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10 results on '"Tocheny, Claire E."'

6. Rapid GPR183-mediated recruitment of eosinophils to the lung after Mycobacterium tuberculosis infection

Author

Bohrer, Andrea C., primary, Castro, Ehydel, additional, Tocheny, Claire E., additional, Assmann, Maike, additional, Schwarz, Benjamin, additional, Bohrnsen, Eric, additional, Makiya, Michelle A., additional, Legrand, Fanny, additional, Hilligan, Kerry L., additional, Baker, Paul J., additional, Torres-Juarez, Flor, additional, Hu, Zhidong, additional, Ma, Hui, additional, Wang, Lin, additional, Niu, Liangfei, additional, Wen, Zilu, additional, Lee, Sang H., additional, Kamenyeva, Olena, additional, Kauffman, Keith D., additional, Donato, Michele, additional, Sher, Alan, additional, Barber, Daniel L., additional, Via, Laura E., additional, Scriba, Thomas J., additional, Khatri, Purvesh, additional, Song, Yanzheng, additional, Wong, Ka-Wing, additional, Bosio, Catharine M., additional, Klion, Amy D., additional, and Mayer-Barber, Katrin D., additional

Published
2022
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9. Eosinophils are part of the granulocyte response in tuberculosis and promote host resistance in mice

Author

Bohrer, Andrea C., Castro, Ehydel, Hu, Zhidong, Queiroz, Artur T.L., Tocheny, Claire E., Assmann, Maike, Sakai, Shunsuke, Nelson, Christine, Baker, Paul J., Ma, Hui, Wang, Lin, Zilu, Wen, du Bruyn, Elsa, Riou, Catherine, Kauffman, Keith D., Moore, Ian N., Del Nonno, Franca, Petrone, Linda, Goletti, Delia, Martineau, Adrian R., Lowe, David M., Cronan, Mark R., Wilkinson, Robert J., Barry, Clifton E., Via, Laura E., Barber, Daniel L., Klion, Amy D., Andrade, Bruno B., Song, Yanzheng, Wong, Ka-Wing, and Mayer-Barber, Katrin D.

Abstract

Host resistance to Mycobacterium tuberculosis (Mtb) infection requires the activities of multiple leukocyte subsets, yet the roles of the different innate effector cells during tuberculosis are incompletely understood. Here we uncover an unexpected association between eosinophils and Mtb infection. In humans, eosinophils are decreased in the blood but enriched in resected human tuberculosis lung lesions and autopsy granulomas. An influx of eosinophils is also evident in infected zebrafish, mice, and nonhuman primate granulomas, where they are functionally activated and degranulate. Importantly, using complementary genetic models of eosinophil deficiency, we demonstrate that in mice, eosinophils are required for optimal pulmonary bacterial control and host survival after Mtb infection. Collectively, our findings uncover an unexpected recruitment of eosinophils to the infected lung tissue and a protective role for these cells in the control of Mtb infection in mice.

Published
2021
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10. Subcellular patterns of SPE-6 localization reveal unexpected complexities in Caenorhabditis elegans sperm activation and sperm function.

Author

Peterson JJ, Tocheny CE, Prajapati G, LaMunyon CW, and Shakes DC

Subjects
Animals, Chromosomes, Female, Male, Mutation, Spermatogenesis, Spermatozoa, Caenorhabditis elegans genetics, Caenorhabditis elegans Proteins genetics
Abstract

To acquire and maintain directed cell motility, Caenorhabditis elegans sperm must undergo extensive, regulated cellular remodeling, in the absence of new transcription or translation. To regulate sperm function, nematode sperm employ large numbers of protein kinases and phosphatases, including SPE-6, a member of C. elegans' highly expanded casein kinase 1 superfamily. SPE-6 functions during multiple steps of spermatogenesis, including functioning as a "brake" to prevent premature sperm activation in the absence of normal extracellular signals. Here, we describe the subcellular localization patterns of SPE-6 during wild-type C. elegans sperm development and in various sperm activation mutants. While other members of the sperm activation pathway associate with the plasma membrane or localize to the sperm's membranous organelles, SPE-6 surrounds the chromatin mass of unactivated sperm. During sperm activation by either of two semiautonomous signaling pathways, SPE-6 redistributes to the front, central region of the sperm's pseudopod. When disrupted by reduction-of-function alleles, SPE-6 protein is either diminished in a temperature-sensitive manner (hc187) or is mislocalized in a stage-specific manner (hc163). During the multistep process of sperm activation, SPE-6 is released from its perinuclear location after the spike stage in a process that does not require the fusion of membranous organelles with the plasma membrane. After activation, spermatozoa exhibit variable proportions of perinuclear and pseudopod-localized SPE-6, depending on their location within the female reproductive tract. These findings provide new insights regarding SPE-6's role in sperm activation and suggest that extracellular signals during sperm migration may further modulate SPE-6 localization and function., (© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.)

Published
2021
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