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17 results on '"Tomaiuolo, Sara"'

1. The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Author

Claus, Clothilde, Slavin, Moriya, Ansseau, Eugénie, Lancelot, Céline, Bah, Karimatou, Lassche, Saskia, Fiévet, Manon, Greco, Anna, Tomaiuolo, Sara, Tassin, Alexandra, Dudome, Virginie, Kusters, Benno, Declèves, Anne-Emilie, Laoudj-Chenivesse, Dalila, van Engelen, Baziel G. M., Nonclercq, Denis, Belayew, Alexandra, Kalisman, Nir, and Coppée, Frédérique

Published
2023
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3. Additional file 3 of The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Author

Claus, Clothilde, Slavin, Moriya, Ansseau, Eugénie, Lancelot, Céline, Bah, Karimatou, Lassche, Saskia, Fiévet, Manon, Greco, Anna, Tomaiuolo, Sara, Tassin, Alexandra, Dudome, Virginie, Kusters, Benno, Declèves, Anne-Emilie, Laoudj-Chenivesse, Dalila, van Engelen, Baziel G. M., Nonclercq, Denis, Belayew, Alexandra, Kalisman, Nir, and Coppée, Frédérique

Abstract

Additional file 3: Figure S2. Validation of the rat anti-DUX4c serum.

Published
2023
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4. Additional file 10 of The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Author

Claus, Clothilde, Slavin, Moriya, Ansseau, Eugénie, Lancelot, Céline, Bah, Karimatou, Lassche, Saskia, Fiévet, Manon, Greco, Anna, Tomaiuolo, Sara, Tassin, Alexandra, Dudome, Virginie, Kusters, Benno, Declèves, Anne-Emilie, Laoudj-Chenivesse, Dalila, van Engelen, Baziel G. M., Nonclercq, Denis, Belayew, Alexandra, Kalisman, Nir, and Coppée, Frédérique

Abstract

Additional file 10: Figure S9. DUX4c co-detection with MYOD or CD56 as myogenic cell markers, and CD56 immunodetection pattern in FSHD muscle sections.

Published
2023
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5. Additional file 7 of The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Author

Claus, Clothilde, Slavin, Moriya, Ansseau, Eugénie, Lancelot, Céline, Bah, Karimatou, Lassche, Saskia, Fiévet, Manon, Greco, Anna, Tomaiuolo, Sara, Tassin, Alexandra, Dudome, Virginie, Kusters, Benno, Declèves, Anne-Emilie, Laoudj-Chenivesse, Dalila, van Engelen, Baziel G. M., Nonclercq, Denis, Belayew, Alexandra, Kalisman, Nir, and Coppée, Frédérique

Abstract

Additional file 7: Figure S6. DUX4c and laminin-α2 detection by co-immunofluorescence in FSHD muscle sections.

Published
2023
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6. Additional file 2 of The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Author

Claus, Clothilde, Slavin, Moriya, Ansseau, Eugénie, Lancelot, Céline, Bah, Karimatou, Lassche, Saskia, Fiévet, Manon, Greco, Anna, Tomaiuolo, Sara, Tassin, Alexandra, Dudome, Virginie, Kusters, Benno, Declèves, Anne-Emilie, Laoudj-Chenivesse, Dalila, van Engelen, Baziel G. M., Nonclercq, Denis, Belayew, Alexandra, Kalisman, Nir, and Coppée, Frédérique

Abstract

Additional file 2: Figure S1. DUX4C mRNAs and protein in muscle cells.

Published
2023
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7. Additional file 8 of The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Author

Claus, Clothilde, Slavin, Moriya, Ansseau, Eugénie, Lancelot, Céline, Bah, Karimatou, Lassche, Saskia, Fiévet, Manon, Greco, Anna, Tomaiuolo, Sara, Tassin, Alexandra, Dudome, Virginie, Kusters, Benno, Declèves, Anne-Emilie, Laoudj-Chenivesse, Dalila, van Engelen, Baziel G. M., Nonclercq, Denis, Belayew, Alexandra, Kalisman, Nir, and Coppée, Frédérique

Abstract

Additional file 8: Figure S7. DUX4c-desmin co-detection by immunofluorescence in FSHD muscles.

Published
2023
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8. Additional file 9 of The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Author

Claus, Clothilde, Slavin, Moriya, Ansseau, Eugénie, Lancelot, Céline, Bah, Karimatou, Lassche, Saskia, Fiévet, Manon, Greco, Anna, Tomaiuolo, Sara, Tassin, Alexandra, Dudome, Virginie, Kusters, Benno, Declèves, Anne-Emilie, Laoudj-Chenivesse, Dalila, van Engelen, Baziel G. M., Nonclercq, Denis, Belayew, Alexandra, Kalisman, Nir, and Coppée, Frédérique

Abstract

Additional file 9: Figure S8. DUX4c is immunodetected in regenerating myofibers.

