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Background Cold snare polypectomy (CSP) is the standard of care for the resection of small ( Methods A prospective multicenter observational study was conducted of all morphologically suitable nonpedunculated colonic polyps of 10–19 mm removed by CSP/C-EMR between May 2018 and June 2021. Once resection was complete, multiple biopsies were taken of the margins circumferentially and centrally. The primary outcome was the incomplete resection rate (IRR), based on residual polyp in these biopsy specimens. Secondary outcomes were recurrence rate at first surveillance colonoscopy and rates of adverse events (AEs). Results CSP/C-EMR was performed for 350 polyps (median size 15 mm; 266 [76.0 %] Paris 0-IIa classification) in 295 patients. Submucosal injection was used for 87.1 % (n = 305) of polyps. Histology showed 68.6 % adenomas, 26.0 % sessile serrated lesions (SSLs) without dysplasia, 4.0 % SSL with dysplasia, and 1.4 % hyperplastic polyps. The IRRs based on margin or central biopsies being positive were 1.7 % (n = 6) and 0.3 % (n = 1), respectively. The polyp recurrence rate was 1.7 % (n = 4) at first surveillance colonoscopy – completed for 65.4 % (n = 229) of polyps at a median interval of 9.7 months. AEs occurred in 3.4 % (n = 10) of patients: four with post-polypectomy pain; three self-limiting post-polypectomy bleeds; two post-polypectomy-syndrome-like presentations; and one intraprocedural bleed treated with clips. There were no perforations. Conclusion CSP/C-EMR for morphologically suitable nonpedunculated colonic polyps of 10–19 mm is effective and safe, including for conventional adenomas. Rates of incomplete resection and recurrence were low, with few AEs. Studies directly comparing this method with hot snare resection are required.

To investigate methods for in vitro assessment of anthelmintic efficacy against the chicken nematode Ascaridia galli this study firstly evaluated sample preparation methods including recovery of eggs from excreta using different flotation fluids and induced larval hatching by the deshelling-centrifugation method and the glass-bead method with or without bile. It then evaluated two in vitro assays, the in-ovo larval development assay (LDA) and larval migration inhibition assay (LMIA), for anthelmintic efficacy testing against A. galli using fresh eggs and artificially hatched larvae, respectively. Four anthelmintics, thiabendazole (TBZ), fenbendazole (FBZ), levamisole (LEV) and piperazine (PIP) were employed using an A. galli isolate of known susceptibility. The results suggested that the LDA and LMIA could successfully be used to generate concentration response curves for the tested drugs. The LDA provided EC50 values for inhibition of egg embryonation of 0.084 and 0.071 μg/ml for TBZ and FBZ, respectively. In the LMIA, the values of effective concentration (EC50) of TBZ, FBZ, LEV and PIP were 105.9, 6.32, 349.9 and 6.78 × 107 nM, respectively. For such in vitro studies, a saturated sugar solution showed high egg recovery efficiency (67.8%) and yielded eggs of the highest morphological quality (98.1%) and subsequent developmental ability (93.3%). The larval hatching assays evaluated did not differ in hatching efficiency but the deshelling-centrifugation method yielded larvae that had slightly better survival rates. For final standardization of these tests and establishment of EC50 reference values, tests using isolates of A. galli of defined resistance status need to be performed.

To investigate methods for in vitro assessment of anthelmintic efficacy against the chicken nematode Ascaridia galli this study firstly evaluated sample preparation methods including recovery of eggs from excreta using different flotation fluids and induced larval hatching by the deshelling–centrifugation method and the glass-bead method with or without bile. It then evaluated two in vitro assays, the in-ovo larval development assay (LDA) and larval migration inhibition assay (LMIA), for anthelmintic efficacy testing against A. galli using fresh eggs and artificially hatched larvae, respectively. Four anthelmintics, thiabendazole (TBZ), fenbendazole (FBZ), levamisole (LEV) and piperazine (PIP) were employed using an A. galli isolate of known susceptibility. The results suggested that the LDA and LMIA could successfully be used to generate concentration response curves for the tested drugs. The LDA provided EC50 values for inhibition of egg embryonation of 0.084 and 0.071 μg/ml for TBZ and FBZ, respectively. In the LMIA, the values of effective concentration (EC50) of TBZ, FBZ, LEV and PIP were 105.9, 6.32, 349.9 and 6.78 × 107 nM, respectively. For such in vitro studies, a saturated sugar solution showed high egg recovery efficiency (67.8%) and yielded eggs of the highest morphological quality (98.1%) and subsequent developmental ability (93.3%). The larval hatching assays evaluated did not differ in hatching efficiency but the deshelling–centrifugation method yielded larvae that had slightly better survival rates. For final standardization of these tests and establishment of EC50 reference values, tests using isolates of A. galli of defined resistance status need to be performed.

