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Tree nuts and oily fruits are used as a diet complement and are highly consumed worldwide. The production and consumption of these foods have been increasing, and an enormous global market value is forecasted for 2023. Besides their high nutritional value and lipid content, they provide health benefits to fat metabolism, heart, skin, and brain. The industrial by-products of these oily foods represent promising raw materials for many industries. However, the lipidomic analysis of nuts and oily fruits is still in its early stages. State-of-the-art analytical approaches for the lipid profiling and fingerprinting of nuts and oily fruits have been developed using high-performance liquid chromatography and high-resolution mass spectrometry for the accurate identification and structural characterization at the molecular species level. It is expected to bring a new understanding of these everyday foods' nutritional and functional value. This review comprises the oil content and lipid composition of various nuts and oily fruits, particularly those mostly consumed worldwide and having recognized beneficial health effects, biological activities associated with the lipids from different oily foodstuffs, analytical methodologies to analyze lipids in nuts and oily fruits, and the potential biotechnological applications of their industrial by-products for a lipid-based commercial valorization. [ABSTRACT FROM AUTHOR]

Archachatina marginata is an African pulmonate gastropod widely reared for food. There is a lack of information on the influence of seasonal changes on the reproductive physiology of these snails, hindering optimum reproduction throughout the year. Thus, the aim of this study was to measure seasonal variation in the albumen gland of A. marginata snails in Abeokuta, Ogun State, Nigeria. A total of 252 snails was purchased from the Department of Forestry and Wildlife Management of Federal University of Agriculture, Abeokuta, Ogun State, Nigeria. The snails were acclimatised for three weeks in rearing cages. Twenty-one snails were dissected each month for twelve months and the albumen gland separated. Samples collected were analysed in the laboratory using standard chemical methods. Data collected were analysed using one-way analysis of variance and t-test. Glucose (P < 0.05) and protein (non-significant) were found to be higher in the dry season than in the rainy season, while cholesterol (P < 0.05) and triacylglycerol (non-significant) were higher in the rainy season than in the dry. In conclusion, this study showed that the biochemical composition of the albumen gland of A. marginata snails was influenced by seasonal variation. [ABSTRACT FROM AUTHOR]

Several studies have indicated a potential causal relationship between plasma standard lipids, such as high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), triglycerides (TG), and total cholesterol (TC), and psoriasis. However, few studies have offered causal evidence of lipid species beyond these standard lipids. We conducted an analysis using a genome-wide association study (GWAS) dataset comprising 179 lipid species, including 13 types across four major categories, to identify instrumental variables (IVs) associated with plasma lipids. We utilized two GWAS datasets from the IEU and Finngen for psoriasis vulgaris as the outcome. A two-sample Mendelian randomization (MR) analysis was used to explore the causal relationship between 179 lipid species and psoriasis vulgaris in two datasets. Lipid species showing causal association in both psoriasis datasets were compared for overlap. Our study identified potential causal relationships between six lipid species and psoriasis vulgaris: phosphatidylcholine (16:1_18:2), phosphatidylcholine (18:0_18:2), phosphatidylcholine (18:1_20:4), phosphatidylethanolamine (16:0_18:2), phosphatidylinositol (18:0_20:3), and triacylglycerol (50:1). In summary, elevated plasma levels of phosphatidylcholine (16:1_18:2), phosphatidylcholine (18:0_18:2), phosphatidylethanolamine (16:0_18:2), phosphatidylinositol (18:0_20:3), and triacylglycerol (50:1) may increase the risk of psoriasis vulgaris. Conversely, plasma phosphatidylcholine (18:1_20:4) may play a protective role against psoriasis vulgaris. [ABSTRACT FROM AUTHOR]

The effects of four initial culture pH values (3, 4, 5, and 6) and nitrogen limitation on growth, TAG accumulation, lipid production, fatty acid profile, and estimated biodiesel quality of Starmerella magnoliae X3 were investigated. TAG and lipid levels were measured by Nile Red fluorescence and sulfo-phospho-vanilin (SPV) techniques, respectively. The results showed that a combination of nitrogen limitation and acidic pH significantly (p < 0.05) increased TAG accumulation, total lipid contents, and lipid productivity in Starmerella magnoliae X3 compared to the control group. Under nitrogen limitation, the highest TAG accumulation was achieved at initial pHs of 3 and 5 after 72 h of cultivation, and the highest lipid productivity (0.306 g L−1 d−1) was observed after 48 h at pH 3; the major fatty acids at the four pH values were oleic acid (63.6%–64%), palmitoleic acid (11.3%–12.5%), stearic acid (9.7%–11.4%), and palmitic acid (9.4%–10%). In addition, both stresses were associated with lower iodine value and higher cetane number of the biodiesel compared to the control. These findings suggest that cultivation in a low-nitrogen medium at an initial pH of 3 or 5 holds promise in increasing TAG production in Starmerella magnoliae X3. [ABSTRACT FROM AUTHOR]

We investigated whether the elimination of two major enzymes responsible for triacylglycerol synthesis altered the structure and physical state of organelle membranes under mild heat shock conditions in the fission yeast, Schizosaccharomyces pombe. Our study revealed that key intracellular membrane structures, lipid droplets, vacuoles, the mitochondrial network, and the cortical endoplasmic reticulum were all affected in mutant fission yeast cells under mild heat shock but not under normal growth conditions. We also obtained direct evidence that triacylglycerol-deficient cells were less capable than wild-type cells of adjusting their membrane physical properties during thermal stress. The production of thermoprotective molecules, such as HSP16 and trehalose, was reduced in the mutant strain. These findings suggest that an intact system of triacylglycerol metabolism significantly contributes to membrane protection during heat stress. [ABSTRACT FROM AUTHOR]

Hypothesis: The physical and mechanical properties of triacylglycerols (TAGs), or 'fats', depend on their composition and thermal history which, in turn, impact crystal structure and morphology. We examine whether thermal history can be mechanistically related to film removal by a surfactant solution. Experiments: Model TAG mixtures, comprising triolein:tripalmitin:tristearin 0.5:0.3:0.2, were subjected to a range of cooling profiles from the melt (0.5-80 °C/min, Newtonian and annealed), and the resulting solid films characterised by microscopy, X-ray diffraction, infrared spectroscopy, and contact angle measurements. Film removal from a model glass substrate by an aqueous surfactant solution of sodium dodecylsulphate and dodecyldimethylamine oxide at room temperature fixed at 25 °C was examined under quiescent flow conditions. Findings: Quantitative relations are established between TAG cooling profile, crystal structure and morphology, surface energy γ S F E , and removal (or 'cleaning'). In general, films cooled slowly from the melt yield heterogeneous morphologies with predominantly β 1 ′ phase, higher polar γ S F E , and faster removal timescales. By contrast, rapid cooling results in homogeneous films, rich in β 2 ′ phase, low polar γ S F E , and long removal times. Our results elucidate the non-trivial impact of TAG thermal history, connecting the multiscale semi-crystalline structure to surface energy, and eventually to film delamination by micellar solutions. [ABSTRACT FROM AUTHOR]