Published
2023
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9. Additional file 6 of The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Author

Claus, Clothilde, Slavin, Moriya, Ansseau, Eugénie, Lancelot, Céline, Bah, Karimatou, Lassche, Saskia, Fiévet, Manon, Greco, Anna, Tomaiuolo, Sara, Tassin, Alexandra, Dudome, Virginie, Kusters, Benno, Declèves, Anne-Emilie, Laoudj-Chenivesse, Dalila, van Engelen, Baziel G. M., Nonclercq, Denis, Belayew, Alexandra, Kalisman, Nir, and Coppée, Frédérique

Abstract

Additional file 6: Figure S5. DUX4c detection by immunohistochemistry in muscle sections and histological alterations in FSHD muscles.

Published
2023
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10. Additional file 4 of The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Author

Claus, Clothilde, Slavin, Moriya, Ansseau, Eugénie, Lancelot, Céline, Bah, Karimatou, Lassche, Saskia, Fiévet, Manon, Greco, Anna, Tomaiuolo, Sara, Tassin, Alexandra, Dudome, Virginie, Kusters, Benno, Declèves, Anne-Emilie, Laoudj-Chenivesse, Dalila, van Engelen, Baziel G. M., Nonclercq, Denis, Belayew, Alexandra, Kalisman, Nir, and Coppée, Frédérique

Abstract

Additional file 4: Figure S3. Time-course of DUX4c expression in primary FSHD muscle cells.

Published
2023
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11. Additional file 5 of The double homeodomain protein DUX4c is associated with regenerating muscle fibers and RNA-binding proteins

Author

Claus, Clothilde, Slavin, Moriya, Ansseau, Eugénie, Lancelot, Céline, Bah, Karimatou, Lassche, Saskia, Fiévet, Manon, Greco, Anna, Tomaiuolo, Sara, Tassin, Alexandra, Dudome, Virginie, Kusters, Benno, Declèves, Anne-Emilie, Laoudj-Chenivesse, Dalila, van Engelen, Baziel G. M., Nonclercq, Denis, Belayew, Alexandra, Kalisman, Nir, and Coppée, Frédérique

Abstract

Additional file 5: Figure S4. DUX4c immunofluorescence intensity variation during primary myoblast differentiation time-course.

Published
2023
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13. QuilA® adjuvanted Coxevac® sustains Th1-CD8+-type immunity and increases protection in Coxiella burnetii-challenged goats.

Author

Tomaiuolo, Sara, Jansen, Wiebke, Soares Martins, Susana, Devriendt, Bert, Cox, Eric, and Mori, Marcella

Subjects
KILLER cells, Q fever, GOATS, VETERINARY vaccines, IMMUNITY, MATERNALLY acquired immunity
Abstract

Coxevac® is the EMA-approved veterinary vaccine for the protection of cattle and goats against Q fever, a zoonotic bacterial disease due to Coxiella burnetii. Since Coxevac® reduces bacterial shedding and clinical symptoms but does not prevent infection, novel, ready-to-use vaccine formulations are needed to increase its immunogenicity. Here, a goat vaccination-challenge model was used to evaluate the impact of the commercially available saponin-based QuilA® adjuvant on Coxevac® immunity. Upon challenge, the QuilA®-Coxevac® group showed a stronger immune response reflected in a higher magnitude of total IgG and an increase in circulating and splenic CD8+ T-cells compared to the Coxevac® and challenged-control groups. The QuilA®-Coxevac® group was characterized by a targeted Th1-type response (IFNγ, IP10) associated with increased transcripts of CD8+ and NK cells in spleens and γδ T cells in bronchial lymph nodes. Coxevac® vaccinated animals presented an intermediate expression of Th1-related genes, while the challenged-control group showed an immune response characterized by pro-inflammatory (IL1β, TNFα, IL12), Th2 (IL4 and IL13), Th17 (IL17A) and other immunoregulatory cytokines (IL6, IL10). An intriguing role was observed for γδ T cells, which were of TBX21- and SOX4-types in the QuilA®-Coxevac® and challenged control group, respectively. Overall, the addition of QuilA® resulted in a sustained Th1-type activation associated with an increased vaccine-induced bacterial clearance of 33.3% as compared to Coxevac® only. QuilA® could be proposed as a readily-applied veterinary solution to improve Coxevac® efficacy against C. burnetii infection in field settings. [ABSTRACT FROM AUTHOR]