The efficacy of commercially available anthelmintics against mature and immature stages (including ovicidal effects) of two Australian field isolates of Ascaridia galli was evaluated in two separate experiments. The anthelmintics tested were levamisole (LEV), piperazine (PIP) and flubendazole (FBZ) plus LEV-PIP. A total of 192 artificially trickle-infected young cockerels (96 birds per isolate) were randomized into sixteen experimental groups of 12 cockerels each (7 treatments and 1 untreated control per isolate). Chickens received label-recommended doses of LEV (28 mg/kg), PIP (100 mg/kg) or LEV-PIP co-administered at their full individual doses as a single oral dose or in group drinking water at recommended concentrations of 0.8 mg/ml or 2.5 mg/ml over eight hours for 1 and 2 days respectively and FLBZ (30 ppm) in the feed over 7 days. Anthelmintic efficacies were assessed by worm count reduction (WCR%) and excreta egg count reduction (EECR%) estimated by two methods. Ten days post treatment, all untreated control birds harboured mixed worm population of 10.1 and 12.3/bird for each isolate respectively which was significantly higher (P0.0001) than counts in all treatment groups. Luminal or histotrophic larvae comprised 50-57 % of the total worm count. For LEV, PIP and LEV-PIP, individual oral administration provided a somewhat higher efficacy than group medication in drinking water. EECR% values were inconsistent with WCR% and found to be only an indicator of efficacy against adult worms. All developmental stages of the two A. galli isolates were highly susceptible to FLBZ (100 %) followed by LEV-PIP (92.4-100 %) and LEV (87.7-100 %). PIP exhibited good efficacy against adult worms (92-97 %) but reduced efficacy against luminal (79-84 %) and histotrophic (61-72 %) larvae. Embryonation capacity of eggs recovered from worms expelled after treatment with LEV (47-54 %), PIP (44-54 %) or LEV-PIP (45-48 %) did not differ from those from untreated birds (50-51 %) whereas eggs from FLBZ treated worms had a significantly lower (P0.05) capacity to embryonate (≤ 2 %). Put together, our results demonstrate no evidence of resistance of the test A. galli isolates to the tested anthelmintics but a significant advantage of FLBZ, followed by LEV-PIP and LEV over PIP in the control of A. galli, specifically with regard to immature stages. A. galli worms expelled after treatment with LEV, PIP or their combination, but not FLBZ contain viable eggs. This has epidemiological implications and may also provide an option for isolating eggs from mature worms for A. galli propagation experiments without having to sacrifice birds.

