20 results on '"Tomičić, Ružica"'
Search Results
2. Dried Beetroots: Optimization of the Osmotic Dehydration Process and Storage Stability
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Šuput, Danijela, primary, Rakita, Slađana, additional, Spasevski, Nedeljka, additional, Tomičić, Ružica, additional, Dragojlović, Danka, additional, Popović, Senka, additional, and Hromiš, Nevena, additional
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- 2024
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3. Diversity of amino acids composition in cereals
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Tomičić Zorica M., Pezo Lato L., Spasevski Nedeljka J., Lazarević Jasmina M., Čabarkapa Ivana S., and Tomičić Ružica M.
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essential amino acids ,non-essential amino acids ,chemical composition ,nutritional profiling ,dietary reference intakes ,Food processing and manufacture ,TP368-456 - Abstract
The quality of protein is based on their amino acid composition, especially on the content and availability of essential amino acids. Cereals are important sources of protein for human nutrition, but are limited in the amounts of essential amino acids, notably lysine. The aim of this study was to analyze the chemical composition and amino acid profiles of different cereals that are important for nutritional purposes in human diet. The content of protein, moisture and crude fat in cereals varied significantly from 7.83 to 13.22%, 11.45 to 13.80%, and from 1.67 to 6.35%, respectively. The obtained results showed that oat had the highest contents of crude protein (13.22%), crude fat (6.35%) and crude cellulose (9.42%) compared to other cereals. Significant (p < 0.05) variation existed in the content of essential and nonessential amino acids among samples with the highest level in oat and wheat. Essential amino acids accounted for one-third of the total amino acids in the tested cereals. Glutamic acid was found to be the most abundant amino acid. It could be concluded that the amino acid composition of oat is the most favorable among cereals due to its high protein content and the content of lysine which can be found in limited amounts in most of the cereals.
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- 2022
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4. Essential oils as antimicrobial and anti-adhesion agents against bacteria Salmonella Typhimurium and Staphylococcus aureus, and yeasts Candida albicans and Saccharomyces cerevisiae
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Tomičić Zorica M., Tomičić Ružica M., Kocić-Tanackov Sunčica D., and Raspor Peter
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essential oils ,antimicrobial agents ,anti-adhesion agents ,bacteria ,yeasts ,Food processing and manufacture ,TP368-456 - Abstract
A serious global problem with the increasing resistance of microorganisms to currently used antimicrobials has opened up the promotional research in the identification of new, more effective drugs with a broad spectrum of antimicrobial activities. Plant essential oils, due to the large biological and structural diversity of their components, are known to have many potential benefits. This study aimed to evaluate the antimicrobial and anti-adhesion activity of fifteen essential oils and their compounds against two bacterial and two yeast species responsible for food spoilage and infectious diseases. Antimicrobial activity was determined by testing the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC) and the minimum fungicidal concentration (MFC) of essential oils and compounds. The essential oils of Cinnamomum zeylanicum and Eugenia caryophyllus showed the highest antimicrobial activity with MICs ranging from 0.078 to 1.25 mg/mL, and 0.039 to 1.25 mg/mL, respectively. On the other hand, essential oils of Eucalypti aetheroleum and Salvia officinalis had significantly weaker antimicrobial properties than the others. Further, MICs were used to assess the inhibition of adhesion of bacteria Salmonella Typhimurium ATCC 25923 and Staphylococcus aureus ATCC 14208, and yeasts Candida albicans ATCC 10231 and Saccharomyces cerevisiae ATCC 9763 in a microtiter plate using the crystal violet staining method. Based on the percentage of adhesion inhibition, yeast S. cerevisiae ATCC 9763 showed a high level of antimicrobial resistance. E. caryophyllus had the strongest effect with inhibition up to 73%. Consistent with the antimicrobial susceptibility results, the most active anti-adhesion compounds were carvacrol and thymol. Considering the role of biofilm in food spoilage and clinical diseases, inhibition of the initial phase of biofilm formation by natural antimicrobial agents may be an alternative to commonly used synthetic ones.
