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BACKGROUND: Multiple clinical observational studies have suggested a close relationship of serum trace elements and nutrients with osteonecrosis, but it remains unclear whether there is a genetic causal effect between serum trace elements and nutrients on osteonecrosis. OBJECTIVE: To investigate the causal effects of serum trace elements and nutrients on osteonecrosis using the Mendelian randomization approach. METHODS: The exposure factors of serum trace elements and vitamins with mononucleotide polymorphisms were obtained from the published UK Biobank database and publicly available databases of genome-wide association studies. The outcome event of osteonecrosis was derived from the FinnGen Biobank database. Mendelian randomization methods were employed to explore the causal relationship between seven trace elements and three nutrients with osteonecrosis. Causal inference was conducted using inverse variance weighting, MR-Egger, and weighted median methods. F-statistic was calculated to ensure the robustness of instrumental variables. Cochran's Q test and leave-one-out method were used for heterogeneity testing. MR-Egger regression and MR-PRESSO were employed for horizontal pleiotropy testing. PhenoScanner database was utilized to remove mononucleotide polymorphisms with horizontal pleiotropy to ensure the reliability of the results. RESULTS AND CONCLUSION: Causal relationships were found between serum selenium, phosphate, vitamin C, vitamin E, and osteonecrosis through Mendelian randomization analysis. Serum selenium, vitamin C, and vitamin E were found to have a protective effect on osteonecrosis, while excessive intake of phosphate increased the risk of osteonecrosis. No heterogeneity or horizontal pleiotropy was observed during the study, and Mendelian randomization statistical power (Power value > 80%) indicated the reliability of the aforementioned four results. These findings have important clinical implications for the development of targeted preventive and therapeutic measures for osteonecrosis. [ABSTRACT FROM AUTHOR]

Background: Gallstones are a common digestive disorder that threatens human health. Iron deficiency may be related to the formation of gallstones, but there is limited current epidemiological research. The objective of this study was to investigate the relationship between iron status and gallstones. Methods: The datasets from the National Health and Nutrition Examination Survey (NHANES) 2017–2020 were used in a cross-sectional investigation. Gallstones were determined by using the 2007–2010 NHANES questionnaire. Multivariate linear regression models were used to examine the association between serum iron, serum ferritin and iron intake with the risk for gallstones. Subgroup analysis based on gender, age, race, and diabetes were performed. Fitted smoothing curves were used to describe the linear relationship. Results: The research involved 7847 participants aged 20 and above, among whom 845 were identified as having gallstones. Participants with higher serum iron levels tended to have a lower gallstones prevalence. A negative relationship between serum iron and gallstones prevalence was observed (OR = 0.979, 95% CI:0.965–0.992). The group with the highest serum iron tertile had a 23.7% lower risk of gallstones compared to the lowest tertile (OR = 0.763, 95% CI:0.628‒0.929). Gallstone prevalence was inversely correlated with iron intake in model 1. The negative association between serum iron and gallstones remained stable in stratifications, including gender, age, race, and diabetes. Conclusions: Elevated serum iron was associated with a decreased prevalence of gallstones. However, to confirm the impact of long-term iron metabolism on gallstone formation, additional prospective research is necessary. [ABSTRACT FROM AUTHOR]

The minor and trace element compositions of biogenic carbonates such as foraminifera are important tools in paleoceanography research. However, most studies have focused primarily on samples with element to calcium (El/Ca) ratios higher than the El/Ca range often found in benthic foraminifera. Here, we systematically assess the precision and accuracy of foraminifera elemental analysis across a wide range of El/Ca especially at relatively low ratios, using a method on a Thermo Scientific iCAP Qc quadrupole Inductively Coupled Plasma Mass Spectrometer (ICP‐MS). We focus on two benthic foraminifera species, Hoeglundina elegans and Cibicidoides pachyderma, and prepared a suite of solution standards based on their typical El/Ca ranges to correct for signal drift and matrix effects during ICP‐MS analysis and to determine analytical precision. We observe comparable precisions with published studies at high El/Ca, and higher relative standard deviations for each element at lower El/Ca, as expected from counting statistics. The overall long‐term analytical precision (2σ) of the H. elegans‐like consistency standard solutions was 6.5%, 4.6%, 5.0%, for Li/Ca, Mg/Ca, Mg/Li, and 6.4%, 10.0%, 4.2% for B/Ca, Cd/Ca, Sr/Ca. The precision for H. elegans‐like Mg/Li is equivalent to a temperature uncertainty of 0.5–1.1°C. Measurement precisions were also assessed based on three international standards (one solution and two powder standards) and replicate measurements of H. elegans and C. pachyderma samples. We provide file templates and program scripts that can be used to design calibration and consistency standards, prepare run sequences, and convert the raw ICP‐MS data into molar ratios. Key Points: Higher relative standard deviations are reported at lower element/calcium values, as expected from counting statisticsConsistency standards having similar ratios to the unknown samples provide an accurate estimate of errorsFor the Hoeglundina elegans Mg/Li—temperature proxy, analytical precision (2σ) is equivalent to a temperature uncertainty of 0.5–1.1°C [ABSTRACT FROM AUTHOR]

Heavy metal levels have steadily risen over the past century due to the presence of human activity and are hazardous to human health and the environment. Regarding the health of humans and the environment, palladium (Pd) is among the most hazardous and toxic heavy metals. It is listed as a priority contaminant by the Agency for Toxic Substances and Disease Registry (ATSDR). As a result, eliminating pollution and tracking changes in Pd contamination in the air are the top research priorities. This study will be conducted on trees grown in Düzce, the fifth dirtiest city on the European continent, to identify appropriate species that may be utilized for tracking and mitigating Pd pollution in the air. Samples from Cupressus arizonica, Pinus pinaster, Cedrus atlantica, Pseudotsuga menziesii, and Picea orientalis will all be analyzed as part of the study, and the variations in Pd content according to species, organ, and direction will be assessed. The goal is to ascertain which biomonitors are best suited for tracking and mitigating lead contamination in the atmosphere. The study consistently found Pd contents in woods with different directions that developed within the same period. However, when the species were compared, notable differences were detected between the Pd levels formed in different directions in the same period for each species and the wood formed in the same direction in other periods. This variation suggests that Pd transfer in wood is restricted in all tested species. Consequently, every species performs as a reliable biomonitor for tracking Pd contamination. [ABSTRACT FROM AUTHOR]

Erosion and leaching of metal(loid)s from contaminated sites can spread pollution to adjacent ecosystems and be a source of toxicity for living organisms. Phytostabilization consists of selecting plant species accumulating little or no metal(loid)s in aerial parts to establish a vegetation cover and thus to stabilize the contaminants in the soil. Seeds of white lupin, common vetch, and buckwheat were sown in greenhouse on soils from former French mines (Pontgibaud and Vaulry) contaminated with metal(loid)s including high concentrations of As and Pb (772 to 1064 and 121 to 12,340 mg kg−1, respectively). After 3 weeks of exposure, the growth of white lupin was less affected than that of the 2 other species probably because metal(loid) concentrations in roots and aerial parts of lupins were lower (5–20 times less Pb in lupin leaves on Pontgibaud soil and 5–10 times less As in lupin leaves on Vaulry soil than in vetch and buckwheat). To limit oxidation and/or scavenge metal(loid)s, white lupin increased the content of proline and total phenolic compounds (TPC) in leaves and roots by a factor 2 whereas buckwheat stimulated the production of TPC by a factor 1.5–2, and non-protein thiols (NPT) by factors around 1.75 in leaves and 6–12 in roots. Vetch accumulated more proline than white lupin but less NPT than buckwheat and less TPC than the 2 other plant species. The level of oxidation was however higher than in control plants for the 3 species indicating that defense mechanisms were not completely effective. Overall, our results showed that white lupin was the best species for phytostabilization but amendments should be tested to improve its tolerance to metal(loid)s. [ABSTRACT FROM AUTHOR]

Trace element analysis of otoliths from core to edge was used to reveal differences in habitats of larvae and early juveniles of Japanese jack mackerel (Trachurus japonicus) caught in the East China Sea (ECS), Pacific Ocean, and Sea of Japan. Multi‐element signatures (Sr:Ca, Mg:Ca, Na:Ca, K:Ca, and Ba:Ca) were analyzed with multivariate statistics to determine whether these element signatures provide insight into regional population structure. The median values of elements: Ca ratios in otolith core region differed significantly among areas, with most elements between the ECS and the Pacific Ocean or Sea of Japan showing significant differences. The Sr:Ca ratios exhibited a V‐shaped trend from the otolith core to the edge, which was likely related to the vertical habitat layer shift of larvae and early juveniles of T. japonicus. Canonical analysis of principal coordinates using the element: Ca ratios and the hatching day were re‐classified according to the sampling area with higher accuracy in the ECS (88–95%) and the Sea of Japan (76–83%) compared to the Pacific Ocean (69–72%). These results indicate that the proportion of eggs, larvae, and early juveniles transported by the Kuroshio Current from the southern ECS—the main spawning ground—to the Pacific Ocean or the Sea of Japan is low and that juveniles distributed in the coastal waters off southwestern Japan may have originated from local spawners. These results suggest that determining the elemental composition of larvae and juveniles of T. japonicus is effective in determining the habitat differences of this species in the three regions. These findings will help to understand population structure and recruitment process of this species around Japan. [ABSTRACT FROM AUTHOR]

