65 results on '"Tomasi, JP."'
Search Results
2. La physiopathogénie des cholestéatomes.
- Author
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Service de microbiologie, Gersdorff, Michel, Debaty, M E, Tomasi, JP., UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Service de microbiologie, Gersdorff, Michel, Debaty, M E, and Tomasi, JP.
- Abstract
OBJECTIVE: To describe the development of cholesteatoma using current knowledge. METHOD: Review of the literature. RESULTS: Cholesteatoma describes a mass of keratin (skin) in the middle ear which consists of a perimatrix and matrix. There are at least three kinds of cholesteatoma in the middle ear one resulting from invagination (retraction's pocket), another from migration and the last one from congenital inclusion. Cholesteatoma needs three successive inflammatory phases, the first leading to a retraction pocket, the second leading to pathology of the epidermis and of the floor of the external auditory canal and the third is the actual phase of cholesteatoma with invasion and middle ear auto-destruction with bone resorption. In this last phase, many factors play a role, collagenasis, osteoclats, cytokines, NO, bacteria and their biofilm and rupture of the retraction pocket. CONCLUSION: Cholesteatoma is an inflammatory disease of the ear caracterised by bone resorption. Current research is starting to appreciate the important role the immune system plays in the pathophysiology of cholesteatoma.
- Published
- 2006
3. No evidence of auditory dysfunction in guinea pigs immunized with myelin P0 protein.
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UCL - (SLuc) Service de neurologie, UCL - MD/MNOP - Département de morphologie normale et pathologique, UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Centre de référence pour l'épilepsie réfractaire, UCL - (SLuc) Service de microbiologie, Boulassel, M R, de Tourtchaninoff, Marianne, Guerit, Jean-Michel, Denison, S, Wenderickx, L, Botterman, Nicole, Deggouj, Naima, Gersdorff, Michel, Tomasi, JP., UCL - (SLuc) Service de neurologie, UCL - MD/MNOP - Département de morphologie normale et pathologique, UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Centre de référence pour l'épilepsie réfractaire, UCL - (SLuc) Service de microbiologie, Boulassel, M R, de Tourtchaninoff, Marianne, Guerit, Jean-Michel, Denison, S, Wenderickx, L, Botterman, Nicole, Deggouj, Naima, Gersdorff, Michel, and Tomasi, JP.
- Abstract
Recent data have focused on the peripheral nerve myelin glycoprotein P0 as a putative autoantigen involved in the autoimmune etiology of some cases of Meniere's disease, idiopathic sensorineural hearing loss and sudden deafness. To determine whether antibodies to myelin P0 can alter cochlear function, 13 healthy guinea pigs were immunized with purified porcine myelin P0 while 10 controls were injected with saline water. The animals were then evaluated for evidence of evolving inner ear disease using immunological, electrophysiological and morphological methods. Twenty-six experimental ears were tested weekly with a brainstem auditory evoked potential technique for a period of 4 months and were compared to 20 control ears. Uniformly, all P0-sensitized guinea pigs showed antibodies to myelin protein P0 as evidenced by ELISA. Clinical signs of inflammatory demyelination were not discernible in P0-sensitized guinea pigs and all the animals were qualitatively normal. No significant increase of evoked potential thresholds was found in the P0-sensitized animals when compared to controls (P>0.05). Peak latencies of waves I, II, III, IV and V and inter-peak latencies in P0-sensitized guinea pigs did not significantly differ from those of controls (P>0.05). Histological sections of inner ear and peripheral nerves were free of disease in both groups. These findings indicate that the sole presence of antibodies to myelin P0 in the sera of guinea pigs or patients suspected of having autoimmune inner ear diseases is unlikely to elicit auditory abnormalities and that additional factors are necessary for the pathogenic development of these disorders.
- Published
- 2001
4. Inner ear autoantibodies and their targets in patients with autoimmune inner ear diseases.
- Author
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Service de microbiologie, Boulassel, M R, Deggouj, Naima, Tomasi, JP., Gersdorff, Michel, UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Service de microbiologie, Boulassel, M R, Deggouj, Naima, Tomasi, JP., and Gersdorff, Michel
- Abstract
Immunological mechanisms are thought to play an important role in the pathogenesis of some cochleo-vestibular diseases. This study attempts to present further evidence of autoantibodies reactive against guinea pig inner ear proteins found in patients with autoimmune inner ear diseases (AIED) and specifically identifies the main target antigens of these antibodies. Sera from 110 patients with a clinical diagnosis of either rapidly progressive sensorineural hearing loss (n = 32). Ménière's disease (n = 41), sudden deafness (n = 6) or other aetiologies of hearing loss (n = 11) were screened by the Western blot technique. Forty-four percent of the patients' sera had antibodies to several inner ear proteins, of which the 30, 42 and 68 kDa proteins were found to be the most reactive. These highly reactive proteins were identified by gas-phase micro sequencing after digestion with trypsin and separation of peptide fragments by high-performance liquid chromatography. A partial sequence of each protein was determined. These data, together with those obtained from 2-dimensional gel electrophoresis followed by Western blotting, demonstrated that the 30 and 42 kDa inner ear proteins are the major peripheral myelin protein P0 and the beta-actin protein, respectively, while sequence analysis indicated that the 68 kDa protein is novel. These findings further support the hypothesis that several populations of antibodies may contribute to the enhanced immunological activity of AIED patients. They also add a new dimension to our knowledge of AIED and may open new avenues in the development of simple serological assays, which are easier to perform and more rapid than Western blotting.
- Published
- 2001
5. COCH5B2 is a target antigen of anti-inner ear antibodies in autoimmune inner ear diseases.
- Author
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Service de microbiologie, Boulassel, M R, Tomasi, JP., Deggouj, Naima, Gersdorff, Michel, UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Service de microbiologie, Boulassel, M R, Tomasi, JP., Deggouj, Naima, and Gersdorff, Michel
- Abstract
OBJECTIVE: This study was designed to identify the 58-kDa inner ear protein against which the sera of some patients with idiopathic, progressive sensorineural hearing loss or Ménière's disease strongly react. BACKGROUND: We and other groups have previously demonstrated that a 58-kDa protein extracted from guinea pig or bovine inner ear tissue is a target of antibodies in serum samples from some patients with autoimmune inner ear diseases. METHODS: After separation of inner ear proteins by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, the bands corresponding to 58 kDa were localized and excised from the gel. The concentrated protein was then digested with trypsin, and the peptide fragments were separated by high-pressure liquid chromatography. Three fractions were subjected to amino acid sequencing by the classic Edman degradation. RESULTS: The sequence of a stretch of 14 amino acids of the first fragment was identical to that of amino acids 526 to 539 of the COCH5B2 protein. The sequences of 11 and 10 amino acids of the second and third fragments, respectively, also were identical to residues 417 to 427 and 396 to 405 of the COCH5B2 protein. These data, together with two-dimensional gel electrophoresis followed by Western blot experiments, confirmed that the 58-kDa inner ear protein is the COCH5B2 protein. DISCUSSION: These findings indicate that the 58-kDa target protein of antibodies in serum samples of patients with autoimmune inner ear diseases is the COCH5B2 protein, a molecule that is highly and specifically expressed in the cochlea and vestibule.
- Published
- 2001
6. Autoimmune sensorineural hearing loss in young patients: an exploratory study.
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Service de microbiologie, UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique, Tomasi, JP., Lona, A, Deggouj, Naima, Gersdorff, Michel, UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, UCL - (SLuc) Service de microbiologie, UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique, Tomasi, JP., Lona, A, Deggouj, Naima, and Gersdorff, Michel
- Abstract
OBJECTIVES: P0 protein is expressed exclusively in myelinating Schwann cells of the peripheral nervous system. In a previous study from our laboratory, 27% of patients with sensorineural hearing loss (SNHL) had antibodies to P0 protein in their serum. The purpose of the present exploratory study was to examine the relationship between the clinical presentation of SNHL among children and young adults (age range, 5-30 y) and the presence of serum anti-P0 antibodies. STUDY DESIGN: The data were collected by retrospective questionnaires from Belgian otolaryngologists. METHODS: Patients were divided for comparison into two groups according to the presence or absence of anti-P0 antibodies. RESULTS: Analyses of clinical data and audiometric results indicated that a progressive hearing loss was more frequently recorded in the patients in the anti-P0 antibody-positive group (82% [14 of 17]) than in those in the anti-P0 antibody-negative group (35% [6 of 17]) (P <.005). CONCLUSIONS: Thus, in the age group in the present study, autoimmune SNHL (as measured in the present study by the presence of anti-P0 antibodies) is more frequently associated with progressive than with sudden hearing loss. The implications of this finding for preventive screening of hearing loss in children and young adults are discussed.
