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Abstract The medical device and pharmaceutical industries include a range of drugs, machines, instruments, and apparatuses used to prevent, diagnose, treat disease and illness, or aid in rehabilitation for patients, and are expected to grow substantially in the coming years. However, they are often targets of criminal organizations who manufacture and profit from fraudulent products, infiltrating the market with counterfeit medical supply chains. In this paper, we discuss and analyze the extent and nature of this problem and make suggestions for mitigation and prevention of this worldwide challenge. Ultimately, we argue that a holistic approach is essential to addressing this problem, including the creation and dissemination of reliable and good quality data, developing healthcare systems to be more robust, establishing/enhancing intra‐ and international cooperation around this issue, and employing effective technological solutions, such as digital tracing.

Hyperspectral measurements can help with rapid decision-making and collecting data across multiple locations. However, there are multiple data processing methods (Savisky-Golay [SG], first derivative [FD], and normalization) and analyses (partial least squares regression [PLS], weighted k-nearest neighbor [KKNN], support vector machine [SVM], and random forest [RF]) that can be used to determine the best relationship between physical measurements and hyperspectral data. In the current study, FD was the best method for data processing and SVM was the best model for predicting average cotton (Gossypium spp. Malvaceae) height and nodes. However, the combination of FD and RF were best at predicting cotton leaf area index, canopy cover, and chlorophyll content across the growing season. Additionally, results from models developed by both SVM and RF were closely related to pseudo-CHIME satellite wavebands, where in-situ hyperspectral data were matched to the spectral resolutions of a future hyperspectral satellite. The information and results presented will aid producers and other members of the cotton industry to make rapid and meaningful decisions that could result in greater yield and sustainable intensification.

Introduction Multimodal pain control following cardiothoracic surgery remains a focus in international guidelines. We hypothesise that non-depolarising skeletal muscle relaxants can prove to be a useful adjunct for this population.Methods/analysis This systematic review will focus on human adult studies of pain control using muscle relaxants within 1 week following cardiac and thoracic surgery available in PubMed, Cochrane Central, Web of Science and EMBASE. Target studies will have a primary focus on measured effects on quality of pain control and reduction in opioid usage. Studies that include non-depolarising skeletal muscle relaxants given during cardiothoracic surgery or in the week after will be included. Study selection will be in keeping with Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Procedures and agents used will be analysed together, and a meta-analysis will be conducted then compared with current therapies recommended in international practice guidelines.Ethics and dissemination Formal ethical approval will not be required as primary data will not be collected. The results will be disseminated through peer-reviewed publication, conference presentation and lay press.PROSPERO registration number CRD42023397917.

Introduction: The purpose of this study was to capture experiences and explore perceptions of clinical faculty research participation at an academic medical center in the southeastern United States to allow leadership to train, recruit, and retain faculty members and increase research production by better understanding motivations for and barriers to academic research participation. A secondary objective was to gauge the impact of coronavirus disease 2019 (COVID-19) on clinical faculty and their research experiences. Methods: This explanatory sequential mixed methods study was conducted in two phases. In Phase I, a quantitative questionnaire was distributed to 482 individuals across 18 of the academic medical center’s 23 School of Medicine departments. Data were analyzed to determine if research experiences differed based on research participation, gender, tenure status, early career investigator status, and faculty rank. In Phase II, qualitative in-person interviews were conducted using a semi-structured interview guide. Results: In total, 100 Phase I questionnaires were completed, 93 of which met study inclusion criteria. Sixteen Phase I participants opted-in to Phase II interviews, and 11 interviews were successfully completed. Results from Phases I and II indicated several important emerging themes, leading to the creation of three overarching study categories: barriers to clinical research, divide in research training and education, and the impact of COVID-19. Discussion: Results indicated several important emerging themes related to clinical research experiences and perceptions at an academic medical center. These findings will provide leadership with important insight into the experiences and perceptions of clinical faculty and their research participation at the academic medical center, including their motivations for and barriers to research participation.

Abstract Tepary bean (Phaseolus acutifolius A. Gray) is an underutilized drought tolerant annual legume, originating from the Sonoran Desert, that may be a beneficial forage/hay for beef cattle in the Southern Great Plains of the US (SGP). The SGP has erratic rainfall and periods of intermittent drought exacerbated by high summer temperatures. In 2020 and 2021, a split-plot design was used to evaluate 13 genotypes of tepary bean and a forage soybean (control) at El Reno, OK, USA to compare production of plant biomass and forage nutritive value parameters under seven harvest regimes. Genotypes were used as the main plot and cutting management as the sub-plot. Biomass production of all tepary bean genotypes equaled that of soybean (p > 0.05), while several genotypes had superior forage nutritive value traits (p ≤ 0.05). Overall, a 15-cm cutting height and 30-day harvest interval produced the best overall product (average dry biomass of 5.8 Mg ha−1 with average relative feed values (RFV) of 165). Although all harvest regimes reduced total seasonal biomass, forage nutritive value increased. However, the tradeoff between forage production and nutritive value may be unacceptable to most producers. Further agronomic and breeding research is needed to encourage producers to grow tepary bean as a forage/hay in the SGP.

There is a significant unmet need for clinical reflex tests that increase the specificity of prostate-specific antigen blood testing, the longstanding but imperfect tool for prostate cancer diagnosis. Towards this endpoint, we present the results from a discovery study that identifies new prostate-specific antigen reflex markers in a large-scale patient serum cohort using differentiating technologies for deep proteomic interrogation. We detect known prostate cancer blood markers as well as novel candidates. Through bioinformatic pathway enrichment and network analysis, we reveal associations of differentially abundant proteins with cytoskeletal, metabolic, and ribosomal activities, all of which have been previously associated with prostate cancer progression. Additionally, optimized machine learning classifier analysis reveals proteomic signatures capable of detecting the disease prior to biopsy, performing on par with an accepted clinical risk calculator benchmark.

