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BACKGROUND AND PURPOSE Radon and its radioactive progenies are the most important source of ionizing radiation of natural origin, being classified as a Group 1 carcinogen. The aim of this study is to investigate the genotoxic effects of a wide range of indoor radon concentrations, as well as the kinetics of the process of repairing DNA-induced lesions by a challenging dose of gamma irradiation. MATERIAL AND METHODS Female subjects residing in the Baiţa-Stei radon priority area were selected as the exposed group. The reference group was comprised of women from the same county (Bihor), but located in an area with an average indoor radon concentration typical of the county from which they were taken. Radon concentration values of 300 Bq/m3 and 148 Bq/m3, respectively, were chosen as a threshold in order to capture the impact of radon exposure between the groups. The alkaline comet assay was used in order to measure the DNA damage, as well as the repair kinetics at 2 and 24 h after 2 Gy challenging doses of gamma irradiation using peripheral blood lymphocytes. From the serum of the subjects, the oxidative damage by 8-hydroxydeoxyguanosine as well as the PARP induction was evaluated. The chromosomal aberrations were evaluated using the Cytokinesis Block MicroNucleus Assay. RESULTS A statistically significant increase was observed in terms of DNA-induced lesions assessed by comet assay for the exposed group compared to the reference group. A positive correlation was obtained between DNA damage and the annual effective dose, respectively with the radon progenies concentrations. A statistically significant difference was also observed for the frequency of the micronuclei between the exposed - reference groups. Significantly faster repair kinetics of DNA-induced lesions was recorded for the first 2 h after gamma irradiation in the reference group compared to the exposed group. Using the threshold of 300 Bq/m3 for radon concentration, faster kinetics of DNA damage repair for people exposed to low radon concentrations, compared to those exposed to higher concentrations for the second phase of DNA repair kinetics was observed. CONCLUSION An increased radiosensitivity of lymphocytes, as well as slower repair kinetics, may be associated with exposure to higher indoor radon concentrations.

Seasonal variations of the radon and radium activity concentrations in karstic water sources originating in karstic formations were investigated as part of a premiere systematic survey conducted in Romania. A database including a total of 228 drinking water samples collected from 30 distinct water sources adjacent to rural communities was compiled. The radon and radium activity concentrations for all seasons, assessed based on solid scintillation, ranged from 2.1 to 19.7 Bq/L and from 0.6 to 3.0 Bq/L, respectively. Overall, the detected radon and radium contents did not exceed the radioprotection standards recommended by national and European legislation. However, in at least one season, the measured values for 31% of the samples exceeded the 11.1 Bq/L maximum contaminant level for radon in drinking water recommended by the Environmental Protection Agency of the United States. The associated radiological risk, reported in terms of annual effective dose, was calculated to be between 9.8 × 10−6 and 6.0 × 10−5 mSv/y for radon and between 5.9 × 10−5 and 2.7 × 10−4 mSv/y for radium, which are considerably below the WHO (World Health Organization) guidelines at a value of 0.1 mSv/y.

The present study aims to identify novel means of increasing the accuracy of the estimated annual indoor radon concentration based on the application of temporal correction factors to short-term radon measurements. The necessity of accurate and more reliable temporal correction factors is in high demand, in the present age of speed. In this sense, radon measurements were continuously carried out, using a newly developed smart device accompanied by CR-39 detectors, for one full year, in 71 residential buildings located in 5 Romanian cities. The coefficient of variation for the temporal correction factors calculated for combinations between the start month and the duration of the measurement presented a low value (less than 10%) for measurements longer than 7 months, while a variability close to 20% can be reached by measurements of up to 4 months. Results obtained by generalized estimating equations indicate that average temporal correction factors are positively associated with CO2 ratio, as well as the interaction between this parameter and the month in which the measurement took place. The impact of the indoor-outdoor temperature differences was statistically insignificant. The obtained results could represent a reference point in the elaboration of new strategies for calculating the temporal correction factors and, consequently, the reduction of the uncertainties related to the estimation of the annual indoor radon concentration. Acknowledgements: The research is supported by the project ID P_37_229, Contract No. 22/01.09.2016, with the title, Smart Systems for Public Safety through Control and Mitigation of Residential Radon linked with Energy Efficiency Optimization of Buildings in Romanian Major Urban Agglomerations SMART-RAD-EN” of the POC Programme.