Trace elements released into the atmosphere by human activities, such as fossil fuel combustion, flow into forest ecosystems via wet and dry deposition and can flow out via mountainous stream water. The Kajikawa catchment site in Niigata Prefecture, located on the Sea of Japan side in central Japan, suffers from large amounts of acidic substance deposition due to its geographical conditions and meteorological seasonality and is affected by transboundary air pollution originating from the Asian continent due to northwesterly seasonal winds in winter. This study determined the concentrations of trace elements dissolved in precipitation and stream water to comprehensively evaluate the effects of transboundary air pollution on forest ecosystems. The results showed that the concentrations of trace elements and major inorganic ions in precipitation tended to be high in winter and low in summer. The fluxes of many trace elements also increased in winter, reflecting high precipitation amount. Except for Sr, the enrichment factors of the highly enriched trace elements did not show clear seasonality. Therefore, they were continuously influenced by anthropogenic activities. Furthermore, the stable Pb isotope ratio changed significantly during the observation period; however, this was not explained by seasonal changes. This study revealed that trace elements are transported to the Sea of Japan side in central Japan, especially in winter; however, their quantity and content change in response to changes in local and transboundary air pollution. [ABSTRACT FROM AUTHOR]

It is widely known that sex differences have a significant impact on patients with major depressive disorder (MDD). This study aims to evaluate the sex-related connection between serum trace elements and changes in neurometabolism in the anterior cingulate cortex (ACC) of MDD patients. 109 untreated MDD patients and 59 healthy controls underwent proton magnetic resonance spectroscopy (1H-MRS) under resting conditions. We measured metabolic ratios in the ACC from both sides. Additionally, venous blood samples were taken from all participants to detect calcium (Ca), phosphorus, magnesium (Mg), copper (Cu), ceruloplasmin (CER), zinc (Zn), and iron (Fe) levels. We performed association and interaction analyses to explore the connections between the disease and gender. In individuals with MDD, the Cu/Zn ratio increased, while the levels of Mg, CER, Zn and Fe decreased. Male MDD patients had lower Cu levels, while female patients had an increased Cu/Zn ratio. We observed significant gender differences in Cu, CER and the Cu/Zn ratio in MDD. Male patients showed a reduced N -acetyl aspartate (NAA)/phosphocreatine + creatine (PCr + Cr) ratio in the left ACC. The NAA/PCr + Cr ratio decreased in the right ACC in patients with MDD. In the left ACC of male MDD patients, the Cu/Zn ratio was inversely related to the NAA/PCr + Cr ratio, and Fe levels were negatively associated with the GPC + PC/PCr + Cr ratio. Our findings highlight gender-specific changes in Cu homeostasis among male MDD patients. The Cu/Zn ratio and Fe levels in male MDD patients were significantly linked to neurometabolic alterations in the ACC. • Serum levels of Cu and CER decreased in men with MDD, while the Cu/Zn ratio increased in women with MDD. • In males with MDD, a gender-specific decrease in the NAA/PCr + Cr ratio was noted in the left ACC. • The Cu/Zn ratio and Fe levels in male MDD patients are associated with changes in neurometabolism in the ACC. [ABSTRACT FROM AUTHOR]

Cassava (Manihot esculenta Crantz) is an important staple crop, especially for resource-poor populations. Cassava-based diets are rich in calories but grossly deficient in essential mineral elements. A field study in southeastern Nigeria aimed at increasing yields and concentrations of bioavailable mineral elements in a β-carotene cassava variety, UMUCASS 38 (TMS 01/1371), through agronomic biofortification. A randomized complete block design experiment in triplicate, with foliar application of Zn (3.0 kg ha−1 Zn as ZnSO4), I (4.0 kg ha−1 I as KI), Se (0.25 kg ha−1 Se as Na2SeO3) and Zn + Se + I in combination at vegetative, tuber initiation and bulking stages of cassava. Results indicated neither phytotoxicity nor altered physical and chemical soil properties. Application of Zn or Zn + Se + I at the vegetative stage significantly increased cassava tuber yields. More Zn, Se, and I accumulated in fresh and processed cassava tuber products (gari, lafun), especially when Zn + Se + I was applied at tuber initiation stage. The combined application of Zn + Se + I consistently outperformed sole applications indicating a synergistic interaction effect between the three elements. It is concluded that in the Zn-deficient soils of southeast Nigeria, yield of cassava root tubers can be significantly increased by foliar application of Zn-containing fertilizer at the vegetative stage, but for optimal accumulation of elemental Zn, Se, and I, a combined Zn + Se + I fertilizer be applied at the tuber initiation stage. [ABSTRACT FROM AUTHOR]

Selenium (Se) is an essential trace element associated with both beneficial and detrimental health effects; deficiency and uncontrolled self-supplementation are both issues of concern. Secondary analysis of a randomized controlled trial and observational studies indicate that Se is a potential risk factor for type 2 diabetes mellitus (T2D). The association between Se and diabetes may be sex specific, with potentially increased risk in men and an inverse association in women, particularly for gestational diabetes mellitus during pregnancy. Recent observational evidence suggests that high Se status favorably affects all-cause mortality risk in patients with manifest diabetes and reduces macrovascular T2D. Selenium (Se) is an essential trace element, which is inserted as selenocysteine (Sec) into selenoproteins during biosynthesis, orchestrating their expression and activity. Se is associated with both beneficial and detrimental health effects; deficient supply or uncontrolled supplementation raises concerns. In particular, Se was associated with an increased incidence of type 2 diabetes (T2D) in a secondary analysis of a randomized controlled trial (RCT). In this review, we discuss the intricate relationship between Se and diabetes and the limitations of the available clinical and experimental studies. Recent evidence points to sexual dimorphism and an association of Se deficiency with gestational diabetes mellitus (GDM). We highlight the emerging evidence linking high Se status with improved prognosis in patients with T2D and lower risk of macrovascular complications. [ABSTRACT FROM AUTHOR]

Purpose: Short stature is prevalent among children worldwide, particularly in developing countries. Various trace elements, including zinc, magnesium, iron, copper, chromium and selenium, are crucial for proper body development. The aim of this study is to explore the relationship between trace elements and TNF-α and IL-6 to elicit and possible pathway responsible for short stature. Methods: Two hundred and twenty samples were recruited for this study, 100 short statures and 120 controls were randomly selected. Six trace elements were measured using graphite furnace atomic absorption spectrometry. The concentrations of IL-6 and TNF-α in serum were assessed utilizing the Enzyme-Linked-Immunosorbent Assay (ELISA). Superoxide dismutase was also analysed to determine the oxidative stress response. Results: The study revealed notable distinctions in serum trace element levels of short stature. They exhibited significant lower levels of zinc and magnesium, alongside higher levels of copper. The altered Cu/Zn ratio seemed to have a positive correlation with short stature. Conversely, no significant disparities were observed in iron, chromium, and selenium levels. Furthermore, a significant rise was noted in proinflammatory marker TNF-α and cytokine IL-6. Additionally, superoxide dismutase was low in the short statures In silico study shows a high affinity of Zinc with TNF alpha. It may be suggested that inflammation at any time during childhood, with the rise in TNF alpha tightly binds with zinc and may have led to a decrease in zinc serum levels, altered redox homeostasis and resulted in short stature. Conclusion: The altered Cu/Zn ratio along with high TNF alpha and IL6 may be used as a marker for short stature in the initial years of growth in children before they reach maturity at the age of 18. Thereafter, introducing zinc supplementation could potentially enhance stature by mitigating TNF-alpha level. Further experimental studies will help to establish the exact role of zinc with TNF alpha in short stature. [ABSTRACT FROM AUTHOR]

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The carbonate rock of the Qiulitage Formation is a significant stratum for oil and gas exploration in the Tarim Basin. To elucidate its environmental characteristics, we conducted tests and analyses of trace elements, carbon, and oxygen isotopes of the carbonate rocks of the Qiulitage Formation in the Kekeqigankake section of the Keping area. The results reveal that δ13C values range between −1.7‰ and 4.3‰, with an average value of 1.645‰. δ18O values fluctuate from −11.4‰ to −6‰, with an average value of −8.2475‰. Z values (paleosalinity) vary from 120.33 to 131.67, significantly exceeding 120 with an average value of 126.52, indicating a marine sedimentary environment. Paleotemperature values (T) range from 12.75 to 29.09 °C, with an average value of 21.36 °C, suggesting warm climate conditions. The Sr/Ba (3.42~24.39) and Sr/Cu (57.5~560) ratios are elevated, while Th/U (<1.32) and V/Cr (0.989~1.70) ratios are reduced, suggesting that the Qiulitage Formation was deposited in an oxygen-rich, warm marine sedimentary environment with relatively high salinity. [ABSTRACT FROM AUTHOR]

