Search

Your search keyword '"Townsend, R. Reid"' showing total 509 results
Start Over Author "Townsend, R. Reid"
509 results on '"Townsend, R. Reid"'

3. Proteogenomic analysis reveals cytoplasmic sequestration of RUNX1 by the acute myeloid leukemia-initiating CBFB::MYH11 oncofusion protein

Author

Day, Ryan B., Hickman, Julia A., Xu, Ziheng, Katerndahl, Casey D.S., Ferraro, Francesca, Ramakrishnan, Sai Mukund, Erdmann-Gilmore, Petra, Sprung, Robert W., Mi, Yiling, Townsend, R. Reid, Miller, Christopher A., and Ley, Timothy J.

Subjects
Myosin -- Genetic aspects, Genes -- Genetic aspects, Biotin -- Genetic aspects, Muscle proteins -- Genetic aspects, B cells -- Genetic aspects, Ligases -- Genetic aspects, Health care industry
Abstract

Several canonical translocations produce oncofusion genes that can initiate acute myeloid leukemia (AML). Although each translocation is associated with unique features, the mechanisms responsible remain unclear. While proteins interacting with each oncofusion are known to be relevant for how they act, these interactions have not yet been systematically defined. To address this issue in an unbiased fashion, we fused a promiscuous biotin ligase (TurboID) in-frame with 3 favorablerisk AML oncofusion cDNAs (PML::RARA, RUNX1::RUNX1T1, and CBFB::MYH11) and identified their interacting proteins in primary murine hematopoietic cells. The PML::RARA- and RUNX1::RUNX1T1-TurboID fusion proteins labeled common and unique nuclear repressor complexes, implying their nuclear localization. However, CBFB::MYH11-TurboID-interacting proteins were largely cytoplasmic, probably because of an interaction of the MYH11 domain with several cytoplasmic myosin- related proteins. Using a variety of methods, we showed that the CBFB domain of CBFB::MYH11 sequesters RUNX1 in cytoplasmic aggregates; these findings were confirmed in primary human AML cells. Paradoxically, CBFB::MYH11 expression was associated with increased RUNX1/2 expression, suggesting the presence of a sensor for reduced functional RUNX1 protein, and a feedback loop that may attempt to compensate by increasing RUNX1/2 transcription. These findings may have broad implications for AML pathogenesis., Introduction Oncofusion genes exist in a variety of solid tumors and hematopoietic malignancies, and are often predictive of outcomes. Elucidating the mechanism of action of an oncofusion protein presents unique [...]

Published
2024
Full Text
View/download PDF

7. Proteomic and phosphoproteomic landscapes of acute myeloid leukemia

Author

Kramer, Michael H., Zhang, Qiang, Sprung, Robert, Day, Ryan B., Erdmann-Gilmore, Petra, Li, Yang, Xu, Ziheng, Helton, Nichole M., George, Daniel R., Mi, Yiling, Westervelt, Peter, Payton, Jacqueline E., Ramakrishnan, Sai M., Miller, Christopher A., Link, Daniel C., DiPersio, John F., Walter, Matthew J., Townsend, R. Reid, and Ley, Timothy J.

Published
2022
Full Text
View/download PDF

8. Missense mutations in Myc Box I influence nucleocytoplasmic transport to promote leukemogenesis.

Author

Arthur, Nancy BJ., primary, Christensen, Keegan A., additional, Mannino, Kathleen, additional, Ruzinova, Marianna B., additional, Kumar, Ashutosh, additional, Gruszczynska, Agata, additional, Day, Ryan B., additional, Erdmann-Gilmore, Petra, additional, Mi, Yiling, additional, Sprung, Robert, additional, York, Conner R., additional, Townsend, R. Reid., additional, Spencer, David H., additional, Sykes, Stephen M., additional, and Ferraro, Francesca, additional

Published
2024
Full Text
View/download PDF

9. Immune activation during Paenibacillus brain infection in African infants with frequent cytomegalovirus co-infection

Author

Isaacs, Albert M., Morton, Sarah U., Movassagh, Mercedeh, Zhang, Qiang, Hehnly, Christine, Zhang, Lijun, Morales, Diego M., Sinnar, Shamim A., Ericson, Jessica E., Mbabazi-Kabachelor, Edith, Ssenyonga, Peter, Onen, Justin, Mulondo, Ronnie, Hornig, Mady, Warf, Benjamin C., Broach, James R., Townsend, R. Reid, Limbrick, David D., Jr., Paulson, Joseph N., and Schiff, Steven J.

