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Background: Cambodian refugees arrived in the U.S. with limited English proficiency, low educational attainment, poor health status, and significant trauma. Combined with poor healthcare access, this fostered an environment for continued reliance on cultural health practices. Maternal health, including the postpartum period, was particularly influenced by traditional Cambodian medicine. This study investigated associations between sociodemographic factors and Cambodian women’s knowledge, beliefs, and practices related to sra thnam, a traditional postpartum medicine used in the Cambodian diaspora. Methods: This cross-sectional study was conducted with Cambodian women ages 15-35 years (N=162) living in Massachusetts. A community-academic team developed study materials and administered surveys and focus groups on knowledge, beliefs, and use of sra thnam. Focus group discussions were entered into NVivo 10. Survey data was analyzed using Stata version 14.0 and SPSS version 27. A series of statistical analyses were performed to assess associations between participant use of sra thnam and socio-demographic, health, and psychometric factors. Results: Knowledge of sra thnam was almost universal (92%), with 44% these participants reporting ever consuming it. Pregnant women (N=56) reported not having consumed sra thnam during the current pregnancy and after childbirth, 52% reported an intention to consume sra thnam, 42.9% did not, and 5.4% were uncertain. After adjusting for covariates, being born in the U.S., having at least one child, older age, and larger households were predictive of ever consuming sra thnam. Conclusion: These findings show traditional postpartum knowledge and practices are common among Cambodian women living in the U.S. These practices may have benefits, as well as pose some risks, for maternal health. Given that perceptions of pregnancy, childbirth, and postpartum experiences are nested in culture, it is important that healthcare providers are aware of traditional health beliefs and practices in refugee and immigrant communities. Greater understanding of traditional postpartum practices can facilitate culturally relevant support and health care services to mothers.

Ethnopharmacological relevance Evidence of phytochemical roles in infant development and maternal recovery offers insights into beneficial functions of traditional plant use during lactation and the postpartum period. Ethnopharmacological research has relevance to global priorities on maternal and child health, to understanding origins and determinants of human self-medication, and for reconciling traditional postpartum practices and mainstream healthcare. Aim of the study Present emerging evidence, within evolutionary and socio-cultural contexts, on the role of maternal consumption on transfer of phytochemicals into breast milk with impacts on maternal and child health, and on infant development. Establish current state of knowledge and an ethnopharmacological research agenda that is attentive to cross-cultural and regional differences in postpartum plant use. Materials and methods An extensive literature review using Medline, Scopus, and Web of Science focused on traditional and contemporary use and socio-cultural context, as well as physiological, pharmacological, toxicological, and behavioral activities of plants used medicinally by women during postpartum recovery and lactation. Results The most widely reported postpartum plants show antimicrobial, anti-inflammatory, immunological, and neurophysiological activities, with low toxicity. Phytochemicals transfer from maternal consumption into breast milk in physiological concentrations, while animal studies demonstrate immunomodulation and other actions of medicinal plants during lactation. Reporting on the use and diverse traditional knowledge of women about plants during the postpartum period is obscured by the marginal place of obstetric issues and by gender biases in ethnobotanical research. In many contemporary contexts use is prejudiced by precautionary risk warnings in health literature and practice that confound lactation with pregnancy. Conclusions Although systematic investigation of postpartum plant use is lacking, known pharmacological activities support potential benefits on infant development and maternal health with immediate and long-term consequences in relation to allergic, inflammatory, autoimmune, and other diseases. An ethnopharmacological agenda focused on the perinatal period requires directed methodologies and a regional approach in relation to culturally-specific knowledge and practices, traditional plant use, and local health needs. Testing the hypothesis that phytochemicals transferred from medicinal plants into breast milk impact the human immune system and other aspects of infant development requires extended analysis of phytochemicals in human milk and infant lumen and plasma, as well as effects on gastrointestinal and milk microbiome.