FLT3L-Fc is a half-life extended, effectorless Fc-fusion of the native human FLT3-ligand. In cynomolgus monkeys, treatment with FLT3L-Fc leads to a complex pharmacokinetic/pharmacodynamic (PK/PD) relationship, with observed nonlinear PK and expansion of different immune cell types across different dose levels. A minimal physiologically based PK/PD model with expansion-enhanced target-mediated drug disposition (TMDD) was developed to integrate the molecule’s mechanism of action, as well as the complex preclinical and clinical PK/PD data, to support the preclinical-to-clinical translation of FLT3L-Fc. In addition to the preclinical PK data of FLT3L-Fc in cynomolgus monkeys, clinical PK and PD data from other FLT3-agonist molecules (GS-3583 and CDX-301) were used to inform the model and project the expansion profiles of conventional DC1s (cDC1s) and total DCs in peripheral blood. This work constitutes an essential part of our model-informed drug development (MIDD) strategy for clinical development of FLT3L-Fc by projecting PK/PD in healthy volunteers, determining the first-in-human (FIH) dose, and informing the efficacious dose in clinical settings. Model-generated results were incorporated in regulatory filings to support the rationale for the FIH dose selection.

While the sentinel nature of freshwater systems is now well recognized, widespread integration of freshwater processes and patterns into our understanding of broader climate-driven Arctic terrestrial ecosystem change has been slow. We review the current understanding across Arctic freshwater systems of key sentinel responses to climate, which are attributes of these systems with demonstrated and sensitive responses to climate forcing. These include ice regimes, temperature and thermal structure, river baseflow, lake area and water level, permafrost-derived dissolved ions and nutrients, carbon mobilization (dissolved organic carbon, greenhouse gases, and radiocarbon), dissolved oxygen concentrations, lake trophic state, various aquatic organisms and their traits, and invasive species. For each sentinel, our objectives are to clarify linkages to climate, describe key insights already gained, and provide suggestions for future research based on current knowledge gaps. We suggest that tracking key responses in Arctic freshwater systems will expand understanding of the breadth and depth of climate-driven Arctic ecosystem changes, provide early indicators of looming, broader changes across the landscape, and improve protection of freshwater biodiversity and resources.

Summary: Single-cell whole-genome sequencing (scWGS) enables the assessment of genome-level molecular differences between individual cells with particular relevance to genetically diverse systems like solid tumors. The application of scWGS was limited due to a dearth of accessible platforms capable of producing high-throughput profiles. We present a technique that leverages nucleosome disruption methodologies with the widely adopted 10× Genomics ATAC-seq workflow to produce scWGS profiles for high-throughput copy-number analysis without new equipment or custom reagents. We further demonstrate the use of commercially available indexed transposase complexes from ScaleBio for sample multiplexing, reducing the per-sample preparation costs. Finally, we demonstrate that sequential indexed tagmentation with an intervening nucleosome disruption step allows for the generation of both ATAC and WGS data from the same cell, producing comparable data to the unimodal assays. By exclusively utilizing accessible commercial reagents, we anticipate that these scWGS and scWGS+ATAC methods can be broadly adopted by the research community. Motivation: Single-cell whole-genome sequencing (scWGS) is a powerful tool for studying complex, heterogeneous biology. The motivation for this study was to establish an scWGS method that relies only on commercially available reagents and instrumentation to make the technology accessible to the widest possible segment of the research community.

Abstract Deep-sea mining of hydrothermal deposits off the coast of Japan is currently under consideration, and environmental baseline studies of the area are required to understand possible impacts. The aim of this study is to clarify population structures of dominant benthic megafaunal species near hydrothermal vent fields in the Okinawa Trough, using a population genetics approach. We examined dominant deep-sea scavenging species including eels, several amphipods, and a decapod and performed population genetic analyses based on the mitochondrial cytochrome c oxidase subunit I region. Several sites were sampled within Okinawa Trough to examine intra-population diversity while two other locations 1400–2400 km away were chosen for inter-population comparisons. For synaphobranchid eels Simenchelys parasitica and Synaphobranchus kaupii, our results showed significant intra-population diversity but no inter-population genetic differentiation, suggesting strong genetic connectivity and/or large population sizes. In addition, single nucleotide polymorphism analysis also confirmed strong genetic connectivity for Simenchelys parasitica. Among scavenging amphipods, we detected seven putative species using molecular phylogenetic analysis. We evaluated population structures of the most abundant species of amphipods and a decapod species (Nematocarcinus lanceopes). Our results provide basic information on the genetic population structures of benthic megafaunal species near hydrothermal vent fields, which can be used to select candidate species for future connectivity analysis with high-resolution genetic markers and aid understanding of the potential population impacts of environmental disturbances.

Historically, the Y chromosome has presented challenges to classical methodology and philosophy of understanding the differences between males and females. A genetic unsolved puzzle, the Y chromosome was the last chromosome to be fully sequenced. With the advent of the Human Genome Project came a realization that the human genome is more than just genes encoding proteins, and an entire universe of RNA was discovered. This dark matter of biology and the black box surrounding the Y chromosome have collided over the last few years, as increasing numbers of non-coding RNAs have been identified across the length of the Y chromosome, many of which have played significant roles in disease. In this review, we will uncover what is known about the connections between the Y chromosome and the non-coding RNA universe that originates from it, particularly as it relates to long non-coding RNAs, microRNAs and circular RNAs.

