1. A glutathione disulfide-sensitive Janus nanomachine controlled by an enzymatic and logic gate for smart delivery
- Author
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Mayol Hornero, Beatriz, Díez-Sánchez, Paula, Sánchez Sánchez, Alfredo, Torre, Cristina de la, Villalonga, Anabel, Lucena-Sánchez, Elena, Sancenón, Félix, Martínez Ruiz, María Paloma, Vilela García, Diana, Martínez-Máñez, Ramón, Villalonga Santana, Reynaldo, Mayol Hornero, Beatriz, Díez-Sánchez, Paula, Sánchez Sánchez, Alfredo, Torre, Cristina de la, Villalonga, Anabel, Lucena-Sánchez, Elena, Sancenón, Félix, Martínez Ruiz, María Paloma, Vilela García, Diana, Martínez-Máñez, Ramón, and Villalonga Santana, Reynaldo
- Abstract
This work describes the assembly of a novel enzyme-controlled nanomachine operated through an AND Boolean logic gate for on-command delivery. The nanodevice was constructed on Au-mesoporous silica Janus nanoparticles capped with a thiol-sensitive gate-like molecular ensemble on the mesoporous face and functionalized with glutathione reductase on the gold face. This autonomous nanomachine employed NADPH and glutathione disulfide as input chemical signals, leading to the enzymatic production of reduced glutathione that causes the disruption of the gating mechanism on the mesoporous face and the consequent payload release as an output signal. The nanodevice was successfully used for the autonomous release of doxorubicin in HeLa cancer cells and RAW 264.7 macrophage cells., Spanish Ministry of Economy and Competitiveness, Generalitat Valenciana, Depto. de Química Analítica, Fac. de Ciencias Químicas, TRUE, pub
- Published
- 2024