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Abstract Objectives To investigate the relationships between life events and psychological distress in the postepidemic era as well as the effects of fear of COVID-19 (FCV-19) and impermanence on these relationships to enrich the study of the underlying psychological mechanisms of postepidemic psychological distress and to provide a theoretical basis for scientific prevention and intervention in individuals with psychological distress. Methods A survey of 504 adults (71.3% female; age M = 26.87, SD = 10.70) was conducted via the Social Readjustment Rating Scale, the FCV-19 Scale, the Impermanence Scale, the Anxiety Scale and the Depression Scale, and a structural equation model was established to explore the relationships between variables. Results The present study revealed the following: (1) there is a significant positive correlation between life events and psychological distress; (2) FCV-19 completely mediates the relationship between life events and psychological distress; and (3) impermanence moderates the mediation, regulating the path by which life events affect FCV-19 and the path by which FCV-19 affects psychological distress. Conclusions In the postepidemic era, impermanence can effectively mitigate the impact of life events on FCV-19 and the impact of FCV-19 on psychological distress.

Abstract Polymer nanocomposites with tuning functions are exciting candidates for various applications, and most current research has focused on static mechanical reinforcement. Actually, under service conditions of complex dynamic interference, stable dynamic mechanical properties with high energy dissipation become more critical. However, nanocomposites often exhibit a trade-off between static and dynamic mechanics, because of their contradictory underlying physics between chain crosslinking and chain relaxation. Here, we report a general strategy for constructing ultra-stable dynamic mechanical complex fluid nanocomposites with high energy dissipation by infusing complex fluids into the nanoconfined space. The key is to tailor full-scale polymer dynamics across an exceptionally broad timescale by single-chain confinement. These materials exhibit stable storage modulus (100 ~ 102 MPa) with high energy dissipation (loss factor > 0.4) over a broad frequency range (10−1 ~ 107 Hz)/temperature range (−35 ~ 85°C). In the loss factor > 0.4 region, their dynamic mechanical stability (rate of modulus change versus temperature (k)) is 10 times higher than that of conventional polymer nanocomposites.

Variable air volume air-conditioning system (VAV system) has multiple control loops that interfered with each other, which has serious impacts on its actual operation effect. To solve this problem, the index of global hydraulic stability, which takes the physical quantity of the pipe network pressure and the air flow rate as reference, is proposed based on Graph theory, and a pipe network model for the hydraulic calculation is built. Then, the simulation study based on the actual operation data is carried out in MATLAB. The distribution of the pressure and the air flow in the pipe network is obtained, and the action interferences among adjacent terminal boxes are analyzed based on global hydraulic stability under damper position regulation strategy (DP strategy) and fan frequency regulation strategy (FF strategy). Results indicate that the FF strategy can decrease the total change distance of the damper position compared with the DP strategy, and then the fluctuation of the pressure and the air flow can be reduced. The contribution of this study is to provide an evaluation index of global hydraulic stability, which can also be used in other air-conditioning systems with multiple terminals and multiple control loops.

Current climate change, particularly ocean warming, will induce shifts in marine species distribution and composition, affecting the marine food web and, thus, trophic interactions. Analyses of the stable isotopes 13C and 15N are commonly used to detect trophic markers for food web analyses. With the current standard methods used in food web ecology, it is still challenging to identify potential changes in the uptake and utilization of trophic markers. In this work, we present a 13C-enrichment analysis by NMR spectroscopy to track the uptake and utilization of dietary carbon in a simple laboratory experiment of a primary producer and its consumer (algae and bivalve). In particular, we tested the hypothesis of a temperature-dependent use of dietary carbon by tracing the incorporation of 13C-atoms. Unicellular phytoplankton, Phaeodactilum tricornutum, was reared in a medium containing 13C-labeled bicarbonate. The accompanying 13C-NMR spectra of labeled P. tricornutum showed a specific profile of 13C-labeled compounds, including typical trophic markers such as the polyunsaturated omega-3 fatty acid eicosapentaenoic acid (EPA). Afterwards, 13C-labeled P. tricornutum was fed to King scallops, Pecten maximus, kept at two different temperatures (15°C and 20°C). Tissue-specific NMR spectra of P. maximus revealed elevated 13C-NMR signals, particularly of the fatty acid EPA in the digestive gland, which was not evident in muscle tissue. The comparison between the two temperatures indicated a change in trophic markers. At the higher temperature, less unsaturated fatty acids were detected in the digested gland, but increased 13C-labels in sugars were detected in the adductor muscle. This might indicate a change in the uptake and utilization of the trophic marker EPA in P. maximus due to a shift in energy conversion from favored beta-oxidation at colder temperatures to conversion from carbohydrates in the warmth. Our approach indicates that besides the accumulation of trophic markers, their incorporation and conversion are additional important factors for the reliable interpretation of trophic linkages under climate change.

Abstract Electroencephalography (EEG) microstate analysis is a neuroimaging analytical method that has received considerable attention in recent years and is widely used for analysing EEG signals. EEG is easily influenced by internal and external factors, which can affect the repeatability and stability of EEG microstate analysis. However, there have been few reports and publicly available datasets on the repeatability of EEG microstate analysis. In the current study, a 39-year-old healthy male underwent a total of 60 simultaneous electroencephalography and electrocardiogram measurements over a period of three months. After the EEG recording was completed, magnetic resonance imaging (MRI) was also conducted. To date, this EEG dataset has the highest number of repeated measurements for one individual. The dataset can be used to assess the stability and repeatability of EEG microstates and other analytical methods, to decode resting EEG states among subjects with open eyes, and to explore the stability and repeatability of cortical spatiotemporal dynamics through source analysis with individual MRI.

Functional gastrointestinal disorders (FGIDs) and inflammatory bowel disease (IBD) are common clinical disorders characterized by recurrent diarrhea and abdominal pain. Although their pathogenesis has not been fully clarified, disruptions in intestinal motility and immune function are widely accepted as contributing factors to both conditions, and the brain–gut axis plays a key role in these processes. Traditional Chinese Medicine (TCM) employs a holistic approach to treatment, considers spleen and stomach impairments and liver abnormality the main pathogenesis of these two diseases, and offers a unique therapeutic strategy that targets these interconnected pathways. Clinical evidence shows the great potential of TCM in treating FGIDs and IBD. This study presents a systematic description of the pathological mechanisms of FGIDs and IBD in the context of the brain–gut axis, discusses clinical and preclinical studies on TCM and acupuncture for the treatment of these diseases, and summarizes TCM targets and pathways for the treatment of FGIDs and IBD, integrating ancient wisdom with contemporary biomedical insights. The alleviating effects of TCM on FGID and IBD symptoms are mainly mediated through the modulation of intestinal immunity and inflammation, sensory transmission, neuroendocrine–immune network, and microbiota and their metabolism through brain–gut axis mechanisms. TCM may be a promising treatment option in controlling FGIDs and IBD; however, further high-quality research is required. This review provides a reference for an in-depth exploration of the interventional effects and mechanisms of TCM in FGIDs and IBD, underscoring TCM’s potential to recalibrate the dysregulated brain–gut axis in FGIDs and IBD.