Utilizing insects in legal investigations as a tool for estimating forensically important timelines (e.g., minimum post-mortem interval (min-PMI)) is becoming more commonly used and accepted throughout the world. In the United States much of the climate is temperate, however, the Sonoran Desert is an arid location with extreme heat and irregular rainfall. Work on forensically relevant insects in this region is severely understudied. This study surveyed the populations of forensically important blow flies (Diptera: Calliphoridae) in Phoenix, Arizona for one year using traps baited with four different food sources. Nine species across four genera were collected with Lucilia sericata, Calliphora latifrons, and Lucilia mexicana accounting for 98.6% of total blow flies captured in the baited traps. Abundance drastically changed throughout the year, ranging from 500+ flies to 0 flies captured in a month, with species abundance correlating with temperature and humidity. These results reveal that environmental conditions (i.e., maximum temperature and relative humidity) may limit blow fly activity or seasonally remove (or make inactive) entire local populations, thus affecting their ability to colonize remains and produce an accurate min-PMI.

A 65-year-old male was admitted for cardiogenic shock (ejection fraction: 15%) with severe aortic stenosis and regurgitation. He underwent emergency angiography and mechanical circulatory support. A multidisciplinary heart team discussed treatment options. Ultimately, he underwent successful emergency surgical aortic valve replacement with recovery on extracorporeal membrane oxygenation. (Level of Difficulty: Intermediate.)

Castor (Ricinus communis L.) is an important industrial crop with a wide range of industrial and pharmaceutical applications. Brazil is among the largest castor-producing countries. Between 2004 and 2010, castor cultivation was stimulated with an emphasis towards biodiesel production. However, this was not enough to leverage the production of castor in Brazil, mainly due to the lack of structured trade and the competition with other cheaper raw materials for the production of biodiesel. Despite this failure, the species presents itself as an excellent alternative for crop rotation in the second crop among soybean, corn, beans, and cotton cultivation areas as the oil is highly valuable for other products. Moreover, it has drawn the attention of producers and researchers in Brazil for this potential rotation as it is considered a plant tolerant of water-deficiency and is highly susceptible to gray mold, a disease favored by high humidity in the final stages of the crop. For instance, its cultivation in the second crop in Cerrado regions, where rains occur in the early stages of the crop and cease when the plants reach the final stage of production, has been successful and shows great promise. The current study aimed to evaluate the suitability of environments throughout Brazil to grow castor, incorporating variables associated with the incidence of gray mold and confirm these findings based on existing castor trial data obtained from the literature. The site suitability analysis determined that 74.99 million hectares - 8.8% of Brazilian territory - are highly suitable for castor production during second harvest, mostly located in the Northeastern and Midwestern regions. These results are surprising since Brazil currently has around 7.8% (∼66.81 million hectares) of its territory occupied with agriculture (grains, fruits, vegetables, and perennial crops). The findings of this study provide a method to perform site suitability for crops using data associated with agronomic and disease characteristics, as is the case with gray mold that often results in significant losses in castor production. Also, this analysis provides evidence for the great potential of Brazil to increase castor production and meet the world demand for its oil through utilization of second-crop cultivation.

Integrated pest management (IPM) is an important component of golf course maintenance and includes conventional chemical pesticide use as well as nonchemical cultural management practices. Determining how frequent pest management practices are used on golf courses is critical when developing educational and outreach programs. The objective of this study was to determine the frequency of pest management practices and pesticide mixing and storage facilities on US golf courses. A survey was sent to 14,033 operational US golf facilities with 10% responding. Reliance on all conventional chemical pesticides increased from 2015 to 2021. The reliance on biological control products declined to 14% and reliance on the nonpesticide practice of using plant growth regulators remained equivalent to 2015. The most common pest management practices included monitoring weather patterns and scouting for pests, with 93% of golf facilities reporting the use of both. The use of written IPM and pesticide application plans increased from 44% to 63% of golf facilities between 2015 and 2021, respectively. Generally, mixing and storage facilities remained unchanged from 2015 to 2021. US golf facilities continue to use nonchemical pest management practices, but reliance on chemical pesticides has increased.

Nutrient use on United States golf courses increases management costs and has the potential to influence ecosystems. Therefore, it is critical to assess nutrient use and management practices to develop and teach best management practices. The objectives of this survey were to measure nutrient use and management practices on United States golf courses in 2021, and to determine if changes occurred since 2006. A survey was developed and distributed via e-mail to 14,033 United States golf facilities, with 1444 responding. From 2006 to 2021, the total projected nitrogen (N), available phosphorus (P2O5), and soluble potash (K2O) applied declined by 41%, 59%, and 54%, to 54,376, 13,761, and 41,386 tons, respectively. These reductions were attributed to course closures, reduced fertilized acres, reduced application rates, and nutrient use restrictions. The percentage of facilities that did not apply P2O5 increased to 21%, which is likely a result of P2O5 application restrictions. Soil testing was associated with greater application rates of N, P2O5, and K2O. Returning clippings, using precision fertilizer applications, reducing turfgrass acreage, and considering N release from soil organic matter were associated with reduced application rates of P2O5. Golf course superintendents have contributed to nationwide reductions in N, P2O5, and K2O, as evidenced by the reduction in fertilized acres and the reduction in nutrient use rates from 2006 to 2021.

The family Polychrotidae Fitzinger, 1843 is represented in Honduras by a single species, Polychrus gutturosus Berthold, 1845. This canopy dweller is distributed in the lowland moist forests and riparian forest within pine savannah of the northern and eastern regions of Honduras. Only 13 records and nine localities support the presence of the species in the country; therefore, the distribution and natural history of the species in Honduras is essentially unknown. Herein, we provide a new locality and natural history observations of a specimen captured in the core zone of the Reserva del Hombre y La Biosfera del Río Plátano. This record represents the north-western most occurrence of Polychrus gutturosus in a region known as the Mosquitia and fills an important distributional gap for this poorly known species.