The present investigation examined the impact of pomegranate (Punica granatum L.) juice on trace elements, minerals, and oxidative stress in relation to the potential harm inflicted by aluminum chloride (AlCl3) in rats. Rats were split into four groups at random for this purpose: control (C), pomegranate juice (PJ), aluminum chloride (A), and PJ + A. For 30 days, PJ was orally administered by gavage at a rate of 4 mL/kg every other day, whereas AlCl3 was administered intraperitoneally at 8.3 mg/kg. Spectrophotometric analysis was used to measure the levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) enzyme activity in various tissues. In addition, high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS) was used to determine the amounts of the elements Al, Cu, Fe, Mn, Zn, Ca, and Mg in the tissues. It was discovered that when PJ therapy was applied to all tissues, the antioxidant enzymes SOD and CAT activity increased, the GSH level rose, and the MDA level, a sign of lipid peroxidation, decreased. Al and Ca levels increased in the A group relative to the C group in all tissues, whereas they decreased in the A + PJ group relative to the A group. Group A exhibited a proportionate increase in Fe levels in the liver and renal tissues compared with group C. Furthermore, the A group's brain tissue had a higher Fe level than the C group's. The A + PJ group's brain tissue had a lower Fe level than the A group's. Our findings demonstrate that PJ therapy greatly decreased Al buildup and oxidative stress in tissues while controlling variations in trace element levels. In addition, it is concluded that PJ might have value as a strong chelating agent to prevent Al poisoning. [ABSTRACT FROM AUTHOR]

While long-range transport is believed to be the primary source of mercury (Hg) in Antarctica, it is known that seabirds transport organic matter, nutrients, and contaminants from the sea to land. However, these biologically mediated inputs have not been widely evaluated. Anthropogenic contamination and natural occurrence have increased Hg in the environment. Antarctic seabirds, such as penguins and shags, form dense colonies and may provide an important local source of Hg input. This study determined the concentrations of total mercury (THg) in ornithogenic soils associated with eight species of seabirds on King George Island (Antarctica Peninsula) and evaluated species-specific differences. Soils from colonies of Southern Giant Petrel (Macronectes giganteus; mean ± SD, 213 ± 132 ng g−1) and Imperial Shag (Leucocarbo atriceps; 144 ± 54 ng g−1) had significantly higher THg concentrations than non-ornithogenically influenced (29 ± 35 ng g−1) soil samples. Soils associated with Southern Giant Petrels also had significantly higher THg than those of Kelp Gull (Larus dominicanus; mean 17 ± 15 ng g−1), which we speculate is a result of petrels occupying a higher trophic position in the marine food web, which leads to a greater accumulation of Hg in their organism, then being excreted through guano. Moreover, a moderate relationship observed between THg-Cd and THg-SOM suggests an influence of those species. Our data confirm that Antarctic seabird colonies are associated with higher soil THg concentrations and report the higher concentration for ornithogenically influenced soil (508 ng g−1) in a level similar to an anthropogenic site and highlight the need for a more thorough evaluation. [ABSTRACT FROM AUTHOR]

Aerosols continuously transport trace elements (TEs) across long distances to the ocean, fueling marine primary production and affecting global carbon cycles. Given the multiple sources and complex transport mechanisms, field investigations of aerosol TEs on a global scale are significant for understanding their role in marine biogeochemical cycles. Here, aerosol samples were collected along a 50,000‐km route covering subtropical Northwest Pacific (NWP) marginal seas, Indian Ocean, Southern Ocean, Drake Passage, and South Pacific. Samples were analyzed for the concentrations of Al, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, Tl, and Pb. Aerosol TEs were distributed heterogeneously, with significantly lower concentrations over remote oceans compared to coastal seas. Meanwhile, TE concentrations were generally high in the Indian Ocean, moderate in the Southern Ocean, and low in the South Pacific. Cr, Ni, Cu, Zn, Cd, As, and Pb were widely enriched, primarily originating from anthropogenic sources, while Al, Ti, V, Mn, Fe, and Co were mainly from crustal sources in remote oceans. Moreover, specific sources of TEs were clarified, for example, Cr and Ni were mainly from vehicle emissions. The estimated bulk TE deposition fluxes also varied spatially. For instance, the greatest deposition of Fe occurs in the NWP marginal sea, followed by the Drake Passage, Indian Ocean Sector of Southern Ocean, Pacific Sector of Southern Ocean, and South Pacific. This study contributes to a deeper understanding of the complex dynamics of aerosol TEs in the global ocean, providing valuable information for future studies and policy making regarding climate change. Plain Language Summary: Trace Elements serving as essential micronutrients (e.g., Fe and Mn), harmful pollutants (e.g., As and Pb), or reliable tracers (e.g., Ti and Al) are crucial for understanding marine biogeochemical processes. Aerosols continuously transport TEs over long‐distances to the ocean, providing a significant source to the surface layer. Their complex sources and transport mechanisms cause substantial spatial variability in distribution and deposition. Limited observations and challenges of uncontaminated sampling further constrain our understanding of the role of aerosol TEs in the ocean. During a 180‐day cruise, aerosol TEs were observed across a large spatial scale, providing an opportunity to better explore differences in aerosol TEs across multiple ocean basins due to consistent sampling and analysis. Results showed that TE concentrations were generally high in the Indian Ocean, moderate in the Southern Ocean, and low in the South Pacific. We identified TEs from crustal and anthropogenic sources, and further elucidated the specific origins of the anthropogenic elements. Furthermore, the bulk deposition fluxes of 14 TEs and dust as well as the contributions of dry and wet deposition. These findings help elucidate the aerosol TE depositions and their influence on marine TE cycles. Key Points: Field observations of aerosol trace elements are conducted in the NW Pacific marginal sea, Indian Ocean, Southern Ocean, and South PacificThe degree of enrichment and the specific sources of trace elements are presentedBulk fluxes of dust and trace element as well as contribution ratios of dry and wet deposition are estimated across multiple basins [ABSTRACT FROM AUTHOR]

This article reviews the present condition and future possibilities of walnut growing in the Republic of Uzbekistan. It is stated that the recent decrees and resolutions of the President of the Republic of Uzbekistan and the Government have led to the expansion of the area dedicated to fruit crops, particularly walnuts. The establishment of the "Association of Producers and Exporters of Walnuts" has enabled the creation of modern plantations in Uzbekistan consisting of high-yielding walnut varieties that are well-suited to the local natural and climatic conditions. The association also aims to promote the adoption of science-based methods and resource-saving technologies for walnut growing. Furthermore, it aims to increase walnut production in different regions of the Republic, conduct market research to expand the sales market, develop strategies to boost walnut exports and carry out research to implement innovative technologies in walnut production processes. Since one of the products of walnuts in Uzbekistan is walnut jam, the paper also presents the results of experimental studies on the chemical composition of jam made from unripe walnuts. The studies show that the primary macronutrients in unripe walnut jam are sodium, potassium, magnesium, calcium, and iron. Additionally, it contains trace elements such as iodine, zinc, cadmium, nickel, and cobalt. Due to the diverse macro- and micro-elemental composition of unripe walnut jam, this product can be used as both a food item and a preventive material against various diseases, particularly iodine deficiency in the human body.

Abstract Background Gallstones are a common digestive disorder that threatens human health. Iron deficiency may be related to the formation of gallstones, but there is limited current epidemiological research. The objective of this study was to investigate the relationship between iron status and gallstones. Methods The datasets from the National Health and Nutrition Examination Survey (NHANES) 2017–2020 were used in a cross-sectional investigation. Gallstones were determined by using the 2007–2010 NHANES questionnaire. Multivariate linear regression models were used to examine the association between serum iron, serum ferritin and iron intake with the risk for gallstones. Subgroup analysis based on gender, age, race, and diabetes were performed. Fitted smoothing curves were used to describe the linear relationship. Results The research involved 7847 participants aged 20 and above, among whom 845 were identified as having gallstones. Participants with higher serum iron levels tended to have a lower gallstones prevalence. A negative relationship between serum iron and gallstones prevalence was observed (OR = 0.979, 95% CI:0.965–0.992). The group with the highest serum iron tertile had a 23.7% lower risk of gallstones compared to the lowest tertile (OR = 0.763, 95% CI:0.628‒0.929). Gallstone prevalence was inversely correlated with iron intake in model 1. The negative association between serum iron and gallstones remained stable in stratifications, including gender, age, race, and diabetes. Conclusions Elevated serum iron was associated with a decreased prevalence of gallstones. However, to confirm the impact of long-term iron metabolism on gallstone formation, additional prospective research is necessary.