Seed security is complementary and relational to food security; having access to seed that produces meaningful and resilient yields of culturally appropriate food is an integral aspect of food security for smallholder farmers. However, essential components of smallholder seed security continue to be underemphasized in food and seed policy. In this study, we analyze household and farm-level characteristics that may predict chronic seed insecurity in semi-arid eastern Kenya. In the process, we also present and test the Household Seed Insecurity Assessment Scale (HSIAS) designed to measure household chronic seed insecurity. Results suggest that mild chronic seed insecurity continues to be a problem in most households, hampering their ability to produce food. We found that older and more experienced farmers were more seed insecure and that farmer adoption of new varieties was associated with seed insecurity. Obtaining seed through local markets and informal giving was done evenly by all farmers while using agroshops was associated with greater seed insecurity in some instances. Key attributes of household seed (in)security identified in this study are used to inform seed and food policies that better support smallholder farmers in Kenya. With further development, the HSIAS has the potential to enhance local monitoring systems and government food and seed policy responses.

Ethnopharmacological relevance Larix laricina , a native tree of North America, is a highly respected medicinal plant used for generations by Indigenous Peoples across its range, including the Cree of northern Quebec who use the bark to treat symptoms of diabetes. This study investigates the antioxidant capacity and bioavailability of active constituents identified in L. laricina bark extracts. Materials and methods (1) Oxygen radical absorbance capacity (ORAC) assay was employed to test antioxidant capacity of organic extracts (80% ethanol) from bark of L. laricina as well as fractions, isolated compounds, and media samples collected during permeability assays. (2) Caco-2 cell monolayer cultures were used to determine the permeability of identified antioxidants, which were quantified in basolateral media samples using liquid chromatography – tandem mass spectrometry (HPLC-ESI-MS/MS). Results Crude ethanolic extract possessed strong antioxidant potential in vitro (7.1±0.3 Trolox equivalents (TE) μM/mg). Among the 16 L. laricina fractions obtained by chromatographic separation, fraction 10 (F10) showed the highest antioxidant capacity (21.8±1.7 µm TE/mg). Among other identified antioxidants, the stilbene rhaponticin (isolated from F10) was the most potent (24.6±1.1 µm TE/mg). Caco-2 transport studies revealed that none of the identified compounds were detectable in basolateral samples after 2-h treatment with crude extract. In monolayers treated with F10 (60% rhaponticin), small quantities of rhaponticin were increasingly detected over time in basolateral samples with an apparent permeability coefficient (P app ) of 1.86×10 −8 cm/s (0–60 min). To model potential effects on blood redox status, we evaluated the antioxidant capacity of collected basolateral samples and observed enhanced activity over time after exposure to both extract and F10 (75 μg/mL) relative to control. Conclusions By profiling the antioxidant constituents of L. laricina bark, we identified rhaponticin as the most potent oxygen radical scavenger and observed low permeability in Caco-2 cell monolayers but an increase in basolateral antioxidant capacity.

Ethnopharmacological relevance Cross-cultural comparison of plants used during lactation and the postpartum period offers insight into a largely overlooked area of ethnopharmacological research. Potential roles of phytochemicals in emerging models of interaction among immunity, inflammation, microbiome and nervous system effects on perinatal development have relevance for the life-long health of individuals and of populations in both traditional and contemporary contexts. Aim of the study Delineate and interpret patterns of traditional and contemporary global use of medicinal plants ingested by mothers during the postpartum period relative to phytochemical activity on immune development and gastrointestinal microbiome of breastfed infants, and on maternal health. Materials and methods Published reviews and surveys on galactagogues and postpartum recovery practices plus ethnobotanical studies from around the world were used to identify and rank plants, and ascertain regional use patterns. Scientific literature for 20 most-cited plants based on frequency of publication was assessed for antimicrobial, antioxidant, anti-inflammatory, immunomodulatory, antidepressant, analgesic, galactagogic and safety properties. Results From compilation of 4418 use reports related to 1948 species, 105 plant taxa were recorded ≥7 times, with the most frequently cited species, Foeniculum vulgare, Trigonella foenum-graecum, Pimpinella anisum, Euphorbia hirta and Asparagus racemosus, 81, 64, 42, 40 and 38 times, respectively. Species and use vary globally, illustrated by the pattern of aromatic plants of culinary importance versus latex-producing plants utilized in North Africa/Middle East and Sub-Saharan Africa with opposing predominance. For 18/20 of the plants a risk/benefit perspective supports assessment that positive immunomodulation and related potential exceed any safety concerns. Published evidence does not support a lactation-enhancing effect for nearly all the most-cited plants while antidepressant data for the majority of plants are predominately limited to animal studies. Conclusions Within a biocultural context traditional postpartum plant use serves adaptive functions for the mother-infant dyad and contributes phytochemicals absent in most contemporary diets and patterns of ingestion, with potential impacts on allergic, inflammatory and other conditions. Polyphenolics and other phytochemicals are widely immunologically active, present in breast milk and predominately non-toxic. Systematic analysis of phytochemicals in human milk, infant lumen and plasma, and immunomodulatory studies that differentiate maternal ingestion during lactation from pregnancy, are needed. Potential herb-drug interaction and other adverse effects should remain central to obstetric advising, but unless a plant is specifically shown as harmful, considering potential contributions to health of individuals and populations, blanket advisories against postpartum herbal use during lactation appear empirically unwarranted.