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- 2022
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5. Food grade disinfectants based on hydrogen peroxide/peracetic acid and sodium hypochlorite interfere with the adhesion of Escherichia coli , Pseudomonas aeruginosa , Staphylococcus aureus and Listeria monocytogenes to stainless steel of differing surface roughness
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Tomičić, Ružica, primary, Tomičić, Zorica, additional, Nićetin, Milica, additional, Knežević, Violeta, additional, Kocić-Tanackov, Sunčica, additional, and Raspor, Peter, additional
- Published
- 2023
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6. Factors influencing adhesion of bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and yeast Pichia membranifaciens to wooden surfaces
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Tomičić, Ružica, Tomičić, Zorica, Thaler, Nejc, Humar, Miha, and Raspor, Peter
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- 2020
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7. By-products of the oil industry as sources of amino acids in feed
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Tomičić Zorica M., Spasevski Nedeljka J., Popović Sanja J., Banjac Vojislav V., Đuragić Olivera M., and Tomičić Ružica M.
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amino acids ,soybean meal ,soybean cake ,sunflower meal ,Food processing and manufacture ,TP368-456 - Abstract
A global increase in the demand for livestock products suggests that there will be a consequent rise in demand for feed, not only of cereals but of other feeds and particularly proteins. In the present study, oil industry by-products such as soybean meal, soybean cake and sunflower meal were analysed as sources of amino acids in animal nutrition. From among oilseed byproducts, the soybean meal content the most of crude protein up to 44% and the best of amino acid composition, while content of crude cellulose (about 6%) is lower in comparison to other oilseed meals. The results showed that the total amino acids in the examined samples ranged from 31.87 to 41.01%, and the total essential and nonessential amino acids varied from 13.41 to 17.38% and from 18.46 to 23.76%, respectively. Generally, the protein contained in soybean meal and cake was rich in essential amino acids. However, because of the lowest amino acid score, methionine was considered as a limiting amino acid in both soybean by-products. On the other hand, soya's meal contained higher level of lysine than other protein-based vegetable alternative to soya like sunflower meals examined in this study. Glutamic acid, aspartic acid, leucine and valine were the most abundant amino acids in all tested by-products of the oil industry. Therefore, partial substitution of protein sources in feeds with proteins from the oil industry by-products may improve feed quality.
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- 2020
8. Camelina sativa Oilseed Cake as a Potential Source of Biopolymer Films: A Chemometric Approach to Synthesis, Characterization, and Optimization.
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Šuput, Danijela, Pezo, Lato, Rakita, Slađana, Spasevski, Nedeljka, Tomičić, Ružica, Hromiš, Nevena, and Popović, Senka
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CAMELINA ,CAKE ,CHEMOMETRICS ,WATER vapor ,COMPOSITE structures ,BIOPOLYMERS - Abstract
In this work, the possibility of obtaining biopolymer films from Camelina sativa oilseed cake (CSoC) at different parameters of the synthesis process was investigated. The pH (values 8, 10, and 12), the temperature (60, 80, and 100 °C), and the concentration of the cake in the film-forming suspension (3, 4, and 5%) were varied. The films obtained were characterized by studying the mechanical, barrier, physico-chemical, structural, and biological properties (antioxidant and antimicrobial). The results showed moderate mechanical properties, indicating the application of these biopolymer materials as coatings. Low values of water vapor permeability (5.1–12.26 g/m
2 h) and very low transmission in the UV range (less than 2%) indicate good barrier properties. FTIR analysis confirmed the films' composite structures: peaks related to proteins, polysaccharides, lipids, and cellulose were detected. The biological activity of the obtained CSoC films was pronounced so that they could be used for active packaging. All films have high antioxidant activity, which is more pronounced in samples synthesized at pH 8. The films possess antibacterial properties against S. aureus, while some had an inhibitory effect on S. typhimurium. Standard score analysis showed that the film sample synthetized at pH = 10, temperature = 100 °C, and concentration = 5% had optimal investigated properties. [ABSTRACT FROM AUTHOR]- Published
- 2024
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9. Antimicrobial activity of essential oils against Listeria monocytogenes
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Tomičić Ružica M., Čabarkapa Ivana S., Varga Ana O., and Tomičić Zorica M.