- Published
- 2001
7. Identification of beta-actin as a candidate autoantigen in autoimmune inner ear disease.
- Author
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, Boulassel, M R, Tomasi, JP., Deggouj, Naima, Gersdorff, Michel, UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, Boulassel, M R, Tomasi, JP., Deggouj, Naima, and Gersdorff, Michel
- Abstract
It has been shown that sera from patients with autoimmune inner ear disease contain antibodies to several inner ear antigens. We report here the characterization of the 42-43 kDa protein against which a significant number of patients' sera react strongly. After separation of inner ear proteins from guinea-pig cochleas by SDS-PAGE, the band corresponding to the 42-43 kDa protein was digested with trypsin and the peptide fragments were separated by high-performance liquid chromatography. Two fractions were then subjected to amino acid sequencing by the classical automated Edman degradation. The sequence of a stretch of 15 amino acids of the first fragment was identical to that of amino acids 148-162 of beta-actin. The sequence of the 10 amino acids of the second fragment was also identical to beta-actin. On Western blots, monoclonal antibody directed against beta-actin reacted with the inner ear 42-43 kDa proteins. The serum samples from the patients and the monoclonal antibody reacted with the non-muscle actin used as antigen in Western blotting. Immunoblot analysis of inner ear proteins after two-dimensional gel electrophoresis showed a spot, corresponding to the region of the 43 kDa as compared to the protein standards. On the basis of these data it is concluded that the target 42-43 kDa protein for antibodies in sera of patients with autoimmune inner ear disease is beta-actin, a molecule, which has important and numerous functions inside cells. This is the first report to identify the cytoskeletal protein beta-actin as a candidate autoantigen in autoimmune inner ear disease.
- Published
- 2000
8. Myelin protein Po as a potential autoantigen in autoimmune inner ear disease.
- Author
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - MD/BICL - Département de biochimie et de biologie cellulaire, UCL - (SLuc) Service d'oto-rhino-laryngologie, Cao, M Y, Dupriez, V J, Rider, Mark, Deggouj, Naima, Gersdorff, Michel, Rousseau, Guy, Tomasi, JP., UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - MD/BICL - Département de biochimie et de biologie cellulaire, UCL - (SLuc) Service d'oto-rhino-laryngologie, Cao, M Y, Dupriez, V J, Rider, Mark, Deggouj, Naima, Gersdorff, Michel, Rousseau, Guy, and Tomasi, JP.
- Abstract
We have previously shown that a 30,000 Mr protein extracted from guinea pig inner ear tissue is recognized by autoantibodies present in the serum of patients suffering from autoimmune inner ear disease. This protein was localized in the modiolus and in the organ of Corti. We have now identified this protein by a combination of microsequencing and matrix-assisted laser desorption ionization time-of-flight mass spectrometry of its tryptic peptides. A partial sequence of the protein was thereby determined. These data and 2-dimensional gel electrophoresis followed by immunoblotting experiments showed that the 30,000 Mr inner ear antigen is the major peripheral myelin protein Po. This suggests that protein Po may be an important autoantigen in autoimmune inner ear disease.
- Published
- 1996
9. Guinea pig inner ear antigens: extraction and application to the study of human autoimmune inner ear disease.
- Author
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, Cao, M Y, Deggouj, Naima, Gersdorff, Michel, Tomasi, JP., UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, Cao, M Y, Deggouj, Naima, Gersdorff, Michel, and Tomasi, JP.
- Abstract
In this study, the authors attempted to develop a method of extracting guinea pig inner ear antigens for otoimmunological research, and to investigate the distribution of the antigens in the various structures of the inner ear. The antigens were extracted either from the entire or from various parts of the guinea pig inner ear. These antigens were separated on sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Western blot techniques were used to test sera from patients with inner ear disease against guinea pig inner ear protein extracts. It found that the various molecular weight antigens in the inner ear were associated with the different structures of the inner ear. The sera of 37.5% (N = 80) of patients reacted with two bands (30 and 58 kd) of the guinea pig inner ear immunoblots. The 58 kd band was not specific to the inner ear, but instead was also found in the immunoblots of other guinea pig tissues (brain, lung, and liver). This study suggests that the various antigens of interest could be further extracted and purified from the corresponding locations of the inner ear.
- Published
- 1996
10. HLA class II-associated genetic susceptibility in idiopathic progressive sensorineural hearing loss.
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - MD/BICL - Département de biochimie et de biologie cellulaire, UCL - (SLuc) Service d'oto-rhino-laryngologie, Cao, M Y, Thonnard, J., Deggouj, Naima, Gersdorff, Michel, Philippe, Marianne, Osselaer, Jean-Claude, Tomasi, JP., UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - MD/BICL - Département de biochimie et de biologie cellulaire, UCL - (SLuc) Service d'oto-rhino-laryngologie, Cao, M Y, Thonnard, J., Deggouj, Naima, Gersdorff, Michel, Philippe, Marianne, Osselaer, Jean-Claude, and Tomasi, JP.
- Abstract
To investigate the association between genes in the major histocompatibility complex and inner ear disease susceptibility at the DNA level, high-resolution genotyping for HLA class II (HLA-DR, -DQ, -DP) was performed by polymerase chain reaction-sequence-specific oligonucleotide reverse dot blot and polymerase chain reaction-restriction fragment length polymorphism analysis in 34 patients with idiopathic progressive sensorineural hearing loss (PSHL) and in 214 controls. The frequencies of DRB1*0301, DRB3*0101, DQB1*0201, and DPB1*0401 were significantly increased in patients with idiopathic PSHL compared with controls. The DQB1*0301 allele was significantly decreased in the patients. A linkage disequilibrium was probably responsible for the concomitant increase of both DRB1*0301 and DRB3*0101 alleles in patients. The increase of DQB1*0201 in patients was associated with the DRB1*0301 allele. In addition, the telomeric DPB1*0401 allele may act as an independent risk factor. The DQB1*0301 allele may have a protective role in the pathogenesis of idiopathic PSHL. These results suggest that the specific HLA class II gene products may confer susceptibility or resistance to idiopathic PSHL.
- Published
- 1996
11. The localization and specificity of guinea pig inner ear antigenic epitopes.
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, Cao, M Y, Gersdorff, Michel, Deggouj, Naima, Tomasi, JP., UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service d'oto-rhino-laryngologie, Cao, M Y, Gersdorff, Michel, Deggouj, Naima, and Tomasi, JP.
- Abstract
In this study, we investigated the relative localization of some antigenic epitopes in the inner ear. The inner ear protein antigens were extracted from various parts of the guinea pig inner ear. Brain, kidney, lung, heart and liver extracts were also obtained. We found by SDS-polyacrylamide gel electrophoresis that total inner ear extracts separated into three high concentration polypeptide bands with molecular weights of approximately 30, 42, 58 kd and three low density bands of 20, 25 and 35 kd. The 30 kd band was found mainly in the extract of the spiral ganglion and the acoustic nerve in the modiolus. The 42 and 58 kd bands were detected in the extract of the spiral ligament and the stria vascularis. The Organ of Corti and the basilar membrane extract gave rise to three bands of 30, 42 and 58 kd. Twenty-eight of the 75 sera from patients with inner ear disease reacted with the 30 and 58 kd bands of the inner ear protein extracts by immunoblotting. Sixteen of these 28 positive sera were then used to probe immunoblots of the brain, kidney, lung, heart and liver extracts. The 58 kd band was also found in protein extracts of the brain, the lung and the liver. This study suggests that the 30 kd antigenic epitope may be mainly related to the acoustic nerve and that the 58 kd antigenic epitope is not cochlear specific.
