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436 results on '"Totet A"'

2. Nosocomial Pneumocystis jirovecii infections

Author

Nevez G., Chabé M., Rabodonirina M., Virmaux M., Dei-Cas E., Hauser P.M., and Totet A.

Subjects
Pneumocystis jirovecii, nosocomial infections, genotyping, Infectious and parasitic diseases, RC109-216
Abstract

Airborne transmission of Pneumocystis sp. from host to host has been demonstrated in rodent models and several observations suggest that interindividual transmission occurs in humans. Moreover, it is accepted that the Pneumocystis organisms infecting each mammalian species are host specific and that the hypothesis of an animal reservoir for Pneumocystis jirovecii (P. jirovecii), the human-specific Pneumocystis species, can be excluded. An exosaprophytic form of the fungus cannot be strictly ruled out. However, these data point toward the potential for the specific host to serve as its own reservoir and for Pneumocystis infection in humans as an anthroponosis with humans as a reservoir for P. jirovecii. This review highlights the main data on host-to-host transmission of Pneumocystis in rodent models and in humans by the airborne route and provides a rationale for considering the occurrence of nosocomial infections and measures for their prevention

Published
2008
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3. Pulmonary co-infections by Pneumocystis jirovecii and Herpesviridae: a seven-year retrospective study

Author

Alan Rucar, Anne Totet, Yohann Le Govic, Baptiste Demey, and Céline Damiani

Subjects
Pneumocystis jirovecii, Pneumocystis pneumonia, Pneumocystis pulmonary colonization, Pulmonary coinfections, Herpesviridae, Therapeutics. Pharmacology, RM1-950, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502
Abstract

Abstract Background Pneumocystis jirovecii (P. jirovecii) is an opportunistic fungus responsible for Pneumocystis pneumonia (PCP) in deeply immunocompromised patients and for pulmonary colonization in individuals with mild immunosuppression or impaired respiratory function. PCP and Cytomegalovirus (CMV) co-infections have been widely described whereas those involving other Herpesviruses (HVs) such as Epstein-Barr virus (EBV), Herpes simplex virus type 1 and type 2 (HSV-1 and -2), and Varicella zoster virus (VZV) remain scarce. To date, no data are available concerning HVs co-infections in P. jirovecii colonization. Methods Our main objective was to evaluate the frequency of HVs in bronchoalveolar lavage fluid (BALF) samples from patients with PCP or with pulmonary colonization. The secondary objective was to assess the relationship between HVs and the mortality rate in PCP patients. A retrospective single-center study over a seven-year period was conducted. All patients with P. jirovecii detected using PCR in a BALF sample and for whom a PCR assay for HVs detection was performed were included in the study. Results One hundred and twenty-five patients were included, corresponding to 77 patients with PCP and 48 colonized patients. At least one HV was detected in 54/77 (70.1%) PCP patients and in 28/48 (58.3%) colonized patients. EBV was the most frequent in both groups. Furthermore, the 30-day survival rate in PCP patients was significantly lower with [EBV + CMV] co-infection than that with EBV co-infection, [EBV + HSV-1] co-infection and without HV co-infection. Conclusion Our results show that the frequency of HV, alone or in combination is similar in PCP and colonization. They also suggest that [EBV + CMV] detection in BALF samples from PCP patients is associated with an increased mortality rate, underlying the significance to detect HVs in the course of PCP.

Published
2024
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7. Synthesis and Antiprotozoal Evaluation of New 2,9-bis[(pyridinylalkylaminomethyl) phenyl]-1,10-Phenanthroline Derivatives by Targeting G-quadruplex, an Interesting Pharmacophore Against Drug Efflux

Author

Jean Guillon, Anita Cohen, Sarah Monic, Clotilde Boudot, Solène Savrimoutou, Sandra Albenque-Rubio, Stéphane Moreau, Alexandra Dassonville-Klimpt, Jean-Louis Mergny, Luisa Ronga, Mikel Bernabeu de Maria, Nikita Tyurin-Schmitt, Paul Parrens, Adrien Labarthe, Valentin Gomez, Serge Moukha, Pascale Dozolme, Nadine Azas, Valérie Gabelica, Patrice Agnamey, Céline Damiani, Anne Totet, Catherine Mullié, Bertrand Courtioux, and Pascal Sonnet

Subjects
General Materials Science
Published
2023

9. Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study

Author

Alexandre Alanio, Maud Gits-Muselli, Nicolas Guigue, Marie Desnos-Ollivier, Enrique J. Calderon, David Di Cave, Damien Dupont, Axel Hamprecht, Philippe M. Hauser, Jannik Helweg-Larsen, Marta Kicia, Katrien Lagrou, Martina Lengerova, Olga Matos, Willem J.G. Melchers, Florent Morio, Gilles Nevez, Anne Totet, Lewis P. White, and Stéphane Bretagne

Subjects
Pneumocystis jirovecii, Genotyping, Europe, Transmission, Mixed infection, MLS typing, Microsatellites, Medicine, Medicine (General), R5-920
Abstract

Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting >500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotyping was performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11−20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., ≥2 alleles for ≥1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p

Published
2017
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10. Synthesis and Antiprotozoal Evaluation of New 2,9-bis[(pyridinylalkylaminomethyl) phenyl]-1,10-Phenanthroline Derivatives by Targeting G-quadruplex, an Interesting Pharmacophore Against Drug Efflux

Author

Guillon, Jean, primary, Cohen, Anita, additional, Monic, Sarah, additional, Boudot, Clotilde, additional, Savrimoutou, Solène, additional, Albenque-Rubio, Sandra, additional, Moreau, Stéphane, additional, Dassonville-Klimpt, Alexandra, additional, Mergny, Jean-Louis, additional, Ronga, Luisa, additional, de Maria, Mikel Bernabeu, additional, Tyurin-Schmitt, Nikita, additional, Parrens, Paul, additional, Labarthe, Adrien, additional, Gomez, Valentin, additional, Moukha, Serge, additional, Dozolme, Pascale, additional, Azas, Nadine, additional, Gabelica, Valérie, additional, Agnamey, Patrice, additional, Damiani, Céline, additional, Totet, Anne, additional, Mullié, Catherine, additional, Courtioux, Bertrand, additional, and Sonnet, Pascal, additional

Published
2023
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11. Active Surveillance Program to Increase Awareness on Invasive Fungal Diseases: the French RESSIF Network (2012 to 2018)

