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158 results on '"Tokuda, Kazuhiro"'

8. Down-regulating Effect of a Standardized Extract of CulturedLentinula edodes myceliaon Cortactin in Prostate Cancer Cells Is Dependent on Malignant Potential

Author

YAMASHITA, SHIN-NOSUKE, primary, TANAKA, YOSHIATSU, additional, KITAGAWA, TAKAO, additional, BARON, BYRON, additional, TOKUDA, KAZUHIRO, additional, PAUDEL, DURGA, additional, NAKAGAWA, KOJI, additional, OHTA, TOHRU, additional, HAMADA, JUN-ICHI, additional, KOBAYASHI, MASANOBU, additional, NAGAYASU, HIROKI, additional, and KURAMITSU, YASUHIRO, additional

Published
2023
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10. Proteomic Analysis of Hepatocellular Carcinoma Tissues With Encapsulation Shows Up-regulation of Leucine Aminopeptidase 3 and Phosphoenolpyruvate Carboxykinase 2

Author

Kuhara, Keisuke, Tokuda, Kazuhiro, Kitagawa, Takao, Baron, Byron, Tokunaga, Masayuki, Sakamoto, Kazuhiko, Nagano, Hiroaki, Nakamura, Kazuyuki, Kobayashi, Masanobu, Nagayasu, Hiroki, and Kuramitsu, Yasuhiro

Subjects
Male, Proteomics, Cancer Research, Carcinoma, Hepatocellular, Biology, Biochemistry, Metastasis, Leucyl Aminopeptidase, Leucine aminopeptidase, Western blot, PCK2, Genetics, medicine, Humans, neoplasms, Molecular Biology, medicine.diagnostic_test, Liver Neoplasms, Cancer, Capsule, Prognosis, medicine.disease, digestive system diseases, Up-Regulation, Liver -- Cancer, Hepatocellular carcinoma, Cancer research, Female, Human cell culture, Phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate Carboxykinase (ATP), Research Article
Abstract

Background/Aim: Cancer is the most fatal disease worldwide whose most lethal characteristics are invasion and metastasis. Hepatocellular carcinoma (HCC) is one of the most fatal cancers worldwide. HCC often shows encapsulation, which is related to better prognosis. In this study, proteomic analysis of HCC tissues with and without encapsulation was performed, in order to elucidate the factors which play important roles in encapsulation., Materials and Methods: Five HCC tissues surrounded by a capsule and five HCC tissues which broke the capsule were obtained from patients diagnosed with HCC who underwent surgical liver resection. Protein samples from these tissues were separated by two-dimensional gel electrophoresis (2-DE), and the protein spots whose expression was different between encapsulated and non-encapsulated HCC tissues were identified through gel imaging analysis software. The selected protein spots were analyzed and identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS)., Results: Two-DE analysis showed 14 spots whose expression was different between encapsulated and non-encapsulated HCC tissues. Of these, 9 were up-regulated and 5 were downregulated in HCC tissues without encapsulation. The validation by Western blot confirmed that leucine aminopeptidase 3 (LAP3) and phosphoenolpyruvate carboxykinase mitochondrial (PCK2) were up-regulated significantly in HCC tissues with a capsule, compared to HCC tissues that broke the capsule., Conclusion: These findings suggest that LAP3 and PCK2 could be factors responsible for the maintenance of encapsulation in HCC tissues., peer-reviewed

Published
2021
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16. Correction: Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis

Author

Uchi, Sho-Hei, primary, Yanai, Ryoji, additional, Kobayashi, Masaaki, additional, Hatano, Makoto, additional, Kobayashi, Yuka, additional, Yamashiro, Chiemi, additional, Nagai, Tomohiko, additional, Tokuda, Kazuhiro, additional, Connor, Kip M., additional, Sonoda, Koh-Hei, additional, and Kimura, Kazuhiro, additional

Published
2020
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24. Up‐regulation of the pentose phosphate pathway and HIF‐1α expression during neural progenitor cell induction following glutamate treatment in rat ex vivo retina.

Author

Tokuda, Kazuhiro, Baron, Byron, Yamashiro, Chiemi, Kuramitsu, Yasuhiro, Kitagawa, Takao, Kobayashi, Masaaki, Sonoda, Koh‐Hei, and Kimura, Kazuhiro

Subjects
*PENTOSE phosphate pathway, *NICOTINAMIDE adenine dinucleotide phosphate, *HYPOXIA-inducible factor 1, *RETINA, *GLUCOSE-6-phosphate dehydrogenase, *DNA synthesis, *PYRUVATE kinase, *PROGENITOR cells
Abstract

