33 results on '"Ting-Ting Yi"'
Search Results
2. Glycyrrhizic acid protects against temporal lobe epilepsy in young rats by regulating neuronal ferroptosis through the miR‐194‐5p/PTGS2 axis
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Ting‐Ting Yi, Li‐Mei Zhang, and Xiang‐Nan Huang
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ferroptosis ,glycyrrhizic acid ,miR‐194‐5p ,neurons ,temporal lobe epilepsy ,Medicine (General) ,R5-920 - Abstract
Abstract Temporal lobe epilepsy (TLE) leads to extensive degradation of the quality of life of patients. Glycyrrhizic acid (GA) has been reported to exert neuroprotective effects on status epilepticus. Herein, the current study set out to explore the functional mechanism of GA in TLE young rats. Firstly, TLE young rat models were established using the lithium chloride and pilocarpine regimen and then subjected to treatment with different doses of GA, miR‐194‐5p‐antagomir, or/and sh‐prostaglandin‐endoperoxide synthase 2 (PTGS2) to observe changes in iron content, glutathione and malondialdehyde levels, and GPX4 (glutathione peroxidase 4) and PTGS2 protein levels in the hippocampus. Neuronal injury and apoptosis were assessed through HE, Nissl, and TUNEL staining. Additionally, the expression patterns of miR‐194‐5p were detected. The binding site of miR‐194‐5p and PTGS2 was verified with a dual‐luciferase assay. Briefly, different doses of GA (20, 40, and 60 mg/kg) reduced the epileptic score, frequency, and duration in TLE young rats, along with reductions in iron content, lipid peroxidation, neuronal injury, and apoptosis in the hippocampus. Silencing of miR‐194‐5p partly annulled the action of GA on inhibiting ferroptosis and attenuating neuronal injury in TLE young rats. Additionally, PTGS2 was validated as a target of miR‐194‐5p. GA inhibited ferroptosis and ameliorated neuronal injury in TLE young rats via the miR‐194‐5p/PTGS2 axis. Overall, our findings indicated that GA exerts protective effects on TLE young rats against neuronal injury by inhibiting ferroptosis through the miR‐194‐5p/PTGS2 axis.
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- 2023
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3. A survey of current practices, attitudes and demands of anaesthesiologists regarding the depth of anaesthesia monitoring in China
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Jian Zhan, Ting-Ting Yi, Zhuo-Xi Wu, Zong-Hong Long, Xiao-Hang Bao, Xu-Dong Xiao, Zhi-Yong Du, Ming-Jun Wang, and Hong Li
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Depth of anaesthesia ,Anaesthesiologists ,Awareness ,Analgesia ,Artificial intelligence ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Background In this study, we aimed to analyse survey data to explore two different hypotheses; and for this purpose, we distributed an online survey to Chinese anaesthesiologists. The hypothetical questions in this survey include: (1) Chinese anaesthesiologists mainly use the depth of anaesthesia (DoA) monitors to prevent intraoperative awareness and (2) the accuracy of these monitors is the most crucial performance factor during the clinical daily practice of Chinese anaesthesiologists. Methods We collected and statistically analysed the response of a total of 12,750 anesthesiologists who were invited to participate in an anonymous online survey. The Chinese Society of Anaesthesiologists (CSA) trial group provided the email address of each anaesthesiologist, and the selection of respondents was random from the computerized system. Results The overall response rate was 32.0% (4037 respondents). Only 9.1% (95% confidence interval, 8.2-10.0%) of the respondents routinely used DoA monitors. Academic respondents (91.5, 90.3-92.7%) most frequently used DoA monitoring to prevent awareness, whereas nonacademic respondents (88.8, 87.4-90.2%) most frequently used DoA monitoring to guide the delivery of anaesthetic agents. In total, the number of respondents who did not use a DoA monitor and whose patients experienced awareness (61.7, 57.8-65.6%) was significantly greater than those who used one or several DoA monitors (51.5, 49.8-53.2%). Overall, the crucial performance factor during DoA monitoring was considered by 61.9% (60.4-63.4%) of the respondents to be accuracy. However, most respondents (95.7, 95.1-96.3%) demanded improvements in the accuracy of the monitors for DoA monitoring. In addition, broad application in patients of all ages (86.3, 85.2-87.4%), analgesia monitoring (80.4, 79.2-81.6%), and all types of anaesthetic agents (75.6, 74.3-76.9%) was reported. In total, 65.0% (63.6-66.5%) of the respondents believed that DoA monitors should be combined with EEG and vital sign monitoring, and 53.7% (52.1-55.2%) believed that advanced DoA monitors should include artificial intelligence. Conclusions Academic anaesthesiologists primarily use DoA monitoring to prevent awareness, whereas nonacademic anaesthesiologists use DoA monitoring to guide the delivery of anaesthetics. Anaesthesiologists demand high-accuracy DoA monitors incorporating EEG signals, multiple vital signs, and antinociceptive indicators. DoA monitors with artificial intelligence may represent a new direction for future research on DoA monitoring.
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- 2021
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4. Glycyrrhizic acid protects against temporal lobe epilepsy in young rats by regulating neuronal ferroptosis through the <scp>miR</scp> ‐194‐5p/ <scp>PTGS2</scp> axis
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Ting‐Ting Yi, Li‐Mei Zhang, and Xiang‐Nan Huang
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General Medicine - Published
- 2023
5. Current practices, attitudes and demands of Chinese anesthesiologists regarding depth of anesthesia monitoring
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Jian Zhan, Ting-Ting Yi, Zhuo-Xi Wu, Zong-Hong Long, Xiao-Hang Bao, Xu-Dong Xiao, Zhi-Yong Du, Ming-Jun Wang, and Hong Li
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Rationale, aims and objectives: To determine current practices, attitudes and demands of Chinese anesthesiologists regarding depth of anesthesia (DoA) monitoring. Method: An anonymous online survey was randomly distributed to 8,556 anesthesiologists. Results: The overall response rate was 47% (4,037 respondents). Only 9.1% (95% confidence interval, 8.2%-10.0%) of the respondents routinely use DoA monitors. The main factors influencing the utility of DoA monitors were poor anti-interference ability (56.0%, 54.5%-57.6%), inability to bill insurance or high cost (55.5%, 54.0%-57.0%), limited accuracy (47.9%, 46.3%-49.4%) and inability to monitor analgesic effects (35.7%, 34.2%-37.1%). Academic respondents (91.5%, 90.3%-92.7%) primarily used DoA monitoring to prevent awareness, whereas nonacademic respondents (88.8%, 87.4%-90.2%) primarily used DoA monitoring to guide the delivery of anesthetics. In total, the respondents who had not used a DoA monitor and experienced awareness (61.7%, 57.8%-65.6%) was significantly greater than those who had used one or several DoA monitors (51.5%, 49.8%-53.2%). However, most respondents (95.7%, 95.1%-96.3%) demanded improvements in the accuracy of the monitors for DoA monitoring and a broad application in patients of all ages (86.3%, 85.2%-87.4%), analgesia monitoring (80.4%, 79.2%-81.6%) and all types of anesthetics (75.6%, 74.3%-76.9%). In total, 65.0% (63.6%-66.5%) of the respondents believed that DoA monitors should be combined with EEG and vital sign monitoring, and 53.7% (52.1%-55.2%) believed that advanced DoA monitors should include artificial intelligence. Conclusions: Academic anesthesiologists primarily use DoA monitoring to help prevent awareness, whereas nonacademic anesthesiologists use DoA monitoring to guide the delivery of anesthetics. Regardless of whether a DoA monitor is used, anesthesiologists believe that DoA monitoring could help to reduce the incidence of awareness. Anesthesiologists demand high-accuracy DoA monitors incorporating signals of EEG, multiple vital signs and antinociceptive indicators. DoA monitors with artificial intelligence may represent a new direction for future research on DoA monitoring.