Abstract Pyroptosis may play an important role in the resistance of ovarian cancer (OC) to chemotherapy. However, the mechanism by which pyroptosis modulation can attenuate chemotherapy resistance has not been comprehensively studied in OC. Here, we demonstrated that RAS‐associated C3 botulinum toxin substrate 1 (RAC1) is highly expressed in OC and is negatively correlated with patient outcomes. Through cell function tests and in vivo tumor formation tests, we found that RAC1 can promote tumor growth by mediating paclitaxel (PTX) resistance. RAC1 can mediate OC progression by inhibiting pyroptosis, as evidenced by high‐throughput automated confocal imaging, the release of lactate dehydrogenase (LDH), the expression of the inflammatory cytokines IL‐1β/IL‐18 and the nucleotide oligomerization domain‐like receptor family pyrin domain‐containing 3 (NLRP3) inflammasome. Mechanically, RNA‐seq, gene set enrichment analysis (GSEA), coimmunoprecipitation (Co‐IP), mass spectrometry (MS), and ubiquitination tests further confirmed that RAC1 inhibits caspase‐1/gasdermin D (GSDMD)‐mediated canonical pyroptosis through the P21‐activated kinase 4 (PAK4)/mitogen‐activated protein kinase (MAPK) pathway, thereby promoting PTX resistance in OC cells. Finally, the whole molecular pathway was verified by the results of in vivo drug combination tests, clinical specimen detection and the prognosis. In summary, our results suggest that the combination of RAC1 inhibitors with PTX can reverse PTX resistance by inducing pyroptosis through the PAK4/MAPK pathway.

Traditional impact-resistance materials relying on the combination of supporting materials and energy-dissipation elastomers can effectively reduce shock load, yet the sharp interface between two types of materials causes discontinuous stress transfer and cracking. Here, inspired by the squid beak, we report a type of high impact-resistance gradient elastomers with large-scale modulus gradient with about three orders of magnitude (modulus range of 7 × 103 ∼ 7 × 106 Pa) and high energy dissipation (loss factor > 0.6) over a wide temperature range by diffusively introducing stiff polymers in a highly damping elastomer with controlled mechanical properties. Under the action of an external force, our gradient elastomers exhibit soft-while-stiff attributes, combining cushioning and support. In drop hammer impact tests, our gradient materials can reduce impact strength by 80 %, significantly better than commercial protective gear. It is worth mentioning that the modulus of the bottom layer matches that of the tissues for better protection.

Shale gas, mainly consisting of adsorbed gas and free gas, has served a critical role of supplying the growing global natural gas demand in the past decades. Considering that the adsorbed methane has contributed up to 80% of the total gas in place (GIP), understanding the methane adsorption behaviors is imperative to an accurate estimation of total GIP. Historically, the single-site Langmuir model, with the assumption of a homogeneous surface, is commonly applied to estimate the adsorbed gas amount. However, this assumption cannot depict the methane adsorption characteristics due to various compositions and pore sizes of shales. In this work, a multi-site model integrating the energetic heterogeneity in adsorption is derived to predict methane adsorption on shale. Our results show that the multi-site model is capable of addressing the heterogeneity of shales by a wide range of adsorption energy distributions (owing to the complex compositions and different pore sizes), which is different from the single-site model only characterized by single adsorption energy. Consequently, the multi-site model results have better accuracy against the experimental data. Therefore, applying the multi-site Langmuir model for estimating GIP in shales can achieve more accurate results compared with using the traditionally single-site model.

Abstract The morphological variation in Schizothorax oconnori, Schizothorax waltoni, and their natural hybrids was examined using conventional and image‐based analysis approaches. In total, 38 specimens of S. oconnori, 35 of S. waltoni, and 37 natural hybrids were collected from the Shigatse to the Lhasa section of the Yarlung Zangbo River during June and July 2021. A total of 21 morphometric, 4 meristic, and 27 truss variables were employed for the classification of S. oconnori, S. waltoni, and natural hybrids. Principal component analysis (PCA) and factor analysis (FA), as well as discriminant function analysis (DFA) and cluster analysis (CA), were conducted to identify differences based on traditional and truss measurements. Four principal components explained 75.92% of the variation among the morphometric characters, while five principal components accounted for 79.69% of the variation among the truss distances. FA results showed that factor 1 was associated with head shape, and factor 2 was associated with fins based on morphometric characters. Among the truss characters, factor 1 was related to head shape, and factor 2 was related to chest shape. In DFA, morphometric measurements achieved higher accuracy (100%) compared to truss distances (94.55%). The head morphology of hybrids exhibited intermediate traits between S. oconnori and S. waltoni. Both morphometry‐based and truss‐based clustering indicated that the morphology of natural hybrids leaned toward S. oconnori. In conclusion, the combination of morphometric and truss analysis is beneficial for classifying S. oconnori, S. waltoni, and their natural hybrids. The presence of natural hybrids could be considered an evolutionary response to the differentiation of nutritional and spatial niches in the middle Yarlung Zangbo River.

Expansive soil is widely distributed and often needs to be improved for engineering and construction needs. Using blast furnace slag and fly ash as precursors and NaOH as an alkali activator, a geopolymer was prepared to solidify expansive soil, and the effect of nano-SiO2 on the compressive strength and water stability of the geopolymer-solidified expansive soil was further studied. The effects of alkali addition ratio, nano-SiO2 addition ratio, and curing agent addition ratio on the unconfined compressive strength and water stability of the cured soil were studied through unconfined compressive strength tests, and the curing mechanism was analyzed by electron microscopy scanning. The experimental results showed that the unconfined compressive strength and water stability of geopolymer-stabilized soil first increased and then decreased with an increase in alkali activator dosage. The optimal dosage of alkali activator was found to be 12.5%. Furthermore, it was found that adding nano-SiO2 can further enhance the strength and water stability of solidified soil. When the content of nano-SiO2 was 3%, the unconfined compressive strength was increased by 15%. With an increase in the content of nano-SiO2 doped polymer (GFNS), the unconfined compressive strength and water stability of the solidified soil showed a trend of first increasing and then decreasing, reaching a peak at a content of 20%. The cementitious materials, such as hydrated calcium silicate and hydrated calcium silicate aluminate, generated by the reaction between nano-SiO2 and geopolymer played a role in bonding and filling in the solidified soil. Under the joint action of the two, the structural arrangement between the solidified soil particles became more compact, which improved the strength of the solidified soil.

