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Copyright of Journal of Agriculture & Nature / Kahramanmaraş Sütçü İmam Üniversitesi Tarım & Doğa Dergisi is the property of Kahramanmaras Sutcu Imam Universitesi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Edible mushrooms are one of the important food source because of their tastes, and having high concentration of fatty acids and the other nutrients. In this study, fatty acid composition and antioxidant properties of Tricholoma imbricatum and T. focale were determined. The fatty acids were analysed by GC, and GC-MS while the antioxidant activity was tested using five complimentary methods; namely, β-carotene-linoleic acid, DPPH scavenging, ABTS scavenging, CUPRAC and metal chelating assays. Eight fatty acids were determined in hexane extract, and oleic acid was the major fatty acid with 46.4% and 35.0%, respectively. Palmitic acid (12.8%, and 5.12%) and lineloic acid (28.2% and 31.0%) were also detected in high amounts. In DPPH.scavenging and ABTS·+ scavenging assays, the methanol extract of T. imbricatum (IC50: 0.12±0.01 mg/mL for both assay) showed better antioxidant activity than those of T. focale (IC50>0.8±0.01, and IC50: 0.21±0.01 mg/mL). All extracts of T. focale exhibited good activity in β-carotene-linoleic acid assay while only the hexane extract of T. imbricatum showed activity. Both mushroom indicated moderate in cupric reducing power. Since the mushrooms are nontoxic and edible, both can be used in food industry as preservatives.

Two undescribed triterpenes, tricholimbrins A and B, three undescribed steroids, tricholimbrins C‒E, one undescribed 4-chromanone derivative, along with 27 known compounds were isolated from fruiting bodies of the mushroom Tricholoma imbricatum. Tricholimbrins A and B are two polycyclic triterpenoids with a carbon degradation, while tricholimbrin C is a ring-rearranged steroid containing an aromatic moiety that might be derived from an ergosterol. Isocyathisterol, 3 β ,15 α -dihydroxyl-(22 E ,24 R)-ergosta-5,8(14),22-trien-7-one, demethylincisterol A 3 , and volemolide showed cytotoxicities to six human cancer cell lines. 3 β -Hydroxyl-(22 E ,24 R)-ergosta-5,8,22-trien-7,15-dione and 3 β -hydroxyl-(22 E ,24 R)-ergosta-5,8,22-trien-7-one showed preferable cytotoxicities against HL-60 while chaxine C and volemolide showed preferable cytotoxicities against A-549, with IC 50 values less than 10 μ M. Triterpenoids and ergosterols from fruiting bodies of Tricholoma imbricatum. Image 1 • A total of 33 compounds have been obtained from mushroom Tricholoma imbricatum. • Compounds 1–3 are rare natural triterpenoids or ergosterol derivatives. • Many compounds possess certain cytotoxicities to human cancer cell lines. [ABSTRACT FROM AUTHOR]