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- 2022
6. Polymorphism of prion protein gene (PRNP) in Nigerian sheep
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Adeniyi C. Adeola, Semiu F. Bello, Abdussamad M. Abdussamad, Akanbi I. Mark, Oscar J. Sanke, Anyebe B. Onoja, Lotanna M. Nneji, Nasiru Abdullahi, Sunday C. Olaogun, Lawal D. Rogo, Godwin F. Mangbon, Shamsudeen L. Pedro, Manasseh P. Hiinan, Muhammad M. Mukhtar, Jebi Ibrahim, Hayatu Saidu, Philip M. Dawuda, Rukayya K. Bala, Hadiza L. Abdullahi, Adebowale E. Salako, Samia Kdidi, Mohamed Habib Yahyaoui, and Ting-Ting Yin
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Nigeria ,polymorphism ,prion protein gene ,scrapie ,sheep ,susceptibility ,Neurology. Diseases of the nervous system ,RC346-429 ,Biology (General) ,QH301-705.5 - Abstract
ABSTRACTPolymorphism of the prion protein gene (PRNP) gene determines an animal’s susceptibility to scrapie. Three polymorphisms at codons 136, 154, and 171 have been linked to classical scrapie susceptibility, although many variants of PRNP have been reported. However, no study has investigated scrapie susceptibility in Nigerian sheep from the drier agro-climate zones. In this study, we aimed to identify PRNP polymorphism in nucleotide sequences of 126 Nigerian sheep by comparing them with public available studies on scrapie-affected sheep. Further, we deployed Polyphen-2, PROVEAN, and AMYCO analyses to determine the structure changes produced by the non-synonymous SNPs. Nineteen (19) SNPs were found in Nigerian sheep with 14 being non-synonymous. Interestingly, one novel SNP (T718C) was identified. There was a significant difference (P
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- 2023
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7. Betaine-assisted recombinase polymerase assay for rapid hepatitis B virus detection
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Ting‐ting Yi, Guo‐zhong Gong, Yan Cai, Han‐yun Zhang, and Hua Liang
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Hepatitis B virus ,Biomedical Engineering ,Recombinase Polymerase Amplification ,Bioengineering ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Recombinases ,chemistry.chemical_compound ,Betaine ,Drug Discovery ,medicine ,Recombinase ,Humans ,recombinase polymerase amplification ,Pathogen ,Polymerase ,biology ,Chemistry ,Process Chemistry and Technology ,lateral flow assay ,General Medicine ,Original Articles ,Hepatitis B ,medicine.disease ,Virology ,biology.protein ,Molecular Medicine ,Original Article ,Nucleic Acid Amplification Techniques ,Biotechnology - Abstract
Hepatitis B virus (HBV) is a worldwide epidemic pathogen that causes hepatitis B. On‐site screening the HBV infection is of critical importance for preventing and diagnosing HBV infection. In this paper, a simple, visual, and rapid method for on‐site detection of HBV‐DNA has been developed. This method is based on betaine‐assisted recombinase polymerase assay and followed with naked‐eye detection via lateral flow assay (BRPA‐LF). Result show that nonspecific amplification is prone to occur in recombinase polymerase amplification (RPA) if the assay was performed with serum sample without purification. This problem has been addressed by adding 0.8 M of betaine to the RPA reactions. It was demonstrated that BRPA‐LF can detect 1,000 copies of HBV‐DNA in 50 μL mixture, and achieved 90% sensitivity and 100% specificity for serum sample detection. These results demonstrated that BRPA‐LF can resist serum interference and has great potential for on‐site screening of HBV infection.
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- 2020
8. Comparative study on clinicopathological features and prognosis of IgA vasculitis nephritis and IgA nephropathy in children
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Yan Lv, Rui Fu, Xiao-Jie Peng, Ying Wang, Ting-Ting Yin, and Yan-Qing Deng
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IgA vasculitis nephritis ,IgA nephropathy ,SQC ,Kidney prognosis ,Children ,Pediatrics ,RJ1-570 - Abstract
Abstract Background IgA vasculitis nephritis (IgAVN) and IgA nephropathy (IgAN) share several clinical and pathological characteristics, though distinctions also exist. Their interrelation, however, remains undefined. This study investigates the clinicopathological divergences and prognostic disparities in pediatric patients with IgAVN and IgAN. Methods Our study encompasses 809 pediatric patients with IgAVN and 236 with IgAN, all of whom underwent kidney biopsy. We utilized the Semiquantitative Classification (SQC) scoring system to juxtapose the pathologies of the two conditions, and performed a COX regression analysis to examine factors influencing their prognoses. Results Both patient groups demonstrated a predominance of males. A seasonality was observed, with a higher incidence of IgAN in the summer, and IgAVN in the fall (P
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- 2023
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9. Germline gene fusions across species reveal the chromosomal instability regions and cancer susceptibility
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Bo-Wen Zhou, Qing-Qin Wu, David H. Mauki, Xuan Wang, Shu-Run Zhang, Ting-Ting Yin, Fang-Liang Chen, Chao Li, Yan-Hu Liu, Guo-Dong Wang, and Ya-Ping Zhang
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Biological sciences ,Genetics ,Cancer systems biology ,Science - Abstract
Summary: The canine transmissible venereal tumor (CTVT) is a clonal cell-mediated cancer with a long evolutionary history and extensive karyotype rearrangements in its genome. However, little is known about its genetic similarity to human tumors. Here, using multi-omics data we identified 11 germline gene fusions (GGFs) in CTVT, which showed higher genetic susceptibility than others. Additionally, we illustrate a mechanism of a complex gene fusion of three gene segments (HSD17B4-DMXL1-TNFAIP8) that we refer to “greedy fusion”. Our findings also provided evidence that expressions of GGFs are downregulated during the tumor regressive phase, which is associated with DNA methylation level. This study presents a comprehensive landscape of gene fusions (GFs) in CTVT, which offers a valuable genetic resource for exploring potential genetic mechanisms underlying the development of cancers in both dogs and humans.