Over the last 10 years applied scientific research has been carried out in Romania to tacked the residential radon issues. The increased interest to reduce the carbon footprint of buildings has lead to the implementation and use of new architectural solutions aimed to save energy in houses and other buildings. As a consequence, the degree of retrofit in existing buildings and energy efficiency of new buildings promoted the need to not only mitigate indoor radon, but improve indoor air quality overall. The present study found that the while the best performance in radon reduction was confirmed to be based on sub-slab depressurization (61% - 95% reduction), centralized and decentralized mechanical supply and exhaust ventilation with heat recovery yielded a good efficiency in overall improvement of indoor air quality (CO2, VOC, RH, temperature). The outcome of our research, as well as future perspectives, take into account the recommended harmonization of energy efficiency programs with those of public health by finding and applying the best technologies in compliance with energy saving and indoor environmental quality.

In the present study, the contemporary indoor radon activity concentration and the surface-deposited 210Po activity were measured in 65 Romanian dwellings. The activity of trapped 210Po on the glass surface was measured using the (CR-LR) difference technique. The retrospective radon activity concentration estimated on the basis of age-adjusted 210Po activity was found to have a geometric mean of 318 Bq m-3, being higher than the geometric mean (250 Bq m-3) of the contemporary indoor radon measurements, measured with two types of track detectors (RSKS and Radtrak2®). By applying Lin's concordance correlation coefficient, a substantial strength of agreement (rC = 0.94) was obtained between RSKS and Radtrak2® results, respectively a poor agreement (rC = 0.71) between the retrospective and contemporary methods. The ratio between contemporary and retrospective radon concentrations has a geometric mean of 0.8 and range from 0.2 to 3.9.

The purpose of this article is the assessment of energy efficiency and indoor air quality for a single-family house located in Cluj-Napoca County, Romania. The studied house is meant to be an energy-efficient building with thermal insulation, low U-value windows, and a high efficiency boiler. Increasing the energy efficiency of the house leads to lower indoor air quality, due to lack of natural ventilation. As the experimental campaign regarding indoor air quality revealed, there is a need to find a balance between energy consumption and the quality of the indoor air. To achieve superior indoor air quality, the proposed mitigation systems (decentralized mechanical ventilation with heat recovery combined with a minimally invasive active sub-slab depressurization) have been installed to reduce the high radon level in the dwelling, achieving an energy reduction loss of up to 86%, compared to the traditional natural ventilation of the house. The sub-slab depressurization system was installed in the room with the highest radon level, while the local ventilation system with heat recovery has been installed in the exterior walls of the house. The results have shown significant improvement in the level of radon decreasing the average concentration from 425 to 70 Bq/m3, respectively the carbon dioxide average of the measurements being around 760 ppm. The thermal comfort improves significantly also, by stabilizing the indoor temperature at 21 °C, without any important fluctuations. The installation of this system has led to higher indoor air quality, with low energy costs and significant energy savings compared to conventional ventilation (by opening windows).

A recent challenge in research dedicated to residential exposure to radon comes from the growing number of houses retrofitted to reduce energy consumption. Efficiently insulated buildings and modern architectural solutions can lead to the accumulation of high levels of indoor pollutants. A systematic analysis was conducted in a residential complex (consisting of six houses) in order to assess the annual radon concentration and to evaluate the intensity of the relationships with various factors, such as the indoor-outdoor temperature differences, wind speed and wind direction. Three types of occupational behaviour, influencing the ventilation rate of the dwellings and, implicitly, the indoor radon activity concentration were observed. By calculating the partial correlation coefficient between the radon concentration and the wind direction, with the wind speed as the control variable, for all six houses the correlation coefficient presents negative values.