The annual co-circulation of two influenza A subtypes, H1N1 and H3N2, viruses in humans poses significant public health threats worldwide. However, the continuous antigenic drift and shift of influenza viruses limited the effectiveness of current seasonal influenza vaccines, necessitating the development of new vaccines against both seasonal and pandemic viruses. One potential solution to this challenge is to improve inactivated vaccines by including multiple T-cell epitopes. In this study, we designed stabilized trimeric recombinant mosaic HA proteins named HAm, which contain the most potential HA T-cell epitopes of seasonal influenza A virus. We further evaluated the antigenicity, hemagglutinin activity, and structural integrity of HAm and compared its immunogenicity and efficacy to a commercial quadrivalent inactivated influenza vaccine (QIV) in mice. Our results demonstrated that the HAm vaccine was able to induce broadly cross-reactive antibodies and T-cell responses against homologous, heterologous, and heterosubtypic influenza-naive mice. Additionally, the HAm antigens outperformed QIV vaccine antigens by eliciting protective antibodies against panels of antigenically drifted influenza vaccine strains from 2009 to 2024 and protecting against ancestral viruses’ lethal challenge. These results suggest that the HAm vaccine is a promising potential candidate for future universal seasonal and pandemic influenza vaccine development.

Understanding the mechanisms of snap-off during gas–liquid immiscible displacement is of great significance in the petroleum industry to enhance oil and gas recovery. In this work, based on the original pseudo-potential lattice Boltzmann method, we improved the fluid–fluid force and fluid–solid force scheme. Additionally, we integrated the Redlich–Kwong equation of state into the lattice Boltzmann model and employed the exact difference method to incorporate external forces within the lattice Boltzmann framework. Based on this model, a pore–throat–pore system was built, enabling gas–liquid to flow through it to investigate the snap-off phenomenon. The results showed the following: (1) The snap-off phenomenon is related to three key factors: the displacement pressure, the pore–throat length ratio, and the pore–throat width ratio. (2) The snap-off phenomenon occurs only when the displacement pressure is within a certain range. When the displacement pressure is larger than the upper limit, the snap-off will be inhibited, and when the pressure is less than the lower limit, the gas–liquid interface cannot overcome the pore–throat and results in a “pinning” effect. (3) The snap-off phenomenon is controlled using the pore–throat structures: e.g., length ratio and the width ratio between pore and throat. It is found that the snap-off phenomenon could easily occur in a “long-narrow” pore–throat system, and yet hardly in a “short-wide” pore–throat system.

Abstract The development of a universal influenza vaccine to elicit broad immune responses is essential in reducing disease burden and pandemic impact. In this study, the mosaic vaccine design strategy and genetic algorithms were utilized to optimize the seasonal influenza A virus (H1N1, H3N2) hemagglutinin (HA) and neuraminidase (NA) antigens, which also contain most potential T-cell epitopes. These mosaic immunogens were then expressed as virus-like particles (VLPs) using the baculovirus expression system. The immunogenicity and protection effectiveness of the mosaic VLPs were compared to the commercial quadrivalent inactivated influenza vaccine (QIV) in the mice model. Strong cross-reactive antibody responses were observed in mice following two doses of vaccination with the mosaic VLPs, with HI titers higher than 40 in 15 of 16 tested strains as opposed to limited cross HI antibody levels with QIV vaccination. After a single vaccination, mice also show a stronger level of cross-reactive antibody responses than the QIV. The QIV vaccinations only elicited NI antibodies to a small number of vaccine strains, and not even strong NI antibodies to its corresponding vaccine components. In contrast, the mosaic VLPs caused robust NI antibodies to all tested seasonal influenza virus vaccine strains. Here, we demonstrated the mosaic vaccines induces stronger cross-reactive antibodies and robust more T-cell responses compared to the QIV. The mosaic VLPs also provided protection against challenges with ancestral influenza A viruses of both H1 and H3 subtypes. These findings indicated that the mosaic VLPs were a promising strategy for developing a broad influenza vaccine in future.

Abstract Background Surgical site infection (SSI) is the most common complications in spinal surgery. In SSI, non-superficial surgical site infections are more likely to result in poor clinical outcomes. It has been reported that there are multiple factors contributing to postoperative non-superficial SSI, but still remains controversial. Therefore, the aim of this meta-analysis is to investigate the potential risk factors for non-superficial SSI following spinal surgery. Methods A systematic database search of PubMed, Embase, Web of Science, Cochrane Library and Clinical Trials was performed for relevant articles published until September 2022. According to the inclusion and exclusion criteria, two evaluators independently conducted literature screening, data extraction and quality evaluation of the obtained literature. The Newcastle–Ottawa Scale (NOS) score was used for quality evaluation, and meta-analysis was performed by STATA 14.0 software. Results A total of 3660 relevant articles were initially identified and 11 articles were finally included in this study for data extraction and meta-analysis. The results of meta-analysis showed that the diabetes mellitus, obesity, using steroids, drainage time and operative time were related to the non-superficial SSI. The OR values (95%CI) of these five factors were 1.527 (1.196, 1.949); 1.314 (1.128, 1.532); 1.687(1.317, 2.162); 1.531(1.313, 1.786) and 4.255(2.612, 6.932) respectively. Conclusions Diabetes mellitus, obesity, using steroids, drainage time and operative time are the current risk factors for non-superficial SSI following spinal surgery. In this study, operative time is the most important risk factor resulting in postoperative SSI.

Prediction of welding residual stresses (WRS) could be unreliable if mechanical property model of the material is inaccurate in finite element model. For TIG welding of 2219-T8 aluminum alloy, mechanical properties change with welding thermal process in a complex pattern. To build complete mechanical property model, influence of temperature, working hardening and “anneal” softening during welding must be considered. Gleeble and DIC system were used in this study to acquire tensile properties in different thermal process. Working hardening and “anneal” softening were studied by tensile curves. It was found that working hardening of 2219-T8 aluminum alloy would be eliminated at 200°C–300°C, which was called “anneal” softening. Then, fined mechanical property model was built for welding simulation. Results showed that “anneal” temperature had a great influence on longitudinal residual stress (LRS) in welding zone (WZ), partly melted zone (PMZ), over aged zone (OAZ) and part of heat affected zone (HAZ in this study) where maximum temperature was higher than 380°C. With correct consideration of “anneal” softening, LRS in these regions could be 100–150 MPa lower than that without “anneal” softening. Yield strength in these regions was greatly reduced due to “anneal” softening. However, for the regions with maximum temperature below 380°C, like other part of HAZ, “anneal” softening had little effect on WRS.

ABSTRACTAlthough highly infectious respiratory viral infections spread rapidly, humans have evolved a precise and complex immune mechanism to deal with respiratory viruses, with strong intrinsic, highly adaptive and specific humoral and cellular immunity. At the same time, vaccination against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is one of the most cost-effective and efficient means of preventing morbidity, severe illness, and death from Coronavirus disease 2019 (COVID-19). As the global epidemic of COVID-19 continues to evolve and vaccines are being developed, it is important to conduct studies on immunization strategies to optimize vaccination strategies when appropriate. This review was conducted to investigate the relationship between the immune response and the protective effect of different vaccination scenarios (including booster, sequential and hybrid immunity), and to provide a basis for the optimization of vaccination strategies and the development of new vaccines in the future.