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- 2023
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10. Identifying driving factors of urban land expansion using Google Earth Engine and machine-learning approaches in Mentougou District, China
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Lin-Lin Cheng, Chao Tian, and Ting-Ting Yin
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Medicine ,Science - Abstract
Abstract The research on driving mechanisms of urban land expansion is hot topic of land science. However, the relative importance of anthropogenic-natural factors and how they affect urban land expansion change are still unclear. Based on the Google Earth Engine platform, this study used the support vector machine classifier to extract land-use datasets of Mentougou district of Beijing, China from 1990 to 2016. Supported by machine-learning approaches, multiple linear regression (MLR) and random forests (RF) were applied and compared to identify the influential factors and their relative importance on urban land expansion. The results show: There was a continuous growth in urban land expansion from 1990 to 2016, the increased area reached 6097.42 ha with an average annual rate of 8.01% and average annual intensity rate of 2.57%, respectively. Factors such as elevation, risk of goaf collapse, accessibility, local fiscal expenditure, industrial restructuring, per capita income in rural area, GDP were important drivers of urban land expansion change. The model comparison indicated that RF had greater ability than MLR to identify the non-linear relationships between urban land expansion and explanatory variables. The influencing factors of urban land expansion should be comprehensively considered to regulate new land policy actions in Mentougou.
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- 2022
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11. Identifying cellular mechanisms of zinc-induced relaxation in isolated cardiomyocytes
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Ting-Ting Yi, Jonathan S. Vick, Bradley M. Palmer, Kelly J. Begin, Marc J H Vecchio, Rona J. Delay, and Stephen P. Bell
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inorganic chemicals ,Male ,Sarcomeres ,medicine.medical_specialty ,Patch-Clamp Techniques ,Calcium Channels, L-Type ,Physiology ,Muscle Relaxation ,chemistry.chemical_element ,Zinc ,Rats, Inbred WKY ,Sarcomere ,Sarcoplasmic Reticulum Calcium-Transporting ATPases ,Rats, Sprague-Dawley ,Mice ,Physiology (medical) ,Internal medicine ,medicine ,Animals ,Myocyte ,Myocytes, Cardiac ,Diastolic function ,Dose-Response Relationship, Drug ,Cardiac Excitation and Contraction ,Chemistry ,Ryanodine receptor ,Ryanodine Receptor Calcium Release Channel ,Rats ,Mice, Inbred C57BL ,Endocrinology ,Models, Animal ,Biophysics ,Relaxation (physics) ,Calcium ,Cardiology and Cardiovascular Medicine - Abstract
We tested several molecular and cellular mechanisms of cardiomyocyte contraction-relaxation function that could account for the reduced systolic and enhanced diastolic function observed with exposure to extracellular Zn2+. Contraction-relaxation function was monitored in isolated rat and mouse cardiomyocytes maintained at 37°C, stimulated at 2 or 6 Hz, and exposed to 32 μM Zn2+ or vehicle. Intracellular Zn2+ detected using FluoZin-3 rose to a concentration of ∼13 nM in 3–5 min. Peak sarcomere shortening was significantly reduced and diastolic sarcomere length was elongated after Zn2+ exposure. Peak intracellular Ca2+ detected by Fura-2FF was reduced after Zn2+ exposure. However, the rate of cytosolic Ca2+ decline reflecting sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA2a) activity and the rate of Na+/Ca2+ exchanger activity evaluated by rapid Na+-induced Ca2+ efflux were unchanged by Zn2+ exposure. SR Ca2+ load evaluated by rapid caffeine exposure was reduced by ∼50%, and L-type calcium channel inward current measured by whole cell patch clamp was reduced by ∼70% in cardiomyocytes exposed to Zn2+. Furthermore, ryanodine receptor (RyR) S2808 and phospholamban (PLB) S16/T17 were markedly dephosphorylated after perfusing hearts with 50 μM Zn2+. Maximum tension development and thin-filament Ca2+ sensitivity in chemically skinned cardiac muscle strips were not affected by Zn2+ exposure. These findings suggest that Zn2+ suppresses cardiomyocyte systolic function and enhances relaxation function by lowering systolic and diastolic intracellular Ca2+ concentrations due to a combination of competitive inhibition of Ca2+ influx through the L-type calcium channel, reduction of SR Ca2+ load resulting from phospholamban dephosphorylation, and lowered SR Ca2+ leak via RyR dephosphorylation. The use of the low-Ca2+-affinity Fura-2FF likely prevented the detection of changes in diastolic Ca2+ and SERCA2a function. Other strategies to detect diastolic Ca2+ in the presence of Zn2+ are essential for future work.
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- 2013
12. Increased apoptosis in human knee osteoarthritis cartilage related to the expression of protein kinase B and protein kinase Cα in chondrocytes
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Qingzhi Chen, Ting-ting Yi, Bing Zhang, and Chun Xia
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Programmed cell death ,Histology ,Cartilage ,General Medicine ,Osteoarthritis ,Biology ,medicine.disease ,Protein expression ,Chondrocyte ,Pathology and Forensic Medicine ,Cell biology ,medicine.anatomical_structure ,Apoptosis ,Immunology ,medicine ,Protein kinase A ,Protein kinase B - Abstract
Natural Science Foundation of Fujian, China [C0910644]; National Natural Science Foundation of China [81072015]; Natural Science Foundation of Xiamen, China [3502Z20084015]
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- 2012
13. Ornithoctonus huwenna spider silk protein attenuating diameter and enhancing strength of the electrospun PLLA fiber
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Ting-ting Yi, Min Zhang, Ai-li Zhang, Ye-mei Zhang, Zhi-juan Pan, Lu Zhang, and Wei Wu
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Crystallinity ,Materials science ,Morphology (linguistics) ,Polymers and Plastics ,Spidroin ,Composite number ,Spider silk ,Fiber ,Composite material ,Electrospinning ,Tensile testing - Abstract
Spider silk and poly-L-lactic acid (PLLA) are both good bio-medical materials. PLLA/spidroin composite nano-fibers were obtained by electrospinning with the mixed solution of Ornithoctonus huwenna spider silk/1,1,1,3,3,3,-hexafluoro-2-propanol (HFIP) and PLLA/HFIP. The morphology, molecular conformations, crystallinity degree and mechanical property of the electrospun fibers were investigated by means of SEM, FT-IR, XRD, and tensile test. The results indicated that spider silk protein attenuated the fiber diameter and enhanced the mechanical property of the electrospun PLLA fibers. The diameter of electrospun PLLA fibers were micron level (>500 nm), while with the addition of spider silk protein, the diameter of the PLLA/spidroin composite fibers decreased significantly, reaching nano-scale ( 300 nm) and fine fibers (
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- 2010
14. Genome-wide investigations reveal the population structure and selection signatures of Nigerian cattle adaptation in the sub-Saharan tropics
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David H. Mauki, Abdulfatai Tijjani, Cheng Ma, Said I. Ng’ang’a, Akanbi I. Mark, Oscar J. Sanke, Abdussamad M. Abdussamad, Sunday C. Olaogun, Jebi Ibrahim, Philip M. Dawuda, Godwin F. Mangbon, Rudovick R. Kazwala, Paul S. Gwakisa, Ting-Ting Yin, Yan Li, Min-Sheng Peng, Adeniyi C. Adeola, and Ya-Ping Zhang
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Cattle ,Genotyping-by-sequencing ,Genome ,Convergent evolution ,Africa ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Cattle are considered to be the most desirable livestock by small scale farmers. In Africa, although comprehensive genomic studies have been carried out on cattle, the genetic variations in indigenous cattle from Nigeria have not been fully explored. In this study, genome-wide analysis based on genotyping-by-sequencing (GBS) of 193 Nigerian cattle was used to reveal new insights on the history of West African cattle and their adaptation to the tropical African environment, particularly in sub-Saharan region. Results The GBS data were evaluated against whole-genome sequencing (WGS) data and high rate of variant concordance between the two platforms was evident with high correlated genetic distance matrices genotyped by both methods suggestive of the reliability of GBS applicability in population genetics. The genetic structure of Nigerian cattle was observed to be homogenous and unique from other African cattle populations. Selection analysis for the genomic regions harboring imprints of adaptation revealed genes associated with immune responses, growth and reproduction, efficiency of feeds utilization, and heat tolerance. Our findings depict potential convergent adaptation between African cattle, dogs and humans with adaptive genes SPRY2 and ITGB1BP1 possibly involved in common physiological activities. Conclusion The study presents unique genetic patterns of Nigerian cattle which provide new insights on the history of cattle in West Africa based on their population structure and the possibility of parallel adaptation between African cattle, dogs and humans in Africa which require further investigations.
