263 results on '"Thurgood P"'
Search Results
2. Therapeutic errors involving diabetes medications reported to United States poison centers
- Author
-
Ashley Thurgood Giarman, Hannah L. Hays, Jaahnavi Badeti, Natalie I. Rine, Henry A. Spiller, Motao Zhu, and Gary A. Smith
- Subjects
Therapeutic error ,Adverse event ,National Poison Data System ,Diabetes ,Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background To investigate the characteristics and trends of therapeutic errors that occur outside of healthcare facilities involving diabetes medications reported to US poison centers. Methods National Poison Data System data from 2000 to 2021 were retrospectively analyzed. Results There were 157,623 exposure cases of non-healthcare facility-related therapeutic errors associated with diabetes medications as the primary substance reported to US poison centers from 2000 to 2021. The rate of all therapeutic errors involving diabetes medications increased by 279.8% from 2000 to 2011, followed by a slower 15.0% increase to 2021. Half (50.1%) of the exposure cases were treated/evaluated and released and 44.1% did not receive treatment in a healthcare facility; however, 9.9% experienced a serious medical outcome, including 17 fatalities, and 1.0% were admitted to a critical care unit and 2.2% to a non-critical care unit. Insulin had the highest rates of therapeutic errors and serious medical outcomes, while sulfonylureas and insulin had the highest medical hospital admission rates. Metformin accounted for 59% (n = 10) of fatalities. Conclusions Although most cases of therapeutic errors involving diabetes medications had no or minimal clinical consequences, an important minority were associated with a serious medical outcome or medical hospital admission. Increased efforts to prevent therapeutic errors involving diabetes medications are warranted.
- Published
- 2024
- Full Text
- View/download PDF
3. Uptake and Economic Value of Macro- and Micronutrient Minerals in Wheat Residue
- Author
-
Curtis B. Adams, Christopher W. Rogers, Juliet M. Marshall, Patrick Hatzenbuehler, Olga S. Walsh, Garrett Thurgood, Biswanath Dari, Grant Loomis, and David D. Tarkalson
- Subjects
fertilizer ,nutrients ,small grains ,straw ,residue ,wheat ,Agriculture - Abstract
Wheat (Triticum aestivum, L.) producers have the choice to retain or remove residue from the cropping system following grain harvest. In the U.S. Pacific Northwest and other regions, wheat residue is often sold to increase operational profitability, especially from higher-yielding systems. But there are several benefits to retaining residue, including recycling of mineral nutrients contained therein, though this is understudied. Therefore, the primary objectives of this research were to collect and analyze a large and diverse dataset on wheat residue nutrient uptake (N, P, K, Ca, Mg, S, Fe, Zn, Mn, Cu), develop tools to estimate nutrient amounts in residue, and make economic estimates of the fertilizer replacement value of those nutrients. This was accomplished by conducting replicated variety trials on five classes of wheat across many Pacific Northwest sites over two years, then collecting and analyzing data on wheat residue biomass, residue nutrient concentrations, and grain yield. The results showed that wheat residue contained a significant amount of nutrients, but was particularly concentrated in K. Production environment had the most substantial effect on residue mineral uptake amounts, due to site differences in yield and soil nutrient availability. To enable simple estimation of residue nutrient uptake across a broad range of wheat production levels, two estimation tools are presented herein. Economic analysis showed the substantial monetary value of residual nutrients. For example, in a high-yielding wheat crop (9 Mg ha−1), the average fertilizer replacement value of just residue N, P, K, and S was similar to the entire fertilizer budget to grow the crop (~$211 vs. $205 ha−1), not considering micronutrients in the residue or any nutrients removed through grain harvest. In making residue management decisions, wheat producers should consider the tradeoff between the immediate economic gains of residue sale and the multifaceted benefits of residue retention, including savings on future nutrient costs.
- Published
- 2024
- Full Text
- View/download PDF
4. Investigating the effects of low intensity visible light on human keratinocytes using a customized LED exposure system
- Author
-
Emily Sutterby, Chanly Chheang, Peter Thurgood, Khashayar Khoshmanesh, Sara Baratchi, and Elena Pirogova
- Subjects
Medicine ,Science - Abstract
Abstract Photobiomodulation (PBM) refers to the use of light to modulate cellular processes, and has demonstrated utility in improving wound healing outcomes, and reducing pain and inflammation. Despite the potential benefits of PBM, the precise molecular mechanisms through which it influences cell behavior are not yet well understood. Inconsistent reporting of key light parameters has created uncertainty around optimal exposure profiles. In addition, very low intensities of light,
- Published
- 2022
- Full Text
- View/download PDF
5. On the periodicity of linear and nonlinear oscillatory reconnection
- Author
-
Thurgood, J. O., Pontin, D. I., and McLaughlin, J. A.