Golf facilities account for 2.3 million acres in the United States. Numerous turfgrass species are managed on US golf facilities, but golf facilities may change turfgrasses depending on numerous variables. Knowing which turfgrasses are grown and how turfgrass selection has changed would provide important information to scientists, turfgrass managers, and policymakers. The objective of this survey was to measure turfgrass use on US golf facilities in 2021 and to determine whether changes in turfgrass selection have occurred since 2005. A survey was developed and distributed via e-mail to 13,938 US golf facilities, with 1861 responding. From 2005 to 2021, the total projected area of maintained turfgrass on US golf facilities decreased by 14.2%, which was likely a result of course closures and maintenance operations. Nationally, bermudagrass (Cynodon sp.) and Kentucky bluegrass (Poa pratensis) remained the most common warm- and cool-season turfgrasses, respectively. The area of winter-overseeded turfgrass declined by 60% between 2005 and 2021. The percentage of golf facilities that used zoysiagrass (Zoysia sp.) and seashore paspalum (Paspalum vaginatum) increased depending on region and specific playing surface, albeit a pragmatically minor increase. In general, turfgrass selection on golf facilities in northern climates did not change, whereas turfgrass selection in southern climates favored a change from cool- to warm-season species, depending on the playing surface. Whether in historically cool-season or warm-season regions, it appears that many golf facilities are exploring alternatives to their traditional turfgrass species.

While suspended particles play many important roles in the marine environment, their concentrations are very small in the deep sea, making observation difficult with existing methods: water sampling, optical sensors, and special imaging systems. Methods are needed to fill the lack of environmental baseline data in the deep sea, ones that are inexpensive, quick, and intuitive. In this study we applied object detection using deep learning to evaluate the variability of suspended particle abundance from images taken by a common stationary camera, “Edokko Mark 1”. Images were taken in a deep-sea seamount in the Northwest Pacific Ocean for approximately one month. Using the particles in images as training data, an object detection algorithm YOLOv5 was used to construct a suspended particle detection model. The resulting model successfully detected particles in the image with high accuracy (AP50 > 85% and F1 Score > 82%). Similarly high accuracy for a site not used for model training suggests that model detection accuracy was not dependent on one specific shooting condition. During the observation period, the world’s first cobalt-rich ferromanganese crusts excavation test was conducted, providing an ideal situation to test this model’s ability to measure changes in suspended particle concentrations in the deep sea. The time series showed relatively little variability in particle counts under natural conditions, but there were two turbidity events during/after the excavation, and there was a significant difference in numbers of suspended particles before and after the excavation. These results indicate that this method can be used to examine temporal variations both in small amounts of naturally occurring suspended particles and large abrupt changes such as mining impacts. A notable advantage of this method is that it allows for the possible use of existing imaging data and may be a new option for understanding temporal changes of the deep-sea environment without requiring the time and expense of acquiring new data from the deep sea.

Abstract Herbicide carryover injury to rotational crops can vary in severity depending on the influence of soil properties on herbicide bioavailability. Greenhouse bioassays were conducted with soybean, radish, and canola to evaluate differences in the bioavailability of three herbicides with carryover risk, atrazine, mesosulfuron‐methyl, and topramezone. Bioassays were conducted in three varying regional soil types with nine herbicide treatment rates including a control. Plant visual injury was evaluated weekly, and aboveground dry biomass was weighed after harvest of soybean 28 days after emergence (DAE) and radish and canola 21 DAE. A log‐logistic dose–response regression model was used to quantify herbicide‐effective concentrations for 30% (EC30), 50% (EC50), and 80% (EC80) visual injury and aboveground dry biomass reduction in each soil type. Relative herbicide‐soil bioavailability was determined through comparisons of herbicide‐effective concentrations among soil types. Pearson correlation revealed that atrazine, mesosulfuron‐methyl, and topramezone EC30 for all species were positively correlated to soil organic matter (OM) content (r = 0.56, 0.48, and 0.40, respectively) and cation exchange capacity (CEC) (r = 0.43, 0.41, and 0.45). Topramezone EC80 for soybean and radish was positively correlated to soil clay content (r = 0.51) and silt content (r = 0.51) and negatively correlated to sand content (r = −0.51) and pH (r = −0.52). Decreased atrazine, mesosulfuron‐methyl, and topramezone bioavailability in soil with high OM and CEC, decreased topramezone bioavailability in coarse‐textured soil and at high soil pH, and differential herbicide sensitivity of crop species can inform grower decisions on herbicide selections and rotational crop plans.

Introduction and objective The Interdisciplinary Cardiac Arrest Research Review (ICARE) group was formed in 2018 to conduct an annual search of peer-reviewed literature relevant to cardiac arrest. Now in its third year, the goals of the review are to illustrate best practices in research and help reduce compartmentalization of knowledge by disseminating relevant advances in the interdisciplinary world of cardiac arrest research. This iteration focuses on pharmacology and basic and translational science contributions. Review methods A search was conducted of PubMed using keywords related to cardiac arrest. Titles and abstracts were screened for relevance with a focus on basic science and pharmacology. Screened manuscripts underwent standardized scoring of methodological quality and impact on the respective fields by reviewer teams lead by a subject matter expert editor. Articles scoring higher than 99 percentiles by category were selected for full critique. Systematic differences between editors’ and reviewers’ scores were assessed using Wilcoxon signed-rank test. Brief description of the state of knowledge The top scoring studies centered around attempts at improving neurologic outcome through improved blood flow and reduction of metabolic demand in order to reduce the impact of hypoxia during resuscitation on the brain. Summary The sheer number of articles screened is a testament to the need for an accessible source highlighting highquality and important research. Several high-quality systematic reviews and original research studies have provided a physiologic basis for the treatment of cardiac arrest, and make the case for focused progression of several pharmacologic treatments to larger animal and human trials.