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- 2022
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15. Chromatin accessibility analysis identifies the transcription factor ETV5 as a suppressor of adipose tissue macrophage activation in obesity
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Ren-Dong Hu, Wen Zhang, Liang Li, Zu-Qi Zuo, Min Ma, Jin-Fen Ma, Ting-Ting Yin, Cai-Yue Gao, Shu-Han Yang, Zhi-Bin Zhao, Zi-Jun Li, Gui-Bin Qiao, Zhe-Xiong Lian, and Kun Qu
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Cytology ,QH573-671 - Abstract
Abstract Activation of adipose tissue macrophages (ATMs) contributes to chronic inflammation and insulin resistance in obesity. However, the transcriptional regulatory machinery involved in ATM activation during the development of obesity is not fully understood. Here, we profiled the chromatin accessibility of blood monocytes and ATMs from obese and lean mice using assay for transposase-accessible chromatin sequencing (ATAC-seq). We found that monocytes and ATMs from obese and lean mice exhibited distinct chromatin accessibility status. There are distinct regulatory elements that are specifically associated with monocyte or ATM activation in obesity. We also discovered several transcription factors that may regulate monocyte and ATM activation in obese mice, specifically a predicted transcription factor named ETS translocation variant 5 (ETV5). The expression of ETV5 was significantly decreased in ATMs from obese mice and its downregulation was mediated by palmitate stimulation. The decrease in ETV5 expression resulted in macrophage activation. Our results also indicate that ETV5 suppresses endoplasmic reticulum (ER) stress and Il6 expression in macrophages. Our work delineates the changes in chromatin accessibility in monocytes and ATMs during obesity, and identifies ETV5 as a critical transcription factor suppressing ATM activation, suggesting its potential use as a therapeutic target in obesity-related chronic inflammation.
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- 2021
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16. [Oxidative mechanism of uric acid induced CRP expression in human umbilical vein endothelial cells]
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Li-Jun, Du, Jing-Guo, Zhou, Xing-Jun, Ren, Ting-Ting, Yi, and Xing-Liang, Jiang
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Oxidative Stress ,C-Reactive Protein ,Human Umbilical Vein Endothelial Cells ,Humans ,Reactive Oxygen Species ,Cells, Cultured ,Uric Acid - Abstract
To explore the oxidative mechanism of uric acid (UA) induced CRP expression in human umbilical vein endothelial cells.Different concentrations of UA (0 mg/dL, 4 mg/dL, 8 mg/dL, 12 mg/dL, 16 mg/dl) were incubated 12 h with HUVECs, and HUVECs were stimulated with 12 mg/dl. UA for different times (6 h, 12 h, 24 h, 48 h). CRP mRNA and protein expression were determined by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot, respectively; the effects of uric acid on the intracellular reactive oxygen species (ROS) production in HUVECs were measured by fluorescence microscope and flow cytometric analysis using a 2', 7'-Dichlorofluorescin diacetate (DCF-DA) fluorescence probe. The effects of N-acetyl cysteine (NAC) on UA-induced levels of ROS, mRNA and protein of CRP in HUVECs were also observed.The results demonstrated that UA could significantly increase the mRNA and protein expression of CRP in HUVECs in time- and concentration-dependent manners. HUVECs were stimulated with 12 mg/dL UA at 6 h, mRNA and protein levels of CRP significantly higher than that of control level (P0.05), reached a peak at 12 h (P0. 01). NAC reduced UA-induced levels of ROS, mRNA and protein of CRP in HUVECs compared with those of 12 mg/dL UA induced group(P0. 05).Uric acid significantly increased mRNA and protein expression of CRP in HUVECs in time- and concentration-dependent manners. Its mechanism may be associated with uric acid induced increasing of ROS levels in endothelial cells, which suggested that the uric acid mediated oxidative stress and inflammation may be involved in the injury of endothelial cells.
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- 2013
17. Lactate score predicts survival, immune cell infiltration and response to immunotherapy in breast cancer
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Ting-Ting Yin, Meng-Xing Huang, Fei Wang, Yi-Hua Jiang, Jie Long, Liang Li, and Jie Cao
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breast cancer ,lactate score ,tumor microenvironment ,immunotherapy ,chemotherapy ,Genetics ,QH426-470 - Abstract
Background: Tumor-derived lactate can modulate the function of infiltrating immune cells to establish an immunosuppressive microenvironment that favors tumor progression. However, possible effects of lactate-related genes (LRGs) on the tumor microenvironment (TME) of breast cancer (BRCA) are still unknown.Methods: LRGs were comprehensively screened from lactate metabolism-related pathways. We correlated the expression of these LRGs with immune cell infiltrating characteristics in the TME and clinicopathological features of patients. We also established a lactate score for quantifying lactate metabolism patterns of cancers and to predict of recurrence-free survival (RFS).Results: We successfully constructed a lactate score that was an independent prognostic factor in BRCA. A low lactate score, which was associated with immune activation with increased CD8+ T cells infiltration levels, indicated an inflamed TME. Consistently, higher expression levels of inhibitory immune checkpoints, including PD-L1, LAG3, CTLA4, and TIM3, as observed from high lactate score subgroup, suggested an immune-desert phenotype as well as poor prognosis. Moreover, a low lactate score predicted the increased chemotherapeutic drug sensitivity and enhanced anti-PD-1 immunotherapy responses.Conclusion: The present study analyzed the potential roles of LRGs in the TME diversity and prognosis. These results will help to improve our understanding of the characteristics of TME immune cell infiltration and guide the development of more effective immunotherapy strategies.
