Author: "Tina Parkner" - Searchworks@Jio Institute Digital Library Search Results

38 results on '"Tina Parkner"'

1. Serum GFAP – reference interval and preanalytical properties in Danish adults

Author: Lea Tybirk, Claus Vinter Bødker Hviid, Cindy Soendersoe Knudsen, and Tina Parkner
Subjects: Adult, Serum, Denmark, Biochemistry (medical), Clinical Biochemistry, General Medicine, stability, glial fibrillary acidic protein (GFAP), Young Adult, Reference Values, Glial Fibrillary Acidic Protein, preanalytical, Humans, reference range, serum, Biomarkers
Abstract: Objectives Glial fibrillary acidic protein (GFAP) is a promising biomarker that could potentially contribute to diagnosis and prognosis in neurological diseases. The biomarker is approaching clinical use but the reference interval for serum GFAP remains to be established, and knowledge about the effect of preanalytical factors is also limited. Methods Serum samples from 371 apparently healthy reference subjects, 21–90 years of age, were measured by a single-molecule array (Simoa) assay. Continuous reference intervals were modelled using non-parametric quantile regression and compared with traditional age-partitioned non-parametric reference intervals established according to the Clinical and Laboratory Standards Institute (CLSI) guideline C28-A3. The following preanalytical conditions were also examined: stability in whole blood at room temperature (RT), stability in serum at RT and −20 °C, repeated freeze-thaw cycles, and haemolysis. Results The continuous reference interval showed good overall agreement with the traditional age-partitioned reference intervals of 25–136 ng/L, 34–242 ng/L, and 5–438 ng/L for the age groups 20–39, 40–64, and 65–90 years, respectively. Both types of reference intervals showed increasing levels and variability of serum GFAP with age. In the preanalytical tests, the mean changes from baseline were 2.3% (95% CI: −2.4%, 6.9%) in whole blood after 9 h at RT, 3.1% (95% CI: −4.5%, 10.7%) in serum after 7 days at RT, 10.4% (95% CI: −6.0%, 26.8%) in serum after 133 days at −20 °C, and 10.4% (95% CI: 9.5%, 11.4%) after three freeze-thaw cycles. Conclusions The study establishes age-dependent reference ranges for serum GFAP in adults and demonstrates overall good stability of the biomarker.
Published: 2022
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2. Serum GFAP - pediatric reference interval in a cohort of Danish children

Author: Lea Tybirk, Claus Vinter Bødker Hviid, Cindy Soendersoe Knudsen, and Tina Parkner
Subjects: children, Biochemistry (medical), Clinical Biochemistry, General Medicine, reference interval, glial fibrillary acidic protein (GFAP), serum
Abstract: Objectives Glial fibrillary acidic protein (GFAP) in blood is an emerging biomarker of brain injury and neurological disease. Its clinical use in children is limited by the lack of a reference interval (RI). Thus, the aim of the present study was to establish an age-dependent continuous RI for serum GFAP in children. Methods Excess serum from routine allergy testing of 391 children, 0.4–17.9 years of age, was measured by a single-molecule array (Simoa) assay. A continuous RI was modelled using non-parametric quantile regression and presented both graphically and tabulated as discrete one-year RIs based on point estimates from the model. Results Serum GFAP showed a strong age-dependency with declining levels and variability from infants to adolescents. The estimated median level decreased 66 % from four months to five years of age and another 65 % from five years to 17.9 years of age. No gender difference was observed. Conclusions The study establishes an age-dependent RI for serum GFAP in children showing high levels and variability in the first years of life.
Published: 2023
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3. Reference intervals for plasma vitamin B12 and plasma/serum methylmalonic acid in Danish children, adults and elderly

Author: Anders Abildgaard, Cindy Soendersoe Knudsen, Carsten Schriver Hoejskov, Eva Greibe, and Tina Parkner
Subjects: Adult, Vitamin B12, Denmark, Biochemistry (medical), Clinical Biochemistry, Reference intervals, food and beverages, nutritional and metabolic diseases, Vitamin B 12 Deficiency, General Medicine, Biochemistry, Methylmalonic acid, Vitamin B 12, Folic Acid, Vitamin B12 deficiency, Reference Values, polycyclic compounds, Humans, Child, Homocysteine, Aged, Methylmalonic Acid
Abstract: Background: Plasma/serum vitamin B12 (B12) is often used to screen for B12 deficiency complemented with analysis of methylmalonic acid (MMA) in case of low B12. The concentration of both analytes likely depends on age, and we, therefore, aimed at establishing 95% age-adjusted reference intervals (RIs) for plasma B12 and serum/plasma MMA in the Danish population. Methods: We collected and analysed blood samples from healthy children, adults, and elderly individuals and extracted routine clinical B12 and MMA results to establish RIs. We also evaluated the association between matching B12 and MMA results. Results: We suggest the following RIs for plasma B12 and plasma/serum MMA, respectively. 0–
Published: 2022
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4. Diagnostic Cut-offs for CSF β-amyloid and tau proteins in a Danish dementia clinic

Author: Anders Abildgaard, Tina Parkner, Cindy Soendersoe Knudsen, Hanne Gottrup, and Henriette Klit
Subjects: Amyloid, Alzheimer Disease, Biochemistry (medical), Clinical Biochemistry, tau Proteins, General Medicine, Biochemistry, Biomarkers, Cerebrospinal Fluid
Abstract: Background: Analysis of beta-amyloid 1–42 (Aβ42), total tau (t-tau) and phosphorylated-tau 181 (p-tau) in the cerebrospinal fluid (CSF) is often performed as a part of the diagnostic work-up in case of suspected Alzheimer's dementia (AD). Unfortunately, studies on optimal CSF biomarker cut-offs in a real-world clinical setting are scarce. Methods: We retrospectively evaluated the biomarker levels of 264 consecutive patients referred to our dementia clinic. The biomarkers were analysed with the Elecsys(R) assays. Diagnoses were based on all available clinical information, including FDG-PET scans. Results: In total, we identified 233 patients diagnosed with dementia. The median MMSE score was 22 (IQR 18–25). AD pathophysiology was suspected in 156 patients, and the corresponding cut-offs based on the Youden index were: Aβ42: 903 ng/L (ROC-AUC 0.78); t-tau: 272 ng/L (ROC-AUC 0.78); p-tau: 24 ng/L (ROC-AUC 0.85); t-tau/Aβ42 ratio: 0.34 (ROC-AUC 0.91); p-tau/Aβ42 ratio: 0.029 (ROC-AUC 0.92). Conclusions: We found the tau/Aβ42 ratios to possess the best diagnostic performance, but our estimated cut-off values for the ratios were somewhat higher than previously reported. Consequently, if the CSF analyses are used to support a diagnosis of AD in a heterogeneous high-prevalence cohort, adjustment of the cut-offs may be warranted.
Published: 2023
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5. Short-term biological variation of serum glial fibrillary acidic protein

Author: Silje Hovden Christensen, Claus Vinter Bødker Hviid, Anne Tranberg Madsen, Tina Parkner, and Anne Winther-Larsen
Subjects: biological variation, nervous system diseases, Brain Injuries, Traumatic/diagnosis, Biochemistry (medical), Clinical Biochemistry, biomarkers, General Medicine, depressive disorder, Reference Values, glial fibrillary acidic protein, Brain Injuries, Traumatic, Glial Fibrillary Acidic Protein, Linear Models, Humans, individual, Biomarkers
Abstract: Objectives Serum glial fibrillary acidic protein (GFAP) is an emerging biomarker for intracerebral diseases and is approved for clinical use in traumatic brain injury. GFAP is also being investigated for several other applications, where the GFAP changes are not always outstanding. It is thus essential for the interpretation of GFAP to distinguish clinical relevant changes from natural occurring biological variation. This study aimed at estimating the biological variation of serum GFAP. Methods Apparently healthy subjects (n=33) had blood sampled for three consecutive days. On the second day, blood was also drawn every third hour from 9 AM to 9 PM. Serum GFAP was measured by Single Molecule Array (Simoa™). Components of biological variation were estimated in a linear mixed-effects model. Results The overall median GFAP value was 92.5 pg/mL (range 34.4–260.3 pg/mL). The overall within– (CVI) and between-subject variations (CVG) were 9.7 and 39.5%. The reference change value was 36.9% for an increase. No day-to-day variation was observed, however semidiurnal variation was observed with increasing GFAP values between 9 AM and 12 PM (p Conclusions Serum GFAP exhibits a relatively low CVI but a considerable CVG and a marked semidiurnal variation. This implies caution on the timing of blood sampling and when interpreting GFAP in relation to reference intervals, especially in conditions where only small GFAP differences are observed.
Published: 2022
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6. Plasma calprotectin - preanalytical stability and interference from hemolysis

Author: Anne-Birgitte Garm Blavnsfeldt, Tina Parkner, and Cindy Soendersoe Knudsen
Subjects: Feces, Hemoglobins, Clinical Biochemistry, Humans, General Medicine, Hemolysis, Leukocyte L1 Antigen Complex, Biomarkers, Edetic Acid
Abstract: As an activation product of neutrophil granulocytes, calprotectin has been widely used in fecal samples for diagnosis and monitoring of patients with inflammatory bowel disease. However, fecal sample collection is cumbersome, and pre-analytical sources of error are plentiful. Therefore, plasma calprotectin is being investigated as a promising new biomarker. To utilize any biomarker, pre-analytical factors such as stability and susceptibility to interference from hemolysis must be established. We present precision estimates, stability results as well as interference study on plasma calprotectin in EDTA-plasma using the Thermo Fischer Phadia 250 EliA
Published: 2022
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7. Vitamin B12 deficiency: correction of P-methylmalonic acid for estimated glomerular filtration rate to improve diagnostic value - a confirmatory study

Author: Sebastian Nielsen, Tina Parkner, and Johan Frederik Håkonsen Arendt
Subjects: Vitamin B 12, methylmalonic acid, estimated glomerular filtration rate, Vitamin B12, kidney dysfunction, Clinical Biochemistry, Humans, Vitamin B 12 Deficiency, General Medicine, vitamin B12 deficiency, Biomarkers, Glomerular Filtration Rate, Methylmalonic Acid
Abstract: Objectives: For diagnosis of vitamin B12 deficiency, plasma methylmalonic acid (P-MMA) is considered superior to plasma vitamin B12 (P-B12). Reduced kidney function elevates P-MMA, hence, hampering P-MMA as a biomarker. We assessed whether correcting P-MMA for estimated glomerular filtration rate (eGFR) can affect the estimated prevalence of B12 deficiency. Methods: We included 115,245 patients with concomitant measurements of P-MMA, P-B12 and P-Creatinine. B12 deficiency was classified using P-MMA decision limits at >0.75 and >0.43 µmol/L. The non-linear relation between eGFR and P-MMA was estimated using spline regression. We calculated the percentage-wise reclassification of B12 deficiency by using an eGFR corrected P-MMA formula with eGFR reference points of 90 and 60 mL/min. Results: 6% with B12 deficiency were reclassified as non-deficient after adjusting for eGFR (reference point eGFR 90 mL/min) with both P-MMA decision limits. Overall B12 deficiency prevalence was reduced from 9.6% to 9.0% (P-MMA decision limit 0.43 µmol/L). With P-MMA decision limits at 0.75 and 0.43 µmol/L, 33.6% and 44.8% of B12 deficient patients with an eGFR
Published: 2022
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8. Biotin interference in routine IDS-iSYS immunoassays for aldosterone, renin, insulin-like growth factor 1, growth hormone and bone alkaline phosphatase