Sorbus decora and Sorbus americana are used traditionally as medicine by the Eeyou Istchee Cree First Nation of the James Bay region of Quebec, Canada. Because the ethanol extracts of the bark and the isolated terpenes of these plants have shown promising in vivo antidiabetic effects, an analytical method was developed and validated by RP-HPLC-ELSD for the identification and quantification of eight lupane- and ursane-type terpenes. The extraction method reproducibly recovered the compounds above 70 % and the chromatographic separation of betulin, 23-hydroxy-betulin, 23,28-dihydroxylupan-20(29)-ene-3β-caffeate, betulinic acid, α-amyrin, uvaol, 3β,23,28-trihydroxy-12-ursene, and 23,28-dihydroxyursan-12-ene-3β-caffeate was achieved within 27 min by linear gradient. The method produced highly reproducible quantitative data at interday and intraday levels. The limits of detection were in the ng level on-column with remarkable range and linearity. The target compounds were present at mg levels in the populations, collected from inland (Mistissini and Nemaska) and costal (Waskagnish and Chisasibi) Cree communities of northern Quebec. A triterpene, 23-hydroxybetulin, was the most abundant, while betulinic acid and uvaol were minor constituents. Overall, HPLC-ELSD analyses produced very similar profiles and contents of the eight compounds in the plants collected from four geographic locations. The developed HPLC-ELSD method can be used as a targeted analysis of triterpenes in these medicinal plants.

Informal seed saving and farmer-to-farmer seed exchange are dictated by local rules and practices, which in turn can influence the distribution and management of crop biodiversity. Such systems are essential components of the livelihoods of small-holder farmers in Kenya and for in situ conservation of intraspecific and interspecific diversity. Using a mixed methods approach combining interviews, focus groups, and a household survey, we identify that the distribution of crop varieties in Tharaka, Kenya, has been shaped by both human migrations that have transported seeds and gender roles that have restricted travel and utilization of local indigenous varieties during early migrations. Our results suggest that the migrations of the first Tharaka people from Mbwa 300 years ago have influenced the concentration of local indigenous varieties in the areas of first settlement today. Subsequent smaller migrations dictated by men within Tharaka through the 1960s, particularly to the northern areas, origin...

This article examines contemporary challenges of formal seed sector participation for resource-poor farmers in Tharaka and engages in a wider discussion of national seed policy and formal seed sector development in Kenya. While many farmers reported utilising modern seed varieties developed by the formal seed sector, the majority of these were introduced through seed aid and maintained through seed saving, supporting seed system integration strategies. Building on these findings, the article discusses ways in which national seed policy in Kenya might be refined to better meet national and regional development goals focused on decreasing the incidence of hunger and poverty.

