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Your search keyword '"Tobery, Timothy W."' showing total 17 results
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17 results on '"Tobery, Timothy W."'

1. Detection of HIV vaccine-induced cell-mediated immunity in HIV-seronegative clinical trial participants using an optimized and validated enzyme-linked immunospot assay

Author

Dubey, Sheri, Clair, James, Tong-Ming Fu, Liming Guan, Long, Romnie, Mogg, Robin, Anderson, Kiersten, Collins, Kelly B., Gaunt, Christine, Fernandez, V. Rose, Lan Zhu, Kierstead, Lisa, Thaler, Scott, Gupta, Swati B., Straus, Walter, Mehrotra, Devan, Tobery, Timothy W., Casimiro, Danilo R., and Shiver, John W.

Subjects
AIDS vaccines -- Research, AIDS vaccines -- Patient outcomes, Immunoenzyme technique -- Usage, Immune response -- Measurement, Health
Abstract

The interferon-[gamma] (IFN[gamma]) enzyme-linked immunospot (ELISPOT) assay is used to detect a broad range of HIV-specific ELISPOT responses to peptide pools of overlapping 22mers, 15mers, or 9mers in study volunteers receiving DNA- or adenovirus vector-based HIV vaccines and in HIV-seropositive donors. The results have shown that the validated ELISPOT assay using 15mer peptide pools and the positivity criteria is a sensitive and specific assay for the detection of HIV vaccine-induced cell-mediated immunity.

Published
2007

6. Transporter-independent processing of HIV-1 envelope protein for recognition by CD8+ T cells

Author

Hammond, Scott A., Bollinger, Robert C., Tobery, Timothy W., and Siliciano, Robert F.

Subjects
Peptides -- Transportation, Cell research -- Observations, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

An analysis shows that the transportation of cytosolic peptides into the endoplasmic reticulum for a connection with class I molecules is necessary and may involve a heterodimer of the MHC-encoded proteins, Tap-1 and Tap-2 12-17. The analysis is based on the theory that CD8 cytolic T lymphocytes (CTT) determine virally infected cells by identifying processed viral antigen associating with class I major histocompatibility complex (MHC) molecules on infected cells. Results reveal that the HIV-1 envelope (env) protein in infected cells is processed by a new Tap-1/Tap-2 independent pathway that is localized to the endoplasmic reticulum.

Published
1993

8. Efficacy of Multivalent Adenovirus-Based Vaccine against Simian Immunodeficiency Virus Challenge

Author

Casimiro, Danilo R., primary, Cox, Kara, additional, Tang, Aimin, additional, Sykes, Kara J., additional, Feng, Meizhen, additional, Wang, Fubao, additional, Bett, Andrew, additional, Schleif, William A., additional, Liang, Xiaoping, additional, Flynn, Jessica, additional, Tobery, Timothy W., additional, Wilson, Keith, additional, Finnefrock, Adam, additional, Huang, Lingyi, additional, Vitelli, Salvatore, additional, Lin, Jing, additional, Patel, Deepa, additional, Davies, Mary-Ellen, additional, Heidecker, Gwendolyn J., additional, Freed, Daniel C., additional, Dubey, Sheri, additional, O'Connor, David H., additional, Watkins, David I., additional, Zhang, Zhi-Qiang, additional, and Shiver, John W., additional

Published
2010
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9. Enhanced Rates and Magnitude of Immune Responses Detected against an HIV Vaccine: Effect of Using an Optimized Process for Isolating PBMC

Author

Kierstead, Lisa S., primary, Dubey, Sheri, additional, Meyer, Barbara, additional, Tobery, Timothy W., additional, Mogg, Robin, additional, Fernandez, V. Rose, additional, Long, Romnie, additional, Guan, Liming, additional, Gaunt, Christine, additional, Collins, Kelly, additional, Sykes, Kara J., additional, Mehrotra, Devan V., additional, Chirmule, Narendra, additional, Shiver, John W., additional, and Casimiro, Danilo R., additional

