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Background and aims Nonalcoholic fatty liver disease, especially nonalcoholic steatohepatitis (NASH), has become a major cause of liver transplantation and liver-associated death. NASH is the hepatic manifestation of metabolic syndrome and is characterized by hepatic steatosis, inflammation, hepatocellular injury, and different degrees of fibrosis. However, there is no US Food and Drug Administration-approved medication to treat this devastating disease. Therapeutic activators of the AMP-activated protein kinase (AMPK) have been proposed as a potential treatment for metabolic diseases such as NASH. Cordycepin, a natural product isolated from the traditional Chinese medicine Cordyceps militaris, has recently emerged as a promising drug candidate for metabolic diseases. Approach and results We evaluated the effects of cordycepin on lipid storage in hepatocytes, inflammation, and fibrosis development in mice with NASH. Cordycepin attenuated lipid accumulation, inflammation, and lipotoxicity in hepatocytes subjected to metabolic stress. In addition, cordycepin treatment significantly and dose-dependently decreased the elevated levels of serum aminotransferases in mice with diet-induced NASH. Furthermore, cordycepin treatment significantly reduced hepatic triglyceride accumulation, inflammatory cell infiltration, and hepatic fibrosis in mice. In vitro and in vivo mechanistic studies revealed that a key mechanism linking the protective effects of cordycepin were AMPK phosphorylation-dependent, as indicated by the finding that treatment with the AMPK inhibitor Compound C abrogated cordycepin-induced hepatoprotection in hepatocytes and mice with NASH. Conclusion Cordycepin exerts significant protective effects against hepatic steatosis, inflammation, liver injury, and fibrosis in mice under metabolic stress through activation of the AMPK signaling pathway. Cordycepin might be an AMPK activator that can be used for the treatment of NASH.

The Missan oil field is located in southeastern Iraq and is an important part of Iraq's oil economy. Carbonate reservoirs in the Missan Oilfield have the characteristics of multiple mineral types, large changes in reservoir physical properties, and strong heterogeneity. It is difficult to develop reservoirs reasonably and effectively. Therefore, subsequent acidification and acid fracturing of the reservoir Reconstruction is necessary. To provide a basis for the selection of input parameters of the subsequent productivity prediction model and improve the effectiveness of acidified acid fracturing reservoir reconstruction, this paper prepares cast thin sections and uses an electron microscope to observe the microscopic pore structure of the thin sections before and after acid etching. The research results show that acid etching will significantly increase the storage space, and the dissolution rate and dissolved pore morphology of different types and shapes of minerals after exposure to acid have obvious differences. The accuracy has certain guiding significance.

Tight gas is currently an important area for the exploration and development of unconventional oil and gas resources at home and abroad. However, due to the low permeability and high-water saturation characteristics of tight gas reservoirs, gas wells exhibit low productivity, rapid decline in production, and water production in some wells. Such characteristics have reduced the single-well EUR to a certain extent, affecting the recovery rate of the gas reservoir. For new blocks, single-well EUR is the basic condition for long-term high and stable production of oil and gas wells. It is an important indicator for evaluating development effects, analyzing development potential, and formulating development-specific measures. How to quickly and accurately predict and analyze single-well EUR has always been tight Important research work for the development of gas. This paper takes the tight sandstone gas reservoir in the Bajiaochang block as an example. Based on the analysis of the production characteristics of the implemented wells, the production instability analysis method is used to analyze the variation rules and control factors of the single well EUR at different stages, and establish a single well EUR forecast analysis. plate. The analysis results show that the single-well EUR calculation result is more reliable for more than 2 years of continuous production, and can reach more than 80% of the final value. This method provides convenience for the rapid and accurate prediction of single-well EUR, and has a better field application effect.

In the actual production process of oil and gas reservoirs, the study of the redistribution characteristics of reservoir fluids is of great significance to the optimization of the indirect working system. Based on the nuclear magnetic resonance experiment, this paper analyzes the re-distribution of fluids in the core under the conditions of different shut-in times and different shut-in times. The distribution characteristics were studied. The results show that the water content increases with the increase of shut-in time and the number of shut-in times. For the same core, the water content in the large pores changes significantly.

From 2015 to 2019, a total of 7 wells in the Shan 28 well area of the Jingbian gas field used pumped bridge plug staged fracturing technology. The average test gas daily gas production rate is 8.13×104m3/d, and the average unblocked flow rate is 46.06×104m3/d, Demonstrating the superiority of the staged fracturing reconstruction process of pumping bridge plugs, However, due to the short time of using the pumped bridge plug staged fracturing process, the main controlling factors of fracturing reformation are not clear, resulting in a large gap between the gas production effect of the reformed well and the expected. Therefore, based on the site construction parameters, this paper uses single factor analysis and grey correlation analysis methods to determine the main control factors for fracturing reformation of the ancient sandstone horizontal well in the Jingbian gas field. Research shows that the length of gas layer, liquid type, average sand ratio, and sand volume are the main factors affecting the open flow and daily gas production of horizontal wells. The research results in this paper have certain guiding significance for the effective development of ancient sandstone horizontal wells.

At present, CNOOC has a large number of bottom water reservoirs developed by horizontal wells in the production oilfields, but the overall geological reserves of the oilfields are relatively low. The biggest problem facing this type of reservoir development and adjustment and tapping is the separation of the reservoir. The identification and quantitative characterization of the development characteristics of interlayer space. Therefore, in this paper, by formulating standards for the identification of barriers and intercalations, in-depth study of the profile and plane distribution characteristics of barriers and intercalations, and carrying out studies on the anatomy and characterization of the distribution of marine sandstone reservoirs. The results of the study show that the distribution of the study area on the profile is mainly composed of interlayers in the southwest and northwest of the legal person of the study area, with good connectivity and continuity. The distribution of interlayers on the plane is mainly muddy. Mainly with mezzanine, the research results in this paper are helpful to improve the accuracy of the anatomy of marine sandstone reservoir stacking relationship and the characterization accuracy of interlayer distribution, and improve the development effect of this type of reservoir.

After the completion of fracturing construction in unconventional oil and gas fields, the phenomenon of water hammer oscillation in the process of pump stopping during fracturing construction will lead to the inaccuracy of reflecting the trend of pressure transformation and increase the difficulty of fracturing effect evaluation in the process of fracturing construction. Therefore, three filtering methods, Savitzky-Golay filter, FFT filter and wavelet transform filter, are used to calibrate the measured pressure in this paper It is found that the method of wavelet transform can achieve the most ideal effect by comparing with the measured data. The field example of well X1 in Ordos Basin proves that the method of wavelet transform can accurately reflect the trend of pressure transformation. This method is of great significance to improve the evaluation of post pressure effect in the process of fracturing construction.