Background: Eponyms are ubiquitous in dermatology; however, their usage trends have not been studied. Objective: To characterize the usage of eponyms in dermatology from 1880 to 2020. Methods: Candidate eponyms were collected from a textbook and an online resource. A subset of these eponyms was deemed to be dermatology-focused by a panel of experienced dermatologists. Python scripts were used to permute eponyms into multiple variations and automatically search PubMed using BioPython’s Entrez library. Results: The dermatologist panel designated 373 of 529 candidate eponyms as dermatology-focused. These eponyms were permuted into 3159 variations and searched in PubMed. The highest occurring dermatology-focused eponyms (DFEs) in the year 2020 included Leishmania, Behçet syndrome, Kaposi sarcoma, Langerhans cell histiocytosis, and Mohs surgery. Increased DFE usage in the general medical literature parallels the overall increase in the use of other eponyms in the medical literature. However, in the most cited dermatology journals, DFE usage did not increase in the past decade. There were several eponyms with decreased usage. Limitations: This study is limited to the publications in PubMed; only titles and abstracts could be queried. Conclusion: DFEs are increasing in usage in the general medical literature, but the usage of eponyms in the most cited dermatology journals has plateaued.

Technological advancement of vehicle platforms exposes opportunities for new attack paths and vulnerabilities. Static cyber defenses can help mitigate certain attacks, but those attacks must generally be known ahead of time, and the cyber defenses must be hand-crafted by experts. This research explores reinforcement learning (RL) as a path to achieve autonomous, intelligent cyber defense of vehicle control networks—namely, the controller area network (CAN) bus. We train an RL agent for the CAN bus using Toyota’s Portable Automotive Security Testbed with Adaptability (PASTA). We then apply the U.S. Army Combat Capabilities Development Command (DEVCOM) Army Research Laboratory’s methodology for quantitative measurement of cyber resilience to assess the agent’s effect on the vehicle testbed in a contested cyberspace environment. Despite all defenses having similar traditional performance measures, our RL agent averaged a 90% cyber resilience measurement during drive cycles executed on hardware versus 41% for a naïve static timing defense and 98% for the bespoke timing-based defense. Our results also show that an RL-based agent can detect and block injection attacks on a vehicle CAN bus in a laboratory environment with greater cyber resilience than prior learning approaches (1% for convolutional networks and 0% for recurrent networks). With further research, we believe there is potential for using RL in the autonomous intelligent cyber defense agent concept.

The Congo Basin is home to the second largest stretch of continuous tropical forest, but the magnitude of greenhouse fluxes are poorly understood. Here the authors analyze gas samples and find the region is not actually a hotspot of N2O emissions.

While the COVID-19 pandemic has had a detrimental impact on many businesses worldwide, essential businesses, such as grocery stores, continued to operate despite potential disease transmission. Although the principal mode by which people are infected with SARS-CoV-2, the virus that causes COVID-19, is through exposure to respiratory droplets and very small particles carrying infectious virus, contaminated surfaces might play a role in transmission. We collected swab samples from frequently touched surfaces, including grocery carts, touchscreen monitors, credit card keypads, pharmacy counters, self-service food utensils, and refrigerator and freezer handles, in two metro-Atlanta grocery stores over the course of two sampling events in March 2021. Of the 260 swab samples collected, 6 (2.3%) samples were positive for SARS-CoV-2 RNA by reverse transcriptase quantitative polymerase chain reaction. Positive samples were collected from pharmacy (12.0% [3/25] samples), refrigerator/freezer aisles (2.5% [1/39] samples), and self-service food court (5.0% [2/40] samples) areas. Table/counter edge and underside surfaces represented 33% (2/6) of positive samples. These data suggest that risk of exposure to SARS-CoV-2 from frequently touched surfaces in grocery store settings is likely low; however, more frequent cleaning of surfaces in pharmacy and self-service food courts might be warranted.

Tis-Abay Waterfall is a famous tourist destination in northern Ethiopia, attracting both domestic and international visitors. Although the site's recreation and tourism potential are enormous, the value of the waterfall remains underestimated; an application of economic valuation methods can provide information to better utilize the resource. This study estimates the recreational value of Tis-Abay Waterfall and analyzes the consumer characteristics associated with recreational demand. Drawing on data from 1044 on-site surveys, the study uses the Individual Travel Cost Method (ITCM) with a Zero-Truncated Poisson (ZTP) regression model. Results of the ZTP regression suggest that visitors' age, monthly income, and interest in alternative recreation sites like Lake Tana and Gondar Fasiledes Royal Castle are variables significantly and positively related with recreational demand for the Tis-Abay Waterfall. However, visitors' recreational demand is negatively associated with respondents' distance from the site, leisure time, and total cost of site access. The appraisal suggests that the Tis-Abay Waterfall has a significant annual recreational value of $9.5 million. But it also shows that the waterfall's value would increase significantly, up to $17.3 million, with hypothetical quality improvements in the waterfall settings. The value attachment suggests that estimating the recreation value for Tis-Abay Waterfall is a central component in the sustainable use and management of the resource. However, the presence of unfavorable trade-offs with the electric power plant and the inadequate infrastructure and services for reaching the site are major concerns that require immediate attention to make better use of Tis-Abay Waterfall's recreational services.