Published
2007
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10. A Comparison of Standard Immunogenicity Assays for Monitoring HIV Type 1 gag-Specific T Cell Responses in Ad5 HIV Type 1 gag Vaccinated Human Subjects

Author

Tobery, Timothy W., primary, Dubey, Sheri A., additional, Anderson, Kiersten, additional, Freed, Daniel C., additional, Cox, Kara S., additional, Lin, Jing, additional, Prokop, Michael T., additional, Sykes, Kara J., additional, Mogg, Robin, additional, Mehrotra, Devan V., additional, Fu, Tong-Ming, additional, Casimiro, Danilo R., additional, and Shiver, John W., additional

Published
2006
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11. Attenuation of Simian Immunodeficiency Virus SIVmac239 Infection by Prophylactic Immunization with DNA and Recombinant Adenoviral Vaccine Vectors Expressing Gag

Author

Casimiro, Danilo R., primary, Wang, Fubao, additional, Schleif, William A., additional, Liang, Xiaoping, additional, Zhang, Zhi-Qiang, additional, Tobery, Timothy W., additional, Davies, Mary-Ellen, additional, McDermott, Adrian B., additional, O'Connor, David H., additional, Fridman, Arthur, additional, Bagchi, Ansu, additional, Tussey, Lynda G., additional, Bett, Andrew J., additional, Finnefrock, Adam C., additional, Fu, Tong-ming, additional, Tang, Aimin, additional, Wilson, Keith A., additional, Chen, Minchun, additional, Perry, Helen C., additional, Heidecker, Gwendolyn J., additional, Freed, Daniel C., additional, Carella, Anthony, additional, Punt, Kara S., additional, Sykes, Kara J., additional, Huang, Lingyi, additional, Ausensi, Virginia I., additional, Bachinsky, Margaret, additional, Sadasivan-Nair, Usha, additional, Watkins, David I., additional, Emini, Emilio A., additional, and Shiver, John W., additional

Published
2005
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12. Real-Time Monitoring of Bacterial Infection In Vivo: Development of Bioluminescent Staphylococcal Foreign-Body and Deep-Thigh-Wound Mouse Infection Models

Author

Kuklin, Nelly A., primary, Pancari, Gregory D., additional, Tobery, Timothy W., additional, Cope, Leslie, additional, Jackson, Jesse, additional, Gill, Charles, additional, Overbye, Karen, additional, Francis, Kevin P., additional, Yu, Jun, additional, Montgomery, Donna, additional, Anderson, Annaliesa S., additional, McClements, William, additional, and Jansen, Kathrin U., additional

Published
2003
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13. Sustained Peptide-Specific Gamma Interferon T-Cell Response in Rhesus Macaques Immunized with Human Immunodeficiency Virus gag DNA Vaccines

Author

Caulfield, Michael J., primary, Wang, Su, additional, Smith, Jeffrey G., additional, Tobery, Timothy W., additional, Liu, Xu, additional, Davies, Mary-Ellen, additional, Casimiro, Danilo R., additional, Fu, Tong-Ming, additional, Simon, Adam, additional, Evans, Robert K., additional, Emini, Emilio A., additional, and Shiver, John, additional

Published
2002
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14. Identification of T-Cell Epitopes Using ELISpot and Peptide Pool Arrays.

Author

Walker, John M., Decler, Jochen, Reischl, Udo, Tobery, Timothy W., and Caulfield, Michael J.