Published
2021
Full Text
View/download PDF

10. C-terminal phosphorylation of NaV1.5 impairs FGF13-dependent regulation of channel inactivation

Author

Burel, Sophie, Coyan, Fabien C, Lorenzini, Maxime, Meyer, Matthew R, Lichti, Cheryl F, Brown, Joan H, Loussouarn, Gildas, Charpentier, Flavien, Nerbonne, Jeanne M, Townsend, R Reid, Maier, Lars S, and Marionneau, Céline

Subjects
Heart Disease, Cardiovascular, Amino Acid Substitution, Animals, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Fibroblast Growth Factors, HEK293 Cells, Heart Failure, Humans, Ion Channel Gating, Mice, Mice, Transgenic, Mutation, Missense, NAV1.5 Voltage-Gated Sodium Channel, Phosphorylation, Ca2+/calmodulin-dependent protein kinase II, FGF13, Nav1.5, calmodulin, channel inactivation, heart, phosphoproteomics, phosphorylation, sodium channel, Chemical Sciences, Biological Sciences, Medical and Health Sciences, Biochemistry & Molecular Biology
Abstract

Voltage-gated Na+ (NaV) channels are key regulators of myocardial excitability, and Ca2+/calmodulin-dependent protein kinase II (CaMKII)-dependent alterations in NaV1.5 channel inactivation are emerging as a critical determinant of arrhythmias in heart failure. However, the global native phosphorylation pattern of NaV1.5 subunits associated with these arrhythmogenic disorders and the associated channel regulatory defects remain unknown. Here, we undertook phosphoproteomic analyses to identify and quantify in situ the phosphorylation sites in the NaV1.5 proteins purified from adult WT and failing CaMKIIδc-overexpressing (CaMKIIδc-Tg) mouse ventricles. Of 19 native NaV1.5 phosphorylation sites identified, two C-terminal phosphoserines at positions 1938 and 1989 showed increased phosphorylation in the CaMKIIδc-Tg compared with the WT ventricles. We then tested the hypothesis that phosphorylation at these two sites impairs fibroblast growth factor 13 (FGF13)-dependent regulation of NaV1.5 channel inactivation. Whole-cell voltage-clamp analyses in HEK293 cells demonstrated that FGF13 increases NaV1.5 channel availability and decreases late Na+ current, two effects that were abrogated with NaV1.5 mutants mimicking phosphorylation at both sites. Additional co-immunoprecipitation experiments revealed that FGF13 potentiates the binding of calmodulin to NaV1.5 and that phosphomimetic mutations at both sites decrease the interaction of FGF13 and, consequently, of calmodulin with NaV1.5. Together, we have identified two novel native phosphorylation sites in the C terminus of NaV1.5 that impair FGF13-dependent regulation of channel inactivation and may contribute to CaMKIIδc-dependent arrhythmogenic disorders in failing hearts.

Published
2017

11. HTLV-1 infected T cells cause bone loss via small extracellular vesicles

Author

Pokhrel, Nitin Kumar, primary, Panfil, Amanda, additional, Habib, Haniya, additional, Seeniraj, Sham, additional, Joseph, Ancy, additional, Rauch, Daniel, additional, Cox, Linda, additional, Sprung, Robert, additional, Gilmore, Petra Erdmann, additional, Zhang, Qiang, additional, Townsend, R Reid, additional, Yu, Lianbo, additional, Yilmaz, Ayse Selen, additional, Aurora, Rajeev, additional, Park, William, additional, Ratner, Lee, additional, Weilbaecher, Katherine N, additional, and Veis, Deborah J, additional

Published
2024
Full Text
View/download PDF

12. Targeted peptide measurements in biology and medicine: best practices for mass spectrometry-based assay development using a fit-for-purpose approach.