BackgroundPost-mortem microbial communities are increasingly investigated as proxy evidence for a variety of factors of interest in forensic science. The reported predictive power of the microbial community to determine aspects of the individual’s post-mortem history (e.g., the post-mortem interval) varies substantially among published research. This observed variation is partially driven by the local environment or the individual themselves. In the current study, we investigated the impact of BMI, sex, insect activity, season, repeat sampling, decomposition time, and temperature on the microbial community sampled from donated human remains in San Marcos, TX using a high-throughput gene-fragment metabarcoding approach.Materials and methodsIn the current study, we investigated the impact of BMI, sex, insect activity, season, repeat sampling, decomposition time, and temperature on the microbial community sampled from donated human remains in San Marcos, TX using a high-throughput gene-fragment metabarcoding approach.ResultsWe found that season, temperature at the sampling site, BMI, and sex had a significant effect on the post-mortem microbiome, the presence of insects has a homogenizing influence on the total bacterial community, and that community consistency from repeat sampling decreases as the decomposition process progresses. Moreover, we demonstrate the importance of temperature at the site of sampling on the abundance of important diagnostic taxa.ConclusionThe results of this study suggest that while the bacterial community or specific bacterial species may prove to be useful for forensic applications, a clearer understanding of the mechanisms underpinning microbial decomposition will greatly increase the utility of microbial evidence in forensic casework.

Purpose Caring for critically ill patients requires non-technical skills such as teamwork, communication, and task management. The Behaviorally Anchored Rating Scale (BARS) is a brief tool used to assess non-technical skills. The investigators determined inter- and intra-rater reliability of the BARS when used to assess medical students in simulated scenarios.Method The investigators created simulation scenarios for medical students during their pediatric clerkship. Content experts reviewed video recordings of the simulations and assigned BARS scores for four performance components (Situational Awareness, Decision-Making, Communication, and Teamwork) for the leader and for the team as a whole. Krippendorff’s alpha with ordinal difference was calculated to measure inter- and intra-rater reliability.Results Thirty medical students had recordings available for review. Inter- and intra-rater reliability for performance components were, respectively, Individual Situational Awareness (0.488, 0.638), Individual Decision-Making (0.529, 0.691), Individual Communication (0.347, 0.473), Individual Teamwork (0.414, 0.466), Team Situational Awareness (0.450, 0.593), Team Decision Making (0.423, 0.703), Team Communication (0.256, 0.517), and Team Teamwork (0.415, 0.490).Conclusions The BARS demonstrated limited reliability when assessing medical students during their pediatric clerkship. Given the unique needs of this population, a modified or new objective scoring system for assessing non-technical skills may be needed for medical students.

Abstract Drylands represent more than 41% of the global land surface and are at degradation risk due to land use and climate change. Developing strategies to mitigate degradation and restore drylands in the face of these threats requires an understanding of how drylands are shaped by not only soils and climate, but also geology and geomorphology. However, few studies have completed such a comprehensive analysis that relates spatial variation in plant communities to all aspects of the geologic–geomorphic–edaphic–plant–climate system. The focus of this study is the Colorado Plateau, a high‐elevation dryland in the southwestern United States, which is particularly sensitive to future change due to climate vulnerability and increasing land‐use pressure. Here, we examined 135 long‐term vegetation‐monitoring sites in three national parks and characterized connections between geology, geomorphology, soils, climate, and dryland plant communities. To first understand the geologic and geomorphic influences on soil formation and characteristics, we explore associations between soil pedons, bedrock geology, and geomorphology. Then, we characterize principal axes of variation in plant communities and ascertain controls and linkages between components of the edaphic–geomorphic system and plant community ordinations. Geologic and geomorphic substrate exerted controls on important properties of the soil profile, particularly depth, water‐holding capacity, rockiness, salinity, and fine sands. Ordination identified five distinct plant communities and three primary axes of variation, representing gradients of woody‐ to herbaceous‐dominated communities (Axis 1), saline scrublands to C3 grasslands (Axis 2), and annual to perennial communities (Axis 3). Geology, geomorphology, and soil explained a large proportion of variation in Axis 1 (74%), while climate variables largely explained Axis 2 (68%), and Axis 3 was not well explained by the random forest models. The variables identified as most influential to each axis were, respectively: (1) soil depth; (2) aridity, lithology, and soil salinity; and (3) temperature and precipitation. We posit that Axis 3 represents a land degradation gradient due to historic grazing, likely exacerbated by dry conditions. Results provide a novel framework that links the geologic and geomorphic evolution of landscapes, with the distribution of soils and plant communities that can guide ecosystem management, exemplifying an approach applicable to drylands globally.

Nowhere is biomass burning more abundant than on the African continent, but the biogeochemical impacts on forests are poorly understood. Here the authors show that biomass burning leads to high phosphorus deposition in the Congo basin, which scales with forest age as a result of increasing canopy complexity.

Abstract Objectives The Interdisciplinary Cardiac Arrest Research Review (ICARE) group was formed in 2018 to conduct an annual search of peer‐reviewed literature relevant to cardiac arrest. Now in its third year, the goals of the review are to highlight annual updates in the interdisciplinary world of clinical cardiac arrest research with a focus on clinically relevant and impactful clinical and population‐level studies from 2020. Methods A search of PubMed using keywords related to clinical research in cardiac arrest was conducted. Titles and abstracts were screened for relevance and sorted into 7 categories: Epidemiology & Public Health Initiatives; Prehospital Resuscitation, Technology & Care; In‐Hospital Resuscitation & Post‐Arrest Care; Prognostication & Outcomes; Pediatrics; Interdisciplinary Guidelines & Reviews; and a new section dedicated to the coronavirus disease 2019 (COVID‐19) pandemic. Screened manuscripts underwent standardized scoring of methodological quality and impact on the respective fields by reviewer teams lead by a subject matter expert editor. Articles scoring higher than 99 percentiles by category were selected for full critique. Systematic differences between editors’ and reviewers’ scores were assessed using Wilcoxon signed‐rank test. Results A total of 3594 articles were identified on initial search; of these, 1026 were scored after screening for relevance and deduplication, and 51 underwent full critique. The leading category was Prehospital Resuscitation, Technology & Care representing 35% (18/51) of fully reviewed articles. Four COVID‐19 related articles were included for formal review that was attributed to a relative lack of high‐quality data concerning cardiac arrest and COVID‐19 specifically by the end of the 2020 calendar year. No significant differences between editor and reviewer scoring were found among review articles (P = 0.697). Among original research articles, section editors scored a median 1 point (interquartile range, 0–3; P