Author

Bretagne, Stéphane, Sitbon, Karine, Desnos-Ollivier, Marie, Garcia-Hermoso, Dea, Letscher-Bru, Valérie, Cassaing, Sophie, Millon, Laurence, Morio, Florent, Gangneux, Jean-Pierre, Hasseine, Lilia, Favennec, Loïc, Cateau, Estelle, Bailly, Eric, Moniot, Maxime, Bonhomme, Julie, Desbois-Nogard, Nicole, Chouaki, Taieb, Paugam, André, Bouteille, Bernard, Pihet, Marc, Dalle, Frédéric, Eloy, Odile, Sasso, Milène, Demar, Magalie, Mariani-Kurkdjian, Patricia, Robert, Vincent, Lortholary, Olivier, Dromer, Françoise, French Mycoses Study Group, The, Damiani, Céline, Dupont, Hervé, Maizel, Julien, Totet, Anne, Bouchara, Jean-Philippe, Bellanger, Anne Pauline, Berceanu, Ana, Navellou, Jean Christophe, Scherer, Emeline, Abboud, Philippe, Aznar, Christine, Blanchet, Denis, Djossou, Félix, Cayot, Sophie, Garrouste, Cyril, Lesens, Olivier, Moluçon, Cécile, Nourrisson, Céline, Poirier, Philippe, Bailly, Éloïse, Basmaciyan, Louise, Belaid, Bouhemad, Blot, Mathieu, Caillot, Denis, Charles, Pierre-Emmanuel, Quenot, Jean Pierre, Amazan, Emmanuelle, Baubion, Emilie, Cabie, André, Chabartier, Cyrille, Deligny, Christophe, Emal, Violaine, Flechelle, Olivier, Hatchuel, Yves, Le Govic, Yohann, Merle, Harold, Miossec, Charline, Turmel, Jean-Marie, Valentino, Ruddy, Durieux, Marie-Fleur, Turlure, Pascal, Mercier, Victor, Gari-Toussaint, Martine, Risso, Karine, Simon, Loïc, Bretonnière, Cedric, Boutoille, David, Gastinne, Thomas, Lakhal, Karim, Launay, Elise, Lepoivre, Thierry, Peterlin, Pierre, Rialland, Fanny, Le Pape, Patrice, Canoui, Etienne, Dahane, Naima, Kerneis, Solène, Angebault, Cécile, Bougnoux, Marie-Elisabethh, Guennouni, Nadia, Lanternier, Fanny, Neven, Bennedicte, Oualha, Mehdi, Scemla, Anne, Sitterlé, Emilie, Suarez, Felipe, Toubiana, Julie, Bonacorci, Stéphane, Alanio, Alexandre, Bergeron, Anne, Denis, Blandine, Gits-Muselli, Maud, Hamane, Samia, Touratier, Sophie, Chaumeil, Christine, Merabet, Lilia, Claudéon, Joelle, Curlier, Elodie, Galantine, Valérie, Gallois, Jean Claude, Markowicz, Samuel, Nicolas, Muriel, Musson, Pascal, Schepers, Kinda, Minoza, Alida, Kauffmann-Lacroix, Catherine, Guégan, Hélène, Costa, Damien, Dehais, Marion, Gargala, Gilles, Bajolet, Odile, Banisadr, Firouze, Cousson, Joel, Himberlin, Chantal, Huguenin, Antoine, Toubas, Dominique, Flori, Pierre, Mahinc, Caroline, Raberin, Hélène, Denis, Julie, Herbrecht, Raoul, Mertes, Paul-Michel, Meziani, Fehrat, Sabou, Marcela, Schneider, Francis, Bertozzi, Anne-Isabelle, Chauvin, Pamela, Charpentier, Elena, Cointault, Olivier, Cottrel, Claire, Delavigne, Karen, Faguer, Stanislas, Fillaux, Judith, Guemas, Emilie, Iriart, Xavier, Lelièvre, Lucie, Ruiz, Jean, Bastides, Frédéric, Chesnay, Adélaïde, Desoubeaux, Guillaume, Therby, Audrey, Chachaty, Elisabeth, Gachot, Bertrand, Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Software and Databasing, Mycologie moléculaire - Molecular Mycology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals (CNRMA), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Dynamique des interactions hôte pathogène (DIHP), Université de Strasbourg (UNISTRA), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Laboratoire Chrono-environnement (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Nantes Université (Nantes Univ), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre Hospitalier Universitaire de Nice (CHU Nice), CHU Rouen, Normandie Université (NU), Centre hospitalier universitaire de Poitiers (CHU Poitiers), Ecologie et biologie des interactions (EBI), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), CHU Clermont-Ferrand, CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), CHU de la Martinique [Fort de France], CHU Amiens-Picardie, Hôpital Cochin [AP-HP], CHU Limoges, SFR UA 4208 Interactions Cellulaires et Applications Thérapeutiques (ICAT), Université d'Angers (UA), Laboratoire de Parasitologie-Mycologie (CHU d'Angers), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Infections Respiratoires Fongiques (IRF), Université d'Angers (UA)-Université de Brest (UBO), Procédés Alimentaires et Microbiologiques [Dijon] (PAM), Université de Bourgogne (UB)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre Hospitalier de Versailles André Mignot (CHV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Centre Hospitalier Andrée Rosemon [Cayenne, Guyane Française], Hôpital Robert Debré Paris, Hôpital Robert Debré, Westerdijk Fungal Biodiversity Institute [Utrecht] (WI), Royal Netherlands Academy of Arts and Sciences (KNAW), Service des Maladies infectieuses et tropicales [CHU Necker], CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Agents infectieux, résistance et chimiothérapie - UR UPJV 4294 (AGIR ), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Simplification des soins chez les patients complexes - UR UPJV 7518 (SSPC), Université de Picardie Jules Verne (UPJV), Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV), Laboratoire de Parasitologie-Mycologie [CHU Angers], Hôpital Bretonneau, Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by recurrent financial support from Santé Publique France and Institut Pasteur. The funders had no role in study design, data collection, analysis, interpretation of data, or the decision to submit the work for publication., We thank Didier Che (Direction des Maladies Infectieuses, Santé Publique France) for his continuous support and interest in invasive fungal infections and Etienne Sevin (EpiConcept, Paris, France) for his contribution in the development of the RESSIF website using the VOOZANOO platform. We are thankful for the technical help of Catherine Blanc, Anne Boullié, Cécile Gautier, Virginie Geolier, and Damien Hoinard (Institut Pasteur) for the characterization of the isolates received at the NRCMA., The French Mycoses Study Group includes collaborators who contributed to these data by their involvement in the management of patients, expertise in diagnostic tools, and/or characterization of fungal isolates. They are listed in alphabetical order of a city in France and for each center, in alphabetical order of the last names: in Amiens, Céline Damiani, Hervé Dupont, Julien Maizel, and Anne Totet, in Angers, Jean-Philippe Bouchara, in Besançon, Anne Pauline Bellanger, Ana Berceanu, Jean Christophe Navellou, and Emeline Scherer, in Cayenne, Philippe Abboud, Christine Aznar, Denis Blanchet, and Félix Djossou, in Clermont-Ferrand, Sophie Cayot, Cyril Garrouste, Olivier Lesens, Cécile Moluçon, Céline Nourrisson, and Philippe Poirier, in Dijon, Éloïse Bailly, Louise Basmaciyan, Bouhemad Belaid, Mathieu Blot, Denis Caillot, Pierre-Emmanuel Charles, and Jean Pierre Quenot, in Fort de France, Emmanuelle Amazan, Emilie Baubion, André Cabie, Cyrille Chabartier, Christophe Deligny, Violaine Emal, Olivier Flechelle, Yves Hatchuel, Yohann Le Govic, Harold Merle, Charline Miossec, Jean-Marie Turmel, and Ruddy Valentino, in Limoges, Marie-Fleur Durieux and Pascal Turlure, in Nîmes, Victor Mercier, in Nice, Martine Gari-Toussaint, Karine Risso, and Loïc Simon, in Nantes, Cedric Bretonnière, David Boutoille, Thomas Gastinne, Karim Lakhal, Elise Launay, Thierry Lepoivre, Pierre Peterlin, Fanny Rialland, and Patrice Le Pape, in Paris, in Hôpital Cochin, Etienne Canoui, Naima Dahane, and Solène Kerneis, in Hôpital Necker-Enfants Malades, Cécile Angebault, Marie-Elisabethh Bougnoux, Nadia Guennouni, Fanny Lanternier, Bennedicte Neven, Mehdi Oualha, Anne Scemla, Emilie Sitterlé, Felipe Suarez, and Julie Toubiana, in Hôpital Robert-Debré, Stéphane Bonacorci, in Hôpital Saint-Louis, Alexandre Alanio, Anne Bergeron, Blandine Denis, Maud Gits-Muselli, Samia Hamane, and Sophie Touratier, in hôpital des XV-XX, Christine Chaumeil and Lilia Merabet, in Pointe-à-Pitre, Joelle Claudéon, Elodie Curlier, Valérie Galantine, Jean Claude Gallois, Samuel Markowicz, Muriel Nicolas, Pascal Musson, and Kinda Schepers, in Poitiers, Alida Minoza and Catherine Kauffmann-Lacroix, in Rennes, Hélène Guégan, in Rouen, Damien Costa, Marion Dehais, and Gilles Gargala, in Reims, Odile Bajolet, Firouze Banisadr, Joel Cousson, Chantal Himberlin, Antoine Huguenin, and Dominique Toubas, in Saint Etienne, Pierre Flori, Caroline Mahinc, and Hélène Raberin, in Strabourg, Julie Denis, Raoul Herbrecht, Paul-Michel Mertes, Fehrat Meziani, Marcela Sabou, and Francis Schneider, in Toulouse, Anne-Isabelle Bertozzi, Pamela Chauvin, Elena Charpentier, Olivier Cointault, Claire Cottrel, Karen Delavigne, Stanislas Faguer, Judith Fillaux, Emilie Guemas, Xavier Iriart, Lucie Lelièvre, and Jean Ruiz, in Tours, Frédéric Bastides, Adélaïde Chesnay, and Guillaume Desoubeaux, in Versailles, Audrey Therby, and in Villejuif, Elisabeth Chachaty and Bertrand Gachot., and CLEMENT, Nathalie