Author

Carr, Steven A, Abbatiello, Susan E, Ackermann, Bradley L, Borchers, Christoph, Domon, Bruno, Deutsch, Eric W, Grant, Russell P, Hoofnagle, Andrew N, Hüttenhain, Ruth, Koomen, John M, Liebler, Daniel C, Liu, Tao, MacLean, Brendan, Mani, DR, Mansfield, Elizabeth, Neubert, Hendrik, Paulovich, Amanda G, Reiter, Lukas, Vitek, Olga, Aebersold, Ruedi, Anderson, Leigh, Bethem, Robert, Blonder, Josip, Boja, Emily, Botelho, Julianne, Boyne, Michael, Bradshaw, Ralph A, Burlingame, Alma L, Chan, Daniel, Keshishian, Hasmik, Kuhn, Eric, Kinsinger, Christopher, Lee, Jerry SH, Lee, Sang-Won, Moritz, Robert, Oses-Prieto, Juan, Rifai, Nader, Ritchie, James, Rodriguez, Henry, Srinivas, Pothur R, Townsend, R Reid, Van Eyk, Jennifer, Whiteley, Gordon, Wiita, Arun, and Weintraub, Susan

Subjects
Animals, Humans, Peptides, Biological Assay, Isotope Labeling, Proteomics, Biology, Medicine, Reference Standards, Software, Mass Spectrometry, Guidelines as Topic, Biochemistry & Molecular Biology
Abstract

Adoption of targeted mass spectrometry (MS) approaches such as multiple reaction monitoring (MRM) to study biological and biomedical questions is well underway in the proteomics community. Successful application depends on the ability to generate reliable assays that uniquely and confidently identify target peptides in a sample. Unfortunately, there is a wide range of criteria being applied to say that an assay has been successfully developed. There is no consensus on what criteria are acceptable and little understanding of the impact of variable criteria on the quality of the results generated. Publications describing targeted MS assays for peptides frequently do not contain sufficient information for readers to establish confidence that the tests work as intended or to be able to apply the tests described in their own labs. Guidance must be developed so that targeted MS assays with established performance can be made widely distributed and applied by many labs worldwide. To begin to address the problems and their solutions, a workshop was held at the National Institutes of Health with representatives from the multiple communities developing and employing targeted MS assays. Participants discussed the analytical goals of their experiments and the experimental evidence needed to establish that the assays they develop work as intended and are achieving the required levels of performance. Using this "fit-for-purpose" approach, the group defined three tiers of assays distinguished by their performance and extent of analytical characterization. Computational and statistical tools useful for the analysis of targeted MS results were described. Participants also detailed the information that authors need to provide in their manuscripts to enable reviewers and readers to clearly understand what procedures were performed and to evaluate the reliability of the peptide or protein quantification measurements reported. This paper presents a summary of the meeting and recommendations.

Published
2014

13. Proteogenomic analysis reveals cytoplasmic sequestration of RUNX1 by the acute myeloid leukemia–initiating CBFB::MYH11 oncofusion protein

Author

Day, Ryan B., primary, Hickman, Julia A., additional, Xu, Ziheng, additional, Katerndahl, Casey D.S., additional, Ferraro, Francesca, additional, Ramakrishnan, Sai Mukund, additional, Erdmann-Gilmore, Petra, additional, Sprung, Robert W., additional, Mi, Yiling, additional, Townsend, R. Reid, additional, Miller, Christopher A., additional, and Ley, Timothy J., additional

Published
2023
Full Text
View/download PDF

14. Human Experimental Challenge With Enterotoxigenic Escherichia coli Elicits Immune Responses to Canonical and Novel Antigens Relevant to Vaccine Development

Author

Chakraborty, Subhra, Randall, Arlo, Vickers, Tim J., Molina, Doug, Harro, Clayton D., DeNearing, Barbara, Brubaker, Jessica, Sack, David A., Bourgeois, A. Louis, Felgner, Philip. L., Liang, Xiaowu, Mani, Sachin, Wenzel, Heather, Townsend, R. Reid, Gilmore, Petra E., Darsley, Michael J., Rasko, David A., and Fleckenstein, James M.

Published
2018

20. Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography–mass spectrometry

Author

Mertins, Philipp, Tang, Lauren C., Krug, Karsten, Clark, David J., Gritsenko, Marina A., Chen, Lijun, Clauser, Karl R., Clauss, Therese R., Shah, Punit, Gillette, Michael A., Petyuk, Vladislav A., Thomas, Stefani N., Mani, D. R., Mundt, Filip, Moore, Ronald J., Hu, Yingwei, Zhao, Rui, Schnaubelt, Michael, Keshishian, Hasmik, Monroe, Matthew E., Zhang, Zhen, Udeshi, Namrata D., Mani, Deepak, Davies, Sherri R., Townsend, R. Reid, Chan, Daniel W., Smith, Richard D., Zhang, Hui, Liu, Tao, and Carr, Steven A.