BackgroundResearch has shown that stigmatization of professional psychological help-seeking is an important factor influencing attitudes toward seeking professional psychological help (ATSPPH). However, how perceived social support (PSS) and optimism have a mediating role is not clear.ObjectiveExamine the associations between ATSPPH, self-stigmatization of seeking help, PSS, and optimism in a cohort of Chinese high-school students (HSSs).MethodsAn offline survey was conducted in three high schools in Chongqing (China) from 20 February to 20 May 2023. Participants were HSSs recruited through their teachers. A total of 2,159 HSSs completed a survey on demographic information as well as the Self-Stigmatization of Seeking Help (SSOSH) score, ATSPPH, Perceived Social Support Scale (PASS), and Life Orientation Test (LOT). Mediation analyses were conducted using the “Process” macro in SPSS 26.0 to estimate the direct and indirect effects of self-stigmatization of seeking psychological help on ATSPPH.ResultsSelf-stigmatization of seeking psychological help was significantly and negatively related to ATSPPH among HSSs. Self-stigmatization of psychological help-seeking influenced ATSPPH through three pathways: (a) separate mediating effect of PSS (effect = −0.029); (b) separate mediating effect of optimism (effect = −0.069); (c) chain-mediating effect of PSS and optimism (effect = −0.017). These data suggested that self-stigmatization of psychological help-seeking could influence ATSPPH directly and indirectly through PSS and optimism.ConclusionPSS and optimism mediated the relationship between self-stigmatization of seeking help and ATSPPH. Improving the ability of HSSs to perceive social support and cultivating optimism could help improve the self-stigmatization of help-seeking and promote a positive attitude toward professional help-seeking.

Abstract Background As an important part of spinal fusion procedure, the selection of fusion cage size is closely related to the curative effect of the surgery. It mainly depends on the clinical experience of surgeons, and there is still a lack of objective standards. The purpose of this study is to propose the concept of relative intervertebral tension (RIT) for the first time and its grading standards to improve the surgical procedures of lumbar interbody fusion. Methods This retrospective study was conducted from January 2018 to July 2019. A total of 83 eligible patients including 45 men and 38 women with lumbar degenerative disease underwent transforaminal lumbar interbody fusion (TLIF) were included in this study. A total of 151 fusion segments were divided into group A, group B and group C according to the grading standards of RIT. In addition, parameters of intervertebral space angle (ISA), intervertebral space height (ISH), intervertebral space foramen (IFH), fusion rates, cage-related complications and cage heights were also compared among the three groups. Results The ISA in group A was the smallest among three groups in contrast with group C with largest ISA at the final follow-up(P 0.05), and a certain correlation between ISH and RIT was also observed. Conclusions The concept of RIT and the application of its clinical grading standards could simplify the surgical procedures of spinal fusion and reduce cage-related complications.

Abstract Progestin resistance is a problem for patients with endometrial carcinoma (EC) who require conservative treatment with progestin, and its underlying mechanisms remain unclear. YAP and TAZ (YAP/TAZ), downstream transcription coactivators of Hippo pathway, promote viability, metastasis and also drug resistance of malignant tumors. According to our microarray analysis, YAP/TAZ were upregulated in progestin resistant IshikawaPR cell versus progestin sensitive Ishikawa cell, which implied that YAP/TAZ may be a vital promotor of resistance to progestin. We found YAP/TAZ had higher expression levels among the resistant tissues than sensitive tissues. In addition, knocking down YAP/TAZ decreased cell viability, inhibited cell migration and invasion and increased the sensitivity of IshikawaPR cell to progestin. On the contrary, overexpression of YAP/TAZ increased cell proliferation, metastasis and promoted progestin resistance. We also confirmed YAP/TAZ were involved in progestin resistant process by regulating PI3K-Akt pathway. Furthermore, Verteporfin as an inhibitor of YAP/TAZ could increase sensitivity of IshikawaPR cells to progestin in vivo and in vitro. Our study for the first time indicated that YAP/TAZ play an important role in progestin resistance by regulating PI3K-Akt pathway in EC, which may provide ideas for clinical targeted therapy of progestin resistance.

Abstract Purpose This retrospective study was performed to analyze the clinical effects and complications of LSFCF in the surgical treatment of DLS combined with lumbar spinal stenosis (LSS). Methods A total of 26 eligible patients (mean age, 64.73 y; 17 men, 9 women) with DLS combined with LSS were included and LSFCF surgery was performed. An independent spine surgeon retrospectively reviewed the medical records and radiographs of all patients to evaluate surgical data and surgery-related complications. Preoperative, postoperative, and follow-up questionnaires were obtained to assess clinical outcomes. Results The average follow-up period of this study was 20.14 ± 5.21 months. The operation time and blood loss of patients underwent LSFCF were 129.33 ± 15.74 min and 356.13 ± 21.28 ml. The clinical effects of all patients in terms of visual analogue scale (VAS) and Oswestry disability index (ODI) have been significantly improved at the final follow-up postoperatively (P

Study Design: A retrospective study. Purpose: To firstly introduce a new surgical technique named as selective fenestration and axial decompression (SFAD) to improve the surgical treatment effects of lumbar degenerative diseases. Overview of literature: Posterior lumbar laminectomy and interbody fusion (LIF) is a standard surgical method for the treatment of lumbar degenerative diseases. However, its application has some limitations, and the incidence of spinal cord nerve injury is relatively high. Methods: This retrospective clinical study was conducted from January 2018 to February2020. A total of 101 eligible patients including 62 men and 39 women with lumbar disc herniation (LDH) or lumbar spinal stenosis (LSS) underwent LIF or SFAD were enrolled in this study. The average postoperative follow-up period of the LIF and SFAD groups were 20.1 ± 3.7 months and 19.7 ± 4.1 months, respectively. The clinical effects, imaging manifestation, and postoperative complications in LIF and SFAD groups were analyzed. Results: The operative time, blood loss and hospital stay in SFAD group were significantly lower than those in LIF group (P 0.05). Similarly, improvements of cobb angle of operated segment, intervertebral space height (ISH), intervertebral space foramen (IFH) and lumbar lordosis (LL) showed no statistical difference between the two groups (P > 0.05). Finally, the prevalence of complication rates in LIF group were significantly higher than the SFAD group during the follow-up period (P

Objective: Lumbar decompression surgery is an effective treatment for foot drop caused by LDD, but there is controversy about the prognostic factors affecting its efficacy. This study aimed to investigate the factors influencing the surgical outcome of foot drop due to LDD. Methods: A systematic database search of PubMed, Embase, Web of Science, Cochrane Library and Clinical Trials was performed for relevant articles published until May 2022. Two reviewers independently screened the literature, extracted data, and evaluated the quality of the studies based on the inclusion and exclusion criteria. The quality of the studies was evaluated using the Newcastle-Ottawa Scale (NOS), and STATA 16.0 software was used for meta-analysis. Results: A total of 730 relevant articles were initially identified and 9 articles were finally included in this study for data extraction and mea-analysis. The results of metaanalysis showed that patients with preoperative moderate muscle strength (2-3/5 on the Medical Research Council scale) had better prognosis compared to those with severe muscle weakness. Additionally, the presence of diabetes mellitus was associated with a poorer prognosis for patients with foot drop due to LDD. The OR values (95%CI) of these two factors were 5.882 (4.449, 7.776) and 5.657 (2.094,15.280) respectively. Conclusions: Patients with moderate muscle strength have a better prognosis compared to those with severe muscle weakness. The presence of diabetes mellitus is associated with a poorer prognosis for patients with foot drop due to LDD. These factors should be considered when predicting the surgical outcome of foot drop due to LDD.

