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36 results on '"Tonggu, Lige"'

5. Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization

Author

Shen, Min‐juan, primary, Jiao, Kai, additional, Wang, Chen‐yu, additional, Ehrlich, Hermann, additional, Wan, Mei‐chen, additional, Hao, Dong‐xiao, additional, Li, Jing, additional, Wan, Qian‐qian, additional, Tonggu, Lige, additional, Yan, Jian‐fei, additional, Wang, Kai‐yan, additional, Ma, Yu‐xuan, additional, Chen, Ji‐hua, additional, Tay, Franklin R., additional, and Niu, Li‐na, additional

Published
2022
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9. Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization

Author

Shen, Min‐juan, primary, Jiao, Kai, additional, Wang, Chen‐yu, additional, Ehrlich, Hermann, additional, Wan, Mei‐chen, additional, Hao, Dong‐xiao, additional, Li, Jing, additional, Wan, Qian‐qian, additional, Tonggu, Lige, additional, Yan, Jian‐fei, additional, Wang, Kai‐yan, additional, Ma, Yu‐xuan, additional, Chen, Ji‐hua, additional, Tay, Franklin R., additional, and Niu, Li‐na, additional

Published
2021
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14. Structure of the Cardiac Sodium Channel

Author

Jiang, Daohua, primary, Shi, Hui, additional, Tonggu, Lige, additional, Gamal El-Din, Tamer M., additional, Lenaeus, Michael J., additional, Zhao, Yan, additional, Yoshioka, Craig, additional, Zheng, Ning, additional, and Catterall, William A., additional

Published
2020
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19. Worming Their Way into Shape: Toroidal Formations in Micellar Solutions

Author

Cardiel, Joshua J., Tonggu, Lige, Dohnalkova, Alice C., de la Iglesia, Pablo, Pozzo, Danilo C., Wang, Liguo, and Shen, Amy Q.

Abstract

We report the formation of nanostructured toroidal micellar bundles (nTMB) from a semidilute wormlike micellar solution, evidenced by both cryogenic-electron microscopy and transmission electron microscopy images. Our strategy for creating nTMB involves a two-step protocol consisting of a simple prestraining process followed by flow through a microfluidic device containing an array of microposts, producing strain rates in the wormlike micelles on the order of 105s–1. In combination with microfluidic confinement, these unusually large strain rates allow for the formation of stable nTMB. Electron microscopy images reveal a variety of nTMB morphologies and provide the size distribution of the nTMB. Small-angle neutron scattering indicates the underlying microstructural transition from wormlike micelles to nTMB. We also show that other flow-induced approaches such as sonication can induce and control the emergence of onion-like and nTMB structures, which may provide a useful tool for nanotemplating.

Published
2024
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21. Collagen intrafibrillar mineralization as a result of the balance between osmotic equilibrium and electroneutrality

Author

Niu, Li-na, primary, Jee, Sang Eun, additional, Jiao, Kai, additional, Tonggu, Lige, additional, Li, Mo, additional, Wang, Liguo, additional, Yang, Yao-dong, additional, Bian, Ji-hong, additional, Breschi, Lorenzo, additional, Jang, Seung Soon, additional, Chen, Ji-hua, additional, Pashley, David H., additional, and Tay, Franklin R., additional

Published
2016
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34. Collagen intrafibrillar mineralization as a result of the balance between osmotic equilibrium and electroneutrality

Author

Niu, Li-na, Jee, Sang Eun, Jiao, Kai, Tonggu, Lige, Li, Mo, Wang, Liguo, Yang, Yao-dong, Bian, Ji-hong, Breschi, Lorenzo, Jang, Seung Soon, Chen, Ji-hua, Pashley, David H., and Tay, Franklin R.

Abstract

Mineralization of fibrillar collagen with biomimetic process-directing agents has enabled scientists to gain insight into the potential mechanisms involved in intrafibrillar mineralization. Here, by using polycation- and polyanion-directed intrafibrillar mineralization, we challenge the popular paradigm that electrostatic attraction is solely responsible for polyelectrolyte-directed intrafibrillar mineralization. As there is no difference when a polycationic or a polyanionic electrolyte is used to direct collagen mineralization, we argue that additional types of long-range non-electrostatic interaction are responsible for intrafibrillar mineralization. Molecular dynamics simulations of collagen structures in the presence of extrafibrillar polyelectrolytes show that the outward movement of ions and intrafibrillar water through the collagen surface occurs irrespective of the charges of polyelectrolytes, resulting in the experimentally verifiable contraction of the collagen structures. The need to balance electroneutrality and osmotic equilibrium simultaneously to establish Gibbs–Donnan equilibrium in a polyelectrolyte-directed mineralization system establishes a new model for collagen intrafibrillar mineralization that supplements existing collagen mineralization mechanisms.

