
- 2020
43. Prognostic Significance of Organ Dysfunction in Cats With Polytrauma
- Author
-
Armando Foglia, Roberta Troia, Francesco Dondi, Cecilia Bulgarelli, Marco Pelizzola, Elsa Murgia, Massimo Giunti, Murgia E., Troia R., Bulgarelli C., Pelizzola M., Foglia A., Dondi F., and Giunti M.
- Subjects
medicine.medical_specialty ,Prognosi ,040301 veterinary sciences ,Population ,Severity scoring system ,shock ,law.invention ,0403 veterinary science ,03 medical and health sciences ,law ,Internal medicine ,medicine ,polytrauma ,feline ,education ,multiple organ dysfunction syndrome ,Original Research ,030304 developmental biology ,0303 health sciences ,education.field_of_study ,lcsh:Veterinary medicine ,General Veterinary ,business.industry ,Mortality rate ,Organ dysfunction ,04 agricultural and veterinary sciences ,medicine.disease ,Polytrauma ,Intensive care unit ,severity scoring systems ,Blunt trauma ,lcsh:SF600-1100 ,Veterinary Science ,prognosis ,medicine.symptom ,business ,Multiple organ dysfunction syndrome ,Penetrating trauma - Abstract
Polytrauma is a common emergency condition in small animals and is frequently associated with higher morbidity and mortality rates compared to minor trauma. Multiple Organ Dysfunction Syndrome (MODS) is a major complication of extensive traumatic injury, carrying a high risk of death despite intensive care treatment. Little is known about the prevalence and the prognostic impact of MODS in feline polytrauma. The current study aimed to prospectively evaluate the occurrence and the prognostic significance of organ dysfunction at admission in a population of polytraumatized cats. Cats with polytrauma requiring intensive care unit hospitalization were included and categorized according to outcome (survivors/non-survivors). Clinical and clinicopathological data, including scores of disease severity [Animal Trauma Triage Score (ATTS), APPLEfast, and APPLEfull], selected organ dysfunction and presence of MODS were evaluated upon admission, and analyzed with respect to mortality. Non-parametric statistics was performed and P < 0.05 was considered significant. Thirty-eight cats met the inclusion criteria: 8/38 (21%) had penetrating trauma, while 30/38 (79%) had blunt trauma. The overall in-hospital mortality was 37% (14/38). Cats with evidence of MODS upon admission had significantly higher frequency of death compared to cats without MODS (9/14 vs. 2/24 P = 0.0004). Hemostatic dysfunction, respiratory dysfunction, and MODS upon admission were significantly associated with mortality in the univariate logistic regression analysis (P = 0.005, P = 0.001, P = 0.001, respectively). The values of APPLEfast, APPLEfull, and ATTS were independently associated with a higher risk of death and positively correlated with the number of dysfunctional organs (P = 0.025, P = 0.004, P = 0.003, r = 0.57, P = 0.0002; r = 0.59, P = 0.0001; r = 0.55, P = 0.0003, respectively). Multiple Organ Dysfunction Syndrome is a common complication of feline polytrauma and its development is associated with increased disease severity and worse outcomes. The presence of hemostatic dysfunction and respiratory dysfunction upon admission is associated with a higher risk of death. The ATTS and the APPLE scores are useful prognostic tools for the assessment of cats with polytrauma.