Subjects
Antifungal Agents, pneumocytosis, invasive fungal infections, Pneumonia, Pneumocystis, [SDV]Life Sciences [q-bio], candidemia, Microbiology, [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, mucormycosis, [SDV] Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Virology, Humans, aspergillosis, epidemiology, Watchful Waiting, Fungemia, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Aged
Abstract

International audience; The French National Reference Center for Invasive Mycoses and Antifungals leads an active and sustained nationwide surveillance program on probable and proven invasive fungal diseases (IFDs) to determine their epidemiology in France. Between 2012 and 2018, a total of 10,886 IFDs were recorded. The incidence increased slightly over time (2.16 to 2.36/10,000 hospitalization days, P = 0.0562) in relation with an increase of fungemia incidence (1.03 to 1.19/10,000, P = 0.0023), while that of other IFDs remained stable. The proportion of ≥65-year-old patients increased from 38.4% to 45.3% (P < 0.0001). Yeast fungemia (n = 5,444) was due mainly to Candida albicans (55.6%) with stable proportions of species over time. Echinocandins became the main drug prescribed (46.7% to 61.8%), but global mortality rate remained unchanged (36.3% at 1 month). Pneumocystis jirovecii pneumonia (n = 2,106) was diagnosed mostly in HIV-negative patients (80.7%) with a significantly higher mortality than in HIV-positive patients (21.9% versus 5.4% at 1 month, P < 0.0001). Invasive aspergillosis (n = 1,661) and mucormycosis (n = 314) were diagnosed mostly in hematology (>60% of the cases) with a global mortality rate of 42.5% and 59.3%, respectively, at 3 months and significant changes in diagnosis procedure over time. More concurrent infections were also diagnosed over time (from 5.4% to 9.4% for mold IFDs, P = 0.0115). In conclusion, we observed an aging of patients with IFD with a significant increase in incidence only for yeast fungemia, a trend toward more concurrent infections, which raises diagnostic and therapeutic issues. Overall, global survival associated with IFDs has not improved despite updated guidelines and new diagnostic tools.IMPORTANCE The epidemiology of invasive fungal diseases (IFDs) is hard to delineate given the difficulties in ascertaining the diagnosis that is often based on the confrontation of clinical and microbiological criteria. The present report underlines the interest of active surveillance involving mycologists and clinicians to describe the global incidence and that of the main IFDs. Globally, although the incidence of Pneumocystis pneumonia, invasive aspergillosis, and mucormycosis remained stable over the study period (2012 to 2018), that of yeast fungemia increased slightly. We also show here that IFDs seem to affect older people more frequently. The most worrisome observation is the lack of improvement in the global survival rate associated with IFDs despite the increasing use of more sensitive diagnostic tools, the availability of new antifungal drugs very active in clinical trials, and a still low/marginal rate of acquired in vitro resistance in France. Therefore, other tracks of improvement should be investigated actively.