- Published
- 1995
12. Anti-neutrophil antibodies in chronic hepatitis and the effect of alpha-interferon therapy.
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UCL - MD/MINT - Département de médecine interne, Warny, M., Brenard, R., Cornu, C., Tomasi, JP., Geubel, André, UCL - MD/MINT - Département de médecine interne, Warny, M., Brenard, R., Cornu, C., Tomasi, JP., and Geubel, André
- Abstract
In 34 patients with non-A non-B, 28 with type B and 11 with autoimmune chronic hepatitis, anti-neutrophil antibodies were investigated using indirect immunofluorescence and anti-myeloperoxidase antibodies by enzyme-linked immunosorbent assay. Granulocyte-specific antinuclear antibodies, were detected in 14 patients with advanced stages of non-A, non-B hepatitis (41%). Their presence correlated with histological features of disease activity but not with response to interferon therapy. Within 24 h after the first dose of interferon, 9 of these became negative and 3 more became negative after 1, 3 and 5 months. Myeloperoxidase-positive perinuclear neutrophil cytoplasmic antibodies were detected in a single patient and increased reaching a peak level after 8 weeks of interferon, decreasing thereafter. In type B, all were negative before and during the 6 months of therapy. In 6 patients with autoimmune hepatitis (55%), myeloperoxidase-negative perinuclear neutrophil cytoplasmic antibodies were detected in high titers. The association of granulocyte-specific anti-nuclear antibodies with non-A, non-B hepatitis support the hypothesis that hepatitis C virus infection might trigger humoral autoimmune response. In chronic autoimmune hepatitis, perinuclear neutrophil cytoplasmic antibodies appear as another marker of autoimmunity.
- Published
- 1993
13. Sjogrens-syndrome Associated With Crest and Positive Antibodies To Ro(ssa)-la(ssb)
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service de neurologie, Lambert, Michel, Tomasi, JP., Peeters, André, UCL - MD/NOPS - Département de neurologie et de psychiatrie, UCL - (SLuc) Service de neurologie, Lambert, Michel, Tomasi, JP., and Peeters, André
- Published
- 1993
14. [Severe Acute Hepatitis With Encephalopathy Induced By Acetylsalicylic-acid in Systemic Lupus-erythematosus]
- Author
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UCL - Cliniques universitaires Saint-Luc, UCL - MD/MINT - Département de médecine interne, UCL - MD/MNOP - Département de morphologie normale et pathologique, Deleeuw, P., Lefebvre, Chantal, Rahier, Jacques, Geubel, André, Tomasi, JP., UCL - Cliniques universitaires Saint-Luc, UCL - MD/MINT - Département de médecine interne, UCL - MD/MNOP - Département de morphologie normale et pathologique, Deleeuw, P., Lefebvre, Chantal, Rahier, Jacques, Geubel, André, and Tomasi, JP.
- Abstract
The observation of a 62 year-old woman who had been suffering from arthritis for several years and in whom treatment with high doses of acetylsalicyclic acid resulted in severe acute hepatitis is reported. Clinical and serological findings led to the diagnosis of systemic lupus erythematosus complicated by acute drug induced hepatitis.
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- 1992
15. [Comparative-study of Toxocariasis in Belgium and in Other Countries]
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UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique, Cauchie, V., Chaillet, P., Bigaignon, Geoffroy, Tomasi, JP., Vervoort, T., UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique, Cauchie, V., Chaillet, P., Bigaignon, Geoffroy, Tomasi, JP., and Vervoort, T.
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- 1990
16. A Clinical and Sero-epidemiological Study of 190 Belgian Patients Suffering From Lyme Borreliosis
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UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique, UCL - MD/NOPS - Département de neurologie et de psychiatrie, Bigaignon, Geoffroy, Tomasi, JP., Goubau, Patrick, Martin, Pascaline, Piérard, Denis, Sindic, Christian, Dupuis, Michel, Marcelis, L., Degreef, H., Willocx, D., Grillet, B., Westhovens, Rene, Roger, F., UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique, UCL - MD/NOPS - Département de neurologie et de psychiatrie, Bigaignon, Geoffroy, Tomasi, JP., Goubau, Patrick, Martin, Pascaline, Piérard, Denis, Sindic, Christian, Dupuis, Michel, Marcelis, L., Degreef, H., Willocx, D., Grillet, B., Westhovens, Rene, and Roger, F.
- Published
- 1989
17. Elisa Using Whole Legionella-pneumophila Cell As Antigen - Comparison Between Mono-valent and Polyvalent Antigens for the Serodiagnosis of Human Legionellosis
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UCL, Barka, N., Tomasi, JP., Stadtsbaeder, S., UCL, Barka, N., Tomasi, JP., and Stadtsbaeder, S.
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- 1986
18. [Legionella Infection in Hematology]
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UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique, Elias, E., Delmée, Michel, Tomasi, JP., Wauters, Georges, Michaux, JL., UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique, Elias, E., Delmée, Michel, Tomasi, JP., Wauters, Georges, and Michaux, JL.
- Published
- 1986
19. Serodiagnosis of Human G-immunoglobulins and M-immunoglobulins To Toxoplasma-gondii By Elisa Using Whole Tachyzoites As Antigens - a Comparative-study With the Indirect Hemagglutination (iha) and Immunofluorescence (ifa) Tests
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UCL, Tomasi, JP., Barka, N., Stadtsbaeder, S., UCL, Tomasi, JP., Barka, N., and Stadtsbaeder, S.
- Published
- 1986
20. Rapid Double-sandwich Enzyme-linked-immunosorbent-assay for Detection of Human Immunoglobulin-m Anti-toxoplasma-gondii Antibodies
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UCL, Tomasi, JP., Schlit, AF., Stadtsbaeder, S., UCL, Tomasi, JP., Schlit, AF., and Stadtsbaeder, S.
- Published
- 1986
21. Use of Whole Streptococcus-pneumoniae Cells As a Solid-phase Sorbent for C-reactive Protein Measurement By Elisa
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UCL, Barka, N., Tomasi, JP., Stadtsbaeder, S., UCL, Barka, N., Tomasi, JP., and Stadtsbaeder, S.
- Published
- 1985
22. Automated Particle-counting Immunoassay of C-reactive Protein and its Application To Serum, Cord Serum, and Cerebrospinal-fluid Samples
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UCL - MD/NOPS - Département de neurologie et de psychiatrie, Colletcassart, D., Mareschal, JC., Sindic, Christian, Tomasi, JP., Masson, PL., UCL - MD/NOPS - Département de neurologie et de psychiatrie, Colletcassart, D., Mareschal, JC., Sindic, Christian, Tomasi, JP., and Masson, PL.
- Published
- 1983
23. Revised 2017 international consensus on ANCA testing in small vessel vasculitis: support from an external quality assessment.
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Broeders S, Goletti S, Tomasi JP, Bonroy C, Humbel RL, Lutteri L, Schouwers S, Van Hoovels L, Vercammen M, and Bossuyt X
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- Consensus, Fluorescent Antibody Technique, Indirect, Humans, Immunoassay, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis, Antibodies, Antineutrophil Cytoplasmic
- Abstract
Competing Interests: Competing interests: XB has been a consultant to Inova Diagnostics and to Thermo Fisher.
- Published
- 2019
- Full Text
- View/download PDF
24. Belgian recommendations on ANA, anti-dsDNA and anti-ENA antibody testing.
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Van Blerk M, Bossuyt X, Humbel R, Mewis A, Servais G, Tomasi JP, Van Campenhout C, Van Hoovels L, Vercammen M, Damoiseaux J, Coucke W, and Van de Walle P
- Subjects
- Belgium, Cell Line, DNA immunology, Humans, Laboratories statistics & numerical data, Practice Guidelines as Topic, Reference Values, Surveys and Questionnaires, Antibodies, Antinuclear blood, Fluorescent Antibody Technique, Indirect standards, Laboratories standards
- Abstract
Autoantibodies to nuclear antigens, i.e. antinuclear antibodies (ANA), antibodies to double-stranded DNA (dsDNA) and extractable nuclear antigens (ENA), are useful as diagnostic markers for a variety of autoimmune diseases. In March 2010, the Belgian national External Quality Assessment Scheme sent a questionnaire on ANA, anti-dsDNA and anti-ENA antibody testing designed by the Dutch EASI (European Autoimmunity Standardization Initiative) team, to all clinical laboratories performing ANA testing. Virtually all laboratories completed the questionnaire (97·7%, 127/130). This paper discusses the results of this questionnaire and provides valuable information on the state-of-the-art of ANA, anti-dsDNA and anti-ENA antibody testing as practiced in the Belgian laboratories. In addition, this work presents practical recommendations developed by the members of the advisory board of the scheme as a result of the outcome of this study.