Abstract

Here we describe a method for T-cell epitope identification using a modified ELISpot assay that is both simple and efficient. By using a carefully constructed array of pools of overlapping peptides spanning the entire antigen sequence to stimulate T-cell responses, we are able to detect antigen-specific cytokine responses by both CD8+ and CD4+ T cells and identify the specific peptides to which the cells are responding. Additionally, by performing magnetic bead depletion of either CD8+ or CD4+ cells prior to the assay, we are able to determine the phenotype of the responding cells to each of the peptide epitopes identified. Use of this method will allow the identification of both CD4+ and CD8+ T-cell epitopes without the need for MHC allele-matched reagents and without the need for highly specialized instrumentation. By using an array of peptide pools, this method also dramatically reduces the number of immune cells required to test the entire antigen sequence, often a limiting factor in vaccine testing and other studies. [ABSTRACT FROM AUTHOR]

Published
2004
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16. Sustained Peptide-Specific Gamma Interferon T-Cell Response in Rhesus Macaques Immunized with Human Immunodeficiency Virus gagDNA Vaccines

Author

Caulfield, Michael J., Wang, Su, Smith, Jeffrey G., Tobery, Timothy W., Liu, Xu, Davies, Mary-Ellen, Casimiro, Danilo R., Fu, Tong-Ming, Simon, Adam, Evans, Robert K., Emini, Emilio A., and Shiver, John

Abstract

ABSTRACTWe examined the influence of dose and method of antigen delivery on the dynamics and durability of T-cell responses to candidate human immunodeficiency virus (HIV) vaccines. Codon-optimized sequences from the HIV gaggene were inserted into alternative DNA vaccine vectors to express the coding sequence with or without the tissue plasminogen activator leader sequence. We delivered the vaccines by intramuscular injection as plasmid DNA without adjuvant or as plasmid DNA formulated with a novel block copolymer adjuvant (CRL8623) and then monitored the ensuing T-cell responses by using a gamma interferon enzyme-linked immunospot assay. We demonstrated persistence of the cell-mediated immune (CMI) response in rhesus macaques for at least 18 months following a four-dose vaccination regimen. The plasmid vaccine, with or without CRL8623, was immunogenic in macaques; however, the form coadministered with adjuvant exhibited improved T-cell responses, with a bias toward more antigen-specific CD8+T cells. Finally, we examined the fine specificity of the T-cell response to the gagvaccines by testing the response of 23 vaccinated macaques to individual Gag 20-mer peptides. Collectively, the monkeys responded to 25 epitopes, and, on average, each monkey recognized a minimum of 2.7 epitopes. The results indicate that a broad and durable CMI response to HIV DNA vaccines can be induced in a relevant nonhuman primate model.

Published
2002
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17. Identification of T-cell epitopes using ELISpot and peptide pool arrays.

Author

Tobery TW and Caulfield MJ

Subjects
Amino Acid Sequence, Animals, Antigens, Viral chemistry, Antigens, Viral genetics, Epitopes genetics, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Oncogene Proteins, Viral chemistry, Oncogene Proteins, Viral genetics, Oncogene Proteins, Viral immunology, Papillomaviridae genetics, Papillomaviridae immunology, Peptides genetics, Peptides immunology, Peptides isolation & purification, Protein Array Analysis, Enzyme-Linked Immunosorbent Assay methods, Epitopes isolation & purification, T-Lymphocytes immunology
Abstract

Here we describe a method for T-cell epitope identification using a modified ELISpot assay that is both simple and efficient. By using a carefully constructed array of pools of overlapping peptides spanning the entire antigen sequence to stimulate T-cell responses, we are able to detect antigen-specific cytokine responses by both CD8+ and CD4+ T cells and identify the specific peptides to which the cells are responding. Additionally, by performing magnetic bead depletion of either CD8+ or CD4+ cells prior to the assay, we are able to determine the phenotype of the responding cells to each of the peptide epitopes identified. Use of this method will allow the identification of both CD4+ and CD8+ T-cell epitopes without the need for MHC allele-matched reagents and without the need for highly specialized instrumentation. By using an array of peptide pools, this method also dramatically reduces the number of immune cells required to test the entire antigen sequence, often a limiting factor in vaccine testing and other studies.

Published
2004
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