Search

Selenium is a well‐known element that can be toxic and potentially harmful in excessive amounts. In this study, the microwave‐assisted synthesis of selenium nanoparticles (SeNP) was achieved using glucose as a green reductant, resulting in reduced toxicity. This method provides a rapid, cost‐effective, and environmentally friendly alternative to traditional SeNP synthesis techniques. The antioxidant activity of the SeNPs was assessed using the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay, while their antibacterial properties were tested against both Gram‐positive (E. coli) and Gram‐negative (S. aureus) bacteria using the agar diffusion method. Additionally, the cytotoxicity of SeNPs towards the Beas2B cell line was evaluated using the MTT viability assay at various concentrations. Results produced confirm the successful synthesis of SeNPs with notable antioxidant and antibacterial properties through a simple and economical approach, indicating potential applications in various fields. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Polytechnic is the property of Journal of Polytechnic and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Wound healing is a topic of significant interest in current times, owing to the escalating incidence of chronic diseases associated with impaired healing, as well as the growing number of elderly individuals within the population. Amongst the various approaches for fabrication of wound healing dressings, the utilization of selenium‐based nanoparticles has garnered considerable attention due to selenium's numerous advantages, including antioxidant, antiviral, antibacterial, and antifungal activities. With this perspective, we focused on the fabrication and characterization of hydrogels incorporated with selenium nanoparticles (SeNPs). In this work, we have developed a microwave‐assisted hydrothermal synthesis strategy for synthesis of the SeNPs that employ non‐toxic precursors, thereby reducing the risk of environmental toxicity and providing a cost‐effective alternative to conventional chemical and hydrothermal methods. Subsequently, we have successfully incorporated SeNPs into hyaluronic acid methacrylate/gelatin methacrylate‐based hydrogels. Hyaluronic acid and gelatin are selected to support the healing process further, and these polymers are methacrylated in order to further control mechanical properties of the hydrogel and improve the stability of the dressing. The nanoparticles and the nanoparticle‐incorporated hydrogels were characterized by various techniques including Fourier transform infrared spectroscopy, UV–Vis spectroscopy, scanning electron microscopy, and dynamic light scattering instruments. Mechanical behaviors, swelling and degradation properties of the dressings were evaluated. Afterwards, we have conducted cell culture studies with SeNPs‐loaded hydrogels to determine the efficacy of SeNPs in wound healing. According to experimental findings, in vitro scratch assay suggests that a hydrogel dressing containing SeNPs (HG‐SeNp2) support the cell migration more compared to other samples incorporated with nanoparticles and to the control study at 24 h, and the wound closure percentage was found to be statistically significant compared with the control study. This dressing hold promise as effective wound dressings that can facilitate and expedite the process of wound healing. [ABSTRACT FROM AUTHOR]

Antimicrobial peptides are the first defense molecules against various organisms such as bacteria, fungi and are produced by different organisms. In this study, we aimed to investigate the anticancer effects of an insect antimicrobial peptide, cryptonin isolated from the hemolymph of cicada, Cryptotympana dubia on malignant melanoma cells. Cryptonin decreased the cell viability in a dose and time-dependent manner and these effects are more in metastatic cells than in other cell lines. The release of LDH from damaged cells and the detection of HMGB1 in cell culture media after peptide treatment indicated that cryptonin could induce cell necrosis and this cell death may activate an immune response. The peptide also increased the necrotic cell nucleus stained with ethidium bromide. According to reactive oxygen species production and mitochondrial membrane depolarization results, the apoptotic effect of the peptide was low in melanoma cells. Although the peptide killed the keratinocytes in vitro, cryptonin or cryptonin- derived peptide may have potential use in the treatment of malignant melanoma. [ABSTRACT FROM AUTHOR]