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Background With the recent emphasis on implementation of interprofessional education (IPE), simulation can be a successful and impactful pedagogy to train health profession learners. Simulation allows opportunities to practice decision-making and working in teams in a realistic and safe environment. Participants learn with and about other professions and have the chance to clarify roles within their team. Simulation-enhanced IPE can present challenges for faculty. This article seeks to provide insight into key aspects of interprofessional simulation to maximize its successful implementation. Methods Our team has identified key lessons learned in how we successfully implemented IPE using simulation. We have collectively overcome challenges and barriers as we have implemented simulation-based IPE at our university. Results To implement effective simulation-enhanced IPE, teams should consider an overall need assessment, curriculum integration, logistical plans, faculty development, a structured prebrief and debrief plan, and a process for evaluation. Conclusions Although IPE is an important component of training for future health care providers, simulation can be an effective strategy for implementation of interprofessional activities. However, it is essential to consider key aspects to ensure a quality and consistent experience for learners.

Background Despite a 2009 recommendation from the AMA that disaster medicine and public health response training should be implemented in medical schools, anywhere from 31% to 47% of medical education programs lack a formalized disaster medicine curriculum. A need for disaster medicine response training for University of Alabama medical students in an appropriately socially distanced format was identified during the COVID‐19 pandemic. Methods Our emergency medicine faculty in collaboration with the Federal Emergency Management Agency Center for Disaster Preparedness (FEMA CDP) created and implemented a novel virtual disaster medicine and pandemic response course for third year medical students at the University of Alabama at Birmingham. The course was administered via a teleconferencing platform (Zoom, Zoom Video Communications, Inc.) in the spring of 2020 to greater than 130 medical students. Results Using pre and post course surveys, we assessed a change in student confidence levels for their ability to explain topics covered in the course and their understanding of a chosen disaster. The students reported an average increase of 2.183 on a 5‐point scale, with a score of 5 representing “completely confident” and a score of 1 representing “not at all confident”. This course established the feasibility of a virtual instructor led training (VILT) format for disaster medicine education and provided a template for the delivery of over 300 courses to more than 4,000 first responders and medical professionals through the FEMA CDP. Conclusions Through collaboration with the FEMA CDP, our UAB faculty were able to successfully deliver a novel virtual disaster preparedness and response course. The course resulted in subjective improvement of students’ content understanding while also establishing the feasibility and effectiveness of a VILT format that could be readily applied to future courses in undergraduate medical education and beyond.

This paper examines the trends in electrical disturbances and how these trends influence decision-making. Funding for the smart grid from government grants has enabled almost $9.5 billion dollars worth of spending geared toward installing smart meters and self-healing and reconfigurable assets, to increase the resiliency and reliability of the electrical grid. Using Value of Service, we find that the cost of outage events has reduced during the deployment of the smart grid and generated positive net present value, reasonable payback periods, and a respectable rate of return.

Author(s): Robinson, Daniel W.; Anana, Michael; Edens, Mary A.; Kanter, Marc; Khandelwal, Sorabh; Shah, Kaushal; Peterson, Todd | Abstract: Introduction: Obstetrical emergencies are a high-risk yet infrequent occurrence in the emergency department. While U.S. emergency medicine (EM) residency graduates are required to perform 10 low-risk normal spontaneous vaginal deliveries, little is known about how residencies prepare residents to manage obstetrical emergencies. We sought to profile the current obstetrical training curricula through a survey of U.S. training programs.Methods: We sent a web-based survey covering the four most common obstetrical emergencies (pre-eclampsia/eclampsia, postpartum hemorrhage (PPH), shoulder dystocia, and breech presentation) through email invitations to all program directors (PD) of U.S. EM residency programs. The survey focused on curricular details as well as the comfort level of the PDs in the preparation of their graduating residents to treat obstetrical emergencies and normal vaginal deliveries.Results: Our survey had a 55% return rate (n=105/191). Of the residencies responding, 75% were in the academic setting, 20.2% community, 65% urban, and 29.8% suburban, and the obstetrical curricula were 2-4 weeks long occurring in post-graduate year one. The most common teaching method was didactics (84.1-98.1%), followed by oral cases for pre-eclampsia (48%) and PPH (37.2%), and homemade simulation for shoulder dystocia (37.5%) and breech delivery (33.3%). The PDs’ comfort about residency graduate skills was highest for normal spontaneous vaginal delivery, pre-eclampsia, and PPH. PDs were not as comfortable about their graduates’ skill in handling shoulder dystocia or breech delivery.Conclusion: Our survey found that PDs are less comfortable in their graduates’ ability to perform non-routine emergency obstetrical procedures.