Ascaridia galli infection models use eggs isolated from chicken excreta, worm uteri and worms cultured in artificial media. The aim of this study was to compare the infectivity of A. galli eggs isolated from these sources under two infection regimens. A 3 × 2 factorial arrangement was employed to test the infectivity of A. galli eggs from the three sources and two modes of infection (single or trickle infection). One hundred and fifty-six Isa-Brown one day-old cockerels randomly assigned to the six treatment groups (n = 26) were orally infected with embryonated A. galli eggs obtained from the three A. galli egg sources (worm uteri, excreta or eggs shed in vitro) administered either as single dose of 300 eggs at one day-old or trickle infected with 3 doses of 100 eggs over the first week of life. Twenty-two negative control birds remained uninfected. Eggs obtained from cultured worms or excreta exhibited a higher embryonation capacity (P = 0.003) than eggs obtained from worm uteri. There were higher worm establishment (infectivity) rates from embryonated eggs originating from cultured worms and worm uteri compared with eggs obtained from fresh excreta (P 0.0001). Trickle infection resulted in a significantly higher total worm burden (P = 0.002), establishment rate (P = 0.002) and excreta egg counts (EEC, P = 0.025) than single infection. Worm length was greater in birds infected with embryonated eggs from excreta than from uteri or cultured worms (P 0.0001). However, mode of infection did not affect worm length (P = 0.719) and weight (P = 0.945). A strong significant positive linear correlation was observed between EECs and female worm counts at 12 weeks of post infection sampling (r = 0.75; P 0.0001). Body weight of birds was negatively correlated with both worm burden (r = - 0.21; P 0.01) and EEC (r = - 0.20; P 0.05) at 12 weeks post infection. In conclusion, our results show that eggs shed by cultured worms or isolated from worm uteri had greater infective capacity than eggs harvested from excreta and that trickle rather than bolus infection resulted in higher worm establishment. These factors should be taken into account when considering artificial infection protocols for A. galli.

With the continued growth of free-range egg production, the importance of the chicken roundworm Ascaridia galli is increasing. Investigations into this parasite would be facilitated by the availability of characterised strains and clear guidelines on optimal methods of multiplication and maintenance. Currently, there is lack of well-defined in vivo models for maintaining A. galli and the potential of using host immunosuppression to boost parasite development and worm egg output has not been investigated. To determine the most efficient way of propagating A. galli in young chickens an experiment with a 2 × 3 × 4 × 2 factorial design involving age of chicken at infection (day-old or 14 days old), immunosuppression (dexamethasone (DEX), cyclophosphamide (CY) or sham), infective egg dose (0, 100, 300 or 900 embryonated eggs/bird) and time of worm recovery after infection (8 or 10 weeks post-infection) was conducted. The experiment used a total of 384 layer cockerel chicks. Infection was delivered orally in 3 split doses over one week and immunosuppressants were administered by intramuscular injection concurrently with the infections. Body weight, excreta egg counts, intestinal worm count and worm establishment rate were assessed. The only sign of ascaridiosis noted was mild diarrhoea at the time of slaughter in some birds with a significant positive association with worm count. Infection caused a significant dose dependent reduction in body weight in non-immunosuppressed birds but this effect was ameliorated by immunosuppression. Age at infection had no significant effect on the studied variables although both worm and egg counts were numerically higher in the day-old infected groups. Egg dose significantly influenced the prevalence of infection, worm establishment rate, worm egg production and mean worm count. The 300 and 900 egg doses resulted in significantly higher worm count and egg production than the 100 egg dose. A significant negative correlation was observed between egg dose and worm establishment rate indicating an inverse relationship. Immunosuppression with DEX, but not CY resulted in significantly higher mean worm burden than in control chickens with excreta egg counts also considerably higher in DEX treated birds. Our results suggest that trickle infection at day-old with infective doses of 300 eggs coupled with immunosuppression with DEX would provide the most efficient way to propagate A. galli worms in vivo, as using older birds or a higher egg dose did not provide any advantage.

Eggs in the infective stage of the chicken nematode Ascaridia galli are often required for in vivo and in vitro studies on this parasite. The reliability of any artificial A. galli infection depends on the viability and embryonation capacity of A. galli eggs. The aim of this study was to determine ideal storage conditions for maximising the viability of A. galli eggs and maintaining viability for the longest period. A 2 × 2 × 3 × 5 factorial experimental design was employed to investigate the effects of storage temperature (4°C or 26°C), storage condition (aerobic or anaerobic), storage medium (water, 0.1 N H