We sought to compare the abilities of the specialist Lepidoptera Pyrrhopyge thericles (Hesperiidae) and the generalist Periphoba arcaei (Saturniidae) to assimilate three highly cytotoxic compounds from their larval host plant, Vismia baccifera (Clusiaceae) and to determine whether either insect discriminated in its assimilation of the compounds that are structurally similar but of variable cytotoxicity. Vismione B (1), deacetylvismione A (2), and deacetylvismione H (3) are cytotoxic compounds isolated from V. baccifera. Compound 1 was found in the 2nd and 3rd instars of P. arcaei, but not in the mature larvae or the pupae. Pyrrhopyge thericles assimilated trace quantities of compound 1 and deacetylvismione A (2), which were both found in the 3rd and 4th instars. In extracts of V. baccifera, compound 2 is present at levels approximately 6-fold greater than compound 1, indicating that the generalist P. arcaei is capable of selectively sequestering cytotoxic compounds from its host plant. Compounds 1 and 2 show comparable cytotoxicities in three different cancer cell lines, suggesting that properties other than cytotoxicity are responsible for the selective sequestration of 1 by P. arcaei. This study represents the first time that sequestration of this class of compounds has been recorded in the Lepidoptera.

Background.Inflammation, together with related oxidative stress, is linked with the etiology of kwashiorkor, a form of severe acute malnutrition in children. A diet rich in anti-inflammatory and antioxidant phytochemicals may offer potential for the prevention and treatment of kwashiorkor. We selected and assayed five leafy green vegetables, two wild fruits, and six medicinal plants from Kenya for their antioxidant and anti-inflammatory properties. Consensus regarding medicinal plant use was established from ethnobotanical data.Methods. Antioxidant activity and phenolic content were determined using the oxygen radical absorbance capacity (ORAC) assay and Folin-Ciocalteu procedure, respectively. Anti-inflammatory activity was assessedin vitrotargeting the inflammatory mediator tumour necrosis factor-alpha (TNF-α).Results. Mangifera indica(leaves used medicinally) showed the greatest antioxidant activity (5940 ± 632 µM TE/µg) and total phenolic content (337 ± 3 mg GAE/g) butAmaranthus dubius(leafy vegetable) showed the greatest inhibition of TNF-α(IC50= 9 ± 1 μg/mL), followed byOcimum americanum(medicinal plant) (IC50= 16 ± 1 μg/mL). Informant consensus was significantly correlated with anti-inflammatory effects among active medicinal plants (r2=0.7639, P=0.0228).Conclusions. Several plant species commonly consumed by Kenyan children possess activity profiles relevant to the prevention and treatment of kwashiorkor and warrant further investigation.

Pearl millet (Pennisetum glaucum) has been maintained in Tharaka for hundreds of years and continues to be an essential staple food. Our study documented ten pearl millet varieties maintained by Tharakan farmers through seed selection and seed saving and two extinct pearl millet varieties. Farmers in Tharaka manage risk of crop failure and heterogeneous rainfall patterns by mixing early and late maturing varieties of pearl millet. Over time, however, introduced varieties that mature earlier and introduced varieties that provide more yields have replaced the older indigenous varieties. Seed selection practices focus on long panicles and big seeds as well as rogueing out off–types called mathara. There are strikingly similar parallels between mathara and the West African off–type shibra. We suggest that future studies should emphasize the process of how seed–related knowledge has shifted or can shift to accommodate challenges related to maintaining early maturing characters and to offsetting unpredictable rainfall patterns.

Pervasive food insecurity and poverty in much of the world drives vulnerable populations to harvest natural resources as a means of generating income and meeting other household needs. Wild edible plants (WEPs) are a particularly common and effective coping strategy used to increase socio-ecological resilience in Sub-Saharan Africa where agricultural systems are often sensitive to environmental perturbations and instability. WEPs are collected across the landscape, from agricultural areas to government-managed hilltops with varying degrees of success and legality. This multiple case study research, conducted in Eastern Province, Kenya, investigates the formal forest regulations and land tenure rights, as well as local enforcement and understanding of those rules, in order to understand their impact on the ability of vulnerable populations to use WEPs as a coping strategy. The results suggest that widespread confusion, trust issues and a strong focus on the commercialization of wild foods are limiting the possible contribution of WEPs to food security and increased socio-ecological resilience. We identify a number of policy changes and extension programs that could better support local communities relying on WEPs for subsistence purposes to improve their adaptive capacity.