Published
2022

16. C’est dommage cette odeur

Author

Thomas Lhossein, Anne Totet, Taieb Chouaki, Jean-Philippe Bouchara, and Yohann Le Govic

Subjects
Medical Laboratory Technology, Biochemistry (medical), Analytical Chemistry
Published
2022

17. ‘Seven shades of Cryptosporidium’

Author

Crestia, Justine, primary, Razakandrainibe, Romy, additional, Costa, Damien, additional, Damiani, Céline, additional, Totet, Anne, additional, and Le Govic, Yohann, additional

Published
2022
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19. Design, synthesis, and characterization of novel aminoalcohol quinolines with strong in vitro antimalarial activity

Author

Catherine Mullié, Mathieu Marchivie, Patrice Agnamey, Anne Totet, Bruno Pradines, Anita Cohen, Alexandra Dassonville-Klimpt, Jérôme Dormoi, Jean Guillon, Nadine Azas, Céline Damiani, Pascal Sonnet, Jeremy Schneider, N. Taudon, C. Tisnerat, Toulin, Stéphane, Agents infectieux, résistance et chimiothérapie - UR UPJV 4294 (AGIR ), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Acides Nucléiques : Régulations Naturelle et Artificielle (ARNA), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Biomédicale des Armées (IRBA), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Centre National de Référence du Paludisme [IRBA, Marseille] (CNRP), Institut de Recherche Biomédicale des Armées [Antenne Marseille] (IRBA)-Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), The authors would like to thank Philippe Grellier, department RDDM at Muséum National d’Histoire Naturelle (Paris, France), for providing generously the 3D7 and W2 P. falciparum strains and Sylvie Klieber and Alain Pellet, Sanofi R et D France, for in vitro ADMET study (Papp, hERG activity, microsomal and hepatic clearance and CY3A4 activity)., Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), and ANR-21-ASM1-0003,MORHOC'H 2,Modélisation de l'interaction de la houle et d'un courant inhomogène en zone côtière(2021)

Subjects
Plasmodium falciparum, P. falciparum, mefloquine analogs, Cell Line, 03 medical and health sciences, Antimalarials, Mice, Structure-Activity Relationship, Cricetulus, Pharmacokinetics, Parasitic Sensitivity Tests, In vivo, Drug Discovery, Ic50 values, Animals, Humans, antimalarial drug resistance, aminoalcohol quinolines, 030304 developmental biology, Pharmacology, 0303 health sciences, Mice, Inbred BALB C, [CHIM.MATE] Chemical Sciences/Material chemistry, Dose-Response Relationship, Drug, Molecular Structure, 030306 microbiology, Chemistry, Organic Chemistry, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, Combinatorial chemistry, Amino Alcohols, In vitro, Ether-A-Go-Go Potassium Channels, 3. Good health, Malaria, Design synthesis, Novel agents, Drug Design, Microsomes, Liver, Quinolines, Female, Selectivity
Abstract

International audience; Malaria is the fifth most lethal parasitic infections in the world. Herein, five new series of aminoalcohol quinolines including fifty-two compounds were designed, synthesized and evaluated in vitro against Pf3D7 and PfW2 strains. Among them, fourteen displayed IC50 values below or near of 50.0 nM whatever the strain with selectivity index often superior to 100. 17b was found as a promising antimalarial candidate with IC50 values of 14.9 nM and 11.0 nM against respectively Pf3D7 and PfW2 and a selectivity index higher than 770 whatever the cell line is. Further experiments were achieved to confirm the safety and to establish the preliminary ADMET profile of compound 17b before the in vivo study performed on a mouse model of P. berghei ANKA infection. The overall data of this study allowed to establish new structure-activity relationships and the development of novel agents with improved pharmacokinetic properties

Published
2022

22. Reply to Gómez-Marín

Author

Ajzenberg, Daniel, Yera, Hélène, Marty, Pierre, Paris, Luc, Dalle, Frédéric, Menotti, Jean, Aubert, Dominique, Franck, Jacqueline, Bessières, Marie-Hélène, Quinio, Dorothée, Pelloux, Hervé, Delhaes, Laurence, Desbois, Nicole, Thulliez, Philippe, Robert-Gangneux, Florence, Kauffmann-Lacroix, Catherine, Pujol, Sophie, Rabodonirina, Meja, Bougnoux, Marie-Elisabeth, Cuisenier, Bernadette, Duhamel, Chantal, Duong, Thanh Hai, Filisetti, Denis, Flori, Pierre, Gay-Andrieu, Françoise, Pratlong, Francine, Nevez, Gilles, Totet, Anne, Carme, Bernard, Dardé, Marie-Laure, and Villena, Isabelle

Published
2009

23. Genotype of 88 Toxoplasma gondii Isolates Associated with Toxoplasmosis in Immunocompromised Patients and Correlation with Clinical Findings

Author

Ajzenberg, Daniel, Yera, Hélène, Marty, Pierre, Paris, Luc, Dalle, Frédéric, Menotti, Jean, Aubert, Dominique, Franck, Jacqueline, Bessières, Marie-Hélène, Quinio, Dorothée, Pelloux, Hervé, Delhaes, Laurence, Desbois, Nicole, Thulliez, Philippe, Robert-Gangneux, Florence, Kauffmann-Lacroix, Catherine, Pujol, Sophie, Rabodonirina, Meja, Bougnoux, Marie-Elisabeth, Cuisenier, Bernadette, Duhamel, Chantal, Duong, Thanh Hai, Filisetti, Denis, Flori, Pierre, Gay-Andrieu, Françoise, Pratlong, Francine, Nevez, Gilles, Totet, Anne, Carme, Bernard, Bonnabau, Henri, Dardé, Marie-Laure, and Villena, Isabelle

Published
2009
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24. « Une histoire d’éperon »

Author

Céline Damiani, Chloé Saint, Yohann Le Govic, Jérôme Boissier, and Anne Totet

Subjects
0303 health sciences, 03 medical and health sciences, Medical Laboratory Technology, 0302 clinical medicine, 030231 tropical medicine, Biochemistry (medical), Biology, 030304 developmental biology, Analytical Chemistry
Published
2021

27. Epidemiological and clinical study of microsporidiosis in French kidney transplant recipients from 2005 to 2019: TRANS‐SPORE registry