- Subjects
Astrophysics - Solar and Stellar Astrophysics ,Physics - Plasma Physics - Abstract
(Abridged for ArXiv) An injection of energy towards a magnetic null point can drive reversals of current sheet polarity leading to time-dependent Oscillatory Reconnection, which is a possible explanation of how periodic phenomena can be generated when reconnection occurs in the solar atmosphere. However, the details of what controls the period of these oscillations is poorly understood, despite being crucial in assessing whether OR can account for observed periodic behaviour. This paper aims to highlight that different types of reconnection reversal are supported about null points, and that these are distinct from the oscillation on the closed-boundary, linear systems considered in the 1990s. In particular, we explore the features of a nonlinear oscillation local to the null point, and examine the effect of resistivity and perturbation energy on the period, contrasting it to the linear case. It is found that in the linear systems, the inverse Lundquist number dictates the period, provided the perturbation energy is small relative to the inverse Lundquist number defined on the boundary, regardless of the broadband structure of the initial perturbation. However, when the perturbation energy exceeds the threshold required for 'nonlinear' null collapse to occur, a complex oscillation of the magnetic field is produced which is, at best, only weakly-dependent on the resistivity. The resultant periodicity is strongly influenced by the amount of free energy, with more energetic perturbations producing higher-frequency oscillations. Crucially, with regards to typical solar-based and astrophysical-based input energies, we demonstrate that the majority far exceed the threshold for nonlinearity to develop. This substantially alters the properties and periodicity of both null collapse and subsequent OR. Therefore, nonlinear regimes of OR should be considered in solar and astrophysical contexts., Comment: Accepted in A&A, 5 Figures (1 animated)
- Published
- 2018
- Full Text
- View/download PDF
6. Resistively-limited current sheet implosions in planar anti-parallel (1D) and null-point containing (2D) magnetic field geometries
- Author
-
Thurgood, J. O., Pontin, D. I., and McLaughlin, J. A.
- Subjects
Physics - Plasma Physics ,Astrophysics - Solar and Stellar Astrophysics ,Physics - Fluid Dynamics - Abstract
Implosive formation of current sheets is a fundamental plasma process. Previous studies focused on the early time evolution, while here our primary aim is to explore the longer-term evolution, which may be critical for determining the efficiency of energy release. To address this problem we investigate two closely-related problems, namely: (i) 1D, pinched anti-parallel magnetic fields and (ii) 2D, null point containing fields which are locally imbalanced ('null-collapse' or 'X-point collapse'). Within the framework of resistive MHD, we simulate the full nonlinear evolution through three distinct phases: the initial implosion, its eventual halting mechanism, and subsequent evolution post-halting. In a parameter study, we find the scaling with resistivity of current sheet properties at the halting time is in good agreement - in both geometries - with that inferred from a known 1D similarity solution. We find that the halting of the implosions occurs rapidly after reaching the diffusion scale by sudden Ohmic heating of the dense plasma within the current sheet, which provides a pressure gradient sufficient to oppose further collapse and decelerate the converging flow. This back-pressure grows to exceed that required for force balance and so the post-implosion evolution is characterised by the consequences of the current sheet `bouncing' outwards. These are: (i) the launching of propagating fast MHD waves (shocks) outwards and (ii) the width-wise expansion of the current sheet itself. The expansion is only observed to stall in the 2D case, where the pressurisation is relieved by outflow in the reconnection jets. In the 2D case, we quantify the maximum amount of current sheet expansion as it scales with resistivity, and analyse the structure of the reconnection region which forms post-expansion, replete with Petschek-type slow shocks and fast termination shocks., Comment: Published open access in Physics of Plasmas - go to main journal for animations https://doi.org/10.1063/1.5035489
- Published
- 2018
- Full Text
- View/download PDF
7. Implosive collapse about magnetic null points: A quantitative comparison between 2D and 3D nulls
- Author
-
Thurgood, J. O., Pontin, D. I., and McLaughlin, J. A.