In the present study, the fatty acid composition (FAC), triacylglycerol (TAG) composition, crystallization, and melting behaviors of mango kernel fats (MKFs) extracted from seven Ivorian mango varieties—Amelie (AM), Kent (KT), Palmer (PR), Keitt (KI), Brooks (BR), Dadiani (DI), and Djakoumankoun (DN)—were characterized by complementary techniques: pNMR, DSC, polarized light microscopy, and X-ray diffraction. Regardless of the variety, Ivorian MKF is rich in stearic (St) and oleic (O) fatty acids. (72–84% of the total FA), with three main TAGs: StOSt (23.9–45.8%), StOO (15.5–25.8%), and StLSt (10.4–12.5%). Their crystallization onset temperature (Tco) ranged from 15 to 20 °C. All samples showed complete melting around 35 °C, except DN (~ 38 °C). The β-polymorph appeared to be the most predominant and stable polymorph for the seven MKFs. The thermal properties investigated were linked to monounsaturated and triunsaturated triacylglycerol content, indicating the significant influence of chemical composition. The fine analysis of the results allows the seven Ivorian varieties to be divided into four groups, based on both their physical properties and thermal behavior, and the StOSt wealth: hard high-StOSt fat (DN), half-hard medium-StOSt fat (DI, BR), soft low-StOSt fat (KT, PR, KI), and very-soft very-low-StOSt fat (AM). The seven investigated mango varieties from Ivory Coast might offer a wide range of applications in food, pharmaceutical, and cosmetic industries. [ABSTRACT FROM AUTHOR]

Favism is a metabolic disease and this study evaluates the effectiveness of palm oil and its triacylglycerol constituent in favism-induced female rats to restore serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation. Animals were 36 female albino rats. They classified to two equal (normal and favism) groups. The normal group was divided into three equal subgroups: the control, palm oil, and triacylglycerol subgroups. The normal rats were given 1 mL of saline, 1 mL of palm oil, and 1 mL of triacylglycerol orally, respectively. The Favism group was classified also into three equal subgroups: the favism group, the favism + palm oil, the Favism + triacylglycerol. The favism rats were given 1 mL of saline, 1 mL of palm oil, and 1 mL of triacylglycerol orally. For four weeks, all treatments were administered orally via oral gavage once daily. The hemoglobin, hematocrite, the blood cells, glucose and glucose-6-phosphate dehydrogenase, and liver function were decreased in favism. Female hormones such as serum luteinizing hormone, follicle stimulating hormone, Estrone, Estriol, 17α-Estradiol, 17β-Estradiol, and Estradiol-17-β-stearate were decreased in favism. Ovarian antioxidants were decreased while ovarian inflammatory markers were increased in favism. Favism induced ovarian DNA apoptosis. Furthermore, oral administration with palm oil or its triacylglycerol constituent in favism-induced female rats restored all these parameters to be approached the control levels. Palm oil restored serum female hormones, ovarian antioxidants, inflammatory markers, and DNA fragmentation in favism-induced female rats and this effect related to oil triacylglycerol constituent. [ABSTRACT FROM AUTHOR]

The antidiabetic drug metformin has a wide range of metabolic effects and may also reduce the risk of obesity-related diseases. The aim of the current study was to investigate if metformin could counteract meldonium-induced fatty liver. Four groups of male C57BL/6J mice were fed a low-fat control diet, or low-fat diets supplemented with metformin, meldonium, or metformin and meldonium for three weeks. Meldonium treatment led to 5.2-fold higher hepatic triacylglycerol (TAG) levels compared to control, and metformin lowered the meldonium-induced lipid accumulation insignificantly by 21%. Mice treated with metformin and meldonium demonstrated significantly lower weight gain, visceral adipose tissue weight and plasma levels of TAG compared to meldonium alone. The hepatic mRNA level of carnitine palmitoyl transferase 1 was increased 2-fold with combined meldonium and metformin treatment compared to meldonium treatment (p < 0.001). Increased hepatic expression of genes involved in fatty acid oxidation and lipid transport was observed in the combination group compared to control, and increased gene expression of the mitochondrial uncoupling protein UCP2 was observed compared to the meldonium group. In addition, the product of fatty acid oxidation, acetylcarnitine, increased in plasma in metformin-treated mice. Altogether, metformin treatment influenced hepatic lipid metabolism and lowered plasma TAG in meldonium-induced fatty liver in mice. [ABSTRACT FROM AUTHOR]

Seed triacylglycerol (TAG), a major component of vegetable oil, consists of a glycerol esterified with three fatty acids. Vegetable oil has industrial applications and is widely used as edible oil. The increasing demand for plant oils, owing to population growth, it is crucial to enhance the oil content in seeds. We found castor WRINKLED1A (RcWRI1A) and R2R3-type MYB domain protein 306 (RcMYB306) which have homology with Arabidopsis WRI1 (AtWRI1) and AtMYB96 which regulate genes involved in fatty acid and TAG synthesis, respectively. These castor genes were separately and jointly overexpressed using seed-specific promoters in an oil crop, camelina (Camelina sativa). Overexpression of RcWRI1A, RcMYB306, or RcWRI1A + RcMYB306 increased the total seed oil content in camelina. However, this increase was not significantly different from that observed during the overexpression of RcWRI1A or/and RcMYB306. RcWRI1A overexpression increased the fatty acid content, including 16:0, 18:2, 18:3. Contrastingly, RcMYB306 overexpression increased the 18:1, 18:2, 18:3, 20:0 and 20:1 fatty acid. In the RcWRI1A + RcMYB306 lines, changes in fatty acid composition demonstrated the combined effects of these transcription factors. These results suggest that RcWRI1A and RcMYB306 can be used to improve the productivity of oil crops. [ABSTRACT FROM AUTHOR]

Since nitrogen limitation is known to be an important trigger of triacylglycerol (TAG) accumulation in most microorganisms, we first assessed the global lipid content of 21 strains derived from Streptomyces coelicolor M145 deleted for genes involved in nitrogen metabolism. Seven of these strains deleted for genes encoding proteins involved in polyamine (GlnA2/SCO2241, GlnA3/SCO6962, GlnA4/SCO1613), or protein (Pup/SCO1646) degradation, in the regulation of nitrogen metabolism (GlnE/SCO2234 and GlnK/SCO5584), or the global regulator DasR/SCO5231 that controls negatively the degradation of N-acetylglucosamine, a constituent of peptidoglycan, had a higher TAG content than the original strain, whereas five of these strains (except the glnA2 and pup mutants) had a lower cardiolipin (CL) content. The production of the blue polyketide actinorhodin (ACT) was totally abolished in the dasR mutant in both Pi conditions, whereas the deletion of pup, glnA2, glnA3, and glnA4 was correlated with a significant increase in total ACT production, but mainly in Pi limitation. Unexpectedly, ACT production was strongly reduced in the glnA3 mutant in Pi proficiency. Altogether, our data suggest that high TAG and ACT biosynthesis and low CL biosynthesis might all contribute to the lowering of oxidative stress resulting from nitrogen limitation or from other causes. [ABSTRACT FROM AUTHOR]

Abstract Seed triacylglycerol (TAG), a major component of vegetable oil, consists of a glycerol esterified with three fatty acids. Vegetable oil has industrial applications and is widely used as edible oil. The increasing demand for plant oils, owing to population growth, it is crucial to enhance the oil content in seeds. We found castor WRINKLED1A (RcWRI1A) and R2R3-type MYB domain protein 306 (RcMYB306) which have homology with Arabidopsis WRI1 (AtWRI1) and AtMYB96 which regulate genes involved in fatty acid and TAG synthesis, respectively. These castor genes were separately and jointly overexpressed using seed-specific promoters in an oil crop, camelina (Camelina sativa). Overexpression of RcWRI1A, RcMYB306, or RcWRI1A + RcMYB306 increased the total seed oil content in camelina. However, this increase was not significantly different from that observed during the overexpression of RcWRI1A or/and RcMYB306. RcWRI1A overexpression increased the fatty acid content, including 16:0, 18:2, 18:3. Contrastingly, RcMYB306 overexpression increased the 18:1, 18:2, 18:3, 20:0 and 20:1 fatty acid. In the RcWRI1A + RcMYB306 lines, changes in fatty acid composition demonstrated the combined effects of these transcription factors. These results suggest that RcWRI1A and RcMYB306 can be used to improve the productivity of oil crops.