Opioid analgesics such as morphine and fentanyl induce mu-opioid receptor (MOR)-mediated hyperactivity in mice. Herein, we show that morphine, fentanyl, SR-17018, and oliceridine have submaximal intrinsic efficacy in the mouse striatum using 35S-GTPγS binding assays. While all of the agonists act as partial agonists for stimulating G protein coupling in striatum, morphine, fentanyl, and oliceridine are fully efficacious in stimulating locomotor activity; meanwhile, the noncompetitive biased agonists SR-17018 and SR-15099 produce submaximal hyperactivity. Moreover, the combination of SR-17018 and morphine attenuates hyperactivity while antinociceptive efficacy is increased. The combination of oliceridine with morphine increases hyperactivity, which is maintained over time. These findings provide evidence that noncompetitive agonists at MOR can be used to suppress morphine-induced hyperactivity while enhancing antinociceptive efficacy; moreover, they demonstrate that intrinsic efficacy measured at the receptor level is not directly proportional to drug efficacy in the locomotor activity assay.

Diet-induced models of chronic kidney disease (CKD) offer several advantages, including clinical relevance and animal welfare, compared with surgical models. Oxalate is a plant-based, terminal toxic metabolite that is eliminated by the kidneys through glomerular filtration and tubular secretion. An increased load of dietary oxalate leads to supersaturation, calcium oxalate crystal formation, renal tubular obstruction, and eventually CKD. Dahl-Salt-Sensitive (SS) rats are a common strain used to study hypertensive renal disease; however, the characterization of other diet-induced models on this background would allow for comparative studies of CKD within the same strain. In the present study, we hypothesized that SS rats on a low-salt, oxalate rich diet would have increased renal injury and serve as novel, clinically relevant and reproducible CKD rat models. Ten-week-old male SS rats were fed either 0.2% salt normal chow (SS-NC) or a 0.2% salt diet containing 0.67% sodium oxalate (SS-OX) for five weeks.Real-time PCR demonstrated an increased expression of inflammatory marker interleukin-6 (IL-6) (p < 0.0001) and fibrotic marker Timp-1 metalloproteinase (p < 0.0001) in the renal cortex of SS-OX rat kidneys compared with SS-NC. The immunohistochemistry of kidney tissue demonstrated an increase in CD-68 levels, a marker of macrophage infiltration in SS-OX rats (p < 0.001). In addition, SS-OX rats displayed increased 24 h urinary protein excretion (UPE) (p < 0.01) as well as significant elevations in plasma Cystatin C (p < 0.01). Furthermore, the oxalate diet induced hypertension (p < 0.05). A renin–angiotensin–aldosterone system (RAAS) profiling (via liquid chromatography–mass spectrometry; LC–MS) in the SS-OX plasma showed significant (p < 0.05) increases in multiple RAAS metabolites including angiotensin (1–5), angiotensin (1–7), and aldosterone. The oxalate diet induces significant renal inflammation, fibrosis, and renal dysfunction as well as RAAS activation and hypertension in SS rats compared with a normal chow diet. This study introduces a novel diet-induced model to study hypertension and CKD that is more clinically translatable and reproducible than the currently available models.

Field studies were conducted in 2016, 2017, and 2020 in the south-central and Coastal Bend regions of Texas to determine the effects of various biostimulants, soil additives, and plant protectants on corn growth and yield. In south-central Texas, the use of pop-up fertilizer (9-30-0 + Zn) either alone or in combination with either 2% N, bifenthrin, or bifenthrin + pyraclostrobin resulted in the greatest corn vigor but a yield response was only noted with pop-up fertilizer alone at 28,062 or 46,771 mL ha−1 in one year. In the Coastal Bend region, leaf tissue analysis showed that only Fe was affected with the use of any soil additive. Bacillus licheniformis + Bacillus megaterium + Bacillus pumilus increased Fe leaf tissue content by 20% over the untreated check. Radicoat seed coating at 438 mL ha−1 reduced corn plant stand by 10%, and Pseudomonas brassicaceanum reduced corn height when compared with the untreated check; however, no differences in test weight or yield from the untreated check were noted with any soil additives. Little if any impacts of the use of biostimulants, soil amendments, or plant protectants were seen in these studies.

Background: Occupational physicians work directly with individual employees regarding diseases that has been caused or exacerbated by workplace factors. However, employees are increasingly required to travel for their work, including to tropical countries where they risk exposure to diseases they would not normally encounter at home (i.e., malaria). Such disease/s may also take days to months to incubate before becoming symptomatic, even after their return home, thus delaying and complicating the diagnosis. Proving this was an occupational disease with respective sick leave entitlement or compensation can be challenging. There is a lack of data concerning occupational diseases caused by tropical infections. Material and methods: Employee case records for the period 2003-2008 from the State Institute for Occupational Health and Safety of North-Rhine Westphalia in Germany were analysed and assessed within Germany’s regulatory framework. These records included Germany’s largest industrial zone. Results: From 2003-2008the suspected cases of “tropical diseases and typhus”, categorized as occupational disease “Bk 3104” in Germany, have decreased significantly. A high percentage of the suspected cases was accepted as occupational disease, but persistent or permanent sequelae which conferred an entitlement to compensation were rare. Conclusion: There is scope to improve diagnosis and acceptance of tropical diseases as occupational diseases. The most important diseases reported were malaria, amoebiasis, and dengue fever. Comprehensive pre-travel advice and post-travel follow-ups by physicians trained in travel and occupational health medicine should be mandatory. Data indicate that there is a lack of knowledge on how to prevent infectious disease abroad.