Machakos and Makueni counties in Kenya are associated with historical land degradation, climate change, and food insecurity. Both counties lie in lower midland (LM) lower humidity to semiarid (LM4), and semiarid (LM5) agroecological zones (AEZ). We assessed food security, dietary diversity, and nutritional status of children and women.Materials and Methods.A total of 277 woman-child pairs aged 15–46 years and 6–36 months respectively, were recruited from farmer households. Food security and dietary diversity were assessed using standard tools. Weight and height, or length in children, were used for computation of nutritional status.Findings.No significant difference (P>0.05) was observed in food security and dietary diversity score (DDS) between LM4 and LM5. Stunting, wasting, and underweight levels among children in LM4 and LM5 were comparable as were BMI scores among women. However, significant associations (P=0.023) were found between severe food insecurity and nutritional status of children but not of their caregivers. Stunting was significantly higher in older children (>2 years) and among children whose caregivers were older.Conclusion. Differences in AEZ may not affect dietary diversity and nutritional status of farmer households. Consequently use of DDS may lead to underestimation of food insecurity in semiarid settings.

Evidence supports the health promoting benefits of berries, particularly with regard to the prevention and management of chronic diseases such cardio- and cerebrovascular disease, diabetes and Alzheimer’s disease. Two related pathophysiological features common to many of these conditions are oxidative stress and the accumulation of advanced glycation endproducts (AGEs). Whereas antioxidant properties are well-established in several species of berries and are believed central to their protective mechanisms, few studies have investigated the effects of berries on AGE formation. Here, employing a series of complementary in vitro assays, we evaluated a collection of wild berry extracts for 1) inhibitory effects on fluorescent-AGE and Ne- (carboxymethyl)lysine-albumin adduct formation, 2) radical scavenging activity and 3) total phenolic and anthocyanin content. All samples reduced AGE formation in a concentration-dependent manner that correlated positively with each extract’s total phenolic content and, to a lesser degree, total anthocyanin content. Inhibition of AGE formation was similarly related to radical scavenging activities. Adding antiglycation activity to the list of established functional properties ascribed to berries and their phenolic metabolites, our data provide further insight into the active compounds and protective mechanisms through which berry consumption may aid in the prevention and treatment of chronic diseases associated with AGE accumulation and toxicity. As widely available, safe and nutritious foods, berries represent a promising dietary intervention worthy of further investigation.

Background Diet and nutrition-related behaviours are embedded in cultural and environmental contexts: adoption of new knowledge depends on how easily it can be integrated into existing knowledge systems. As dietary diversity promotion becomes an increasingly common component of nutrition education, understanding local nutrition knowledge systems and local concepts about dietary diversity is essential to formulate efficient messages. Methods This paper draws on in-depth qualitative ethnographic research conducted in small-scale agricultural communities in Tanzania. Data were collected using interviews, focus group discussions and participant observation in the East Usambara Mountains, an area that is home primarily to the Shambaa and Bondei ethnic groups, but has a long history of ethnic diversity and ethnic intermixing. Results The data showed a high degree of consensus among participants who reported that dietary diversity is important because it maintains and enhances appetite across days, months and seasons. Local people reported that sufficient cash resources, agrobiodiversity, heterogeneity within the landscape, and livelihood diversity all supported their ability to consume a varied diet and achieve good nutritional status. Other variables affecting diet and dietary diversity included seasonality, household size, and gender. Conclusions The results suggest that dietary diversity was perceived as something all people, both rich and poor, could achieve. There was significant overlap between local and scientific understandings of dietary diversity, suggesting that novel information on the importance of dietary diversity promoted through education will likely be easily integrated into the existing knowledge systems. Electronic supplementary material The online version of this article (doi:10.1186/s13002-017-0150-2) contains supplementary material, which is available to authorized users.

This study explored the role of wild foods in the diets of children and mothers in the East Usambara Mountains (N = 274 dyads). We identified 92 wild food species. Although dietary diversity (most measures) was not different between seasons, wild foods accounted for a greater percentage of items consumed in the wet (food insecure) season. Many wild foods were obtained on farm; wild foods obtained from the forest accounted for less than 3% of food items consumed. Wild foods were used by virtually all informants but contributed only 2% of total energy in the diet. However, they contributed large percentages of vitamin A (RAE) (31%), vitamin C (20%), and iron (19.19%). Agricultural factors (e.g., hours spent in farm) were associated with greater wild food use. These findings suggest participation in agriculture may be important for the maintenance of wild food use, and that wild foods can play an important role in the nutritional resilience of local people.