Apolipoprotein E is the major lipid transporter in the brain and an important player in neuron-astrocyte metabolic coupling. It ensures the survival of neurons under stressful conditions and hyperactivity by nourishing and detoxifying them. Apolipoprotein E polymorphism, combined with environmental stresses and/or age-related alterations, influences the risk of developing late-onset Alzheimer's disease. In this review, we discuss our current knowledge of how apolipoprotein E homeostasis, i.e. its synthesis, secretion, degradation, and lipidation, is affected in Alzheimer's disease. [ABSTRACT FROM AUTHOR]

Abstract Lignocellulosic biomass is currently underutilized, but it offers promise as a resource for the generation of commercial end-products, such as biofuels, detergents, and other oleochemicals. Rhodococcus opacus PD630 is an oleaginous, Gram-positive bacterium with an exceptional ability to utilize recalcitrant aromatic lignin breakdown products to produce lipid molecules such as triacylglycerols (TAGs), which are an important biofuel precursor. Lipid carbon storage molecules accumulate only under growth-limiting low nitrogen conditions, representing a significant challenge toward using bacterial biorefineries for fuel precursor production. In this work, we screened overexpression of 27 native transcriptional regulators for their abilities to improve lipid accumulation under nitrogen-rich conditions, resulting in three strains that accumulate increased lipids, unconstrained by nitrogen availability when grown in phenol or glucose. Transcriptomic analyses revealed that the best strain (#13) enhanced FA production via activation of the β-ketoadipate pathway. Gene deletion experiments confirm that lipid accumulation in nitrogen-replete conditions requires reprogramming of phenylalanine metabolism. By generating mutants decoupling carbon storage from low nitrogen environments, we move closer toward optimizing R. opacus for efficient bioproduction on lignocellulosic biomass.

Abstract Background The herbaceous peony (Paeonia lactiflora Pall.) is extensively cultivated in China due to its root being used as a traditional Chinese medicine known as ‘Radix Paeoniae Alba’. In recent years, it has been discovered that its seeds incorporate abundant unsaturated fatty acids, thereby presenting a potential new oilseed plant. Surprisingly, little is known about the full-length transcriptome sequencing of Paeonia lactiflora, limiting research into its gene function and molecular mechanisms. Results A total of 484,931 Reads of Inserts (ROI) sequences and 1,455,771 full-Length non-chimeric reads (FLNC) sequences were obtained for CDS prediction, TF analysis, SSR analysis and lncRNA identification. In addition, gene function annotation and gene structure analysis were performed. A total of 4905 transcripts were related to lipid metabolism biosynthesis pathway, belonging to 28 enzymes. We use these data to identify 10 oleosin (OLE) and 5 diacylglycerol acyltransferase (DGAT) gene members after de-redundancy. The analysis of physicochemical properties and secondary structure showed them similarity in gene family respectively. The phylogenetic analysis showed that the distribution of OLE and DGAT family members was roughly the same as that of Arabidopsis. Quantitative real-time polymerase chain reaction (qRT–PCR) analyses revealed expression changes in different seed development stages, and showed a trend of increasing and then decreasing. Conclusion In summary, these results provide new insights into the molecular mechanism of triacylglycerol (TAG) biosynthesis and storage during the seedling stage in Paeonia lactiflora. It provides theoretical references for selecting and breeding oil varieties and understanding the functions of oil storage as well as lipid synthesis related genes in Paeonia lactiflora.

As climate change intensifies and the need to reduce human-caused emissions becomes more urgent, transitioning to a bio-based economy is essential. This paper explores the diverse industrial applications of plant oils as sustainable alternatives to petroleum-based products, including their use in food, polymers, lubricants, surfactants, pesticides, emollients, and biofuels. This review delves into biosynthetic pathways, detailing the key enzymes and processes involved in the synthesis of triacylglycerol. It thoroughly discusses how genetic and metabolic engineering can not only increase oil yields but also modify fatty acid compositions to better meet industrial requirements. By understanding genetics and utilizing advanced biotechnologies, the oil content and quality of plant sources can be significantly enhanced, aligning with both sustainability goals and industrial demands. This paper provides a comprehensive overview of the current uses and genetic engineering of plant oil production, proposing innovative strategies such as utilizing oils from biomass or cultivating non-edible oil crops. These approaches aim to establish a sustainable industrial system, reduce reliance on fossil fuels, and promote the growth of an environmentally responsible bio-based economy. Additionally, the review highlights future directions, examining the economic implications and environmental benefits of adopting plant oils across various sectors and positioning them as pivotal to achieving an eco-friendly, bio-based economy.

SUMMARY: Diacylglycerol acyltransferase1 (DGAT1) is the major enzyme that synthesizes triacylglycerols (TAG) during Arabidopsis seed development. Mutant dgat1 seeds possess low oil content in addition to a high polyunsaturated fatty acid (PUFA) composition. Two genes encoding endoplasmic reticulum localized desaturase enzymes, fatty acid desaturase2 (FAD2) and fatty acid desaturase3 (FAD3), were upregulated in both dgat1‐1 and dgat1‐2 developing seeds. Crosses between both dgat1 mutant alleles and fad2‐1 failed to generate plants homozygous for both dgat1 and fad2. Reciprocal crosses with wild‐type plants demonstrated that both male and female dgat1 fad2 gametophytes were viable. Siliques from DGAT1/dgat1‐1 fad2‐1/fad2‐1 and dgat1‐1/dgat1‐1 FAD2/fad2‐1 possessed abnormal looking seeds that were arrested in the torpedo growth stage. Approximately 25% of the seeds exhibited this arrested phenotype, genetically consistent with them possessing the double homozygous dgat1 fad2 genotype. In contrast, double homozygous dgat1‐1 fad3‐2 mutant plants were viable. Seeds from these plants possessed higher levels of 18:2 while their fatty acid content was lower than dgat1 mutant controls. The results are consistent with a model where in the absence of DGAT1 activity, desaturation of fatty acids by FAD2 becomes essential to provide PUFA substrates for phospholipid:diacylglycerol acyltransferase (PDAT) to synthesize TAG. In a dgat1 fad2 mutant, seed development is aborted because TAG is unable to be synthesized by either DGAT1 or PDAT. Significance Statement: Arabidopsis diacylglycerol acyltransferase1 mutants, defective in their ability to synthesize triacylglycerols (TAG), upregulate fatty acid desaturase2 (FAD2) during seed development, resulting in seed oil with increased polyunsaturated fatty acid content. Double dgat1 fad2 mutants are synthetically lethal due to embryo arrest at the torpedo stage, consistent with a model where enhanced fatty acid desaturation is required to provide substrate for an alternative TAG biosynthetic enzyme, phospholipid:diacylglycerol acyltransferase (PDAT). [ABSTRACT FROM AUTHOR]

