1. A new approach in tandem mass spectrometric simultaneous determination of perchlorate, thiocyanate and iodide using their ternary complexes with tris(1,10-phenanthroline) iron(II) chelate
- Author
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Kayoko Minakata, Itaru Yamagishi, Koutaro Hasegawa, Hideki Nozawa, Masako Suzuki, Kunio Gonmori, Amin Wurita, Osamu Suzuki, and Kanako Watanabe
- Subjects
chemistry.chemical_classification ,Iodide ,Thiocyanate ,Chemistry ,Tandem mass spectrometry ,Phenanthroline ,Electrospray ionization ,Biochemistry (medical) ,Inorganic chemistry ,Selected reaction monitoring ,Perchlorate ,Toxicology ,Mass spectrometry ,Elecrospray ionization ,Pathology and Forensic Medicine ,chemistry.chemical_compound ,Chelation ,Tris (1,10-phenanthroline) iron (II) - Abstract
A rapid and sensitive method was developed for simultaneous determination of perchlorate (ClO4 −), thiocyanate (SCN−), and iodide (I−). The ions were reacted with tris(1,10-phenanthroline)iron(II) chelating cation, [Fe(phen)3]2+, to produce their ternary complexes, [Fe(phen)2ClO4]+, [Fe(phen)2SCN]+, and [Fe(phen)2I]+ that were extracted together with nitrobenzene. Five microliters of the extract was injected directly into an electrospray ionization tandem mass spectrometer. Quantification of ClO4 −, SCN−, and I− was performed by selected reaction monitoring of the product ion [Fe(phen)O2]+ at m/z 268 that derived from the precursor ion [Fe(phen)2ClO4]+ at m/z 515, the product ion [Fe(phen)SCN]+ at m/z 294 that derived from precursor ion [Fe(phen)2SCN]+ at m/z 474, and the product ion [Fe(phen)I]+ at m/z 363 that derived from precursor ion [Fe(phen)2I]+ at m/z 543, respectively. Perchlorate, SCN−, and I− were measured in the range of 0.1–10 μM with the limits of detection at 0.03 μM by using a 50-μl sample aliquot containing the three ions within 10 min. The SCN− level in gastric fluid of a victim of NaCN poisoning was 137 ± 23 μM, which is slightly higher than the results from control subjects (n = 7), 72 ± 39 μM.
- Published
- 2012