- Subjects
Astrophysics - Solar and Stellar Astrophysics ,Physics - Fluid Dynamics ,Physics - Plasma Physics ,Physics - Space Physics - Abstract
Null collapse is an implosive process whereby MHD waves focus their energy in the vicinity of a null point, forming a current sheet and initiating magnetic reconnection. We consider, for the first time, the case of collapsing 3D magnetic null points in nonlinear, resistive MHD using numerical simulation, exploring key physical aspects of the system as well as performing a detailed parameter study. We find that within a particular plane containing the 3D null, the plasma and current density enhancements resulting from the collapse are quantitatively and qualitatively as per the 2D case in both the linear and nonlinear collapse regimes. However, the scaling with resistivity of the 3D reconnection rate - which is a global quantity - is found to be less favourable when the magnetic null point is more rotationally symmetric, due to the action of increased magnetic back-pressure. Furthermore, we find that with increasing ambient plasma pressure the collapse can be throttled, as is the case for 2D nulls. We discuss this pressure-limiting in the context of fast reconnection in the solar atmosphere and suggest mechanisms by which it may be overcome. We also discuss the implications of the results in the context of null collapse as a trigger mechanism of Oscillatory Reconnection, a time-dependent reconnection mechanism, and also within the wider subject of wave-null point interactions. We conclude that, in general, increasingly rotationally-asymmetric nulls will be more favourable in terms of magnetic energy release via null collapse than their more symmetric counterparts., Comment: Accepted in ApJ, will be published gold open access, refer to main journal
- Published
- 2018
- Full Text
- View/download PDF
8. Three-dimensional oscillatory magnetic reconnection
- Author
-
Thurgood, J. O., Pontin, D. I., and McLaughlin, J. A.
- Subjects
Astrophysics - Solar and Stellar Astrophysics ,Physics - Fluid Dynamics ,Physics - Plasma Physics ,Physics - Space Physics - Abstract
Here we detail the dynamic evolution of localised reconnection regions about three-dimensional (3D) magnetic null points by using numerical simulation. We demonstrate for the first time that reconnection triggered by the localised collapse of a 3D null point due to an external MHD wave involves a self-generated oscillation, whereby the current sheet and outflow jets undergo a reconnection reversal process during which back-pressure formation at the jet heads acts to prise open the collapsed field before overshooting the equilibrium into an opposite-polarity configuration. The discovery that reconnection at fully 3D nulls can proceed naturally in a time-dependent and periodic fashion is suggestive that oscillatory reconnection mechanisms may play a role in explaining periodicity in astrophysical phenomena associated with magnetic reconnection, such as the observed quasi-periodicity of solar and stellar flare emission. Furthermore, we find a consequence of oscillatory reconnection is the generation of a plethora of freely-propagating MHD waves which escape the vicinity of the reconnection region, Comment: Accepted in ApJ, will be published gold open access, refer to main journal for animated content
- Published
- 2017
- Full Text
- View/download PDF
9. Using Open-Response Fraction Items to Explore the Relationship between Instructional Modalities and Students' Solution Strategies
- Author
-
Shumway, Jessica F., Moyer-Packenham, Patricia S., Baker, Joseph M., Westenskow, Arla, Anderson-Pence, Katie L., Tucker, Stephen I., Boyer-Thurgood, Jennifer, and Jordan, Kerry E.
- Abstract
The purpose of this study was to explore the relationship between instructional modality used for teaching fractions and third- and fourth-grade students' responses and strategies to open-response fraction items. The participants were 155 third-grade and 200 fourth-grade students from 17 public school classrooms. Students within each class were randomly assigned to two instructional treatment groups: a virtual manipulatives representations (VMR) instruction group and a physical manipulatives and textbook representations (PMTR) instruction group. A conversion mixed methods analysis was used to examine quantitative and qualitative data. The quantitative analysis showed achievement outcomes were the same for both groups. The qualitative analysis revealed shifts in learning that were otherwise hidden with solely quantitative achievement results. Specifically, the results indicated VMR group success in understanding fractions as relationships and PMTR group success in maintaining conceptualization of the whole. Overall, the results of this study corroborate previous research indicating the importance of both types of instructional modalities, showing that virtual manipulatives and physical manipulatives are effective instructional tools with positive effects on student learning. The study expands existing research by offering an opportunity to explore the nuances of students' fractions understanding and provide a window into students' shifts in fraction learning.