Abstract Trace elements released into the atmosphere by human activities, such as fossil fuel combustion, flow into forest ecosystems via wet and dry deposition and can flow out via mountainous stream water. The Kajikawa catchment site in Niigata Prefecture, located on the Sea of Japan side in central Japan, suffers from large amounts of acidic substance deposition due to its geographical conditions and meteorological seasonality and is affected by transboundary air pollution originating from the Asian continent due to northwesterly seasonal winds in winter. This study determined the concentrations of trace elements dissolved in precipitation and stream water to comprehensively evaluate the effects of transboundary air pollution on forest ecosystems. The results showed that the concentrations of trace elements and major inorganic ions in precipitation tended to be high in winter and low in summer. The fluxes of many trace elements also increased in winter, reflecting high precipitation amount. Except for Sr, the enrichment factors of the highly enriched trace elements did not show clear seasonality. Therefore, they were continuously influenced by anthropogenic activities. Furthermore, the stable Pb isotope ratio changed significantly during the observation period; however, this was not explained by seasonal changes. This study revealed that trace elements are transported to the Sea of Japan side in central Japan, especially in winter; however, their quantity and content change in response to changes in local and transboundary air pollution. Graphical Abstract

Ashes from biomass combustion (BAs) are waste materials that contain valuable nutrients, making them suitable for reuse in plant fertilization. The application of ash positively impacts soil properties, including the reduction of excessive acidification, which enhances nutrient absorption. However, the use of ash can also introduce potentially toxic elements into the soil in excessive amounts. This study analyzed the effect of different doses of BAs on the accumulation of zinc (Zn), copper (Cu), nickel (Ni), chromium (Cr), lead (Pb), and cadmium (Cd) in the surface layer of Haplic Luvisol soil and in various parts of spring barley plants. The research was conducted over three years under field experiment conditions. Throughout the three-year study period, no significant changes were observed in the content of individual elements in the soil or in various parts of the spring barley plants. The analyzed elements tended to accumulate more in the roots of the plants than in their above-ground parts. When considering the average content of individual elements for the entire study period, no significant increase was noted in their levels within the analyzed soil. However, when examining each year of the experiment separately, significant dynamics in the content of individual elements in both plants and soil were identified. These fluctuations were likely influenced by variations in weather conditions, as the highest concentrations of the analyzed elements were typically recorded in the second year of the study, which coincided with the highest total rainfall of 792.4 mm. The findings indicate that analyzing changes in the content of elements introduced via biomass combustion ashes necessitates comprehensive studies conducted under both controlled and real-world conditions. Without such thorough investigations, conclusions regarding the release of components contained in BAs, particularly concerning their potential toxicity, may be misleading.

Abstract Background Drug-resistant epilepsy is defined as failure of seizure control in spite of using 2 or 3 proper antiepileptic drugs in appropriate time. Mineral elements play important roles in neuronal function; it is believed that mineral deficiency may lead to complications through seizure management. In the present study, serum levels of zinc (Zn), copper (Cu), magnesium (Mg), calcium (Ca), and 25-hydroxy vitamin D (Vit D) in drug-resistant-epilepsy (DRE) patients were evaluated and compared with the controlled patients. Methods In this cross-sectional study, epileptic patients were included and categorized into two groups of DRE and well-controlled patients. Patients’ serum samples were analysed to evaluate Zn, Cu, Mg, Ca, and Vit D levels. The primary objective was comparison of serum levels of different trace elements between the groups. Results Sixty-four epileptic children including 33 DRE and 31 well-controlled children entered the study. The DRE children showed a significantly earlier onset of disease compared to the other group (p = 0.014). Comparing the frequency of developmental delay between the groups, the results showed this complication was significantly more frequent in the DRE group (p 0.05). Conclusion The results of the present study delineated that drug-resistant epilepsy patients had earlier onset of disease and were at higher risk of neurodevelopmental delay compared with well-controlled-epilepsy patients. A significant lower serum levels of Zn were also observed in drug-resistant-epilepsy patients. This finding may suggest the role of zinc supplementation in help to better control of drug-resistant seizures, as well as, the importance of serum zinc monitoring in epileptic patients.

Abstract Maize (Zea mays L.), a staple food and significant economic crop, is enriched with riboflavin, micronutrients and other compounds that are beneficial for human health. As emphasis on the nutritional quality of crops increases maize research has expanded to focus on both yield and quality. This study exploreed the genetic factors influencing micronutrient levels in maize kernels through a comprehensive genome-wide association study (GWAS). We utilized a diverse panel of 244 inbred maize lines and approximately 3 million single nucleotide polymorphisms (SNPs) to investigate the accumulation of essential and trace elements including cadmium (Cd), cobalt (Co), copper (Cu), nickel (Ni), selenium (Se) and zinc (Zn). Our analysis identified 842 quantitative trait loci (QTLs), with 12 QTLs shared across multiple elements and pinpointed 524 potential genes within a 100 kb radius of these QTLs. Notably ZmHMA3 has emerged as a key candidate gene previously reported to influence the Cd accumulation. We highlighted ten pivotal genes associated with trace element transport including those encoding heavy metal ATPases, MYB transcription factors, ABC transporters and other crucial proteins involved in metal handling. Additionally, haplotype analysis revealed that eight inbred linesaccumulated relatively high levels of beneficial elements while harmful elements were minimized. These findings elucidate the genetic mechanisms underlying trace element accumulation in maize kernels and provide a foundation for the breeding of nutritionally enhanced maize varieties.

A method of quadrupole inductively coupled plasma mass spectrometry (Q-ICP-MS) based on oxygen reaction mode was established for the accurate determination of trace impurity elements in superalloys. The effect of the collision/reaction mode on the removal of mass spectral interference was investigated. By contrast, the polyatomic mass spectral interference on the target element Cd can be effectively removed under oxygen dynamic reaction cell (DRC-O2) mode. The signal-to-noise ratio (SNR) peaked when the gas flow was 2 mL/min, and the detection limit was approximately three orders of magnitude lower than that under standard (STD) mode. In addition, the elimination mechanism of mass spectrometric interference was studied. It was found that for the element X (X=Rb, Nb, Mo, Cs, Ta, W or U), the conversion of the interferent species XO to the non-interferent species XOO is a spontaneous reaction when the bond energy of X−O bond is higher than that of O−O bond. Therefore, it is easier to generate ions of XOO species when there is adequate O2 present in the ion source, so as to eliminate XO interference. Based on these results, experiments were carried out to determine elements of Rb, Nb, Mo, Cs, Ta, W and U. The matrix simulated solutions containing high concentration of Mo, Nb, Ta, and W were used to evaluate the methodological parameters for the measurement of trace Cd, Ag, Au, and Hg impurities, respectively. The limit of detection (LOD) of the trance elements is 0.08-0.31 μg/g, the relative standard deviation (RSD) of the measurement is 2.3%-3.1% (n=6), and the spiked recovery is in the range of 98%-103%. The reliability of the method was further verified by certified superalloy reference materials. Finally, the trace impurities in three types of superalloy samples were measured. The results indicated that the proposed method can achieve rapid, reliable analysis with low detection limit. Besides, it can meet the measurement requirement of trace impurities when the main elements of Mo, Nb, Ta and W exist in superalloy.

This study investigates the effect of minor Li addition on the solute clustering and thermal stability of precipitates in artificially aged Al–Mg–Si–Cu (AA6061) alloys. Li addition hinders the initial changes in hardness and electrical conductivity by delaying solute clustering during early aging. The delay in cluster formation is attributed to the disturbance of Mg enrichment by the formation of Si–Li clusters, unlike the Sn element with vacancy-trapping effect. Additionally, Li addition is found to coarsen the precipitates during over aging, thereby reducing the thermal stability of the Al–Mg–Si–Cu alloy. Gibbsian interfacial excess calculations of the Li solute indicate that the low coarsening resistance of the Li-containing precipitate is mainly caused by the small amount of equilibrium Li segregation at the internal interface between the matrix and over-aged precipitate. A decrease in the number of nucleation sites, originating from the suppression of cluster formation in the early-aging stage, is also suggested as a reason for the coarse precipitate structure. Finally, despite the high solubility of Li into precipitates, Li addition does not disorder or change the crystal structure of the precipitates. It simply increases the volume of the precipitate by an amount equivalent to or greater than the added Li, without increasing the thermal stability.

The deposition of metallic substances on farmland is of critical importance owing to its possible detrimental impacts on the surroundings and human wellness. Heavy metals can adversely affect the physicochemical properties of soil and plant health. Prolonged exposure to heavy metals in humans can lead to both carcinogenic and non-carcinogenic effects. The present research intended to determine the dispersion condition of toxic metals on cropland and assess the attendant environmental and health dangers in the Wonosobo Regency. This study used a soil sample survey encompassing 312 soil sampling sites. The completed analyses comprise geographical analysis, index of geo-accumulation (IG), contamination index (CI), Nemerow comprehensive contamination index (NCCI), risk analysis, and health risk analysis. The research findings reveal that cadmium material is the primary contributor to contamination from heavy metals in farmland in Wonosobo Regency, as shown by IG analysis. The CI and environmental hazards assessments suggest considerable pollution levels. Kids are more prone to illnesses than adults due to their daily intake amount, so it is necessary to pay more attention to the daily intake sources in children to minimize exposure to heavy metals.

The purpose of this research was to establish the baseline concentrations of thirteen trace elements (Cd, Co, Cr, Cu, Ni, Pb, Fe, Mg, Na, K, Sr, Ca, and Zn) in feathers of the common blackbird Turdus merula (n=9) at the Natura 2000 site “Marine Dunes from Agigea” (ROSCI0073). Both remiges and rectrices were pooled, and the quantification of trace elements was accomplished using two spectrometry analysis methods: high-resolution continuum source atomic absorption spectrometer with a graphite furnace (HR-CS GF-AAS) and flame atomic absorption spectrometer (FL-AAS). The concentration of Ca was the highest of the thirteen trace elements, registering at 209.9146±192.4098 µg·g-1 w.w., while Cd had the lowest concentration levels in feathers, 0.0243±0.0158 µg·g-1 w.w., respectively. However, the results obtained were significantly lower than those in the relevant literature. These findings suggest that the bioavailability of the analysed trace elements is minimal in our study area. Thus, it is imperative to conduct further research on the impact of these trace elements' accumulation and effects on avifauna in the Natura 2000 site “Marine Dunes from Agigea”.