Published
2023
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14. (Un)expected Similarity of the Temporary Adhesive Systems of Marine, Brackish, and Freshwater Flatworms

Author

Bertemes, Philip, Pjeta, Robert, Wunderer, Julia, Grosbusch, Alexandra L., Lengerer, Birgit, Grüner, Kevin, Knapp, Magdalena, Mertens, Birte, Andresen, Nikolas, Hess, Michael W., Tomaiuolo, Sara, Zankel, Armin, Holzer, Patrik, Salvenmoser, Willi, Egger, Bernhard, and Ladurner, Peter

Subjects
in situ hybridisation, Macrostomum, QH301-705.5, Animal Structures, Fresh Water, non-permanent adhesion, aquatic, Helminth Proteins, Article, Chemistry, RNA interference, duo-gland adhesive system, Biomimetic Materials, Platyhelminths, Adhesives, Animals, Seawater, Biology (General), glue, QD1-999
Abstract

Many free-living flatworms have evolved a temporary adhesion system, which allows them to quickly attach to and release from diverse substrates. In the marine Macrostomum lignano, the morphology of the adhesive system and the adhesion-related proteins have been characterised. However, little is known about how temporary adhesion is performed in other aquatic environments. Here, we performed a 3D reconstruction of the M. lignano adhesive organ and compared it to the morphology of five selected Macrostomum, representing two marine, one brackish, and two freshwater species. We compared the protein domains of the two adhesive proteins, as well as an anchor cell-specific intermediate filament. We analysed the gene expression of these proteins by in situ hybridisation and performed functional knockdowns with RNA interference. Remarkably, there are almost no differences in terms of morphology, protein regions, and gene expression based on marine, brackish, and freshwater habitats. This implies that glue components produced by macrostomids are conserved among species, and this set of two-component glue functions from low to high salinity. These findings could contribute to the development of novel reversible biomimetic glues that work in all wet environments and could have applications in drug delivery systems, tissue adhesives, or wound dressings.

Published
2021

15. Coxiella burnetii Shedding in Milk and Molecular Typing of Strains Infecting Dairy Cows in Greece

Author

Kalaitzakis, Emmanouil, Fancello, Tiziano, Simons, Xavier, Chaligiannis, Ilias, Tomaiuolo, Sara, Andreopoulou, Marianna, Petrone, Debora, Papapostolou, Aikaterini, Giadinis, Nektarios D., Panousis, Nikolaos, Mori, Marcella, Kalaitzakis, Emmanouil, Fancello, Tiziano, Simons, Xavier, Chaligiannis, Ilias, Tomaiuolo, Sara, Andreopoulou, Marianna, Petrone, Debora, Papapostolou, Aikaterini, Giadinis, Nektarios D., Panousis, Nikolaos, and Mori, Marcella

Abstract

Ruminants are considered the commonest animal reservoir for human infection of Coxiella burnetii, the Q fever causative agent. Considering the recently described importance of human Q fever in Greece, we aimed at providing the first comprehensive direct evidence of C. burnetii in dairy cows in Greece, including the genetic characterization of strains. The 462 examined dairy farms represented all geographical areas of Greece. One bulk tank milk sample was collected from every farm and tested for the presence of C. burnetii. Molecular genotyping of strains, performed directly on samples, revealed the existence of two separate clades characterized by single nucleotide polymorphism (SNP) genotypes of type 1 and type 2. The two clades were clearly distinguished in multiple locus variable-number tandem repeat analysis (MLVA) by two discriminative loci: MS30 and MS28. Whereas MLVA profiles of SNP-type 2 clade were closely related to strains described in other European cattle populations, the MLVA profile observed within the SNP type 1 clade highlighted a peculiar genetic signature for Greece, related to genotypes found in sheep and goats in Europe. The shedding of C. burnetii bearing this genotype might have yet undefined human epidemiological consequences. Surveillance of the genetic distribution of C. burnetii from different sources is needed to fully understand the epidemiology of Q fever in Greece.

Published
2021

16. Coxiella burnetii Shedding in Milk and Molecular Typing of Strains Infecting Dairy Cows in Greece

Author

Kalaitzakis, Emmanouil, primary, Fancello, Tiziano, additional, Simons, Xavier, additional, Chaligiannis, Ilias, additional, Tomaiuolo, Sara, additional, Andreopoulou, Marianna, additional, Petrone, Debora, additional, Papapostolou, Aikaterini, additional, Giadinis, Nektarios D., additional, Panousis, Nikolaos, additional, and Mori, Marcella, additional

Published
2021
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