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Listeria monocytogenes ,antimicrobial activity ,essential oils ,antibiotics ,broth microdilution method ,MIC Test Strip ,Food processing and manufacture ,TP368-456 - Abstract
Food poisoning caused by Listeria monocytogenes leads to a 30% rate of mortality among patients. The application of essential oils (EOs) to food products is a suitable strategy to control pathogens and to extend their shelf life by reducing microbial levels. The objective of this study was to evaluate the antimicrobial potential of essential oils (EOs) against L. monocytogenes. The EOs used in this study were caraway (Carum carvi), cinnamon (Cinnamomum zeylanicum), dill (Anethum graveolens), clove (Syzygium aromaticum), mentha (Menthae piperitae aetheroleum), red thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), common sage (Salvia officinalis), clary sage (Salvia sclarea) and summer savory (Satureja hortensis). The minimal inhibitory concentrations (MICs) of EOs were determined using the broth microdilution method. According to the MIC values, all essential oils were effective in the inhibition of L. monocytogenes strains, with MICs varying from 256 μg/ml to 4096 μg/ml. The results showed that cinnamon EO had the highest antimicrobial activity, while dill and mentha EOs were the least effective against the L. monocytogenes. In addition, two different procedures were carried out to test the effect of antibiotics gentamycin and streptomycin against the L. monocytogenes strains, the broth microdilution method and the MIC Test Strip. Our results indicated that the reference strain L. monocytogenes ATCC 19111 was much more sensitive to antibiotics than L. monocytogenes strains isolated from meat, highlighting that gentamycin was the more effective in comparison to streptomycin.
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- 2018
10. Survivability of Salmonella Enteritidis enclosed into biofilm
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Čabarkapa Ivana, Vukmirović Đuro, Milanov Dubravka, Kokić Bojana, Spasevski Nedeljka, Tomičić Ružica, and Rakita Slađana
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salmonella enteritidis ,biofilm ,surviving ,feed ,Agriculture - Abstract
It is known that some pathogens in feed production plants may exist throughout several months, even years. These strains are known as 'house strains' and the assumption is that the existence of such strains is enabled due to their ability to form biofilms. The objective of our experiment was to assess survivability of selected isolates that were previously characterized as rdar morphotype enclosed into biofilm on glass surfaces. Testing of cell viability in the biofilms was carried out in the condition of desiccation (28 days) and with periodic availability of nutrients (90 days) at ambient temperature. In both of tested conditions percent of viable cells was more than 50 % for all tested isolates. The obtained results suggest that survivability of Salmonella Enteritidis rdar morphotype cells enclosed into biofilm on glass surfaces under conditions of desiccation and periodic availability of nutrients may present a serious problem in the food and feed industry due to biofilm production, which contributes to their spreading throughout the whole facility and consequently increasing cross-contamination risks.
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- 2016
11. Influence of growth conditions on biofilm formation of Listeria monocytogenes
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Tomičić Ružica M., Čabarkapa Ivana S., Vukmirović Đuro M., Lević Jovanka D., and Tomičić Zorica M.