Author: Kasper Adelborg, Tina Parkner, Esben Søndergaard, and Cindy Soendersoe Knudsen
Subjects: Streptavidin, medicine.medical_specialty, Clinical chemistry, medicine.medical_treatment, Clinical Biochemistry, Biotin, DIAGNOSIS, Bone remodeling, chemistry.chemical_compound, Insulin-like growth factor, endocrinology, biotin, Internal medicine, Renin, Renin–angiotensin system, medicine, Humans, medical biochemistry, Insulin-Like Growth Factor I, Immunoassays, Aldosterone, Immunoassay, Growth factor, General Medicine, ADULTS, Alkaline Phosphatase, Endocrinology, HIGH-DOSE BIOTIN, chemistry, Growth Hormone, bone metabolism
Abstract: Biotin is increasingly used as dietary supplement. As many immunoassays rely on a binding between biotin and streptavidin, intake of biotin may interfere with laboratory tests, leading to spurious test results. We examined the extent to which levels of aldosterone, renin, insulin-like growth factor 1 (IGF-1), growth hormone (GH) and bone alkaline phosphatase (BAP) were affected by biotin. In an experimental study performed at Aarhus University Hospital, Denmark, patient samples (plasma or serum) were pooled and spiked with biotin in increasing concentrations (0, 20, 50, 100 and 500 ng/mL). All biomarkers were analyzed using Immunodiagnostic Systems (IDS-iSYS) Multi-Discipline Automated System assays. The average bias (%) was calculated, as the difference in concentrations between the sample without biotin (reference) and the samples with increasing concentrations of biotin. Both aldosterone and renin assays showed substantial biotin interference in a dose-dependent manner, with biases up to +3484% for aldosterone and −98% for renin in the highest concentrations of biotin (100–500 ng/mL). IGF-1, GH and BAP results were generally less affected by added biotin and significant bias (>10%) was observed only when the biotin concentration was 100 ng/mL (IGF-1 and GH) or 500 ng/mL (BAP). In conclusion, biotin interfered with the IDS-iSYS immunoassays, particularly for aldosterone and renin. The assays for GH, IGF-1 and BAP were less sensitive and only with high concentrations of biotin.
Published: 2022
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9. Reference interval and preanalytical properties of serum neurofilament light chain in Scandinavian adults

Author: Cindy Soendersoe Knudsen, Claus Vinter Bødker Hviid, and Tina Parkner
Subjects: Adult, Male, 0301 basic medicine, Oncology, blood chemical analysis, medicine.medical_specialty, Adolescent, Neurofilament light, Clinical Biochemistry, Neurofilament protein L, Scandinavian and Nordic Countries, 03 medical and health sciences, 0302 clinical medicine, Neurofilament Proteins, Reference Values, Internal medicine, medicine, Humans, Nerve Tissue, Aged, Aged, 80 and over, Blood Chemical Analysis, business.industry, Age Factors, General Medicine, Middle Aged, reference values, Healthy Volunteers, pre-analytical phase, 030104 developmental biology, Reference values, Biomarker (medicine), Female, business, serum, Biomarkers, 030217 neurology & neurosurgery
Abstract: The neurofilament light chain (NfL) is a promising biomarker of neuronal injury which is approaching routine clinical use. With the development of ultra-sensitive technologies, NfL has become measurable in the peripheral blood but the reference interval for serum NfL remains to be established. NfL was measured by a single-molecule array (Simoa™) analysis under internal and external quality control which is established for routine clinical use. Serum samples from 342 reference subjects, 18−87 years were analyzed. The age-partitioned reference interval was established according to Clinical and Laboratory Standards Institute guidelines, an approximation of the upper reference interval limit per 10-year age-groups was performed, and key pre-analytical properties were examined. Serum NfL levels increased 2.9% per year. The non-parametric reference interval for the age groups 18−40, 41−65, and >65 years were 2.8−9.7 ng/L, 4.6 − 21.4 ng/L, and 7.5−53.8 ng/L, respectively. The estimated upper reference interval limits per 10-year intervals corresponded well with the 90% confidence limits of the non-parametric reference interval. The recovery of serum NfL after seven days at room temperature or three freeze-thaw cycles were 93% (95% CI: 89%−97%) and 92% (95% CI: 83%−102%) and levels in serum were only slightly higher than levels in plasma (p
Published: 2020
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10. Procollagen III, N-terminal propeptide (PIIINP): Establishment of reference intervals in Northern European adults and children using the MAGLUMI 800 chemiluminescence immunoassay

Author: Julie Brogaard Larsen, Cindy Soendersoe Knudsen, and Tina Parkner
Subjects: Adult, Male, Adolescent, Chemiluminescence immunoassay, Liver fibrosis, Clinical Biochemistry, law.invention, Young Adult, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Reference Values, law, Humans, Child, Aged, Chemiluminescence, Immunoassay, Procollagen iii, Chemistry, Age Factors, General Medicine, N terminal propeptide, Middle Aged, Molecular biology, Peptide Fragments, Reference intervals, Method comparison, Child, Preschool, Luminescent Measurements, Biomarker (medicine), Female, 030211 gastroenterology & hepatology, Procollagen
Abstract: Procollagen III, N-terminal propeptide (PIIINP) is used as a biomarker for increased collagen III-synthesis. Reference intervals have not been established for the MAGLUMI 800 chemiluminescence immunoassay (CLIA) in Northern European adults or in children. The present study aimed to establish age-specific reference intervals in a Northern European population. PIIINP serum levels were analysed in healthy blood donors 19-67 years (
Published: 2021
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11. Anti-transglutaminase IgA antibody measurement in coeliac disease: Method comparison IDS-iSYS vs. Thermo Fisher Phadia

Author: Cindy Soendersoe Knudsen, Tina Parkner, and Birgitte Sandfeld-Paulsen
Subjects: Immunoglobulin A, Adult, Male, Tissue transglutaminase, Clinical Biochemistry, tissue transglutaminase, Coeliac disease, Serology, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Reference Values, medicine, antibodies, Humans, 030212 general & internal medicine, Aged, IgA antibody measurement, Transglutaminases, biology, business.industry, General Medicine, Middle Aged, medicine.disease, Immunodiagnostic Systems Ltd, Celiac Disease, Method comparison, Immunology, biology.protein, 030211 gastroenterology & hepatology, Female, Antibody, business
Abstract: In coeliac disease, the diagnostic work-up is based on a combination of clinical, histopathological and serological evaluation. Among the serological tests, the presence of tissue transglutaminase (tTG) IgA antibodies is the cornerstone owed to a high sensitivity and specificity. Recently, Immunodiagnostic Systems Ltd (IDS) introduced the fully automated chemiluminescent autoimmune assays for use on the IDS-iSYS Multi-Discipline Automated System. In this study, we aimed to compare the performance of the IDS-iSYS assay to the Thermo Fisher Phadia assay and to establish the upper reference limit of tTG IgA in a healthy population from Denmark based on the IDS-iSYS assay. We discovered a total imprecision of CV = 12.2% (2.87 AU/mL) and CV = 10.6% (47.55 AU/mL). Moreover, we compared the performance of IDS-iSYS assay to Thermo Fisher Phadia assay in 236 samples from unselected patients submit for tTG IgA testing and found a concordance of 97% (p
Published: 2020
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12. Plasma neurofilament light chain is associated with mortality after spontaneous intracerebral hemorrhage

Author: Niels Hjort, Claus Vinter Bødker Hviid, Tina Parkner, Anne-Mette Hvas, Signe Voigt Lauridsen, and Tua Gyldenholm
Subjects: Male, 0301 basic medicine, medicine.medical_specialty, Neurofilament light, Clinical Biochemistry, Gastroenterology, 03 medical and health sciences, 0302 clinical medicine, Neurofilament Proteins, Interquartile range, Internal medicine, Odds Ratio, Humans, Medicine, Prospective Studies, Spontaneous intracerebral hemorrhage, Aged, Cerebral Hemorrhage, cerebral hemorrhage, Receiver operating characteristic, business.industry, Biochemistry (medical), biomarkers, General Medicine, Odds ratio, Middle Aged, Prognosis, Survival Analysis, Confidence interval, neurofilament proteins, 030104 developmental biology, ROC Curve, Quartile, Area Under Curve, Biomarker (medicine), Female, prognosis, business, Biomarkers, 030217 neurology & neurosurgery
Abstract: Background Neurofilament light chain (NfL) is a neuron-specific biomarker with prognostic ability in several types of central nervous system injuries. This study investigates if plasma NfL (pNfL) is elevated early after spontaneous intracerebral hemorrhage (ICH) and whether such elevation reflects disease severity and day-30 outcome. Methods pNfL was quantified by single molecule array analysis in 103 reference subjects (RS) and in samples from 37 patients with ICH obtained on admission to hospital and at 24-h follow-up. The primary outcome was day-30 mortality. Clinical status on admission was evaluated by standardized scoring systems. Results Median pNfL among RS was 9.6 (interquartile range [IQR] 6.2) pg/mL. Upon admission, ICH patients had pNfL of 19.8 (IQR 30.7) pg/mL increasing to 35.9 (IQR 44.5) pg/mL at 24 h (all, p Conclusions PNfL is associated with day-30 mortality after spontaneous ICH when early after the ictus.
Published: 2020
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13. Plasma Neurofilament Light Chain Is Associated with Poor Functional Outcome and Mortality Rate After Spontaneous Subarachnoid Hemorrhage

Author: Claus Vinter Bødker Hviid, Tua Gyldenholm, Niels Sunde, Signe Voigt Lauridsen, Tina Parkner, and Anne-Mette Hvas
Subjects: 0301 basic medicine, BIOMARKER, Male, medicine.medical_specialty, Subarachnoid hemorrhage, Neurology, BLOOD, VASOSPASM, CSF, BRAIN-INJURY, Brain injuries, Gastroenterology, Neurofilament protein light, Severity of Illness Index, DELAYED CEREBRAL-ISCHEMIA, IMMUNOASSAY, Brain Ischemia, 03 medical and health sciences, 0302 clinical medicine, Cerebrospinal fluid, CEREBROSPINAL-FLUID, Modified Rankin Scale, Neurofilament Proteins, Internal medicine, medicine, Humans, Survival rate, business.industry, General Neuroscience, Mortality rate, Middle Aged, Subarachnoid Hemorrhage, medicine.disease, Confidence interval, 030104 developmental biology, Quartile, Female, Neurology (clinical), Cardiology and Cardiovascular Medicine, business, 030217 neurology & neurosurgery, Biomarkers
Abstract: The initial clinical status after subarachnoid hemorrhage (SAH) is an important outcome predictor, but the mechanisms behind the early brain injury (EBI) remains incompletely understood. Elevated neurofilament levels in the cerebrospinal fluid at protracted stages after SAH are associated with poor outcome, but the potential association between plasma neurofilament (pNfL) levels during EBI, disease severity on admission, and poor outcome remains unaddressed. Plasma NfL (pNfL) was measured by single molecule array in 44 SAH patients on admission and 24 h after ictus, as well as in 44 controls. Disease severity on admission was assessed by validated scoring systems, and day 30 modified Rankin Scale (mRS) score was registered. Admission levels of pNfL correlated with clinical disease severity scores (rho = 0.43, p 4) [odds ratio = 1.98, 95% confidence interval (CI): 1.01-3.88, p = 0.05]. Non-survivors had higher pNfL than survivors; on admission [17.6 pg/mL (IQR 11.4) vs. 8.4 pg/mL (IQR: 8.9), p
Published: 2019
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14. Diagnostic Accuracy of a Point-of-Care Test for Celiac Disease Antibody Screening among Infertile Patients

