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Assisted reproductive technologies (ARTs), such as artificial insemination, semen sorting and freezing, embryo production in vivo and in vitro, are all methods in which animal reproduction management of several species has become considerably more efficient. These ARTs have been applied both in domestic and wild ruminants. In this study, in vitro embryo production was attempted with oocytes collected surgically during the mating season, from live white-tailed deer (WTD) hinds, maintained under captivity, in Northeast México. The study was conducted in two WTD farms nearby Cd. Victoria, Tamps., México, and another WTD farm located in Guerrero Coahuila, México. The laboratory work was carried at the Centro de Desarrollo de la Capacidad Productiva y Mejoramiento Genético de la Ganadería, property of the Unión Ganadera Regional de Tamaulipas (Centro-UGRT). The deer hinds are kept in captivity year-around, with feeding and health management provided accordingly to their requirements (50-60 kg females). Fresh green forage and clean fresh water is supplied daily. Oocytes were collected on each farm, while the hinds were maintained under general anesthesia and by means of a mid-ventral laparotomy, ovaries were exposed and follicles greater than 1-2 mm were aspirated with a 20 G needle, connected by a two-way plastic and latex hose to a vacum machine (WTA, Brazil); the oocytes were collected into a 50 ml. centrifuge tube containing wash media with heparine (Vitrogen, Brazil). Each oocyte collection lasted approximately 20 minutes, after which and while still on the farm, the oocytes were filtered with 50 µ mesh filters and rinsed several times with wash media, then placed on search Petri plates. Oocytes were then counted and classified (total, viable and non-viable oocytes) and placed into cryovials with maturation media (3 ml. MIV, Vitrogen, Brazil) and placed into a portable incubator with 5 % CO2 gas mix (LabiMixWTA, Brazil), after all hinds were done, oocytes were transported to the in vitro fertilization laboratory (IVF Lab) at the Centro-UGRT. Once at the IVF Lab, the oocytes were placed on a larger incubator (Eve, WTA, Brazil) and kept there for 18-20 hours, after which, the MIV media was changed for fertilization media (FIV, Vitrogen, Brazil) and fertilization was initiated by adding 10,000-12,000 live sperm per cryovial, and incubated for an additional period of 24 hours; after which, FIV media was replaced by the same media and at this point, cleavage rate was estimated by counting the oocytes that initiated cell división. At 72 hours after cell division started, fertilization rate was estimated; and 7 days after, the blastocysts were counted and classified. The whole process of oocyte maturation and embryo production in vitro, was conducted based on a beef cattle embryo production system and adapted to a deer embryo production system using media for small ruminants (Vitrogen, Brazil). Data collected per hind included total number of oocytes, viable and non-viable oocytes, cleavage rate (ratio of viable oocytes that initiated cell division over viable oocytes), fertilization rate (ratio of embryos that initiated cleavage over those that continued development to the blastocyst stage) and blastocyst rate (embryos reaching the blastocyst stage over cleaved embryos); averages were calculted for each parameter. The main results from this study on a per hind basis for total viable and non-viable oocytes were 9.8, 6.3 and 4.5, respectively; cleavage and blastocyst rates were 39.5 and 36.8, respectively and 2.3 blastocysts. In conclusión, oocyte collection from live WTD hinds and in vitro embryo production was succesfully done under farming conditions in Northeast México.

El objetivo de la presente investigación es identificar la influencia del aprendizaje organizacional (AO) en el desempeño organizacional (DO) de pequeñas y medianas empresas del sector comercial en Ciudad Obregón, Sonora; México. Mediante una investigación cuantitativa con un diseño no experimental transeccional, se aplicó un cuestionario con un total de 43 ítems a 136 empresas. Los resultados muestran que existe una influencia positiva y significativa del AO en el DO de PyMES comerciales. Se concluye que al interior de las organizaciones estudiadas se presenta un proceso de adquisición, distribución e intercambio de conocimiento mediante el aprendizaje e incluso este suele pasar a formar parte de la misma como memoria organizacional.

CIES2020 - XVII Congresso Ibérico e XIII Congresso Ibero-americano de Energia Solar RESUMEN: La disponibilidad de herramientas que permitan realizar estudios de viabilidad en proyectos de energía solar térmica para procesos industriales (SHIP), de manera rápida, precisa y con bajo coste, es crítica para la aceptación de esta tecnología por el sector industrial. Las herramientas surgidas en respuesta a esta demanda son muy heterogéneas, lo que dificulta la comparación de proyectos. Esta disparidad limita el desarrollo comercial de la tecnología al generar incertidumbre en empresas promotoras y entidades financieras. En este artículo se presenta una metodología para unificar los esfuerzos que se están llevando a cabo para el desarrollo de estas herramientas de simulación. La metodología propuesta combina la utilización de código abierto y privado. De esta forma se garantiza un desarrollo conjunto y continuado por universidades y centros público/privados, además de permitir a empresas privadas su utilización, manteniendo privada cualquier información sensible. ABSTRACT: The availability of tools capable of performing pre-feasibility studies of Solar Heat for Industrial Processes (SHIP) projects in a fast, precise, and low cost way has become necessary for the acceptance of this technology by the industrial sector. The tools that have emerged so far are very heterogeneous, making it difficult to benchmark projects. The significant disparity in the toold limits the commercial development of the technology by generating uncertainty in project promoters and financial entities. This work presents a methodology to unify the efforts that are being carried out for the development of these simulation tools. The proposed methodology combines the use of open and proprietary source. In this way, open source development is maintained by universities and public / private centers, while private companies still keep any sensitive information private. info:eu-repo/semantics/publishedVersion

© 2017 The Authors. Carbon monoxide-releasing molecules (CORMs) are a promising class of new antimicrobials, with multiple modes of action that are distinct from those of standard antibiotics. The relentless increase in antimicrobial resistance, exacerbated by a lack of new antibiotics, necessitates a better understanding of how such novel agents act and might be used synergistically with established antibiotics. This work aimed to understand the mechanism(s) underlying synergy between a manganese-based photoactivated carbon monoxide-releasing molecule (PhotoCORM), [Mn(CO) 3 (tpa-κ 3 N)]Br [tpa=tris(2-pyridylmethyl)amine] , and various classes of antibiotics in their activities towards Escherichia coli EC958, a multi-drug-resistant uropathogen. The title compound acts synergistically with polymyxins [polymyxin B and colistin (polymyxin E)] by damaging the bacterial cytoplasmic membrane. [Mn(CO) 3 (tpa-κ 3 N)]Br also potentiates the action of doxycycline, resulting in reduced expression of tetA, which encodes a tetracycline efflux pump. We show that, like tetracyclines, the breakdown products of [Mn(CO) 3 (tpa-κ 3 N)]Br activation chelate iron and trigger an iron starvation response, which we propose to be a further basis for the synergies observed. Conversely, media supplemented with excess iron abrogated the inhibition of growth by doxycycline and the title compound. In conclusion, multiple factors contribute to the ability of this PhotoCORM to increase the efficacy of antibiotics in the polymyxin and tetracycline families. We propose that light-activated carbon monoxide release is not the sole basis of the antimicrobial activities of [Mn(CO) 3 (tpa-κ 3 N)]Br.