BACKGROUND While spinal cord injury (SCI) caregiving can be a rewarding experience, caregivers often experience reduced mental and physical health. OBJECTIVE This article describes the methodology of a study examining the efficacy of a newly developed telehealth Transition Assistance Program (TAP) for caregivers of individuals with acute SCI. METHODS A mixed-methods, randomized controlled trial is comparing TAP outcomes to that of a standard-of-care control. The study is recruiting for 48 months and incorporating both quantitative and qualitative outcome measures. Data are collected regarding barriers and facilitators to using the TAP, which might inform dissemination and implementation. RESULTS This study was funded by the Craig H. Neilsen Foundation in April 2017. It was approved by the institutional review boards at Virginia Commonwealth University and the Hunter Holmes McGuire Veterans Affairs Medical Center that same year. Participant recruitment and data collection began in 2018. CONCLUSIONS This study is implementing and testing an SCI caregiver intervention unlike any created before, targeting a critical time period that, until now, other SCI caregiver interventions have overlooked. Research personnel intend to disseminate the intervention and study findings through the publication of manuscripts and presentations at conferences. If the current study shows improvements in caregiver or patient well-being, the TAP for SCI caregivers could become part of the standard of care for acute SCI. CLINICALTRIAL ClinicalTrials.gov NCT03244098; https://www.clinicaltrials.gov/ct2/show/NCT03244098 INTERNATIONAL REGISTERED REPORT DERR1-10.2196/28256

Background Providing care for someone with a disease or chronic condition can have a negative psychological, physical, social, and economic impact upon informal caregivers. Despite the socio-economic relevance and more than three decades of caregiver intervention research only very few translational efforts of successful interventions are reported. Still less of these interventions have been implemented into routine services. The aim of the ReDiCare study (German acronym BerTA) is to evaluate the effectiveness of a stepped counselling approach for burdened caregivers delivered by care counsellors of two long-term care insurances and registered psychotherapists. Methods/ design A pragmatic randomised controlled trial with 572 caregivers of older adults (≥ 60 years) receiving benefits of one of the two participating long-term care insurances. Participants are assigned (t0) to either the ReDiCare intervention or a control group receiving routine care and counselling. Data are collected at baseline (-t1), 3-month (t1), 9-month (t2) and 15-month (t3). The 9-month post-intervention assessment (t2) is the primary endpoint to evaluate the results on the primary and secondary outcomes, measured by self-reported questionnaires. Depressive symptoms measured with the CES-D are the primary outcome. The main secondary outcomes are physical complaints, utilization of psychosocial resources, caregiver self-efficacy and burden, positive aspects of caregiving and perceived care quality. A process evaluation, including audio tapes, self-report questionnaires and documentation will be conducted to examine internal and external validity of the intervention. Data on direct and indirect costs are collected for the (health) economic evaluation, using a health care perspective and a societal perspective. Discussion While comparable previous caregiver interventions have been developed and evaluated for specific caregiver groups (e.g. dementia caregivers, stroke caregivers), the ReDiCare study will indicate whether a stepped approach will be effective also in a broader group of caregivers. The intervention is one of the very few translational studies in caregiver intervention research and will provide valuable insights into relevant factors for training, intervention protocol adherence, effectiveness, and costs for future implementation steps. Trial registration Deutsches Register Klinischer Studien (German Clinical Trials Register), DRKS00014593 (www.drks.de, registered 14 May 2018) and International Clinical Trials Registry Platform, DRKS00014593 (https://apps.who.int/trialsearch/).