Product line engineering and model based engineering are two powerful engineering approaches that each bring significant advantages to system engineering projects. This paper explores how three companies - Raytheon, General Dynamics, and General Motors - are combining these two paradigms in unique and innovative ways in very challenging application domains to achieve engineering goals of critical importance to them.

Chlamydia is a prevalent sexually transmitted disease that infects more than 100 million people worldwide. Although most individuals infected with Chlamydia trachomatis are initially asymptomatic, symptoms can arise if left undiagnosed. Long-term infection can result in debilitating conditions such as pelvic inflammatory disease, infertility, and blindness. Chlamydia infection, therefore, constitutes a significant public health threat, underscoring the need for a Chlamydia-specific vaccine. Chlamydia strains express a major outer-membrane protein (MOMP) that has been shown to be an effective vaccine antigen. However, approaches to produce a functional recombinant MOMP protein for vaccine development are limited by poor solubility, low yield, and protein misfolding. Here, we used an Escherichia coli-based cell-free system to express a MOMP protein from the mouse-specific species Chlamydia muridarum (MoPn-MOMP or mMOMP). The codon-optimized mMOMP gene was co-translated with Δ49apolipoprotein A1 (Δ49ApoA1), a truncated version of mouse ApoA1 in which the N-terminal 49 amino acids were removed. This co-translation process produced mMOMP supported within a telodendrimer nanolipoprotein particle (mMOMP–tNLP). The cell-free expressed mMOMP–tNLPs contain mMOMP multimers similar to the native MOMP protein. This cell-free process produced on average 1.5 mg of purified, water-soluble mMOMP–tNLP complex in a 1-ml cell-free reaction. The mMOMP–tNLP particle also accommodated the co-localization of CpG oligodeoxynucleotide 1826, a single-stranded synthetic DNA adjuvant, eliciting an enhanced humoral immune response in vaccinated mice. Using our mMOMP–tNLP formulation, we demonstrate a unique approach to solubilizing and administering membrane-bound proteins for future vaccine development. This method can be applied to other previously difficult-to-obtain antigens while maintaining full functionality and immunogenicity.

An aged circulatory environment can activate microglia, reduce neural precursor cell activity and impair cognition in mice. We hypothesized that brain endothelial cells (BECs) mediate at least some of these effects. We observe that BECs in the aged mouse hippocampus express an inflammatory transcriptional profile with focal upregulation of vascular cell adhesion molecule 1 (VCAM1), a protein that facilitates vascular-immune cell interactions. Concomitantly, levels of the shed, soluble form of VCAM1 are prominently increased in the plasma of aged humans and mice, and their plasma is sufficient to increase VCAM1 expression in cultured BECs and the hippocampi of young mice. Systemic administration of anti-VCAM1 antibody or genetic ablation of Vcam1 in BECs counteracts the detrimental effects of plasma from aged individuals on young brains and reverses aging aspects, including microglial reactivity and cognitive deficits, in the brains of aged mice. Together, these findings establish brain endothelial VCAM1 at the blood-brain barrier as a possible target to treat age-related neurodegeneration.

Since September 2001, NASA's In-Space Propulsion Technology (ISPT) program has been developing technologies for lowering the cost of planetary science missions. Recently completed is the high-temperature Advanced Material Bipropellant Rocket (AMBR) engine providing higher performance for lower cost. Two other cost saving technologies nearing completion are the NEXT ion thruster and the Aerocapture technology project. Under development are several technologies for low-cost sample return missions. These include a low-cost Hall-effect thruster (HIVHAC) which will be completed in 2011, light-weight propellant tanks, and a Multi-Mission Earth Entry Vehicle (MMEEV). This paper will discuss the status of the technology development, the cost savings or performance benefits, and applicability of these in-space propulsion technologies to NASA's future Discovery, and New Frontiers missions, as well as their relevance for sample return missions.