Author: Thomas Møller Jensen, Inge Agerholm, Bodil Hammer Bech, Louise Grode, Cecilia Høst Ramlau-Hansen, Tina Parkner, and Peter Humaidan
Subjects: medicine.medical_specialty, education.field_of_study, biology, business.industry, Point-of-care testing, Population, Gastroenterology, Gluten intolerance, Diagnostic accuracy, Disease, Serology, Positive predicative value, Internal medicine, biology.protein, Medicine, business, education, Gliadin, Research Article
Abstract: Background: Screening for celiac disease among infertile patients has been suggested. Several rapid point-of-care (POC) tests aimed at detecting celiac disease antibodies have been developed. It has been suggested that these POC tests can be implemented as a replacement for standard laboratory tests. Objective: To evaluatethe diagnostic accuracy of a POC test (Simtomax®) that detects celiac disease antibodies compared with standard laboratory tests when screening for celiac disease among patients referred for fertility treatment in 2 Danish fertility clinics. Methods: Serum samples were analyzed for IgA anti-tissue transglutaminase (TGA) as the reference standard test with a cutoff of ≥7 kU/L and by the index POC test based on IgA and IgG antibodies against deamidated gliadin peptides (DGP). In IgA deficiency, the reference standard test was IgG DGP with a cutoff of ≥7 kU/L. Participants answered a questionnaire on gluten intake, symptoms, and risk factors. Diagnostic confirmation was made by duodenal biopsies. IgA TGA/IgG DGP were used as the reference standard to calculate positive and negative predictive values. Results: A total of 622 men and women (51.6%) were enrolled during 2015. The reference standard IgA TGA/IgG DGP was positive in 7 participants (1.1% [95% CI 0.5–2.3]) and the POC test was positive in 84 participants (13.5% [95% CI 10.9–16.4]), 3 of whom also had positive reference standard tests. This yields a sensitivity of the index POC test of 42.9% (95% CI 9.9–81.6) and a specificity of 86.8% (95% CI 83.9–89.4). Positive and negative predictive values were 3.57% (95% CI 0.7–10.1) and 99.3% (95% CI 98.1–99.8). Conclusion: The sensitivity of the POC test was low; however, the specificity was moderately good. The POC test had a high negative predictive value in this low prevalent population but missed 1 patient with biopsy-confirmed celiac disease. However, because of many false-positive tests, it cannot be recommended as replacement for standard laboratory tests but rather as a triage test to decide if the standard serology tests should be performed.
Published: 2019
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15. Reference limits for GAD65 and IA-2 autoantibodies by chemiluminescence immunoassay in Northern European adults and children

Author: Cindy Soendersoe Knudsen, Agnes Ziobrowska-Bech, Britta Kremke, Anne Winther-Larsen, and Tina Parkner
Subjects: Male, Luminescence, endocrine system diseases, Chemiluminescence immunoassay, Clinical Biochemistry, Gene Expression, 01 natural sciences, 0302 clinical medicine, immune system diseases, Reference Values, Healthy volunteers, antibodies, 030212 general & internal medicine, Child, Immunoassay, biology, Glutamate Decarboxylase, General Medicine, Middle Aged, Healthy Volunteers, Europe, Child, Preschool, diabetes mellitus, Female, Antibody, Adult, Adolescent, Immunoassay/standards, European Continental Ancestry Group, White People, Glutamate Decarboxylase/antagonists & inhibitors, paediatrics, 03 medical and health sciences, Diabetes mellitus, medicine, Humans, Receptor-Like Protein Tyrosine Phosphatases, Class 8, Aged, Autoantibodies, Type 1 diabetes, Receptor-Like Protein Tyrosine Phosphatases, Class 8/antagonists & inhibitors, ICA512 autoantibody, business.industry, 010401 analytical chemistry, Autoantibody, Infant, Newborn, nutritional and metabolic diseases, Infant, Diabetes Mellitus, Type 1/blood, medicine.disease, 0104 chemical sciences, Diabetes Mellitus, Type 1, Reference values, GAD65, Immunology, biology.protein, business, Autoantibodies/blood, Biomarkers/blood, Biomarkers
Abstract: The GAD65 and IA-2 antibodies (Abs) are biomarkers of the development of type 1 diabetes mellitus (T1DM) in both children and adults. The upper reference limit for the autoantibodies made by the manufacture was established on an adult Chinese population. Here, we established upper reference limits for Northern European adults and children in accordance with the Clinical and Laboratory Standards Institute (CLSI) guidelines. Serum samples from healthy Danish children (0–18 years) and adults (18–70 years) were analysed for GAD65Ab and IA-2Ab using MAGLUMI 800 Chemiluminescence Immunoassay (CLIA). The Kruskal–Wallis test was used for evaluating differences between gender and age groups. No gender or age differences were found for neither GAD65Ab nor IA-2Ab, and a combined upper reference limit for both children and adults could be established. An upper reference limit of 5.1 IU/mL was defined for GAD65Ab and 11.5 U/mL for IA-2Ab. Our results showed a substantial discrepancy with the reference limits established by the manufacturer.
Published: 2019
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16. Home Management of Warfarin Treatment Through a Real-Time Supervised Telemedicine Solution:A Randomized Controlled Trial

Author: Ivan Brandslund, Tina Parkner, Jonna Skov Madsen, and Claus Lohman Brasen
Subjects: Male, INR, medicine.medical_specialty, Telemedicine, 020205 medical informatics, Point-of-Care Systems, Point-of-care testing, MEDLINE, Health Informatics, 02 engineering and technology, Vitamin k, POCT, law.invention, home management, Patient Education as Topic, Health Information Management, Randomized controlled trial, law, 0202 electrical engineering, electronic engineering, information engineering, medicine, Coaguchek XS, Humans, International Normalized Ratio, Aged, business.industry, fungi, Warfarin, Warfarin treatment, Anticoagulants, General Medicine, Middle Aged, Self Care, warfarin, Home management, Emergency medicine, randomized controlled trial, Female, telemedicine, Drug Monitoring, business, medicine.drug
Abstract: BACKGROUND: Many patients are undergoing oral anticoagulation treatment with vitamin K antagonists, which necessitates measuring international normalized ratio (INR) several times each month.INTRODUCTION: Patients can learn to measure their INR at home and choose their own dose for the next period with potential gains in treatment quality and reduced healthcare expenses. This is, however, connected to the potential problem of losing tight external control of the patient treatment.MATERIALS AND METHODS: We performed a randomized controlled trial using the telemedicine software CSO/AC together with the INR point-of-care-test CoaguChek XS for 10 months to investigate the use of criteria-driven healthcare interactions. A total of 87 patients were divided into two groups. The patient self-management (PSM) group was surveilled using the criteria INR 4.5, change in warfarin/week >1.25 mg, missing INR or dosage. The patient self-testing (PST) group was handled as routine care.RESULTS: A total of 84 patients were followed for 10 months. No differences were seen in average INR or fraction of INR in therapeutic range (2-3) in the two groups or the start compared with the end. The PST group was handled using 4.2 interactions per month whereas the PSM group used 1.1 interactions per month. No adverse effects of PSM were observed.DISCUSSION: Using criteria-driven interactions enabled a considerable reduction in interactions per month. The two groups were comparable in terms of treatment effect and safety.CONCLUSIONS: Using criteria to guide PSM interactions maintains good treatment effect while reducing healthcare expenses.
Published: 2019
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17. Unrecognised coeliac disease among men and women undergoing fertility treatment:A screening study

Author: Louise Grode, Bodil Hammer Bech, Inge Agerholm, Cecilia Høst Ramlau-Hansen, Peter Humaidan, Thomas Møller Jensen, and Tina Parkner
Subjects: Infertility, medicine.medical_specialty, Pediatrics, media_common.quotation_subject, prevalence, Fertility, macromolecular substances, Disease, Coeliac disease, reproduction, 03 medical and health sciences, 0302 clinical medicine, Epidemiology, Medicine, media_common, chemistry.chemical_classification, Autoimmune disease, 030219 obstetrics & reproductive medicine, business.industry, screening, Gastroenterology, nutritional and metabolic diseases, Original Articles, medicine.disease, Gluten, digestive system diseases, Oncology, chemistry, 030211 gastroenterology & hepatology, epidemiology, Reproduction, business, infertility, coeliac disease
Abstract: Background: Coeliac disease is an autoimmune disease triggered by dietary gluten and has been associated with several conditions influencing female and male reproduction. Due to unspecific symptoms, coeliac disease can be unrecognised for years.Objective: To estimate the prevalence of unrecognised coeliac disease among couples referred to fertility treatment.Methods: Cross-sectional screening for coeliac disease in men and women referred to fertility treatment using IgA tissue transglutaminase antibodies as a marker of coeliac disease and small-bowel biopsies to confirm the diagnosis. Participants answered a questionnaire on gluten intake, gastrointestinal symptoms and reproductive history.Results: A total of 893 participants (51% women) were screened and eight were coeliac disease antibody positive. Small-bowel biopsies were obtained from seven antibody positive participants and unrecognised coeliac disease was confirmed in one woman and three men, corresponding to a prevalence of 0.45% (95% confidence interval 0.12-1.14). The total prevalence, combining already diagnosed and unrecognised CD cases, was 0.63% (95% confidence interval 0.29-1.12).Conclusion: The prevalence of unrecognised coeliac disease in a group of infertile patients was equivalent to that of the Danish general population and low compared with that observed in the majority of other screening studies of infertile patients. Surprisingly, it should be noted that more men than women had coeliac disease. This result does not support a need for routine screening among infertile patients.
Published: 2018
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18. A Comparison of ree Chromogranin A Assays in Patients with Neuroendocrine Tumours

Author: Lise Brehm Hoej, Tina Parkner, Cindy Soendersoe Knudsen, and Henning Grønbæk
Subjects: Adult, Male, endocrine system, medicine.medical_specialty, Pathology, Radioimmunoassay, Renal function, Enzyme-Linked Immunosorbent Assay, Single-photon emission computed tomography, Gastroenterology, Predictive Value of Tests, Internal medicine, Biomarkers, Tumor, medicine, Humans, In patient, Aged, Aged, 80 and over, biology, medicine.diagnostic_test, business.industry, Reproducibility of Results, Chromogranin A, Middle Aged, Prognosis, Neuroendocrine Tumors, Positron emission tomography, Predictive value of tests, Clinical diagnosis, biology.protein, Female, business
Abstract: Background & Aims: Chromogranin A (CgA) is the most important general tumour marker used in the diagnosis and follow-up of patients with neuroendocrine tumours (NET). Chromogranin A assays may have different sensitivities, which is of importance for the clinical diagnosis and handling of NET patients. The aim of this study was to compare the clinical sensitivities of three different CgA assays in NET patients.Methods: We measured CgA level in 42 NET patients (male/female: 23/19, median age: 63 years, range 29- 85 years). Twenty-five patients had liver metastases, eight had local disease, and nine were disease free after surgery. We studied an in-house RIA: RH RIA assay (Rigshospitalet, Copenhagen, Denmark); NEOLISATM (Euro Diagnostica, Malmö, Sweden) and EURIA CgA RIA (Euro Diagnostica, Malmö, Sweden).Results: The RH RIA assay showed a clinical sensitivity of 97%, while the NEOLISA and EURIA assays both showed similar clinical sensitivities of 79%. Patients with liver metastases had significantly higher CgA levels compared to disease free patients by all three assays (P
Published: 2014
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19. Circadian variation in the pharmacokinetics of steady state continuous subcutaneous infusion of growth hormone in adult growth hormone deficient patients

Author: T. Lauritzen, Torben Laursen, Tina Parkner, Jurgita Janukonyte, and Jørgen S. Christiansen
Subjects: Adult, Male, medicine.medical_specialty, CLEARANCE, Evening, Supine position, DAILY INJECTIONS, Endocrinology, Diabetes and Metabolism, Infusions, Subcutaneous, Endocrinology, Pharmacokinetics, Internal medicine, Humans, Medicine, Distribution (pharmacology), Tissue Distribution, Circadian rhythm, Growth Disorders, INDEX, Aged, Morning, Immunoassay, Cross-Over Studies, GH BINDING-PROTEIN, Human Growth Hormone, HALF-LIFE, business.industry, TREATMENT PROFILE, Diurnal temperature variation, MEN, Middle Aged, Prognosis, WELL, Circadian Rhythm, Growth hormone deficient, Bioavailability, Diurnal variation, Continuous subcutaneous infusion, Area Under Curve, OBESITY, Exogenous growth hormone, BIOELECTRICAL-IMPEDANCE, Female, business, Follow-Up Studies
Abstract: Background: Previous studies in growth hormone (GH)-deficient (GHD) patients have indicated a possible diurnal variation in the pharmacokinetics (PK) of GH after subcutaneous (Sc) GH administration. Thus, higher GH levels were observed during the night with continuous sc infusion, and increased GH bioavailability was reported following daily sc injections in the evening compared to morning.Objective: The aim was to study whether diurnal variability in the PK of sc administered exogenous GH can be reproduced under standard conditions for all study participants, e.g. supine rest.Design and methods: Eight male GHD patients (59.8 +/- 8 years, body mass index 29.7 +/- 4.9 kg/m(2)) received a continuous sc infusion of GH (3 mg/24 h) for 60 h on two different occasions. Diurnal variation in PK of GH was studied during steady state in the last 24 h of the infusion period.Results: Median GH levels were higher at night time (23:00 h-07:00 h) than during the day (10:00 h-18:00 h) for visit 115.1 (4.5-72 ng/ml/0.5 h) vs. 4.6 (3.7-5.7 ng/ml/0.5 h); p Conclusions: Previous findings of higher nocturnal GH levels were confirmed during steady state continuous sc GH infusion under standard conditions. The underlying mechanisms, e.g. whether GH absorption, distribution or elimination is primarily affected need to be further elucidated. (C) 2013 Elsevier Ltd. All rights reserved.
Published: 2013
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20. Soluble CD163: a biomarker linking macrophages and insulin resistance

