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Your search keyword '"Tong, Jianhao"' showing total 33 results
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33 results on '"Tong, Jianhao"'

20. Cr(VI) Reduction and Fe(II) Regeneration by Penicillium oxalicumSL2-Enhanced Nanoscale Zero-Valent Iron

Author

Luo, Yating, Pang, Jingli, Peng, Cheng, Ye, Jien, Long, Bibo, Tong, Jianhao, and Shi, Jiyan

Abstract

Nanoscale zero-valent iron (nZVI) faces significant challenges in Cr(VI) remediation through aggregation and passivation. This study identified a Cr(VI)-resistant filamentous fungus (Penicillium oxalicumSL2) for nZVI activation and elucidated the synergistic mechanism in chromium remediation. P. oxalicumSL2 and nZVI synergistically and effectively removed Cr(VI), mainly by extracellular nonenzymatic reduction (89.1%). P. oxalicumSL2 exhibited marked iron precipitate solubilization and Fe(II) regeneration capabilities. The existence of the Fe(II)–Cr(V)-oxalate complex (HCrFeC4O9) indicated that in addition to directly reducing Cr(VI), iron ions generated by nZVI stimulated Cr(VI) reduction by organic acids secreted by P. oxalicumSL2. RNA sequencing and bioinformatics analysis revealed that P. oxalicumSL2 inhibited phosphate transport channels to suppress Cr(VI) transport, facilitated iron and siderophore transport to store Fe, activated the glyoxylate cycle to survive harsh environments, and enhanced organic acid and riboflavin secretion to reduce Cr(VI). Cr(VI) exposure also stimulated the antioxidative system, promoting catalase activity and maintaining the intracellular thiol/disulfide balance. Cr(VI)/Fe(III) reductases played crucial roles in the intracellular reduction of chromium and iron, while nZVI decreased cellular oxidative stress and alleviated Cr(VI) toxicity to P. oxalicumSL2. Overall, the P. oxalicumSL2–nZVI synergistic system is a promising approach for regenerating Fe(II) while reducing Cr(VI).

Published
2023
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31. Dual Regulatory Role of Penicillium oxalicumSL2 in Soil: Phosphorus Solubilization and Pb Stabilization

Author

Tong, Jianhao, Wu, Hanxin, Jiang, Xiaohan, Ruan, Chendao, Li, Weilong, Zhang, Haonan, Pan, Siyi, Wang, Jing, Ren, Jiayu, Zhang, Chun, and Shi, Jiyan

Abstract

The mechanisms of the P. oxalicumSL2-mediated microbial community on phosphorus solubilization and Pb stabilization were investigated through a 90-day soil experiment. In the treatments inoculated with P. oxalicumSL2, the amount of P. oxalicumSL2-GFP remained at 77.8%–138.6% of the initial inoculation amount after 90 days, and the available phosphorus (AP) content increased 21.7%–40.8% while EDTA-Pb decreased 29.9%–43.2% compared with CK treatment. SEM-EDS results showed that P. oxalicumSL2 changed the agglomeration degree of microaggregates and promoted the combination of Pb with C and O elements. These phenomena were enhanced when applied with Ca3(PO4)2. Microbial community analysis showed that P. oxalicumSL2 improved soil microbial activity, in which the fungi absolute abundance increased about 15 times within 90 days. Correlation analyses and a partial least-squares path model showed that the activation of Penicillium, Ascobolus, Humicola, and Spizellomycesin a fungal community increased the content of oxalate and AP, which directly decreased EDTA-Pb content, while the change of Bacillus, Ramlibacter, Gemmatimonas, and Candidatus Solibacterin the bacterial community regulated Fe/Mn/S/N cycle-related functions, thus promoting the conversion of Pb to oxidizable state. Our findings highlight that P. oxalicumSL2 enhanced the microbial-induced phosphate precipitation process by activating soil microbial communities and regulating their ecological functions.

Published
2023
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33. Dual Regulatory Role of Penicillium oxalicum SL2 in Soil: Phosphorus Solubilization and Pb Stabilization.

Author

Tong J, Wu H, Jiang X, Ruan C, Li W, Zhang H, Pan S, Wang J, Ren J, Zhang C, and Shi J

Subjects
Lead, Soil, Edetic Acid, Phosphorus, Penicillium
Abstract

The mechanisms of the P. oxalicum SL2-mediated microbial community on phosphorus solubilization and Pb stabilization were investigated through a 90-day soil experiment. In the treatments inoculated with P. oxalicum SL2, the amount of P. oxalicum SL2-GFP remained at 77.8%-138.6% of the initial inoculation amount after 90 days, and the available phosphorus (AP) content increased 21.7%-40.8% while EDTA-Pb decreased 29.9%-43.2% compared with CK treatment. SEM-EDS results showed that P. oxalicum SL2 changed the agglomeration degree of microaggregates and promoted the combination of Pb with C and O elements. These phenomena were enhanced when applied with Ca 3 (PO 4 ) 2 . Microbial community analysis showed that P. oxalicum SL2 improved soil microbial activity, in which the fungi absolute abundance increased about 15 times within 90 days. Correlation analyses and a partial least-squares path model showed that the activation of Penicillium , Ascobolus , Humicola , and Spizellomyces in a fungal community increased the content of oxalate and AP, which directly decreased EDTA-Pb content, while the change of Bacillus , Ramlibacter , Gemmatimonas , and Candidatus Solibacter in the bacterial community regulated Fe/Mn/S/N cycle-related functions, thus promoting the conversion of Pb to oxidizable state. Our findings highlight that P. oxalicum SL2 enhanced the microbial-induced phosphate precipitation process by activating soil microbial communities and regulating their ecological functions.

Published
2024
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