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biofilm formation ,Listeria monocytogenes ,growth medium ,temperature ,Food processing and manufacture ,TP368-456 - Abstract
Listeria monocytogenes is ubiquitous in nature and a major concern for the food industry, since it is the causal agent of the serious foodborne illness listeriosis. This organism can be introduced through many routes to food-processing environments and may become established on food-processing equipment. Subsequently, food products may become contaminated during processing. In addition, the bacterium can grow at refrigeration temperatures. Biofilms are regarded as important with respect to the survival and growth of microorganisms in the food industry. Microorganisms growing in biofilms are protected against cleaning and disinfection and are difficult to eradicate. L. monocytogenes may grow in biofilms that protect them against environmental stress and can be isolated from surfaces after cleaning and disinfection. In this study, a total of eight L. monocytogenes strains isolated from the meat industry and one reference strain L. monocytogenes ATCC 19111 were studied for their capability to form a biofilm. The biofilm forming behavior of nine L. monocytogenes strains was determined in two different media, Tryptone soya yeast extract broth (TSYEB) or Brain-heart infusion broth (BHI), at temperatures 7 °C, 25 °C, 37 °C, 42 °C for 5 days. The method used to assess biofilm formation was crystal violet staining. All strains were able to form biofilm, but the growth condition affected the levels formed. The lowest biofilm formation was observed at 7 °C. Further, the most effective medium in promoting biofilm production by the L. monocytogenes isolates from meat was BHI medium while for reference strain L. monocytogenes ATCC 19111 it was TSYEB. Incubation temperature was the most significant factor influencing the biofilm production levels, and also the type of used nutritive medium was important factor.
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- 2016
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12. Beneficial properties of probiotic yeast Saccharomyces boulardii
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Tomičić Zorica M., Čolović Radmilo R., Čabarkapa Ivana S., Vukmirović Đuro M., Đuragić Olivera M., and Tomičić Ružica M.
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Saccharomyces boulardii ,efficacy ,safety ,probiotic ,Food processing and manufacture ,TP368-456 - Abstract
Saccharomyces boulardii is unique probiotic and biotherapeutic yeast, known to survive in gastric acidity and it is not adversely affected or inhibited by antibiotics or does not alter or adversely affect the normal microbiota. S. boulardii has been utilized worldwide as a probiotic supplement to support gastrointestinal health. The multiple mechanisms of action of S. boulardii and its properties may explain its efficacy and beneficial effects in acute and chronic gastrointestinal diseases that have been confirmed by clinical trials. Caution should be taken in patients with risk factors for adverse events. Its potential application in various dairy foods could offer an alternative probiotic product to people suffering from antibiotic-associated diarrhea. This review discusses the evidence for efficacy and safety of S. boulardii as a probiotic for the prevention and therapy of gastrointestinal disorders in humans.
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- 2016
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13. Identification and Characterization of 33 Bacillus cereus sensu lato Isolates from Agricultural Fields from Eleven Widely Distributed Countries by Whole Genome Sequencing