Author

Dumond, Clément, Aulagnon, Florence, Etienne, Isabelle, Heng, Anne‐Elisabeth, Bougnoux, Marie‐Elisabeth, Favennec, Loic, Kamar, Nassim, Iriart, Xavier, Pereira, Bruno, Büchler, Mathias, Desoubeaux, Guillaume, Kaminski, Hannah, Lussac‐Sorton, Florian, Gargala, Gilles, Anglicheau, Dany, Poirier, Philippe, Scemla, Anne, Garrouste, Cyril, Labbe, Franck, Damiani, Céline, Le Govic, Yohann, Totet, Anne, De Gentile, Ludovic, Lemoine, Jean‐Philippe, Bellanger, Anne Pauline, Accoceberry, Isabelle, Delhaes, Laurence, Gabriel, Frédéric, Millet, Pascal, Le Gal, Solène, Nevez, Gilles, Bonhomme, Julie, Capitaine, Agathe, Demar, Magalie, Moniot, Maxime, Nourrisson, Céline, Angebault, Cécile, Botterel, Françoise, Foulet, Françoise, Basmaciyan, Louise, Dalle, Frédéric, Valot, Stéphane, Desbois‐Nogard, Nicole, Robert, Gladys, Deleplancque, Anne‐Sophie, Frealle, Emilie, Leroy, Jordan, Ajzenberg, Daniel, Durieux, Marie‐Fleur, Chapey‐Picq, Emmanuelle, Dupont, Damien, Menotti, Jean, Rabodonirina, Meja, Lollivier, Coralie, Bastien, Patrick, Debourgogne, Anne, Machouart, Marie, Lavergne, Rose‐Anne, Morio, Florent, Delaunay, Pascal, Pomares, Christelle, Simon, Loïc, Sasso, Milène, Argy, Nicolas, Houze, Sandrine, Yera, Hélène, Dannaoui, Eric, Sitterle, Emilie, Kapel, Nathalie, Tantaoui, Ilhame, Thellier, Marc, Belkadi, Ghania Belkacem, Moreno‐Sabater, Alicia, Hamane, Samia, Nicolas, Muriel, Perraud, Estelle, Chemla, Cathy, Villena, Isabelle, Autier, Brice, Costa, Damien, Flori, Pierre, Brunet, Julie, BERRY, Antoine, Chesnay, Adélaïde, Artur, Fabienne, Verdurme, Laura, Foulquier, Jean‐Baptiste, Prigent, Gwenole, Chatelain, Rémi, Lesthelle, Sophie, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), SFR UA 4208 Interactions Cellulaires et Applications Thérapeutiques (ICAT), Laboratoire de Parasitologie et Mycologiede [CHRU Brest], Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), CHU Clermont-Ferrand, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Rouen, Normandie Université (NU), Institut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), CHU Trousseau [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), CHU Bordeaux [Bordeaux], Infection Inflammation et Interaction Hôtes Pathogènes [CHU Clermont-Ferrand] (3IHP ), Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), CHU Clermont-Ferrand-CHU Clermont-Ferrand, Université Clermont Auvergne (UCA), Centre Hospitalier Universitaire de Reims (CHU Reims), Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Université de Reims Champagne-Ardenne (URCA)-Université de Rouen Normandie (UNIROUEN), and Normandie Université (NU)-Normandie Université (NU)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)

Subjects
Male, medicine.medical_specialty, Opportunistic infection, [SDV]Life Sciences [q-bio], 030230 surgery, Microsporidiosis, Gastroenterology, Albendazole, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, parasitic diseases, medicine, Humans, Enterocytozoon bieneusi, Fumagillin, Registries, Kidney transplantation, ComputingMilieux_MISCELLANEOUS, Aged, 0303 health sciences, Transplantation, biology, 030306 microbiology, business.industry, Enterocytozoon, Middle Aged, Spores, Fungal, fumagillin, medicine.disease, biology.organism_classification, Kidney Transplantation, 3. Good health, immunosuppressive regimen, Diarrhea, Infectious Diseases, medicine.symptom, business, medicine.drug
Abstract

Introduction Microsporidiosis is an emerging opportunistic infection in renal transplantation (RT) recipients. We aimed to describe its clinical presentation and treatment. Materials and methods We collected microsporidiosis cases identified in RT recipients between 2005 and 2019 in six French centers from the Crystal, Divat and Astre prospective databases. Results We report 68 RT recipients with intestinal microsporidiosis; the patients were predominantly male (61.8%), with a median age of 58 (46-69) years. Infection occurred at a median time of 3 (0.8-6.8) years posttransplant. Only Enterocytozoon bieneusi was found. Microsporidiosis manifested as diarrhea (98.5% of patients) with weight loss (72.1%) and acute renal injury (57.4%) without inflammatory biological parameters. The therapeutic approaches were no treatment (N = 9), reduction of the immunosuppressive regimen (∆IS) (N = 22), fumagillin alone (N = 9), fumagillin and ∆IS (N = 19), and albendazole or nitazoxanide and ∆IS (N = 9). Overall clinical remission was observed in 60 patients (88.2%). We observed no acute kidney rejection, renal transplant failure or death within 6 months after microsporidiosis. Conclusion E. bieneusi is an underestimated opportunistic pathogen in RT recipients, and infection with E. bieneusi leads to diarrhea with important dehydration and acute renal injury. The treatment is based on the reduction of the immunosuppressive regimen and the administration of fumagillin if available. This article is protected by copyright. All rights reserved.

Published
2021

29. A Negative (1,3)-β-D-Glucan Result Alone Is Not Sufficient to Rule Out a Diagnosis of Pneumocystis Pneumonia in Patients With Hematological Malignancies

Author

Céline Damiani, Baptiste Demey, Cécile Pauc, Yohann Le Govic, Anne Totet, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), and SFR UA 4208 Interactions Cellulaires et Applications Thérapeutiques (ICAT)

Subjects
Microbiology (medical), medicine.medical_specialty, [SDV]Life Sciences [q-bio], Pneumocystis pneumonia, Microbiology, Gastroenterology, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Pneumocystis jirovecii, In patient, 030212 general & internal medicine, (1,3)-β-D-glucan, 0303 health sciences, bronchoalveolar lavage fluid, biology, 030306 microbiology, business.industry, medicine.disease, biology.organism_classification, Predictive value, QR1-502, 3. Good health, Hematological malignancy, biomarker, Biomarker (medicine), HIV-negative patients, business, 1 3 β d glucan, Inflammatory disorder
Abstract