Stable communication technologies in complex waters are a prerequisite for underwater operations. Underwater acoustic communication is susceptible to multipath interference, while underwater optical communication is susceptible to environmental impact. The underwater electric field communication established based on the weak electric fish perception mechanism is not susceptible to environmental interference, and the communication is stable. It is a new type of underwater communication technology. To address issues like short communication distances and high bit error rates in existing underwater electric field communication systems, this study focuses on underwater electric field communication systems based on direct sequence spread spectrum (DSSS) and binary phase shift keying (BPSK) modulation techniques. To verify the feasibility of the established spread spectrum electric field communication system, static communication experiments were carried out in a swimming pool using the DSSS-based system. The experimental results show that in fresh water with a conductivity of 739 μS/cm, the system can achieve underwater current electric field communication within a 11.2 m range with 10−6 bit errors. This paper validates the feasibility of DSSS BPSK in short-range underwater communication, and compact communication devices are expected to be deployed on underwater robots for underwater operations.

Voice coil motor (VCM) is a special direct drive linear motor, which can convert electric energy directly into mechanical energy without the use of transmission mechanism. VCM has the advantages of simple structure, good rigidity, fast response, silence, high linearity, no cogging force, no pulsation et al. It is widely used in the field of high-precision control. This paper reviews and summarizes the results of VCM research conducted by scholars from various countries, and summarizes the general situation of VCM servo control technology. Firstly, a basic description of VCM's mathematical model and common control mechanisms is provided. The benefits, drawbacks, and application of control techniques in the field of VCM are all explored in detail; At the same time, the methods to improve control strategy are proposed; Then, combined with the analysis and research of scholars in various countries on VCM, the problems of difficult to establish accurate model, friction disturbance and mechanical vibration of VCM and the solutions to the corresponding problems are summarized; Finally, a summary of VCM's application fields is provided.

Stroke is one of the important causes of both disability and death worldwide, which is very common in older adults. Post-stroke cognitive impairment (PSCI) is a common secondary damage of stroke, which is the main cause of long-term disability and decreased quality of life in stroke patients, which brings a heavy burden to society and families. Acupuncture, as one of the oldest and widely used worldwide techniques in Chinese medicine, is recommended by the World Health Organization (WHO) as an alternative and complementary strategy for improving stroke care. This review comprehensively summarizes literature from the last 25 years, showing that acupuncture can exert strong beneficial effect on PSCI. The mechanisms of acupuncture on PSCI involves anti-neuronal apoptosis, promoting synaptic plasticity, alleviating central and peripheral inflammatory reactions, and regulating brain energy metabolism disorders (including improving cerebral blood flow, glucose utilization and mitochondrial structure and function, etc.), etc. The effects and mechanisms of acupuncture on PSCI reviewed in this study provides scientific and reliable evidence for acupuncture application for PSCI.

ObjectivesThe mechanism of Brucella infection regulating macrophage phenotype has not been completely elucidated until now. This study aimed to determine the mechanism of Brucella abortus in the modulation of macrophage phenotype using RAW264.7 cells as a model.Materials and methodsRT-qPCR, ELISA and flow cytometry were used to detect the inflammatory factor production and phenotype conversion associated with M1/M2 polarization of macrophages by Brucella abortus infection. Western blot and immunofluorescence were used to analyze the role of nuclear factor kappa B (NF-κB) signaling pathway in regulation of Brucella abortus-induced macrophage polarization. Chromatin immunoprecipitation sequencing (Chip‐seq), bioinformatics analysis and luciferase reporter assay were used to screen and validate NF-κB target genes associated with macrophage polarization and further verify its function.ResultsThe results demonstrate that B. abortus induces a macrophage phenotypic switch and inflammatory response in a time-dependent manner. With the increase of infection time, B. abortus infection-induced M1-type increased first, peaked at 12 h, and then decreased, whereas the M2-type decreased first, trough at 12 h, and then increased. The trend of intracellular survival of B. abortus was consistent with that of M2 type. When NF-κB was inhibited, M1-type polarization was inhibited and M2-type was promoted, and the intracellular survival of B. abortus increased significantly. Chip‐seq and luciferase reporter assay results showed that NF-κB binds to the glutaminase gene (Gls). Gls expression was down-regulated when NF-κB was inhibited. Furthermore, when Gls was inhibited, M1-type polarization was inhibited and M2-type was promoted, the intracellular survival of B. abortus increased significantly. Our data further suggest that NF-κB and its key target gene Gls play an important role in controlling macrophage phenotypic transformation. ConclusionsTaken together, our study demonstrates that B. abortus infection can induce dynamic transformation of M1/M2 phenotype in macrophages. Highlighting NF-κB as a central pathway that regulates M1/M2 phenotypic transition. This is the first to elucidate the molecular mechanism of B. abortus regulation of macrophage phenotype switch and inflammatory response by regulating the key gene Gls, which is regulated by the transcription factor NF-κB.

Objective: Cage migration is a rare complication after lumbar fusion surgery, and it is also the cause of lumbar revision surgery. Previous studies have reported that many influencing factors can increase the incidence of cage migration. However, there still remains controversial. The current study was conducted to investigate the risk factors influencing incidence of cage migration. Methods: A systematic database search of PubMed, Embase, Web of Science, Cochrane Library and Clinical Trials was performed for relevant articles published until July 2022. According to the inclusion and exclusion criteria, two evaluators independently conducted literature screening, data extraction and quality evaluation of the obtained literature. The Newcastle–Ottawa Scale (NOS) score was used for quality evaluation, and meta-analysis was performed by STATA 16.0 software. Results: A total of 2126 relevant articles were initially identified, and 7 articles were finally included in this study for data extraction and meta-analysis. The results of meta-analysis showed that the bony endplate injury, pear-shaped disc, and screw loosening are significantly correlated with cage migration. The OR values (95%CI) of the three factors were 7.170 (3.015, 17.051), 8.056 (4.050, 16.023), and 12.840 (3.570, 46.177) respectively. Conclusion: Bony endplate injury, pear-shaped disc, and screw loosening are the current risk factors for cage migration postoperatively.