This study investigated worm control practices by free-range egg farmers and the efficacy of the commercial anthelmintics levamisole (LEV), piperazine (PIP), flubendazole (FLBZ) and fenbendazole (FBZ) against gastrointestinal nematodes on two free-range layer farms in Australia. An online survey comprising 36 questions was designed and implemented using SurveyMonkey. The survey contained questions about participant demographics, farm and flock characteristics, perceived intestinal worm importance, infection monitoring, deworming and other worm control practices. A link for the survey was emailed to free range egg producers from their industry body in December 2019. The anthelmintic efficacy trial was conducted in a total of 229 layers naturally infected with Ascaridia galli, Hetarakis gallinarum, Capillaria spp. and/or tape worms. Chickens received a single oral dose of LEV (28 mg/kg), PIP (100 mg/kg), FBZ (10 mg/kg) or LEV-PIP co-administered at their full individual doses, and FLBZ (Flubenol®), 30 ppm or 60 ppm) in the feed over 7 days. Anthelmintic efficacies were estimated by both worm count reduction (WCR %) and excreta egg count reduction (EECR %) tests 10 days after start of treatment. The survey with a response rate of 16/203, revealed that worm infection was of moderate concern to the producers and the majority (68%) felt that the current anthelmintics work effectively. The level of understanding of worms, monitoring and control practices did not reveal any major deficiencies of concern. The most commonly used anthelmintic was LEV (73%) followed by PIP (45%). Based on a standard cut-off value (≥90%), LEV, LEV-PIP, and FBZ attained the desired efficacy but PIP exhibited reduced efficacy against immature A. galli (61-85%), all stages of H. gallinarum (42-77%) and Capillaria spp. (25-44%). FLBZ was highly effective against all stages of roundworms and tapeworm infections. Even though there was some association between the efficacies estimated by WCR % and EECR % the latter was poorly associated in the natural infection model and hence does not provide a reasonable alternative for assessing anthelmintic efficacy when immature stages of the lifecycle are included. These results show no evidence of loss of susceptibility to the tested anthelmintics on these farms supporting the perception of producers that participated in the survey that current treatments work effectively. The reduced efficacy of PIP against some species and immature stages is related to its spectrum of activity rather than providing evidence of emerging resistance.

Gastrointestinal helminth parasites are a concern for the poultry industry worldwide as they can affect the health, welfare, and production performance. A systematic review of the prevalence over time in different countries may improve our understanding of gastrointestinal helminthiasis in chickens and subsequently lead to improved poultry health. The aim of this systematic review and meta-analysis was to provide an overview of the published information regarding the epidemiology and the diagnostic approaches of chicken helminth infection. Six databases were searched for studies, and a total of 2,985 articles published between 1942 and 2019 were identified and subsequently screened for eligibility using title or abstract and full text assessment, resulting in 191 publications to be used in the study. Postmortem diagnostics (73.8%) and the flotation technique (28.8%) were commonly used to detect helminth infections with a pooled prevalence of 79.4% ranging from 4 to 100%. More than 30 helminth species in chicken populations were identified including Ascaridia galli (35.9%), Heterakis gallinarum (28.5%), Capillaria spp. (5.90%), and Raillietina spp. (19.0%) being the most prevalent. The reported prevalence of helminth infection decreased over time in developing countries while it increased in the developed world. Chicken kept in backyard and free-range systems had a markedly higher pooled prevalence of helminth infection (82.6 and 84.8%, respectively) than those housed in cage production systems (63.6%). This may indicate the need for more rigorous control and prevention measures in free-range and backyard production systems using regular deworming coupled with access to early and accurate diagnosis allowing for early intervention.

Excreta egg counting techniques are used for indirectly estimating the magnitude of gastrointestinal nematode infection in live animals. The aim of this study was to optimise laboratory and field sampling methods for routine monitoring of nematode infections in chickens by evaluating the sensitivity, accuracy, and precision of the Modified McMaster (MM) and Mini-FLOTAC (MF) methods using laying chicken excreta samples spiked with estimated true numbers of eggs (Experiment 1 = 5-1500 EPG (eggs/g); Experiment 2 = 5-500 EPG) without and with operator effects, respectively or using individual fresh excreta (n = 230) and fresh floor excreta (n = 42) from naturally infected free-range layer farms. The Coefficient of Variation (CV) was assessed within and between operators and the time spent on sample preparation and counting was also evaluated. MF was more sensitive than MM at ≤ 50 EPG level but not above this while MM had a significantly higher egg recovery rate than MF for ≥ 50 EPG levels (MM = 89.7 %, MF = 68.2 %; P 0.0001). Operator factors did not have a significant effect (P = 0.358-0.998) on egg counts across methods and EPG levels. The CV between replicates of the MM and MF methods for ≥ 50 EPG was 43.4 and 36.5 %, respectively. The inter-observer CV of the MM and MF methods for ≥ 50 EPG levels was 63.8 and 44.3 % respectively. When the naturally infected free-range layers which were individual caged for excreta sampling, the proportion of samples positive for MM and MF were 91.7 and 96.5 %, respectively (P = 0.023). MM resulted in significantly (P = 0.029) higher excreta egg counts (604) than MF (460) with the difference between methods greatest at higher EPG levels. Fresh floor excreta (pooled or individual) and individual caged chicken excreta did not have significant effect on egg counts (P = 0.274). The total time taken for sample preparation and egg counting was significantly lower using the MM method (4.3-5.7 min) than the MF method (16.9-23.8 min) (P 0.0001). In conclusion, MM was more accurate than MF, particularly at higher EPG levels, but slightly less precise and sensitive, particularly at low EPG levels, while taking less than 25 % of the laboratory time per sample. Our observations indicate that the MM method is more appropriate for rapid diagnosis of chicken nematodes in the field. Pooled fresh floor excreta samples would be sufficient to indicate infection level in free range farms.