- Published
- 2016
10. Volatile Composition, Antimicrobial Activity, and In Vitro Innate Immunomodulatory Activity of Echinacea purpurea (L.) Moench Essential Oils
- Author
-
Noura S. Dosoky, Liliya N. Kirpotina, Igor A. Schepetkin, Andrei I. Khlebnikov, Brent L. Lisonbee, Jeffrey L. Black, Hillary Woolf, Trever L. Thurgood, Brittany L. Graf, Prabodh Satyal, and Mark T. Quinn
- Subjects
Echinacea purpurea ,purple coneflower ,essential oil ,antimicrobial activity ,(+)-δ-cadinene ,calcium flux ,Organic chemistry ,QD241-441 - Abstract
Echinacea purpurea (L.) Moench is a medicinal plant commonly used for the treatment of upper respiratory tract infections, the common cold, sore throat, migraine, colic, stomach cramps, and toothaches and the promotion of wound healing. Based on the known pharmacological properties of essential oils (EOs), we hypothesized that E. purpurea EOs may contribute to these medicinal properties. In this work, EOs from the flowers of E. purpurea were steam-distilled and analyzed by gas chromatography–mass spectrometry (GC–MS), GC with flame-ionization detection (GC–FID), and chiral GC–MS. The EOs were also evaluated for in vitro antimicrobial and innate immunomodulatory activity. About 87 compounds were identified in five samples of the steam-distilled E. purpurea EO. The major components of the E. purpurea EO were germacrene D (42.0 ± 4.61%), α-phellandrene (10.09 ± 1.59%), β-caryophyllene (5.75 ± 1.72%), γ-curcumene (5.03 ± 1.96%), α-pinene (4.44 ± 1.78%), δ-cadinene (3.31 ± 0.61%), and β-pinene (2.43 ± 0.98%). Eleven chiral compounds were identified in the E. purpurea EO, including α-pinene, sabinene, β-pinene, α-phellandrene, limonene, β-phellandrene, α-copaene, β-elemene, β-caryophyllene, germacrene D, and δ-cadinene. Analysis of E. purpurea EO antimicrobial activity showed that they inhibited the growth of several bacterial species, although the EO did not seem to be effective for Staphylococcus aureus. The E. purpurea EO and its major components induced intracellular calcium mobilization in human neutrophils. Additionally, pretreatment of human neutrophils with the E. purpurea EO or (+)-δ-cadinene suppressed agonist-induced neutrophil calcium mobilization and chemotaxis. Moreover, pharmacophore mapping studies predicted two potential MAPK targets for (+)-δ-cadinene. Our results are consistent with previous reports on the innate immunomodulatory activities of β-caryophyllene, α-phellandrene, and germacrene D. Thus, this study identified δ-cadinene as a novel neutrophil agonist and suggests that δ-cadinene may contribute to the reported immunomodulatory activity of E. purpurea.
- Published
- 2023
- Full Text
- View/download PDF
11. Investigating the effects of low intensity visible light on human keratinocytes using a customized LED exposure system
- Author
-
Sutterby, Emily, Chheang, Chanly, Thurgood, Peter, Khoshmanesh, Khashayar, Baratchi, Sara, and Pirogova, Elena
- Published
- 2022
- Full Text
- View/download PDF
12. Microfluidic models of the human circulatory system: versatile platforms for exploring mechanobiology and disease modeling
- Author
-
Nguyen, Ngan, Thurgood, Peter, Sekar, Nadia Chandra, Chen, Sheng, Pirogova, Elena, Peter, Karlheinz, Baratchi, Sara, and Khoshmanesh, Khashayar
- Published
- 2021
- Full Text
- View/download PDF
13. Particle-in-cell simulations of the relaxation of electron beams in inhomogeneous solar wind plasmas
- Author
-
Thurgood, J. O. and Tsiklauri, D.