- Published
- 2019
44. Comparison of clinicopathological patterns of renal tubular damage in dogs with acute kidney injury caused by leptospirosis and other aetiologies
- Author
-
Francesco Dondi, C. Grisetti, S Zamagni, Andrea Balboni, Erika Monari, Roberta Troia, L. Perissinotto, F. Zaccheroni, and Zamagni S, Troia R, Zaccheroni F, Monari E, Grisetti C, Perissinotto L, Balboni A, Dondi F
- Subjects
Male ,Urine glucose ,medicine.medical_specialty ,040301 veterinary sciences ,Urinary system ,Kaliuresi ,030204 cardiovascular system & hematology ,Lipocalin ,Gastroenterology ,Urine chemistry ,0403 veterinary science ,Excretion ,03 medical and health sciences ,chemistry.chemical_compound ,Dogs ,0302 clinical medicine ,Lipocalin-2 ,Electrolyte fractional excretion ,Glycosuria ,Internal medicine ,Animals ,Medicine ,Leptospirosis ,Dog Diseases ,NGAL ,Leptospira ,Creatinine ,General Veterinary ,business.industry ,Acute kidney injury ,04 agricultural and veterinary sciences ,Dipstick ,Acute Kidney Injury ,medicine.disease ,Kidney Tubules ,chemistry ,Kaliuresis ,Potassium ,Female ,Animal Science and Zoology ,business - Abstract
In humans, leptospiral acute kidney injury (AKI) is characterised by tubulointerstitial involvement and renal electrolyte losses, impacting clinical presentation and case management. The aim of this study was to evaluate urine chemistry findings in dogs with leptospirosis in order to identify characteristic patterns of tubular damage associated with this disease. Dogs with intrinsic AKI caused by leptospirosis and by other aetiologies were prospectively enrolled. Clinical and clinicopathological variables, including serum and urine chemistry, fractional excretion (FE%) of electrolytes, and urinary neutrophil gelatinase-associated lipocalin (NGAL), were evaluated in both groups and compared statistically. Dogs with leptospirosis (n = 38) had significantly higher serum creatinine concentration than dogs with AKI caused by other aetiologies (n = 37). Serum potassium and glucose concentrations were comparable between groups. Dogs with leptospiral AKI had significantly higher FE of potassium (median 100%, range 20–480 vs. median 68%, range 5–300; P = 0.048), as well as higher magnitude of glucosuria (urine glucose to creatinine ratio, median 0.64, range 0–26 vs. median 0.22, range 0–13; P = 0.023) and frequency of positive glucose dipstick reaction (59% vs. 18%; P = 0.002), than dogs with AKI of other aetiologies. Additional markers of tubular damage considered in this study, including FE of other electrolytes and urinary NGAL, did not differ between groups. In conclusion, when compared to other aetiologies of intrinsic AKI, canine leptospirosis was characterised by increased glucosuria and kaliuresis.
- Published
- 2020
45. Retrospective evaluation of circulating thyroid hormones in critically ill dogs with systemic inflammatory response syndrome
- Author
-
Luciana Giardino, Giulia Andreani, Roberta Troia, Mara Battilani, Massimo Giunti, Federico Fracassi, Francesco Dondi, Giunti, M, Troia, R, Battilani, M, Giardino, L, Dondi, F, Andreani, G, and Fracassi, F.
- Subjects
0301 basic medicine ,Male ,medicine.medical_specialty ,040301 veterinary sciences ,Critical Illness ,Population ,canine ,Gastroenterology ,0403 veterinary science ,Sepsis ,03 medical and health sciences ,Dogs ,Internal medicine ,medicine ,Animals ,Dog Diseases ,canine, euthyroid sick syndrome, systemic inflammatory response syndrome, thyroid hormones ,education ,Retrospective Studies ,thyroid hormones ,education.field_of_study ,General Veterinary ,business.industry ,Thyroid ,Retrospective cohort study ,04 agricultural and veterinary sciences ,medicine.disease ,euthyroid sick syndrome ,humanities ,Systemic Inflammatory Response Syndrome ,Systemic inflammatory response syndrome ,030104 developmental biology ,medicine.anatomical_structure ,Italy ,Thyroid hormones ,Immunology ,Female ,Original Article ,business ,Euthyroid sick syndrome ,Hormone - Abstract
Critical illness can be associated with transient alterations in circulating thyroid hormone concentrations, indicating the presence of non-thyroidal illness (NTI). NTI is well described in humans, but there are few reports on its occurrence and prognostic significance in dogs. This retrospective study assessed the occurrence of NTI in a population of dogs with systemic inflammatory response syndrome (SIRS) and investigated its association with disease severity (APPLEfast scores). A total of 41 SIRS dogs were included and were divided by SIRS origin (non-septic SIRS, n = 10; septic SIRS, n = 41) and final outcome (survivors, n = 37; non-survivors, n = 4). Healthy, age-matched dogs (n = 15) were included as controls. Serum thyroid hormone levels including total T3, free T3, total T4, and reverse T3 were measured upon admission. Compared to controls, there were significant changes in serum thyroid hormone concentrations in SIRS dogs, suggesting the presence of NTI. Septic SIRS dogs had higher APPLEfast scores and lower serum thyroid hormones concentrations than those in non-septic SIRS and control dogs. In conclusion, NTI was frequent in dogs with SIRS and may be associated with the presence of sepsis or high illness severity