Summary: Triacylglycerols (TAG), accumulate within lipid droplets (LD), predominantly surrounded by OLEOSINs (OLE), that protect TAG from hydrolysis. We tested the hypothesis that identifying and removing degradation signals from OLE would promote its abundance, preventing TAG degradation and enhancing TAG accumulation.We tested whether mutating potential ubiquitin‐conjugation sites in a previously reported improved Sesamum indicum OLE (SiO) variant, o3‐3 Cys‐OLE (SiCO herein), would stabilize it and increase its lipogenic potential. SiCOv1 was created by replacing all five lysines in SiCO with arginines. Separately, six cysteine residues within SiCO were deleted to create SiCOv2. SiCOv1 and SiCOv2 mutations were combined to create SiCOv3.Transient expression of SiCOv3 in Nicotiana benthamiana increased TAG by two‐fold relative to SiCO. Constitutive expression of SiCOv3 or SiCOv5, containing the five predominant TAG‐increasing mutations from SiCOv3, in Arabidopsis along with mouse DGAT2 (mD) increased TAG accumulation by 54% in leaves and 13% in seeds compared with control lines coexpressing SiCO and mD.Lipid synthesis rates increased, consistent with an increase in lipid sink strength that sequesters newly synthesized TAG, thereby relieving the constitutive BADC‐dependent inhibition of ACCase reported for WT Arabidopsis. These OLE variants represent novel factors for potentially increasing TAG accumulation in a variety of oil crops. See also the Commentary on this article by Sadre, 243: 7–9. [ABSTRACT FROM AUTHOR]

SUMMARY: Plant triacylglycerols (TAG) are used in food and various industrial feedstocks. LEAFY COTYLEDON 2 (LEC2), a master positive regulator of TAG biosynthesis, regulates a complex network of transcription factors (TFs) during seed development. Aside from WRINKLED1 (WRI1), the TFs regulated by LEC2 related to TAG biosynthesis have not yet been identified. Previously, we identified 25 seed‐expressing TFs that were upregulated in Arabidopsis leaves that overexpressed senescence‐induced LEC2. In this study, each of the 25 TFs was transiently expressed in the leaves of Nicotiana benthamiana to identify unknown TFs that regulate TAG biosynthesis. The TAG content of the transformed leaves was analyzed using thin layer chromatography and gas chromatography. We observed that five TFs, ARABIDOPSIS RESPONSIVE REGULATOR 21 (ARR21), AINTEGUMENTA‐LIKE 6 (AIL6), APETALA2/ETHYLENE RESPONSIVE FACTOR 55 (ERF55), WRKY DNA‐BINDING PROTEIN 8 (WRKY8), and ARABIDOPSIS NAC DOMAIN CONTAINING PROTEIN 38 (ANAC038) increased TAG synthesis in the leaves. Among these, the promoters of AIL6, ERF55, WRKY8, and ANAC038 contain RY motifs, which are LEC2‐binding sites activated by LEC2. AIL6 overexpression in Arabidopsis increased the total fatty acid (FA) content in seeds and altered the FA composition, with increases in 16:0, 18:1, and 18:2 and decreases in 18:0, 18:3, and 20:1 compared with those in the wild type (WT). AIL6 overexpression activates several FA and TAG biosynthesis genes. Therefore, our study successfully identified several new TFs regulated by LEC2 in TAG biosynthesis and showed that AIL6 increased the TAG content in seeds. [ABSTRACT FROM AUTHOR]

Date fruit (Phoenix dactylifera L.) processing generates substantial quantities of date seeds, constituting a potential source of valuable oil yet currently considered waste. This study aimed at characterizing date seed oil (DSO) as a prospective approach for date seed valorization while investigating the influence of geographical origin on its composition. Samples from three Moroccan palm groves (Allougoum, Alnif, and Errachidia) and four non‐Moroccan samples were analyzed. Liquid and gas chromatography were applied to determine the chemical composition. The prevalent fatty acids were oleic acid (Ol), ranging from 40.8% to 50.2%, followed by lauric acid (La, 14.0%–24.2%), myristic acid (My, 9.0%–12.6%), palmitic acid (Pa, 9.0%–11.6%), linoleic acid (7.1%–10.7%), and stearic acid (St, 2.4%–4.8%). Major triacylglycerols identified included LaOlLa, LaOlMy, LaOlPa, LaOlOl, MyOlPa, MyOlOl, and PaOlOl. Notably, DSO contains significant tocochromanol (424–760 mg kg−1) and phytosterol content (3422–4827 mg kg−1), with specific phytosterols identified for the first time. Multivariate analysis employing principal component analysis and hierarchical cluster analysis, supplemented by heatmaps, underscored the influence of geographical origin on DSO composition. Moroccan samples exhibited distinctive profiles rich in St, Ol, Pa, PaOlSt, MyOlPa, PaOlPa, γ‐tocotrienol, and δ‐tocotrienol, leading to a differentiation from non‐Moroccan oils. Challenges in classifying DSO within selected Moroccan palm groves were discussed, emphasizing the necessity to consider major and minor compounds, along with date varieties, in geographical effect studies. Practical Applications: By delving into the chemical properties and potential applications of DSO, this research strives to contribute to sustainable and economically viable approaches for utilizing agricultural waste products. [ABSTRACT FROM AUTHOR]

The Sapindus saponaria (soapberry) kernel is rich in oil that has antibacterial, anti-inflammatory, and antioxidant properties, promotes cell proliferation, cell migration, and stimulates skin wound-healing effects. S. saponaria oil has excellent lubricating properties and is a high-quality raw material for biodiesel and premium lubricants, showing great potential in industrial and medical applications. Metabolite and transcriptome analysis revealed patterns of oil accumulation and composition and differentially expressed genes (DEGs) during seed development. Morphological observations of soapberry fruits at different developmental stages were conducted, and the oil content and fatty acid composition of the kernels were determined. Transcriptome sequencing was performed on kernels at 70, 100, and 130 days after flowering (DAF). The oil content of soapberry kernels was lowest at 60 DAF (5%) and peaked at 130 DAF (31%). Following soapberry fruit-ripening, the primary fatty acids in the kernels were C18:1 (oleic acid) and C18:3 (linolenic acid), accounting for an average proportion of 62% and 18%, respectively. The average contents of unsaturated fatty acids and saturated fatty acids in the kernel were 86% and 14%, respectively. Through the dynamic changes in fatty acid composition and DEGs analysis of soapberry kernels, FATA, KCR1, ECR, FAD2 and FAD3 were identified as candidate genes contributing to a high proportion of C18:1 and C18:3, while DGAT3 emerged as a key candidate gene for TAG biosynthesis. The combined analysis of transcriptome and metabolism unveiled the molecular mechanism of oil accumulation, leading to the creation of a metabolic pathway pattern diagram for oil biosynthesis in S. saponaria kernels. The study of soapberry fruit development, kernel oil accumulation, and the molecular mechanism of oil biosynthesis holds great significance in increasing oil yield and improving oil quality. [ABSTRACT FROM AUTHOR]

Lignocellulosic biomass is currently underutilized, but it offers promise as a resource for the generation of commercial end-products, such as biofuels, detergents, and other oleochemicals. Rhodococcus opacus PD630 is an oleaginous, Gram-positive bacterium with an exceptional ability to utilize recalcitrant aromatic lignin breakdown products to produce lipid molecules such as triacylglycerols (TAGs), which are an important biofuel precursor. Lipid carbon storage molecules accumulate only under growth-limiting low nitrogen conditions, representing a significant challenge toward using bacterial biorefineries for fuel precursor production. In this work, we screened overexpression of 27 native transcriptional regulators for their abilities to improve lipid accumulation under nitrogen-rich conditions, resulting in three strains that accumulate increased lipids, unconstrained by nitrogen availability when grown in phenol or glucose. Transcriptomic analyses revealed that the best strain (#13) enhanced FA production via activation of the β-ketoadipate pathway. Gene deletion experiments confirm that lipid accumulation in nitrogen-replete conditions requires reprogramming of phenylalanine metabolism. By generating mutants decoupling carbon storage from low nitrogen environments, we move closer toward optimizing R. opacus for efficient bioproduction on lignocellulosic biomass. [ABSTRACT FROM AUTHOR]