Published
2018
Full Text
View/download PDF

21. Determinants of iFGF13-mediated regulation of myocardial voltage-gated sodium (NaV) channels in mouse

Author

Lesage, Adrien, Lorenzini, Maxime, Burel, Sophie, Sarlandie, Marine, Bibault, Floriane, Lindskog, Cecilia, Maloney, Daniel, Silva, Jonathan R., Townsend, R. Reid, Nerbonne, Jeanne M., Marionneau, Celine, Lesage, Adrien, Lorenzini, Maxime, Burel, Sophie, Sarlandie, Marine, Bibault, Floriane, Lindskog, Cecilia, Maloney, Daniel, Silva, Jonathan R., Townsend, R. Reid, Nerbonne, Jeanne M., and Marionneau, Celine

Abstract

Posttranslational regulation of cardiac Na(V)1.5 channels is critical in modulating channel expression and function, yet their regulation by phosphorylation of accessory proteins has gone largely unexplored. Using phosphoproteomic analysis of Na-V channel complexes from adult mouse left ventricles, we identified nine phosphorylation sites on intracellular fibroblast growth factor 13 (iFGF13). To explore the potential roles of these phosphosites in regulating cardiac NaV currents, we abolished expression of iFGF13 in neonatal and adult mouse ventricular myocytes and rescued it with wild-type (WT), phosphosilent, or phosphomimetic iFGF13-VY. While the increased rate of closed-state inactivation of NaV channels induced by Fgf13 knockout in adult cardiomyocytes was completely restored by adenoviral-mediated expression of WT iFGF13-VY, only partial rescue was observed in neonatal cardiomyocytes after knockdown. The knockdown of iFGF13 in neonatal ventricular myocytes also shifted the voltage dependence of channel activation toward hyperpolarized potentials, a shift that was not reversed by WT iFGF13-VY expression. Additionally, we found that iFGF13-VY is the predominant isoform in adult ventricular myocytes, whereas both iFGF13-VY and iFGF13-S are expressed comparably in neonatal ventricular myocytes. Similar to WT iFGF13-VY, each of the iFGF13-VY phosphomutants studied restored NaV channel inactivation properties in both models. Lastly, Fgf13 knockout also increased the late Na+ current in adult cardiomyocytes, and this effect was restored with expression of WT and phosphosilent iFGF13-VY. Together, our results demonstrate that iFGF13 is highly phosphorylated and displays differential isoform expression in neonatal and adult ventricular myocytes. While we found no roles for iFGF13 phosphorylation, our results demonstrate differential effects of iFGF13 on neonatal and adult mouse ventricular NaV channels.

Published
2023
Full Text
View/download PDF

22. Interrogation of a live-attenuated enterotoxigenic Escherichia coli vaccine highlights features unique to wild-type infection

Author

Chakraborty, Subhra, Randall, Arlo, Vickers, Tim J., Molina, Doug, Harro, Clayton D., DeNearing, Barbara, Brubaker, Jessica, Sack, David A., Bourgeois, A. Louis, Felgner, Philip L., Liang, Xiaowu, Mani, Sachin, Wenzel, Heather, Townsend, R. Reid, Gilmore, Petra E., Darsley, Michael J., Rasko, David A., and Fleckenstein, James M.

Published
2019
Full Text
View/download PDF

23. Supplementary File 5 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
Full Text
View/download PDF

24. Supplementary File 1 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
Full Text
View/download PDF

25. Supplementary File 2 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
Full Text
View/download PDF

26. Extended Methods from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
Full Text
View/download PDF

27. Supplementary File 3 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
Full Text
View/download PDF

28. Supplementary Figures from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
Full Text
View/download PDF

29. Supplementary tables 1 and 2 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
Full Text
View/download PDF

30. Data from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
Full Text
View/download PDF

31. Supplementary File 4 from Mass Spectrometry–Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers

Author

Mundt, Filip, primary, Rajput, Sandeep, primary, Li, Shunqiang, primary, Ruggles, Kelly V., primary, Mooradian, Arshag D., primary, Mertins, Philipp, primary, Gillette, Michael A., primary, Krug, Karsten, primary, Guo, Zhanfang, primary, Hoog, Jeremy, primary, Erdmann-Gilmore, Petra, primary, Primeau, Tina, primary, Huang, Shixia, primary, Edwards, Dean P., primary, Wang, Xiaowei, primary, Wang, Xuya, primary, Kawaler, Emily, primary, Mani, D.R., primary, Clauser, Karl R., primary, Gao, Feng, primary, Luo, Jingqin, primary, Davies, Sherri R., primary, Johnson, Gary L., primary, Huang, Kuan-lin, primary, Yoon, Christopher J., primary, Ding, Li, primary, Fenyö, David, primary, Ellis, Matthew J., primary, Townsend, R. Reid, primary, Held, Jason M., primary, Carr, Steven A., primary, and Ma, Cynthia X., primary