Traditional food systems offer a key link between the social and economic resilience of smallholder farmers and pastoralists and the sustainable food and nutrition security of global populations. This paper addresses issues related to socio-cultural diversity and the continuing complex engagement of traditional and modern communities with the plants and animals that sustain them. In light of some of the unhealthful consequences of the 'nutrition transition' to globalized modern diets, the authors define and propose a process for a more successful food system transition that balances agro-biodiversity and processed commodities to support diet diversity, health and social equity alongside sustainable economic growth. We review empirical research in support of practice and policy changes in agriculture, economic development and health domains as well as cross-sectoral and community-based innovation. High-value food crops within domestic and global value chains can be an entry point for smallholders' participation as contributors and beneficiaries of development, while sustainable small farms, as purveyors of environmental and public health services, diversify global options for long-term adaptation in the face of environmental uncertainty. © 2013 Society of Chemical Industry.

SUMMARY Food insecurity and malnutrition in local populations both result from and drive deforestation. This paper examines the relationships between diet of local people and measures of forest cover and use in the East Usambara Mountains, Tanzania. Data on dietary diversity and intake were collected for 270 children and their mothers. Area of tree cover within the vicinity of each household was examined in relation to forest use and diet. Individuals using foods from forest and other non-farm land had higher dietary diversity, consumed more animal source foods and had more nutrient dense diets. They also had more tree cover in a close proximity to the home, suggesting a relationship between tree cover and forest food use. Households reporting trips to the forest had lower area of tree cover within close proximity, suggesting that land close to the home with tree cover such as agroforest and fallow is important for obtaining subsistence products. Although historically there has been little motivation for local people to participate in forest conservation in the East Usambaras, the maintenance of tree cover in the landscape around the home, especially on agricultural and village land, may be important in ensuring continued access to the health benefits potentially available in wild and forest foods.

ObjectivesWild edible plants (WEP) play the dual role of securing food diversity and promoting health in traditional societies. Current simplified dietary habits contribute to increasing population health risks. Since WEP are integral to the diet of traditional communities, identifying their significance as foods to people provides further evidence to conserve them and promote their consumption. Six species of WEP were identified as integral to the diet of rural Lebanese communities. We investigated their patterns of consumption and knowledge regarding their health and medicinal properties.DesignAn ethnobotanical survey, employing a qualitative questionnaire, was conducted among thirty informants. The identified species were Cichorium intybus, Eryngium creticum, Foeniculum vulgare, Malva sylvestris, Thymus syriacus and Gundelia tournifortii.ResultsMost informants consumed the six plants as a regular part of their diet. Seasonal variability in consumption was evident. C. intybus was renowned for its digestive and blood strengthening properties. F. vulgare was used as a digestive stimulant. M. sylvestris was distinguished for its anti-inflammatory qualities. T. syriacus was popular for its digestive and anti-poisonous properties. E. creticum was attributed less pronounced health benefits. G. tournifortii was considered a nutritious food.ConclusionsAll six species were popular for their edible uses as well as their health and/or medicinal benefits. These properties are supported by scientific evidence. Our results highlight the importance of these plants for local people and support efforts for their conservation. However, we noticed a decline in indigenous knowledge. We encourage efforts to record it for other plants and in other communities.

We examined Coleoptera and Lepidoptera assemblages feeding on two different groups of plants: one in which plants were active against cancer cell lines and/or protozoan parasites responsible for tropical parasitic diseases, and a second group that was inactive in the same bioassays. Aposematic species were found on nine of the ten active plant species, but on only four of the ten inactive plant species. Non-aposematic insects did not show a significant difference in their association with active versus inactive plants. Our results suggest that the presence of aposematic, herbivorous insects can be used to facilitate the identification of plants with compounds active against important human diseases.