It is generally accepted that radon emission is strongly influenced by the geological characteristics of the bedrock. However, transport in-soil and entry paths indoors are defined by other factors such as permeability, building and architectural features, ventilation, occupation patterns, etc. The purpose of this paper is to analyze the contribution of each parameter, from natural to man-made, on the radon accumulation indoors and to assess potential patterns, based on 100 case studies in Romania. The study pointed out that the geological foundation can provide a reasonable explanation for the majority of the values recorded in both soil and indoor air. Results also showed that older houses, built with earth-based materials, are highly permeable to soil radon. Energy-efficient houses, on the other hand, have a tendency to disregard the radon potential of the geological foundation, causing a higher predisposition to radon accumulation indoors and decreasing the general indoor air quality.

In the framework of the last Council Directive 2013/59 (Euratom, 2014) laying down basic safety standards for protection against the dangers arising from exposure to ionizing radiation, the problem of radon was assumed in Romania at national level by responsible authorities through the design and development of a National Radon Action Plan and an adequate legislation (HG nr. 526/2018). In order to identify radon risk areas, however, it is necessary to perform systematic radon measurements in different environmental media (soil gas, water, indoor air) and to map the results. This paper presents an atlas of up-to-date radon in soil and water levels for central and western part of Romania. The radon in soil map includes data from 2564 measurements carried out on-site, using Luk3C radon detector. The Luk-VR system was used to measure radon activity concentration from 2452 samples of drinking water. The average radon activity concentration was 29.3?kBq?m-3 for soil gas, respectively 9.8?Bq?l-1 for water dissolved air. Mapping of radon can be a useful tool to implement radon policies at both the national and local levels, defining priority areas for further study when land-use decisions must be made. This work was supported by the Romanian National Authority for Scientific Research and Innovation, CNCS-UEFISCDI, by the project ”Radon map (residential, geogenic, water) for center, west and north-west regions from Romania (RAMARO), PN-II-PCCA-PT-73/2012 and by the project ID P_37_229, Contract No. 22/01.09.2016, with the title „Smart Systems for Public Safety through Control and Mitigation of Residential Radon linked with Energy Efficiency Optimization of Buildings in Romanian Major Urban Agglomerations SMARTRAD-EN” of the POC Programme.

The indoor air quality (IAQ) was in the last decades a major topic of scientific research due to evidences of adverse effects of different chemicals on the human health. The retrofit of the existing buildings in order to increase the energy performance without proper implementation may have a negative effect on IAQ. This paper is focused on a comprehensive evaluation of indoor air pollutants in 100 retrofit houses from five Romanian cities, during cold season. Radon, volatile organic compounds (VOCs) and carbonyl compounds were determined using passive samplers. At the same time, continuous, real-time monitoring of radon and carbon dioxide concentrations, as well as indoor physical parameters, such as temperature, atmospheric pressure and relative humidity was carried out. In 95% of the houses the formaldehyde concentration was higher than the guidevalue. The most present volatile organic compounds were limonene, heptane, carbon tetrachloride, tetradecane and a-pinene. A statistically significant difference in radon concentration was observed between the two sampling methods, which highlights the importance of temporal variability of indoor pollutants. High radon values were correlated with the lack of adequate ventilation, an aspect underlined by high CO2 concentrations. Additionally, the study finds that indoor air pollutants can be attributed to three main factors: the physical characteristics and usage of the indoor environment (volatile organic compounds and carbonyl compounds), the properties of the sub-slab soil (radon) and the occupational factor (CO2). Each factor should be treated separately when indoor air quality management is addressed.