Background: The herbaceous peony (Paeonia lactiflora Pall.) is extensively cultivated in China due to its root being used as a traditional Chinese medicine known as 'Radix Paeoniae Alba'. In recent years, it has been discovered that its seeds incorporate abundant unsaturated fatty acids, thereby presenting a potential new oilseed plant. Surprisingly, little is known about the full-length transcriptome sequencing of Paeonia lactiflora, limiting research into its gene function and molecular mechanisms. Results: A total of 484,931 Reads of Inserts (ROI) sequences and 1,455,771 full-Length non-chimeric reads (FLNC) sequences were obtained for CDS prediction, TF analysis, SSR analysis and lncRNA identification. In addition, gene function annotation and gene structure analysis were performed. A total of 4905 transcripts were related to lipid metabolism biosynthesis pathway, belonging to 28 enzymes. We use these data to identify 10 oleosin (OLE) and 5 diacylglycerol acyltransferase (DGAT) gene members after de-redundancy. The analysis of physicochemical properties and secondary structure showed them similarity in gene family respectively. The phylogenetic analysis showed that the distribution of OLE and DGAT family members was roughly the same as that of Arabidopsis. Quantitative real-time polymerase chain reaction (qRT–PCR) analyses revealed expression changes in different seed development stages, and showed a trend of increasing and then decreasing. Conclusion: In summary, these results provide new insights into the molecular mechanism of triacylglycerol (TAG) biosynthesis and storage during the seedling stage in Paeonia lactiflora. It provides theoretical references for selecting and breeding oil varieties and understanding the functions of oil storage as well as lipid synthesis related genes in Paeonia lactiflora. [ABSTRACT FROM AUTHOR]

Summary Camelina (Camelina sativa L.), a hexaploid member of the Brassicaceae family, is an emerging oilseed crop being developed to meet the increasing demand for plant oils as biofuel feedstocks. In other Brassicas, high oil content can be associated with a yellow seed phenotype, which is unknown for camelina. We sought to create yellow seed camelina using CRISPR/Cas9 technology to disrupt its Transparent Testa 8 (TT8) transcription factor genes and to evaluate the resulting seed phenotype. We identified three TT8 genes, one in each of the three camelina subgenomes, and obtained independent CsTT8 lines containing frameshift edits. Disruption of TT8 caused seed coat colour to change from brown to yellow reflecting their reduced flavonoid accumulation of up to 44%, and the loss of a well‐organized seed coat mucilage layer. Transcriptomic analysis of CsTT8‐edited seeds revealed significantly increased expression of the lipid‐related transcription factors LEC1, LEC2, FUS3, and WRI1 and their downstream fatty acid synthesis‐related targets. These changes caused metabolic remodelling with increased fatty acid synthesis rates and corresponding increases in total fatty acid (TFA) accumulation from 32.4% to as high as 38.0% of seed weight, and TAG yield by more than 21% without significant changes in starch or protein levels compared to parental line. These data highlight the effectiveness of CRISPR in creating novel enhanced‐oil germplasm in camelina. The resulting lines may directly contribute to future net‐zero carbon energy production or be combined with other traits to produce desired lipid‐derived bioproducts at high yields. [ABSTRACT FROM AUTHOR]

Simple Summary: This review discusses the enzymatic processes governing the initial stages of the synthesis of glycerophospholipids (phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine) and triacylglycerol. The key enzymes analyzed include glycerol-3-phosphate acyltransferases (GPAT) and 1-acylglycerol-3-phosphate acyltransferases (AGPAT). Additionally, because most AGPATs have lysophospholipid acyltransferase (LPLAT) activity, enzymes involved in the Lands cycle with similar functions were also included. The review further explores the potential therapeutic implications of inhibiting these enzymes in the treatment of metabolic diseases and cancer. By elucidating the enzymatic pathways involved in lipid synthesis and their impact on various pathological conditions, the article contributes to the understanding of these processes and their potential as therapeutic targets. This review delves into the enzymatic processes governing the initial stages of glycerophospholipid (phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine) and triacylglycerol synthesis. The key enzymes under scrutiny include GPAT and AGPAT. Additionally, as most AGPATs exhibit LPLAT activity, enzymes participating in the Lands cycle with similar functions are also covered. The review begins by discussing the properties of these enzymes, emphasizing their specificity in enzymatic reactions, notably the incorporation of polyunsaturated fatty acids (PUFAs) such as arachidonic acid and docosahexaenoic acid (DHA) into phospholipids. The paper sheds light on the intricate involvement of these enzymes in various diseases, including obesity, insulin resistance, and cancer. To underscore the relevance of these enzymes in cancer processes, a bioinformatics analysis was conducted. The expression levels of the described enzymes were correlated with the overall survival of patients across 33 different types of cancer using the GEPIA portal. This review further explores the potential therapeutic implications of inhibiting these enzymes in the treatment of metabolic diseases and cancer. By elucidating the intricate enzymatic pathways involved in lipid synthesis and their impact on various pathological conditions, this paper contributes to a comprehensive understanding of these processes and their potential as therapeutic targets. [ABSTRACT FROM AUTHOR]

The crystallization behavior of 1,2,3-tripalmitoyl glycerol or PPP using differential scanning calorimetry (DSC), thermo-optical polarized light microscopy and in situ synchrotron radiation X-ray diffraction (SR-XRD) techniques was precisely examined under static isothermal and dynamic thermal treatments, and the results were compared with preceding studies. PPP was rapidly (15 °C min−1) cooled to target temperatures (from 40 to 59 °C) to determine the precise moment at which crystallization was initiated. Once crystallization ceased, polymorphic transformation and melting were analyzed during subsequent heating. α form was crystallized during isotherms from 40 to 46 °C, temperature at which it coexisted with β′ phase. The latter was solely formed from 47 to 53 °C, and polymorphic crystallization was directed to obtain exclusively most stable β at 54 °C and higher temperatures. Nucleation time values for the α, β′ and β polymorphs exhibited exponential growth type, and a good correlation was found between data obtained by DSC and SR-XRD. Dynamic experiments were based on the use of high (15 °C min−1), intermediate (2 °C min−1) and low (0.5 and 0.1 °C min−1) cooling and heating rates. Thermo-optical polarized light microscopy experiments also provided valuable information on microstructural changes occurring during polymorphic modifications. Less stable forms predominated at high cooling rates, whereas lower velocities leaded the polymorphic crystallization to obtain more stable forms. [ABSTRACT FROM AUTHOR]

Plant oils represent a large group of neutral lipids with important applications in food, feed and oleochemical industries. Most plants accumulate oils in the form of triacylglycerol within seeds and their surrounding tissues, which comprises three fatty acids attached to a glycerol backbone. Different plant species accumulate unique fatty acids in their oils, serving a range of applications in pharmaceuticals and oleochemicals. To enable the production of these distinctive oils, select plant species have adapted specialized oil metabolism pathways, involving differential gene co-expression networks and structurally divergent enzymes/proteins. Here, we summarize some of the recent advances in our understanding of oil biosynthesis in plants. We compare expression patterns of oil metabolism genes from representative species, including Arabidopsis thaliana, Ricinus communis (castor bean), Linum usitatissimum L. (flax) and Elaeis guineensis (oil palm) to showcase the co-expression networks of relevant genes for acyl metabolism. We also review several divergent enzymes/proteins associated with key catalytic steps of unique oil accumulation, including fatty acid desaturases, diacylglycerol acyltransferases and oleosins, highlighting their structural features and preference toward unique lipid substrates. Lastly, we briefly discuss protein interactomes and substrate channeling for oil biosynthesis and the complex regulation of these processes. [ABSTRACT FROM AUTHOR]