Published
2017
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35. Collagen intrafibrillar mineralization as a result of the balance between osmotic equilibrium and electroneutrality

Author

Ji Hong Bian, Mo Li, Sang Eun Jee, Lorenzo Breschi, Franklin R. Tay, Li Na Niu, Ji Hua Chen, Yao Dong Yang, Lige Tonggu, Liguo Wang, Seung Soon Jang, Kai Jiao, David H. Pashley, Niu, Li-Na, Jee, Sang Eun, Jiao, Kai, Tonggu, Lige, Li, Mo, Wang, Liguo, Yang, Yao-Dong, Bian, Ji-Hong, Breschi, Lorenzo, Jang, Seung Soon, Chen, Ji-Hua, Pashley, David H., and Tay, Franklin R.

Subjects
0301 basic medicine, Fibrillar collagen, Fibrillar Collagens, Static Electricity, Condensed Matter Physic, 02 engineering and technology, Electrolyte, Molecular Dynamics Simulation, Article, 03 medical and health sciences, Electrolytes, Osmotic Pressure, Mechanics of Material, General Materials Science, Computer Simulation, Minerals, Chemistry, Mechanical Engineering, Chemistry (all), General Chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Polyelectrolyte, Collagen mineralization, 030104 developmental biology, Electrostatic attraction, Biochemistry, Mechanics of Materials, Biophysics, Materials Science (all), Intrafibrillar mineralization, 0210 nano-technology
Abstract

Mineralisation of fibrillar collagen with biomimetic process-directing agents has enabled scientists to gain insight into the potential mechanisms involved in intrafibrillar mineralisation. Here, by using polycation- and polyanion-directed intrafibrillar mineralisation, we challenge the popular paradigm that electrostatic attraction is solely responsible for polyelectrolyte-directed intrafibrillar mineralisation. Because there is no difference when a polycationic or a polyanionic electrolyte is used to direct collagen mineralisation, we argue that additional types of long-range non-electrostatic interactions are responsible for intrafibrillar mineralisation. Molecular dynamics simulations of collagen structures in the presence of extrafibrillar polyelectrolytes show that the outward movement of ions and intrafibrillar water through the collagen surface occurs irrespective of the charges of polyelectrolytes, resulting in the experimentally verifiable contraction of the collagen structures. The need to balance electroneutrality and osmotic equilibrium simultaneously to establish Gibbs-Donnan equilibrium in a polyelectrolyte-directed mineralisation system establishes a new model for collagen intrafibrillar mineralisation that supplements existing collagen mineralisation mechanisms.

Published
2015

36. Structural Basis for High-Affinity Trapping of the Na V 1.7 Channel in Its Resting State by Tarantula Toxin.

Author

Wisedchaisri G, Tonggu L, Gamal El-Din TM, McCord E, Zheng N, and Catterall WA

Subjects
Amino Acid Substitution, Animals, Humans, Mutation, Missense, NAV1.7 Voltage-Gated Sodium Channel genetics, NAV1.7 Voltage-Gated Sodium Channel metabolism, Sf9 Cells, Spodoptera, NAV1.7 Voltage-Gated Sodium Channel chemistry, Spider Venoms chemistry
Abstract

Voltage-gated sodium channels initiate electrical signals and are frequently targeted by deadly gating-modifier neurotoxins, including tarantula toxins, which trap the voltage sensor in its resting state. The structural basis for tarantula-toxin action remains elusive because of the difficulty of capturing the functionally relevant form of the toxin-channel complex. Here, we engineered the model sodium channel Na V Ab with voltage-shifting mutations and the toxin-binding site of human Na V 1.7, an attractive pain target. This mutant chimera enabled us to determine the cryoelectron microscopy (cryo-EM) structure of the channel functionally arrested by tarantula toxin. Our structure reveals a high-affinity resting-state-specific toxin-channel interaction between a key lysine residue that serves as a "stinger" and penetrates a triad of carboxyl groups in the S3-S4 linker of the voltage sensor. By unveiling this high-affinity binding mode, our studies establish a high-resolution channel-docking and resting-state locking mechanism for huwentoxin-IV and provide guidance for developing future resting-state-targeted analgesic drugs., Competing Interests: Declaration of Interests The authors declare no competing interests., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Published
2021
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