Outlaw motorcycle gangs (OMGs) are an iconic element of the criminal landscape in the United States, the country of their origin. Members of OMGs may present to the emergency department (ED) as a result of motor vehicle accidents or interpersonal violence. When one member of an OMG is injured, other members and associates are likely to arrive in the ED to support the injured member. The extant literature for ED personnel lacks an overview of the culture of OMGs, a culture that promotes the display of unique symbols and that holds certain paraphernalia as integral to an outlaw biker’s identity and pride. The objective of this manuscript is to discuss various aspects of the culture of OMGs so that ED personnel may better understand the mentality of the outlaw biker. Knowledge of their symbols, values, and hierarchy can be crucial to maintaining order in the ED when an injured outlaw biker presents to the ED. We used standard search engines to obtain reports from law enforcement agencies and studies in academic journals on OMGs. We present the observations of 1 author who has conducted ethnographic research on outlaw bikers since the 1980s.

Large genetic engineering projects require more cistrons and consequently more strong and reliable transcriptional terminators. We have measured the strengths of a library of terminators, including 227 that are annotated in Escherichia coli--90 of which we also tested in the reverse orientation--and 265 synthetic terminators. Within this library we found 39 strong terminators, yielding50-fold reduction in downstream expression, that have sufficient sequence diversity to reduce homologous recombination when used together in a design. We used these data to determine how the terminator sequence contributes to its strength. The dominant parameters were incorporated into a biophysical model that considers the role of the hairpin in the displacement of the U-tract from the DNA. The availability of many terminators of varying strength, as well as an understanding of the sequence dependence of their properties, will extend their usability in the forward design of synthetic cistrons.

Recombinant DNA technologies have had a fundamental impact on drug discovery. The continuous emergence of unique gene assembly techniques resulted in the generation of a variety of therapeutic reagents such as vaccines, cancer treatment molecules and regenerative medicine precursors. With the advent of synthetic biology there is a growing need for precise and concerted assembly of multiple DNA fragments of various sizes, including chromosomes. In this article, we summarize the highlights of the recombinant DNA technology since its inception in the early 1970s, emphasizing on the most recent advances, and underscoring their principles, advantages and shortcomings. Current and prior cloning trends are discussed in the context of sequence requirements and scars left behind. Our opinion is that despite the remarkable progress that has enabled the generation and manipulation of very large DNA sequences, a better understanding of the cell's natural circuits is needed in order to fully exploit the current state-of-the-art gene assembly technologies.

Managing ecosystems to maintain biodiversity may be one approach to ensuring their dynamic stability, productivity, and delivery of vital services. The applicability of this approach to industrial ecosystems that harness the metabolic activities of microbes has been proposed but has never been tested at relevant scales. We used a tag-sequencing approach with bacterial small subunit rRNA (16S) genes and eukaryotic internal transcribed spacer 2 (ITS2) to measuring the taxonomic composition and diversity of bacteria and eukaryotes in an open pond managed for bioenergy production by microalgae over a year. Periods of high eukaryotic diversity were associated with high and more-stable biomass productivity. In addition, bacterial diversity and eukaryotic diversity were inversely correlated over time, possibly due to their opposite responses to temperature. The results indicate that maintaining diverse communities may be essential to engineering stable and productive bioenergy ecosystems using microorganisms.

Structural and functional studies of membrane proteins have been severely hampered by difficulties in producing sufficient quantities of properly folded protein products. It is well established that cell-based expression of membrane proteins is generally problematic and frequently results in low yield, cell toxicity, protein aggregation and misfolding. Owing to its inherent open nature, cell-free protein expression has become a highly promising tool for the fast and efficient production of these difficult-to-express proteins. Here we review the most recent advances in this field, underscoring the potentials and weaknesses of the newly developed approaches and place specific emphasis on the use of nanolipoprotein particles (NLPs or nanodiscs).