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- 2014
- Full Text
- View/download PDF
25. Lack of evidence of stimulatory autoantibodies to platelet-derived growth factor receptor in patients with systemic sclerosis.
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Classen JF, Henrohn D, Rorsman F, Lennartsson J, Lauwerys BR, Wikström G, Rorsman C, Lenglez S, Franck-Larsson K, Tomasi JP, Kämpe O, Vanthuyne M, Houssiau FA, and Demoulin JB
- Subjects
- Adult, Aged, Animals, Becaplermin, Biological Assay, Cell Line, Female, Humans, Immunoglobulin G blood, Male, Mice, Middle Aged, Platelet-Derived Growth Factor metabolism, Platelet-Derived Growth Factor pharmacology, Proto-Oncogene Proteins c-sis, Receptor, Platelet-Derived Growth Factor alpha genetics, Receptor, Platelet-Derived Growth Factor alpha metabolism, Receptor, Platelet-Derived Growth Factor beta genetics, Receptor, Platelet-Derived Growth Factor beta metabolism, Transfection, Autoantibodies blood, Receptor, Platelet-Derived Growth Factor alpha immunology, Receptor, Platelet-Derived Growth Factor beta immunology, Scleroderma, Systemic immunology
- Abstract
Objective: Systemic sclerosis (SSc) is a severe connective tissue disease of unknown etiology, characterized by fibrosis of the skin and multiple internal organs. Recent findings suggested that the disease is driven by stimulatory autoantibodies to platelet-derived growth factor receptor (PDGFR), which stimulate the production of reactive oxygen species (ROS) and collagen by fibroblasts. These results opened novel avenues of research into the diagnosis and treatment of SSc. The present study was undertaken to confirm the presence of anti-PDGFR antibodies in patients with SSc., Methods: Immunoglobulins from 37 patients with SSc were purified by protein A/G chromatography. PDGFR activation was tested using 4 different sensitive bioassays, i.e., cell proliferation, ROS production, signal transduction, and receptor phosphorylation; the latter was also tested in a separate population of 7 patients with SSc from a different research center., Results: Purified IgG samples from patients with SSc were positive when tested for antinuclear autoantibodies, but did not specifically activate PDGFRalpha or PDGFRbeta in any of the tests. Cell stimulation with PDGF itself consistently produced a strong signal., Conclusion: The present results raise questions regarding the existence of agonistic autoantibodies to PDGFR in SSc.
- Published
- 2009
- Full Text
- View/download PDF
26. Current practices in antinuclear antibody testing: results from the Belgian External Quality Assessment Scheme.
- Author
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Van Blerk M, Van Campenhout C, Bossuyt X, Duchateau J, Humbel R, Servais G, Tomasi JP, Albert A, Coucke W, and Libeer JC
- Subjects
- Belgium, Humans, Quality Assurance, Health Care, Reference Standards, Surveys and Questionnaires, Antibodies, Antinuclear analysis, Fluorescent Antibody Technique, Indirect standards
- Abstract
Background: This study aimed to assess the state-of-the-art of antinuclear antibody (ANA) testing as practiced in the Belgian and Luxembourg laboratories, using the results obtained in the Belgian National External Quality Assessment Scheme from 2000 to 2005., Methods: During this period, nine samples with different specificities were sent for analysis. Participants were surveyed for methodology used and were asked to report staining pattern and titer of ANAs. In 2002, an attempt was made to improve the comparability of quantitative ANA results by the provision of a commercial reference material and to relate observed differences to methodology., Results: With one exception, all participants employed a microscope-based indirect immunofluorescence assay with human epithelial cell line 2 cells. Most laboratories were accurate in describing the pattern. The percentage of unacceptable answers was greater for samples with borderline levels of antibody and for samples showing a cytoplasmic pattern. An improvement in the detection of anticentromere antibodies was observed. For all samples, a wide range of titers was reported. The provision of the secondary reference preparation led to improved inter-laboratory concordance. Comparison of methodology variables revealed a correlation between unstandardized titers and the power of the lamp of the microscope and the use of a dark room., Conclusions: The EQAS results presented in this work provide valuable insights into the state of the art of ANA testing as practiced in the Belgian and Luxembourg Laboratories and illustrate the important value of a national EQAS for ANA testing as a tool to improve performance and interlaboratory comparability of laboratory results.
- Published
- 2009
- Full Text
- View/download PDF
27. [Pathophysiology of cholesteatoma].
- Author
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Gersdorff MC, Debaty ME, and Tomasi JP
- Subjects
- Bacteria growth & development, Bacterial Infections immunology, Biofilms growth & development, Bone Resorption, Cholesteatoma, Middle Ear immunology, Cholesteatoma, Middle Ear microbiology, Cytokines immunology, Humans, Nitric Oxide physiology, Osteoclasts metabolism, Cholesteatoma, Middle Ear physiopathology
- Abstract
Objective: To describe the development of cholesteatoma using current knowledge., Method: Review of the literature., Results: Cholesteatoma describes a mass of keratin (skin) in the middle ear which consists of a perimatrix and matrix. There are at least three kinds of cholesteatoma in the middle ear one resulting from invagination (retraction's pocket), another from migration and the last one from congenital inclusion. Cholesteatoma needs three successive inflammatory phases, the first leading to a retraction pocket, the second leading to pathology of the epidermis and of the floor of the external auditory canal and the third is the actual phase of cholesteatoma with invasion and middle ear auto-destruction with bone resorption. In this last phase, many factors play a role, collagenasis, osteoclats, cytokines, NO, bacteria and their biofilm and rupture of the retraction pocket., Conclusion: Cholesteatoma is an inflammatory disease of the ear caracterised by bone resorption. Current research is starting to appreciate the important role the immune system plays in the pathophysiology of cholesteatoma.
- Published
- 2006
28. Prospective screening for biopsy proven coeliac disease, autoimmunity and malabsorption markers in Belgian subjects with Type 1 diabetes.
- Author
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Buysschaert M, Tomasi JP, and Hermans MP
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Autoantibodies analysis, Autoimmune Diseases complications, Autoimmune Diseases epidemiology, Autoimmune Diseases immunology, Belgium epidemiology, Biomarkers analysis, Biopsy, Celiac Disease complications, Celiac Disease epidemiology, Celiac Disease immunology, Diabetes Mellitus, Type 1 epidemiology, Diabetes Mellitus, Type 1 immunology, Female, Humans, Malabsorption Syndromes epidemiology, Malabsorption Syndromes immunology, Male, Mass Screening methods, Middle Aged, Prevalence, Prospective Studies, Autoimmunity immunology, Diabetes Mellitus, Type 1 complications, Malabsorption Syndromes complications
- Abstract
Aims: To determine prospectively the prevalence of biopsy proven coeliac disease (CD) in an adult Type 1 diabetic population from Belgium with regards to associated auto-immunity and malabsorption., Methods and Results: Determination in 400 Type 1 diabetic patients of serum anti-endomysial and/or anti-transglutaminase auto-antibodies. All subjects with abnormal serology underwent an intestinal biopsy. Ten patients (2.5%) had positive antibodies. Diagnosis of CD was confirmed by an intestinal biopsy. Eight patients were symptom-free, although laboratory findings suggesting malabsorption were prominent in the presence of CD [microcytic anaemia, iron and folate deficiencies, low levels of 25(OH)vitamin D3, calcium and cholesterol]. Other auto-immune conditions, especially vitiligo, were found in patients with CD., Conclusions: Asymptomatic coeliac disease occurs frequently in adult Type 1 diabetic patients, and is often associated with subclinical malabsorption. Screening should be part of routine evaluation, to implement life-long dietary gluten avoidance.