The present study reports by experimental measurements the levels of volatile organic compounds (VOC) and radon concentrations in two high school classrooms. The difference between the two classrooms abbreviated as S7 and S8 is the presence of a mechanical ventilation system in one of them (S8). The topic is of high importance as the indoor air quality (IAQ) can affect the health and learning performance of the pupils. VOC and RADON have a substantial weight of IAQ and a study of theirs level can help us to better understand at what levels the children are exposed daily. The two mentioned classroom have suffered recently a massive renovation. The classroom with the ventilation system was mounted with variable flow of fresh air, while the other one relies only on natural ventilation by opening the windows. In S8 several test were done for different fresh air ventilation rates. The conclusions were that in the classroom S7 there were measured high levels of VOC and radon, higher than the maximum admissible which is a serious problem. On the other hand, in the classroom S8 for all the different air rates the values were very low, thus creating a healthy and comfortable environment for the learning process. In this article we raise again the problem of lack of a mechanical ventilation system in all Romanian schools. Due to thermal rehabilitation of the buildings the building is air sealed with negative impact on the introduced fresh air. Moreover, the high number of occupants in the classroom and the renovation of classroom have negative influence on all indoor pollutants levels. The only solution to these problems is a correct design of a school or to install other ways to mechanically introduce the fresh air.

The purpose of the article is to evaluate the annual effective dose for 80 women divided into two samples; one sample located in the former uranium Băiţa-Ştei area, hereinafter referred to as case sample, respectively for a control sample, located in the same county, but exposed in most cases to indoor radon activity concentrations

Measurements have been carried out using four types of passive detectors in four of the most popular show caves in Romania. Three types of detectors (RSKS, RadTrak and CD) were used for radon measurements and two (Raduet and CD) for thoron measurement. Activity concentrations in air were measured in the same locations for two seasons, autumn and winter. Measured values for the different caves varied between below detection limit (5 Bq m-3) and 4024 Bq m-3 for radon and from below 10 to 583 Bq m-3 for thoron. The results indicate a very good correlation between RSKS and RadTrak detectors (r = 0.96). The most significant difference between radon concentrations measured with different types of detectors (RSKS and CD) was higher than 150%. The study suggests that the activity concentration of radon in caves, measured using track detectors, could not be influenced by the type of detector used if the microclimate factor is acknowledged.

In September 2016, the SMART-RAD-EN Project was launched, funded by the Competitiveness Operational Programme 2014-2020 of Romania, to be developed up to the year 2020. The ambitious overall objective is focus on to the concept of "smart city" in terms of intelligent integrated solutions, which it aims to achieve in international premiere, in order to improve public health by increasing indoor environmental quality and optimizing the energy efficiency of housing in five urban areas of Romania. In this study, the current status of the development of the prototype system for continuous monitoring and remote data transmission on radon levels and other household air pollutants (CO2, CO, VOCs and temperature, pressure and humidity sensors) is presented, as an important objective in the frame of the SMART_RAD_EN project. The prototype for intelligent monitoring system was assembled and now is involved in the testing process. Metrology and quality assurance of the prototype system will be carried out within accredited European laboratories and by consulting with the international scientific experts. Remote data transmitted will allow: (1) real time interactive visualization, of the impact of user behavior on indoor air quality; (2) information in case of exceeding threshold levels; (3) to produce estimates of future pollution as a result of the correlation with the meteorological parameters (temperature, pressure and humidity) and user activity. The system, metrological validated, will be implemented in 100 houses with high exposure to radon and other ambient pollutants from the main Romanian urban agllomerations ? Cluj-Napoca, Bucuresti, Timisoara, Iasi and Sibiu.

