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Objective: Lentinan (LNT) had been shown to have direct cytotoxicity on cancer cells. However, the mechanism of this anti-tumor effect was still unclear. The purpose of this study was to investigate the effect of lentinan on the proliferation, migration and chemosensitivity of pancreatic cancer cells and its mechanism. Methods: Capan-1 cells from pancreatic cancer were randomly divided into control group, IL-6 group and IL-6+LNT group. Cell survival rate was detected by MTT, expression levels of p-STAT3, STAT3, Notch1 and Hes1 were analyzed by Western blot, and cell migration was observed by Transwell test. The cisplatin resistant cell model (Capan-1/DDP) was established, and the semi-inhibitory concentration of cisplatin (IC50) was detected in each group, and the relative expression levels of Ki67, MMP-9, MRP1 and P-gp were analyzed. Capan-1/DDP cell line was selected for tumor formation test in nude mice to verify the effects of LNT on tumor weight and IL-6/STAT3/Notch signaling pathway in nude mice. Results: Lentinan (7.5~240 µg/mL) could inhibit proliferation of pancreatic cancer cells. Compared with the IL-6 group, the expression levels of p-STAT3/STAT3, Notch1 and Hes1 in IL-6+LNT group were significantly down-regulated, the proliferation rate and the migration level was significantly dcreased (P

Alzheimer’s disease is pathologically characterized by the presence of senile plaques and neurofibrillary tangles in the central nervous system. Amyloid β-protein is toxic to neurons and induces phosphorylation of Tau protein, which accumulates in paired helical filaments and leads to the formation of neurofibrillary tangles. This study is focused on the Wnt/β-catenin pathway influence on Tau phosphorylation and the distribution of microtubules and neurofilaments in adrenal pheochromocytoma cells. It was found that neurofilament heavy polypeptide and microtubule-associated protein-2 aggregated after treatment with Aβ1-42. Treatment with Wnt5a reduced this aggregation, while Dickkopf-1 treatment promoted microtubule and neurofilament aggregation. Furthermore, Tau phosphorylation at Ser396, Ser422, and Ser199 was significantly reduced after Wnt5a treatment, whereas Dickkopf-1 increased the level of phosphorylation. These results suggest that the Wnt/β-catenin pathway influences the distribution of microtubules and neurofilaments, possibly by modulating the phosphorylation of Tau protein in adrenal pheochromocytoma cells.