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The study assessed petroleum systems in the southern Bida Basin, Nigeria, focusing on the Cretaceous sediments. Reservoirs comprise Lokoja and Patti Formation sandstones, while shales, claystone, and siltstone serve as source rocks and stratigraphic traps/seals. Detailed studies delineating the key elements of the petroleum systems in the basin have not been conducted due to a lack of subsurface data. The goal of the current study was to use field observations, Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), and geochemical and geomechanical data to evaluate key components of petroleum systems in the southern Bida Basin. The results presented in this investigation are intended to attain specific objectives, especially those lacking in the basin's study sections. Petrophysical analysis revealed that the Lokoja Sandstone has porosity of 37%–39.5% and moderate permeability of 23.22–42.29 mD. The Patti Formation sandstone reservoirs exhibited high porosity (38%–42%) and moderate to good permeability (31.54–66.48 mD), suggesting good potential reservoirs. SEM results revealed intragranular pores and micro-fractures in the Patti Shale, whereas the sandstone reservoirs in the Lokoja and Patti formations displayed fractures, facilitating hydrocarbon migration. Quartz was the dominant mineral in the sandstone units of both formations. XRD analysis revealed that brittle and clay minerals influenced the microstructure of Patti Shale. Geochemical analysis indicated promising petroleum potential in the Patti Shale, with a total organic carbon (TOC) content of 1.87 wt%, free hydrocarbon from kerogen (S1) of 0.41 mg/g, hydrogen index (HI) of 0.75 mg HC/g TOC, and production index (PI) of 0.17. The Patti and Lokoja formations' shale, claystone, and siltstone exhibited sealing potential, with a plasticity index of 24–35 and coefficient permeability of 2.8 × 10−4- 3.6 × 10−4 cm/s. Field studies, XRD, geochemical data, and geomechanical index values have confirmed the key components of the petroleum system, which in turn facilitate hydrocarbon generation, migration, accumulation, and entrapment in the basin.