- Published
- 2016
46. MbCO embedded in trehalosyldextrin matrices: thermal effects and protein-matrix coupling
- Author
-
Antonella D’Agostino, Lorenzo Cordone, Mario De Rosa, Chiara Schiraldi, Rosario Troia, Sergio Giuffrida, Giuffrida, S, Troia, R, Schiraldi, C, D'Agostino, A, De Rosa, M, and Cordone, L
- Subjects
chemistry.chemical_classification ,denaturation ,Biophysics ,Infrared spectroscopy ,Trehalose ,Bioengineering ,Applied Microbiology and Biotechnology ,Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin) ,Analytical Chemistry ,Amorphous solid ,chemistry.chemical_compound ,Matrix (mathematics) ,chemistry ,Chemical engineering ,Organic chemistry ,Denaturation (biochemistry) ,Dextrin ,Fourier transform infrared spectroscopy ,Ternary operation ,MbCO ,dextrin ,Food Science ,Settore CHIM/02 - Chimica Fisica - Abstract
Saccharide-based biopreservation is widely studied because of its scientific importance and possible technological outcomes for food and pharmaceutical industries. Ternary protein/saccharide/water systems have been extensively exploited to model the characteristics of the in vivo biopreservation process. A tight, water dependent, protein–matrix coupling has been shown to occur in various simple saccharide amorphous matrices, which is stronger in trehalose. The efficiency as bioprotectant of trehalose has been ascribed to this tight coupling, since the appearance of damages on biological structures will more involve structural variations of the surrounding matrix. Here we present, as an applicative follow-up of this research line, a Fourier transform infrared study on protein–matrix coupling in commercial maltodextrins and trehalosyldextrins solid amorphous systems, with carboxymyoglobin embedded, and compare the results with analogous system containing trehalose and maltose, previously reported. Results point out that trehalosyldextrins are useful candidates as protecting agents, even though with an efficiency lower than trehalose, and could be used when the rheological properties of relative long-chain oligosaccharides are needed. However, it appears that a substantial improvement could be obtained by removal of the small fraction of glucose.
- Published
- 2011
47. A Retrospective Matched-Pair Analysis of Long-term Outcomes after Successful Lower Extremity Free Flap Salvage.
- Author
-
Bigdeli AK, Strübing F, Troia R, Thomas B, Gazyakan E, Kneser U, and Hirche C
- Subjects
- Humans, Retrospective Studies, Cicatrix, Quality of Life, Matched-Pair Analysis, Limb Salvage, Treatment Outcome, Free Tissue Flaps blood supply
- Abstract
Background: Pedicle occlusion with total free flap loss after microvascular lower extremity reconstruction is a considerably rare yet devastating complication. Fortunately, in the majority of cases, emergency salvage takebacks of compromised free flaps are initiated in a timely manner. In this report, we present our analysis of long-term outcomes following transient vascular compromise mitigated through successful free flap salvage in the lower extremity., Methods: We performed a single-center retrospective matched-pair analysis of 46 patients with lower extremity free flap reconstructions. Cases underwent successful revisions of microvascular compromise ( n = 23), whereas controls had uneventful postoperative courses ( n = 23). Patient-reported outcome questionnaires and physical evaluations were used to assess general quality of life, functional outcomes, and cosmesis (Lower Extremity Functional Scale [LEFS], Lower Limb Outcomes Questionnaire [LLOQ], Short Form 36 (SF-36), Vancouver Scar Scale [VSS]). The mean follow-up time was 4.4 years., Results: The health-related quality of life assessed by the SF-36 did not differ significantly between both groups in any of the subscales ( p ≥ 0.15 for all subscales). Functional outcomes did not show significant differences between both groups according to the LEFS ( p = 0.78) and LLOQ ( p = 0.45). The overall scar appearance assessed by the VSS showed significantly poorer cosmesis in the re-exploration group ( p = 0.014)., Conclusion: Salvage of compromised free flaps in the lower extremity yields similar long-term outcomes compared to noncompromised free flaps with regard to function and quality of life. However, free flap revisions may lead to impaired scar formation. This study provides further evidence that the opportunity for urgent re-exploration is indispensable., Competing Interests: None declared., (Thieme. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