Evidence on the current efficacy status of anthelmintics used in the Australian poultry sector is lacking. A controlled trial was conducted to evaluate the efficacy of three commonly used anthelmintics, namely levamisole (LEV), piperazine (PIP) and fenbendazole (FBZ) plus levamisole-piperazine combination (LEV-PIP) against a field strain of A. galli recovered following flock treatment with LEV. A total of 108 A. galli infected cockerels were randomized into nine experimental groups of 12 cockerels each (eight treatments and one untreated control) with each treatment administered by two routes (oral drench or in drinking water). Chickens received label-recommended doses of LEV (28 mg/kg) and PIP (100 mg/kg) while LEV-PIP involved both compounds co-administered at their full individual dose rates. FBZ was tested at two dose rates; 10 mg/kg as a single oral drench or 5 mg/kg in drinking water over 5 days. Anthelmintic efficacies were assessed by worm count reduction (WCR%) and excreta egg count reduction (EECR%) estimated by two methods. Ten days post treatment, the untreated control birds harboured significantly higher worm counts (P < 0.0001) than those in all treatment groups irrespective of the mode drug of application. Oral drenching caused a greater reduction in worm and egg counts (P < 0.05) than medication in drinking water. Based on geometric worm counts the percentage efficacies for the oral drench were 99.1, 96.3, 97.2 and 100 % respectively for LEV, PIP, FBZ and LEV-PIP, and for administration in water 96.4, 93.7, 88.7 and 97.7 % respectively. Efficacies based on EECR% were consistent with WCR% with strong positive linear association between efficacy values. In conclusion, our results demonstrate no evidence of loss of susceptiblity of the test A. galli isolate to both LEV and PIP contrary to our hypothesis. Additional efficacy studies are needed using A. galli isolates sourced from different poultry flocks across Australia.

A detailed study of a 34-year aged dense non-aqueous phase liquid (DNAPL) release (51,300 L of parachlorobenzotrifluoride (commonly referred to as 4-CBTF)) was conducted in a surficial clayey aquitard in a physiographic region of southwestern Ontario known as the St. Clair Clay Plain. Prior to this study, little was known about the environmental fate of 4-CBTF. This study offered a unique opportunity to characterize and evaluate source evolution of the aged 4-CBTF DNAPL where the release conditions are well documented (known timing and contaminant volume, reasonable estimates of contaminant mass removal during preliminary remediation, etc.). Evaluation of 4-CBTF DNAPL fate was informed by insights obtained from previous studies conducted within the regional aquitard, including hydrogeologic conditions, transport properties, and DNAPL behaviour (as characterized during historic experimental releases of other DNAPL compounds). With a low solubility and high octanol water partitioning coefficient, 4-CBTF DNAPL persistence remains evident over three decades post-release, and plume mobility is strongly controlled by diffusion from fractures into the matrix in the shallow active flow zone and underlying unfractured zones. Natural Sciences and Engineering Research Council of Canada Engage Grant, Canadian National Railway Company, Golder Associates Ltd.