Author: Bente Klarlund Pedersen, Lars Peter Sørensen, Bo Martin Bibby, Christian P. Fischer, Holger Jon Møller, Anders R. Nielsen, Tina Parkner, and Søren Nielsen
Subjects: Adult, Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Antigens, Differentiation, Myelomonocytic, Receptors, Cell Surface, Type 2 diabetes, Biology, Impaired glucose tolerance, Insulin resistance, Antigens, CD, Internal medicine, Diabetes mellitus, Glucose Intolerance, Internal Medicine, medicine, Humans, Aged, Univariate analysis, Glucose tolerance test, medicine.diagnostic_test, Interleukin-6, Tumor Necrosis Factor-alpha, Macrophages, Insulin, nutritional and metabolic diseases, Glucose Tolerance Test, Middle Aged, medicine.disease, Cross-Sectional Studies, Endocrinology, Diabetes Mellitus, Type 2, Case-Control Studies, Female, Insulin Resistance, Metabolic syndrome
Abstract: Soluble CD163 (sCD163) was recently identified as a strong risk marker for developing type 2 diabetes. We hypothesised that sCD163 independently associates with insulin resistance. This cross-sectional study includes 234 participants: 96 with type 2 diabetes, 34 with impaired glucose tolerance (IGT) and 104 with normal glucose tolerance (NGT), matched for sex and BMI. Glucose-lowering medication was paused for 1 week before plasma samples were obtained for determination of sCD163 and other inflammatory and metabolic variables. Insulin resistance was estimated by homeostasis model assessment of insulin resistance (HOMA-IR). Concentrations of sCD163 were 1.95 mg/l (0.63–6.97) in individuals with type 2 diabetes, 1.64 mg/l (0.58–4.19) in those with IGT, and 1.48 mg/l (0.48–4.11) (median [range]) in those with NGT (p
Published: 2012
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21. Insulin and glucose profiles during continuous subcutaneous insulin infusion compared with injection of a long-acting insulin in Type 2 diabetes1

Author: Torben Laursen, Esben Thyssen Vestergaard, Jørgen S. Christiansen, T. Lauritzen, J. S. Smedegaard, Tina Parkner, and H. Hartvig
Subjects: Insulin pump, medicine.medical_specialty, business.industry, Insulin glargine, Endocrinology, Diabetes and Metabolism, Insulin, medicine.medical_treatment, Type 2 diabetes, medicine.disease, Insulin aspart, Subcutaneous injection, Endocrinology, Basal (medicine), Internal medicine, Diabetes mellitus, Internal Medicine, medicine, business, medicine.drug
Abstract: Aims To compare insulin and glucose profiles during basal continuous subcutaneous infusion of a rapid-acting insulin analogue and once daily subcutaneous injection of a long-acting insulin analogue in Type 2 diabetes. Methods Twenty-one patients with Type 2 diabetes treated with oral glucose-lowering agents were randomized in this two-period crossover study to an equivalent 24-h dose of continuous subcutaneous infusion of insulin aspart and subsequently once-daily bedtime subcutaneous injection of insulin glargine, or vice versa, for eight consecutive days. Plasma profiles of insulin and glucose were recorded. Results On the last day of each treatment period, the area under the curve (AUC) for glucose was 10% lower on the continuous subcutaneous infusion regimen compared with the insulin injection regimen (P = 0.002). This was accomplished by a flat exogenous insulin infusion profile compared with a peaking profile with injected insulin (AUC was 74% higher after injection compared with pre-injection levels (P = 0.001)). During the last 6 days in each treatment period, the intra-subject variability of exogenous fasting insulin levels in the mornings was 41% lower during insulin infusion compared with insulin injection (P = 0.012). The corresponding intra-subject variability for fasting glucose only showed a tendency to be lower during infusion as compared to the injection regimen (28%; P = 0.104). Thirteen symptomatic-only or minor hypoglycaemic episodes were recorded during the entire infusion period compared with three episodes during the injection period. Conclusions Basal continuous subcutaneous infusion of a rapid-acting insulin analogue improved plasma insulin (more flat insulin profile with a lower variability) and glucose (lower AUC) profiles compared with once-daily subcutaneous injection of a long-acting insulin analogue in Type 2 diabetes.
Published: 2008
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22. Interaction between exogenous insulin, endogenous insulin, and glucose in type 2 diabetes patients

Author: Niels Henrik Bruun, Torsten Lauritzen, Torben Laursen, Jens Sandahl Christiansen, Jurgita Janukonyte, and Tina Parkner
Subjects: Blood Glucose, Male, medicine.medical_specialty, endocrine system diseases, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Type 2 diabetes, Infusions, Subcutaneous, Drug Administration Schedule, Insulin aspart, Endocrinology, Diabetes mellitus, Internal medicine, Medicine, Humans, Hypoglycemic Agents, Insulin, Insulin Aspart, Aged, Cross-Over Studies, business.industry, nutritional and metabolic diseases, Type 2 Diabetes Mellitus, Middle Aged, medicine.disease, Crossover study, Metformin, Medical Laboratory Technology, Diabetes Mellitus, Type 2, Hyperglycemia, Female, business, Body mass index, medicine.drug
Abstract: BACKGROUND: Little is known about the influence of exogenous insulin and actual glucose levels on the release of endogenous insulin in insulin-treated type 2 diabetes mellitus (T2DM) patients. This study investigated the interaction among serum endogenous insulin (s-EI), serum exogenous insulin aspart (s-IAsp), and blood glucose levels in an experimental short-term crossover design.STUDY DESIGN AND METHODS: Eight T2DM patients (63.52 years old; range, 49-69 years; mean body mass index, 28.8±3.8 kg/m2) were randomized to treatment with individual fixed doses of insulin aspart (0.5-1.5 IU/h) as a continuous subcutaneous insulin infusion (CSII) during a 10-h period on two occasions with different duration of hyperglycemia: (1) transient hyperglycemia for 2 h (visit TH) and (2) continuous hyperglycemia for 12 h (visit CH).RESULTS: During steady state the variances of plasma glucose (p-glucose), s-IAsp, and s-EI were equal within visit TH and within visit CH, but variances were significantly higher during visit CH compared with visit TH. The s-IAsp reached lower levels at visit CH compared with visit TH (test for slope=1, P=0.005). The s-EI depended on p-glucose in a nonlinear fashion during the first 100 min of both visits when s-IAsp was undetectable (adjusted R2=0.9). A complex but statistically significant interaction among s-IAsp, s-EI, p-glucose, and patients was observed during measurable s-IAsp levels (adjusted R2=0.70).CONCLUSIONS: Endogenous and exogenous insulin showed higher variation during continuous hyperglycemia. Significantly lower levels of exogenous insulin were observed following CSII during continuous hyperglycemia compared with transient hyperglycemia. Endogenous insulin levels could in a complex way be explained by an individual interaction among p-glucose and serum exogenous insulin, if present. BACKGROUND: Little is known about the influence of exogenous insulin and actual glucose levels on the release of endogenous insulin in insulin-treated type 2 diabetes mellitus (T2DM) patients. This study investigated the interaction among serum endogenous insulin (s-EI), serum exogenous insulin aspart (s-IAsp), and blood glucose levels in an experimental short-term crossover design.STUDY DESIGN AND METHODS: Eight T2DM patients (63.52 years old; range, 49-69 years; mean body mass index, 28.8±3.8 kg/m(2)) were randomized to treatment with individual fixed doses of insulin aspart (0.5-1.5 IU/h) as a continuous subcutaneous insulin infusion (CSII) during a 10-h period on two occasions with different duration of hyperglycemia: (1) transient hyperglycemia for 2 h (visit TH) and (2) continuous hyperglycemia for 12 h (visit CH).RESULTS: During steady state the variances of plasma glucose (p-glucose), s-IAsp, and s-EI were equal within visit TH and within visit CH, but variances were significantly higher during visit CH compared with visit TH. The s-IAsp reached lower levels at visit CH compared with visit TH (test for slope=1, P=0.005). The s-EI depended on p-glucose in a nonlinear fashion during the first 100 min of both visits when s-IAsp was undetectable (adjusted R(2)=0.9). A complex but statistically significant interaction among s-IAsp, s-EI, p-glucose, and patients was observed during measurable s-IAsp levels (adjusted R(2)=0.70).CONCLUSIONS: Endogenous and exogenous insulin showed higher variation during continuous hyperglycemia. Significantly lower levels of exogenous insulin were observed following CSII during continuous hyperglycemia compared with transient hyperglycemia. Endogenous insulin levels could in a complex way be explained by an individual interaction among p-glucose and serum exogenous insulin, if present.
Published: 2015
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23. Visceral obesity is associated with increased soluble CD163 concentration in men with type 2 diabetes mellitus

Author: Bo Martin Bibby, Holger Jon Møller, Esben Søndergaard, Søren Nielsen, Lars Peter Sørensen, and Tina Parkner
Subjects: obesity, medicine.medical_specialty, Multivariate analysis, type 2 diabetes mellitus, Endocrinology, Diabetes and Metabolism, Adipose tissue, White adipose tissue, Overweight, Endocrinology, Insulin resistance, white adipose tissue, Internal medicine, Internal Medicine, medicine, Glycemic, soluble CD163, business.industry, Research, Type 2 Diabetes Mellitus, nutritional and metabolic diseases, medicine.disease, Obesity, macrophages, inflammation, medicine.symptom, business
Abstract: Monocyte/macrophage-specific soluble CD163 (sCD163) concentration is associated with insulin resistance and increases with deteriorating glycemic control independently of BMI. This led to the proposal of the hypothesis that obesity-associated white adipose tissue inflammation varies between individuals. The objective was to examine the effect of male overweight/obesity and type 2 diabetes mellitus (T2DM) on associations between adiposity parameters and sCD163. A total of 23 overweight/obese non-diabetic men, 16 overweight/obese men with T2DM, and a control group of 20 normal-weight healthy men were included. Body composition and regional body fat distribution were determined by whole-body dual X-ray absorptiometry scan and abdominal computed tomography (CT) scan. Serum sCD163 concentrations were determined by ELISA. Associations between adiposity parameters and sCD163 were investigated using multiple linear regression analysis. In the normal-weight healthy men, there was no significant association between adiposity parameters and sCD163, whereas in the overweight/obese non-diabetic men, measures of general and regional adiposity were positively associated with sCD163. In the overweight/obese men with T2DM, only visceral adipose tissue (VAT) and the ratio of VAT to abdominal subcutaneous adipose tissue (SAT), a measure of relative body fat distribution between VAT and SAT depots, were positively associated with sCD163. In a multivariate analysis, including VAT, upper-body SAT, and lower-body fat, adjusted for BMI and age, VAT remained a significant predictor of sCD163 in the overweight/obese T2DM men, but not in the overweight/obese non-diabetic men. Our results indicate that VAT inflammation is exaggerated in men with T2DM, and that propensity to store excess body fat viscerally is particularly detrimental in men with T2DM.
Published: 2015
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24. Effects of human insulin and insulin aspart preparations on levels of IGF-I, IGFBPs and IGF bioactivity in patients with type 1 diabetes