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Aggerbeck, Marie Rønne, Zghal, Raida Zribi, Hendriksen, Niels Bohse, Zervas, Athanasios, Aggerbeck, Marie, Allaga, Henrietta, Güzel, Mustafa, Hendriks, Marc, Jonuškienė, IIona, Kedves, Orsolya, Kupeli, Ayse, Lamovšek, Janja, Mülner, Pascal, Munday, Denise, Namli, Şahin, Samut, Hilal, Tomičić, Ružica, Tomičić, Zorica, Yeni, Filiz, Zghal, Raida, Zhao, Xingchen, Sanchis-Borja, Vincent, Hendriksen, Niels, Aarhus University [Aarhus], Université de Sfax - University of Sfax, University of Szeged [Szeged], Hitit University, Wageningen University and Research [Wageningen] (WUR), Kaunas University of Technology (KTU), Middle East Technical University [Ankara] (METU), Agricultural Institute of Slovenia, Agricultural Institute, Graz University of Technology [Graz] (TU Graz), Sumitomo Chemical Agro Europe, Partenaires INRAE, University of Novi Sad, Centre de Biotechnologie de Sfax (CBS), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), European Cooperation in Science and Technology's COST-action 16110, [Belirlenecek], and MDPI AG (Basel, Switzerland)
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Microbiology (medical) ,Ecotypes ,Technology and Engineering ,[SDV]Life Sciences [q-bio] ,Bacillus toyonensis ,DIVERSITY ,ecotypes ,Bacillus thuringiensis ,AFFILIATION ,Bacillus mosaicus ,Bacillus ,Context (language use) ,mycoides ,GROUP STRAINS ,phylogeny ,Microbiology ,Article ,03 medical and health sciences ,Biointeractions and Plant Health ,Sensu ,geographical-distribution ,Phylogenetics ,Virology ,parasitic diseases ,lcsh:QH301-705.5 ,Phylogeny ,030304 developmental biology ,Bacillus mycoides ,Genetics ,Whole genome sequencing ,patho-types ,0303 health sciences ,biology ,Phylogenetic tree ,Ecotype ,030306 microbiology ,fungi ,Geographical-distribution ,biology.organism_classification ,bacterial infections and mycoses ,Cereus ,lcsh:Biology (General) ,WEIHENSTEPHANENSIS ,Identification (biology) ,Patho-types ,geographic locations - Abstract
The phylogeny, identification, and characterization of 33 B. cereus sensu lato isolates originating from 17 agricultural soils from 11 countries were analyzed on the basis of whole genome sequencing. Phylogenetic analyses revealed all isolates are divided into six groups, which follows the generally accepted phylogenetic division of B. cereus sensu lato isolates. Four different identification methods resulted in a variation in the identity of the isolates, as none of the isolates were identified as the same species by all four methods&mdash, only the recent identification method proposed directly reflected the phylogeny of the isolates. This points to the importance of describing the basis and method used for the identification. The presence and percent identity of the protein product of 19 genes potentially involved in pathogenicity divided the 33 isolates into groups corresponding to phylogenetic division of the isolates. This suggests that different pathotypes exist and that it is possible to differentiate between them by comparing the percent identity of proteins potentially involved in pathogenicity. This also reveals that a basic link between phylogeny and pathogenicity is likely to exist. The geographical distribution of the isolates is not random: they are distributed in relation to their division into the six phylogenetic groups, which again relates to different ecotypes with different temperature growth ranges. This means that we find it easier to analyze and understand the results obtained from the 33 B. cereus sensu lato isolates in a phylogenetic, patho-type and ecotype-oriented context, than in a context based on uncertain identification at the species level.
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- 2020
14. Identification and Characterization of 33 Bacillus cereus sensu lato Isolates from Agricultural Fields from Eleven Widely Distributed Countries by Whole Genome Sequencing
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Zervas, Athanasios, primary, Aggerbeck, Marie Rønne, additional, Allaga, Henrietta, additional, Güzel, Mustafa, additional, Hendriks, Marc, additional, Jonuškienė, IIona, additional, Kedves, Orsolya, additional, Kupeli, Ayse, additional, Lamovšek, Janja, additional, Mülner, Pascal, additional, Munday, Denise, additional, Namli, Şahin, additional, Samut, Hilal, additional, Tomičić, Ružica, additional, Tomičić, Zorica, additional, Yeni, Filiz, additional, Zghal, Raida Zribi, additional, Zhao, Xingchen, additional, Sanchis-Borja, Vincent, additional, and Hendriksen, Niels Bohse, additional
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- 2020
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15. Antifungal and anti-adhesion activity of plant extracts and essential oils against Candida spp. and Pichia spp.