We conducted a long-term inventory of the herpetofauna of Wildsumaco Wildlife Sanctuary in the eastern part of the Napo Province in Ecuador. This private preserve is about 500 ha in size and is located on the southern slopes of Volcán Sumaco. The preserve contains primary forest, secondary forest, and pasture habitats. Based mostly on nocturnal transect sampling we documented 39 species of amphibians including one species of salamander, two species of caecilians, and 36 species of frogs. Rain frogs (Pristimantis Jiminez de la Espada, 1871) were diverse with 14 species documented. A diverse species assemblage of 45 reptile species was also documented on the preserve. Six amphibian species found were listed by the IUCN Red List. At least three species exhibited substantial geographic range extensions and seven species showed elevational range extensions. We discovered several undescribed species including one salamander, three frogs, one lizard, and one snake.

Objective: To provide a step-by-step presentation of the complete primary repair of bladder exstrophy (CPRE). Design: Video presentation. Setting: Children's Hospital of Philadelphia, within the Multi-Institutional Bladder Exstrophy Collaboration Patient(s): A 3-month-old boy with the bladder exstrophy (BE) undergoing a CPRE after the family provided informed consent. Results: A ten-hour procedure, including a bilateral pelvic osteotomy and epidural catheter placement was completed. There was no intraoperative complication. The child was immobilized with a spica cast for 6 weeks. His bladder was cycled via the suprapubic tube until he could tolerate clamping for 24 h with no evidence of upper tract dilation. Conclusions: Through collaboration, we have significantly refined the CPRE technique for BE repair. We believe that for rare congenital anomalies such as bladder exstrophy, collaboration across institutions increases volume exposure, which in turn, correlates with improved outcomes. We continue to carefully follow treated patients for the long-term to observe these outcomes, and measure the burden of BE on families in terms of stigma, quality of life and psychosocial impacts.

Phenological models representing physiological and behavioral processes of organisms are used to study, predict, and optimize management of ecological subsystems. One application of phenological models is the prediction of temporal intervals associated with the measurable physiological development of arthropods, for the purpose of estimating future time points of interest such as the emergence of adults, or estimating past time points such as the arrival of ovipositing females to new resources. The second of these applications is of particular use in the conduct of forensic investigations, where the time of a suspicious death must be estimated on the basis of evidence, including arthropods with measurable size/age, found at the death scene. Because of the longstanding practice of using necrophagous insects to estimate time of death, standardized data and methods exist. We noticed a pattern in forensic entomological validation studies: bias in the values of a model parameter is associated with improved model fit to data, for a reason that is inconsistent with how the models used in this practice are interpreted. We hypothesized that biased estimates for a threshold parameter, representing the lowest temperature at which insect development is expected to occur, result in models’ accounting for behavioral and physiological thermoregulation but in a way that results in low predictive reliability and narrowed applicability of models involving these biased parameter estimates. We explored a more realistic way to incorporate thermoregulation into insect phenology models with forensic entomology as use context, and found that doing so results in improved and more robust predictive models of insect phenology.

Heat stress (HS) is devastating to poultry production sustainability due its detrimental effects on performance, welfare, meat quality, and profitability. One of the most known negative effects of HS is feed intake depression, which is more pronounced in modern high-performing broilers compared to their ancestor unselected birds, yet the underlying molecular mechanisms are not fully defined. The present study aimed, therefore, to determine the hypothalamic expression of a newly involved pathway, hypoxia/oxygen homeostasis, in heat-stressed broiler-based research lines and jungle fowl. Three populations of broilers (slow growing ACRB developed in 1956, moderate growing 95RB from broilers available in 1995, and modern fast growing MRB from 2015) and unselected Jungle fowl birds were exposed to cyclic heat stress (36°C, 9 h/day for 4 weeks) in a 2 × 4 factorial experimental design. Total RNAs and proteins were extracted from the hypothalamic tissues and the expression of target genes and proteins was determined by real-time quantitative PCR and Western blot, respectively. It has been previously shown that HS increased core body temperature and decreased feed intake in 95RB and MRB, but not in ACRB or JF. HS exposure did not affect the hypothalamic expression of HIF complex, however there was a line effect for HIF-1α (P = 0.02) with higher expression in JF under heat stress. HS significantly up regulated the hypothalamic expression of hemoglobin subunits (HBA1, HBBR, HBE, HBZ), and HJV in ACRB, HBA1 and HJV in 95RB and MRB, and HJV in JF, but it down regulated FPN1 in JF. Additionally, HS altered the hypothalamic expression of oxygen homeostasis- up and down-stream signaling cascades. Phospho-AMPKThr172 was activated by HS in JF hypothalamus, but it decreased in that of the broiler-based research lines. Under thermoneutral conditions, p-AMPKThr172 was higher in broiler-based research lines compared to JF. Ribosomal protein S6K1, however, was significantly upregulated in 95RB and MRB under both environmental conditions. HS significantly upregulated the hypothalamic expression of NF-κB2 in MRB, RelB, and TNFα in ACRB, abut it down regulated RelA in 95RB. The regulation of HSPs by HS seems to be family- and line-dependent. HS upregulated the hypothalamic expression of HSP60 in ACRB and 95RB, down regulated HSP90 in JF only, and decreased HSP70 in all studied lines. Taken together, this is the first report showing that HS modulated the hypothalamic expression of hypoxia- and oxygen homeostasis-associated genes as well as their up- and down-stream mediators in chickens, and suggests that hypoxia, thermotolerance, and feed intake are interconnected, which merit further in-depth investigations.