Published
2023
Full Text
View/download PDF

38. Targeted proteomic quantitation of NRF2 signaling and predictive biomarkers in HNSCC

Author

Wamsley, Nathan, primary, Wilkerson, Emily, additional, Guan, Li, additional, LaPak, Kyle, additional, Schrank, Travis, additional, Holmes, Brittany, additional, Sprung, Robert, additional, Gilmore, Petra Erdmann, additional, Gerndt, Sophie, additional, Jackson, Ryan, additional, Paniello, Randal, additional, Pipkorn, Patrik, additional, Puram, Sidarth V, additional, Rich, Jason, additional, Townsend, R Reid, additional, Zevallos, Jose, additional, Zolkind, Paul, additional, Le, Quynh-Thu, additional, Goldfarb, Dennis, additional, and Major, Michael B, additional

Published
2023
Full Text
View/download PDF

39. Excretory/Secretory Proteome of Females and Males of the Hookworm Ancylostoma ceylanicum

Author

Uzoechi, Samuel C., primary, Rosa, Bruce A., additional, Singh, Kumar Sachin, additional, Choi, Young-Jun, additional, Bracken, Bethany K., additional, Brindley, Paul J., additional, Townsend, R. Reid, additional, Sprung, Robert, additional, Zhan, Bin, additional, Bottazzi, Maria-Elena, additional, Hawdon, John M., additional, Wong, Yide, additional, Loukas, Alex, additional, Djuranovic, Sergej, additional, and Mitreva, Makedonka, additional

Published
2023
Full Text
View/download PDF

40. sj-docx-1-ajs-10.1177_03635465231169526 – Supplemental material for Synovial Fluid Proteomics From Serial Aspirations of ACL-Injured Knees Identifies Candidate Biomarkers

Author

Rai, Muhammad Farooq, Cai, Lei, Zhang, Qiang, Townsend, R. Reid, and Brophy, Robert H.

Subjects
FOS: Clinical medicine, 110323 Surgery, 110604 Sports Medicine, FOS: Health sciences, 110314 Orthopaedics
Abstract

Supplemental material, sj-docx-1-ajs-10.1177_03635465231169526 for Synovial Fluid Proteomics From Serial Aspirations of ACL-Injured Knees Identifies Candidate Biomarkers by Muhammad Farooq Rai, Lei Cai, Qiang Zhang, R. Reid Townsend and Robert H. Brophy in The American Journal of Sports Medicine

Published
2023
Full Text
View/download PDF

41. Type IV Pili Are a Critical Virulence Factor in Clinical Isolates of Paenibacillus thiaminolyticus

Author

Hehnly, Christine, primary, Shi, Aiqin, additional, Ssentongo, Paddy, additional, Zhang, Lijun, additional, Isaacs, Albert, additional, Morton, Sarah U., additional, Streck, Nicholas, additional, Erdmann-Gilmore, Petra, additional, Tolstoy, Igor, additional, Townsend, R. Reid, additional, Limbrick, David D., additional, Paulson, Joseph N., additional, Ericson, Jessica E., additional, Galperin, Michael Y., additional, Schiff, Steven J., additional, and Broach, James R., additional

Published
2022
Full Text
View/download PDF

47. Synovial Fluid Proteomics From Serial Aspirations of ACL-Injured Knees Identifies Candidate Biomarkers.

Author

Rai, Muhammad Farooq, Cai, Lei, Zhang, Qiang, Townsend, R. Reid, and Brophy, Robert H.