The metabolic state influences the regulation of neural stem/progenitor cells. The pentose phosphate pathway (PPP), an alternative metabolic pathway that operates parallel to glycolysis, not only provides key intermediates for biosynthetic reactions but also controls the fate of neural stem/progenitor cells. We have previously shown that glutamate application leads to the induction of neural progenitor cells in mature ex vivo rat retina. In this study, we investigated whether regulation of the PPP might be changed following glutamate treatment of the retina. Immunoblot analysis revealed that the amount of glucose‐6‐phosphate dehydrogenase (G6PD), the rate‐limiting enzyme of the PPP as well as that of 6‐phosphogluconate dehydrogenase (6PGD), another enzyme in this pathway, increased in the glutamate‐treated retina. Consistent with the fact that both these enzymes generate reduced nicotinamide adenine dinucleotide phosphate (NADPH), the amount of NAPDH in the treated retina was significantly higher compared with that in the untreated retina. We also found that both DNA synthesis as well as the expression of fatty acid synthase (FASN) increased significantly in the glutamate‐treated retina. Furthermore, hypoxia‐inducible factor 1‐α (HIF‐1α), a positive transcriptional regulator of PPP enzymes, was up‐regulated at both messenger RNA (mRNA) and protein levels. Finally, we found the interaction of HIF‐1α with the M2 isozyme of pyruvate kinase (PKM2), with this interaction having been shown to contribute to a positive feedback loop in the control of glycolysis. Our results thus show that specific metabolic change in the PPP occurs in the process of neural progenitor cell induction in the mature rat retina. [ABSTRACT FROM AUTHOR]

Published
2020
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25. CUB Domain-containing Protein 1 (CDCP1) Is Down-regulated by Active Hexose-correlated Compound in Human Pancreatic Cancer Cells

Author

KUHARA, KEISUKE, primary, TOKUDA, KAZUHIRO, additional, KITAGAWA, TAKAO, additional, BARON, BYRON, additional, TOKUNAGA, MASAYUKI, additional, HARADA, KOJI, additional, TERASAKI, MASARU, additional, UEHARA, OSAMU, additional, OHTA, TOHRU, additional, TAKAI, RIE, additional, HAMADA, JUN-ICHI, additional, KOBAYASHI, MASANOBU, additional, SHIMO, TSUYOSHI, additional, NAGAYASU, HIROKI, additional, and KURAMITSU, YASUHIRO, additional

Published
2018
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48. Ethanol Enhances Neurosteroidogenesis in Hippocampal Pyramidal Neurons by Paradoxical NMDA Receptor Activation.

Author

Tokuda, Kazuhiro, Izumi, Yukitoshi, and Zorumski, Charles F.

Subjects
*IMMUNOGLOBULINS, *LABORATORY rats, *ETHANOL, *ENZYMES, *FINASTERIDE, *CHEMICAL inhibitors
Abstract

Using an antibody against 5α-reduced neurosteroids, predominantly allopregnanolone, we found that immunostaining in the CA1 region of rat hippocampal slices was confined to pyramidal neurons. This neurosteroid staining was increased following 15 min administration of 60 mM but not 20 mM ethanol, and the enhancement was blocked by finasteride and dutasteride, selective inhibitors of 5α-reductase, a key enzyme required for allopregnanolone synthesis. Consistent with a prior report indicating that N-methyl-D-aspartate (NMDA) receptor (NMDAR) activation can promote steroid production, we observed that D-2-amino-5-phosphonovalerate (APV), a competitive NMDAR antagonist, blocked the effects of 60 mM ethanol on staining. We previously reported that 60 mM ethanol inhibits the induction of long-term potentiation (LTP), a cellular model for memory formation, in the CA1 region. In the present study, LTP inhibition by 60m Methanol was also overcome by both the 5α-reductase inhibitors and by APV. Furthermore, the effects of ethanol on neurosteroid production and LTP were mimicked by a low concentration of NMDA (1 μM), and the ability of NMDA to inhibit LTP and to enhance neurosteroid staining was reversed by finasteride and dutasteride, as well as by APV. These results indicate that ethanol paradoxically enhances GABAergic neurosteroid production by activation of unblocked NMDARs and that acute LTP inhibition by ethanol represents a form of NMDAR-mediated metaplasticity. [ABSTRACT FROM AUTHOR]

Published
2011
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49. Long-term potentiation inhibition by low-level N-methyl- D-aspartate receptor activation involves calcineurin, nitric oxide, and p38 mitogen-activated protein kinase.

Author

Izumi, Yukitoshi, Tokuda, Kazuhiro, and Zorumski, Charles F.

Abstract

Low-level activation of N-methyl- d-aspartate receptors (NMDARs) results in a decrease in the ability of tetanic stimulation to induce long-term potentiation (LTP). This NMDAR-mediated LTP inhibition is observed with low micromolar concentrations of NMDA or chelation of ambient extracellular zinc. In rat hippocampal slices, we examined whether LTP inhibition by 1 μM NMDA and zinc chelation share common mechanisms. We found that both forms of LTP inhibition involve nitric oxide (NO) synthase (NOS) and calcineurin. Furthermore, both forms of LTP inhibition are overcome by block of p38 mitogen-activated protein kinase (MAPK), but not by inhibition of extracellular signal-regulated kinase 1/2 or c-Jun-N-terminal kinase. A p38 antagonist also overcame the block of LTP by sodium nitroprusside, an agent that releases NO, suggesting that NO release occurs upstream of MAPK activation. Despite the involvement of p38 MAPK in NMDAR-mediated LTP inhibition, p38 antagonism did not enhance LTP induction in response to weak tetanic stimulation under baseline conditions. These results indicate that p38 MAPK is part of a complex NMDAR-driven signaling pathway involving calcineurin and NO that helps to regulate synaptic plasticity in the CA1 region. © 2007 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]

Published
2008
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