- Published
- 2005
- Full Text
- View/download PDF
29. Autoimmune inner ear disease: diagnostic and advanced treatments.
- Author
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Tomasi JP and Gersdorff M
- Subjects
- Autoimmune Diseases immunology, Ear Diseases immunology, Humans, Autoimmune Diseases diagnosis, Autoimmune Diseases therapy, Ear Diseases diagnosis, Ear Diseases therapy, Ear, Inner immunology
- Published
- 2002
30. Autoimmune sensorineural hearing loss in young patients: an exploratory study.
- Author
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Tomasi JP, Lona A, Deggouj N, and Gersdorff M
- Subjects
- Adolescent, Adult, Case-Control Studies, Child, Child, Preschool, Female, Humans, Immunoblotting, Male, Autoantibodies blood, Autoimmune Diseases immunology, Hearing Loss, Sensorineural immunology, Myelin P0 Protein immunology
- Abstract
Objectives: P0 protein is expressed exclusively in myelinating Schwann cells of the peripheral nervous system. In a previous study from our laboratory, 27% of patients with sensorineural hearing loss (SNHL) had antibodies to P0 protein in their serum. The purpose of the present exploratory study was to examine the relationship between the clinical presentation of SNHL among children and young adults (age range, 5-30 y) and the presence of serum anti-P0 antibodies., Study Design: The data were collected by retrospective questionnaires from Belgian otolaryngologists., Methods: Patients were divided for comparison into two groups according to the presence or absence of anti-P0 antibodies., Results: Analyses of clinical data and audiometric results indicated that a progressive hearing loss was more frequently recorded in the patients in the anti-P0 antibody-positive group (82% [14 of 17]) than in those in the anti-P0 antibody-negative group (35% [6 of 17]) (P <.005)., Conclusions: Thus, in the age group in the present study, autoimmune SNHL (as measured in the present study by the presence of anti-P0 antibodies) is more frequently associated with progressive than with sudden hearing loss. The implications of this finding for preventive screening of hearing loss in children and young adults are discussed.
- Published
- 2001
- Full Text
- View/download PDF
31. COCH5B2 is a target antigen of anti-inner ear antibodies in autoimmune inner ear diseases.
- Author
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Boulassel MR, Tomasi JP, Deggouj N, and Gersdorff M
- Subjects
- Animals, Blotting, Western, Chromatography, High Pressure Liquid methods, Electrophoresis, Agar Gel methods, Female, Guinea Pigs, Male, Antibodies immunology, Ear, Inner immunology, Ear, Inner metabolism, Hearing Loss, Sensorineural immunology, Meniere Disease immunology, Meniere Disease metabolism, Proteins immunology, Proteins metabolism
- Abstract
Objective: This study was designed to identify the 58-kDa inner ear protein against which the sera of some patients with idiopathic, progressive sensorineural hearing loss or Ménière's disease strongly react., Background: We and other groups have previously demonstrated that a 58-kDa protein extracted from guinea pig or bovine inner ear tissue is a target of antibodies in serum samples from some patients with autoimmune inner ear diseases., Methods: After separation of inner ear proteins by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, the bands corresponding to 58 kDa were localized and excised from the gel. The concentrated protein was then digested with trypsin, and the peptide fragments were separated by high-pressure liquid chromatography. Three fractions were subjected to amino acid sequencing by the classic Edman degradation., Results: The sequence of a stretch of 14 amino acids of the first fragment was identical to that of amino acids 526 to 539 of the COCH5B2 protein. The sequences of 11 and 10 amino acids of the second and third fragments, respectively, also were identical to residues 417 to 427 and 396 to 405 of the COCH5B2 protein. These data, together with two-dimensional gel electrophoresis followed by Western blot experiments, confirmed that the 58-kDa inner ear protein is the COCH5B2 protein., Discussion: These findings indicate that the 58-kDa target protein of antibodies in serum samples of patients with autoimmune inner ear diseases is the COCH5B2 protein, a molecule that is highly and specifically expressed in the cochlea and vestibule.
- Published
- 2001
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- View/download PDF
32. No evidence of auditory dysfunction in guinea pigs immunized with myelin P0 protein.
- Author
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Boulassel MR, Guérit JM, Denison S, de Tourtchaninoff M, Wenderickx L, Botterman N, Deggouj N, Gersdorff M, and Tomasi JP
- Subjects
- Animals, Antibodies analysis, Auditory Threshold physiology, Guinea Pigs, Reaction Time physiology, Spiral Ganglion pathology, Swine, Temporal Bone pathology, Hearing Disorders immunology, Immunization, Myelin P0 Protein immunology
- Abstract
Recent data have focused on the peripheral nerve myelin glycoprotein P0 as a putative autoantigen involved in the autoimmune etiology of some cases of Meniere's disease, idiopathic sensorineural hearing loss and sudden deafness. To determine whether antibodies to myelin P0 can alter cochlear function, 13 healthy guinea pigs were immunized with purified porcine myelin P0 while 10 controls were injected with saline water. The animals were then evaluated for evidence of evolving inner ear disease using immunological, electrophysiological and morphological methods. Twenty-six experimental ears were tested weekly with a brainstem auditory evoked potential technique for a period of 4 months and were compared to 20 control ears. Uniformly, all P0-sensitized guinea pigs showed antibodies to myelin protein P0 as evidenced by ELISA. Clinical signs of inflammatory demyelination were not discernible in P0-sensitized guinea pigs and all the animals were qualitatively normal. No significant increase of evoked potential thresholds was found in the P0-sensitized animals when compared to controls (P>0.05). Peak latencies of waves I, II, III, IV and V and inter-peak latencies in P0-sensitized guinea pigs did not significantly differ from those of controls (P>0.05). Histological sections of inner ear and peripheral nerves were free of disease in both groups. These findings indicate that the sole presence of antibodies to myelin P0 in the sera of guinea pigs or patients suspected of having autoimmune inner ear diseases is unlikely to elicit auditory abnormalities and that additional factors are necessary for the pathogenic development of these disorders.
- Published
- 2001
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- View/download PDF
33. Inner ear autoantibodies and their targets in patients with autoimmune inner ear diseases.
- Author
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Boulassel MR, Deggouj N, Tomasi JP, and Gersdorff M
- Subjects
- Animals, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Guinea Pigs, Hearing Loss, Sensorineural immunology, Hearing Loss, Sudden immunology, Humans, Meniere Disease immunology, Proteins immunology, Autoantibodies analysis, Autoimmune Diseases immunology, Ear, Inner immunology, Labyrinth Diseases immunology
- Abstract
Immunological mechanisms are thought to play an important role in the pathogenesis of some cochleo-vestibular diseases. This study attempts to present further evidence of autoantibodies reactive against guinea pig inner ear proteins found in patients with autoimmune inner ear diseases (AIED) and specifically identifies the main target antigens of these antibodies. Sera from 110 patients with a clinical diagnosis of either rapidly progressive sensorineural hearing loss (n = 32). Ménière's disease (n = 41), sudden deafness (n = 6) or other aetiologies of hearing loss (n = 11) were screened by the Western blot technique. Forty-four percent of the patients' sera had antibodies to several inner ear proteins, of which the 30, 42 and 68 kDa proteins were found to be the most reactive. These highly reactive proteins were identified by gas-phase micro sequencing after digestion with trypsin and separation of peptide fragments by high-performance liquid chromatography. A partial sequence of each protein was determined. These data, together with those obtained from 2-dimensional gel electrophoresis followed by Western blotting, demonstrated that the 30 and 42 kDa inner ear proteins are the major peripheral myelin protein P0 and the beta-actin protein, respectively, while sequence analysis indicated that the 68 kDa protein is novel. These findings further support the hypothesis that several populations of antibodies may contribute to the enhanced immunological activity of AIED patients. They also add a new dimension to our knowledge of AIED and may open new avenues in the development of simple serological assays, which are easier to perform and more rapid than Western blotting.