Triacylglycerol (TAG) is among the most energy dense storage forms of reduced carbon in living systems. TAG metabolism plays critical roles in cellular energy balance, lipid homeostasis, cell growth and stress responses. In higher plants, microalgae and fungi, TAG is assembled by acyl-CoA-dependent and acyl-CoA-independent pathways catalyzed by diacylglycerol (DAG) acyltransferase and phospholipid:DAG acyltransferase (PDAT), respectively. This review contains a summary of the current understanding of the physiological functions of PDATs. Emphasis is placed on their role in lipid remodeling and lipid homeostasis in response to abiotic stress or perturbations in lipid metabolism. [ABSTRACT FROM AUTHOR]

As climate change intensifies and the need to reduce human-caused emissions becomes more urgent, transitioning to a bio-based economy is essential. This paper explores the diverse industrial applications of plant oils as sustainable alternatives to petroleumbased products, including their use in food, polymers, lubricants, surfactants, pesticides, emollients, and biofuels. This review delves into biosynthetic pathways, detailing the key enzymes and processes involved in the synthesis of triacylglycerol. It thoroughly discusses how genetic and metabolic engineering can not only increase oil yields but also modify fatty acid compositions to better meet industrial requirements. By understanding genetics and utilizing advanced biotechnologies, the oil content and quality of plant sources can be significantly enhanced, aligning with both sustainability goals and industrial demands. This paper provides a comprehensive overview of the current uses and genetic engineering of plant oil production, proposing innovative strategies such as utilizing oils from biomass or cultivating non-edible oil crops. These approaches aim to establish a sustainable industrial system, reduce reliance on fossil fuels, and promote the growth of an environmentally responsible biobased economy. Additionally, the review highlights future directions, examining the economic implications and environmental benefits of adopting plant oils across various sectors and positioning them as pivotal to achieving an eco-friendly, bio-based economy. [ABSTRACT FROM AUTHOR]

Simple Summary: Mealworms are edible insects that are used as food ingredients because nearly all of their edible sections are lipid and protein sources that humans can consume. Triacylglycerols are significant energy sources and the main components of mealworm oil. Due to the composition of triacylglycerol being related in basic properties to oils, the determination of the triacylglycerol composition is important to understand oils. Our study used electrospray ionization tandem mass spectrometry with multiple neutral loss scans to analyze the content and composition of triacylglycerol in mealworm oil. The acquired scan data were deconvoluted to minimize isotopic interference. Additionally, the validation parameters showed relatively good results. The mealworm oil mainly contained triacylglycerol possessing palmitic acid, oleic acid and linoleic acid. The influence of triacylglycerol on mealworm oil is relatively stronger against oxidative stress compared to other vegetable oils. Mealworms (Tenebrio molitor) have been used as an alternative source of proteins and lipids. Triacylglycerols (TAGs) are major sources of energy and have been used to provide essential fatty acids. They are also the main components of mealworm oil, and their composition and content are extensively linked to its physical and chemical properties. However, because of the complexity of TAG molecules, their identification and quantitation are challenging. This study employed electrospray ionization tandem mass spectrometry (ESI-MS/MS) with multiple neutral loss scans (NLS) to analyze the TAG composition and content in mealworm oil. Identifying and quantifying TAGs using ESI-MS/MS in combination with multiple NLS was an efficient way to improve accuracy and timeliness. For the accurate quantification of TAGs, isotopic deconvolution and correlation factors were applied. A total of 57 TAGs were identified and quantified: C52:2 (16:0/18:1/18:1) (1549.4 nmol/g, 18.20%), C52:3 (16:0/18:1/18:2) (1488.1 nmol/g, 17.48%), C54:4 (18:1/18:1/18:2) (870.1 nmol/g, 10.23%), C54:6 (18:1/18:2/18:2) (659.8 nmol/g, 7.76%) and C52:4 (16:0/18:2/18:2) (600.5 nmol/g, 7.06%), which were the most abundant TAGs present in the mealworm oil. The fundamental properties of mealworm oil, including its degree of oxidation, nutritional effect and physical properties, were elucidated. [ABSTRACT FROM AUTHOR]

Mono- and diglycerides play a crucial role in the food industry as multifunctional food additives and emulsifiers. Their importance stems from their unique properties, which allow them to improve the quality, texture, and stability of various food products. Here, results of the kinetic modeling of the mono- and diglycerides synthesis mediated by the lipase Lipozyme® TL 100 L immobilized on the clayey support Spectrogel® type C are reported. The support was characterized by TEM, SEM, and FTIR. Firstly, the influence of pH and lipase load on the immobilization process was analyzed, resulting in an enzymatic activity of 93.2 ± 0.7 U g−1 under optimized conditions (170.9 U g−1 of lipase and pH of 7.1). Afterward, the effects of reaction temperature and concentration of immobilized biocatalyst in the feedstock conversion were evaluated. At optimized parameters, a triglycerides conversion of 97% was obtained at 36.5 °C, 7.9 vol.% of enzyme, a glycerol to feedstock molar ratio of 2:1, and 2 h. The optimized conditions were used to determine the kinetic constants of the elementary reactions involved in the glycerolysis, where a fit superior to 0.99 was achieved between experimental values and predicted data. [ABSTRACT FROM AUTHOR]

Epoxy fatty acid formation during heating was estimated using triolein (OOO) and trilinolein (LLL). Epoxy octadecanoic acids were found in heated OOO, while epoxy octadecenoic acids were found in heated LLL. The content of epoxy fatty acids increased with heating time, and trans-epoxy fatty acids were formed significantly more than cis-epoxy fatty acids. A comparison between OOO and LLL indicated that epoxy fatty acid formation was higher in the OOO than that in the LLL. Heating tests in the presence of α-tocopherol suggested that the formation of epoxy fatty acids could be suppressed by antioxidants. [ABSTRACT FROM AUTHOR]

1,3-Diacylglycerol (DAG) has been well recognized as a safe food component. The metabolic pathway of DAG in the body is different from that of triacylglycerol, and DAG has been demonstrated to contribute to improving metabolism and preventing obesity and cardiovascular diseases. However, the mass fraction of natural DAG in edible oils and fats is less than 10%, so the preparation of high-purity DAG has received widespread attention in recent years. Enzymatic synthesis is the method of choice for the industrial preparation of DAG due to the advantages of eco-friendliness, safety and desirable selectivity over chemical synthesis. Edible livestock and poultry fats, as by-products of slaughter and processing, have a special flavor and nutritional value. However, their undesirable physicochemical properties, especially high melting points and high levels of saturated fatty acids and cholesterols, highly limit their applications in the food industry. In light of this, the synthesis of DAG from edible livestock and poultry fats is a promising approach for their high-value utilization. The current status of the enzymatic synthesis and the potential application of DAG from livestock and poultry fats are reviewed in this article. It is anticipated that this review will provide new ideas for researchers interested in the enzymatic preparation and high-value utilization of DAG from livestock and poultry fats.