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TOMIČIĆ, ZORICA, TOMIČIĆ, RUŽICA, SMOLE MOŽINA, SONJA, BUCAR, FRANZ, TUREK, IVANA, and RASPOR, PETER
- Subjects
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ESSENTIAL oils , *PLANT extracts , *ANTIFUNGAL agents , *PICHIA , *ROSEROOT , *CANDIDA , *THYMES , *CANDIDA albicans - Abstract
The use of natural antifungal agents has gained much attention to extend shelf-life, increase the safety of food products in the food industry and inhibit disease-causing microorganisms. The objective of this study was to evaluate the antifungal and anti-adhesion potential of 15 plant extract, essential oils and chemical compounds against Candida spp. and Pichia spp. Susceptibility tests indicated that essential oils of Cinnamomum verum and Origanum vulgare had the highest inhibitory effect. For most extracts and essential oils examined, Pichia membranifaciens ZIM 2417 was the most sensitive yeast, while Candida glabrata ZIM 2369 proved to be the least sensitive one. Anti-adhesion ability of the plant extracts and essential oils against C. glabrata ZIM 2369 was estimated by the standard crystal violet assay. The results showed that the essential oils of C. verum, O.vulgare, Satureja montana and Thymus vulgaris possessed promising activity against the initial phase of biofilm formation and the pre-formed 24 h biofilm of C. glabrata. On the other hand, Sedum roseum extract showed the strongest anti-adhesion effect. Results of this study support the use of plant extracts, essential oils and their biologically active components against C. glabrata. [ABSTRACT FROM AUTHOR]
- Published
- 2022
16. Uticaj faktora sredine na adheziju kvasaca na abiotičke površine
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Tomičić, Ružica, Dodić, Siniša, Raspor, Peter, Grahovac, Jovana, and Savić, Dragiša
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Adhezija ,kvasci ,Adhesion ,yeasts ,Adhesion, yeasts, abiotic surfaces ,abiotičke površine ,Adhezija, kvasci, abiotičke površine ,abiotic surfaces - Abstract
Adhezija i formiranje biofilma kvasaca na kontaktne površine materijala je ozbiljan higijenski problem u prehrambenoj industriji, jer može biti izvor kontaminacije tokom proizvodnje, dovodeći do velikih ekonomskih gubitaka zbog kvarenja hrane i smanjenja kvaliteta proizvoda, a takođe može da šteti zdravlju potrošača. S drge strane, mnoge infekcije u bolnici uzrokovane su adhezijom vrsta roda Candida na medicinska sredstva i implantate. Međutim, trenutno razumevanje adhezije i kolonizacije kvasaca na abiotičke površine je ograničeno na samo neke vrste roda Candida, a naročito malo se zna o vrstama roda Pichia kao adhezivnim agensima.Cilj istraživanja u okviru ove doktorske disertacije je bio da se proceni potencijal adhezije sojeva kvasaca Candida i Pichia na polistiren površinu, diskove od nerđajućeg čelika (AISI 304, Ra = 25.20 – 961.9 nm) i drveta sa različitim stepenom hrapavosti površine, i ispita uticaj hrapavosti površine na adheziju kvasaca. Hidrofobnost površine ćelije (CSH) sojeva iz rodova Candida i Pichia je određena u cilju procene korelacije između hidrofobnosti površine ćelije i adhezije kvasaca na polistiren. Pored toga, ispitan je uticaj različitih medijuma, temperatura, i različitih biljnih ekstrakata na adheziju odabranih sojeva iz rodova Candida i Pichia na površine diskova od nerđajućeg čelika. Rezultati su pokazali da su svi testirani sojevi kvasaca Candida i Pichia su bili u stanju da adheriraju na polistiren, površine od nerđajućeg čelika i drveta, ali u različitoj meri. Soj vrste C. albicans je imao veću sposobnost adhezije na polistiren i nerđajući čelik u odnosu na sojeve vrsta C. glabrata, C. parapsilosis i C. krusei. U slučaju kod roda Pichia, sojevi vrste P. membranifaciens su pokazali manju sposobnost adhezije na nerđajući čelik nego ispitivani soj vrste P. pijperi, što nije slučaj kod adhezije na polistiren. Na osnovu dobijenih rezultata pokazano je da su sojevi vrsta C. glabrata, C. parapsilosis, C. krusei, P. pijperi i P. membranifaciens bili izrazito do umereno hidrofobni, dok je soj vrste C. albicans bio hidrofilan, što ukazuje da hidrofobnost površine ćelije (CSH) nije značajno u korelaciji sa količinom ćelija adheriranih na polistiren. Pored toga, na adheziju sojeva kvasaca Candida i Pichia značajno je uticala hrapavost površine nerđajućeg čelika, dok hrapavost drvenih površina nije značajno uticala. Dalje mi smo ispitali uticaj različitih medijuma za rast i temperature na adheziju odabranih kvasaca. Primećeno je da je količina adheriranih ćelija kvasaca C. albicans i C. glabrata na površinama od nerđajućeg čelika bila