Radon is a natural radioactive gas that occurs due to the radioactive decay of radium (226Ra) present in rocks which, in turn, cames from the radioactive decay of uranium (238U), a primordial natural element. Along with factors such as porosity, permeability and humidity of the rocks and soils, pressure and temperature, geology setting plays one of the most important roles in the release of radon into the environment. Depending on the mineralogical compositions and characteristics of the bedrock from a certain area, a higher or lower concentration of radioactive minerals can be found in the rocks, which will directly influence the level of radon in the atmosphere, implicitly the concentration of radon measured in houses. In this study, a six months concentration of radon (222Rn) was assessed in 256houses from Cluj-Napoca area using CR-39 nuclear track detectors. Correlations between the indoor radon levels and the geological setting was further analyzed. The aim is to better understand the influence of geology on the concentration of radon levels in homes, in order to further identify other risk areas in terms of exposure to radon. Further investigation is needed on other factors influencing the accumulation of radon in high concentrations indoor, such as ventilation, occupation patterns or constructive and architectural features for typical houses. Therefore, the results of this work are considered to be important for indoor radon management in Romania.

Outdoor gamma radiation measurements in Cluj County, Romania have been performed using solid-state thermoluminescent detectors in order to develop a high-resolution database for natural gamma dose rates. Integrated measurements have been carried out for an exposure time of minimum 3 weeks. According to European Union requirements, the territory has been divided into 69 grids of 10 × 10 km. The cells were monitored using LiF:Mg,Cu,P detectors. For two locations the results were 136 ± 7 and 150 ± 7 nGy h(-1), respectively. These results can be explained by the existent geological substrate. The values ranged from 56 ± 4 to 150 ± 7 nGy h(-1), with an average value of 91 ± 2 nGy h(-1), being in agreement with the 2008 United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR) Report. A high-resolution map of gamma dose rates in Cluj County is presented for the first time.

The paper presents the results of a combined soil and vegetation survey in Rosia Montana mining area (western Romania), famous for its gold and silver deposits, extensively exploited over the last 2,000 years. As the ore extraction has ceased in 2006 and new operations could be initiated in the future, the study contributes to the definition of the environmental baseline. Samples of topsoil and leaves of the tree species Betula pendula and Carpinus betulus have been collected from the inside and outside of the mining area, on a total surface of more than 60 km2. The pH and heavy metal concentrations (Cd, Cr, Cu, Ni, Pb, and Zn) have been measured on 262 soils/sediments samples, revealing the predominantly acidic character of soils and the generally low contents of heavy metals. Stronger acidity and higher contents of heavy metals have been noticed in the proximity of the mining site, on the tailings and waste rock dumps, and along the streams with acid water. More than 100 leaf samples have been analysed for the same heavy metals as soils and also for chlorophyll fluorescence and pigment concentrations. B. pendula has shown a particular ability to concentrate Zn in leaves, at levels that may greatly exceed the Zn content in the corresponding soil samples. The correlation between the heavy metal contents in leaves and in soils, in most of the cases, is not very strong, presumably in relation to the low concentrations in soils. The chlorophyll concentration in leaves of B. pendula slightly diminishes on soils with low pH.

The radon concentration was measured in the drinking water of public water supply and private wells located in the mining area of BăiŢa-Ştei, Bihor County, Romania. The measurements were performed using the LUK-VR system based on radon gas measurement with Lucas cell. The results show that the radon concentrations are within the range of 1.9-134.3 kBq m(-3) with an average value of 35.5 kBq m(-3) for well water, 18.5 kBq m(-3) for spring water and 6.9 kBq m(-3) for tap water. Comparing with previous data from the whole of Transylvania, the average value is two times higher, proving this zone to be a radon-prone area. From the results of this study the effective dose to the population is between 4.78 and 338.43 µSv y(-1). These doses are within the recommended limits of the world organisations.