Abstract Objective Neuromyelitis optica spectrum disorders (NMOSDs) are blindness-causing neuritis; their pathogenesis is still not fully elucidated. Although it has been determined that Bruton tyrosine kinase (BTK) and NF-κB are associated with NMOSD, the changes that occur in different periods remain unknown. The study aimed to demonstrate the changes in the BTK/NF-κB pathway and related chemokines in different stages of NMOSDs. Methods A total of 32 patients with NMOSD were selected as the experimental group, and 32 healthy volunteers were included in the control group. In this study, the BTK/NF-κB pathway and related chemokines in the cerebrospinal fluid and peripheral blood samples of patients with NMOSD were analyzed in the acute or remission phase. Results BTK, NF-κB, PI3K, IKK, CXCL2, and CXCL12 levels in the NMOSD group in the acute or remission phase were significantly higher than those in the control group (p

Measurement(s) institutions, levels of government, dates and stringency of COVID-19 public health policy announcements Technology Type(s) content search of media and governement sources, expert coding and classification Factor Type(s) government office • government level • time in the pandemic • country • region Sample Characteristic - Organism government-announced COVID-19 public health policies Sample Characteristic - Environment regions, also aggregated to countries Sample Characteristic - Location global

Different dimensions of tensile test specimen can be used for qualification of welding procedure according to the existing standard. However, they are found to be dissimilar in tensile properties. In this study, the tensile properties of TIG welded 2219-T8 aluminum alloy joints were tested with different tensile specimen sizes. The welding residual stress releasing, specimen size effect, as well as fracture criteria were taken into account by using a sequential “welding - cutting - tensile” finite element simulating method. Results indicated that the degree of welding residual stress releasing decreased with the increasing of specimen width. For specimens with 250 mm width, over 80% of the residual stresses were kept. Tensile testing result showed that the tensile strength and elongation of joint decreased when the specimen width increased. The differences in tensile properties were proved to result from the size distinctions, rather than residual stress releasing, because the stress triaxialities were different in the specimens with different sizes. This phenomenon was precisely simulated by using the Johnson–Cook fracture criterion. Eventually, the constitutive equations of Johnson–Cook model were established based on the experimental tensile strengths to describe the tensile behaviors of 2219 aluminum alloy joints.

Flexible pressure sensors require functional materials accounting for mechanical compliance and electrical performance simultaneously but sensor materials often suffer from limited compressibility which decreases sensitivity over a large pressure range. Here, the authors demonstrate a heterophase ionogel with shape and stiffness memory for adaptive pressure sensing

The rise of omics research, spanning genomics, transcriptomics, proteomics, and epigenomics, has revolutionized our understanding of biological systems [...]

Abstract Alternative splicing (AS) events play a crucial role in the tumorigenesis and progression of cancer. Transcriptome data and Percent Spliced In (PSI) values of ovarian cancer patients were downloaded from TCGA database and TCGA SpliceSeq. Totally we identified 1472 AS events that were associated with survival of ovarian serous cystadenocarcinoma (OC) and exon skipping (ES) was the most important type. Univariate and multivariate Cox regression analysis were performed to identify survival-associated AS events and developed the prognostic model based on 11-AS events. The immune cells and different response to cytotoxic T lymphocyte associated antigen 4 (CTLA-4) and programmed cell death protein 1 (PD-1) blockers in low-risk and high-risk group of OC patients were analyzed. Ten kinds of immune cells were found up-regulated in low-risk group. Activated B cell, natural killer T cell, natural killer cell and regulatory T cell were associated with survival of OC. The patients in low-risk group had good response to CTLA-4 and PD-1 blockers treatment. Moreover, a regulatory network was established according to the correlation between AS events and splicing factors (SFs). The present study provided valuable insights into the underlying mechanisms of OC. AS events that were correlated with the immune system might be potential therapeutic targets.

Inflammatory bowel disease (IBD) is a chronic recurrent intestinal disease. The incidence rate of IBD is increasing year by year, which seriously endangers human health worldwide. More and more studies have shown that medicinal plants or their main phytochemicals have great potential in the treatment of intestinal diseases. However, the disadvantages of low oral absorption rate, low biological distribution and low systemic bioavailability limit their clinical application to a certain extent. In recent years, the application of nanotechnology has made it possible to treat IBD. Nanoparticles (NPs) drug delivery system has attracted special attention in the treatment of IBD due to its small size, low immunogenicity, surface modification diversity, targeting and other advantages. Synthetic nanoparticles and extracellular vehicles (EVs) can deliver drug components to colon, and play a role in anti-inflammation, regulation of oxidative stress, improvement of intestinal flora, etc. In addition, some medicinal plants can secrete EVs by themselves, and carry biological molecules with therapeutic effects to act on the intestine. Some clinical trials to evaluate the safety, tolerance, toxicity and effectiveness of EVs-loaded drugs in IBD are also progressing steadily. This review introduces that synthetic nanoparticles and medicinal plants derived EVs can play an important role in the treatment of IBD by carrying the effective active phytochemicals of medicinal plants, and discuss the limitations of current research and future research needs, providing a scientific and reliable basis and perspective for further clinical application and promotion.

People's emotions may be affected by the sound environment in court. A courtroom's sound environment usually consists of the people's voices, such as the judge's voice, the plaintiff's voice, and the defendant's voice. The judge, plaintiff, and defendant usually express their emotions through their voices. Human communication is heavily reliant on emotions. Emotions may also reflect a person's condition. Therefore, People's emotions at the Court must be recognized, especially for vulnerable groups, and the impact of the sound on the defendant's motions and judgment must be inferred. However, people's emotions are difficult to recognize in a courtroom. In addition, as far as we know, no existing study deals with the impact of sound on people in court. Based on sound perception, we develop a deep neural network-based model to infer people's emotions in our previous work. In the proposed model, we use the convolutional neural network and long short-term memory network to obtain features from speech signals and apply a dense neural network to infer people's emotions. Applying the model for emotion prediction based on sound at court, we explore the impact of sound at court on the defendant. Using the voice data collected from fifty trail records, we demonstrate that the voice of the judge can affect the defendant's emotions. Angry, neutrality and fear are the top three emotions of the defendant in court. In particular, the judge's voice expressing anger usually induces fear in the defendant. The plaintiff's angry voice may not have a substantial impact on the defendant's emotions.