- Subjects
Physics - Plasma Physics ,Astrophysics - Solar and Stellar Astrophysics ,Physics - Space Physics - Abstract
Previous theoretical considerations of electron beam relaxation in inhomogeneous plasmas have indicated that the effects of the irregular solar wind may account for the poor agreement of homogeneous modelling with the observations. Quasi-linear theory and Hamiltonian models based on Zakharov's equations have indicated that when a level of density fluctuations is above a given threshold, density irregularities act to de-resonate the beam-plasma interaction, restricting Langmuir wave growth on the expense of beam energy. This work presents the first fully kinetic particle-in-cell (PIC) simulations of beam relaxation under the influence of density irregularities. We aim to independently determine the influence of background inhomogeneity on the beam-plasma system, and to test theoretical predictions and alternative models using a fully kinetic treatment. We carry out 1D PIC simulations of a bump-on-tail unstable electron beam in the presence of increasing levels of background inhomogeneity using the fully electromagnetic, relativistic EPOCH PIC code. We find that in the case of homogeneous background plasma density, Langmuir wave packets are generated at the resonant condition and then quasi-liear relaxation leads to a dynamic increase of wavenumbers generated. No electron acceleration is seen - unlike in the inhomogeneous experiments, all of which produce high-energy electrons. For the inhomogeneous experiments we also observe the generation of backwards propagating Langmuir waves, which is shown directly to be due to the refraction of the packets off the density gradients. Our fully kinetic PIC simulations broadly confirm the findings of quasi-linear theory and the Hamiltonian model based on Zakharov's equations. Strong density fluctuations modify properties of excited Langmuir waves altering their dispersion properties., Comment: Animations can be found at https://jonathanthurgood.wordpress.com/myresearch/thurgood-and-tsiklauri-2016-movies/
- Published
- 2016
- Full Text
- View/download PDF
14. Understanding the Mineral Nutrient Value of Wheat Residue
- Author
-
Adams, Curtis B., Rogers, Christopher W., Marshall, Juliet M., Hatzenbuehler, Patrick, Walsh, Olga S., Thurgood, Garrett, Dari, Biswanath, Loomis, Grant, and Tarkalson, David
- Abstract
There is a substantial pool of mineral nutrients contained in wheat residue, concentrated in K, which has substantial economic value. Given this value, it is important for wheat producers to weigh the relative benefits of residue harvest, which gives immediate but marginal revenue gains, and residue retention, which has multifaceted benefits that include substantial savings on future nutrient costs. Persistent removal of nutrients from agronomic systems through residue harvest affects soil nutrient availability in the short‐ and long‐term, and the timing and magnitude of these changes will depend on the cropping system and soil. Earn 1 CEU in Nutrient Management by reading the article and taking the quiz at https://web.sciencesocieties.org/Learning‐Center/Courses.
- Published
- 2024
- Full Text
- View/download PDF
15. Self-consistent particle-in-cell simulations of fundamental and harmonic plasma radio emission mechanisms
- Author
-
Thurgood, J. O. and Tsiklauri, D.
- Subjects
Astrophysics - Solar and Stellar Astrophysics ,Physics - Plasma Physics - Abstract
Aims. The simulation of three-wave interaction based plasma emission, thought to be the underlying mechanism for Type III solar radio bursts, is a challenging task requiring fully-kinetic, multi-dimensional models. This paper aims to resolve a contradiction in past attempts, whereby some studies indicate that no such processes occur. Methods. We self-consistently simulate three-waved based plasma emission through all stages by using 2D, fully kinetic, electromagnetic particle-in-cell simulations of relaxing electron beams using the EPOCH2D code. Results. Here we present the results of two simulations; Run 1 (nb/n0 = 0.0057, vb/{\Delta}vb = vb/Ve = 16) and Run 2 (nb/n0 = 0.05, vb/{\Delta}vb = vb/Ve = 8), which we find to permit and prohibit plasma emission respectively. We show that the possibility of plasma emission is contingent upon the frequency of the initial electrostatic waves generated by the bump-in-tail instability, and that these waves may be prohibited from participating in the necessary three-wave interactions due to frequency conservation requirements. In resolving this apparent contradiction through a comprehensive analysis, in this paper we present the first self-consistent demonstration of fundamental and harmonic plasma emission from a single-beam system via fully kinetic numerical simulation. We caution against simulating astrophysical radio bursts using unrealistically dense beams (a common approach which reduces run time), as the resulting non-Langmiur characteristics of the initial wave modes significantly suppresses emission. Comparison of our results also indicates that, contrary to the suggestions of previous authors, an alternative plasma emission mechanism based on two counter-propagating beams is unnecessary in an astrophysical context.
- Published
- 2015
- Full Text
- View/download PDF
16. The Role of Cholesterol in Chronic Lymphocytic Leukemia Development and Pathogenesis
- Author
-
Alana M. White, Oliver G. Best, Anya K. Hotinski, Bryone J. Kuss, and Lauren A. Thurgood
- Subjects
chronic lymphocytic leukemia (CLL) ,statins ,cancer ,dyslipidemia ,LDL ,HDL ,Microbiology ,QR1-502 - Abstract
Cholesterol has many critical functions in cells. It is a key component of membranes and cell-signalling processes, and it functions as a chemical precursor in several biochemical pathways, such as Vitamin D and steroid synthesis. Cholesterol has also been implicated in the development and progression of various cancers, in which it is thought to promote cell proliferation, migration, and invasion. Chronic lymphocytic leukemia (CLL) is an example of a lipid-avid cancer that relies on lipid metabolism, rather than glycolysis, to fuel cell proliferation. However, data regarding the role of cholesterol in CLL are conflicting. Studies have shown that dyslipidaemia is more common among CLL patients than age-matched healthy controls, and that CLL patients who take cholesterol-lowering drugs, such as statins, appear to have improved survival rates. Therefore, defining the roles of cholesterol in CLL may highlight the importance of monitoring and managing hyperlipidaemia as part of the routine management of patients with CLL. In this review, we discuss the roles of cholesterol in the context of CLL by examining the literature concerning the trafficking, uptake, endogenous synthesis, and intracellular handling of this lipid. Data from clinical trials investigating various classes of cholesterol and lipid-lowering drugs in CLL are also discussed.