Subjects
PROTEIN analysis, KNEE osteoarthritis, BIOMARKERS, HOMEOSTASIS, RESEARCH methodology, LIQUID chromatography, RISK assessment, PROTEOMICS, ANTERIOR cruciate ligament injuries, MASS spectrometry, RESEARCH funding, SYNOVIAL fluid, DISEASE risk factors, DISEASE complications
Abstract

Background: Anterior cruciate ligament (ACL) tears often result in knee effusion and an increased risk for developing knee osteoarthritis (OA) in the long run. The molecular profile of these effusions could be informative regarding initial steps in the development of posttraumatic OA after an ACL tear. Hypothesis: The proteomics of knee synovial fluid changes over time after ACL injury. Study Design: Descriptive laboratory study. Methods: Synovial fluid was collected from patients with an acute traumatic ACL tear presenting to the office for evaluation (18.31 ± 19.07 days from injury) (aspiration 1) and again at the time of surgery (35.41 ± 58.15 days after aspiration 1 (aspiration 2). High-resolution liquid chromatography mass spectrometry was used to assess the quantitative protein profile of synovial fluid, and differences in protein profile between the 2 aspirations were determined computationally. Results: A total of 58 synovial fluid samples collected from 29 patients (12 male, 17 female; 12 isolated ACL tear, 17 combined ACL and meniscal tear) with a mean age and body mass index of 27.01 ± 12.78 years and 26.30 ± 4.93, respectively, underwent unbiased proteomics analysis. The levels of 130 proteins in the synovial fluid changed over time (87 high, 43 low). Proteins of interest that were significantly higher in aspiration 2 included CRIP1, S100A11, PLS3, POSTN, and VIM, which represent catabolic/inflammatory activities in the joint. Proteins with a known role in chondroprotection and joint homeostasis such as CHI3L2 (YKL-39), TNFAIP6/TSG6, DEFA1, SPP1, and CILP were lower in aspiration 2. Conclusion: Synovial fluid from knees with ACL tears exhibits an increased burden of inflammatory (catabolic) proteins relevant to OA with reduced levels of chondroprotective (anabolic) proteins. Clinical Relevance: This study identified a set of novel proteins that provide new biological insights into the aftermath of ACL tears. Elevated inflammation and decreased chondroprotection could represent initial disruption of homeostasis, potentially initiating the development of OA. Longitudinal follow-up and mechanistic studies are necessary to assess the functional role of these proteins in the joint. Ultimately, these investigations could lead to better approaches to predict and possibly improve patient outcomes. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

48. Using the CPTAC Assay Portal to Identify and Implement Highly Characterized Targeted Proteomics Assays

Author

Whiteaker, Jeffrey R., primary, Halusa, Goran N., additional, Hoofnagle, Andrew N., additional, Sharma, Vagisha, additional, MacLean, Brendan, additional, Yan, Ping, additional, Wrobel, John A., additional, Kennedy, Jacob, additional, Mani, D. R., additional, Zimmerman, Lisa J., additional, Meyer, Matthew R., additional, Mesri, Mehdi, additional, Boja, Emily, additional, Carr, Steven A., additional, Chan, Daniel W., additional, Chen, Xian, additional, Chen, Jing, additional, Davies, Sherri R., additional, Ellis, Matthew J. C., additional, Fenyö, David, additional, Hiltke, Tara, additional, Ketchum, Karen A., additional, Kinsinger, Chris, additional, Kuhn, Eric, additional, Liebler, Daniel C., additional, Liu, Tao, additional, Loss, Michael, additional, MacCoss, Michael J., additional, Qian, Wei-Jun, additional, Rivers, Robert, additional, Rodland, Karin D., additional, Ruggles, Kelly V., additional, Scott, Mitchell G., additional, Smith, Richard D., additional, Thomas, Stefani, additional, Townsend, R. Reid, additional, Whiteley, Gordon, additional, Wu, Chaochao, additional, Zhang, Hui, additional, Zhang, Zhen, additional, Rodriguez, Henry, additional, and Paulovich, Amanda G., additional

Published
2016
Full Text
View/download PDF

49. sj-pdf-1-ajs-10.1177_03635465221112652 – Supplemental material for Proteomic Profile Analysis of Synovial Fluid in Patients With Anterior Cruciate Ligament Tears

Author

Brophy, Robert H., Cai, Lei, Zhang, Qiang, Townsend, R. Reid, and Rai, Muhammad Farooq

Subjects
FOS: Clinical medicine, 110323 Surgery, 110604 Sports Medicine, FOS: Health sciences, 110314 Orthopaedics
Abstract

Supplemental material, sj-pdf-1-ajs-10.1177_03635465221112652 for Proteomic Profile Analysis of Synovial Fluid in Patients With Anterior Cruciate Ligament Tears by Robert H. Brophy, Lei Cai, Qiang Zhang, R. Reid Townsend and Muhammad Farooq Rai in The American Journal of Sports Medicine

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results