- Published
- 2001
- Full Text
- View/download PDF
34. No increased serum levels of antifood antibodies in patients with Ménière's disease.
- Author
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Boulassel MR, Alost M, Tomasi JP, Deggouj N, and Gersdorff M
- Subjects
- Adult, Aged, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Middle Aged, Surveys and Questionnaires, Allergens immunology, Food, Food Hypersensitivity immunology, Immunoglobulin A blood, Immunoglobulin G blood, Meniere Disease immunology
- Abstract
The etiology of Ménière's disease (MD) is still unknown, but it is likely to be multifactorial, one of the factors being an immunological causation. Antifood allergens as well as anti-baker's yeast antibodies are humoral factors that may be linked with allergenic disorders and other autoimmune conditions. To determine their possible role in MD activity, we investigated 29 MD sera for the presence of antibodies against gliadin, beta-lactoglobulin, albumin, ovalbumin, soya, and Dermatophagoides pteronyssinus and Saccharomyces cerevisiae strains using an ELISA technique. The patients were compared with 29 healthy individuals matched for sex and age. A serum was regarded as positive if the absorbance was two standard deviations higher than values obtained with sera from healthy subjects. Historical data, including factors which the patients believed to provoke their Ménière's symptoms, were obtained from patients' questionnaires. MD patients showed no significant symptoms of allergenic disorders suggesting allergies when compared to controls (p > 0.05). IgG and IgA antibody levels were not significantly raised in MD patients as compared with healthy controls (p > 0.05) for gliadin, beta-lactoglobulin, soya, albumin, ovalbumin, and D. pteronyssinus and S. cerevisiae strains. These data do not convincingly support a hypothesis of increased serum levels of antifood antibodies in patients with MD, as very few patients were antibody positive., (Copyright 2001 S. Karger AG, Basel)
- Published
- 2001
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- View/download PDF
35. Identification of beta-actin as a candidate autoantigen in autoimmune inner ear disease.
- Author
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Boulassel MR, Tomasi JP, Deggouj N, and Gersdorff M
- Subjects
- Animals, Blotting, Western, Carrier Proteins immunology, Female, Fluorescent Antibody Technique, Indirect, Guinea Pigs, Male, Actins immunology, Autoantibodies immunology, Autoantigens immunology, Ear Diseases immunology, Ear, Inner microbiology
- Abstract
It has been shown that sera from patients with autoimmune inner ear disease contain antibodies to several inner ear antigens. We report here the characterization of the 42-43 kDa protein against which a significant number of patients' sera react strongly. After separation of inner ear proteins from guinea-pig cochleas by SDS-PAGE, the band corresponding to the 42-43 kDa protein was digested with trypsin and the peptide fragments were separated by high-performance liquid chromatography. Two fractions were then subjected to amino acid sequencing by the classical automated Edman degradation. The sequence of a stretch of 15 amino acids of the first fragment was identical to that of amino acids 148-162 of beta-actin. The sequence of the 10 amino acids of the second fragment was also identical to beta-actin. On Western blots, monoclonal antibody directed against beta-actin reacted with the inner ear 42-43 kDa proteins. The serum samples from the patients and the monoclonal antibody reacted with the non-muscle actin used as antigen in Western blotting. Immunoblot analysis of inner ear proteins after two-dimensional gel electrophoresis showed a spot, corresponding to the region of the 43 kDa as compared to the protein standards. On the basis of these data it is concluded that the target 42-43 kDa protein for antibodies in sera of patients with autoimmune inner ear disease is beta-actin, a molecule, which has important and numerous functions inside cells. This is the first report to identify the cytoskeletal protein beta-actin as a candidate autoantigen in autoimmune inner ear disease.
- Published
- 2000
- Full Text
- View/download PDF
36. [Rectal leiomyosarcoma. 4 new cases].
- Author
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Cailliez-Tomasi JP, Bouché O, Avisse C, Ramaholimihaso F, Diébold MD, and Flament JB
- Subjects
- Aged, Aged, 80 and over, Antineoplastic Agents therapeutic use, Endosonography, Female, Humans, Leiomyosarcoma surgery, Leiomyosarcoma therapy, Male, Radiotherapy, Rectal Neoplasms surgery, Rectal Neoplasms therapy, Leiomyosarcoma diagnostic imaging, Rectal Neoplasms diagnostic imaging
- Abstract
We report four cases of leiomyosarcoma of the rectum suspected by endoscopic ultrasonography. Three patients were treated by local excision and one by abdominoperineal resection. An excision of the mass via a Kraske's approach was used. Leiomyosarcoma confined to the rectum wall can be treated by local excision. Endosonography can provide exact estimation of the lesion and is of great value in selecting the appropriate treatment. The treatment is surgical excision with wide margins. The histological stage and the presence or absence of metastases determine the therapeutic. Two patients in our series underwent radiation therapy. Chemotherapeutic agents including doxorubicin have had beneficial effect on recurrence or survival, only for higher grade sarcomas.
- Published
- 1999
37. Clinical significance of antiproteinase 3 antibody positivity in cANCA-positive patients.
- Author
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van Pesch V, Jadoul M, Lefèbvre C, Lauwerys BR, Tomasi JP, Devogelaer JP, and Houssiau FA
- Subjects
- Biomarkers blood, Diagnosis, Differential, Enzyme-Linked Immunosorbent Assay, Granulomatosis with Polyangiitis blood, Granulomatosis with Polyangiitis diagnosis, Humans, Myeloblastin, Retrospective Studies, Severity of Illness Index, Antibodies, Antineutrophil Cytoplasmic immunology, Autoantibodies analysis, Autoantigens immunology, Granulomatosis with Polyangiitis immunology, Serine Endopeptidases immunology
- Abstract
We addressed the clinical significance of antiproteinase 3 (anti-PR3) antibody (Ab) positivity by reviewing the files of 79 patients whose serum contained antineutrophil cytoplasmic antibodies with a cytoplasmic staining pattern (cANCA) and had been tested for anti-PR3 reactivity. Vasculitis was present in most (22/35) cANCA+ PR3+ patients but in only a few (5/44) cANCA+ PR3- patients, thereby suggesting that anti-PR3 Ab positivity in cANCA+ patients is more indicative of vasculitis than cANCA positivity alone. Noteworthy, one-third of cANCA+ PR3+ patients -- those with anti-PR3 Ab titres lower than 100 U/ml -- did not suffer from vasculitis. Anti-PR3 reactivity in vasculitis patients was only weakly associated with Wegener's granulomatosis (WG), as nine out of 22 cANCA+ PR3+ vasculitis patients (41%) did not fulfil the ACR classification criteria for WG. There was no correlation between anti-PR3 Ab titres and disease activity at diagnosis. However, titres measured when patients were in remission were much lower than initial values. Taken together, our results indicate that anti-PR3 Ab positivity should be interpreted in its clinical context.
- Published
- 1999
- Full Text
- View/download PDF
38. Right nonrecurrent inferior laryngeal nerve and arteria lusoria: the diagnostic and therapeutic implications of an anatomic anomaly. Review of 17 cases.
- Author
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Avisse C, Marcus C, Delattre JF, Marcus C, Cailliez-Tomasi JP, Palot JP, Ladam-Marcus V, Menanteau B, and Flament JB
- Subjects
- Adult, Aged, Female, Humans, Laryngeal Nerve Injuries, Magnetic Resonance Angiography, Male, Middle Aged, Postoperative Complications etiology, Subclavian Artery injuries, Thyroid Diseases etiology, Thyroid Diseases surgery, Thyroid Gland blood supply, Thyroid Gland innervation, Laryngeal Nerves abnormalities, Subclavian Artery abnormalities, Thyroid Diseases diagnosis, Thyroid Gland surgery
- Abstract
The authors report 17 cases of a right non-recurrent inferior laryngeal n. (NRILN) observed during 15 years of practice of thyroid and parathyroid surgery. In their last two cases, the existence of an aberrant right subclavian a., constantly associated with NRILN, was confirmed by MRI angiography. On the basis of the literature and their own experience, the authors review the incidence of this double anomaly, its embryologic explanation and its anatomic and surgical importance. They stress the diagnostic factors and the therapeutic implications, very different in children and adults, of a particular vascular anomaly whose outcome is little understood.
- Published
- 1998
39. [Digestive cancers and choroid metastasis. Diagnostic circumstances and prognostic value apropos of 2 cases].
- Author
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Cailliez-Tomasi JP, Avisse C, Bouché O, Palot JP, Ducasse A, and Flament JB
- Subjects
- Adenocarcinoma diagnostic imaging, Adenocarcinoma pathology, Aged, CA-125 Antigen analysis, CA-19-9 Antigen analysis, Choroid Neoplasms diagnostic imaging, Choroid Neoplasms pathology, Fatal Outcome, Female, Humans, Lymphatic Metastasis, Male, Neoplasm Invasiveness, Prognosis, Tomography, X-Ray Computed, Ultrasonography, Adenocarcinoma secondary, Choroid Neoplasms secondary, Pancreatic Neoplasms pathology, Stomach Neoplasms pathology
- Abstract
We report two observations of malignant gastric and pancreatic tumors with choroid metastasis. Clinical course was rapidly and spontaneously unfavorable.
- Published
- 1997
40. [Combination of colorectal tumor and Streptococcus bovis endocarditis. Apropos of 3 cases].