Author: Zhulin Ma, Jan Frystyk, Torben Laursen, Torsten Lauritzen, Tina Parkner, Chunsen Wu, and Jens Sandahl Christiansen
Subjects: medicine.medical_specialty, medicine.medical_treatment, Endocrinology, Diabetes and Metabolism, BINDING PROTEIN-1 IGFBP-1, Insulin aspart, MELLITUS, HORMONE, Internal medicine, Diabetes mellitus, GLYCEMIC CONTROL, GROWTH-FACTOR (IGF)-I, medicine, Insulin, IGFBP, TOLERANCE, IGF, Receptor, Insulin analogue, HUMAN-SERUM, Type 1 diabetes, FACTOR SYSTEM, business.industry, Kinase, FACTOR AXIS, Area under the curve, General Medicine, medicine.disease, Crossover study, Endocrinology, GLUCOSE-HOMEOSTASIS, business, medicine.drug, Research Article
Abstract: BACKGROUND: Insulin aspart (IAsp) and its biphasic preparations BIAsp50 and BIAsp70 (containing 50% and 70% IAsp, respectively) have distinct glucose-lowering properties as compared to human insulin (HI). We investigated whether this affected the circulating IGF-system which depends on the hepatic insulin exposure.METHODS: In a randomized, four-period crossover study, 19 patients with type 1 diabetes received identical doses (0.2 U/kg sc) of IAsp, BIAsp70, BIAsp50 and HI together with a standardized meal. Serum total IGF-I and IGFBP-1 to -3 were measured by immunoassays for nine hours post-prandially. Bioactive IGF was determined by an in-house, cell-based IGF-I receptor kinase activation (KIRA) assay.RESULTS: Despite marked differences in peripheral insulin concentrations and plasma glucose, the four insulin preparations resulted in parallel decreases in IGFBP-1 levels during the first 3 hours, and parallel increases during the last part of the study (3-9 hours). Thus, only minor significances were seen. Insulin aspart and human insulin resulted in a lower area under the curve (AUC) during the first 3 hours as compared to BIAsp70 (p = 0.009), and overall, human insulin resulted in a lower IGFBP-1 AUC than BIAsp70 (p = 0.025). Nevertheless, responses and AUCs of bioactive IGF were similar for all four insulin preparations. Changes in levels of bioactive IGF were inversely correlated to those of IGFBP-1, increasing during the first 3 hours, whereafter levels declined (-0.83 ≤ r ≤ -0.30; all p-values CONCLUSIONS: Despite distinct glucose-lowering properties, the tested insulin preparations had similar effects on IGF-I concentration and IGF bioactivity, IGFBP-2 and IGFBP-3 as compared to HI; only small differences in IGFBP-1 were seen and they did not affect bioactive IGF. Thus, insulin aspart containing preparation behaves as HI in regards to the circulating IGF-system. However, bioactive IGF appeared to be more sensitive to insulin exposure than total IGF-I. The physiological significance of this finding remains to be determined.TRIAL REGISTRATION: NCT00888732.
Published: 2014
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25. Impact of moderate interval exercise versus supine rest on the pharmacokinetics and pharmacodynamic profiles of subcutaneously administered growth hormone in adult growth hormone deficient patients

Author: J. Janukonytė, Jørgen S. Christiansen, Jan Frystyk, H.S. Pedersen, Torben Laursen, T. Lauritzen, and Tina Parkner
Subjects: Adult, Blood Glucose, Male, medicine.medical_specialty, Supine position, Injections, Subcutaneous, Rest, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, INJECTIONS, Hypopituitarism, Endocrinology, Pharmacokinetics, Internal medicine, Supine Position, medicine, ABSORPTION, Humans, Insulin, Dosing, Exercise, Rest (music), Aged, Human Growth Hormone, business.industry, INFUSION, Blood flow, Middle Aged, Growth hormone secretion, Circadian Rhythm, Diurnal variation, Pharmacodynamics, Exogenous growth hormone, GH DEFICIENCY, business
Abstract: Background: Diurnal variation in serum growth hormone (s-GH) levels after exogenous GH delivery has previously been repotted in patients with no endogenous GH secretion. Changes in postural position or physical activity, leading to changes in blood flow and/or lymphatic drainage may be underlying explanations.Primary objectives: The primary aim of this study is to study a possible impact of exercise and supine rest on pharmacokinetics (PK) and day-to-day variation of subcutaneously (sc) administered GH in adult GH deficient (AGHD) patients.Secondary objective: The secondary aim of this study is to compare s-IGF-I, s-insulin, and plasma (p)-glucose profiles after a carbohydrate rich breakfast following sc GM injection vs. continuous infusion.Design and methods: During supine rest eight AGHD males (59.8 +/- 8 years, BMI 29.7 +/- 4.9 kg/m(2)) were treated with one daily sc GM injections of 3 mg/24 h for 48 h (treatment sessions A, B) or a continuous sc GM infusion of 3 mg/24 h for 60 h (treatment sessions C, D). Exercise comprised 1 h bicycling with 50 W load on two consecutive days during treatment sessions B and D.Results: Administration of GM as a bolus injection, but not as a continuous GH infusion, resulted in about 32% higher s-GM levels during exercise (60 min) as well as 30 min after (s-GH logAUC((B-A)) difference was 0.28; 95% CI: 0.14-.0.4; p Conclusions: Moderate exercise intermittently increased s-GH levels. These changes seem to have no clinical short-term relevance, since total s-GH(24 h) and s-IGF-I-48 h levels were unaffected. (C) 2014 Elsevier Ltd. All rights reserved.
Published: 2014
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26. Measurement of Rhodopsin in Serum from 40 Patients with Diabetic Retinopathy

Author: Ivan Brandslund, Eva Rabing Brix Petersen, Tina Parkner, Cramer Kjeldahl Christensen, and Søren Bernth Hansen
Subjects: genetic structures, sense organs
Abstract: Measurement of Rhodopsin in Serum from 40 Patients with Diabetic Retinopathy.
Published: 2014

27. A comparison of three chromogranin A assays in patients with neuroendocrine tumours

Author: Lise Brehm Høj, Tina Parkner, Cindy Soendersoe Knudsen, and Henning Grønbæk
Subjects: endocrine system
Abstract: BACKGROUND AND AIMS: Chromogranin A (CgA) is the most important general tumour marker used in the diagnosis and follow-up of patients with neuroendocrine tumours (NET). Chromogranin A assays may have different sensitivities, which is of importance for the clinical diagnosis and handling of NET patients. The aim of this study was to compare the clinical sensitivities of three different CgA assays in NET patients.METHODS: We measured CgA level in 42 NET patients (male/female: 23/19, median age: 63 years, range 29-85 years). Twenty-five patients had liver metastases, eight had local disease, and nine were disease free after surgery. We studied an in-house RIA: RH RIA assay (Rigshospitalet, Copenhagen, Denmark); NEOLISATM (Euro Diagnostica, Malmö, Sweden) and EURIA CgA RIA (Euro Diagnostica, Malmö, Sweden).RESULTS: The RH RIA assay showed a clinical sensitivity of 97%, while the NEOLISA and EURIA assays both showed similar clinical sensitivities of 79%. Patients with liver metastases had significantly higher CgA levels compared to disease free patients by all three assays (PCONCLUSION: Chromogranin A measurements are significantly assay-dependent and caution should be applied in the interpretation of CgA measurement for assessment of NET status. The in-house RH RIA assay was better at predicting NET status than the NEOLISA and EURIA assays.
Published: 2014

28. Effects of human insulin and insulin aspart preparations on levels of IGFI, IGFBPs and IGFI bioactivity in patients with type 1 diabetes

Author: Torben Laursen, Jens Sandahl Christiansen, Zhulin Ma, Torsten Lauritzen, Jan Frystyk, and Tina Parkner
Subjects: Insulin aspart, Type 1 diabetes, medicine.medical_specialty, Endocrinology, business.industry, Internal medicine, medicine, Human insulin, In patient, medicine.disease, business, medicine.drug
Published: 2013
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29. A comparison of pharmacokinetics and pharmacodynamics of insulin aspart, biphasic insulin aspart 70, biphasic insulin aspart 50, and human insulin: a randomized, quadruple crossover study

Author: Torben Laursen, Torsten Lauritzen, Jan Frystyk, Zhulin Ma, Jens Sandahl Christiansen, and Tina Parkner
Subjects: Adult, Blood Glucose, Male, medicine.medical_specialty, Time Factors, endocrine system diseases, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Injections, Subcutaneous, Biphasic Insulins, Body Mass Index, Insulin aspart, Young Adult, Endocrinology, Pharmacokinetics, Internal medicine, Diabetes mellitus, medicine, Humans, Hypoglycemic Agents, Insulin, Insulin Aspart, Type 1 diabetes, Cross-Over Studies, business.industry, nutritional and metabolic diseases, Middle Aged, medicine.disease, Crossover study, Medical Laboratory Technology, Diabetes Mellitus, Type 1, Pharmacodynamics, Area Under Curve, Female, business, hormones, hormone substitutes, and hormone antagonists, medicine.drug
Abstract: We compared the pharmacokinetic and pharmacodynamic profiles of insulin aspart, biphasic insulin aspart 70 (BIAsp70) and 50 (BIAsp50) (containing 70% and 50% rapid-acting insulin aspart, respectively), and soluble human insulin under experimental conditions.In this randomized, four-period crossover study, 19 type 1 diabetes patients received subcutaneous injections of identical doses (0.2 U/kg) of insulin aspart, BIAsp70, or BIAsp50 immediately before a standardized meal or human insulin 30 min before meal. Plasma glucose and serum insulin were measured for 12 h postprandially.The pharmacokinetic and pharmacodynamic profiles of human insulin differed from those of insulin aspart, BIAsp70, and BIAsp50. The three different aspart preparations had easily distinguishable features with regard to onset and duration of action. Insulin aspart preparations were, on average, absorbed twice as fast as human insulin. In the initial phases (0-4 h and 0-6 h), the insulin area under the concentration-time curve (AUC(ins)) was significantly higher during insulin aspart treatment compared with the others, whereas insulin aspart had a significantly lower AUC(ins) over the last 6 h (P0.05). BIAsp70 and BIAsp50 provided insulin coverage comparable to that of human insulin over the last 6 h. Insulin aspart had the most pronounced onset of action and the shortest duration. Comparing with insulin aspart and BIAsp70, BIAsp50 revealed a closer treatment ratio to human insulin on pharmacodynamic end points.BIAsp70 and BIAsp50 injected immediately before a meal are at least as effective as human insulin injected 30 min earlier in controlling postprandial glycemic excursions. BIAsp50 showed the greatest similarity to human insulin with regard to pharmacokinetic and pharmacodynamic profiles.
Published: 2012
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30. IDegAsp:a novel soluble insulin analogs combination

Author: Zhulin Ma, Torben Laursen, Jens Sandahl Christiansen, and Tina Parkner
Subjects: Blood Glucose, Insulin degludec, medicine.medical_treatment, Molecular Sequence Data, Clinical Biochemistry, Insulin analog, Type 2 diabetes, Pharmacology, Hypoglycemia, Insulin aspart, Diabetes mellitus, Drug Discovery, medicine, Animals, Humans, Hypoglycemic Agents, Insulin, Amino Acid Sequence, Glycemic, business.industry, medicine.disease, Insulin, Long-Acting, Diabetes Mellitus, Type 2, business, medicine.drug
Abstract: INTRODUCTION: Current rapid- and long-acting insulin analogs cannot maintain their individual pharmacokinetic profile when they are co-formulated. IDegAsp , a novel soluble combination was developed with rapid-acting insulin aspart and a new-generation ultra-long-acting insulin, insulin degludec and was anticipated to offer clinical advantage over available premixed insulin suspensions. AREAS COVERED: We reviewed published data regarding pharmacological characters, clinical efficacy, safety, and tolerability of IDegAsp. Literature was searched through the electronic medical databases (PubMed, EMBASE, and Web of Knowledge) up to June 2012. EXPERT OPINION: Preliminary clinical data indicate that IDegAsp is a safe, well-tolerated insulin combination and provides a similar overall glycemic control to current insulin preparations with a reduced risk of hypoglycemia. IDegAsp might be a promising treatment option for patients with type 2 diabetes who need to improve control of postprandial glucose excursions and fasting glucose levels.
Published: 2012
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31. Do all patients with type 2 diabetes need breakfast?

