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The concept of sustainable development as a consensus is being recognized and practiced by the international community, and how to promote China–ASEAN trade relations and stable and sustainable economic development is an important guarantee for the coordinated economic development of the region. This paper studies the trade relationship between China and the ten ASEAN countries by using the theory of ecological population evolution and determines the ecological trade relationship between China and the ten ASEAN countries by establishing the Lotka–Volterra model of the evolution of two populations—the relationship of competition, the relationship of partial benefit, and the relationship of mutually beneficial symbiosis—so as to analyze the degree of trade friction between the two sides and propose corresponding strategies for coordinated development of the regional economy. According to the results of the population evolution competition model, there is a competitive relationship between China, Brunei, and Vietnam, which may cause trade friction. China has a trade-biased relationship with Indonesia, Cambodia, Malaysia, and Singapore, which is less likely to cause trade friction. China’s trade with Laos, Myanmar, the Philippines, and Thailand is mutually beneficial. The possibility of trade friction is low, and the trade relationship between China and ASEAN as a whole is also mutually beneficial. On this basis, in order to further enhance the trade relations between PRC and the ten ASEAN countries and promote the coordinated development of the regional economy, this paper puts forward the policy suggestions of improving infrastructure, strengthening independent innovation, seeking cooperation areas, and actively investing in foreign direct investment.

DNA hypermethylation and the silencing of tumor suppressor genes caused by DNA hypermethylation is considered as a molecular hallmark of many kinds of cancers. Procaine, a local anesthetic, has been shown as a potential DNA methylation inhibitor in some types of cancers. However, the influence of procaine on DNA methylation regulation as well as the biological function in gastric cancer is still unknown. We report here that procaine represses the DNA-methylation level and promotes the proliferation arrest and apoptosis of gastric cancer cells. Global DNA methylation measurement demonstrates that procaine significantly reduces the global DNA methylation level. Analyses of the DNMTs expression and activity show procaine represses the activity, but not the expression, of DNMT1/DNMT3A. Further evidence on specific genes shows that procaine reduces the DNA methylation level in the promoter regions of CDKN2A and RARβ genes through abrogating the binding of DNMT1/DNMT3A toward these regions. This repression would not be reversed by the overexpression of DNMT1/DNMT3A. Moreover, RT-qPCR and luciferase report assays demonstrate that procaine leads to the upregulation of CDKN2A and RARβ due to the activation of the promoter of these genes. In the end, we test the function of procaine toward gastric cancer cells and find that procaine has the growth inhibitory and apoptosis inducement effect toward gastric cancer cells. Collectively, our data not only uncovers the regulation mechanisms of procaine to DNA methylation but also suggests an anti-tumor potential of procaine specific to the gastric carcinoma and provides a new therapeutic strategy for gastric carcinoma.

Objective To investigate the ideal dosage of dexmedetomidine(DEX) with 1.0 μg/kg fentanyl for monitored anesthesia care (MAC)during endoscopic variceal ligation (EVL). Methods A total of 60 patients, of ASA physical status 2-3, aged 36–59 yr, with body weight 50–75 kg, scheduled for elective EVL, were randomly divided into 3 groups (n=20): dexmedetomidine 1.0, 1.5 and 2.0 μg/kg groups (D1, D2 and D3 groups. After fentanyl 1.0 μg/kg was infused intravenously, the loading dosage of DEX 1.0, 1.5, 2.0μg/kg was separately continuous infused in 10 min. When the modified OAA / S scale > 3 points, EVL was carried out. The modified OAA/S score at the time-points of before induction (T0), before endoscope insertion (T1) and 5mins later(T2), end of surgery (T3) were recorded. The operation duration, recovery time, satisfaction of patient and doctor, incidence of nausea, body movement, bradycardia, hypotension, tachycardia, hypertension and hypoxemia was recorded. Results There were no differences in the 3 groups about the general status, operation duration and satisfaction score(P>0.05). (1) Before endoscope insertion(T1),the improved OAA/S score in Group D3 (4.4±0.2) were higher than D1(3.4±0.5) and D2 groups(3.8±0.3) (P>0.05).At the time-point of 5mins later(T2), the score in Group D3 (4.5±0.3)were higher than D1(3.5±0.6)and D2 groups(3.7±0.4) (P 0.05). At the end of surgery (T3),the score were almost similar(P>0.05)(2) Compared with group D1 (3.1±0.9)and D2(3.8±0.8), group D3 (6.6±1.2) had longer recovery time(min) (P 0.05). The incidences of bradycardia (40%) in group D3 is significantly higher than group D1(0) and D2(10%) (P 0.05).Conclusion Combined with 1.0μg/kg fentanyl, 1.5 μg/kg DEX is more efficacy and safer for EVL in the status of monitored anesthesia care.

By using seeding-box total fertilization technology, a two-year field plot experiment was conducted to study the effects of applying medium rate of controlled-release urea fertilizer (MN, 80 kg N x hm(-2)), high rate of controlled-release urea fertilizer (HN, 120 kg N x hm(-2)), and conventional urea fertilizer (FP, 300 kg N x hm(-2)) on rice yield and nitrogen loss. As compared with FP, HN did not decrease rice yield significantly, and MN and HN increased the two-year average nitrogen use efficiency (NUE) by 26.2% and 20.7%, respectively (the NUE in treatment FP was 33.2%). In treatment FP, the total N concentration in surface water peaked after 1-3 days of urea application; while in treatments MN and HN, the total N concentration in surfate water peaked after 7-9 days of urea application, and was significantly lower than that in treatment FP throughout the rice growth period. The nitrogen leaching loss in treatment FP mainly occurred at tillering stage, while that in treatments MN and HN delayed to tillering-flowering stage. In all treatments, the NO3(-)-N loss accounted for 59.7% - 64.2% of the total N loss. HN decreased the total N leaching loss by 51.8%, as compared with FP.