Here we demonstrate rapid production of solubilized and functional membrane protein by simultaneous cell-free expression of an apolipoprotein and a membrane protein in the presence of lipids, leading to the self-assembly of membrane protein-containing nanolipoprotein particles (NLPs). NLPs have shown great promise as a biotechnology platform for solubilizing and characterizing membrane proteins. However, current approaches are limited because they require extensive efforts to express, purify, and solubilize the membrane protein prior to insertion into NLPs. By the simple addition of a few constituents to cell-free extracts, we can produce membrane proteins in NLPs with considerably less effort. For this approach an integral membrane protein and an apolipoprotein scaffold are encoded by two DNA plasmids introduced into cell-free extracts along with lipids. For this study reported here we used plasmids encoding the bacteriorhodopsin (bR) membrane apoprotein and scaffold protein Delta1-49 apolipoprotein A-I fragment (Delta49A1). Cell free co-expression of the proteins encoded by these plasmids, in the presence of the cofactor all-trans-retinal and dimyristoylphosphatidylcholine, resulted in production of functional bR as demonstrated by a 5-nm shift in the absorption spectra upon light adaptation and characteristic time-resolved FT infrared difference spectra for the bR --M transition. Importantly the functional bR was solubilized in discoidal bR.NLPs as determined by atomic force microscopy. A survey study of other membrane proteins co-expressed with Delta49A1 scaffold protein also showed significantly increased solubility of all of the membrane proteins, indicating that this approach may provide a general method for expressing membrane proteins enabling further studies.

Solar array technologies that can empower electric thrusters in direct drive mode may provide significant mission benefits by reducing power processing, system complexity, weight, and cost over conventional systems. In direct-drive systems the solar arrays will operate at high voltages and must perform safely in the surrounding plasma environment, with minimal loss of performance due to parasitic current collection. For example, current state-of-the-art Hail effect thrusters in the kilowatt class require applied voltages of 300 V or higher. Results from experiments and modeling of electron current collection at high bias voltages (300-500 V) are presented. The experiments employed two sample solar array coupon technologies. A hollow cathode was used to emulate the induced environment around the solar arrays far from the Hall thruster and in nearby regions populated by charge-exchange plasma (10 1 2 -10 1 3 m - 3 and 0.5-1 eV). The measurements show that tens to hundreds of seconds are required before the collected current relaxes to a quasi-steady value. Comparisons with results from numerical calculations suggest that changes of the secondary electron yield properties of the dielectric materials may account for the observed current collection trends.

Progressive development of microsatellite technologies has resulted in increased demand for lightweight electrical power subsystems including solar arrays. The use of thin film photovoltaics has been recognized as a key solution to meet the power needs. The lightweight cells can generate sufficient power and still meet critical mass requirements. Commercially available solar cells produced on lightweight substrates are being studied as an option to fulfill the power needs. The commercially available solar cells are relatively inexpensive and have a high payoff potential. Commercially available thin film solar cells are primarily being produced for terrestrial applications. The need to convert the solar cell from a terrestrial to a space compatible application is the primary challenge. Solar cell contacts, grids and interconnects need to be designed to be atomic oxygen resistant and withstand rapid thermal cycling environments. A mechanically robust solar cell interconnect is also required in order to withstand handling during fabrication and survive during launch. The need to produce the solar cell interconnects has been identified as a primary goal of the Powersphere program and is the topic of this paper. Details of the trade study leading to the final design involving the solar cell wrap around contact, flex blanket, welding process, and frame will be presented at the conference.

The Aerospace Corporation, NASA Glenn Research Center, Lockheed-Martin, and ILC Dover over the past two years have been engaged in developing a Multifunctional Inflatable Structure for the Powersphere Concept under contract with NASA (NAS3-01115). The Powersphere concept consists of a relatively large spherical solar array, which would be deployed from a microsatellite. The Powersphere structure and the deployment method was patented by the Aerospace Corporation (U.S. Patent Numbers 6,284,966 B 1 and 6,3 18,675). The work on this project has resulted in a number of technological innovations in the state of the art for integrating flexible thin-film solar cells with flex circuit harness technology and inflatable ultraviolet-light-rigidizable structures. The specific power, specific volume, for the Powersphere are presented in Figures 1 and 2 as a function of solar cell technology and efficiency. The Powersphere will enable microsatellite missions across NASA enterprises and DoD missions by providing ample electric power at an affordable cost. The Powersphere design provides attitude-independent electric power and thermal control for an enclosed microsatellite payload. The design is scalable, robust in high radiation environments and provides sufficient electric power to allow the use of electric propulsion. Electric propulsion enables precise positioning of microsatellites which is required for inspectors that would be deployed to inspect the International Space Station, Space Shuttle or large unmanned spacecraft. The Powersphere allows for efficient launch packaging versus deployed volume as shown in Figure 3.