Radon represents the most important contribution of population exposure to natural ionising radiation. This article presents the first indoor radon map in some regions of Romania based on 883 surveyed buildings in the Ştei-BăiŢa radon-prone region and 864 in other regions of Romania. Indoor radon measurements were performed in the last 10 y by using CR-39 nuclear track detectors exposed for 3-12 months on ground floor levels of dwellings. Excluding the Ştei-BăiŢa radon-prone region, an average indoor radon concentration of 126 Bq m(-3) was calculated for Romanian houses. In the Ştei-BăiŢa radon-prone area, the average indoor concentration was 292 Bq m(-3). About 21 % of the investigated dwellings in the Ştei-BăiŢa radon-prone region exceed the threshold of 400 Bq m(-3), while 5 % of the dwellings in other areas of Romania exceed the same threshold. As expected, indoor radon concentration is not uniformly distributed throughout Romania. The map shows a high variability among surveyed regions, mainly due to the differences in geology. The radon emanation rate is substantially influenced by the soil characteristics, such as the soil permeability and soil gas radon concentration. Since higher permeability enables the increased migration of soil gas and radon from the soil into the building, elevated levels of indoor radon can be expected in more permeable soil environments.

Admission Rockall score (RS), full RS, and Glasgow-Blatchford Bleeding Score (GBS) can all be used to stratify the risk in patients presenting with upper gastrointestinal bleeding (UGIB) in the emergency department (ED). The aim of our study was to compare both admission and full RS and GBS in predicting outcomes at UGIB patients in a Romanian ED.A total of 229 consecutive patients with UGIB were enrolled in the study. Patients were followed up 60 days after admission to ED because of UGIB episode to determine cases of rebleeding or death during this period. By using areas under the curve (AUCs), we compared the 3 scores in terms of identifying the most predictive score of unfavorable outcomes.Rebleeding rate was 40.2% (92 patients), and mortality rate was 18.7% (43 patients). For the prediction of mortality, full RS was superior to GBS (AUC, 0.825 vs 0.723; P = .05) and similar to admission RS (AUC, 0.792). Glasgow-Blatchford Bleeding Score had the highest accuracy in detecting patients who needed transfusion (AUC, 0.888) and was superior to both the admission RS and full RS (AUC, 0.693 and 0.750, respectively) (P.0001). In predicting the need for intervention, the GBS was superior to both the admission RS and full RS (AUC, 0.868, 0.674, and 0.785, respectively) (P.0001 and P = .04, respectively).The GBS can be used to predict need for intervention and transfusion in patients with UGIB in our ED, whereas full RS can be successfully used to stratify the mortality risk in these patients.

The exposure to radon and radon decay products in homes and at workplaces represents the greatest risk from natural ionizing radiation. The present study brings forward the residential, soil and water radon surveys in 5 counties of Romania. Indoor radon measurements were performed by using CR-39 track detectors exposed for 3 months on ground-floor level of dwellings, according to the NRPB Measurements Protocol. Radon concentrations in soil and water were measured using the LUK3C device. The indoor radon concentrations ranged from 5 to 2592 Bq⋅m−3 with an updated preliminary arithmetic mean of 133 Bq⋅m−3, and a geometric mean of 90 Bq⋅m−3. In about 6% of the investigated grid cells the indoor radon concentrations exceed the threshold of 300 Bq⋅m−3. The soil gas radon concentration varies from 0.8 to 169 kBq⋅m−3, with a geometric mean of 28.4 kBq⋅m−3. For water samples, the results show radon concentrations within the range of 0.3–352 kBq⋅m−3 with a geometric mean of 7.7 Bq⋅L−1. The indoor radon map was plotted on a reference grid developed by JRC with the resolution 10 × 10 km2.

