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14 results on '"Trappe, Susanne"'

3. Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine

Author

Trachsel, Dagmar S., primary, Stage, Hannah J., additional, Rausch, Sebastian, additional, Trappe, Susanne, additional, Söllig, Katharina, additional, Sponder, Gerhard, additional, Merle, Roswitha, additional, Aschenbach, Jörg R., additional, and Gehlen, Heidrun, additional

Published
2022
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4. Low Magnesium Concentration Enforces Bone Calcium Deposition Irrespective of 1,25-Dihydroxyvitamin D 3 Concentration.

Author

Rashid, Usman, Becker, Sandra K., Sponder, Gerhard, Trappe, Susanne, Sandhu, Mansur A., and Aschenbach, Jörg R.

Subjects
MESENCHYMAL stem cells, GENE expression, MAGNESIUM, CALCIUM, ALKALINE phosphatase, STEM cells
Abstract

Efficient coordination between Mg2+ and vitamin D maintains adequate Ca2+ levels during lactation. This study explored the possible interaction between Mg2+ (0.3, 0.8, and 3 mM) and 1,25-dihydroxyvitamin D3 (1,25D; 0.05 and 5 nM) during osteogenesis using bovine mesenchymal stem cells. After 21 days, differentiated osteocytes were subjected to OsteoImage analysis, alkaline phosphatase (ALP) activity measurements, and immunocytochemistry of NT5E, ENG (endoglin), SP7 (osterix), SPP1 (osteopontin), and the BGLAP gene product osteocalcin. The mRNA expression of NT5E, THY1, ENG, SP7, BGLAP, CYP24A1, VDR, SLC41A1, SLC41A2, SLC41A3, TRPM6, TRPM7, and NIPA1 was also assessed. Reducing the Mg2+ concentration in the medium increased the accumulation of mineral hydroxyapatite and ALP activity. There was no change in the immunocytochemical localization of stem cell markers. Expression of CYP24A1 was higher in all groups receiving 5 nM 1,25D. There were tendencies for higher mRNA abundance of THY1, BGLAP, and NIPA1 in cells receiving 0.3 mM Mg2+ and 5 nM 1,25D. In conclusion, low levels of Mg2+ greatly enhanced the deposition of bone hydroxyapatite matrix. The effect of Mg2+ was not modulated by 1,25D, although the expression of certain genes (including BGLAP) tended to be increased by the combination of low Mg2+ and high 1,25D concentrations. [ABSTRACT FROM AUTHOR]

Published
2023
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5. The Combined Influence of Magnesium and Insulin on Central Metabolic Functions and Expression of Genes Involved in Magnesium Homeostasis of Cultured Bovine Adipocytes

Author

Becker, Sandra K., Sponder, Gerhard, Sandhu, Mansur A., Trappe, Susanne, Kolisek, Martin, and Aschenbach, J��rg R.

Subjects
insulin, ketosis, QH301-705.5, glycerol-3-phosphate dehydrogenase, adipocytes, magnesium, Lipid Metabolism, Article, adipose tissue, Chemistry, Glucose, Gene Expression Regulation, NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases, cattle, magnesium-responsive genes, Animals, Homeostasis, Biology (General), Energy Metabolism, lipomobilization, QD1-999, Cells, Cultured, 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::636 Viehwirtschaft, fatty liver
Abstract

At the onset of lactation, dairy cows suffer from insulin resistance, insulin deficiency or both, similar to human diabetes, resulting in lipolysis, ketosis and fatty liver. This work explored the combined effects of different levels of magnesium (0.1, 0.3, 1 and 3 mM) and insulin (25, 250 and 25,000 pM) on metabolic pathways and the expression of magnesium-responsive genes in a bovine adipocyte model. Magnesium starvation (0.1 mM) and low insulin (25 pM) independently decreased or tended to decrease the accumulation of non-polar lipids and uptake of the glucose analog 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-6-deoxyglucose (6-NBDG). Activity of glycerol 3-phosphate dehydrogenase (GPDH) was highest at 25 pM insulin and 3 mM magnesium. Expression of SLC41A1 and SLC41A3 was reduced at 0.1 mM magnesium either across insulin concentrations (SLC41A1) or at 250 pM insulin (SLC41A3). MAGT1 expression was reduced at 3 mM magnesium. NIPA1 expression was reduced at 3 mM and 0.1 mM magnesium at 25 and 250 pM insulin, respectively. Expression of SLC41A2, CNNM2, TRPM6 and TRPM7 was not affected. We conclude that magnesium promotes lipogenesis in adipocytes and inversely regulates the transcription of genes that increase vs. decrease cytosolic magnesium concentration. The induction of GAPDH activity by surplus magnesium at low insulin concentration can counteract excessive lipomobilization.

Published
2021

12. Production of tissue plasminogen activator (t-PA) inAspergillus niger

Author

Wiebe, Marilyn G., primary, Karandikar, Atul, additional, Robson, Geoff D., additional, Trinci, Anthony P. J., additional, Candia, Juana-L Flores, additional, Trappe, Susanne, additional, Wallis, Gregg, additional, Rinas, Ursula, additional, Derkx, Patrick M. F., additional, Madrid, Susan M., additional, Sisniega, Heidi, additional, Faus, Ignacio, additional, Montijn, Roy, additional, van den Hondel, Cees A. M. J. J., additional, and Punt, Peter J., additional

Published
2001
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14. Production of tissue plasminogen activator (t-PA) in <TOGGLE>Aspergillus niger</TOGGLE>

Author

Wiebe, Marilyn G., Karandikar, Atul, Robson, Geoff D., Trinci, Anthony P. J., Candia, Juana-L Flores, Trappe, Susanne, Wallis, Gregg, Rinas, Ursula, Derkx, Patrick M. F., Madrid, Susan M., Sisniega, Heidi, Faus, Ignacio, Montijn, Roy, Hondel, Cees A. M. J. J. van den, and Punt, Peter J.

Abstract

A protease-deficient strain of Aspergillus niger has been used as a host for the production of human tissue plasminogen activator (t-PA). In defined medium, up to 0.07 mg t-PA (g biomass)−1 was produced in batch and fed-batch cultures and production was increased two- to threefold in two-phase batch cultures in which additional glucose was provided as a single pulse at the end of the first batch growth phase. Production was increased [up to 1.9 mg t-PA (g biomass)−1] by the addition of soy peptone to the defined medium. The rate of t-PA production in batch cultures supplemented with soy peptone (0.2 to 0.6 mg t-PA L−1 h−1) was comparable to rates observed previously in high-producing mammalian or insect cell cultures. In glucose-limited chemostat culture supplemented with soy peptone, t-PA was produced at a rate of 0.7 mg t-PA L−1 h−1. Expression of t-PA in A. niger resulted in increased expression of genes (bipA, pdiA, and cypB) involved in the unfolded protein response (UPR). However, when cypB was overexpressed in a t-PA-producing strain, t-PA production was not increased. The t-PA produced in A. niger was cleaved into two chains of similar molecular weight to two-chain human melanoma t-PA. The two chains appeared to be stable for at least 16 h in culture supernatant of the host strain. However, in general, &lt;1% of the t-PA produced in A. niger was active, and active t-PA disappeared from the culture supernatant during the stationary phase of batch cultures, suggesting that the two-chain t-PA may have been incorrectly processed or that initial proteolytic cleavage occurred within the proteolytic domain of the protein. Total t-PA (detected by enzyme-linked immunoassay) also eventually disappeared from culture supernatants, confirming significant extracellular proteolytic activity, even though the host strain was protease-deficient. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 76: 164–174, 2001.

Published
2001
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