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- 2022
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18. Large-scale genomic analysis reveals the genetic cost of chicken domestication
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Ming-Shan Wang, Jin-Jin Zhang, Xing Guo, Ming Li, Rachel Meyer, Hidayat Ashari, Zhu-Qing Zheng, Sheng Wang, Min-Sheng Peng, Yu Jiang, Mukesh Thakur, Chatmongkon Suwannapoom, Ali Esmailizadeh, Nalini Yasoda Hirimuthugoda, Moch Syamsul Arifin Zein, Szilvia Kusza, Hamed Kharrati-Koopaee, Lin Zeng, Yun-Mei Wang, Ting-Ting Yin, Min-Min Yang, Ming-Li Li, Xue-Mei Lu, Emiliano Lasagna, Simone Ceccobelli, Humpita Gamaralalage Thilini Nisanka Gunwardana, Thilina Madusanka Senasig, Shao-Hong Feng, Hao Zhang, Abul Kashem Fazlul Haque Bhuiyan, Muhammad Sajjad Khan, Gamamada Liyanage Lalanie Pradeepa Silva, Le Thi Thuy, Okeyo A. Mwai, Mohamed Nawaz Mohamed Ibrahim, Guojie Zhang, Kai-Xing Qu, Olivier Hanotte, Beth Shapiro, Mirte Bosse, Dong-Dong Wu, Jian-Lin Han, and Ya-Ping Zhang
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Domestication ,Bottleneck ,Genetic load ,Deleterious mutation ,Domestic chicken ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Species domestication is generally characterized by the exploitation of high-impact mutations through processes that involve complex shifting demographics of domesticated species. These include not only inbreeding and artificial selection that may lead to the emergence of evolutionary bottlenecks, but also post-divergence gene flow and introgression. Although domestication potentially affects the occurrence of both desired and undesired mutations, the way wild relatives of domesticated species evolve and how expensive the genetic cost underlying domestication is remain poorly understood. Here, we investigated the demographic history and genetic load of chicken domestication. Results We analyzed a dataset comprising over 800 whole genomes from both indigenous chickens and wild jungle fowls. We show that despite having a higher genetic diversity than their wild counterparts (average π, 0.00326 vs. 0.00316), the red jungle fowls, the present-day domestic chickens experienced a dramatic population size decline during their early domestication. Our analyses suggest that the concomitant bottleneck induced 2.95% more deleterious mutations across chicken genomes compared with red jungle fowls, supporting the “cost of domestication” hypothesis. Particularly, we find that 62.4% of deleterious SNPs in domestic chickens are maintained in heterozygous states and masked as recessive alleles, challenging the power of modern breeding programs to effectively eliminate these genetic loads. Finally, we suggest that positive selection decreases the incidence but increases the frequency of deleterious SNPs in domestic chicken genomes. Conclusion This study reveals a new landscape of demographic history and genomic changes associated with chicken domestication and provides insight into the evolutionary genomic profiles of domesticated animals managed under modern human selection.
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- 2021
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19. [Effects of PTHrP and Notch signaling on the proliferation of epiphysis stem cells]
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Fei-tai, Lin, Chun, Xia, Bing, Zhang, Jia-gu, Huang, Xin-peng, Zheng, Ting-ting, Yi, Hong-hai, Zhao, and Yi-bei, Zhang
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Rats, Sprague-Dawley ,Receptors, Notch ,Stem Cells ,Parathyroid Hormone-Related Protein ,Animals ,Epiphyses ,Cells, Cultured ,Cell Proliferation ,Rats ,Signal Transduction - Abstract
To study the regulation of the proliferation of epiphysis stem cells by the PTHrP (parathyroid hormone related peptide) and Notch signaling systems.An organ culture system of femurs of SD rat in 24 h after birth was employed. PTHrP (1 - 34) was used as the activator of the PTHrP signaling pathway and PTHrP (7 - 34) as the antagonist of PTH (parathyroid hormone)-receptor. For Notch signaling system, Jagged1/Fc was used as the activator and DAPT as its inhibitor. The femurs were cultured in DMEM (Dulbecco's modified Eagle's medium)/F12 medium while phosphate buffered saline was used for the control groups. Hematoxylin and eosin staining and bromodeoxyuridine analysis were used to analyze the length of the epiphysis stem cells zone and the proliferation of epiphysis stem cells. The expression of NICD (Notch intra-cellular domain) and Jagged1 were analyzed by immunohistochemistry. The epiphysis stem cells were transfected with the lentiviral vectors with rat PTHrP gene overexpression or inhibition properties, the cells transfected with the PGC-GFP-lentivirus or NC-GFP-lentivirus were used as control. Western blot was employed to detect the expression of NICD and Jagged1 genes.PTHrP (1 - 34) and Jagged1/Fc could dramatically elevate the rate of epiphysis stem cells zone by the whole growth plate length measurement while PTHrP (7 - 34) and DAPT could decrease the rate. Brdu analysis also showed that the number of proliferative epiphysis stem cells could be up-regulated by the PTHrP (1 - 34) or Jagged1/Fc signaling. By contrast, the treatment with PTHrP (7 - 34) or DAPT reduced the number of proliferative epiphysis stem cells. Immunohistochemistry and Western blot showed a significantly elevated expression of NICD and Jagged1 when PTHrP signaling was activated while a reductive expression of NICD and Jagged1 when PTHrP signaling was inactivated.Both of PTHrP and Notch signaling system could promote the proliferation of epiphysis stem cells. And the PTHrP signaling can stimulate Notch signaling to promote the proliferation of epiphysis stem cells.
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- 2011
20. 17β-Estradiol promotes cell proliferation in rat osteoarthritis model chondrocytes via PI3K/Akt pathway
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Xiao Yong Wang, Gang Song, Jia Gu Huang, Bing Zhang, Ting Ting Yi, Chun Xia, and Xin Peng Zheng
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medicine.medical_specialty ,Cell Survival ,Short Communication ,Blotting, Western ,Interleukin-1beta ,Gene Expression ,Biology ,Biochemistry ,Chondrocyte ,Phosphatidylinositol 3-Kinases ,Chondrocytes ,Internal medicine ,Osteoarthritis ,medicine ,Estrogen receptor ,Animals ,Phosphorylation ,Molecular Biology ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Cell proliferation ,Cells, Cultured ,Estradiol ,Cell growth ,Cartilage ,Akt ,17β-estradiol ,Cell Biology ,Molecular medicine ,Rats ,Disease Models, Animal ,medicine.anatomical_structure ,Endocrinology ,Apoptosis ,PI3K/Akt pathway ,Cancer research ,Rat osteoarthritis model chondrocyte ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
Osteoarthritis (OA) is the most common cause of musculoskeletal pain and disability. The importance of chondrocytes in the pathogenesis of OA is unequivocal. 17β-estradiol (E2) has a potential protective effect against OA. However, the mechanism of E2 in OA chondrocytes remains unclear. In this study, we investigated the regulative effect of E2 on cell growth and the relationship between E2 and the PI3K/Akt pathway in rat OA model chondrocytes (pretreated with interleukin-1β). We found that E2 induced chondrocyte proliferation, and increased the expression level of Akt simultaneously, especially the expression level of P-Akt. Furthermore, the inhibition of P-Akt could block chondrocyte proliferation induced by E2. These results suggest that PI3K/Akt activation induced by E2 may be an important factor in the mechanism of E2 in cell proliferation in rat OA model chondrocytes, and help further understanding the role of E2 in OA progression.