This paper argues that the emendation ὑπερερῶν for ms. ὑπερoρῶν in Them. 2. 3, although rejected by many editors and commentators, should be accepted. The manuscripts have Themistokles ‘despising’ practical studies, that is studies which promoted ‘intelligence and action’. But this makes little sense in context and disrupts the logic of the whole chapter, which presupposes a contrast between real education, which Themistokles rejects, and practical activities, on which he concentrates and for which he was suited by nature. It is much more plausible that Themistokles is presented as having ‘an excessive love’ for practical studies. Indeed, this combination – rejection of true education and enthusiasm for practical training – characterises several other subjects of Plutarch, while erotic metaphors are used to describe Themistokles’ obsession with practical success in the next chapter.

This paper examines Plutarch's treatment of education in theParallel Lives. Beginning with a close reading ofThem. 2, it identifies two distinct ways in which Plutarch exploits the education of his subjects: in the first, a subject's attitude to education is used to illustrate a character presented as basically static (a ‘static/illustrative’ model); in the second, a subject's education is looked at in order to explain his adult character, and education is assumed to affect character (a ‘developmental’ model). These two models are often associated with two different forms of discourse: anecdotal for the static/illustrative model and analytical for the developmental. The developmental model, furthermore, is closer to Plutarch's thinking in theoretical discussions of character in theMoralia; the static/illustrative model to Plutarch's treatment of character in theLivesmore generally, where anecdotal treatments predominate. The coexistence of these two models is probably to be seen as the result of a tension between Plutarch's philosophical thinking and his biographical practice: those few passages in theLiveswhich assume a developmental model occur in contexts where either Platonic texts or the activity of philosophers are being discussed.

Almost four decades ago, Donald Russell published in this journal an analysis of the first sixteen chapters of theLife of Alkibiades, which consist largely of short self-contained anecdotes about Alkibiades' childhood, youth and early career (Russell 1966b). As Russell demonstrated, most of these anecdotes are juxtaposed without any causal link. Although there are the occasional chronological markers – indications, for example, that Alkibiades is getting older and passing from childhood to early manhood – some are plainly out of chronological order and it is impossible to extract a clear chronology from them. Russell argued, however, that to try to extract such a chronological narrative would be to misunderstand the function of this material, which is not to provide a narrative of Alkibiades' early years but rather to illuminate and illustrate his character.Russell's argument, in particular the stress on Plutarch's interest in character, was seminal; together with two other papers published at roughly the same time, it marked the beginning of a new appreciation of Plutarch as an author of literary merit. But Russell was rather less convinced of the logic of selection of the first five anecdotes, which relate to Alkibiades' youth and comprise some one-and-a-half pages of Teubner text (Alk.2–3).

This study focuses not on individual Lives or pairs of Lives, but on the book as a whole and its articulation across the full corpus. It argues that the Plutarchan book consists of up to four distinct sections: prologue, first Life, second Life, synkrisis. Each of these sections has a fairly consistent internal structure, and each has a distinct set of strategies for opening, for closure, and for managing the transition from one section to the next. Prologues provide an introduction to both Lives, and are clearly delineated from them, even though in our manuscripts they appear as part of the first Life; in fact, there is often a stronger break between prologue and first Life than there is between the two Lives themselves. Prologues usually begin with generalized reflections, to be followed only later by the naming of the subjects and a statement of their similarities. Most Lives begin with a thematically organized section (the ‘proemial opening’), which surveys the subject's life as a whole, not just their youth, and which is marked off with varying degrees of distinctness from the narrative that follows. Crucially, proemial openings do not narrate and the logic of their structure is not chronological. Closure in many Lives is signalled by ‘circularity’ and sometimes by a closural or transitional phrase, though first Lives are different here from second Lives. Synkriseis are structured both by a series of themes on which the two subjects are compared, and by a two-part, agonistic structure in which first one of the subjects is preferred, then the other. Synkriseis may also recall the prologue; both prologue and synkrisis operate at the level of the book, and between them frame and weld together the two Lives.

Plutarch begins the biography by selecting incidents and an epigram that anticipate the basic themes of Themistocles' life--his greatness, sometimes ambiguous, and his benefactions to all of Greece.