- Author
-
Cailliez-Tomasi JP, Avisse C, Baehrel B, Palot JP, and Flament JB
- Subjects
- Adenocarcinoma pathology, Adenocarcinoma therapy, Aged, Bacteremia drug therapy, Bacteremia microbiology, Colorectal Neoplasms pathology, Colorectal Neoplasms therapy, Endocarditis, Bacterial microbiology, Endocarditis, Bacterial therapy, Humans, Male, Middle Aged, Streptococcal Infections microbiology, Streptococcal Infections therapy, Treatment Outcome, Adenocarcinoma complications, Colorectal Neoplasms complications, Endocarditis, Bacterial complications, Streptococcal Infections complications, Streptococcus bovis
- Abstract
The authors report about 3 cases of bovis endocarditis revealing a colic carcinoma. This morbidity confirms the need for routine digestive investigations in endocarditis due to group D streptococci and eventually a liver disease.
- Published
- 1997
41. [Comments on emergency treatment of biliary lithiasis in patients over 75 years of age. Apropos of 157 cases].
- Author
-
Avisse C, Mancini F, Cailliez-Tomasi JP, Bouche O, Thiefin G, Burde A, Conce JP, Delattre JF, Palot JP, and Flament JB
- Subjects
- Aged, Aged, 80 and over, Cholecystostomy methods, Cholelithiasis diagnosis, Cholelithiasis mortality, Emergency Treatment adverse effects, Female, Follow-Up Studies, Humans, Male, Prognosis, Sphincterotomy, Endoscopic, Cholelithiasis therapy, Emergency Treatment methods
- Abstract
Surgery remains the ideal emergency treatment for biliary lithiasis in elderly subjects despite perioperative morbidity and mortality. Minimally invasive techniques appear promising but require assessment. The aim of this work was to determine the usefulness of these techniques and evaluate outcome in a series of 157 patients over 75 years of age who were hospitalized in an emergency setting of complicated biliary lithiasis from January 1990 to December 1996. There were 103 women and 54 men, mean age 82 years. The patients' general status was evaluated according to the ASA classification; 66% of the patients were ASA III, IV or V. Diagnoses at admission were acute cholecystitis (n = 71, 45%), angiocholitis (n = 50, 31%) subintrant hepatic colic (n = 17, 10.8%), pancreatitis (n = 10, 6%), isolated jaundice (n = 2), peritonitis (n = 2) and occlusion (n = 5). Within 24 hours of admission, 7 patients underwent emergency surgery, and the 150 others were given medical treatment. Among these 150 patients, cure was considered to have been achieved with medical treatment alone in 41 (subsequent surgery being required in only one 6 months later), semi-emergency was performed in 17, and a minimally invasive procedure was performed in the 92 others (echo-guided percutaneous cholecystostomy in 42, endoscopic sphincterotomy in 50) followed by a subsequent operation in 29. In the 103 patients (65.5%) in this series who did not undergo surgery, mortality was 3.8% and in the 54 patients (34.5%) who did, mortality was 15%, but this rate was only 6.9% when the open procedure followed a minimally invasive technique. Surgical treatment of complicated biliary disease remains the ideal therapy but indications should be carefully weighed in these elderly fragilized subjects. Under surgical observation, abstention from surgery or use of minimally invasive techniques can play an important role in the therapeutic strategy aimed at lowering perioperative mortality.
- Published
- 1997
42. Myelin protein Po as a potential autoantigen in autoimmune inner ear disease.
- Author
-
Cao MY, Dupriez VJ, Rider MH, Deggouj N, Gersdorff MC, Rousseau GG, and Tomasi JP
- Subjects
- Amino Acid Sequence, Animals, Ear, Middle immunology, Female, Guinea Pigs, Humans, Male, Molecular Sequence Data, Peptide Mapping, Autoantigens immunology, Autoimmune Diseases immunology, Ear Diseases immunology, Myelin P0 Protein immunology
- Abstract
We have previously shown that a 30,000 Mr protein extracted from guinea pig inner ear tissue is recognized by autoantibodies present in the serum of patients suffering from autoimmune inner ear disease. This protein was localized in the modiolus and in the organ of Corti. We have now identified this protein by a combination of microsequencing and matrix-assisted laser desorption ionization time-of-flight mass spectrometry of its tryptic peptides. A partial sequence of the protein was thereby determined. These data and 2-dimensional gel electrophoresis followed by immunoblotting experiments showed that the 30,000 Mr inner ear antigen is the major peripheral myelin protein Po. This suggests that protein Po may be an important autoantigen in autoimmune inner ear disease.
- Published
- 1996
- Full Text
- View/download PDF
43. HLA class II-associated genetic susceptibility in idiopathic progressive sensorineural hearing loss.
- Author
-
Cao MY, Thonnard J, Deggouj N, Gersdorff M, Philippe M, Osselaer JC, and Tomasi JP
- Subjects
- Adult, Autoimmune Diseases genetics, Base Sequence, Female, Hearing Loss, Sensorineural diagnosis, Humans, Male, Middle Aged, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Genetic, HLA-DQ Antigens genetics, HLA-DR Antigens genetics, Hearing Loss, Sensorineural genetics
- Abstract
To investigate the association between genes in the major histocompatibility complex and inner ear disease susceptibility at the DNA level, high-resolution genotyping for HLA class II (HLA-DR, -DQ, -DP) was performed by polymerase chain reaction-sequence-specific oligonucleotide reverse dot blot and polymerase chain reaction-restriction fragment length polymorphism analysis in 34 patients with idiopathic progressive sensorineural hearing loss (PSHL) and in 214 controls. The frequencies of DRB1*0301, DRB3*0101, DQB1*0201, and DPB1*0401 were significantly increased in patients with idiopathic PSHL compared with controls. The DQB1*0301 allele was significantly decreased in the patients. A linkage disequilibrium was probably responsible for the concomitant increase of both DRB1*0301 and DRB3*0101 alleles in patients. The increase of DQB1*0201 in patients was associated with the DRB1*0301 allele. In addition, the telomeric DPB1*0401 allele may act as an independent risk factor. The DQB1*0301 allele may have a protective role in the pathogenesis of idiopathic PSHL. These results suggest that the specific HLA class II gene products may confer susceptibility or resistance to idiopathic PSHL.
- Published
- 1996
- Full Text
- View/download PDF
44. Guinea pig inner ear antigens: extraction and application to the study of human autoimmune inner ear disease.
- Author
-
Cao MY, Deggouj N, Gersdorff M, and Tomasi JP
- Subjects
- Animals, Electrophoresis, Polyacrylamide Gel, Female, Guinea Pigs, Humans, Immunoblotting, Male, Meniere Disease immunology, Otosclerosis immunology, Antigens analysis, Autoimmune Diseases immunology, Ear Diseases immunology, Ear, Inner immunology
- Abstract
In this study, the authors attempted to develop a method of extracting guinea pig inner ear antigens for otoimmunological research, and to investigate the distribution of the antigens in the various structures of the inner ear. The antigens were extracted either from the entire or from various parts of the guinea pig inner ear. These antigens were separated on sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Western blot techniques were used to test sera from patients with inner ear disease against guinea pig inner ear protein extracts. It found that the various molecular weight antigens in the inner ear were associated with the different structures of the inner ear. The sera of 37.5% (N = 80) of patients reacted with two bands (30 and 58 kd) of the guinea pig inner ear immunoblots. The 58 kd band was not specific to the inner ear, but instead was also found in the immunoblots of other guinea pig tissues (brain, lung, and liver). This study suggests that the various antigens of interest could be further extracted and purified from the corresponding locations of the inner ear.
- Published
- 1996
- Full Text
- View/download PDF
45. [Total pancreatectomy after resection of the portal vein for solid and papillary tumor of the pancreas (clinical case)].