BackgroundTotal pancreatectomy (TP) has been increasingly performed in recent years. However, studies on diabetes management after TP during different postoperative periods are still limited.ObjectivesThis study aimed to evaluate the glycemic control and insulin therapy of patients undergoing TP during the perioperative and long-term follow-up period.MethodsNinety-three patients undergoing TP for diffuse pancreatic tumors from a single center in China were included. Based on preoperative glycemic status, patients were divided into three groups: nondiabetic group (NDG, n = 41), short-duration diabetic group (SDG, preoperative diabetes duration ≤12 months, n = 22), and long-duration diabetic group (LDG, preoperative diabetes duration >12 months, n = 30). Perioperative and long-term follow-up data, including the survival rate, glycemic control, and insulin regimens, were evaluated. Comparative analysis with complete insulin-deficient type 1 diabetes mellitus (T1DM) was conducted.ResultsDuring hospitalization after TP, glucose values within the target (4.4-10.0 mmol/L) accounted for 43.3% of the total data, and 45.2% of the patients experienced hypoglycemic events. Patients received continuous intravenous insulin infusion during parenteral nutrition at a daily insulin dose of 1.20 ± 0.47 units/kg/day. In the long-term follow-up period, glycosylated hemoglobin A1c levels of 7.43 ± 0.76% in patients following TP, as well as time in range and coefficient of variation assessed by continuous glucose monitoring, were similar to those in patients with T1DM. However, patients after TP had lower daily insulin dose (0.49 ± 0.19 vs 0.65 ± 0.19 units/kg/day, P < 0.001) and basal insulin percentage (39.4 ± 16.5 vs 43.9 ± 9.9%, P = 0.035) than patients with T1DM, so did those using insulin pump therapy. Whether in the perioperative or long-term follow-up period, daily insulin dose was significantly higher in LDG patients than in NDG and SDG patients.ConclusionsInsulin dose in patients undergoing TP varied according to different postoperative periods. During long-term follow-up, glycemic control and variability following TP were comparable to complete insulin-deficient T1DM but with fewer insulin needs. Preoperative glycemic status should be evaluated as it could guide insulin therapy after TP.

In order to explore the spatial layout of fish assemblages and their related environmental factors, a survey of species composition was conducted in the Anning River. From 2018 to 2021, 28 sites at 987–2,077 m in altitude were surveyed, gathering 64 species in total, of which 60 were native and 4 were exotic. Fifteen of the species sampled were indigenous to the upper Yangtze River, and two were classified as critically endangered or endangered. Fish communities displayed significant upstream-to-downstream variation. There are two site-groups, one covering upstream and tributaries and the other covering midstream and downstream. The upstream-tributary fish assemblages had a dominance of Nemacheilidae and Schizothoracinae, which are plateau species with habitat preferences for boulders and fast-flowing water. Conversely, the mid-downstream fish assemblages had a dominance of Gobioninae, Cyprininae, Bagridae and Siluridae, which are common river species that prefer slow-flowing water. The spatial patterns of these fish communities were chiefly affected by altitude and substratum composition. The fish assemblage pattern was consistent with the zonation concept because the fish communities changed abruptly and clearly along the upstream–downstream axis. The findings of this study offer a crucial research foundation for comprehending the fish assemblages of mountainous rivers, which will be conducive to biodiversity management and habitat preservation of aquatic species.

Breast cancer has now become the most commonly diagnosed cancer worldwide. It is a highly complex and heterogeneous disease that comprises distinct histological features and treatment response. With the development of molecular biology and immunology, immunotherapy has become a new field of breast cancer treatment. Identifying cell-type-specific genes critical to the immune microenvironment contributes to breast cancer treatment. Single-cell RNA sequencing (scRNA-seq) technology could serve as a powerful tool to analyze cellular genetic information at single-cell resolution and to uncover the gene expression status of each cell, thus allowing comprehensive assessment of intercellular heterogeneity. Because of the influence of sample size and sequencing depth, the specificity of genes in different cell types for breast cancer cannot be fully revealed. Therefore, the present study integrated two public breast cancer scRNA-seq datasets aiming to investigate the functions of different type of immune cells in tumor microenvironment. We identified total five significant differential expressed genes of B cells, T cells and macrophage and explored their functions and immune mechanisms in breast cancer. Finally, we performed functional annotation analyses using the top fifteen differentially expressed genes in each immune cell type to discover the immune-related pathways and gene ontology (GO) terms.

Partially melted zone (PMZ) is generally the weak region in 2219-T8 aluminum alloy TIG welding joint. In this work, a microstructure-based numerical simulation was performed to analyze the tensile behavior of PMZ. The constitutive behavior and damage mechanism of eutectic structure and Al matrix were determined. Stress–strain distribution and fracture behavior in tensile process were analyzed. Quantitative relationships between eutectic structure and tensile properties of PMZ were proposed. The results showed that the maximum area and clustering of eutectic particles have significant effects on the tensile strength and elongation. Plastic strain mainly concentrated in matrix, and stress concentration occurred in larger eutectic. The tensile cracks initiated from eutectic structure and then propagated into matrix and resulted in the final fracture.

Vaccination is the most effective means of protecting people from influenza virus infection. The effectiveness of existing vaccines is very limited due to antigenic drift of the influenza virus. Therefore, there is a requirement to develop a universal vaccine that provides broad and long-lasting protection against influenza. CD8+ T-cell response played a vital role in controlling influenza virus infection, reducing viral load, and less clinical syndrome. In this study, we optimized the HA sequences of human seasonal influenza viruses (H1N1, H3N2, Victoria, and Yamagata) by designing multivalent vaccine antigen sets using a mosaic vaccine design strategy and genetic algorithms, and designed an HA mosaic cocktail containing the most potential CTL epitopes of seasonal influenza viruses. We then tested the recombinant mosaic antigen, which has a significant number of potential T-cell epitopes. Results from genetic evolutionary analyses and 3D structural simulations demonstrated its potential to be an effective immunogen. In addition, we have modified an existing neutralizing antibody-based seasonal influenza virus vaccine to include a component that activates cross-protective T cells, which would provide an attractive strategy for improving human protection against seasonal influenza virus drift and mutation and provide an idea for the development of a rationally designed influenza vaccine targeting T lymphocyte immunity.

This paper tested the ability of Mandarin learners of German, whose native language has lexical tone, to imitate pitch accent contrasts in German, an intonation language. In intonation languages, pitch accents do not convey lexical information; also, pitch accents are sparser than lexical tones as they only associate with prominent words in the utterance. We compared two kinds of German pitch-accent contrasts: (1) a “non-merger” contrast, which Mandarin listeners perceive as different and (2) a “merger” contrast, which sounds more similar to Mandarin listeners. Speakers of a tone language are generally very sensitive to pitch. Hypothesis 1 (H1) therefore stated that Mandarin learners produce the two kinds of contrasts similarly to native German speakers. However, the documented sensitivity to tonal contrasts, at the expense of processing phrase-level intonational contrasts, may generally hinder target-like production of intonational pitch accents in the L2 (Hypothesis 2, H2). Finally, cross-linguistic influence (CLI) predicts a difference in the realization of these two contrasts as well as improvement with higher proficiency (Hypothesis 3, H3). We used a delayed imitation paradigm, which is well-suited for assessing L2-phonetics and -phonology because it does not necessitate access to intonational meaning. We investigated the imitation of three kinds of accents, which were associated with the sentence-final noun in short wh-questions (e.g., Wer malt denn Mandalas, lit: “Who draws PRT mandalas?” “Who likes drawing mandalas?”). In Experiment 1, 28 native speakers of Mandarin participated (14 low- and 14 high-proficient). The learners’ productions of the two kinds of contrasts were analyzed using General Additive Mixed Models to evaluate differences in pitch accent contrasts over time, in comparison to the productions of native German participants from an earlier study in our lab. Results showed a more pronounced realization of the non-merger contrast compared to German natives and a less distinct realization of the merger contrast, with beneficial effects of proficiency, lending support to H3. Experiment 2 tested low-proficient Italian learners of German (whose L1 is an intonation language) to contextualize the Mandarin data and further investigate CLI. Italian learners realized the non-merger contrast more target-like than Mandarin learners, lending additional support to CLI (H3).