Background: Drug-resistant epilepsy is defined as failure of seizure control in spite of using 2 or 3 proper antiepileptic drugs in appropriate time. Mineral elements play important roles in neuronal function; it is believed that mineral deficiency may lead to complications through seizure management. In the present study, serum levels of zinc (Zn), copper (Cu), magnesium (Mg), calcium (Ca), and 25-hydroxy vitamin D (Vit D) in drug-resistant-epilepsy (DRE) patients were evaluated and compared with the controlled patients. Methods: In this cross-sectional study, epileptic patients were included and categorized into two groups of DRE and well-controlled patients. Patients' serum samples were analysed to evaluate Zn, Cu, Mg, Ca, and Vit D levels. The primary objective was comparison of serum levels of different trace elements between the groups. Results: Sixty-four epileptic children including 33 DRE and 31 well-controlled children entered the study. The DRE children showed a significantly earlier onset of disease compared to the other group (p = 0.014). Comparing the frequency of developmental delay between the groups, the results showed this complication was significantly more frequent in the DRE group (p < 0.001). Concerning serum elements, the results showed a significantly higher concentration of Zn in the well-controlled group than the DRE group (p = 0.007). On the other hand, no significant differences were observed between the groups regarding the means of Vit D, Ca, Cu, and Mg levels (p > 0.05). Conclusion: The results of the present study delineated that drug-resistant epilepsy patients had earlier onset of disease and were at higher risk of neurodevelopmental delay compared with well-controlled-epilepsy patients. A significant lower serum levels of Zn were also observed in drug-resistant-epilepsy patients. This finding may suggest the role of zinc supplementation in help to better control of drug-resistant seizures, as well as, the importance of serum zinc monitoring in epileptic patients. [ABSTRACT FROM AUTHOR]

Offshore volcanic activity occurs when an underwater volcano erupts. These volcanoes can be located underwater or on land and can be very dangerous to marine life. The aim of this study is to examine whether the influence of two volcanoes has an effect on the concentrations of metals and trace elements in the Atlantic mackerel Scomber colias in the Canary Islands. For the study, ten specimens of S. colias were obtained from each sampling area in March 2022. Metal concentrations were determined by inductively coupled plasma optical emission spectrophotometry (ICP-OES). The specimens of Scomber colias from the area affected by the Tajogaite volcano (La Palma) showed higher concentrations of all metals and trace elements analyzed than those from the other study areas. The specimens from the area of influence of the Tagoro Volcano (El Hierro) showed the second highest concentration of the elements studied, although this volcanic process is in a state of degassing. [ABSTRACT FROM AUTHOR]

The crude oils of the Sarvak reservoir were studied by integrated geochemical, inorganic and isotopic analyses to evaluate the origin, depositional conditions, geological age, thermal maturity of the source rocks and possible facies from which these oils were sourced. This study provides new insights into the Middle-Jurassic age source rock in the Azar Oilfield. This is the first geochemical study in Azar Oilfield where nonbiomarker parameters and biomarker parameters were utilized to achieve the objectives. The n-alkane distribution pattern along with their standard ratios, including CPI (0.83–1.03), TAR (0.18–0.29) and isoprenoids (Pr/Ph, 0.52–0.65) as well as pristane/n-C17 versus phytane/n-C18 cross-plot indicate a marine source of the organic matter deposited in an anoxic condition. The sterane parameters such as C27 and C29 are characterized by the predominance of C27ααα-20R steranes (41%–49%) and also depict the algal source of organic matter. The organic input and facies of the source units were also determined by terpanes C29/C30H, Ts/Tm, C35/C34-HH, and DBT/Phen. The relatively high ratio of C29/C30H along with the ratios of Ts/Tm (<0.5) and C35/C34 (>0.8) reflect the carbonate marine facies of the source rocks. Furthermore, the higher values of the homohopane index (>0.1) along with the low ratio of the gammacerane/C30-H (0.06–0.22) as well as the high ratio of V/Ni (>1) further indicate anoxic environments. The dibenzothiophene/phenanthrene ratios of the oil samples (from 2.43 to 3.25) indicate the marine carbonates/marl zone. This genetic classification is also supported by stable carbon isotopic compositions (δ13C). Most of the maturity-related biomarkers and nonbiomarker parameters such as CPI, steranes-C29S/(S + R), ββ/(αα+ββ), moretane to hopane (M29/C30H), pentacyclic terpanes C27Ts/ (Ts + Tm), C32-S/(S + R) hopanes, and methyl phenanthrene index agree that the analyzed oils have originated from mature source rocks. Ultimately, this study has demonstrated that analyses of biomarkers and their stable isotope compositions (δ13C and δ34S) complemented with trace element data provide an excellent novel tool for better understanding the basic concepts in petroleum basins and for solving a wide range of problems during petroleum exploration. [ABSTRACT FROM AUTHOR]

Trace elements are the essential mineral nutrients in grassland, however, we still know little about the distributions of trace elements in grassland with long‐term grazing exclusion. The contents, stocks, and proportions of iron (Fe), aluminum (Al), manganese (Mn), and boron (B) in green plant‐litter‐root‐soil were evaluated by enclosing for 18, and 39 years inside the fence (F18 and F39) and grazing outside the fence (F0) in Inner Mongolia grassland. The results showed that F18 and F39 decreased the stocks of Fe, Al, and Mn in green plant and root compared to F0 (p <.05), while increased the stocks of them in litter (p <.05). The stock of Fe, Al, and Mn in green plant at F39 was 28.6%, 13.9%, and 39.2% higher than that at F18. The stocks of four trace elements in first layer litter at F39 were increased by 12.7%–52.2% compared to F18, whereas the stocks of them in third layer litter were decreased by 32.2%–42.5%. The F18 obviously increased the stocks of Fe and Mn in soil, especially B (p <.05). While the stocks of these trace elements in soil at F39 were 9.1%–28.0% lower than that at F18, especially B (p <.05). In conclusion, the trace elements were mainly shifted from green plant and root to soil and third layer litter with 18‐year grazing exclusion. Compared to 18‐year grazing exclusion, the trace elements were shifted from third layer litter and soil to root with 39‐year grazing exclusion. [ABSTRACT FROM AUTHOR]

Today, one of the most important problems for humans is disease and mortality due to air pollutants, especially in large and industrial cities. This research is to evaluate the health risk of children and adults' exposure to heavy metals and PAHs in the air of cities of Arak, Isfahan, Ahvaz, Tabriz, Shiraz, Karaj and Mashhad and identifying the factors affecting pollution, based on sampling data from 120 stations, was done during 2019–2020. ICP OES and GC/MS were used to determine the amount of heavy metals and PAHs, respectively. US-EPA models were used for carcinogenic and non-carcinogenic risk assessment and Factor analysis models, PCA, CFA with SPSS and LISREL software were used to identify factors affecting contamination. The risk index for children and adults, except in the cities of Arak and Isfahan, was obtained within the allowable range and the carcinogenicity and non-carcinogenicity risk of all metals, except Mn in Arak and Isfahan for children and adults was seen below the threshold. The carcinogenic risk of all PAHs was also obtained to an acceptable extent. During the statistical analysis, combustion of fuels, vehicle exhaust, tyre wear and industrial activities were identified as the most important factors in causing pollution. [ABSTRACT FROM AUTHOR]

The Bou Dahar Pb-Zn district, located in the Moroccan High Atlas, is a typical carbonate-hosted Pb-Zn ore district (>30 Mt at 4 wt.% Pb, 4 wt.% Zn). In situ trace element analysis was performed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) on galena and cerussite from different ore types. The galena is generally enriched in Ag and Sb, secondarily enriched in Cu, with a trace amount of Cd and As, but extremely depleted in Bi and Tl. The main substitution mechanism in galena is (Ag, Cu)+ + Sb3+ ↔ 2Pb2+, and at high Sb concentrations, the further substitution of 2Sb3+ + □ ↔ 3Pb2+ (where □ represents a vacancy) took place. Micro-inclusions of Cu-Sb-bearing minerals (such as tetrahedrite) and Ag-bearing minerals (such as acanthite) may exist in some situations. The features of trace elements in galena show the existence of different coupled substitutions in vein-related ore, breccia-related ore, and strata-bound ore. This suggests that the Bou Dahar district experienced multistage mineralization. The MVT model alone cannot fully explain the ore-forming process. The cerussite replacing strata-bound galena is enriched in Sr, Ba, Ag, and Cu, with minor Sb, As, and Tl. Strontium and Ba are directly substituted with Pb in the cerussite lattice. Copper and Ag are likely present in cerussite as nano-inclusions, which differs from the coupled substitution mechanism of the original galena. High concentrations of Ag may occur due to minor electrum inclusions. The enrichment of Ag, Cu, and Au in cerussite during the oxidation process may guide the optimization of ore processing, especially in extracting valuable trace/minor elements. [ABSTRACT FROM AUTHOR]