Mg2+-independent phosphatidic acid phosphatase (PAP2), diacylglycerol pyrophosphate phosphatase 1 (Dpp1) is a membrane-associated enzyme in Saccharomyces cerevisiae. The enzyme is responsible for inducing the breakdown of β-phosphate from diacylglycerol pyrophosphate (DGPP) into phosphatidate (PA) and then removes the phosphate from PA to give diacylglycerol (DAG). In this study through RNAi suppression, we have demonstrated that Trypanosoma brucei diacylglycerol pyrophosphate phosphatase 1 (TbDpp1) procyclic form production is not required for parasite survival in culture. The steady-state levels of triacylglycerol (TAG), the number of lipid droplets, and the PA content are all maintained constant through the inducible down-regulation of TbDpp1. Furthermore, the localization of C-terminally tagged variants of TbDpp1 in the lysosome was demonstrated by immunofluorescence microscopy. [ABSTRACT FROM AUTHOR]

Background: Physical activity (PA) and/or exercise improves postprandial cardiometabolic risk markers; however, the optimal exercise intensity, frequency, and dose remain unclear. We aimed to (1) compare the acute metabolic effects of interrupted prolonged sitting with PA bouts of different frequencies and durations on blood glucose, insulin, and triacylglycerol responses, and (2) compare the effects of the different types and different times of PA breaks on these measures. Methods: A literature search was carried out using four databases. Network meta-analysis (NMA) and paired meta-analysis were performed to estimate the total standardized mean differences (SMDs) with 95% confidence intervals (95%CI). Results: According to the NMA, compared to prolonged sitting, every 30 min interruption had the highest probability (SUCRA) of being the best intervention for improving blood glucose (SUCRA = 81.8%, SMD = −1.18, 95%CI: −1.72, −0.64) and insulin (SUCRA = 77.5%, SMD = −0.98, 95%CI: −1.36, −0.60). Additionally, every 20 min interruption also significantly lowered blood glucose (SMD = −0.89, 95%CI: −1.52, −0.27) and insulin (SMD = −0.94, 95%CI: −1.41, −0.46). Pairwise meta-analysis suggested that frequent breaks by light-intensity PA significantly lowered glucose (SMD = −1.45, 95%CI: −2.32, −0.57) and insulin (SMD = −1.04, 95%CI: −1.53, −0.55). The same was found for frequent breaks by moderate-to-vigorous PA, which also significantly lowered glucose (SMD = −0.6, 95%CI: −0.83, −0.37) and insulin (SMD = −0.53, 95%CI: −0.73, −0.32). Conclusions: According to the NMA, performing short bouts of PA every 30 min is the most effective prolonged sitting intervention for improving blood glucose and insulin. More evidence is needed to determine the optimal type and time of PA breaks for braking sedentary sitting. PROSPERO Registration: CRD42022340036. [ABSTRACT FROM AUTHOR]

Introduction: Mycobacterium tuberculosis (Mtb), the main cause of tuberculosis (TB), has brought a great burden to the world's public health. With the widespread use of Mtb drug-resistant strains, the pressure on anti-TB treatment is increasing. Anti-TB drugs with novel structures and targets are urgently needed. Previous studies have revealed a series of CYPs with important roles in the survival and metabolism of Mtb. However, there is little research on the structure and function of CYP138. Methods: In our study, to discover the function and targetability of CYP138, a cyp138-knockout strain was built, and the function of CYP138 was speculated by the comparison between cyp138-knockout and wild-type strains through growth curves, growth status under different carbon sources, infection curves, SEM, MIC tests, quantitative proteomics, and lipidomics. Results and discussion: The knockout of cyp138 was proven to affect the Mtb's macrophage infection, antibiotics susceptibility, and the levels of fatty acid metabolism, membrane-related proteins, and lipids such as triacylglycerol. We proposed that CYP138 plays an important role in the synthesis and decomposition of lipids related to the cell membrane structure as a new potential anti-tuberculosis drug target. [ABSTRACT FROM AUTHOR]

Microalgae can convert carbon dioxide into organic matter through photosynthesis. Thus, they are considered as an environment-friendly and efficient cell chassis for biologically active metabolites. Microalgal lipids are a class of organic compounds that can be used as raw materials for food, feed, cosmetics, healthcare products, bioenergy, etc., with tremendous potential for commercialization. In this review, we summarized the commercial lipid products from eukaryotic microalgae, and updated the mechanisms of lipid synthesis in microalgae. Moreover, we reviewed the enhancement of lipids, triglycerides, polyunsaturated fatty acids, pigments, and terpenes in microalgae via environmental induction and/or metabolic engineering in the past five years. Collectively, we provided a comprehensive overview of the products, biosynthesis, induced strategies and genetic engineering in microalgal lipids. Meanwhile, the outlook has been presented for the development of microalgal lipids industries, emphasizing the significance of the accurate analysis of lipid bioactivity, as well as the high-throughput screening of microalgae with specific lipids. [ABSTRACT FROM AUTHOR]

The oleaginous yeast, Lipomyces starkeyi can have diverse industrial applications due to its remarkable capacity to use various carbon sources for the biosynthesis intracellular triacylglycerides (TAGs). In L. starkeyi , TAG synthesis is enhanced through upregulation of genes involved in citrate-mediated acyl-CoA synthesis and Kennedy pathways through the transcriptional regulator LsSpt23p. High expression of LsSPT23 can considerably enhance TAG production. Altering the regulatory factors associated with lipid production can substantially augment lipid productivity. In this study, we identified and examined the L. starkeyi homolog sucrose nonfermenting 1 SNF1 (LsSNF1) of YlSNF1 , which encodes a negative regulator of lipid biosynthesis in the oleaginous yeast Yarrowia lipolytica. The deletion of LsSNF1 enhanced TAG productivity in L. starkeyi , suggesting that LsSnf1p is a negative regulator in TAG production. The enhancement of TAG production following deletion of LsSNF1 can primarily be attributed to the upregulation of genes in the citrate-mediated acyl-CoA synthesis and Kennedy pathways, pivotal routes in TAG biosynthesis. The overexpression of LsSPT23 enhanced lipid productivity; strain overexpressing LsSPT23 and without LsSNF1 exhibited increased TAG production capacity per cell. LsSnf1p also has a significant role in the utilization of carbon sources, including xylose or glycerol, in L. starkeyi. Our study results elucidated the role of LsSnf1p in the negative regulation of TAG synthesis in L. starkeyi , which has not previously been reported. • Lipomyces starkeyi SNF1 homolog (LsSNF1) was identified and the LsSNF1 deletion mutant was constructed. • LsSnf1p had important roles in lipid production and carbon-source utilization in L. starkeyi. • The overexpression of LsSpt23p and without LsSnf1p exhibited increased TAG production capacity per cell. [ABSTRACT FROM AUTHOR]

With the increasing impacts of climate change, establishing more sustainable and robust plants such as desert dates (Balanites aegyptiaca) seems to be necessary. Known for its resilience in arid conditions, this tree has the potential to become a more important food source, particularly for its potential to yield edible oil. This study characterized Balanites kernel oil (BKO) as a promising oil source in arid regions, studying the influence of geographical origin and environmental factors. Moroccan and Sudanese BKO samples were analyzed and compared with Mauritanian BKO. In the fatty acid profile, unsaturated fatty acids constituted over 70% of the BKO profile, with a predominance of linoleic acid (Li), oleic acid (Ol), palmitic acid (Pa), and stearic acid (St). Consequently, the predominant triacylglycerols were PaLiLi, PaLiOl, LiLiOl, OlLiOl, and StLiOl. α-Tocopherol dominated the tocochromanol composition (324 to 607 mg/kg), followed by γ-tocopherol (120 to 226 mg/kg), constituting 90% of the total tocochromanols. The total phytosterol content in BKO ranged from 871 to 2218 mg/kg oil, with β-sitosterol dominating (58% to 74%). Principal Component Analysis revealed that the geographical origin significantly influences BKO composition, emphasizing environmental factors, particularly water deficit and/or temperatures. Notably, Moroccan BKO collected from an area characterized by high aridity and relatively low winter temperatures, showcased a unique profile in fatty acid, phytosterols, and tocochromanols. The valorization of BKO presents an opportunity for local agricultural development in arid regions and a role model for plant development and agricultural practices in other parts of the world. [ABSTRACT FROM AUTHOR]