Background: Serum (1,3)-β-D-glucan (BG) testing is increasingly being used in the diagnostic armamentarium for invasive fungal diseases. Given its high sensitivity, some studies suggest that a negative BG result contributes to rule out a diagnosis of Pneumocystis pneumonia (PCP). However, recent reports described a suboptimal sensitivity in HIV-negative immunocompromised patients. In this study, we evaluated the performance of BG assay for PCP diagnosis in HIV-negative patients with diverse PCP risk factors. We also assessed the correlation between Pneumocystis jirovecii load in pulmonary samples and serum BG levels.Methods: We retrospectively included HIV-negative patients with microscopically proven PCP and for whom a BG result was available. We also enrolled patients colonized by Pneumocystis as control group. Colonized patients were matched with PCP patients based on their underlying condition that exposed to PCP. Pulmonary fungal loads were determined by an in-house real-time PCR, and BG levels were measured by using the Fungitell® kit (Associates of Cape Cod, Inc.).Results: Thirty-nine patients were included in each of the two groups. Thirty-four of 39 PCP patients and one of 39 colonized patient had a positive BG test, resulting in a sensitivity of 0.87 (95% CI: 0.73–0.94), a specificity of 0.97 (95% CI: 0.87–0.99), a positive predictive value of 0.97 (95% CI: 0.85–0.99), and a negative predictive value of 0.88 (95% CI: 0.75–0.95) for BG assay. Nonetheless, median BG level differed according to the underlying condition. Among the PCP group, the lowest median level of 211 pg/ml was observed in patients with hematological malignancy (HM) and differed significantly from that observed either in solid organ transplants (3,473 pg/ml) or in patients with autoimmune or inflammatory disorder (3,480 pg/ml). Indeed, the sensitivity of BG assay was estimated at 0.64 (95% CI: 0.35–0.85) in HM patients and was lower than the one observed in the whole PCP group. Furthermore, BG level and fungal burden correlated poorly among all PCP patients.Conclusion: BG is not a reliable biomarker for ruling out PCP in HIV-negative patients with HM. Interpretation of a negative BG result should take into account, but not be limited to, the underlying condition predisposing to PCP.

Published
2021

30. ‘Seven shades of Cryptosporidium’

Author

Damien Costa, Yohann Le Govic, Romy Razakandrainibe, Céline Damiani, Anne Totet, Justine Crestia, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), and SFR UA 4208 Interactions Cellulaires et Applications Thérapeutiques (ICAT)

Subjects
0301 basic medicine, Microbiology (medical), [SDV]Life Sciences [q-bio], 030106 microbiology, Cryptosporidiosis, Cryptosporidium, Feces, 03 medical and health sciences, 0302 clinical medicine, Coccidia, parasitic diseases, medicine, Humans, 030212 general & internal medicine, ComputingMilieux_MISCELLANEOUS, biology, Zoonosis, General Medicine, Gold standard (test), biology.organism_classification, medicine.disease, Negative stain, Virology, 3. Good health, Staining, Infectious Diseases
Abstract

In this paper, we describe a case of clinically unsuspected cryptosporidiosis that was diagnosed through systematic screening of coccidia using the little known, but highly reliable, extemporaneous Heine technique. This is significant because this zoonosis is largely underestimated, probably since most of the laboratories may not look for Cryptosporidium unless specifically requested. Moreover, the performance of the modified Ziehl-Neelsen staining, still considered as “gold standard”, remains variable owing to the increasing proportion of non-acid-fast oocysts “ghosts” in resolving infections. Contrastingly, the negative staining technique of Heine highlights oocysts irrespectively of the time since diarrhoea onset. In addition, we provide a rare panel of fluorescent images that would be useful for microscopists.

Published
2022

33. Pneumocystis jiroveci Dihydropteroate Synthase Genotypes in Immunocompetent Infants and Immunosuppressed Adults, Amiens, France

Author

Anne Totet, Sophie Latouche, Philippe Lacube, Jean-Claude Pautard, Vincent Jounieaux, Christian Raccurt, Patricia Roux, and Gilles Nevez

Subjects
Pneumocystis, human, infant, bronchiolitis, reservoir, circulation, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

To date, investigations of Pneumocystis jiroveci circulation in the human reservoir through the dihydropteroate synthase (DHPS) locus analysis have only been conducted by examining P. jirovecii isolates from immunosuppressed patients with Pneumocystis pneumonia (PCP). Our study identifies P. jirovecii genotypes at this locus in 33 immunocompetent infants colonized with P. jirovecii contemporaneously with a bronchiolitis episode and in 13 adults with PCP; both groups of patients were monitored in Amiens, France. The results have pointed out identical features of P. jirovecii DHPS genotypes in the two groups, suggesting that in these two groups, transmission cycles of P. jirovecii infections are linked. If these two groups represent sentinel populations for P. jirovecii infections, our results suggest that all persons parasitized by P. jirovecii, whatever their risk factor for infection and the form of parasitism they have, act as interwoven circulation networks of P. jirovecii.

Published
2004
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36. BioQuiz : Parasitologie « Une histoire d'éperon »

Author

Saint, Chloé, Damiani, Céline, Boissier, Jérôme, Totet, Anne, Le Govic, Yohann, Laboratoire de parasitologie et de mycologie médicales [CHU Amiens], CHU Amiens-Picardie, Agents infectieux, résistance et chimiothérapie - UR UPJV 4294 (AGIR ), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Université de Perpignan Via Domitia (UPVD), Interactions Hôtes-Pathogènes-Environnements (IHPE), and Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD)

Subjects
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
Abstract

International audience; Description Un jeune garçon de 15 ans est hospitalisé pour malaise et perte de connaissance à la suite d'une dispute avec sa famille d'accueil temporaire. Le bilan étiologique complet, comprenant notamment un scanner cérébral injecté et un électroencéphalogramme, est sans particularité. Au cours de l'hospitalisation, on découvre une hématurie macroscopique, associée à des douleurs scrotales. Le reste de l'examen clinique est normal. Le patient est, par ailleurs, apyrétique et en parfait état général. À l'interrogatoire, on apprend qu'il est originaire de Côte d'Ivoire, qu'il vit le reste de l'année dans un foyer pour mineurs en Seine-Saint-Denis (93), et que son dernier séjour dans son pays natal remonte à cinq ans. Le bilan sanguin montre une légère hyperéosinophilie (1,1 G/L ; N inférieur à 0,5 G/L) sans aucune autre anomalie. Un examen cytobactériologique des urines confirme l'hématurie, accompagnée d'une leucocyturie. La culture est négative. Une échographie vésicale est pratiquée, montrant un épaississement pseudo-polypoïde isoéchogène sur la paroi postérieure de la vessie. Un examen parasitologique des urines est alors réalisé. Outre la présence d'hématies, plusieurs éléments attirent l'attention du microscopiste (figures 1A et 1B). La barre représente 50 μm.