Traditional medicines (TM) used to treat symptoms of diabetes by two Cree communities were assessed for their free radical scavenging activity using the stable 1,1-diphenyl-2-picrylhydrazyl radical, and their ability to protect human low-density lipoprotein from Cu2+-mediated oxidation by measuring lag time before the appearance of conjugated dienes and formation of thiobarbituric acid-reactive substances. Water-soluble phenolic content was also measured. A total of 20 medicinal plants from Whapmagoostui and 16 from Mistissini were compared with 16 extracts of plants that were not used medicinally. Medicinal plant extracts, particularly those from Larix laricina, displayed high antioxidant activity, comparable with ascorbic acid, Trolox, and the known antioxidant flavonoids quercetin, epicatechin, catechin. Extracts of Pinaceae and Ericaceae contained the highest levels of phenolics. Factors such as season and area of plant collection, as well as the plant part from which the extract was derived, affected antioxidant activity. Positive correlations were found between the established traditional knowledge of Cree Elders and Healers and the antioxidant activity for medicinal plants used in Mistissini (r = 0.3134; p = 0.058) and in Whapmagoostui (r = 0.5165; p = 0.001). Significant correlations between phenolic content were also seen with the existing ethnobotanical data (r = 0.5015; p = 0.003) and bioassays (r = 0.4178; p = 0.003). These results indicate that a clear majority of plants used by the Cree are excellent sources of antioxidants.

Although ethnopharmacological investigations emphasize the importance of medicinal plants in developing countries, species used regularly with diet are under-investigated and potentially make greater contributions to health. Thirteen traditional plants most commonly added to milk/soups by the Maasai for perceived health benefits were tested for activity against measles virus (MV) using non-medicinal plants as controls. Antiviral effects of plant extracts were sought using a modified neutralization assay. Methanolic extracts of medicinal species exhibited significantly greater activity neutralizing MV in vitro in comparison to non-medicinal extracts (p

To examine the effects of Momordica charantia on glucose uptake and adiponectin secretion in adipose cells, 3T3-L1 adipocytes were treated with three concentrations (0.2, 0.3 and 0.4mg/ml) of water and ethanol extracts of Momordica charantia fruit and seeds alone and in combination with either 0.5nM or 50nM insulin. The treatment combination of 0.2mg/ml water extract and 0.5nM insulin was associated with significant (p

Biofortification, the focus of the HarvestPlus program of the Consultative Group on International Agriculture Research (CGIAR), represents a potentially powerful tool to increase dietary intake of essential nutrients in staple foods. This paper evaluates the compatibility of biofortification with the preferred option of dietary diversification and its potential impacts on the agricultural biodiversity essential for long term sustainability. In poor countries, biofortification requires increasing public investment in agricultural research and infrastructure for success. Rather than cereal commodities, biofortification for developing countries should focus on vegetatively propagated species or in improving quality of coarse cereals, as well as fodders. Community participatory approaches that identify local food resources with nutritional, agronomic and economic advantages to small-scale farmers could complement and set targets for biofortification as one of many approaches to alleviate nutritional deficiencies. Furthermore using agricultural biodiversity to reinforce dietary diversity can help situate biofortification within the larger context of sustainable food-based approaches. In this light, this paper evaluates specific biofortification interventions from environmental, sociocultural, political, economic, ethical, and biomedical perspectives.

Studies of land-use/land cover change are an important means for examining the viability of community-based programs for forest conservation, although an analysis of the social processes influencing land-use decisions is necessary to understand the factors leading to different conservation outcomes. In this paper, we demonstrate that an analysis of locally recognized land-use rules and regulations embedded in local institutions can inform remote-sensing approaches by helping: (1) to elucidate some of the local perceptions, criteria and interactions with outside agencies that drive conservation actions and (2) to better interpret the spatial patterns of land-use change and forest conservation revealed by remote-sensing data. Based on a case study of a forest ejido from the Maya Zone of Quintana Roo, Mexico, we evaluate changes in forest cover and in local land-use regulations before and after the initiation in the mid-1980s of a community forestry program, the Plan Piloto Forestal (PPF). Methods included development of a time series of land cover maps based on LANDSAT imagery from 1976, 1988, 1991, 1997 and 2000, as well as interviews and participant observation with local farmers and community leaders. Results indicate a high degree of forest conservation on community lands with net rates of forest loss of 0.6‐0.7%/year. Locally recognized conservation regulations included a number of forest reserves as well as regulations which protect specific forest types, resulting from both local initiatives and interventions of external agencies. These initiatives included but were not limited to the PPF, highlighting the importance of evaluating community-based conservation programs within a broader historical context. Conservation regulations protecting an important commercial non-timber forest product (Manilkara zapota) pre-dated the PPF and may have facilitated its implementation. In the most accessible agricultural areas, the only mature forest patches were customary forest reserves and an area regenerated from secondary forests, protected due to enrichment plantings of commercial timbers. Recognition of local Maya terminology used to distinguish forest types was crucial for proper interpretation of local land-use regulations, which revealed that less-valued forest types may not be adequately protected on community lands. We suggest that future research should examine the significance of the less-valued forest types for global biodiversity conservation. In addition careful consideration of the historical antecedents and community institutions which may have facilitated the implementation of the Plan Piloto Forestal will be important for the successful application of this model of community forestry to other socio-economic and cultural contexts. # 2005 Elsevier B.V. All rights reserved.