Simple Summary: Supplementing trace minerals is common for managing bovine respiratory disease in developing beef cattle; however, its effects on their immune and metabolic systems are not fully understood. In this study, we evaluated three different mineral supplement programs and assessed their impact on the concentrations of copper, manganese, cobalt, and zinc, along with the whole blood gene expression, in high-risk beef cattle that remained clinically healthy over a 60-day period. Our results demonstrated that one supplement program, which included amino acid (organically) complexed minerals, led to an increase in gene activity related to adaptive immune system function and the metabolism of carbohydrates and fat-soluble vitamins compared to cattle-fed sulfate (inorganically) sourced complexed minerals. Additionally, the cattle given organically sourced minerals resulted in higher liver concentrations of copper at the end of the study compared to cattle given inorganically sourced minerals. This suggests that tailored mineral supplement programs may improve cattle immunity and mineral absorption. Understanding these effects improves how we manage cattle for diseases such as respiratory disease. Supplementing trace minerals is common in managing bovine respiratory disease (BRD) in post-weaned cattle; however, its influence on host immunity and metabolism in high-risk cattle remains unclear. We aimed to assess the impact of three supplementation programs on liver and serum trace element concentrations and blood gene expression. Fifty-six high-risk beef steers were randomly assigned to one of three groups over 60 days: (1) sulfate-sourced Cu, Co, Mn, and Zn (INR), (2) amino acid-complexed Cu, Mn, Co, and Zn (AAC), or (3) AAC plus trace mineral and vitamin drench (COMBO). Serum and liver biopsies for Cu, Co, Mn, and Zn at d0, d28, and d60 were analyzed from cattle free of BRD (n = 9 INR; n = 6 AAC; n = 10 COMBO). Differences and correlations of mineral concentrations were analyzed via generalized linear mixed models and Spearman's rank coefficients, respectively (p < 0.05). Whole blood RNA samples from healthy cattle (n = 4 INR; n = 4 AAC; n = 4 COMBO) at d0, d13, d28, d45, and d60 were sequenced and analyzed for differentially expressed genes (DEGs) via glmmSeq (FDR < 0.05), edgeR (FDR < 0.10), and Trendy (p < 0.10). Serum and liver Cu and Co concentrations increased over time in all groups, with higher liver Cu in COMBO (487.985 μg/g) versus AAC (392.043 μg/g) at d60 (p = 0.013). Serum and liver Cu concentrations (ρ = 0.579, p = 6.59 × 10−8) and serum and liver Co concentrations (ρ = 0.466, p = 2.80 × 10−5) were linearly correlated. Minimal gene expression differences were found between AAC versus COMBO (n = 2 DEGs) and INR versus COMBO (n = 0 DEGs) over time. AAC versus INR revealed 107 DEGs (d13–d60) with increased traits in AAC including metabolism of carbohydrates/fat-soluble vitamins, antigen presentation, ATPase activity, and B- and T-cell activation, while osteoclast differentiation and neutrophil degranulation decreased in AAC compared to INR. Our study identifies gene expression differences in high-risk cattle fed inorganic or amino acid-complexed mineral supplements, revealing adaptive immune and metabolic mechanisms that may be improved by organically sourced supplementation. [ABSTRACT FROM AUTHOR]

Copper, a micronutrient in low concentrations, in excess causes negative changes in the growth and development of plants. This study advances understanding in the dynamics of potentially toxic elements in areas with different uses, making it possible to evaluate the potential of C. cajan to remedy/reduce excess Cu in the soil. It was observed that in C. cajan grown in agricultural soil with high P availability, the translocation of Cu to shoot was 5−7 times greater than in grassland and grassland contaminated with Cu, where the preferred allocation for Cu was the root. The efficiency of the photosynthetic and symbiotic system was not affected; but an increase in the concentration of amino acids was observed. Because of considerable accumulation of Cu in the root and shoot, C. cajan was tolerant to a dose of approximately 100 mg kg−1 of Cu in viticulture soil and may be indicated as a phyto-extractor agent for this metal in agricultural soils. [ABSTRACT FROM AUTHOR]

Molybdenum (Mo), an essential mineral, plays a key role in the vital activity of the organism. However, excess Mo in the forage will cause loss of appetite, diarrhea, emaciation, bone injury, joint abnormalities, and anemia in animals. In order to study molybdenosis in the Procapra picticaudata in the animal rescue center, samples of soils, forages, blood, and liver were collected. The mineral contents of all samples were determined, and the blood parameters were also measured. The results showed that the Mo level in the soil and forage in the animal rescue center was significantly higher than that in healthy pastures (p < 0.01). The Mo concentrations in the blood and liver in the P. picticaudata from the animal rescue center were also noticeably higher than those in healthy animals (p < 0.01). The level of Cu in the blood and liver were noticeably lower than those in healthy P. picticaudata (p < 0.01). The superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), and catalase (CAT) were significantly lower than those in healthy animals. Supplementing copper sulfate (CuSO4) could significantly decrease the Mo content in the blood, and cure molybdenosis. In summary, the excessive Mo content in the soil and forage in the animal rescue center had greatly affected physiological parameters and antioxidant capacity. It is likely that the molybdenosis of the P. picticaudata is caused by the high Mo contents in soils and forages. CuSO4 may alleviate molybdenosis in P. picticaudata. [ABSTRACT FROM AUTHOR]

The Malysheva emerald mine (Urals, Russia) boasts a long history and extraordinary emerald output. However, recent studies indicate that Malysheva emeralds share highly similar inclusion varieties, UV-visible-near infrared (UV-Vis-NIR) spectra, and compositional characteristics with other tectonic-magmatic-related (type I) emeralds from Zambia, Brazil, and Ethiopia. This similarity poses challenges for determination of the emeralds' origin. This paper systematically investigates the microscopy, spectroscopy, and trace element chemistry of Malysheva emerald samples and compiles previously reported compositional data for the aforementioned Type I emeralds. Based on this dataset, principal component analysis (PCA) and machine learning methods are employed to construct models for emerald provenance discrimination. The results have updated the provenance characteristics of Malysheva emeralds, confirming the solid phase component of their three-phase inclusions as siderite and revealing two UV-Vis-NIR spectral patterns. Furthermore, the unique infrared absorptions related to HDO and D2O molecules within the 2600–2830 cm−1 range were discovered, which can be indicative of the origin of Malysheva. The prediction results of the machine learning model demonstrate an accuracy rate of 98.7%, and for an independent validation set of Malysheva emeralds, the prediction accuracy reached 100%. The feature importance ranking of the model highlights trace elements and parameters strongly correlated with the emeralds' origin. These results illustrate the enormous potential of machine learning in the field of emerald origin determination, offering new insights into the traceability of precious gemstones. [ABSTRACT FROM AUTHOR]

The impact of introducing trace transition elements on the thermal stability and conductivity of pure copper was examined through metallographic microscopy (OM), transmission electron microscopy (TEM), and electrical conductivity measurements; the interaction between trace transition element and trace impurity element S in the matrix was analyzed. The results show that the addition of trace Ti and trace Cr, Ni, and Ag elements significantly enhances the thermal stability of the pure copper grain size. After high-temperature treatment at 900 °C/30 min, the grain sizes of Cu, Cu-Ti-S, and Cu-Cr-Ni-Ag-S were measured and found to be 200.24 μm, 83.83 μm, and 31.08 μm, respectively, thus establishing a thermal stability ranking of Cu-Cr-Ni-Ag-S > Cu-Ti-S > Cu. Furthermore, the conductivities of pure copper remain high even after the addition of trace transition elements, with recorded values for Cu, Cu-Ti-S, and Cu-Cr-Ni-Ag-S of 100.7% IACS, 100.2% IACS, and 98.5% IACS, respectively. The enhancement of thermal stability is primarily attributed to the pinning effect of the TiS and CrS phases, as well as the solid solution dragging of Ni and Ag elements. Trace Ti and Cr elements can react with S impurities to form a hexagonal-structure TiS phase and monoclinic-structure CrS phase, which are non-coherent with the matrix. Notably, the CrS phase is smaller than the TiS phase. In addition, the precipitation of these compounds also reduces the scattering of free electrons by solute atoms, thereby minimizing their impact on the alloy's conductivity. [ABSTRACT FROM AUTHOR]

Maize (Zea mays L.), a staple food and significant economic crop, is enriched with riboflavin, micronutrients and other compounds that are beneficial for human health. As emphasis on the nutritional quality of crops increases maize research has expanded to focus on both yield and quality. This study exploreed the genetic factors influencing micronutrient levels in maize kernels through a comprehensive genome-wide association study (GWAS). We utilized a diverse panel of 244 inbred maize lines and approximately 3 million single nucleotide polymorphisms (SNPs) to investigate the accumulation of essential and trace elements including cadmium (Cd), cobalt (Co), copper (Cu), nickel (Ni), selenium (Se) and zinc (Zn). Our analysis identified 842 quantitative trait loci (QTLs), with 12 QTLs shared across multiple elements and pinpointed 524 potential genes within a 100 kb radius of these QTLs. Notably ZmHMA3 has emerged as a key candidate gene previously reported to influence the Cd accumulation. We highlighted ten pivotal genes associated with trace element transport including those encoding heavy metal ATPases, MYB transcription factors, ABC transporters and other crucial proteins involved in metal handling. Additionally, haplotype analysis revealed that eight inbred linesaccumulated relatively high levels of beneficial elements while harmful elements were minimized. These findings elucidate the genetic mechanisms underlying trace element accumulation in maize kernels and provide a foundation for the breeding of nutritionally enhanced maize varieties. [ABSTRACT FROM AUTHOR]