The application of resonance light scattering (RLS) particles for high-sensitivity detection of DNA hybridization on cDNA microarrays is demonstrated. Arrays composed of approximately 2000 human genes ("targets") were hybridized with colabeled (Cy3 and biotin) human lung cDNA probes at concentrations ranging from 8.3 ng/microL to 16.7 pg/microL. After hybridization, the arrays were imaged using a fluorescence scanner. The arrays were then treated with 80-nm-diameter gold RLS Particles coated with anti-biotin antibodies and imaged in a white light, CCD-based imaging system. At low probe concentrations, significantly more genes were detected by RLS compared to labeling by Cy3. For example, for hybridizations with a probe concentration of 83.3 pg/microL, approximately 1150 positive genes were detected using RLS compared to approximately 110 positive genes detected with Cy3. In a differential gene expression experiment using human lung and leukemia RNA samples, similar differential expression profiles were obtained for labeling by RLS and fluorescence technologies. The use of RLS Particles is particularly attractive for detection and identification of low-abundance mRNAs and for those applications in which the amount of sample is limited.

ASM President Jeffery F. Miller traveled across the globe to represent the Society at the 53rd Annual Conference of the Association of Microbiologists of India (AMI), held at KIIT University, November 22–25 in Bhubaneswar, India. Miller’s visit was a testament to the growing collaboration between American and Indian microbiologists within ASM, as well as a call to action for both societies. The visit was highly publicized throughout the Indian scientific community in the months preceding the AMI Conference which drew over 2,400 attendees from the United States, Europe, Asia and across India. At the invitation of AMI President, Prof. L. Venkateswara Rao, Miller energized the conference during the opening ceremonies with inspiring remarks on the critical role scientific societies play in addressing today’s global challenges. He also stressed the need for international collaboration among individuals and institutions such as ASM and AMI. Miller then delivered the keynote lecture titled “Diversity-Generating Retroelements in Phage, Pathogens, and the Human Microbiome.” Prior to the meeting’s opening ceremonies, Miller joined ASM Ambassador to India, Rup Lal, University of Delhi, ASM Committee on Global Engagement (CGE) member, Sunil Lal, ICGEB, and over 100 participants in the ASM Workshop on Scientific Writing and Publishing. The 4-h workshop combined virtual lectures on best practices in developing strong scientific manuscripts with onsite activities and discussion led by Sunil Lal and Miller. As a benefit of membership, ASM members participated in the workshop for free and 88 new members joined the Society as a result of their attendance. Feedback from participants has been exceedingly positive and representatives from several Indian universities requested that the workshop be repeated at their institutions in 2013. Miller also chaired the ASM–AMI Joint Symposium on Genomics and Proteomics which included lectures by Rup Lal, “Genetics, Genomics and Metagenomics in the Aid of Developing a Bioremediation Technology for Hexachlorocyclohexane”; Jack Gilbert, Argonne National Labs on “The Earth Microbiome Project: A New Paradigm in Geospatial and Temporal Studies of Microbial Ecology,” and Rakesh Sharma, IGIB on “Functional Metagenomics Reveals Novel Genes Involved in Arsenic Resistance.” Finally, as the AMI President-elect, Rup Lal invited Miller and Sunil Lal to a formal dinner with leadership from throughout the Indian microbiology community to discuss ways ASM and AMI can continue to foster the collaborative scientific partnership. In 2012, ASM membership in India increased 149 % to 833 members. Follow-up discussions are already underway to ensure that the momentum gained during the President’s visit translates into positive symbiotic cooperation. “There is a palpable sense of excitement and optimism in the Indian microbiology community, due in part to a clear commitment at the national level to support research and education. ASM, in partnership with the AMI, has much to offer. The science at the meeting was excellent and the students, in particular, were an inspiration”, said Miller. ASM is grateful to AMI and KIIT University for the opportunity to share in this important scientific event, their enthusiasm in strengthening the ASM–AMI partnership, and the generous hospitality shown to the ASM President during his visit. ASM would also like to recognize the significant efforts of the Rup Lal, Sunil Lal and the AMI Conference Organizing Secretary, Mrutyunjay Suar, KIIT University, in coordinating ASM’s participation in the conference.

