4 results on '"Tommaso Petochi"'
Search Results
2. Effects of Dietary Plant Protein Replacement with Insect and Poultry By-Product Meals on the Liver Health and Serum Metabolites of Sea Bream (Sparus aurata) and Sea Bass (Dicentrarchus labrax)
- Author
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Valeria Donadelli, Patrizia Di Marco, Alberta Mandich, Maria Grazia Finoia, Gloriana Cardinaletti, Tommaso Petochi, Alessandro Longobardi, Emilio Tibaldi, and Giovanna Marino
- Subjects
liver histology ,liver index ,sustainable feed ,poultry by-product meal ,Hermetia illucens meal ,plant protein ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
The liver health of Gilthead sea bream and European sea bass, fed with fish meal-free diets, including various proportions of plant proteins, as well as insect and poultry by-product meals, was investigated through biochemical and histological analyses using a new liver index (LI) formula. Four isoproteic (45% Dry Matter, DM) and isolipidic (20% DM) diets were compared, including a plant-based control diet (CV) and three other test diets, in which 40% of a plant protein-rich ingredient mixture was replaced with meals from Hermetia illucens (H40) or poultry by-product (P40) alone, or in combination (H10P30). The trials lasted 12 and 18 weeks for sea bream and sea bass, respectively. The results obtained thus far highlighted species-specific differences in the physiological response to dietary changes. In sea bream, the biochemical and histological responses suggest favorable physiological and liver health statuses, with higher serum cholesterol (CHO) and triglyceride (TAG) levels, as well as moderate hepatocyte lipid accumulation, with the H10P30 diet compared to the CV (p < 0.05). In sea bass, all diets resulted in elevated serum TAG levels and lipid accumulation in the liver, particularly in fish fed the P40 one (p < 0.05), which resulted in the highest LI, coupled with a higher frequency of severe lipid accumulation, hypertrophy, cord loss, peripheral nuclei displacement, and pyknosis. In conclusion, sea bream adapted well to the test diets, whereas sea bass exhibited altered hepatic lipid metabolism leading to incipient liver steatosis, likely due to the high lipid contents of the diets, including the insect and poultry meals. The LI formula developed in this study proved to be a reliable tool for assessing the effects of dietary changes on the liver health of sea bream and sea bass, consistent with biochemical and histological findings.
- Published
- 2024
- Full Text
- View/download PDF
3. Growth and Welfare of Rainbow Trout (Oncorhynchus mykiss) in Response to Graded Levels of Insect and Poultry By-Product Meals in Fishmeal-Free Diets
- Author
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Gloriana Cardinaletti, Patrizia Di Marco, Enrico Daniso, Maria Messina, Valeria Donadelli, Maria Grazia Finoia, Tommaso Petochi, Francesca Fava, Filippo Faccenda, Michela Contò, Roberto Cerri, Donatella Volpatti, Chiara Bulfon, Alberta Mandich, Alessandro Longobardi, Giovanna Marino, Lina Fernanda Pulido-Rodriguez, Giuliana Parisi, and Emilio Tibaldi
- Subjects
alternative proteins ,digestive function ,immune response ,Hermetia illucens ,nutrient retention ,poultry by-product meal ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
This study compared the nutrient-energy retention, digestive function, growth performance, and welfare of rainbow trout (ibw 54 g) fed isoproteic (42%), isolipidic (24%), fishmeal-free diets (CV) over 13 weeks. The diets consisted of plant-protein replacement with graded levels (10, 30, 60%) of protein from poultry by-product (PBM) and black soldier fly H. illucens pupae (BSFM) meals, either singly or in combination. A fishmeal-based diet was also tested (CF). Nitrogen retention improved with moderate or high levels of dietary PBM and BSFM relative to CV (p < 0.05). Gut brush border enzyme activity was poorly affected by the diets. Gastric chitinase was up-regulated after high BSFM feeding (p < 0.05). The gut peptide and amino acid transport genes were differently regulated by protein source and level. Serum cortisol was unaffected, and the changes in metabolites stayed within the physiological range. High PBM and high BSFM lowered the leukocyte respiratory burst activity and increased the lysozyme activity compared to CV (p < 0.05). The BSFM and PBM both significantly changed the relative percentage of lymphocytes and monocytes (p < 0.05). In conclusion, moderate to high PBM and BSFM inclusions in fishmeal-free diets, either singly or in combination, improved gut function and nutrient retention, resulting in better growth performance and the good welfare of the rainbow trout.
- Published
- 2022
- Full Text
- View/download PDF
4. Effects of dietary plant proteins on immune system in turbot juveniles Psetta maxima
- Author
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Tommaso Petochi, Valeria Donadelli, Patrizia Di Marco, Alessandra Priori, Maria Grazia Finoia, Alessandro Longobardi, Ramon Fontanillas, Giovanna Marino, GATTA, PIER PAOLO, BONALDO, ALESSIO, PARMA, LUCA, Tommaso Petochi, Valeria Donadelli, Patrizia Di Marco, Alessandra Priori, Maria Grazia Finoia, Alessandro Longobardi, Pier Paolo Gatta, Alessio Bonaldo, Luca Parma, Ramon Fontanilla, and Giovanna Marino
- Subjects
GROWTH ,TURBOT ,WELFARE - Abstract
The impact of fish meal replacement with plant proteins on fish health is still poorly investigated. This study evaluates the effects of several diets containing graded levels of a mixture of gluten meal, soybean meal and soy protein concentrate on some immune parameters of turbot juveniles. The feeding trial was carried out at the aquaculture facilities of the Veterinary University of Bologna (Cesenatico, Italy). Turbot juveniles (9.7kept at 18in 500 l recirculating tanks (in triplicate) were fed four isonitrogenous (51%) and isolipidic diets (16%) (Skretting ARC, Stavanger, NO) for 9 weeks: a reference diet of 50% fish meal (FM50), and three diets containing 35%, 20% and 5% fish meal (FM35; FM20; FM5), with a proportional amount of vegetable ingredients. At the end of the trial, blood samples and spleen tissues were collected and analyzed for serum lysozyme concentration (lysoplate assay), differential leukocyte count (blood smears/May-Grunwald Giemsa) and quantification of splenic melano-macrophage centres (MMCs) (histology/image analyzer). One-way ANOVA and c2-test were applied respectively to humoral and cellular immune parameters to test the effects of dietary treatment. Plant protein diets induced significant changes in several immune parameters in turbot juveniles. Serum lysozyme was higher in turbot fed on FM35 and FM20 diets. The percentage of circulating phagocytes, mainly neutrophils, and splenic MMCs, increased, especially in fish fed FM5 and FM20 diets. No difference was observed in lymphocyte population. The increase in blood phagocytes could be responsible for the rise in serum lysozyme and interpretable as a stress or inflammatory response. Conversely, the lower lysozyme level measured in turbot fed FM5 diet in the face of a higher number of circulating phagocytes could be interpreted as a functional impairment of these cells. The increase in MMCs may be associated with a nutritional deficit, which is more severe in fish fed FM5. Long-term feeding trials are needed in order to gain further insight into the impact of dietary plant proteins on immune function and health in turbot.
- Published
- 2012
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