Author: Birgit Schelde Jensen, Jens Sandahl Christiansen, Bo Martin Bibby, Thomas Damgaard Sandahl, Tina Parkner, and Jannik Kruse Nielsen
Subjects: Adult, Blood Glucose, medicine.medical_specialty, Calorie, Daily intake, medicine.medical_treatment, Medicine (miscellaneous), Type 2 diabetes, Gastroenterology, Young Adult, Internal medicine, medicine, Humans, In patient, Glycemic, Aged, Nutrition and Dietetics, Cross-Over Studies, business.industry, digestive, oral, and skin physiology, Type 2 Diabetes Mellitus, food and beverages, Feeding Behavior, Middle Aged, medicine.disease, Crossover study, Diabetic diet, Endocrinology, Diabetes Mellitus, Type 2, Glycemic Index, business, Energy Intake, Food Deprivation
Abstract: To evaluate if an improved daily glycemic profile could be achieved in patients with type 2 diabetes by withholding breakfast, but maintaining the same total daily intake of calorie and the same composition of carbohydrates, fat and protein. Thirteen type 2 diabetic patients participated in this randomized crossover study. Following an initial fasting night the study consisted of 4 consecutive days. Patients were randomized to diets including breakfast days 1 and 3, and excluding breakfast days 2 and 4, or vice versa. The mean plasma glucose level was 0.24 mmol/l higher after the breakfast diet compared with the non-breakfast diet, but reflected only a tendency (P=0.066). The standard deviation based on plasma glucose was 32% higher after the breakfast diet compared with the non-breakfast diet (P
Published: 2011
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32. Obesity does not influence the unique pharmacological properties of different biphasic insulin aspart preparations in patients with type 2 diabetes

Author: S. E. Dyrskog, T. Lauritzen, J. W. Chen, Tina Parkner, L. Brondsted, Torben Laursen, Jørgen S. Christiansen, U. Mouritzen, and Kjeld Hermansen
Subjects: Adult, Male, medicine.medical_specialty, Time Factors, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Type 2 diabetes, Drug Administration Schedule, Body Mass Index, Insulin aspart, Endocrinology, Pharmacokinetics, Double-Blind Method, Diabetes mellitus, Internal medicine, Internal Medicine, Medicine, Humans, Hypoglycemic Agents, Insulin, Insulin Aspart, Aged, Glycated Hemoglobin, Cross-Over Studies, business.industry, Area under the curve, Middle Aged, medicine.disease, Crossover study, Hypoglycemia, Treatment Outcome, Diabetes Mellitus, Type 2, Pharmacodynamics, Female, business, medicine.drug
Abstract: Udgivelsesdato: 2010-May AIM: To investigate the influence of obesity in type 2 diabetic patients upon pharmacological properties of different biphasic preparations of insulin aspart. METHODS: A total of 75 type 2 diabetic patients were stratified according to their body mass index (BMI) into 40 non-obese (BMI 23-28 kg/m(2)) and 35 obese (BMI 30-35 kg/m(2)) subjects. The trial was a double-blinded crossover study. In two periods of 4 weeks each the patients received subcutaneous injections of biphasic insulin aspart 50 (BIAsp 50) or biphasic insulin aspart 70 (BIAsp 70) thrice daily in random order. Insulin doses were titrated individually. At the end of each period 24-h serum profiles of insulin aspart, C-peptide and glucose were recorded. The primary endpoint was the area under the curve of serum glucose concentration during 24 h (AUC(Glu)(0-24 h)). RESULTS: The insulin concentration profiles of BIAsp 50 and 70 were as expected according to the content of protamine-bound insulin aspart (50 and 30% respectively). AUC(Glu(0-24 h)) BIAsp 50/BIAsp 70 ratios were 0.97 (95% CI: 0.90-1.05, p = 0.49) for non-obese and 0.98 (95% CI: 0.92-1.05, p = 0.55) for obese. Fasting serum glucose (FSG) BIAsp 50/BIAsp 70 ratios were 0.90 (95% CI: 0.84-0.96, p = 0.002) for non-obese and 0.90 (95% CI: 0.84-0.97, p = 0.006) for obese. During both treatment regimens the frequency of minor hypoglycaemic episodes was highest for the non-obese group. CONCLUSIONS: The pharmacokinetic and pharmacodynamic characteristics of the two preparations of biphasic insulin aspart were different; however, they were not influenced by the degree of obesity in type 2 diabetic patients.
Published: 2010
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33. Overnight versus 24 hours of continuous subcutaneous insulin infusion as supplement to oral antidiabetic drugs in type 2 diabetes

Author: J. S. Smedegaard, Tina Parkner, Torsten Lauritzen, Jian-Wen Chen, Marianne K. Møller, Torben Laursen, Christina Jørgensen, Jens Sandahl Christiansen, and Henrik Frederik Thomsen
Subjects: Insulin pump, medicine.medical_specialty, insulin analogue, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Biomedical Engineering, 030209 endocrinology & metabolism, Bioengineering, Type 2 diabetes, Insulin aspart, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Internal Medicine, medicine, 030212 general & internal medicine, Glycemic, business.industry, Insulin, CSII, Original Articles, insulin aspart, intermittent insulin pump, medicine.disease, Subcutaneous insulin, Postprandial, Endocrinology, Basal (medicine), insulin pump, type 2 diabetes, business, medicine.drug
Abstract: Basal continuous subcutaneous insulin infusion (CSII) therapy at a fixed rate may effectively improve glycemic control in patients with type 2 diabetes when oral antidiabetic treatment fails. Regimens of simple constant subcutaneous delivery of insulin may provide theoretical advantages in type 2 diabetes.Ten subjects with type 2 diabetes who obtained insufficient glycemic control on oral antidiabetic drugs were included. Following an initial control day, two periods of 3 days with CSII of a rapid-acting insulin analogue, 1.5 IU/h (dose obtained from a preceding study), for 8 hours overnight and for 24 hours, respectively, were carried out in random order. Profiles of serum insulin aspart, serum endogenous insulin, and plasma glucose were recorded.Compared to the control day, an 8-hour overnight insulin infusion during a 3-day period improved fasting plasma glucose (FPG) (mean differences +/- SEM; Delta59.0 +/- 10.1 mg/dl; p0.01) and 2-hour postprandial plasma glucose (PPPG) (Delta57.8 +/- 10.6 mg/dl; p0.01) after breakfast. Compared to an 8-hour overnight infusion, a 24-hour infusion further improved all three PPPG values after breakfast, lunch, and dinner (Delta28.8 +/- 8.1 mg/dl, Delta30.6 +/- 8.1 mg/dl, and Delta35.1 +/- 7.9 mg/dl; p0.01). During insulin infusion, only one hypoglycemic episode with PG55.8 mg/dl and mild symptoms was recorded.Continuous subcutaneous insulin infusion with a rapid-acting insulin analogue at a fixed rate of 1.5 IU/h, either overnight or for 24 hours, improved glycemic control without safety concerns in patients with type 2 diabetes who had secondary failure to oral antidiabetic drugs. The effect on FPG was similar for both treatments, whereas the effect on PPPG was superior when insulin was infused during the entire 24 hours.
Published: 2007
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34. Overnight CSII as supplement to oral antidiabetic drugs in type 2 diabetes

Author: Christina Jørgensen, T. Lauritzen, M. K. Møller, Torben Laursen, Jørgen S. Christiansen, J. W. Chen, J. S. Smedegaard, and Tina Parkner
Subjects: Insulin pump, Adult, Blood Glucose, medicine.medical_specialty, Insulin Analogue, Adolescent, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Injections, Subcutaneous, Enzyme-Linked Immunosorbent Assay, Type 2 diabetes, Drug Administration Schedule, Body Mass Index, Insulin aspart, chemistry.chemical_compound, Endocrinology, Insulin Infusion Systems, Internal medicine, Internal Medicine, medicine, Humans, Hypoglycemic Agents, Insulin, Insulin Aspart, Morning, Aged, C-Peptide, Dose-Response Relationship, Drug, business.industry, C-peptide, Middle Aged, medicine.disease, Treatment Outcome, chemistry, Diabetes Mellitus, Type 2, Drug Therapy, Combination, business, Epidemiologic Methods, Body mass index, medicine.drug
Abstract: Udgivelsesdato: 2008-Jul AIM: To evaluate the potential advantages of a constant overnight subcutaneous delivery of insulin in type 2 diabetic patients who fail to achieve glycaemic control on oral antidiabetics. METHODS: Ten type 2 diabetic patients treated with oral antidiabetic drugs without gaining sufficient glycaemic control were included in this three-period study. All patients received continuous subcutaneous insulin infusion (CSII) with a short-acting insulin analogue, 2 IU/h, for 8 h during three consecutive nights (period A). Based upon the results from period A, two additional dose regimens of three nights (period B and C) were studied in random order. Serum insulin aspart, human insulin and plasma glucose (PG) profiles were recorded. RESULTS: In period A, fasting plasma glucose (FPG) was reduced from a mean +/- s.d. (mmol/l) value of 11.6 +/- 2.9 to 5.5 +/- 1.6 (p < 0.0001) during the first night. No additional lowering of FPG was seen the two succeeding nights. FPG narrowed as the range before the infusion was 7.3-15.2 mmol/l compared with 3.6-6.1 mmol/l on the last morning after infusion. The variability in PG profile during the first and the last night of CSII was small and not significantly different. The rising insulin aspart was mirrored by a decrease in human insulin. In period B and C, similar tendencies as for period A were seen. In period A, two patients each experienced one mild hypoglycaemic episode. CONCLUSIONS: CSII with an insulin analogue overnight effectively reduced FPG without occurrence of major hypoglycaemia in type 2 diabetic patients who fail to achieve glycaemic control on oral antidiabetic treatment.
Published: 2007
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35. 40(th) EASD Annual Meeting of the European Association for the Study of Diabetes : Munich, Germany, 5-9 September 2004

