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The use of salt-tolerant plant growth promoting rhizobacteria (ST-PGPR) for reducing environmental stress and enhancing quality of crops has recently emerged as a perspective for sustainable development of agriculture, especially in the context of climate change. Within the mining of potential bacterial candidates serving for such purposes, bacterial strains from rhizosphere of mangrove plants in Vietnam were isolated and characterized. Results revealed two microbial isolates from Sonneratia caseolaris, symbolized as S13 and S15, exhibited 1-aminocyclopropane-l-carboxylic acid (ACC) deaminase activity and salt tolerance up to 7% sodium chloride. These bacteria performed phosphate solubilizing property with 57.04-61.16% of degradation after 9 days in NBRIP medium. Besides, S13 and S15 showed antimicrobial activity against plant pathogens, including F. oxysporum and A. niger. By analyzing the ribosomal 16S sequences, bacterial strains S13 and S15 were identified to be Bacillus spp. with phylogenetically close relation to B. velezensis and B. siamensis, respectively. Additionally, sprouts encoated with S13 and S15 bacterial suspensions (~4*108 CFU/L each) exhibited enhanced shoot and root elongation in both normal and saline conditions. The bacterial strains were in vitro evaluated to be nontoxic to tested cells and animals. Achieved results brought out mangrove derived ST-PGPR strains with highly applicable potential for development of saline agriculture. [ABSTRACT FROM AUTHOR]

[This corrects the article DOI: 10.1371/journal.ppat.1010268.].

Next generation sequencing has revealed the presence of numerous RNA viruses in animal reservoir hosts, including many closely related to known human pathogens. Despite their zoonotic potential, most of these viruses remain understudied due to not yet being cultured. While reverse genetic systems can facilitate virus rescue, this is often hindered by missing viral genome ends. A prime example is Lloviu virus (LLOV), an uncultured filovirus that is closely related to the highly pathogenic Ebola virus. Using minigenome systems, we complemented the missing LLOV genomic ends and identified cis-acting elements required for LLOV replication that were lacking in the published sequence. We leveraged these data to generate recombinant full-length LLOV clones and rescue infectious virus. Similar to other filoviruses, recombinant LLOV (rLLOV) forms filamentous virions and induces the formation of characteristic inclusions in the cytoplasm of the infected cells, as shown by electron microscopy. Known target cells of Ebola virus, including macrophages and hepatocytes, are permissive to rLLOV infection, suggesting that humans could be potential hosts. However, inflammatory responses in human macrophages, a hallmark of Ebola virus disease, are not induced by rLLOV. Additional tropism testing identified pneumocytes as capable of robust rLLOV and Ebola virus infection. We also used rLLOV to test antivirals targeting multiple facets of the replication cycle. Rescue of uncultured viruses of pathogenic concern represents a valuable tool in our arsenal for pandemic preparedness.

Aims To describe the drug use practices among female sex workers (FSWs) in Hanoi and to identify factors associated with their drug injecting. Design, setting and participants A two-stage cluster survey of 400 FSWs was conducted from June to September, 2002. Participating FSWs were both establishment- (160) and street-based (240), who were practising in seven urban and one suburban districts of Hanoi. Measurements Subjects were interviewed face to face using a structured questionnaire. Findings Among the middle-class FSWs, 27% used drugs, of whom 79% injected. Among low-class FSWs, 46% used drugs and 85% injected. Among drug-using FSWs, 86% had started using drugs within the past 6 years. Among drug-injecting FSWs, 81% had started injecting within the past 4 years. Cleaning of injecting equipment was not common among those who shared. Having drug-injecting 'love mates', drug-using clients, longer residence in Hanoi, more clients and not currently cohabiting were found to be independently associated with drug injecting among FSWs. Conclusions The high prevalence of injecting drug use among FSWs makes them susceptible to HIV infection, and is a threat to their clients. There is a strong relationship between drug-using FSWs and male drug-using clients and non-client partners. Intervention to prevent drug use initiation among non-drug-using FSWs and harm reduction among drug-using FSWs are urgently needed.

The strengthening ability associated with adding a gellan gum as an additive to bentonite slurry for use in coating soil surfaces was studied. Bentonite powder was mixed with hot gellan gum hydrogel (i.e., at 200 °C) to different gellan gum concentrations (i.e., 0, 3, 4, 5, 6, and 7% of the mass of distilled water). A series of unconfined compressive strength tests were conducted on gellan gum-bentonite mixtures subjected to various thermal curing conditions. The shrinkage behavior was observed during the thermal curing process. The research also studied the sand size particle effect on the strength and volume change of the dried gellan gum-modified bentonite. The test results showed the soil strengthening effect of adding gellan gum to bentonite slurry which was then added it to bentonite/silica sand mixtures. [ABSTRACT FROM AUTHOR]