The goal of this study was to evaluate the trace element pollution in amarine ranching area in China based on molecular responses (expression of metallothionein and antioxidant enzyme genes), and biochemical biomarkers (metallothionein content, antioxidant enzyme activities, and malonaldehyde level) in four indicator species. We collected samples of two fish (Lateolabrax maculatus and Sebastes schlegelii), one crustacean (Charybdis japonica), andonegastropod(Rapana venosa) fromthewestern Furong Island marine ranching area and from an adjacent area in March 2022 and measuredthe traceelementcontent in these indicator species aswell as intheseawater andsediment.Wefoundthat thebioaccumulationcharacteristicsof traceelementsand the response patterns of biomarkers were species specific. Moreover, not every biomarker was significantly correlated with environmental trace element content.We then established two biomarker combinations indicative of trace element pollution in seawaterandsediment, respectively, basedonthe correlationbetweenbiomarkers and trace element contents. The selected biomarkers were integrated using integrated biomarker response version 2 (IBRv2). IBRv2 values in the studied marine ranching area were lower than those in the adjacent area. Additionally, these values were consistent with thebioaccumulation of traceelementsintheindicator species, theintegrated trace element pollution index for seawater, and the potential risk index for sediment. These results show that this multi-biomarker and multi-species IBRv2 approach provided a comprehensive diagnosis of trace element pollution in the marine ranching area. Therefore, its application may be beneficial for marine environmental monitoring and management in view of the ecotoxicological impact of pollutants on organisms. [ABSTRACT FROM AUTHOR]

A method of quadrupole inductively coupled plasma mass spectrometry (Q-ICP-MS) based on oxygen reaction mode was established for the accurate determination of trace impurity elements in superalloys. The effect of the collision/reaction mode on the removal of mass spectral interference was investigated. By contrast, the polyatomic mass spectral interference on the target element Cd can be effectively removed under oxygen dynamic reaction cell (DRC-O2) mode. The signal-to-noise ratio (SNR) peaked when the gas flow was 2 mL/min, and the detection limit was approximately three orders of magnitude lower than that under standard (STD) mode. In addition, the elimination mechanism of mass spectrometric interference was studied. It was found that for the element X (X=Rb, Nb, Mo, Cs, Ta, W or U), the conversion of the interferent species XO to the non-interferent species XOO is a spontaneous reaction when the bond energy of X−O bond is higher than that of O−O bond. Therefore, it is easier to generate ions of XOO species when there is adequate O2 present in the ion source, so as to eliminate XO interference. Based on these results, experiments were carried out to determine elements of Rb, Nb, Mo, Cs, Ta, W and U. The matrix simulated solutions containing high concentration of Mo, Nb, Ta, and W were used to evaluate the methodological parameters for the measurement of trace Cd, Ag, Au, and Hg impurities, respectively. The limit of detection (LOD) of the trance elements is 0.08-0.31 μg/g, the relative standard deviation (RSD) of the measurement is 2.3%-3.1% (n=6), and the spiked recovery is in the range of 98%-103%. The reliability of the method was further verified by certified superalloy reference materials. Finally, the trace impurities in three types of superalloy samples were measured. The results indicated that the proposed method can achieve rapid, reliable analysis with low detection limit. Besides, it can meet the measurement requirement of trace impurities when the main elements of Mo, Nb, Ta and W exist in superalloy. [ABSTRACT FROM AUTHOR]

Trace elements are micronutrient components that are only required in small amounts but are critical for the biological functions of many human body tissues. Studies in multiple settings found significant connections between diabetes mellitus (DM) and trace elements caused by disturbances of overlapping cellular metabolic systems. Zinc (Zn), boron (Br), chromium (Cr), and selenium (Se) at either extremely high or low levels could elicit some alteration in cellular metabolism. These lead to the development of DM. The changes include 1) the disturbance in the efficient release of insulin secretory granules, 2) the production of proinflammatory cytokines and oxidative stress state, 3) the failure of the insulin-signaling pathway, and 4) the reduction of glucose tissue uptake secondary to the downregulation of glucose-transporters. Both significantly high and low concentrations have been linked to the development of insulin resistance. Nevertheless, conflicting evidence makes their optimum nutritional levels difficult to establish. The purpose of this review is to emphasize the metabolic role of the 4 trace elements and their influence on the pathogenesis of DM when body levels are below optimal. Understanding the roles of these elements could pave the way for therapeutic possibilities and breakthroughs in personalized DM management. [ABSTRACT FROM AUTHOR]

Objective: The aim of the study was to determine the heavy metal and trace element (HMTE) profile in patients with migraine (PwM) and to compare it to that of healthy individuals without migraine. Background: Migraine is a universal disease that affects more than 10% of the world's population; however, its pathophysiology is still obscure. Methods: A total of 100 participants were included in this prospective matched case–control study (50 PwM during acute attack and 50 age‐ and sex‐matched healthy controls). The study was conducted in the university hospital in Yozgat, Turkey, where the inductively coupled plasma mass spectrometry system was used to measure the HMTE profile. The calibration curve was created with 11 points for heavy metals (arsenic [As], cadmium [Cd], cobalt [Co], lead [Pb], mercury [Hg], nickel [Ni], and tin [Sn]) and trace elements (antimony [Sb], chromium [Cr], copper [Cu], iron [Fe], magnesium [Mg], manganese [Mn], molybdenum [Mo], and zinc [Zn]). Results: The median age was 27 (23–37) years, and the female/male ratio was 37/13 for both groups. The PwM group had significantly higher As, Co, Pb, and Ni levels among the heavy metals (p = 0.033, 0.017, 0.022, and 0.021, respectively). Also, PwM had significantly lower Cr, Mg, and Zn levels among the trace elements (p = 0.007, 0.024, and < 0.001, respectively). The only trace element that was elevated in the PwM group was Mn (p = 0.001). The PwM and control groups did not differ in terms of Cd, Sn, Sb, Cu, Fe, and Mo (p = 0.165, 0.997, 0.195, 0.408, 0.440, and 0.252, respectively). Conclusion: Some HMTE parameters are altered in PwM, which may provide additional insight into understanding migraine etiology. Plain Language Summary: Changes in blood levels of heavy metal and trace elements (HMTEs) may contribute to migraine. We compared several different HMTEs in healthy people and patients with migraine (PwM) during an attack and found many differences in HMTEs between healthy people and PwM. We concluded that shifts in blood levels of HMTEs may play a role in migraine, and further research on this topic is needed. [ABSTRACT FROM AUTHOR]

Cadmium (Cd) contamination of farmland soils leads to Cd accumulation in crops and reduced micronutrient uptake, posing grave risks to food safety. Herein, we investigated the enrichment and transportation patterns of Cd and trace elements in different parts of six wheat genotypes grown in weakly alkaline Cd-contaminated soils via pot experiments. The results revealed that the wheat grain variety with high Cd accumulation (Ningmai13) demonstrated a 1.94-fold increase compared to the variety with low accumulation (Yanong0428). The transfer factor of Cd from wheat straw to grain ranged from 0.319 to 0.761, while the transfer factor of Cd from root to straw ranged from 0.167 to 0.461. Furthermore, the concentrations of other metals in wheat grains followed the order of Zn > Mn > Fe > Cu. There was a significant positive correlation between Cd and Mn in grains, indicating a potential synergistic effect. Overall, this study provides valuable insights into the regulation of micronutrient intake to modulate Cd uptake in wheat. [ABSTRACT FROM AUTHOR]

Significant and readily accessible orogenic gold deposits have been previously recognized, exploited, and progressively depleted. Innovative approaches are required to discover new and deeply buried deposits. Recently, trace element variations in apatite have been used to distinguish fertile and barren environments as reliable mineral exploration tools. In this study, machine learning models using Random Forest and Deep Neutral Network are utilized to assess the fertility of quartz veins and altered zones in the orogenic gold systems. The two models have been trained using trace element data of apatite, and the performance of both models yield good classification accuracy (∼90% on average) with low false positive rates. Feature importance analysis (Gini decrease and hidden layer weights) suggest that Pb, As, U, Sr, Eu, Mn, and Fe are the important parameters. Arsenic, U, Eu, Mn, and Fe are redox‐sensitive elements, with their concentrations responding to changes in fluid redox conditions. Strontium primarily originates from the breakdown of plagioclase, which is more likely to occur under oxidizing fluid conditions. Therefore, we infer that the main controlling factor of the models is the redox conditions. The two distinct models consistently highlight the most significant contribution of Pb to this differentiation, even though Pb is not a redox‐sensitive element and can only substitute for Ca2+ in apatite as Pb2+. We infer that the high contribution of Pb may be attributed to the potential transportation of Au in the form of a Pb‐(Bi)‐Au melt, and the Pb content in apatite is influenced by the Pb content in the melt, fluid oxygen, and sulfur fugacity. We also propose a novel discriminant plot using Linear Discriminant Analysis (LDA) to assess the mineralization potential in quartz veins and alteration zones based on apatite trace element data. The machine learning and LDA results suggest that apatite trace elements could be used effectively in the future orogenic gold deposit exploration. Key Points: Random Forests and Deep Neutral Network models demonstrate effective discrimination among various apatite types in orogenic gold depositsThe two distinct models consistently highlight the significant contribution of Pb to this differentiationA novel discriminant plot using Linear Discriminant Analysis (LDA) is proposed to assess the mineralization potential [ABSTRACT FROM AUTHOR]