- Author
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Avisse C, Flament JB, Cailliez-Tomasi JP, Adam VL, Diebold MD, Plet H, Polarczyk Z, and Delattre JF
- Subjects
- Humans, Male, Pancreatectomy, Portal Vein surgery, Carcinoma, Papillary surgery, Pancreatic Neoplasms surgery
- Published
- 1996
46. Detection of inner ear disease autoantibodies by immunoblotting.
- Author
-
Cao MY, Gersdorff M, Deggouj N, Warny M, and Tomasi JP
- Subjects
- Adolescent, Adult, Aged, Animals, Antigens chemistry, Antigens isolation & purification, Blotting, Western, Case-Control Studies, Ear, Inner immunology, Female, Guinea Pigs, Hearing Loss, Sensorineural immunology, Hearing Loss, Sudden immunology, Humans, Male, Meniere Disease immunology, Middle Aged, Molecular Weight, Otosclerosis immunology, Proteins chemistry, Proteins immunology, Proteins isolation & purification, Autoantibodies blood, Labyrinth Diseases immunology
- Abstract
To define further the character of autoantibodies against the inner ear in patients with inner ear disease, Autoantibodies in sera from 82 patients with inner ear disease were investigated by immunoblotting. The inner ear antigens were extracted from Hartley guinea pigs. Brain, kidney, lung, heart and liver extracts were also prepared. Antibodies against the inner ear were found in 32 of 82 (39%) patients with inner ear disease. These sera reacted with the 30 and 58 kDa bands of the inner ear extracts. The 30 kDa band was detected in sera from patients with various inner ear diseases, while the 58 kDa band reacted with sera of patients with idiopathic progressive sensorineural hearing loss. Only two of the 52 normal control sera had a very faint band at 30 kDa. Sixteen of 32 positive sera were then used to probe Western blots of the brain, kidney, lung heart and liver extracts. The 58 kDa band was also found in the protein extracts of the brain, the lung, and the liver. On the other hand, preliminary purification of the 30 and 58 kDa proteins from the inner ear extracts were achieved by anion exchange chromatography. These results show that antibodies in sera from patients with inner ear disease reacted with at least two polypeptide bands (30 and 58 kDa) of guinea pig inner ear extracts, and the 58 kDa antigenic epitope was not cochlea specific.
- Published
- 1995
- Full Text
- View/download PDF
47. [Leiomyosarcoma of the inferior vena cava. Apropos of a new case].
- Author
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Avisse C, Plet H, Cailliez-Tomasi JP, Clement C, Deville J, Patey M, Pluot M, and Flament JB
- Subjects
- Blood Vessel Prosthesis, Fatal Outcome, Female, Humans, Leiomyosarcoma diagnostic imaging, Leiomyosarcoma pathology, Middle Aged, Neoplasm Metastasis, Retroperitoneal Neoplasms diagnostic imaging, Retroperitoneal Neoplasms pathology, Tomography, X-Ray Computed, Vena Cava, Inferior diagnostic imaging, Vena Cava, Inferior pathology, Leiomyosarcoma surgery, Retroperitoneal Neoplasms surgery, Vena Cava, Inferior surgery
- Abstract
A leiomyosarcoma was diagnosed in the mid portion of the inter-renal inferior vena cava. Survival was long (6 years) but required 14 reoperations to remove metastases. During the first operation, vascular integrity was reestablished with a cava-cava prosthesis after exeresis of the upstream cava without removal of the left renal vein ostium. The right renal vein was sectioned and directly implanted onto the prosthesis. The pathology report confirmed the diagnosis of leiomyosarcoma with multiple metastases presenting cytonuclear anomalies which worsened with time.
- Published
- 1995
48. The localization and specificity of guinea pig inner ear antigenic epitopes.
- Author
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Cao MY, Gersdorff M, Deggouj N, and Tomasi JP
- Subjects
- Animals, Basilar Membrane immunology, Brain immunology, Electrophoresis, Polyacrylamide Gel, Guinea Pigs, Humans, Immunoblotting, Liver immunology, Lung immunology, Molecular Weight, Organ Specificity, Organ of Corti immunology, Spiral Ganglion immunology, Stria Vascularis immunology, Vestibulocochlear Nerve immunology, Autoimmune Diseases immunology, Cochlea immunology, Ear, Inner immunology, Epitopes analysis, Hearing Loss, Sensorineural immunology
- Abstract
In this study, we investigated the relative localization of some antigenic epitopes in the inner ear. The inner ear protein antigens were extracted from various parts of the guinea pig inner ear. Brain, kidney, lung, heart and liver extracts were also obtained. We found by SDS-polyacrylamide gel electrophoresis that total inner ear extracts separated into three high concentration polypeptide bands with molecular weights of approximately 30, 42, 58 kd and three low density bands of 20, 25 and 35 kd. The 30 kd band was found mainly in the extract of the spiral ganglion and the acoustic nerve in the modiolus. The 42 and 58 kd bands were detected in the extract of the spiral ligament and the stria vascularis. The Organ of Corti and the basilar membrane extract gave rise to three bands of 30, 42 and 58 kd. Twenty-eight of the 75 sera from patients with inner ear disease reacted with the 30 and 58 kd bands of the inner ear protein extracts by immunoblotting. Sixteen of these 28 positive sera were then used to probe immunoblots of the brain, kidney, lung, heart and liver extracts. The 58 kd band was also found in protein extracts of the brain, the lung and the liver. This study suggests that the 30 kd antigenic epitope may be mainly related to the acoustic nerve and that the 58 kd antigenic epitope is not cochlear specific.
- Published
- 1995
- Full Text
- View/download PDF
49. Preformed antibody and complement rebound after plasma exchange: analysis of immunoglobulin isotypes and effect of splenectomy.
- Author
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Reding R, Soares M, Baranski A, Nisol F, Tomasi JP, Jamart J, Barker A, Latinne D, Lambotte L, and Bazin H
- Subjects
- Animals, Complement Hemolytic Activity Assay, Guinea Pigs, Isoantigens immunology, Male, Mice, Rats, Rats, Inbred Strains, Transplantation, Heterologous immunology, Antibody Formation immunology, Complement System Proteins biosynthesis, Immunoglobulin Isotypes biosynthesis, Plasma Exchange, Splenectomy
- Abstract
Unlabelled: Splenectomy (Sx) has been proposed to attenuate post-PE (plasma exchange) rebound of isoagglutinins and xenogenic (XG) antibody (Ab) in both ABO-incompatible allografts and discordant xenografts. This study analyses the qualitative nature and kinetics of serum immunoglobulins as well as complement resynthesis after PE in sham-operated (PE) and splenectomized (PE+Sx) syngeneic LOU/C rats; non-PE sham-operated or splenectomized animals were used as controls. PE was performed in unanesthetized, unheparinized rats. Immunoglobulin isotypes and subclasses (IgM, IgG1, IgG2 alpha, IgG2b) of total circulating Ab were measured pre-PE and up to 21 days post-PE, using ELISA (enzyme-linked immunosorbent assay) and specific mouse antirat monoclonal Ab. Antiguinea-pig (GP) XG Ab (IgM, IgG2a) serum levels were measured using cellular ELISA with cultured GP endothelial cells as targets. Sx alone significantly reduced XG IgM serum levels (p < 0.0001). Maximal rebound of total and XG IgM was observed on day 3 post-PE, reaching 674% and 187% of the pre-PE levels, respectively; these overshoots were entirely suppressed by Sx (p < 0.005 for total IgM; p < 0.0001 for XG IgM). Total IgG2a, IgG2b and IgG1 as well as XG IgG2a serum levels did not show significant overshoot post-PE. The activity of the complement classical pathway (mean +/- SD), assessed by CH50, was decreased at 51 +/- 19% of basal value 15 minutes after PE, and had returned to baseline level by day 2 post-PE with or without Sx., In Conclusion: (1) Six alone significantly reduced XG IgM serum levels; (2) early post-PE Ab rebound was mainly observed for IgM; (3) both total and XG IgM rebound was inhibited by Sx. This suggests that Sx probably removes a significant proportion of IgM producing cells undergoing post-PE stimulation. These data provide a rationale for combining PE with Sx in ABO-incompatible and discordant XG transplantation.
- Published
- 1994
- Full Text
- View/download PDF
50. Effect of plasmapheresis and splenectomy on parameters involved in vascular rejection of discordant xenografts in the swine to baboon model.
- Author
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Besse T, Duck L, Latinne D, Gianello P, Squifflet JP, Lievens M, Tomasi JP, Lavenne-Pardonge EM, Lambotte L, and Alexandre GP
- Subjects
- Animals, Blood Coagulation, Complement C3 analysis, Complement C4 analysis, Complement Pathway, Classical, Graft Rejection prevention & control, Humans, Immunoglobulin M blood, Papio, Platelet Count, Serum Albumin immunology, Swine, Graft Rejection immunology, Immunosuppression Therapy methods, Plasmapheresis, Splenectomy, Transplantation, Heterologous immunology
- Published
- 1994
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