- Published
- 2023
- Full Text
- View/download PDF
17. In Memoriam: Robert Blust (1940-2022)
- Author
-
Graham Thurgood
- Subjects
obiturary ,Languages and literature of Eastern Asia, Africa, Oceania ,PL1-8844 - Abstract
This is an "in memoriam" for Robert Blust (1940-2022).
- Published
- 2022
18. First direct measurements of transverse waves in solar polar plumes using SDO/AIA
- Author
-
Thurgood, J. O., Morton, R. J, and McLaughlin, J. A.
- Subjects
Astrophysics - Solar and Stellar Astrophysics - Abstract
There is intense interest in determining the precise contribution of Alfv\'enic waves propagating along solar structures to the problems of coronal heating and solar wind acceleration. Since the launch of SDO/AIA, it has been possible to resolve transverse oscillations in off-limb solar polar plumes and recently McIntosh et al. (2011, Nature, 475, 477) concluded that such waves are energetic enough to play a role in heating the corona and accelerating the fast solar wind. However, this result is based on comparisons to Monte Carlo simulations and confirmation via direct measurements is still outstanding. Thus, this letter reports on the first direct measurements of transverse wave motions in solar polar plumes. Over a 4 hour period, we measure the transverse displacements, periods and velocity amplitudes of 596 distinct oscillations observed in the 171 \AA channel of SDO/AIA. We find a broad range of non-uniformly distributed parameter values which are well described by log-normal distributions with peaks at $234$ km, $121$ s and $8$ km s$^{-1}$, and mean and standard deviations of $407\pm297$ km, $173\pm118$ s and $14\pm10$ km s$^{-1}$. Within standard deviations, our direct measurements are broadly consistent with previous results. However, accounting for the whole of our observed non-uniform parameter distribution we calculate an energy flux of $9-24$ W m$^{-2}$, which is $4-10$ times below the energy requirement for solar wind acceleration. Hence, our results indicate that transverse MHD waves as resolved by SDO/AIA cannot be the dominant energy source for fast solar wind acceleration in the open-field corona., Comment: 16 pages, 4 figures, 1 table
- Published
- 2014
- Full Text
- View/download PDF
19. Evaluation of in vitro human skin models for studying effects of external stressors and stimuli and developing treatment modalities
- Author
-
Emily Sutterby, Peter Thurgood, Sara Baratchi, Khashayar Khoshmanesh, and Elena Pirogova
- Subjects
3D bioprinting ,drug development ,in vitro ,organoid ,skin model ,skin‐on‐chip ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
Abstract Skin is exposed to a variety of potential stressors and stimulators that may impact homeostasis, healing, tumor development, inflammation, and irritation. As such it is important to understand the impact that these stimuli have on skin health and function, and to develop therapeutic interventions. Animal experiments have been the gold standard for testing the safety and efficacy of therapeutics and observing disease pathology for centuries. However, complex ethics, costs, time consumption, and interspecies variation limit the transferability of results to humans and reduce their repeatability and reliability. Furthermore, traditional 2D cell studies are not representative of human tissue. Skin tissue is a dynamic environment, and when cells are isolated in unphysiologically stiff, static petri dishes their behavior, and phenotypic expression is altered. Increasingly complex in vitro models of human skin, including organoids, 3D bioprinting, and skin‐on‐a‐chip platforms, present the opportunity to gain insight into how stressors affect tissue at a cellular level in a controlled and repeatable environment. This insight can be leveraged to further understand pathological skin conditions and better formulate and validate drugs and therapeutics. Here, we will discuss the application of in vitro skin modeling to investigating the effects of mechanical, electromagnetic, and chemical stressors on skin.
- Published
- 2022
- Full Text
- View/download PDF
20. 3D Alfven wave behaviour around proper and improper magnetic null points
- Author
-
Thurgood, J. O. and McLaughlin, J. A.
