7 results on '"Tingxia Zhang"'
Search Results
2. Presence of Residual Cardiac Thrombus Predicts Poor Outcome in Cardioembolic Stroke After Reperfusion Therapy
- Author
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Tingxia Zhang, Huan Zhou, Jiansheng Yang, Ying Zhou, Yi Chen, Yaode He, Rui Xue, Zhicai Chen, Min Lou, and Shenqiang Yan
- Subjects
cardiac imaging ,cardioembolic stroke ,functional outcome ,thrombolysis ,thrombus ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background In patients with acute cardiogenic cerebral embolism, a residual thrombus may still be present in the cardiac cavity even after reperfusion therapy. We aimed to investigate the occurrence of a residual cardiac thrombus in cardioembolic stroke after reperfusion therapy and analyze its impact on clinical outcome. Methods and Results We enrolled patients with cardioembolic stroke from our prospectively collected database who underwent 2‐phase cardiac computed tomography within 7 days after reperfusion therapy. Residual cardiac thrombus was defined as a filling defect on both early‐ and late‐phase images, whereas circulatory stasis was defined as a filling defect only on the early‐phase images in the left atrial appendage. The primary outcome was a poor clinical outcome (modified Rankin Scale score, 3–6) at 90 days. The secondary outcome was a composite end point event (cardiovascular death, nonfatal myocardial infarction, nonfatal stroke) at 90 days. A total of 303 patients were included, of whom 94 (31.0%) had a residual cardiac thrombus. Binary logistic regression analysis showed that the presence of a residual cardiac thrombus was associated with a poor clinical outcome (odds ratio, 1.951 [95% CI, 1.027–3.707]; P=0.041) but not circulatory stasis in the left atrial appendage (odds ratio, 1.096 [95% CI, 0.542–2.217]; P=0.798). Furthermore, there was no correlation between a residual cardiac thrombus and the composite end point event (30.0% versus 31.1%; P=1.000). Conclusions Residual cardiac thrombus occurs in approximately one‐third of patients with cardioembolic stroke after reperfusion therapy and is often indicative of a poor clinical outcome.
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- 2024
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3. ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer
- Author
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Mei Li, Yanan Zheng, Xujun Li, Xiaohan Shen, Tingxia Zhang, Bowen Weng, Haijiao Mao, and Jiyuan Zhao
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ATBF1 ,Mislocalization ,Histological grade ,Cell differentiation ,WNT5A ,Breast cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Histological grade has been demonstrated to be an important factor of breast cancer outcome and is associated with cell differentiation and is currently being evaluated via H&E-stained sections. Molecular biomarkers are essential to improve the accuracy of histological grading. ATBF1, a large transcription factor, has been considered a tumor suppressor gene with frequent mutations or deletions in multiple cancers. In breast cancer, ATBF1 was reported to function in cell differentiation and mammary development. However, its role in the clinic has rarely been reported. Methods Breast cancer tissues (BCTs) and adjacent noncancerous tissues (ANCTs) were collected to analyze the expression of ATBF1 at the mRNA and protein levels. Three anti-ATBF1 antibodies recognizing independent peptides of ATBF1 (N-terminal end, middle region and C-terminal end) were applied for IHC staining. Small interfering RNA (siRNA) was used to silence ATBF1 expression and to investigate the roles of ATBF1 in MCF7 cells. Microarrays were introduced to analyze the differentially expressed genes, enriched GO terms and KEGG terms regulated by ATBF1 and its potential downstream genes, which were further confirmed in vitro and in clinical samples. Results The expression of ATBF1 was reduced in BCTs at both the mRNA and protein levels compared with that in ANCTs. ATBF1 protein was predominantly localized in the nucleus of ANCTs but in the cytoplasm of BCTs. Both the mRNA and protein levels of ATBF1 were significantly correlated with histological grade. Consistently, knockdown of ATBF1 increased stemness marker expression and reduced differentiation markers in vitro. Further analysis identified WNT5A as an essential downstream gene of ATBF1 in breast cancer cells. Treatment of WNT5A disrupted cell proliferation induced by ATBF1 silencing. In BCTs, a significant correlation was observed between the expression of WNT5A and ATBF1. Conclusion The results indicated that ATBF1 expression might be a useful diagnostic marker associated with histological grade and breast cancer malignancy. WNT5A and its signaling pathway are novel mechanisms by which ATBF1 contributes to breast cancer tumorigenesis.
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- 2022
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4. Comparing Outcomes of Thrombectomy Versus Intravenous Thrombolysis Based on Middle Cerebral Artery M2 Occlusion Features.
- Author
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Huan Zhou, Wansi Zhong, Tingxia Zhang, Chenghua Xu, Genlong Zhong, Guomin Xie, Bing Zhang, Hongfang Chen, En Wang, Dongjuan Xu, Chaochan Cheng, Jiansheng Yang, Min Lou, and Shenqiang Yan
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- 2024
- Full Text
- View/download PDF
5. [Research progress of decellularized extracellular matrix hydrogel in regenerative medicine]
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Jingyu, Jiang, Chi, Zhang, Tingxia, Zhang, and Jiyuan, Zhao
- Subjects
Tissue Engineering ,Tissue Scaffolds ,综 述 ,Humans ,Hydrogels ,Regenerative Medicine ,Extracellular Matrix - Abstract
Decellularized extracellular matrix (dECM) has been widely used as a scaffold for regenerative medicine due to its high biomimetic and excellent biocompatibility. As a functional polymer material with high water content and controlled fluidity, hydrogel is very promising for some minimally invasive surgery in clinical practice. In recent years, with the rapid development of hydrogel theory and technology, dECM hydrogel has gradually become a research hotspot in the field of regenerative medicine. In this paper, the related researches in recent years are reviewed regarding the preparation of dECM hydrogel and its preclinical application. The future clinical use is also prospected.脱细胞基质(dECM)由于其高仿生性和优异的生物相容性,已被广泛用作再生医学的支架材料。水凝胶(hydrogel)作为一种高含水量、可控流动性的功能高分子材料,非常适合用于临床中的部分微创手术。近年来,随着水凝胶理论和技术的快速发展,dECM 水凝胶逐渐成为再生医学领域的研究热点。本文从 dECM 水凝胶的制备及其临床前应用两个方面对近年来的相关研究进行综述,并展望其未来的临床前景。.