The forest headwater streams are important hubs for connecting terrestrial and aquatic ecosystems, with plant litter and sediments as the major carriers for material migrations; however, until now we knew little about the dynamics of trace elements such as iron (Fe) and aluminum (Al) in forest headwater streams. Here, we quantitatively identified the spatiotemporal dynamics of Fe and Al storages in plant litter and sediments and their influencing factors in a subtropical forest headwater stream, and assessed the potential pollution risk. The results showed that: (1) the mean concentrations of Fe and Al in plant litter (sediments) were 5.48 and 8.46 (7.39 and 47.47) g·kg−1, and the mean storages of Fe and Al in plant litter (sediments) were 0.26 and 0.43 (749.04 and 5030.90) g·m−2, respectively; (2) the storages of Fe and Al in plant litter and sediments significantly fluctuated from January to December, and showed a decreasing pattern from the source to mouth; and (3) storages of Fe and Al had no significant correlation with riparian forest type and the present of tributary and the Fe and Al storages in plant litter were mainly affected by water temperature and water alkalinity, and their storages in sediments were mainly affected by water temperature and frequency of rainfall; and (4) there were no anthropogenic pollution in Fe and Al in the forest headwater stream. Our study revealed the primary factors of concentrations and storages of Fe and Al in plant litter and sediments in a forest headwater stream, which will improve our understanding of the role of headwater streams in forest nutrient storage and cycling along with hydrological processes. [ABSTRACT FROM AUTHOR]

The red deer is an ungulate and large game species. The contamination of the ecosystems by metal(loid)s may lead to the exposure of animals (as well as humans) through water and food resources. The direct contact of hunters and wild animal meat consumers with deer carcasses may be a potential contaminant source. This study aimed to determine the metal(loid)s’ concentrations in the liver and kidney of red deer from two regions of Portugal (Idanha-a-Nova and Lousã), and to relate these with histopathologic lesions. Thirteen young male deer were submitted to metal(loid) determination (As, Cd, Co, Cr, Cu, Pb, and Zn) by inductively coupled plasma mass spectrophotometry (ICP-MS) and histopathology examination. Renal Cd (8.072 ± 5.766 mg/kg dw) and hepatic Pb (3.824 ± 6.098 mg/kg dw) mean values were high, considering the maximum values for consumption established by the European Commission. The hepatic mean value of Cu was significantly higher in Idanha-a-Nova (150.059 ± 33.321 mg/kg dw), and it is at the Cu toxicity limit considered for ruminants (150 mg/kg). The pollution induced by Panasqueira mines (Castelo Branco) may be a possible explanation for some of the findings, especially the higher values of hepatic Cu and Pb found in Idanha-a-Nova deer. These results have high importance under a One Health perspective, since they have implications in public health, and pose at risk the imbalance of animal populations and ecosystems. [ABSTRACT FROM AUTHOR]

The deposition of metallic substances on farmland is of critical importance owing to its possible detrimental impacts on the surroundings and human wellness. Heavy metals can adversely affect the physicochemical properties of soil and plant health. Prolonged exposure to heavy metals in humans can lead to both carcinogenic and non-carcinogenic effects. The present research intended to determine the dispersion condition of toxic metals on cropland and assess the attendant environmental and health dangers in the Wonosobo Regency. This study used a soil sample survey encompassing 312 soil sampling sites. The completed analyses comprise geographical analysis, index of geo-accumulation (IG), contamination index (CI), Nemerow comprehensive contamination index (NCCI), risk analysis, and health risk analysis. The research findings reveal that cadmium material is the primary contributor to contamination from heavy metals in farmland in Wonosobo Regency, as shown by IG analysis. The CI and environmental hazards assessments suggest considerable pollution levels. Kids are more prone to illnesses than adults due to their daily intake amount, so it is necessary to pay more attention to the daily intake sources in children to minimize exposure to heavy metals. [ABSTRACT FROM AUTHOR]

[Display omitted] • A broad variety of radionuclides and elements were determined in SSA, APC and DFB. • Mobility of wide spectrum of elements in studied waste was examined. • Relatively high activity of 226Ra in SSA and DFB samples was observed. • The content of majority of elements in samples decrease in samples: SSA > DFB > APC. • High mobility of metalloids and low mobility of metals in SSA and DFB was observed. The research was aimed at providing new knowledge in the field of chemical characteristics of solid waste generated in the process of combustion of sewage sludge in fluidized bed furnaces. The research material consisted of disposed fluidized beds (DFB), sewage sludge ash (SSA) and air pollution control residues (APC) from three Polish installations for the thermal treatment of sewage sludge. Natural radionuclides as well as anthropogenic isotope 137Cs were determined in the tested materials and the migration of a wide spectrum of trace elements to various waste fractions generated in the process of sewage sludge combustion was examined. It was observed that both radioisotopes and most of the trace elements determined accumulate in SSA and DFB, while the APC fraction contains a much smaller amount of them. The exceptions are mercury and selenium, whose volatile compounds migrate to the exhaust gas dedusting system and accumulate in the APC fraction (up to 40 mg/kg and 13 mg/kg, respectively). A potential threat from the 226Ra isotope in SSA is identified in the context of the management of this waste in the production of building materials because the typical activity of 226Ra in SSA collected from areas with very low Ra content in natural environment exceeds 1.5–6 times the activity of this isotope in conventional cement mixtures. When managing SSA and DFB, special attention should be paid to the content of metalloids such as As, B and Se, due to the high content of mobile forms of these elements in the mentioned materials. [ABSTRACT FROM AUTHOR]

Green initiatives and phytotechnologies, such as phytoremediation, hold great potential for the sustainable management of contaminated brownfields. One impediment to these initiatives is the lack of alternatives for the produced biomass due to the potential contamination of plants by trace elements. We conducted an experiment to quantify the accumulation and the translocation patterns of trace elements in the aboveground parts of fifteen desirable plants species used for these kinds of initiatives, i.e. urban predominance, ecological relevance, edibility, commercial prospective, and phytoremediation potential. The experiment was set-up in a tunnel-type greenhouse in which plants were grown in pots filled with contaminated urban soil containing Cu, Zn, Se, Ag and spiked with Cd. Our results indicated that different species exhibited differential abilities to accumulate and translocate trace elements in their aboveground biomass and furthermore, exhibited different bioconcentration factors. A clear exclusion of Cd allocation was observed in fruits and flowers. However, Cd content in edible parts was still above the standard recommendations for human consumption. Our results suggest potential candidates for phytoremediation by revealing different accumulation patterns, also highlighting the importance of characterizing trace elements in the soil for selecting the appropriate plant species based on project's needs. [ABSTRACT FROM AUTHOR]

This study aimed to synthesize, characterize, and evaluate the effect of cocamidopropyl betaine-stabilized MnO2 nanoparticles (NPs) on the germination and development of pea seedlings. The synthesized NPs manifested as aggregates ranging from 50–600 nm, comprising spherical particles sized between 19 to 50 nm. These particles exhibited partial crystallization, indicated by peaks at 2θ = 25.37, 37.62, 41.18, 49.41, 61.45, and 65.79°, characteristic of MnO2 with a tetragonal crystal lattice with a I4/m spatial group. Quantum chemical modelling showed that the stabilization process of MnO2 NPs with cocamidopropyl betaine is energetically advantageous (∆E > 1299.000 kcal/mol) and chemically stable, as confirmed by the positive chemical hardness values (0.023 ≤ η ≤ 0.053 eV). It was revealed that the interaction between the MnO2 molecule and cocamidopropyl betaine, facilitated by a secondary amino group (NH), is the most probable scenario. This ascertain is supported by the values of the difference in total energy (∆E = 1299.519 kcal/mol) and chemical hardness (η = 0.053 eV). These findings were further confirmed using FTIR spectroscopy. The effect of MnO2 NPs at various concentrations on the germination of pea seeds was found to be nonlinear and ambiguous. The investigation revealed that MnO2 NPs at a concentration of 0.1 mg/L resulted in the highest germination energy (91.25%), germinability (95.60%), and lengths of roots and seedlings among all experimental samples. However, an increase in the concentration of preparation led to a slight growth suppression (1–10 mg/L) and the pronounced inhibition of seedling and root development (100 mg/L). The analysis of antioxidant indicators and phytochemicals in pea seedlings indicated that only 100 mg/L MnO2 NPs have a negative effect on the content of soluble sugars, chlorophyll a/b, carotenoids, and phenols. Conversely, lower concentrations showed a stimulating effect on photosynthesis indicators. Nevertheless, MnO2 NPs at all concentrations generally decreased the antioxidant potential of pea seedlings, except for the ABTS parameter. Pea seedlings showed a notable capacity to absorb Mn, reaching levels of 586.5 μg/L at 10 mg/L and 892.6 μg/L at 100 mg/L MnO2 NPs, surpassing the toxic level for peas according to scientific literature. However, the most important result was the observed growth-stimulating activity at 0.1 mg/L MnO2 NPs stabilized with cocamidopropyl betaine, suggesting a promising avenue for further research. [ABSTRACT FROM AUTHOR]