- Subjects
Astrophysics - Solar and Stellar Astrophysics ,Physics - Fluid Dynamics ,Physics - Plasma Physics ,Physics - Space Physics - Abstract
Context: MHD waves and magnetic null points are both prevalent in many astrophysical plasmas, including the solar atmosphere. Interaction between waves and null points has been implicated as a possible mechanism for localised heating events. Aims: Here we investigate the transient behaviour of the Alfven wave about fully 3D proper and improper 3D magnetic null points. Previously, the behaviour of fast magnetoacoustic waves at null points in 3D, cold MHD was considered by Thurgood & McLaughlin (Astronomy & Astrophysics, 2012, 545, A9). Methods: We introduce an Alfven wave into the vicinity of both proper and improper null points by numerically solving the ideal, $\beta=0$ MHD equations using the LARE3D code. A magnetic fieldline and flux-based coordinate system permits the isolation of resulting wave-modes and the analysis of their interaction. Results: We find that the Alfven wave propagates throughout the region and accumulates near the fan-plane, causing current build up. For different values of null point eccentricity, the qualitative behaviour changes only by the imposition of anisotropic pulse dilation, due to the differing rates at which fieldlines diverge from the spine. For all eccentricities, we find that the fast and Alfven waves are linearly decoupled. During the driving phase, an independently propagating fast wave is nonlinearly generated due to the ponderomotive force. Subsequently, no further excitation of fast waves occurs. Conclusions: We find that the key aspects of the theory of Alfv\'en waves about 2D null points extends intuitively to the fully 3D case; i.e. the wave propagates along fieldlines and thus accumulates at predictable parts of the topology. We also highlight that unlike in the 2D case, in 3D Alfven-wave pulses are always toroidal and thus any aspects of 2D Alfven-wave-null models that are pulse-geometry specific must be reconsidered in 3D., Comment: Accepted for publication in Astronomy & Astrophysics
- Published
- 2013
- Full Text
- View/download PDF
21. Nonlinear Alfv\'en wave dynamics at a 2D magnetic null point: ponderomotive force
- Author
-
Thurgood, J. O. and McLaughlin, J. A.
- Subjects
Astrophysics - Solar and Stellar Astrophysics ,Physics - Fluid Dynamics ,Physics - Geophysics ,Physics - Plasma Physics - Abstract
Context : In the linear, {\beta}=0 MHD regime, the transient properties of MHD waves in the vicinity of 2D null points are well known. The waves are decoupled and accumulate at predictable parts of the magnetic topology: fast waves accumulate at the null point; whereas Alfv\'en waves cannot cross the separatricies. However, in nonlinear MHD mode conversion can occur at regions of inhomogeneous Alfv\'en speed, suggesting that the decoupled nature of waves may not extend to the nonlinear regime. Aims: We investigate the behaviour of low-amplitude Alfv\'en waves about a 2D magnetic null point in nonlinear, {\beta}= 0 MHD. Methods: We numerically simulate the introduction of low-amplitude Alfv\'en waves into the vicinity of a magnetic null point using the nonlinear LARE2D code. Results: Unlike in the linear regime, we find that the Alfv\'en wave sustains cospatial daughter disturbances, manifest in the transverse and longitudinal fluid velocity, owing to the action of nonlinear magnetic pressure gradients (viz. the ponderomotive force). These disturbances are dependent on the Alfv\'en wave and do not interact with the medium to excite magnetoacoustic waves, although the transverse daughter becomes focused at the null point. Additionally, an independently propagating fast magnetoacoustic wave is generated during the early stages, which transports some of the initial Alfv\'en wave energy towards the null point. Subsequently, despite undergoing dispersion and phase-mixing due to gradients in the Alfv\'en-speed profile (Grad c_A \neq 0) there is no further nonlinear generation of fast waves. Conclusions: We find that Alfv\'en waves at 2D cold null points behave largely as in the linear regime, however they sustain transverse and longitudinal disturbances - effects absent in the linear regime - due to nonlinear magnetic pressure gradients., Comment: 7 pages. 4 figures, Pre-print: Accepted for publication in Astronomy & Astrophysics
- Published
- 2013
- Full Text
- View/download PDF
22. On ponderomotive effects induced by Alfven waves in inhomogeneous 2.5D MHD plasmas
- Author
-
Thurgood, J. O. and McLaughlin, J. A.
