1. Development and potential use of an Edwardsiella ictaluri wzz mutant as a live attenuated vaccine against enteric septicemia in Pangasius hypophthalmus (Tra catfish).
- Author
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Triet TH, Tinh BTT, Hau LV, Huong TV, and Binh NQ
- Subjects
- Animals, Bacterial Vaccines genetics, Catfishes microbiology, Edwardsiella ictaluri genetics, Enterobacteriaceae Infections immunology, Enterobacteriaceae Infections prevention & control, Fish Diseases immunology, Fish Diseases microbiology, Gene Deletion, Mutation, Vaccines, Attenuated genetics, Vaccines, Attenuated immunology, Bacterial Vaccines immunology, Catfishes immunology, Edwardsiella ictaluri immunology, Enterobacteriaceae Infections veterinary, Fish Diseases prevention & control
- Abstract
Edwardsiella ictaluri is a causative agent of enteric septicemia of catfish (ESC), a seriously lethal disease in Vietnamese catfish (Pangasius hypophthalmus). A safe and effective vaccine against ESC is currently an urgent demand due to antibiotic overuse in pangasius farms has led to an alarming antimicrobial resistance. In this study, two E. ictaluri wzzE mutants (WzM-L3, deficient in a 1038bp-entire wzzE gene and WzM-S3, a 245bp-partial deletion of wzzE) were developed and their protection efficiacy was evaluated in hatched pangasius against ESC by immersion vaccination. As comparing to the high virulent wild-type strain who caused 73.33% of death on pangasius fingerlings immersed at 7.1 × 10
6 CFU ml-1 , both mutants showed extremely low mortality rates at 3.33% (WzM-S3) and 0% (WzM-L3) on pangasius fingerlings immersed at high concentration of 1.5 × 107 CFU mL-1 and 9.7 × 106 CFU ml-1 , respectively. Interestingly, both WzM-S3 and WzM-L3 had a remarkably high protection against ESC, as RPS % were found at 89.29% and 90%, respectively. The mutant WzM-L3 is a potential live attenuated vaccine against ESC in Vietnamese catfish farms with good protection and simple practice., (Copyright © 2019. Published by Elsevier Ltd.)- Published
- 2019
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