Brucella can inhabit hostile environments, including osmotic stress. How Brucella responds collectively to osmotic stress is largely unexplored, particularly in spatially structured communities such as a biofilm. To gain insight into this growth mode, we set out to characterize the Brucella melitensis 16M biofilm, describe its phenotype, and carry out a comparative transcriptomic analysis between biofilms under osmotic stress and control conditions. We determined that the bacteria challenged with 1.5 M NaCl had a reduced ability to aggregate and form clumps and develop a biofilm; however, the salt stress promoted the release of the outer membrane vesicles from the biofilm. Together with the genotypical response to osmotic stress, we identified 279 differentially expressed genes in B. melitensis 16M grown under osmotic conditions compared with control conditions; 69 genes were upregulated and 210 downregulated. Under osmotic stress, the main changed genes of biofilm were predicted to be involved in flagellar assembly, cell envelope, translation, small RNA regulation, transport and binding proteins, and energy metabolism. In addition, the ABC transporter was enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. We highlight 12 essential ABC transporter genes associated with a bacterial response to osmotic stress at the biofilm stage, including one specific locus, BME_RS12880, mediating betaine accumulation in biofilms to eliminate osmotic stress. The current study results can help researchers gain insights into B. melitensis 16M biofilm adaptation to osmotic stress and provide information for developing intervention strategies to control Brucella.

Despite the recognized epidemiological importance of ticks as vectors for pathogens that cause numerous zoonotic and veterinary diseases, data regarding the pathogens of pet dogs and their parasitic ticks in the Junggar Basin are scarce. In this study, a total of 178 blood samples and 436 parasitic ticks were collected from pet dogs in Junggar Basin, Xinjiang Uygur Autonomous Region (XUAR), north-western China. All ticks were identified as Rhipicephalus turanicus sensu stricto (s.s.) according to morphological and molecular characteristics. Rh. turanicus s.s. ticks were collected from pet dogs in China for the first time. Seven tick-borne pathogens, such as Ehrlichia chaffeensis, Anaplasma phagocytophilum, Rickettsia massiliae, Candidatus R. barbariae, Brucella spp., Rickettsia sibirica, and Anaplasma ovis, were detected from ticks, whereas the first five bacteria were detected from blood samples of dogs. Brucella spp. was the most predominant pathogen in both blood samples and ticks of pet dogs, with the detection rates of 16.29 and 16.74%, respectively. Moreover, 17 ticks and 1 blood sample were co-infected with two pathogens, and 1 tick was co-infected with three pathogens. This study provided molecular evidence for the occurrence of Anaplasma spp., Ehrlichia spp., Rickettsia spp., and Brucella spp. circulating in pet dogs and their parasitic ticks in Junggar Basin, north-western China. These findings extend our knowledge of the tick-borne pathogens in pet dogs and their parasitic ticks in Central Asia; therefore, further research on these pathogens and their role in human and animal diseases is required.

Abstract Water flow rate plays an important role in the modeling prediction, fault detection and diagnosis, and performance optimization of the variable water flow air‐conditioning (VWFAC) system. However, flowmeters employed by the system's terminals have not been widely used in engineering applications for the constraints in installation space and high installation and retrofit costs. Therefore, in this study, a terminal flow rate estimation method is proposed for the VWFAC system to reduce the dependency on flowmeters. The water flow rate estimation model is developed based on the flow resistance characteristics inside the air handling unit (AHU) and was trained and verified at the Monitoring and Control Laboratory established in the Dalian University of Technology. The results indicate that the maximum root‐mean‐square error (RMSE) during the training and validation sessions are 0.038 m3/h and 0.028 m3/h, respectively, while the corresponding mean absolute percentage error (MAPE) are below 1.4% and 6.5%, which is acceptable for engineering applications. To improve the flow rate estimation accuracy of the model, water temperature, water flow rate, and pressure difference are suggested to cover a wide varied range during the training session as much as possible. Systematic error is an important index in determining the demands for sensor's accuracy class. According to the results of water flow rate, the systematic errors of the estimates are ranged between 0.51% and 0.76%, only about 1/4 to 1/3 of the measurements systematic errors. Based on the error propagation theory, the water flow rate estimates would be reliable if the systematic error of the pressure measurements does not over twice that of the water flow rate measurements. This flow rate estimation method can be further applied to other thermal engineering systems to bring considerable economic benefits for engineering applications.

Abstract Objectives The study of sex differences in hyperuricemia can provide not only a theoretical basis for this clinical phenomenon but also new therapeutic targets for urate-lowering therapy. In the current study, we aimed to confirm that estradiol can promote intestinal ATP binding cassette subfamily G member 2 (ABCG2) expression to increase urate excretion through the PI3K/Akt pathway. Methods The estradiol levels of hyperuricemia/gout patients and healthy controls were compared, and a hyperuricemia mouse model was used to observe the urate-lowering effect of estradiol and the changes in ABCG2 expression in the kidney and intestine. In vivo and in vitro intestinal urate transport models were established to verify the urate transport function regulated by estradiol. The molecular pathway by which estradiol regulates ABCG2 expression in intestinal cells was explored. Results The estradiol level of hyperuricemia/gout patients was significantly lower than that of healthy controls. Administering estradiol benzoate (EB) to both male hyperuricemic mice and female mice after removing the ovaries confirmed the urate-lowering effect of estradiol, and hyperuricemia and estradiol upregulated the expression of intestinal ABCG2. Estradiol has been confirmed to promote urate transport by upregulating ABCG2 expression in intestinal urate excretion models in vivo and in vitro. Estradiol regulates the expression of intestinal ABCG2 through the PI3K/Akt pathway. Conclusion Our study revealed that estradiol regulates intestinal ABCG2 through the PI3K/Akt pathway to promote urate excretion, thereby reducing serum urate levels.

In most cases the frequency range of a damping material is adapted to a specific application. Huang et al. design a gel filled with a polymeric fluid that bypasses this problem and offers an unusually broad window over which vibrational energy is effectively dissipated.