© The Author(s) 2014. Inspired by the developments of synthetic biology and the need for improved genetic tools to exploit cyanobacteria for the production of renewable bioproducts, we developed a versatile platform for the construction of broad-host-range vector systems. This platform includes the following features: (i) an efficient assembly strategy in which modules released from 3 to 4 donor plasmids or produced by polymerase chain reaction are assembled by isothermal assembly guided by short GC-rich overlap sequences. (ii) A growing library of molecular devices categorized in three major groups: (a) replication and chromosomal integration; (b) antibiotic resistance; (c) functional modules. These modules can be assembled in different combinations to construct a variety of autonomously replicating plasmids and suicide plasmids for gene knockout and knockin. (iii) A web service, the CYANO-VECTOR assembly portal, which was built to organize the various modules, facilitate the in silico construction of plasmids, and encourage the use of this system. This work also resulted in the construction of an improved broadhost-range replicon derived from RSF1010, which replicates in several phylogenetically distinct strains including a new experimental model strain Synechocystis sp. WHSyn, and the characterization of nine antibiotic cassettes, four reporter genes, four promoters, and a ribozyme-based insulator in several diverse cyanobacterial strains.

This article addresses weighting and partitioning, in complex reinforcement learning tasks, with the aim of facilitating learning. The article presents some ideas regarding weighting of multiple agents and extends them into partitioning an input/state space into multiple regions with differential weighting in these regions, to exploit differential characteristics of regions and differential characteristics of agents to reduce the learning complexity of agents (and their function approximators) and thus to facilitate the learning overall. It analyzes, in reinforcement learning tasks, different ways of partitioning a task and using agents selectively based on partitioning. Based on the analysis, some heuristic methods are described and experimentally tested. We find that some off-line heuristic methods perform the best, significantly better than single-agent models.

To deal with sequential decision tasks, we present a learning model CLARION, which is a hybrid connectionist model consisting of both localist and distributed representations, based on the two-level approach proposed in our earlier work (Artificial Intelligence, 75(2) (1995) 241–296). The model learns and utilizes procedural and declarative knowledge, tapping into the synergy of the two types of processes. It unifies neural, reinforcement, and symbolic methods to perform on-line, bottom-up learning. Experiments in various situations are reported that shed light on the working of the model.

Genetic circuits perform computational operations based on interactions between freely diffusing molecules within a cell. When transcription factors are combined to build a circuit, unintended interactions can disrupt its function. Here, we apply 'part mining' to build a library of 73 TetR-family repressors gleaned from prokaryotic genomes. The operators of a subset were determined using an in vitro method, and this information was used to build synthetic promoters. The promoters and repressors were screened for cross-reactions. Of these, 16 were identified that both strongly repress their cognate promoter (5- to 207-fold) and exhibit minimal interactions with other promoters. Each repressor-promoter pair was converted to a NOT gate and characterized. Used as a set of 16 NOT/NOR gates, there are >10[superscript 54] circuits that could be built by changing the pattern of input and output promoters. This represents a large set of compatible gates that can be used to construct user-defined circuits., United States. Air Force Office of Scientific Research (Award FA9550-11-C-0028), American Society for Engineering Education. National Defense Science and Engineering Graduate Fellowship (32 CFR 168a), United States. Defense Advanced Research Projects Agency. Chronical of Lineage Indicative of Origins (N66001-12-C-4016), United States. Office of Naval Research (N00014-13-1-0074), National Institutes of Health (U.S.) (GM095765), National Science Foundation (U.S.). Synthetic Biology Engineering Research Center (SA5284-11210)