znatno veća u Malt Extract Broth (MEB) medijumu, dok je za C. parapsilosis, C. krusei, P. pijperi i P. membranifaciens bila u Yeast Peptone Dextrose (YPD) medijumu. Naši podaci takođe ukazuju da je temperatura veoma važan faktor koji značajno utiče na adheziju ovih kvasaca. Problem adhezije i formiranje biofilma kvasaca na abiotičkim površinama i rastuća otpornost na postojeće antimikrobne agense predstavilo je izazov za nas za pronalaženjem novih alternativnih agenasa biljnog porekla. Stoga mi smo ispitali antimikrobnu i antibiofilm aktivnost biljnih ekstrakata kao što su Humulus lupulus, Alpinia katsumadai i Evodia rutaecarpa prema C. albicans, C. glabrata i P. membranifaciens. Na osnovu dobijenih MIC vrednosti, testirani biljni ekstrakati su bili efikasni u inhibiciji kvasaca. Primećeno je da su biofilmovi C. glabrata pokazali veću otpornost na biljne ekstrakte u odnosu na C. albicans. Međutim, ekstrakti A. katsumadai i E. rutaecarpa su promovisani rast i razvoj formiranog biofilma P. membranifaciens., The adhesion and biofilm formation of yeast on the contact surfaces of the material is a serious hygiene problem in the food industry, because it can be a source of contamination during production, leading to major economic losses due to food spoilage and reduced product quality, and can also affects the consumer’s health. On the other hand, many infections in the hospital are caused by the adhesion of Candida spp. to medical devices and implants. However, the understanding of adhesion and colonization of yeasts on abiotic surfaces is limited to only some Candida species, and in particular little is known about Pichia species as adhesive agents.The aim of the research in this doctoral dissertation was to assess the potential of Candida and Pichia strains to adhere to polystyrene surface, stainless steel discs (AISI 304, Ra = 25.20 - 961.9 nm) and wood (beech) with different degrees of surface roughness, and examined the influence of surface roughness on yeast adhesion. Cell surface hydrophobicity (CSH) of Candida and Pichia strains was determined in order to assess the correlation between the cell surface hydrophobicity and the yeast adhesion to polystyrene. In addition, the influence of different medium, temperature, and various plant extracts on the adhesion of selected Candida and Pichia strains to to stainless steel surfaces was examined. Our results indicated that all Candida and Pichia strains were able to adhere to polystyrene, stainless steel surfaces and wood in a species- and strain-dependent manner. C. albicans had a higher ability to adhere to polystyrene and stainless steel compared to C. glabrata, C. parapsilosis and C. krusei. Regarding Pichia species, P. membranifaciens strains were less adherent to stainless steel than P. pijperi, what is not the case in the adhesion to polystyrene. Based on the results obtained, C. glabrata, C. parapsilosis, C. krusei, P. pijperi i P. membranifaciens strains were strongly to moderately hydrophobic while only C. albicans was moderately hydrophilic, indicating that the cell surface hydrophobicity is not significantly correlated with the amount of cells adhered to polystyrene. In addition, the adhesion of Candida and Pichia strains was significantly influenced by the roughness of stainless steel surface, while the roughness of wood surfaces did not significantly influence. Further on we examined the impact of different growth medium and temperature on adhesion of selected yeasts. It was noted that the amount of adhered cells of C. albicans and C. glabrata on stainless steel surfaces were significantly higher in the Malt Extract Broth (MEB) medium, whereas for C. parapsilosis, C. krusei, P. pijperi and P. membranifaciens it was Yeast Peptone Dextrose (YPD) medium. Our data also indicated that temperature is a very important factor which considerably affects the adhesion of these yeast. The problem of yeast adhesion and biofilm formation on abiotic surfaces and its growing resistance to existing antimicrobial agents, presented a challenge for us to find new alternative agents of plant origin. Hence, we investigated antimicrobial and antibiofilm activity of plant extracts such as Humulus lupulus, Alpinia katsumadai and Evodia rutaecarpa against C. albicans, C. glabrata and P. membranifaciens. According to the MIC values, all plant extracts were effective in the inhibition of yeast strains. It was observed that biofilms of C. glabrata are more resistance to plant extracts as compared to C. albicans. However, extracts of A. katsumadai and E. rutaecarpa were promoted the growth and development of a preformed biofilm of P. membranifaciens.