Author: S. Artigas, A V Dreval, Mark I. McCarthy, C Watson, Peter H. Bennett, M Quint, Y Ikeda, E Alpert, F Schiele, H Sekihara, Erik Gylfe, P Lowe, J Kuhlmann, Alain Golay, V Longo, Shahidul Alam Khan Akm., L G Mantovani, M Zawodniak-Szalapska, G Winkler, T Harrity, L Virág, U Johne, Kuo S-W., Linda C Tapsell, J Rodriguez, Michel Komajda, K Kankova, Carole A. Cull, M Sporna, E Estilles, U Ribel, M C Spruce, E Buzzigoli, T Prazak, J K McLaughlin, M K Lingohr, M Lim, F Calara, A Siebenhofer, G Meregalli, Roberto Anichini, A D Baron, R Kurashvili, P C Butler, G I Fantus, T. E. De Gooyer, Park Y-M., R. Walther, S Heinrich, Agnieszka Zawiejska, S Mukherjee, Nikolaos Papanas, G Wong, Ian D. Caterson, David M. Maahs, Shuichi Kaneko, Alexandra E. Butler, Francisco Javier Ampudia-Blasco, O N Kong, Attali J-R., C A Hedman, K Oshinyemi, Nicolle Müller, I C Cranston, N Okumus, M V Vlaiculescu, Balasubramanian Ravikumar, W W Cheatham, K Mukasa, K B Biswas, Annunziata Lapolla, Phil McEwan, G Mader, Gilles Chassot, Dragi Anevski, Werner A. Scherbaum, M Donath, C Hesselmann, R A Gandhi, David E. Moller, Ezio Bonifacio, C Garcia, V Ifandi, P Hornnes, Nieuwenhoven Fav., C Puech, S Pérez-Del-Pulgar, Kim S-R., G Hines, C Rubio Terrés, Michael Gaster, N. Hosszufalusi, A Scholze, Andrew A. Young, Stavros Liatis, F Hariri, S Tan, Paul Valensi, Allan E. Karlsen, J Kim, E. Moberg, J Kaiser, L Berman, G Nelson, A Altkrüger, P Kothare, D B Cook, S Doran, G. van Dijk, Shahnaz Shahinfar, Kim C-S., P Stahl, M Manousaki, S Sigrist, S K Lim, M. P. Stern, A Guberti, C Rezzani, J McKenney, Karl Thomaseth, Sofia Carlsson, M Julia, R Brillante, I Rubesova, T Darkow, E Matsumoto, Wendy M. Macfarlane, M Di Martino, G Bardini, Rossella Menghini, D Duhot, E Farcasiu, Annalisa Natalicchio, I Lindner, J Buvat, Christian L. Brand, Harry Dorchy, Iwona Pietrzak, Z T Luo, P Home, M Ekelund, Jesper Gromada, Kristine Færch, F Piarulli, H Kim, R Mentel, Zsuzsanna K. Zsengellér, Dullaart Rpf., Anton Luger, Thomas A. Pearson, V Manicardi, P Rösen, Feng Y-M., R Morganti, Lars Hansen, Demuth H-U., Haruo Kasai, A Shostak, Rudi Steffensen, G Taylor, Markolf Hanefeld, C Santini, E Hamaguchi, Roberto Miccoli, F Storms, M Cooper, Y Lee, Allison E. Aiello, P Smith, T Suehiro, K Treece, M Waluś, Timothy A Welborn, Simone Baltrusch, E Kontela, S Chai, J Crean, H Yokoyama, Johan G. Eriksson, Rafael Hernández Hernández, J Rodríguez-Saldaña, M P Tornero, G Formoso, D. Lovell, E Bingham, A Mylonakis, M Manteghetti, D Fedele, Antonio Martín-Duce, Ralph A. DeFronzo, D Salcedo, Kurt Højlund, Antonio Petrone, Sheu Whh., C Gutierrez, Flavia Pricci, S Kurita, Z G Abbas, M M Benedetti, Philippe A. Halban, Daniel J. Cox, O Ljungkvist, Justine Davies, J Palsgaard, Lars Sjöström, E Bosi, L Janin-Manificat, W. F. Kelly, M. Fernandez, E Colak, O V Mulyarchik, B Kronshage, F Lang, M Erfurth, Takashi Kadowaki, N Jendrike, U Walter, J Wishart, Y. Neye, D Kim, N Furuhashi, M Barsotti, D Florow, L Ke, L Borgquist, N C Jackson, Ffolliott M. Fisher, V Baskar, K Yoshioka, Bryan A. Wolf, G Chabrier, R Skoumal, Livio Luzi, H Kose, I Pharisien, B. Klein, H Winiarska, M C Johnson, L Griffiths, Nonna Kravchun, C Combe, Baptist Gallwitz, J Zdychova, L Skorda, Jorma Ilonen, W Gao, I N Steen, A Terrinoni, P D Ambery, W Kern, C M Kusminski, Cho M-H., Paolo Pozzilli, Louise G. Grunnet, E Schönle, David R Matthews, Robert W. Taylor, Y Cohen, Kim H-S., M P Eccles, N B Tutuncu, D McDowell, Richard M. Bergenstal, K Takamatsu, T Steiner, Jaan Palgi, Valdemar Grill, N Niculescu, G Federici, S Lehto, P. M. McKeigue, M Barone, Michael E. Trautmann, S Smirnov, J Mannion, M Eto, C Rousseau, M Conti, C S Ernest, Antonio Ceriello, D H Schweitzer, Jung E-D., Andreas Festa, Avijit Lahiri, A Shepelkevich, A Murro, A Kollmann, Jonathan R.S. Arch, R Landgraf, Son H-Y., I Engelsberger, E Agardh, S Rodríguez-Mulero, P J Kraml, K Lee, D. F. Du Toit, E Kim, G Fadini, Williams Ajk., Philip Home, M B Antcieferov, C Perlemoine, D Perrea, Song X-L., D Ruggieri, Krister Bokvist, Heidi Sørensen, Bilbao, G Yoshino, J P Taylor, Shen H-M., S M Furier, R Urquhart, J Wohlgelernter, Jianping Weng, T. Baba, Q Hong, C Silva, Castaigne J-P., M Felaco, X X Zhang, M Jaroň, Milla Rosengård-Bärlund, J G Papp, Toshio Miyata, Lervang H-H., Park M-K., I Kinalska, A Long, Oomen Phn., N Kogawa, Ippolita Patrizia Patera, S. Karadeniz, Dinesh Selvarajah, D S Chung, A Wensaas, Richard Imrich, M Recasens, J Ruxer, O Buchea, E Wilpart, S P Stepanenko, Le Ttd., H Ohgawara, Mariaconsuelo Valentini, A Mondok, M Peltonen, Marianne O. Larsen, K Chatzianagnostou, Agneta Ståhle, A L Ferrari, L Bordier, F Maingrette, A Matsuda, G Vukomanovic, Jakob D. Wikstrom, T Yamakita, E Gorostiaga, J Jin, B Gopalan, Heinz Drexel, S Hewitt, Rury R. Holman, C Dieterle, T L Ruchti, N Asatiani, M Sidira, A Iezzi, A J Sommerfield, D Châtenet, M L Olsen, R Bergemann, C Koehler, T L Kuraeva, B Balas, Christian Berne, E Santos-Mazo, G Smith, A Siejka, R Kožnarová, A Mattina, S Sheikh, A Adomeit, M Rasmussen, J. Fagerudd, N Busciantella Ricci, Nuria Vilarrasa, E Hammar, T L Thoms, L Aydın, Ron G. Rosenfeld, A Nikolajuk, R Gos, C L Morgan, H L Yu, D Dheelchand, S Ramrath, N Boudriga, Jerome I. Rotter, C Jahannault, W M Weston, Folke Lindgärde, M Hertlova, D Knight, A Monroy-Mayorga, E Pardini, A Chamson-Reig, B Franke, Janie McCluskey, Joseph Bryan, C Nikolopoulou, Christie M. Ballantyne, Fausto Santeusanio, L Pegoraro, M Lee, A Klimenko, S Jaiveer, K. Pettersson-Fernholm, Michael A. Nauck, A Ekbom-Schnell, G Deferrari, Riccardo Schiaffini, S. Pampanelli, Khan Aka., David Hopkins, Maija Wessman, M Kamarinos, Noh J-H., O Ebisui, K McCarroll, Jeppe Sturis, Peter Nowotny, N Gorbenko, Åke Sjöholm, David G. Maggs, A E Halseth, B Cresci, A A Ortiz-Gress, A Korakovouni, O Matejkova, C E Mogensen, C J Lin, Ramon Gomis, H Seaman, C Granier, Yang C-H., F Assah, O Sanchez, Fausto Machicao, Peter G. Morris, Alberto Ortiz, A Giardinelli, D Bracaglia, A Gonzalo, S Pavlatos, Andreas Lechner, F Canovic, L Sjolind, Allan Vaag, Birgitte Bruun Nielsen, David A. Ziegler, Vito Lampasona, R Gershoni-Baruch, A. Dei Cas, H Renz, E Mena, Matthew Waltham, Kim D-M., H Levanen, D D Mick, Valentina Alexandrovna Peterkova, E Meskhishvili, Sarah Nutland, R Bustani, John R. Lindsay, M Christoforidou, A Abicht, E Harno, K Cyganek, A Fitchet, S Neelotpol, P Nikishin, P Serradas, J Hinrichsen, M Halvorson, M Chovatia, B Voet, Jinny Willis, E Parretti, M Haslbeck, M Wellard, L Teng, Julio Wainstein, J S Fischer, K. Lalic, D Roggenland, I Gich, R Anwar, Maurizio Cassader, D Serota, X J Li, R J Schotzinger, Vilmundur Gudnason, Björn Zethelius, S A Wootton, W Andrzejewski, R Rezsohazy, R Gao, T Klimentova, T Mazurek, I Bruckner, C Dohrmann, R E James, G daSilva Xavier, Kim S-Y., A Dorca, Stuart J. Pocock, Terri J. Allen, I Giovos, P B Parab, N H Andersen, P Fotinakis, Miriam Cnop, H Lee, Norbert Tennagels, Omorodola I. Abatan, F Ailett, I. Lager, D Manzella, H Hut, Larry A. Distiller, G Lip, Lim S-K., Rong Zhang, T Tsuno, Steen Knudsen, M. Bajardi, Manuel Benito, Dai Sugimoto, Melvin J. Prince, D W Dunstan, D Rankins, K A Majali, G Ozansoy, Isabella Russo, S Uçak, G Annuzzi, R Talar-Wojnarowska, K Lange, S Neugebauer-Baba, Campbell H. Thompson, Eric Renard, P. D. Mountjoy, Z Morrison, Elizabeth A. Davis, Franco Cavallo, C Corvaja, R Antuña, Craig John Currie, H Linnebjerg, He Y-L., A J Palmer, Mariola R. Chacón, H Malinska, M. Jones, R Lichnovská, K Mandes, Paolo Tessari, T Mokhort, A Laina, H. L. Y. Chan, I Schmidt, R Banks, Richard G. IJzerman, L Ksinantova, G Setti, H Vaudry, A Gallo, V Spallone, Chen J-W., Thomas Danne, A Chong, M Hallschmid, S Aczel, S Hulme, N Islam, M Hosoi, P M Ternan, P Di Bartolo, N Bishara, T Shibasaki, Martin A. Osterhoff, Im S-S., M Jecht, T Hamaguchi, S Mattera, K Ways, Elizabeth Northam, U Rajala, Reinhard W. Holl, L Yang, S Panaiotopoulos, K Horvath, R Kluge, Thora B. Bodvarsdottir, Y Dong, Irene Alemanno, C McDougall, Reimar W. Thomsen, M Campbell, W Rabl, John Öhrvik, Yuichiro Yamada, Paola Ungaro, W Benzer, Mike Sampson, Roberto Trevisan, R G Radu, Aas A-M., P E Lobo, Ricardo Scott, S M Son, Josephine M. Forbes, T A Hillier, K L Wyne, Louis L. Nguyen, J Farmer, M H Tan, Kwon H-S., J Yang, L Sandvik, Franco Folli, A K Jenum, M Nguyen, W Pratipanawatr, A L Frederiksen, Rebecca Smith, Lee H-J., A Schäfer, C Manuelli, G S Denver, T Vukovich, B Maceira, K Matsumoto, K. Chokkalingam, Nurcan Üçeyler, P Modi, Timothy M. Morgan, S Mertens, B M Singh, Michaela Riedl, K Iso, C Cucurullo, G. F. Bottazzo, M Calvani, K Hur, J Wetzels, Kazuhiro Takahashi, Y Aso, H Stammer, M G Masding, Fitsum Guebre-Egziabher, J L González-Sánchez, L Armstrong, Alberto Maran, Peter G.F. Swift, S S Popovic, J Starczynski, E Vitacolonna, Luigi Laviola, R W Gelling, Marina Cardellini, D Barilla, Rosa de Diego Martínez, W H Landschulz, Anne Mette Rosenfalck, R K Wong, Kevin E. Schneider, K Peros, Giuseppe Nanni, F Zhang, I Rákóczi, T Iburi, M Nakhjavani, X Q Zhang, S Tournis, Per Lav Madsen, Graham A. Hitman, A. Tura, K Laubner, N D Kostic, Lawrence M. Dolan, R. Sinha Roy, J A Wagner, J. Tuomilehto, J Hauptman, M Abdel-Ghany, D Lacombe, Toralph Ruge, Johannes A Maassen, Triantafyllos Didangelos, K Sasaki, I Argüelles, Klaus Levin, C Popow, Emanuel Christ, R Chetty, L Baillet-Blanco, Jo-Ann Salmon, T Mine, James L. Trevaskis, I Franke, J Gorski, E A Andrianova, A Dayan, A Caballero, Aleksandra Gilis-Januszewska, M Yasujima, Z Kasalová, C.D.A. Stehouwer, F. K. Gorus, G A Nichols, A Glowania, David P. Strachan, P Fredlund, N. F. da Silva, P Reboldi, M Sausbier, K H Groenier, G Stuccio, N Guttman, K R Ahmed, A D Ristic, T Kapellen, J Coutcher, Aldo V. Greco, Oswald Wagner, A Zagayko, Maria Alevizaki, B B Zhang, W F Ferris, Jenny Fredriksson, Lois Jovanovic, J Hänninen, R De Giglio, Kazuo Yagui, O Potterat, P Hamliton, R E Scranton, B Mankovsky, A Stylianou, B Fellström, Abdel-Wahab Yha., M Kitagawa, Katherine L. Baldock, F R Johnson, F Baigts, S D'Addato, F J Sanz, A Mistry, S D Wise, T Pratipanawatr, U R Fölsch, James R.C. Parkinson, Claudia Sommer, C Park, F E Griffiths, M L Martí, R Demirtunc, S Taniguchi, J Lundkvist, T Siegmund, Juan Sztajzel, C Dienesch, F Baumgartner, L Scalone, T M Mckolanis, K Otake, Ullrik Pedersen-Bjergaard, T M Vriesendorp, Michael B. Wheeler, Henry Schmitt, Peter Hovind, S Lange, Stephane Roze, L. Van Gaal, B Klaproth, Anthony E. Civitarese, D Eckland, A Dagar, D F Hopkins, Kari Stefansson, C Gonzalez-Yanes, B Meyboom-de Jong, D. J. Betteridge, K Buhling, M Crepaldi, Ana M. Wägner, L Renna, L Volpe, R McBride, V Corbo, E O Brennesvik, R P Hayes, R Abdollahnia, G Viviani, C F Liew, Francisco Pérez-Bravo, Jeffrey Baron, Brian M. Frier, H H Samira, D Szentendrei, K. J. Schjoedt, W K Waldhäusl, D Gniuli, D Zou, G Tschank, V Urbančič, A L Nolan, Albertini J-P., J Malcomson, M Larbig, C Cheyssac, K Aurich, C M Kesson, S Heller, Maija E. Miettinen, R F Luco, Adrian J. Cameron, Luigi Mattiello, Z. Metelko, X E Zhang, M Parramón, I. G. Obrosova, J Fruchart, M Ilic, Björn Eliasson, Gilles Chatellier, M A Martín, D M Kendall, Holger Luthman, V F Varillas, D Maccubbin, Jang S-A., Amalia Gastaldelli, E Salzsieder, P. de Mol, A Yoshida, H D Lindner, D Gostiljac, M Just, Pan C-Y., J M Fujitaki, G Eiermann, K Bergenheim, A D Frick, A Agacdiken, K Varytimiadis, K Cseh, D A Jackson, S Calderari, Dena G. Hernandez, H M Liebich, K Min, F. de Zegher, Bernd Kulzer, K Han, Ulrich A. Müller, D Marrero, H Hatakeyama, René Koopman, Doo H-K., Petr Wohl, P. Sharp, P Forder, Thor Aspelund, N Meneveau, R M Schmülling, R Aubert, Thom Sam., H Youshikawa, M Ankelo, D Bowden, I Kelly, Frédéric Fumeron, M Sartini, Robert S. Sherwin, L Varadhan, A Criscimanna, John Betteridge, V Jelic, M Bartnik, N Lemke, B Ursø, A Bertoldo, A M Owona, H Okochi, L Pérez-Tamajó, S L Monfre, Daniel Brandhorst, K T Legg, Andries J. Smit, Veronica Sancho, Masashi Hirai, C Klein, Paul J. 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Ahmann, E Jebens, J Keiding, Elena Henkel, Mark Fineman, J F McRae, Carol Forsblom, S Martemucci, Lourdes Ibáñez, P G Prieto, L Ringholm Nielsen, S Pratas, B von Stritzky, Julio Rosenstock, Lee K-W., J Stocks, L J Strow, I Samarguliani, L Wennekes, R Cheung, Abhishek Nag, Roberto Gambino, Y Suleymanoglu, E Murphy, T T Durck, M F Peyrot, Y Unno, Alexander Mayorov, Eleuterio Ferrannini, D. C. Rao, D Neely, H Karunajeewa, J Palmisano, Julia B. Lewis, M Ravid, G Pons, E Junca, P Vexiau, S Sailesh, D K Miloslavskiy, O N Bondarenko, U Smith, S Torri, Constantine Tsigos, Cesario Bianchi, Mattia Locatelli, D Jaquet, Virpi Lindi, M Moroi, M E Tushuizen, P Pelicci, R Scognamiglio, Pal Pacher, S M Thyssen, A Péterfalvi, Y Ho, S Guntram, L Romics, T Nakagami, Clive S. Cockram, Irina Kowalska, K Brodbeck, Gojka Roglic, J. Dörig, Lise Tarnow, Therese Tillin, A López-Alba, Martin Krššák, Moses Elisaf, S Hata, D P Snoeck, D Schmoll, O V Udovichenko, A Scaramuzza, J Paul, John H. 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L, Villela, N, Laflor, C, Conde, C, Bottino, D, Dorigo, D, Bouskela, E, Pu, S, Luo, Z, Lam, K, Dan, Q, Xu, A, Shen, J, Cheng, K, Xu, J, Thamer, C, Stefan, N, Haap, M, Heller, E, Tschritter, O, De Prado, A, Ortiz, A, Ybarra, J, Gich, I, Pou, J, Ehren, M, Roggenland, D, Reinsen, B, Klein, H, Rittig, K, Stock, J, Kocher, B, Balletshofer, B, Shon, H, Chung, D, Nakatani, Y, Matsuhisa, M, Kaneto, H, Hatazaki, M, Yoshiuchi, K, Katakami, N, Kawamori, D, Ohtoshi, K, Sakamoto, K, Matsuoka, T, Ozawa, K, Ogawa, S, Hori, M, Yamasaki, Y, Zitouni, K, Harry, D, Nourooz zadeh, J, Earle, K, Olesen, P, Franco, L, Corvaja, C, Semplicini, A, Ceylan işık, A, Arı, N, Rösen, P, Lee, I, Park, K, Jung, E, Shin, D, Jo, S, Obuobie, K, Prakash, P, Hanna, F, Lazarus, J, Varadhan, L, Gurushankar, J, James, D, Sheikh, S, Gaede, P, Zou, D, Vilarrasa, N, Perez maraver, M, Mena, E, Perez, D, Setti, G, Buckingham, R, Urbančič, V, Stefanovska, A, Bernjak, A, Ažman juvan, K, Kocijančič, A, Glowania, A, Filters, T, Fosmark, D, Torjesen, P, Kilhovd, B, Berg, T, Sandvik, L, Hanssen, K, Mentink, C, Donchenko, G, Stepanenko, S, Maingrette, F, Deng, H, Lindenmair, A, Freudenthaler, A, Baumgartner parzer, S, Nizheradze, K, Khoruzhenko, A, Tronko, N, Sheu, W, Ou, H, Shen, H, Lin, T, Wu, H, Yang, C, Mogylnytska, L, Schmoelzer, I, Davies, J, Band, M, Struthers, A, Prázný, M, Škrha, J, Kasalová, Z, Neelotpol, S, Jahan, P, Kauschke, S, Harrop, C, Schäfer, A, Widder, J, Eigenthaler, M, Walter, U, Uchimura, I, Ikebukuro, M, Kaibara, M, Hirata, M, Helal, R, Pervin, F, Yang, X, Jansson, P, Nagaev, I, Jack, M, Carvalho, E, Sunnerhagen, K, Cam, M, Cushman, S, Smith, U, Creely, S, Farmer, J, Gustafson, B, Kusminski, C, Krusinova, E, Wohl, P, Klementova, M, Lanska, V, Mcdougall, C, Kelly, I, Abbas, Z, Lutale, J, Archibald, L, Karunajeewa, H, Stingemore, N, Stuccio, G, Mcgechie, D, Muller, L, Hak, E, Goudzwaard, W, Montorsi, F, Homering, M, Sprenger, K, Goldstein, I, Asnaghi, V, Ferrari, G, Rastaldi, M, Gabellini, D, Dell'Antonio, G, Maestroni, A, Ruggieri, D, Luzi, L, Piemonti, L, Zerbini, G, Anafaroglu, I, Tutuncu, N, Sultana, M, Siddiqua, N, Iwasaki, T, Nakajima, A, Yoneda, M, Mukasa, K, Tanaka, S, and Sekihara, H
Subjects: 0303 health sciences, medicine.medical_specialty, business.industry, EASD, Endocrinology, Diabetes and Metabolism, Human physiology, medicine.disease, 03 medical and health sciences, 0302 clinical medicine, Diabetes mellitus, Family medicine, Internal Medicine, Medicine, business, 030217 neurology & neurosurgery, 030304 developmental biology
Published: 2004