Cells react to their environment through gene regulatory networks. Network integrity requires minimization of undesired crosstalk between their biomolecules. Similar constraints also limit the use of regulators when building synthetic circuits for engineering applications. Here, we mapped the promoter specificities of extracytoplasmic function (ECF) σs as well as the specificity of their interaction with anti‐σs. DNA synthesis was used to build 86 ECF σs (two from every subgroup), their promoters, and 62 anti‐σs identified from the genomes of diverse bacteria. A subset of 20 σs and promoters were found to be highly orthogonal to each other. This set can be increased by combining the −35 and −10 binding domains from different subgroups to build chimeras that target sequences unrepresented in any subgroup. The orthogonal σs, anti‐σs, and promoters were used to build synthetic genetic switches in Escherichia coli. This represents a genome‐scale resource of the properties of ECF σs and a resource for synthetic biology, where this set of well‐characterized regulatory parts will enable the construction of sophisticated gene expression programs., Life Technologies, Inc., United States. Defense Advanced Research Projects Agency (Chronicle of Lineage Indicative of Origins N66001-12-C-4018), United States. Office of Naval Research (N00014-10-1-0245), National Institutes of Health (U.S.) (NIH AI067699), National Science Foundation (U.S.). Synthetic Biology Engineering Research Center (SA5284-11210), American Society for Engineering Education. National Defense Science and Engineering Graduate Fellowship, Hertz Foundation (Fellowship)

NASA Science Mission Directorate's In-Space Propulsion Technology Program is sponsoring the development of a 3.8 kW-class engineering development unit Hall thruster for implementation in NASA science and exploration missions. NASA Glenn Research Center and Aerojet are developing a high fidelity high voltage Hall accelerator (HiVHAc) thruster that can achieve specific impulse magnitudes greater than 2,700 seconds and xenon throughput capability in excess of 300 kilograms. Performance, plume mappings, thermal characterization, and vibration tests of the HiVHAc engineering development unit thruster have been performed. In addition, the HiVHAc project is also pursuing the development of a power processing unit (PPU) and xenon feed system for integration with the HiVHAc engineering development unit thruster. Colorado Power Electronics and NASA Glenn Research Center have tested a brassboard PPU for more than 1,500 hours in a vacuum environment, and a new brassboard and engineering model PPU units are under development. VACCO Industries developed a xenon flow control module which has undergone qualification testing and will be integrated with the HiVHAc thruster extended duration tests. Finally, recent mission studies have shown that the HiVHAc propulsion system has sufficient performance for four Discovery- and two New Frontiers-class NASA design reference missions.

The In-Space Propulsion Technology (ISPT) program has been developing in-space propulsion technologies that will enable or enhance NASA robotic science missions. The ISPT program is currently developing technology in four areas that include Propulsion System Technologies (Electric and Chemical), Entry Vehicle Technologies (Aerocapture and Earth entry vehicles), Spacecraft Bus and Sample Return Propulsion Technologies (components and ascent vehicles), and Systems/Mission Analysis. Three technologies are ready for flight infusion: 1) the high-temperature Advanced Material Bipropellant Rocket (AMBR) engine providing higher performance; 2) NASA s Evolutionary Xenon Thruster (NEXT) ion propulsion system, a 0.6-7 kW throttle-able gridded ion system; and 3) Aerocapture technology development with investments in a family of thermal protection system (TPS) materials and structures; guidance, navigation, and control (GNC and aerothermal effect models. Two component technologies that will be ready for flight infusion in the near future will be Advanced Xenon Flow Control System, and ultra-lightweight propellant tank technologies. Future focuses for ISPT are sample return missions and other spacecraft bus technologies like: 1) Mars Ascent Vehicles (MAV); 2) multi-mission technologies for Earth Entry Vehicles (MMEEV) for sample return missions; and 3) electric propulsion for sample return and low cost missions. These technologies are more vehicle-focused, and present a different set of technology infusion challenges. While the Systems/Mission Analysis area is focused on developing tools and assessing the application of propulsion technologies to a wide variety of mission concepts. These in-space propulsion technologies are applicable, and potentially enabling for future NASA Discovery, New Frontiers, and sample return missions currently under consideration, as well as having broad applicability to potential Flagship missions. This paper provides a brief overview of the ISPT program, describing the development status and technology infusion readiness of in-space propulsion technologies in the areas of electric propulsion, aerocapture, Earth entry vehicles, propulsion components, Mars ascent vehicle, and mission/systems analysis.