48. Apolipoprotein A1 and serum amyloid A in dogs with sepsis and septic shock.
- Author
-
Bulgarelli C, Ciuffoli E, Troia R, Goggs R, Dondi F, and Giunti M
- Abstract
Introduction: Apolipoprotein-A1 (Apo-A1) acts as a negative acute phase protein (APP) during inflammatory states, and has a potential prognostic value in people and dogs with sepsis. The aim of this retrospective study was to investigate the association of serum Apo-A1 concentration with disease severity, multiorgan dysfunction syndrome (MODS) and outcome in a population of dogs with sepsis, and to assess its correlation with major canine APPs., Methods: Ninety-nine dogs with uncomplicated sepsis ( n = 78) or septic shock ( n = 21) were included. The serum concentration of Apo-A1, C-reactive protein (CRP) and serum amyloid A (SAA) were recorded, alongside the canine acute patient physiologic and laboratory evaluation fast (APPLE
fast ) score and the presence of MODS., Results: Dogs with septic shock had significantly lower serum Apo-A1 concentrations (106.3 ± 22.7 mg/dl; reference interval: 123.0-142.3 mg/dl), higher APPLEfast score (30, 13-38) and greater frequency of MODS (67%) compared to those with uncomplicated sepsis (117.9 ± 19.3 mg/dl; 25, 6-33 and 8%, respectively) ( P = 0.0201; P = 0.0005; P < 0.0001, respectively). Similarly, dogs with MODS had significantly lower serum Apo-A1 concentrations (104.1 ± 4.6 mg/dl) and higher APPLEfast score values (31, 13-38) compared to those without MODS (118.32 ± 2.1 mg/dl and 26, 6-33, respectively) ( P = 0.0050 and P = 0.0038, respectively). Conversely, neither CRP nor SAA were different between these groups. No difference in serum APPs concentrations was detected between survivors and non-survivors. Significant negative correlations were detected between serum Apo-A1 and SAA ( P = 0.0056, r = -0.277), and between serum Apo-A1 and the APPLEfast score ( P = 0.0027, r = -0.3). In this population, higher values of the APPLEfast score and the presence of MODS were independently associated with a higher risk of death., Discussion: Our study shows that Apo-A1 is a useful biomarker of sepsis severity in dogs, since it is decreased in those with septic shock and MODS. Further prospective investigations are deemed to evaluate the applicability of Apo-A1 to predict sepsis course and response to treatment in septic dogs., Competing Interests: MG is an associate editor of the journal but only participated in the peer review process as an author. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Bulgarelli, Ciuffoli, Troia, Goggs, Dondi and Giunti.)- Published
- 2023
- Full Text
- View/download PDF
49. Evaluation of urinary neutrophil gelatinase-associated lipocalin to detect renal tubular damage in dogs with stable myxomatous mitral valve disease.