"Wild edible plants (WEPs) are known to make important contributions to food baskets and livelihoods in the smallholder and subsistence farming communities of sub-Saharan Africa. As a result, protecting and promoting the sustainable use of WEPs in concert with more mainstream agricultural innovation efforts has the potential to build household resilience to food insecurity. There is, however, a need to better understand how WEPs contribute to rural livelihoods on a daily basis and act as emergency safety nets during periods of hunger. Focusing on two villages in rural eastern Kenya, we sought to determine which household conditions are correlated with household reliance on WEPs as a coping strategy during times of food insecurity, while also investigating the role of access restrictions on adaptive capacity and the ability to obtain these important food resources. Results reveal that reliance on WEPs is greater in households that report food insecurity, lack off-farm income, and have lower asset levels. Access to WEPs is also a major factor in consumption frequency, with smaller farm sizes and increased distance to harvest areas significantly correlated with lower levels of WEP use. By combining vulnerability and adaptive capacity measures for each household, we created a more complete accounting of the factors that influence WEP consumption frequency, with implications for policy. This study represents an important first step in taking a more holistic view of the subsistence value of WEPs and the myriad factors that influence households’ reliance on, and ability to obtain, uncultivated natural resources."

More than 200 medical practitioners and clinical researchers in 82 clinical care and research centers in Kenya, Tanzania, and Uganda responded to a survey on the status of clinical research in East Africa. The results showed that: (1) human capacity for clinical research exists but needs strengthening; (2) research is hampered by nonexistent or poor facilities and by unclear regulations and ethical procedures; (3) medical practitioners in most remote sites are overwhelmed with large numbers of patients and are isolated from research activities; and (4) above all, lack of political will, funding, and sponsor interest are major obstacles to clinical research. Recommended priorities for preparing the region for clinical trials should be: regional harmonization of regulatory affairs, ethical procedures, and a Good Clinical Practice guideline; public education; and community outreach programs. Lastly, capacity building efforts in clinical monitoring, research site coordination, data management, and biostatistics should be undertaken immediately in order to meet the demand over the next decade.

An agricultural project in Highland Ecuador provided a model context to better understand the nutrition of rural women. The adequacy of women's nutrition and the strength of associations with age and socioeconomic status were studied in 104 rural households over four rounds (two seasons) during the 1995–1996 agricultural year using a cross-sectional with repeated-measures design. Women were at high risk for micronutrient deficiencies (calcium, iron, riboflavin, and vitamin B12) due to low intakes of animal products. Two distinct constructs representing socioeconomic status were identified: modern lifestyle and farming wealth. In multivariate models, farming wealth was associated with quality of women's diet (animal protein adjusted for energy, p = 0.01). Diet quality, in turn, was positively associated with anthropometric status (p = 0.02). Women over the age of 50 weighed approximately 3.7 kg less than younger women and consumed less energy (300 kcal) and micronutrients (p < 0.05). Age was positively associated with respiratory morbidity (p = 0.01). These findings, while directly relevant to a specific context, suggest the need for cross-cultural studies to identify the extent of, and factors contributing to, the risk of nutritional inadequacy in postreproductive women in developing countries.