Published
2021

37. Early diagnosis and monitoring of mucormycosis by detection of circulating DNA in serum: retrospective analysis of 44 cases collected through the French Surveillance Network of Invasive Fungal Infections (RESSIF)

Author

Dupont, H., Marolleau, J.P., Totet, A., Damiani, C., Berceanu, A., Larosa, F., Bonhomme, J., Chabrot, C., Bouteille, B., Boutoille, D., Gastinne, T., Peterlin, P., Gari Toussaint, M., Poisson, D., Briet, D., Buret, J., Legrand, M., Denis, B., Raffoux, E., Bergeron, A., Veinstein, A., Godet, C., N'guyen, Y., Diallo, S., Sabou, M., Denis, J., Ledoux, M.P., Recher, C., Ruiz, J., Desoubeaux, G., Bailly, E., Chachaty, E., Dromer, F., Lortholary, O., Sitbon, K., Hoinard, D., Millon, L., Herbrecht, R., Grenouillet, F., Morio, F., Alanio, A., Letscher-Bru, V., Cassaing, S., Chouaki, T., Kauffmann-Lacroix, C., Poirier, P., Toubas, D., Augereau, O., Rocchi, S., Garcia-Hermoso, D., and Bretagne, S.

Published
2016
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39. It is still PCP that can stand for Pneumocystis pneumonia: Appeal for generalized use of only one acronym

Author

Solène Le Gal, Gilles Nevez, Olga Matos, Robert F. Miller, Anne Totet, Enrique J. Calderón, Groupe d'Étude des Interactions Hôte-Pathogène (GEIHP), Université d'Angers (UA), SFR UA 4208 Interactions Cellulaires et Applications Thérapeutiques (ICAT), Laboratoire de Parasitologie et Mycologiede [CHRU Brest], Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), and Universidad de Sevilla. Departamento de Medicina

Subjects
0301 basic medicine, [SDV]Life Sciences [q-bio], Pneumocystis pneumonia, 03 medical and health sciences, 0302 clinical medicine, Terminology as Topic, parasitic diseases, medicine, Pneumocystis jirovecii, Abbreviations as Topic, Acronym, biology, Pneumocystis, business.industry, Pneumonia, Pneumocystis, PNEUMOCYSTIS JIROVECI, General Medicine, medicine.disease, biology.organism_classification, Virology, 3. Good health, respiratory tract diseases, PCP, Pneumocystis species, 030104 developmental biology, Infectious Diseases, Pneumocystis carinii, 030220 oncology & carcinogenesis, business, Host specificity
Abstract

Twenty-years ago, considering the host specificity of Pneumocystis species, the human-derived Pneumocystis, Pneumocystis carinii formae specialis hominis, was renamed Pneumocystis jirovecii. Pneumocystis carinii formae specialis carinii was finally renamed Pneumocystis carinii and kept for the species derived from Rattus norvegicus. P. jirovecii is now widely used by most authors. The PCP acronym that initially referred to “Pneumocystis cariniipneumonia” was contemporaneously redefined to stand for Pneumocystispneumonia in order to avoid changing the acronym of the name of the disease that clinicians have used for several decades. Using analysis of multidata bases on PubMed, we have noted a recent acceleration in the use of PJP for Pneumocystis jiroveciipneumonia, which may be grammatically correct but not in accordance with retaining PCP, which was proposed in the early 2000s. Through this reminder, in order to standardize the literature on P. jirovecii, we plead for the use of only one acronym, PCP. Lay Summary Through this reminder on Pneumocystis nomenclature, we plead for the use of only one acronym, PCP, the retention of which was proposed in the early 2000s, and which currently stands for Pneumocystispneumonia.

Published
2021

40. It is still PCP that can stand for Pneumocystis pneumonia: appeal for generalized use of only one acronym

Author

Universidad de Sevilla. Departamento de Medicina, Nevez, Gilles, Totet, Anne, Matos, Olga, Calderón Sandubete, Enrique José, Miller, Robert F., Le Gal, Solène, Universidad de Sevilla. Departamento de Medicina, Nevez, Gilles, Totet, Anne, Matos, Olga, Calderón Sandubete, Enrique José, Miller, Robert F., and Le Gal, Solène

Abstract

Twenty-years ago, considering the host specificity of Pneumocystis species, the human-derived Pneumocystis, Pneumocystis carinii formae specialis hominis, was renamed Pneumocystis jirovecii. Pneumocystis carinii formae specialis carinii was finally renamed Pneumocystis carinii and kept for the species derived from Rattus norvegicus. P. jirovecii is now widely used by most authors. The PCP acronym that initially referred to “Pneumocystis cariniipneumonia” was contemporaneously redefined to stand for Pneumocystispneumonia in order to avoid changing the acronym of the name of the disease that clinicians have used for several decades. Using analysis of multidata bases on PubMed, we have noted a recent acceleration in the use of PJP for Pneumocystis jiroveciipneumonia, which may be grammatically correct but not in accordance with retaining PCP, which was proposed in the early 2000s. Through this reminder, in order to standardize the literature on P. jirovecii, we plead for the use of only one acronym, PCP.

Published
2021

41. It is still PCP that can stand for Pneumocystis pneumonia: Appeal for generalized use of only one acronym

Author

Nevez, Gilles, Totet, Anne, Matos, Olga, Calderón, Enrique J., Miller, Robert F., Le Gal, Solène, Nevez, Gilles, Totet, Anne, Matos, Olga, Calderón, Enrique J., Miller, Robert F., and Le Gal, Solène

Abstract

Twenty-years ago, considering the host specificity of Pneumocystis species, the human-derived Pneumocystis, Pneumocystis carinii formae specialis hominis, was renamed Pneumocystis jirovecii. Pneumocystis carinii formae specialis carinii was finally renamed Pneumocystis carinii and kept for the species derived from Rattus norvegicus. P. jirovecii is now widely used by most authors. The PCP acronym that initially referred to “Pneumocystis cariniipneumonia” was contemporaneously redefined to stand for Pneumocystispneumonia in order to avoid changing the acronym of the name of the disease that clinicians have used for several decades. Using analysis of multidata bases on PubMed, we have noted a recent acceleration in the use of PJP for Pneumocystis jiroveciipneumonia, which may be grammatically correct but not in accordance with retaining PCP, which was proposed in the early 2000s. Through this reminder, in order to standardize the literature on P. jirovecii, we plead for the use of only one acronym, PCP.