- Published
- 2018
17. Influence of growth conditions on adhesion of yeast Candida spp. and Pichia spp. to stainless steel surfaces
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Tomičić, Ružica, primary and Raspor, Peter, additional
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- 2017
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18. Sestrinstvo u Hrvatskoj
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Tomičić, Ružica
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Sestrinstvo ,Hrvatska - Abstract
Sestrinstvo u Hrvatskoj
- Published
- 2012
19. Adhesion of Candida spp. and Pichia spp. to Wooden Surfaces.
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Tomičić, Ružica, Tomičić, Zorica, and Raspor, Peter
- Subjects
ADHESION ,CANDIDA ,PICHIA ,FOOD spoilage ,METHYLENE blue - Abstract
Yeast adhesion to and biofilm formation on surfaces is present in many different environments. In food industry, bio films may be a source of contaminations, causing food spoilage and reducing quality of products. Candida and Pichia are two common yeast genera involved in the spoilage of some food products. The aim of this study is to assess the potential of Candida and Pichia species to adhere to two types of wooden surfaces (smooth and rough), one of the materials typical for the food processing industry, and investigate the influence of surface roughness of wood on the degree of yeast adhesion. The adhesion of the cells to the wooden surfaces was determined by rinsing them from the surface, followed by methylene blue staining, and quantification after imaging under microscope by automatic counting of viable cells. The results showed that all Candida and Pichia strains were able to adhere to the wooden surfaces in a species- and strain-dependent manner. On the other hand, our data indicated that adhesion by these yeasts was not significantly affected by the roughness of the wood surfaces. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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20. Germicidal efficacy of disinfectant based on sodium hypochlorite and essential oils.
- Author
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TOMIČIĆ, ZORICA, ČABARKAPA, IVANA, ŠARIĆ, LJUBIŠA, DŽOMBA, MARINA, and TOMIČIĆ, RUŽICA
- Subjects
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ESSENTIAL oils , *SODIUM hypochlorite , *SALMONELLA typhimurium , *PATHOGENIC bacteria , *STAPHYLOCOCCUS epidermidis , *BACILLUS cereus , *ANTI-infective agents - Abstract
The use of natural substances with antimicrobial activity in combination with conventional antimicrobial agents is a promising strategy in the control of pathogenic bacteria. The aim of this study was to examine the antimicrobial activity of a disinfectant based on sodium hypochlorite and lavender, tea tree, peppermint and rosemary essential oils against Gram-positive (Bacillus cereus ATCC 11778, Staphylococcus epidermidis ATCC 12228) and Gram-negative (Pseudomonas aeruginosa ATCC 10145, Salmonella Typhimurium ATCC 14028) bacteria. Further, to evaluate the antimicrobial activity of essential oils and the disinfectant mixed in various combinations in order to detect synergistic or antagonistic effects and discuss these effects on the basis of an improved checkerboard technique. The results showed a synergistic and additive effect for most combinations of the disinfectant and essential oils with fractional inhibitory concentrations index (FICI) values of 0.375 and 1, respectively. The effectiveness of the disinfectant in combination with essential oils was also determined using the quantitative suspension test and results indicated the maximum germicidal effect after the exposure time of 5 min. Taking into account increased interest in the use of natural antimicrobial agents in recent years, the application of a combination of disinfectant with essential oils represents a significant strategy in the control of pathogenic bacteria. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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