A comprehensive radon survey has been carried out in Băiţa radon-prone area, Transylvania, Romania, in 4 localities (Băiţa, Nucet, Fînaţe, and Cîmpani) situated in the vicinity of former Romanian uranium mines. Indoor radon concentrations have been measured in 1128 ground floor rooms and cellars of 303 family houses by using CR-39 diffusion type radon detectors. The annual average of indoor radon concentration for Băiţa area was found to be 241±178 Bq m(-3), which is about two times higher than the average value of 126 Bq m(-3), computed for Romania. About 28% of investigated houses exceed the reference level of radon gas in dwellings of 300 Bq m(-3). The indoor radon measurements on each house have been carried out in several rooms simultaneously with the aim of obtaining a more detailed picture on the exposure to radon in the studied area. An analysis on the variability of radon levels among floors (floor-to-floor variation) and rooms (room-to-room variation) and also the influence of factors like the presence of cellar or the age of the building is presented. The coefficient of variation (CV) within ground floor rooms of the same house (room-to-room variation) ranged between 0.9 and 120.8%, with an arithmetic mean of 46.2%, a large variability among rooms within surveyed dwellings being clearly identified. The mean radon concentration in bedrooms without cellar was higher than in bedrooms above the cellar, the difference being statistically significant (t test, one tail, p0.001, n=82). For houses built during 1960-1970 an increasing trend for radon levels was observed, but overall there was no significant difference in indoor radon concentrations by age of dwelling (one-way ANOVA test, p0.05).

Radon and radon progeny are present indoors, in houses and others dwellings, representing the most important contribution to dose from natural sources of radiation. Most studies have demonstrated an increased risk of lung cancer at high concentration of radon for both smokers and nonsmokers. The work presents a comparative analysis of the radon exposure data in the two radon-prone areas, Stei, Transylvania, (Romania), in the near of old Romanian uranium mines and in the granitic area of Torrelodones town, Sierra de Guadarrama (Spain). Measurements of indoor radon were performed in 280 dwellings (Romania) and 91 dwellings (Spain) by using nuclear track detectors, CR 39. The highest value measured in Stei area was 2650 Bq m(-3) and 366 Bq m(-3) in the Spanish region. The results are computed with the BEIR VI report estimates using the age-duration model at an exposure rate below 2650 Bq m(-3). We used the EC Radon Software to calculate the lifetime lung cancer death risks for individuals groups in function of attained age, radon exposures and tobacco consumption. A total of 233 lung cancer deaths were observed in the Stei area for a period of 13 years (1994-2006), which is 116.82% higher than expected from the national statistics. In addition, the number of deaths estimated for the year 2005 is 28, which is worth more than 2.21 times the amount expected by authorities. In comparison, for Torrelodones was rated a number of 276 deaths caused by lung cancer for a period of 13 years, which is 2.09 times higher than the number expected by authorities. For the year 2005 in the Spanish region were reported 32 deaths caused by pulmonary cancer, the number of deaths exceeding seen again with a factor of 2.10 statistical expectations. This represents a significantly evidence that elevated risk can strongly be associated with cumulated radon exposure.

Radium concentration was determined in 23 types of bottled mineral waters, commercially available in Romania. Using the Lucas cells with the device Luk 3A, the radium concentration was determined directly, after the radon balanced with radium. From these measurements, the corresponding annual effective ingestion dose was determined.

Radon is a radioactive noble gas of a natural origin that may be found anywhere in soil, air and in different types of water: surface, well and spring. It is worth to carry out surveys for the radon in natural waters for radiation protection as well as for geological considerations. The results present here are from a survey carried out in the Transylvania region in Romania for radon concentrations in natural waters. The measurements were made using the LUK-VR system that is based on radon gas measurement with Lucas cell. Due to the large number of water samples (1511 samples) collected for analysis, a short counting time had to be used, so that the measurements were made during the non-equilibrium state between radon and its progeny. The results show that the radon concentrations are within the range of 0.5 – 129.3 kBq / m 3 with an average value of 15.4 kBq / m 3 for all types of water covered within this survey.