Vegetables including asparagus contain a wide range of fatty acids, mainly stored in triacylglycerols. One class of interesting minor fatty acids is the family of furan fatty acids (FuFAs) because of their antioxidant properties. Since FuFAs have not been studied previously in asparagus (Asparagus officinalis L.), we developed and applied a simplified method for their analysis in 20 fresh and three preserved samples. Four FuFAs were detected with clear dominance of the dimethyl‐substituted FuFAs (D‐FuFAs) 11D5 and 9D5 as well as small amounts of the monomethyl‐substituted FuFAs (M‐FuFAs) 11M5 and 9M5. The total amounts of FuFAs in fresh white asparagus ranged from 1.4 to 4.6 mg/100 g dry weight (mean 3.0 mg/100 g dry weight). Subsequent LC‐Q‐Orbitrap‐HRMS measurements enabled the detection of 22 different FuFA‐containing TAGs. These were predominantly found together with one or two polyunsaturated fatty acid. [ABSTRACT FROM AUTHOR]

Although many studies have examined the biochemical metabolic pathways by which an egg (egg yolk) lowers blood lipid levels, data on the molecular biological mechanisms that regulate and induce the partitioning of hepatic glycerolipids are missing. The aim of this study was to investigate in vivo monitoring in four study groups using an animal nutrition biomodel fitted with a jugular-vein cannula after egg yolk intake: CON (control group, oral administration of 1.0 g of saline), T1 (oral administration of 1.0 g of pork belly fat), T2 (oral administration of 1.0 g of smart-farm egg yolk), and T3 (oral administration of T1 and T2 alternately every week). The eggs induced significant and reciprocal changes in incorporating 14C lipids into the total glycerolipids and releasing 14CO2, thereby regulating esterification and accelerating oxidation in vivo. The eggs increased phospholipid secretion from the liver into the blood and decreased triacylglycerol secretion by regulating the multiple cleavage of fatty acyl-CoA moieties' fluxes. In conclusion, the results of the current study reveal the novel fact that eggs can lower blood lipids by lowering triacylglycerol secretion in the biochemical metabolic pathway of hepatic glycerolipid partitioning while simultaneously increasing phospholipid secretion and 14CO2 emission. [ABSTRACT FROM AUTHOR]

Seven mango seeds of Ivorian varieties, that is, Amelie, Kent, Keitt, Brooks, Palmer, Dadiani, and Djakoumankoun were collected from Northern Ivory Coast as wastes from local processing factories and local markets; their kernels were then quickly separated and sun‐dried to ensure lipases inactivation. This study was carried out to elucidate (1) the variability in the proximate composition (protein, ash, and fat content) of the so‐obtained kernels and (2) the characteristics of the extracted fat including acidity, peroxide value, unsaponifiable matters, phospholipid content, iodine value, fatty acid (FA), and triacylglycerol (TAG) composition, sterol content, oxidative stability, color, and carotenoid content. It was found that the fat content ranged from 4.9% to 9.6% (dry matter) depending on the variety. Whatever the variety, oleic (O, 35.9%–47%) and stearic acid (St, 30.3%–48.3%) were the most abundant FA. A wide range of variation in TAG composition was however observed: the major were StOSt (23.9%–45.9%), StOO (15.5%–25.8%), and StLSt (10.4%–12.5%). A classification of the seven varieties based on their FA, TAG, and sterol content, was established using multivariate analysis: principal component analysis (PCA) and cluster analysis (CA). According to their main FA and TAG, the seven varieties may be grouped into four clusters: cluster I = Amelie, cluster II = Keitt, Palmer, and Kent, cluster III = Brooks and Dadiani and, cluster IV = Djakoumankoun. [ABSTRACT FROM AUTHOR]

Summary: Lysophosphatidic acid acyltransferases (LPAATs) catalyze the formation of phosphatidic acid (PA), a central metabolite in both prokaryotic and eukaryotic organisms for glycerolipid biosynthesis. Phaeodactylum tricornutum contains at least two plastid‐localized LPAATs (ptATS2a and ptATS2b), but their roles in lipid synthesis remain unknown.Both ptATS2a and ptATS2b could complement the high temperature sensitivity of the bacterial plsC mutant deficient in LPAAT. In vitro enzyme assays showed that they prefer lysophosphatidic acid over other lysophospholipids.ptATS2a is localized in the plastid inner envelope membrane and CRISPR/Cas9‐generated ptATS2a mutants showed compromised cell growth, significantly changed plastid and extra‐plastidial membrane lipids at nitrogen‐replete condition and reduced triacylglycerols (TAGs) under nitrogen‐depleted condition. ptATS2b is localized in thylakoid membranes and its knockout led to reduced growth rate and TAG content but slightly altered molecular composition of membrane lipids.The changes in glycerolipid profiles are consistent with the role of both LPAATs in the sn‐2 acylation of sn‐1‐acyl‐glycerol‐3‐phosphate substrates harboring 20:5 at the sn‐1 position. Our findings suggest that both LPAATs are important for membrane lipids and TAG biosynthesis in P. tricornutum and further highlight that 20:5‐Lyso‐PA is likely involved in the massive import of 20:5 back to the plastid to feed plastid glycerolipid syntheses. [ABSTRACT FROM AUTHOR]

The main objective of this study was to evaluate the quality and select the best varieties from 85 olive varieties in the Liangshan region, China, from the perspective of oil content, fatty acid, and triacylglycerol compositions. Although 85 varieties of olive oil showed no difference in the type of fatty acid composition and the distribution of triglycerides, they varied greatly in the oil content, the relative proportion of fatty acids, and triglycerides. Principal component analysis (PCA) and cluster and heatmap analysis clearly divided the 85 samples into three groups. Group A included 36 varieties and was characterized by high oleic acid, mono-unsaturated fatty acids (MUFA), eicosenoic acid, OOO (glyceryl trioleate), and OOL (1-oleic acid-2-oleic acid-3-linoleic acid glycerides) contents, but rare palmitoleic acid, SFA (saturated fatty acid), and POP (1-palmitic acid-2-oleic acid-3-palmitic acid glycerides) levels. Group B contained 9 varieties of olive, with the main characteristics of high oleic acid, linolenic acid, MUFA, MUFA/PUFA, SFA, and OLnO (1-oleic acid-2-linolenic acid-3-oleic acid glycerides) content, but low in linoleic acid, PUFA (polyunsaturated fatty acid), PLL levels and ratio of omega-6 to omega-3 polyunsaturated fatty acids. Group C comprised 40 varieties, identified by high linoleic acid and PUFA levels but low ratios of oleic acid, MUFA, OOO, MUFA/PUFA, and the ratio of omega-6 to omega-3 polyunsaturated fatty acids. Combined with the oil content (FW) (%) of 85 varieties, we suggested four distinguished varieties in Group A, namely Lechín, Coratina, Koroneiki, and Arbosana; three distinguished varieties in Group B, namely Picual, Ezhi, Cornicabra; two distinguished varieties in Group C namely Frantoio and Arbequina as the objects of large-scale cultivation by Chinese cultivators. More importantly, the obtained data also demonstrated that some locally bred specific varieties, such as Zhongze-3 and Yuntai, had interesting unsaturated fatty acids and had cultivation and popularization value in China. [ABSTRACT FROM AUTHOR]

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