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- 2011
21. Moving horizon based wavelet de-noising method of dual-observed geomagnetic signal for nonlinear high spin projectile roll positioning
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Ting-ting Yin, Fang-xiu Jia, and Xiao-ming Wang
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High-spin projectile roll positioning ,Dual-observed geomagnetic signal ,Wavelet de-noising ,Discrete wavelet transform ,Military Science - Abstract
Phase-frequency characteristics of approximate sinusoidal geomagnetic signals can be used for projectile roll positioning and other high-precision trajectory correction applications. The sinusoidal geomagnetic signal deforms in the exposed and magnetically contaminated environment. In order to preciously recognize the roll information and effectively separate the noise component from the original geomagnetic sequence, based on the error source analysis, we propose a moving horizon based wavelet de-noising method for the dual-observed geomagnetic signal filtering where the captured rough roll frequency value provides reasonable wavelet decomposition and reconstruction level selection basis for sampled sequence; a moving horizon window guarantees real-time performance and non-cumulative calculation amount. The complete geomagnetic data in full ballistic range and three intercepted paragraphs are used for performance assessment. The positioning performance of the moving horizon wavelet de-noising method is compared with the band-pass filter. The results show that both noise reduction techniques improve the positioning accuracy while the wavelet de-noising method is always better than the band-pass filter. These results suggest that the proposed moving horizon based wavelet de-noising method of the dual-observed geomagnetic signal is more applicable for various launch conditions with better positioning performance.
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- 2020
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22. Genomic regions under selection in the feralization of the dingoes
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Shao-jie Zhang, Guo-Dong Wang, Pengcheng Ma, Liang-liang Zhang, Ting-Ting Yin, Yan-hu Liu, Newton O. Otecko, Meng Wang, Ya-ping Ma, Lu Wang, Bingyu Mao, Peter Savolainen, and Ya-ping Zhang
- Subjects
Science - Abstract
Dingoes evolved in isolation from both their domesticated and wild ancestors. Here, the authors investigate the genomic basis of the feralization of dingoes and trace their origin to domestic dogs that migrated to Australia approximately 8300 years ago.
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- 2020
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23. Effects of liraglutide, metformin and gliclazide on body composition in patients with both type 2 diabetes and non‐alcoholic fatty liver disease: A randomized trial
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Wen‐Huan Feng, Yan Bi, Ping Li, Ting‐Ting Yin, Cai‐Xia Gao, Shan‐Mei Shen, Li‐Jun Gao, Dong‐Hui Yang, and Da‐Long Zhu
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Antidiabetic agents ,Body composition ,Type 2 diabetes mellitus ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Abstract Aims/Introduction To compare the effects of gliclazide, liraglutide and metformin on body composition in patients with type 2 diabetes mellitus with non‐alcoholic fatty liver disease. Materials and Methods A total of 85 patients were randomly allocated to receive gliclazide (n = 27), liraglutide (n = 29) or metformin (n = 29) monotherapy for 24 weeks. Body composition was measured using dual‐energy X‐ray absorptiometry. Results Liraglutide and metformin reduced total, trunk, limb, android and gynoid fat mass; this also led to weight reduction. However, gliclazide treatment produced no significant changes in weight or fat mass, likely because reductions in fat mass were concomitant with increases in lean tissue mass. Blood glucose concentrations and glycated hemoglobin levels improved in all treatment arms; levels of the latter were lower in patients treated with liraglutide and metformin. Serum alanine aminotransferase concentrations decreased in all treatment arms, whereas serum aspartate aminotransferase concentrations were reduced only by liraglutide and metformin. In all patients, weight loss and total, trunk, limb, and android fat mass reductions were positively correlated with decreases in serum alanine aminotransferase and aspartate aminotransferase levels, whereas reductions in waist circumference were positively correlated with lower serum alanine aminotransferase levels. Conclusions Compared with gliclazide, liraglutide and metformin monotherapies result in greater weight loss, reductions in body fat mass, and better blood glucose control among type 2 diabetes mellitus patients with non‐alcoholic fatty liver disease. Reductions in weight, fat mass and waist circumference favorably affect hepatic function.
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- 2019
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24. A high-throughput SNP discovery strategy for RNA-seq data
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Yun Zhao, Ke Wang, Wen-li Wang, Ting-ting Yin, Wei-qi Dong, and Chang-jie Xu
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Single nucleotide polymorphism (SNP) ,RNA-seq ,Paired-end read length ,Trinity ,GATK ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Single nucleotide polymorphisms (SNP) have been applied as important molecular markers in genetics and breeding studies. The rapid advance of next generation sequencing (NGS) provides a high-throughput means of SNP discovery. However, SNP development is limited by the availability of reliable SNP discovery methods. Especially, the optimum assembler and SNP caller for accurate SNP prediction from next generation sequencing data are not known. Results Herein we performed SNP prediction based on RNA-seq data of peach and mandarin peel tissue under a comprehensive comparison of two paired-end read lengths (125 bp and 150 bp), five assemblers (Trinity, IDBA, oases, SOAPdenovo, Trans-abyss) and two SNP callers (GATK and GBS). The predicted SNPs were compared with the authentic SNPs identified via PCR amplification followed by gene cloning and sequencing procedures. A total of 40 and 240 authentic SNPs were presented in five anthocyanin biosynthesis related genes in peach and in nine carotenogenic genes in mandarin. Putative SNPs predicted from the same RNA-seq data with different strategies led to quite divergent results. The rate of false positive SNPs was significantly lower when the paired-end read length was 150 bp compared with 125 bp. Trinity was superior to the other four assemblers and GATK was substantially superior to GBS due to a low rate of missing authentic SNPs. The combination of assembler Trinity, SNP caller GATK, and the paired-end read length 150 bp had the best performance in SNP discovery with 100% accuracy both in peach and in mandarin cases. This strategy was applied to the characterization of SNPs in peach and mandarin transcriptomes. Conclusions Through comparison of authentic SNPs obtained by PCR cloning strategy and putative SNPs predicted from different combinations of five assemblers, two SNP callers, and two paired-end read lengths, we provided a reliable and efficient strategy, Trinity-GATK with 150 bp paired-end read length, for SNP discovery from RNA-seq data. This strategy discovered SNP at 100% accuracy in peach and mandarin cases and might be applicable to a wide range of plants and other organisms.