The objective of the present study was to assess the lung cancer risk induced by exposures to radon progeny of people living in some areas of Transylvania, Romania. Indoor radon concentrations were measured in 667 dwellings of Stei area, Cluj, Bistrita-Nasaud, Sibiu, and Alba counties. Measurements were performed using CR-39 track detectors, exposed for a minimum of 3 mo. Average annual radon concentrations were 232, 114, 71, 62, and 161 Bq m -3 for Stei area, Cluj, Bistrita-Nasaud, Sibiu, and Alba, respectively. The linear risk model of Darby was used to simulate the dose-effect relationship and relative lung cancer risk at low doses of alpha particles specific to residential radon exposures. Predicted relative risks at the measured exposure levels, together with information on the total number of reported lung cancer deaths and the number of people living in these regions, enabled us to estimate the fraction of lung cancer cases in each area that is attributable to radon. These percentages are 16.67% for Stei area, 9.09% for Cluj, 5.66% for Bistrita-Nasaud, 4.76% for Sibiu, and 12.28% for Alba county among lifetime non-smokers. Assuming that the smoking rates are similar for the investigated regions (10.72% smokers among men and 5.95% among women), around 64 to 69% of the total number of annual lung cancer deaths, stratified by sex, would be attributed to radon and occur among smoking male population, and around 35 to 44% would be attributed to radon and occur among smoking female population.

Aims and Background Brain metastases confer a worse prognosis to breast cancer because they determine a severe increase in mortality. The aim of this study was to identify the early symptoms in patients with brain metastases after breast cancer treatment and to evaluate the median survival rate in women with single and operable brain lesions. Patients and Methods We examined 43 patients with brain metastases secondary to breast cancer treated in the Oncological Institute Prof I Chiricuţă, Cluj-Napoca, during the period 2000-2006. Results The median interval between the breast cancer diagnosis and detection of central nervous metastases was 21 months. The most frequent symptoms were headache, gait disturbance, nausea and vomiting. Patients with a single brain lesion had a median survival of 23 months compared to only 7 months in case of patients with multiple brain metastases. Conclusions The prognosis is worse in patients with solitary brain metastases secondary to breast cancer than in patients who present extracranial metastases. Among factors considered favorable in these patients are a single brain lesion, accessibility to surgery, and the absence of associated extracranial metastases.

Measurements of indoor radon concentrations were performed in 406 residential houses in five counties (Cluj, Bihor, Alba, Bistrita, and Sibiu) using Makrofol and CR-39 alpha-track detectors. From our measurements, an average indoor radon concentration of 82.5 Bq m(-3) for the Transylvanian population was calculated, i.e. an annual effective dose of 2.4 mSv for the whole body. The calculated dose is 62% higher than that previously reported but yet below the recommended action level of ICRP (International Commission on Radiological Protection). A log-normal distribution of the radon concentration was obtained for the studied counties, excluding some higher values from the Stei region--a radon-prone area in Transylvania. For the Stei region, the data show a dual log-normal distribution of the radon concentration with the second maximum being related to the houses built using uranium waste as a construction material. Assuming a cancer risk coefficient of 10(-4)/100 Bq m(-3) year(-1), one can estimate that about 600 lung cancer per year, for about 7,000,000 inhabitants of the Transylvania region, are due to radon inhalation.

Plant‐derived antioxidants due to their phenolic compounds content are reported as potential candidates for reducing the levels of oxidative stress in living organisms. Grape seed extracts are very potent antioxidants and exhibit numerous interesting pharmacologic activities. Hydroethanolic (50/50, v/v) standardized extract was obtained from red grape seed (Vitis vinifera, variety Burgund Mare—BM). The total polyphenols content was evaluated by Folin‐Ciocalteu procedure and expressed as μEq Gallic Acid/ml. The aim of this study was to evaluate the potential antioxidant effects of different concentrations of BM extract against 60Co γ‐rays induced DNA damage in human lymphocytes. Samples of human lymphocytes were incubated with BM extract (12.5, 25.0 and 37.5 μEq GA/ml, respectively) administered at 30 minutes before in vitro irradiation with γ‐rays (2 Gy). The DNA damage and repair in lymphocytes were evaluated using alkaline comet assay. Using the lesion score, the radiation‐induced DNA damage was found t...