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From the deep-sea-derived fungus Aspergillus candidus, one novel (1) and three known (2–4) p-terphenyl derivates were isolated. The structure of the new compound was established mainly on the basis of extensive analysis of 1D and 2D NMR data. All four isolates were tested for in vitro anti-food allergic and antitumor bioactivities. Compounds 3 and 4 showed potent antiproliferative effect against four cancer cells of Hela, Eca-109, Bel-7402, and PANC-1 with IC50 values ranging from 5.5 μM to 9.4 μM. [ABSTRACT FROM AUTHOR]

Background: The expression patterns and prognostic value of Procollagen-lysine, 2-oxoglutarate 5-dioxygenase (PLOD) family genes in breast cancer remain to be elucidated., Methods: The expression levels, prognostic value, and biological function of PLODs were determined using Oncomine, cBioPortal, GEPIA, Timer, UALCAN, PrognoScan, GeneMANIA, Metascape, and breast cancer tissue microarrays., Results: The expressions of PLOD1 and PLOD3 were upregulated in breast cancer tissues, indicating worse clinical stages. High expression levels of PLOD family genes were associated with worse disease-free survival and distant metastasis-free survival, while high expression levels of PLOD1 and PLOD3 were related to worse overall survival in all breast cancer patients. The levels of PLOD family genes were all significantly higher in the age ≤51 y group, HR-negative patients, and triple negative breast cancer (TNBC) patients. They are associated with tumor-infiltrating immune cells (TIICs), including CD4+ T cells, CD8+ T cells, B cells, macrophages, neutrophils, and dendritic cells. According to co-expression gene analysis and functional enrichment, they are associated with protein hydroxylation, collagen biosynthesis and modifying enzymes, collagen metabolism, RNA splicing, extracellular matrix organization, VEGFA-VEGFR2 signaling pathway, and skeletal system development. Immunohistochemistry showed that the expressions of all PLOD family genes were significantly elevated in breast cancer tissues. PLOD1 expression was positively correlated with ER, TNBC status, and tumor grade. PLOD2 expression was positively connected with Ki-67 status. PLOD3 expression was positively related with age and tumor grade., Conclusions: PLOD family genes are novel potential prognostic biomarkers for breast cancer, and targeting PLOD inhibitors might be an effective strategy for breast cancer therapy.

Concentrations of PAHs in leaves of six dominant species in the Pearl River Delta of South China were determined with gas chromatography and mass spectrometry (GC-MS), and synchronously leaf-lipid contents, leaf areas, specific leaf areas (SLA) were measured. The characters of PAHs abundance and influence factors on them were investigated. The results show that concentrations of PAHs in pine needles are higher than those in broad-leaves remarkably, and the highest value (1034.7 ng x g(-1) in Pinus massoniana) is fivefold of the lowest (199.7 ng x g(-1) in Schima superba) . Flu, Phe, Fluo, Pyr, Chr are the main components of sigma PAHs, and the concentrations of 3-ring and 4-ring PAHs exceed 80% of the total PAHs. Significant positive correlations between the components and sigma PAHs were also detected. The PAHs contents by per lipid could reduce inter-species variance obviously, and the diversity is no more than two times (6.8 ng x mg(-1) and 2.7 ng x mg(-1), respectively). Adversely, the PAHs contents by per cm2 magnify inter-species variance, and the highest value (15.3 ng x cm(-2)) ascends to eight times of the lowest (1.9 ng x cm(-2)). Furthermore, significant positive correlation between PAHs and leaf-lipid contents was identified, as well as negative correlation between PAHs and specific leaf areas. All of these indicate that leaf-lipid contents and specific leaf areas are two significant factors which influenced PAHs contents in leaves.