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- 2019
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25. Effects of exenatide versus insulin glargine on body composition in overweight and obese T2DM patients: a randomized controlled trial
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Ting-Ting Yin, Yan Bi, Ping Li, Shan-Mei Shen, Wei-Min Wang, Can Jiang, Cai-Xia Gao, Yan Wang, Li-Jun Gao, Da-Long Zhu, and Wen-Huan Feng
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Body composition ,Exenatide ,Insulin glargine ,Type 2 diabetes ,Nutrition. Foods and food supply ,TX341-641 ,Nutritional diseases. Deficiency diseases ,RC620-627 - Abstract
Abstract Background Weight loss, especially fat mass reduction, helps to improve blood glucose control, insulin sensitivity, and β-cell function. This study aimed to compare the effect of exenatide and glargine on body composition in overweight and obese patients with type 2 diabetes (T2DM) who do not achieve adequate glycemic control with metformin. Methods We performed a prospective, randomized study of 37 overweight or obese patients with T2DM who had inadequate glycemic control with metformin. The patients were treated with either exenatide or glargine for 16 weeks. Dual-energy X-ray absorptiometry was used to assess body composition. Results Post-intervention weight, body mass index (BMI), waist circumference, body mass, and fat mass were lower in patients treated with exenatide, while weight and BMI significantly increased with glargine. Reductions in weight, BMI, body fat mass, and percent fat mass (except for gynoid) were greater with exenatide than with glargine, and percent lean tissue (other than the limbs) increased with exenatide. In all body regions except for the limbs, fat mass decreased with exenatide to a greater extent than lean tissue. Glucose control, insulin resistance, and β-cell function were not different between the treatment groups. Conclusions For overweight and obese patients whose T2DM was inadequately controlled with metformin, exenatide and glargine achieved similar improvements in glycemic control, insulin sensitivity, and β-cell function.However, exenatide produced better weight and fat mass reduction, which were beneficial for blood glucose control. Our findings may guide the selection of appropriate drugs for glycemic and weight control. Trial registration NCT02325960, registered 25 December 2014.
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- 2018
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26. Genetic variation of Nigerian cattle inferred from maternal and paternal genetic markers
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David H. Mauki, Adeniyi C. Adeola, Said I. Ng’ang’a, Abdulfatai Tijjani, Ibikunle Mark Akanbi, Oscar J. Sanke, Abdussamad M. Abdussamad, Sunday C. Olaogun, Jebi Ibrahim, Philip M. Dawuda, Godwin F. Mangbon, Paul S. Gwakisa, Ting-Ting Yin, Min-Sheng Peng, and Ya-Ping Zhang
- Subjects
Y-chromosome ,Genetic diversity ,Nigerian cattle ,West Africa ,mtDNA ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
The African cattle provide unique genetic resources shaped up by both diverse tropical environmental conditions and human activities, the assessment of their genetic diversity will shade light on the mechanism of their remarkable adaptive capacities. We therefore analyzed the genetic diversity of cattle samples from Nigeria using both maternal and paternal DNA markers. Nigerian cattle can be assigned to 80 haplotypes based on the mitochondrial DNA (mtDNA) D-loop sequences and haplotype diversity was 0.985 + 0.005. The network showed two major matrilineal clustering: the dominant cluster constituting the Nigerian cattle together with other African cattle while the other clustered Eurasian cattle. Paternal analysis indicates only zebu haplogroup in Nigerian cattle with high genetic diversity 1.000 ± 0.016 compared to other cattle. There was no signal of maternal genetic structure in Nigerian cattle population, which may suggest an extensive genetic intermixing within the country. The absence of Bos indicus maternal signal in Nigerian cattle is attributable to vulnerability bottleneck of mtDNA lineages and concordance with the view of male zebu genetic introgression in African cattle. Our study shades light on the current genetic diversity in Nigerian cattle and population history in West Africa.
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- 2021
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27. Ornithoctonus huwenna spider silk protein attenuating diameter and enhancing strength of the electrospun PLLA fiber.
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Min Zhang, Ting-ting Yi, Ye-mei Zhang, Lu Zhang, Wei Wu, Ai-li Zhang, and Zhi-juan Pan
- Subjects
MILK proteins ,LACTIC acid ,NANOFIBERS ,ELECTROSPINNING ,FOURIER transform infrared spectroscopy - Abstract
Spider silk and poly--lactic acid (PLLA) are both good bio-medical materials. PLLA/spidroin composite nano-fibers were obtained by electrospinning with the mixed solution of Ornithoctonus huwenna spider silk/1,1,1,3,3,3,-hexafluoro-2-propanol (HFIP) and PLLA/HFIP. The morphology, molecular conformations, crystallinity degree and mechanical property of the electrospun fibers were investigated by means of SEM, FT-IR, XRD, and tensile test. The results indicated that spider silk protein attenuated the fiber diameter and enhanced the mechanical property of the electrospun PLLA fibers. The diameter of electrospun PLLA fibers were micron level (>500 nm), while with the addition of spider silk protein, the diameter of the PLLA/spidroin composite fibers decreased significantly, reaching nano-scale (<500 nm), and the diameter of the composite fibers displayed a bipolar distribution of coarse fibers (>300 nm) and fine fibers (<300 nm). The FT-IR and XRD analysis showed that the blend nano-fibers had higher crystallinity, and a phase separation occurred. The mechanical property of the electrospun fiber mat was improved by adding a certain amount of spider silk protein in the PLLA/HFIP solution. When the mass fraction of the spider silk protein was 4%, the breaking strength of the electrospun PLLA/spidroin composite nano-fiber mat was 112% of pure PLLA micro-fiber mat's. Copyright © 2010 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]
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- 2011
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28. Anti-Influenza Serum and Mucosal Antibody Responses After Administration of Live Attenuated or Inactivated Influenza Vaccines to HIV-Infected Children.
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Weinberg, Adriana, Lin-Ye Song, Walker, Robert, Allende, Maria, Fenton, Terence, Patterson-Bartlett, Julie, Nachman, Sharon, Kemble, George, Ting-Ting Yi, Defechereux, Patricia, Wara, Diane, Read, Jenn!fer S., and Levin, Myron
- Published
- 2010
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29. Cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges
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Peng-jun Lu, Chun-yan Wang, Ting-ting Yin, Si-lin Zhong, Don Grierson, Kun-song Chen, and Chang-jie Xu
- Subjects
Medicine ,Science - Abstract
Abstract Ripe Cara Cara sweet orange contains 25 times as much carotenoids in flesh as Newhall sweet orange, due to high accumulation of carotenes, mainly phytoene, lycopene and phytofluene. Only yellow globular chromoplasts were observed in Newhall flesh. Distinct yellow globular and red elongated crystalline chromoplasts were found in Cara Cara but only one type of chromoplast was present in each cell. The red crystalline chromoplasts contained lycopene as a dominant carotenoid and were associated with characteristic carotenoid sequestering structures. The increased accumulation of linear carotenes in Cara Cara is not explained by differences in expression of all 18 carotenogenic genes or gene family members examined, or sequence or abundance of mRNAs from phytoene synthase (PSY) and chromoplast-specific lycopene β-cyclase (CYCB) alleles. 2-(4-Chlorophenylthio)-triethylamine hydrochloride (CPTA) enhanced lycopene accumulation and induced occurrence of red crystalline chromoplasts in cultured Newhall juice vesicles, indicating that carotenoid synthesis and accumulation can directly affect chromoplast differentiation and structure. Norflurazon (NFZ) treatment resulted in high accumulation of phytoene and phytofluene in both oranges, and the biosynthetic activity upstream of phytoene desaturase was similar in Newhall and Cara Cara. Possible mechanisms for high carotene accumulation and unique development of red crystalline chromoplasts in Cara Cara are discussed.