36. P02-46 Diurnal variation in the pharmacokinetics of growth hormone administered as a continuous subcutaneous infusion in GH-deficient subjects

Author: T. Lauritzen, Torben Laursen, Jurgita Janukonyte, J. S. Christiansen, and Tina Parkner
Subjects: medicine.medical_specialty, Endocrinology, Pharmacokinetics, business.industry, Endocrinology, Diabetes and Metabolism, Internal medicine, Diurnal temperature variation, medicine, Growth hormone, business, Continuous subcutaneous infusion
Published: 2012
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37. Rhodopsin in Serum – a Biomarker for Diabetic Retinopathy

Author: Eva Rabing Brix Petersen, Ivan Brandslund, Hansen, Søren B., Tina Parkner, and Cramer Christensen
Subjects: genetic structures, sense organs
Abstract: Rhodopsin in Serum – a Biomarker for Diabetic Retinopathy

38. Pharmacokinetics following continuous subcutaneous insulin infusion of insulin aspart with or without initial subcutaneous bolus

Author: Esben Søndergaard, T. Lauritzen, Signe Beck Petersen, Jonas Kildegaard, Tina Parkner, Jørgen S. Christiansen, Flemming S Nielsen, Ole Skyggebjerg, and Torben Laursen
Subjects: Adult, Male, medicine.medical_specialty, Time Factors, Adolescent, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Body Mass Index, Insulin aspart, Young Adult, Endocrinology, Bolus (medicine), Insulin Infusion Systems, Pharmacokinetics, Internal medicine, Healthy volunteers, Internal Medicine, medicine, Humans, Hypoglycemic Agents, Insulin, Insulin Aspart, Glycated Hemoglobin, Cross-Over Studies, business.industry, Basal insulin, Fasting, Crossover study, Subcutaneous insulin, Treatment Outcome, Female, business, medicine.drug
Abstract: Udgivelsesdato: 2010-Apr AIM: To evaluate time to steady state insulin concentration (C(ss)) following continuous subcutaneous insulin infusion (CSII) of insulin aspart (IAsp) with or without an initial s.c. bolus. METHODS: In random order 10 healthy volunteers were given a basal insulin infusion rate (0.5 U/h) for 8 h with or without an initial s.c. bolus (1.4 U). Serum IAsp was measured until 3 h after infusion was stopped. RESULTS: An overshoot of IAsp was seen before C(ss) was achieved following an initial bolus of insulin as compared to no bolus. The apparent half-life (t((1/2))) with or without bolus did not differ (p = 0.15). Time to steady state (T(ss)) was evaluated in two ways: (1) T(ss) defined as the first point within an interval of C(ss)+/- 2 x CV was 233 vs. 166 min with and without a bolus respectively (p = 0.068). (2) A t-test was performed for each concentration-time point vs. mean C(ss), and the first point with no significance was defined, T(ss). This gave 208 (p = 0.09) and 178 min (p = 0.24) with and without bolus respectively. Mathematical modelling suggests that an ideal mean bolus should be 0.89 U, and that this bolus dose may result in a shorter T(ss). CONCLUSION: A bolus of 1.4 U resulted in an overshoot of serum IAsp before C(ss) and a longer period before C(ss) is achieved. Mathematical modelling suggests that a mean bolus of 0.89 U would result in a faster achievement of C(ss) compared to no bolus.
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