Published
2021

43. « Oh les jolis veaux ! »

Author

Yohann Le Govic, Damien Costa, Anne Totet, Justine Crestia, and Céline Damiani

Subjects
Medical Laboratory Technology, business.industry, Biochemistry (medical), Medicine, business, Analytical Chemistry
Published
2021

44. Invasive Tracheobronchial Aspergillosis in Critically Ill Patients with Severe Influenza. A Clinical Trial

Author

Saad Nseir, Anne Totet, Malcolm Lemyze, Michel Slama, Elie Zogheib, Ivona Milic, Rémy Nyga, Hervé Dupont, Pierre-Alexandre Roger, Sandrine Castelain, Julien Maizel, Nicolas Van Grunderbeeck, Boualem Sendid, Taieb Chouaki, CHU Amiens-Picardie, Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Laboratoire de parasitologie et de mycologie médicales [CHU Amiens], Centre Hospitalier de Lens, Agents infectieux, résistance et chimiothérapie - UR UPJV 4294 (AGIR ), Laboratoire de Virologie [CHU Amiens], Simplification des soins chez les patients complexes - UR UPJV 7518 (SSPC), Université de Picardie Jules Verne (UPJV), Service de Parasitologie-Mycologie [CHRU LIlle], Institut de Microbiologie [CHRU Lille], Pôle de Biologie Pathologie Génétique [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Pôle de Biologie Pathologie Génétique [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Institut Pasteur de Lille, and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)

Subjects
Pulmonary and Respiratory Medicine, medicine.medical_specialty, [SDV]Life Sciences [q-bio], macromolecular substances, Critical Care and Intensive Care Medicine, Aspergillosis, Severe influenza, 03 medical and health sciences, Galactomannan, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, medicine, aspergillosis, 030212 general & internal medicine, (1-3)-β-d-glucan, business.industry, Critically ill, Invasive pulmonary aspergillosis, medicine.disease, 3. Good health, Clinical trial, 030228 respiratory system, chemistry, galactomannan, ICU, business, influenza, 1 3 β d glucan
Abstract

International audience; Rationale: Invasive tracheobronchial aspergillosis (ITBA) is an uncommon but severe clinical form of invasive pulmonary aspergillosis in which the fungal infection is entirely or predominantly confined to the tracheobronchial tree.Objectives: To analyze the diagnostic and prognostic differences between tracheobronchial aspergillosis and pulmonary aspergillosis without tracheobronchial lesions among patients admitted to the ICU with severe influenza.Methods: This retrospective, observational study included critically ill patients with influenza associated with pulmonary aspergillosis from three hospital ICUs between 2010 and 2019. Patient characteristics and clinical and mycologic data at admission and during ICU stay were collected in a database to evaluate variables in the two groups.Measurements and Main Results: Thirty-five patients admitted to the ICU with severe influenza and pulmonary aspergillosis were included. Ten patients were included in the group with ITBA (n = 10 of 35; 28.6%), and 25 patients were included in the group without ITBA. The group with ITBA comprised more patients with active smoking, diabetes mellitus, and higher severity scores (Simplified Acute Physiology Score II). Ninety-day mortality rates in the groups with and without ITBA were 90% and 44%, respectively (P = 0.02). Moreover, significantly higher serum 1,3-β-d-glucan and galactomannan and BAL fluid galactomannan concentrations were observed in the group with ITBA compared with the group without ITBA (P

Published
2020

45. Cross-Sectional Serological Survey of Human Fascioliasis in Haiti

Author

P. Agnamey, E. Fortes-Lopes, C. P. Raccurt, J. Boncy, and A. Totet

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Fasciola hepatica, the aetiological agent of fascioliasis in the Caribbean region, occurs throughout the major islands of the Greater Antilles and in localised zones on two islands (Martinique and Saint Lucia) of the Lesser Antilles. However, apart from Puerto Rico, information regarding human fascioliasis in islands of the Caribbean is out of date or unavailable, or even nonexistent as in Haiti. The authors conducted a retrospective, cross-sectional serological survey in Port-au-Prince using a Western blotting test (LDBIO Diagnostics) on human fascioliasis in Haiti. A total of 216 serum samples obtained from apparently healthy adults were tested. The frequency of antibodies in serum samples of the study population was 6.5% (14/216). The immunodominant bands recognised in Western blots were 27-28 kDa (100%), 42 kDa (64%), 60 kDa, and 8-9 kDa (28%). This is the first survey to reveal a relatively low proportion of asymptomatic F. hepatica-infected humans in Haiti.

Published
2012
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46. Childhood Cryptosporidiosis: A Case Report

Author

P. Agnamey, D. Djeddi, A. Diallo, A. Vanrenterghem, N. Brahimi, C. da Costa, and A. Totet

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Cryptosporidium has emerged as an important cause of diarrheal illness worldwide, especially amongst young children and patients with infectious or iatrogenic immune deficiencies. The authors describe a case of mild cryptosporidiosis in a well-nourished, immunocompetent, one-year-old child. Rapid clinical and parasitological improvement was observed after a 3-day course of nitazoxanide.

Published
2010
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48. Similar genotypes of Pneumocystis jirovecii in different forms of pneumocystis infection

Author

Totet, Anne, Duwat, Hyacinthe, Magois, Eline, Jounieaux, Vincent, Roux, Patricia, Raccurt, Christian, and Nevez, Gilles

Subjects
Pneumocystis carinii pneumonia -- Research, Pneumocystis carinii pneumonia -- Care and treatment, Genotype -- Research, Biological sciences
Abstract

This study describes the genotyping of Pneumocystis jirovecii organisms isolated from three groups of patients that developed diverse forms of P. jirovecii infection; the patients were monitored in the same French hospital. Forty archival specimens from 13 adults with Pneumocystis pneumonia, eight adults colonized by P. jirovecii and 19 immunocompetent infants infected with the fungus contemporaneously with a bronchiolitis episode were analysed retrospectively. Genotyping was performed by analysis of sequences of the internal transcribed spacer (ITS)1 and ITS2 regions, and of the dihydropteroate synthase (DHPS) locus. At the ITS regions, a high diversity of genotypes, identical main genotypes ([B.sub.1][a.sub.3] and [B.sub.2][a.sub.1]) and the occurrence of mixed infections (more than one genotype) were observed in the three patient groups. At the DHPS locus, the results indicated the presence of mutants in the two adult groups, as well as in the infant group. Consequently, at these two independent genomic regions, P. jirovecii isolates from patients who developed different forms of infection and who lived in the same geographical region presented common characteristics. These results suggest that patients infected with P. jirovecii, whatever the form of infection they present, are part of a common human reservoir for P. jirovecii.

Published
2004

49. Pneumocystis jirovecii dihydropteroate synthase genotypes in immunocompetent infants and immunosuppressed adults, Amiens, France

Author

Totet, Anne, Latouche, Sophie, Lacube, Philippe, Pautard, Jean-Claude, Jounieaux, Vincent, Raccurt, Christian, Roux, Patricia, and Nevez, Gilles

Subjects
Pneumonia -- Research, Bacterial pneumonia -- Research
Abstract

To date, investigations of Pneumocystis jirovecii circulation in the human reservoir through the dihydropteroate synthase (DHPS) locus analysis have only been conducted by examining P. jirovecii isolates from immunosuppressed patients [...]

Published
2004

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