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- 2017
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30. Molecular mechanism of the tree shrew's insensitivity to spiciness.
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Yalan Han, Bowen Li, Ting-Ting Yin, Cheng Xu, Rose Ombati, Lei Luo, Yujie Xia, Lizhen Xu, Jie Zheng, Yaping Zhang, Fan Yang, Guo-Dong Wang, Shilong Yang, and Ren Lai
- Subjects
Biology (General) ,QH301-705.5 - Abstract
Spicy foods elicit a pungent or hot and painful sensation that repels almost all mammals. Here, we observe that the tree shrew (Tupaia belangeri chinensis), which possesses a close relationship with primates and can directly and actively consume spicy plants. Our genomic and functional analyses reveal that a single point mutation in the tree shrew's transient receptor potential vanilloid type-1 (TRPV1) ion channel (tsV1) lowers its sensitivity to capsaicinoids, which enables the unique feeding behavior of tree shrews with regards to pungent plants. We show that strong selection for this residue in tsV1 might be driven by Piper boehmeriaefolium, a spicy plant that geographically overlaps with the tree shrew and produces Cap2, a capsaicin analog, in abundance. We propose that the mutation in tsV1 is a part of evolutionary adaptation that enables the tree shrew to tolerate pungency, thus widening the range of its diet for better survival.
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- 2018
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31. The impact of acceptance of disability and psychological resilience on post-traumatic stress disorders in burn patients
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Zhe-Yuan Xia, Yue Kong, Ting-Ting Yin, Su-Hua Shi, Rong Huang, and Yu-Hong Cheng
- Subjects
Acceptance of disability ,Burns ,Post-traumatic ,Psychological resilience ,Stress disorders ,Nursing ,RT1-120 - Abstract
Objective: To investigate the impact of acceptance of disability and psychological resilience on post-traumatic stress disorders (PTSD) in patients with burns. Methods: A total of 127 patients with burns were asked to complete PTSD Checklist-Civilian Version, Acceptance of Disability Scale and Connor–Davidson Resilience Scale questionnaires. Results were evaluated using correlational and regression analyses. Results: The incidence of PTSD in burn patients was 37.80% (48/127), with an overall average checklist score of 45.78 ± 15.29 points. PTSD was negatively correlated with the level of disability acceptance and psychological resilience (p
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- 2014
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32. Complete mitochondrial genome of Sri Lankan Junglefowl (Gallus lafayetti) and phylogenetic study
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Nalini Yasoda Hirimuthugoda, Adeniyi C. Adeola, Xing Chen, Patthamesthrige Wimal Anthony Perera, Weligalle Wedarallage Dewar Asoka Gunawardena, Humpita Gamaralalage Thilini Nisanka Gunwardana, Ting-Ting Yin, Ming-Shan Wang, Gui-Mei Li, Min-Sheng Peng, and Ya-Ping Zhang
- Subjects
mitochondrial dna ,next-generation sequencing ,phylogenetic analysis ,sri lankan junglefowl ,Genetics ,QH426-470 - Abstract
The complete mitochondrial genomes of two Sri Lankan junglefowl (Gallus lafayetti: CJF) individuals were sequenced by using next-generation sequencing technique. Samples were collected from Rathnapura and Pelmadulla areas in Sri Lanka. The complete mitochondrial DNA is 16,839 bp in length, with a typical mitogenome structure composed of a non-coding control region, 22 tRNA, two rRNA, and 13 protein-coding genes. Overall base composition is 30% A, 23.9% T, 32.3% C, and 13.6% G indicating high content of 54.0% A + T for both individuals. Phylogenetic analysis reveals that CJF samples cluster with the clade of the green junglefowl (Gallus varius) and red junglefowl (Gallus gallus) than to grey junglefowl (Gallus sonerattii: GyJF). This result can be subsequently used to provide essential information for junglefowl evolution.
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- 2018
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33. Mitochondrial genetic diversity, selection and recombination in a canine transmissible cancer
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Andrea Strakova, Máire Ní Leathlobhair, Guo-Dong Wang, Ting-Ting Yin, Ilona Airikkala-Otter, Janice L Allen, Karen M Allum, Leontine Bansse-Issa, Jocelyn L Bisson, Artemio Castillo Domracheva, Karina F de Castro, Anne M Corrigan, Hugh R Cran, Jane T Crawford, Stephen M Cutter, Laura Delgadillo Keenan, Edward M Donelan, Ibikunle A Faramade, Erika Flores Reynoso, Eleni Fotopoulou, Skye N Fruean, Fanny Gallardo-Arrieta, Olga Glebova, Rodrigo F Häfelin Manrique, Joaquim JGP Henriques, Natalia Ignatenko, Debbie Koenig, Marta Lanza-Perea, Remo Lobetti, Adriana M Lopez Quintana, Thibault Losfelt, Gabriele Marino, Inigo Martincorena, Simón Martínez Castañeda, Mayra F Martínez-López, Michael Meyer, Berna Nakanwagi, Andrigo B De Nardi, Winifred Neunzig, Sally J Nixon, Marsden M Onsare, Antonio Ortega-Pacheco, Maria C Peleteiro, Ruth J Pye, John F Reece, Jose Rojas Gutierrez, Haleema Sadia, Sheila K Schmeling, Olga Shamanova, Richard K Ssuna, Audrey E Steenland-Smit, Alla Svitich, Ismail Thoya Ngoka, Bogdan A Vițălaru, Anna P de Vos, Johan P de Vos, Oliver Walkinton, David C Wedge, Alvaro S Wehrle-Martinez, Mirjam G van der Wel, Sophie AE Widdowson, and Elizabeth P Murchison
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Dog ,transmissible cancer ,canine transmissible venereal tumour ,mitochondria ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Canine transmissible venereal tumour (CTVT) is a clonally transmissible cancer that originated approximately 11,000 years ago and affects dogs worldwide. Despite the clonal origin of the CTVT nuclear genome, CTVT mitochondrial genomes (mtDNAs) have been acquired by periodic capture from transient hosts. We sequenced 449 complete mtDNAs from a global population of CTVTs, and show that mtDNA horizontal transfer has occurred at least five times, delineating five tumour clades whose distributions track two millennia of dog global migration. Negative selection has operated to prevent accumulation of deleterious mutations in captured mtDNA, and recombination has caused occasional mtDNA re-assortment. These findings implicate functional mtDNA as a driver of CTVT global metastatic spread, further highlighting the important role of mtDNA in cancer evolution.
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- 2016
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