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- 2020
6. Small intestinal submucosa/polymethyl methacrylate composite bone cement for vertebral repair
- Author
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Chi Zhang, Jinjin Zhu, Tingxia Zhang, Jiyuan Zhao, Mei Li, and Guoqiang Jiang
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Materials science ,medicine.medical_treatment ,0206 medical engineering ,Composite number ,macromolecular substances ,02 engineering and technology ,Osseointegration ,Percutaneous vertebroplasty ,lcsh:TA401-492 ,medicine ,General Materials Science ,Bone regeneration ,Cement ,Mechanical Engineering ,Vertebral compression fracture ,technology, industry, and agriculture ,equipment and supplies ,021001 nanoscience & nanotechnology ,Bone cement ,medicine.disease ,020601 biomedical engineering ,body regions ,Compressive strength ,Mechanics of Materials ,lcsh:Materials of engineering and construction. Mechanics of materials ,0210 nano-technology ,Biomedical engineering - Abstract
Percutaneous vertebroplasty (PVP) and kyphoplasty (PKP) are widely used to treat patients who suffer painful vertebral compression fractures. Both procedures involve the injection of filling materials into the fractured vertebra. In the clinic, the most commonly used bone cement is polymethyl methacrylate (PMMA). However, it has some inherent disadvantages, including poor osteoinductivity and excessive stiffness when used for vertebroplasty. The objective of this study was to develop novel small intestinal submucosa/polymethyl methacrylate (SIS/PMMA) composite bone cement for vertebral repair. The SIS/PMMA composite cement had a porous three-dimensional structure with lower compressive strength and compressive modulus than commercially available PMMA. Both MC3T3-E1 cells and bone marrow mesenchymal stem cells (BMSCs) adhered better to the SIS/PMMA composites than to PMMA. In addition, cell proliferation and osteogenic differentiation of MC3T3-E1 cells were enhanced when cultured on the SIS/PMMA composites. In a vertebral defect model, the SIS/PMMA composites greatly enhanced osteointegration, tissue union, and bone regeneration. In conclusion, this work suggests that the SIS/PMMA composite can be used to treat vertebral compression fracture. Keywords: Percutaneous vertebroplasty, Polymethyl methacrylate, Small intestinal submucosa, Biocompatibility, Compressive modulus, Bone regeneration
- Published
- 2018
7. ECM coating modification generated by optimized decellularization process improves functional behavior of BMSCs
- Author
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Tingxia Zhang, Jingyu Jiang, Mei Li, Yuxing Mao, Jiyuan Zhao, and Anqi Zhang
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Homeobox protein NANOG ,Materials science ,Bioengineering ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Regenerative medicine ,Cell Line ,Biomaterials ,Extracellular matrix ,Mice ,Calcification, Physiologic ,stomatognathic system ,Tissue engineering ,Animals ,Rats, Wistar ,Cells, Cultured ,Cell Proliferation ,Glycosaminoglycans ,Decellularization ,biology ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,DNA ,021001 nanoscience & nanotechnology ,Extracellular Matrix ,0104 chemical sciences ,Cell biology ,Fibronectin ,Gene Expression Regulation ,Mechanics of Materials ,biology.protein ,Collagen ,Stem cell ,0210 nano-technology ,Biomarkers - Abstract
Bone mesenchymal stem cells (BMSCs) have been widely applied in tissue engineering and regenerative medicine. However, small number of BMSCs and loss of stem cell characteristics after expansion in vitro limited clinical use of BMSCs. In the present study, osteoblasts were cultured to lay down extracellular matrix (ECM) and then the cells were removed (decellularization) to generate ECM coating substrates. The decellularization process was optimized to maximally remove cells and cellular components, along with integrated ECM retained which was demonstrated to be beneficial for BMSCs expansion in vitro. After decellularization, only less than 2% of residual DNA and cellular proteins were detected in TFFF-ECM (decellularized by triton X-100 (T) and three freeze/thaw cycles (FFF)), which was much less than that in TN-ECM generated by traditional decellularization method (triton X-100 (T) and NH4OH (N)). Meanwhile, ECM components and structure were preserved best after decellularization by TFFF method. More ECM proteins were detected, and structure proteins (fibronectin and collagen) exhibited as classic network fibers in TFFF-ECM. Functionally, all kinds of decellularized ECM (dECM) were demonstrated to promote BMSCs proliferation and osteogenic differentiation capacity, thus maintain the stemness of BMSCs. Importantly, cells cultured on TFFF-ECM grew faster than the cells on other kinds of dECM at early stage and TFFF-ECM was beneficial to preserve stemness of BMSCs with high expression of OCT4 and NANOG when cultured in vitro. Proteomic analysis showed the proteins in ECM functioned in multiple biological activities and signaling pathways, which contributed to stemness maintenance of BMSC. Thus, the mild decellularization process optimized in this study enhanced the effectiveness of dECM for BMSCs culture in vitro and maybe further applied to BMSCs based tissue repair.
- Published
- 2019
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