- Author
-
Troia R, Sabetti MC, Crosara S, Quintavalla C, Romito G, Mazzoldi C, Fidanzio F, Cescatti M, Bertazzolo W, Giunti M, and Dondi F
- Subjects
- Dogs, Animals, Lipocalin-2, Mitral Valve, Heart Valve Diseases veterinary, Dog Diseases, Acute Kidney Injury veterinary
- Abstract
Background: Dogs with myxomatous mitral valve disease (MMVD) can experience progressive renal tubular damage and dysfunction. The prevalence of renal tubular damage is not known in dogs with stable MMVD., Objective: To evaluate renal tubular damage in dogs with stable MMVD by evaluation of urinary neutrophil gelatinase-associated lipocalin (NGAL)., Animals: Ninety-eight MMVD dogs grouped according to the American College of Veterinary Internal Medicine (ACVIM) staging (group B1, n = 23; group B2, n = 27; group C + D, n = 48) and 46 healthy dogs., Methods: Multicenter prospective observational study. Serum and urine chemistry including NGAL reported as uNGAL concentration (uNGAL) and normalized with urinary creatinine (uNGALC) were compared between MMVD dogs and healthy controls, and among different MMVD ACVIM stages., Results: The MMVD dogs had significantly higher uNGAL and uNGALC (1204 pg/mL; range, 30-39 732 and 1816 pg/mg; range, 22-127 693, respectively) compared to healthy dogs (584 pg/mL; range, 56-4072 and 231 pg/mg; range, 15-2407, respectively; P = .002 and P < .0001, respectively). Both uNGAL and uNGALC increased with the increasing ACVIM stage (P = .001 and P < .001, respectively)., Conclusions and Clinical Importance: Renal tubular damage is present in dogs with stable MMVD, as measured by increased uNGAL. This tubular damage is subclinical, occurs in all stages of MMVD even in the absence of azotemia, and increases with the severity of MMVD. Reno-protective approaches to manage MMVD dogs should be explored to slow the progression of renal tubular damage in these patients., (© 2022 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.)
- Published
- 2022
- Full Text
- View/download PDF
50. Carfilzomib, Pomalidomide, and Dexamethasone As Second-line Therapy for Lenalidomide-refractory Multiple Myeloma.
- Author
-
Sonneveld P, Zweegman S, Cavo M, Nasserinejad K, Broijl A, Troia R, Pour L, Croockewit S, Corradini P, Patriarca F, Wu K, Droogendijk J, Bos G, Hajek R, Teresa Petrucci M, Ypma P, Zojer N, Minnema MC, and Boccadoro M
- Abstract
This phase 2 trial investigated reinduction with carfilzomib, pomalidomide, and dexamethasone (KPd) and continuous pomalidomide/dexamethasone in patients at first progression during lenalidomide maintenance. The second objective was to evaluate high-dose melphalan with autologous stem cell transplantation (HDM/ASCT) at first progression. Patients were eligible who had progressive disease according to International Myeloma Working Group (IMWG) criteria. Treatment consisted of 8 cycles carfilzomib (20/36 mg/m
2 ), pomalidomide (4 mg) and dexamethasone. Patients without prior transplant received HDM/ASCT. Pomalidomide 4 mg w/o dexamethasone was given until progression. One hundred twelve patients were registered of whom 86 (77%) completed 8 cycles of KPd. Thirty-five (85%) eligible patients received HDM/ASCT. The median time to discontinuation of pomalidomide w/o dexamethasone was 17 months. Best response was 37% ≥ complete response, 75% ≥ very good partial response, 92% ≥ partial response, respectively. At a follow-up of 40 months median PFS was 26 and 32 months for patients who received KPd plus HDM/ASCT and 17 months for patients on KPd (hazard ratio [HR] 0.61, 95% confidence interval [CI] 0.37-1.00, P = 0.051). PFS was better after longer duration of prior lenalidomide (HR 3.56, 95% CI 1.42-8.96, P = 0.035). Median overall survival (OS) was 67 months. KPd-emerging grade 3 and 4 adverse events included hematologic (41%), cardiovascular (6%), respiratory (3%), infections (17%), and neuropathy (2%). KPd followed by continuous pomalidomide is an effective and safe triple drug regimen in second-line for patients previously exposed to bortezomib and/or refractory to lenalidomide., (Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the European Hematology Association.)- Published
- 2022
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.