- Subjects
Astrophysics - Solar and Stellar Astrophysics - Abstract
Where spatial gradients in the amplitude of an Alfven wave are non-zero, a nonlinear magnetic-pressure gradient acts upon the medium (the ponderomotive force). We investigate the nature of such a force in inhomogeneous 2.5D MHD plasmas by analysing source terms in the nonlinear wave equations for the general case of inhomogeneous B and {\rho}, and consider supporting nonlinear numerical simulations. Our equations indicate there are two distinct classes of ponderomotive effect induced by Alfven waves in general 2.5D MHD, each with both a longitudinal and transverse manifestation; i) Geometric Effects: Gradients in the pulse geometry relative to the background magnetic field cause the wave to sustain cospatial disturbances, the longitudinal and transverse daughter disturbances - where we report on the transverse disturbance for the first time. ii) Grad (c_A) Effects: Where a pulse propagates through an inhomogeneous region (where the non-zero gradients in the Alfven-speed profile are non-zero), the nonlinear magnetic-pressure gradient acts to accelerate the plasma. Transverse gradients (phase mixing regions) excite independently propagating fast magnetoacoustic waves (generalising the result of Nakariakov et al. 1997, Solar Physics, 175, 93) and longitudinal gradients perturb along the field (thus creating static disturbances in {\beta} = 0, and slow waves in {\beta} \= 0). We additionally demonstrate that mode conversion due the nonlinear Lorentz force is a one-way process, and does not act as a mechanism to nonlinearly generate Alfven waves due to propagating magnetoacoustic waves. We conclude that these ponderomotive effects are induced by an Alfven wave propagating in any MHD medium, and have the potential to have significant consequences on the dynamics of energy transport and aspects of dissipation provided the system is sufficiently nonlinear and inhomogeneous., Comment: Pre-print, Accepted for publication in Solar Physics Feb 2013
- Published
- 2013
- Full Text
- View/download PDF
23. Studying the Mechanobiology of Aortic Endothelial Cells Under Cyclic Stretch Using a Modular 3D Printed System
- Author
-
Sergio Aguilera Suarez, Nadia Chandra Sekar, Ngan Nguyen, Austin Lai, Peter Thurgood, Ying Zhou, Scott Needham, Elena Pirogova, Khashayar Khoshmanesh, and Sara Baratchi
- Subjects
mechanobiology ,endothelial ,cyclic stretch ,3D printing ,mechanotransduction ,biomechanics ,Biotechnology ,TP248.13-248.65 - Abstract
Here, we describe a motorized cam-driven system for the cyclic stretch of aortic endothelial cells. Our modular design allows for generating customized spatiotemporal stretch profiles by varying the profile and size of 3D printed cam and follower elements. The system is controllable, compact, inexpensive, and amenable for parallelization and long-term experiments. Experiments using human aortic endothelial cells show significant changes in the cytoskeletal structure and morphology of cells following exposure to 5 and 10% cyclic stretch over 9 and 16 h. The system provides upportunities for exploring the complex molecular and cellular processes governing the response of mechanosensitive cells under cyclic stretch.
- Published
- 2021
- Full Text
- View/download PDF
24. On the Periodicity of Oscillatory Reconnection
- Author
-
McLaughlin, J. A., Thurgood, J. O., and MacTaggart, D.
- Subjects
Astrophysics - Solar and Stellar Astrophysics - Abstract
Oscillatory reconnection is a time-dependent magnetic reconnection mechanism that naturally produces periodic outputs from aperiodic drivers. This paper aims to quantify and measure the periodic nature of oscillatory reconnection for the first time. We solve the compressible, resistive, nonlinear MHD equations using 2.5D numerical simulations. We identify two distinct periodic regimes: the impulsive and stationary phases. In the impulsive phase, we find the greater the amplitude of the initial velocity driver, the longer the resultant current sheet and the earlier its formation. In the stationary phase, we find that the oscillations are exponentially decaying and for driving amplitudes 6.3 - 126.2 km/s, we measure stationary-phase periods in the range 56.3 - 78.9 s, i.e. these are high frequency (0.01 - 0.02 Hz) oscillations. In both phases, we find that the greater the amplitude of the initial velocity driver, the shorter the resultant period, but note that different physical processes and periods are associated with both phases. We conclude that the oscillatory reconnection mechanism behaves akin to a damped harmonic oscillator., Comment: 8 pages, 4 figures
- Published
- 2012
- Full Text
- View/download PDF
25. Linear and nonlinear MHD mode coupling of the fast magnetoacoustic wave about a 3D magnetic null point
- Author
-
Thurgood, J. O. and McLaughlin, J. A.
- Subjects
Astrophysics - Solar and Stellar Astrophysics ,Physics - Fluid Dynamics ,Physics - Plasma Physics ,76W05, 85A30 - Abstract
Context: Coronal magnetic null points have been implicated as possible locations for localised heating events in 2D models. We investigate this possibility about fully 3D null points. Aims: We investigate the nature of the fast magnetoacoustic wave about a fully 3D magnetic null point, with a specific interest in its propagation, and we look for evidence of MHD mode coupling and/or conversion to the Alfv\'en mode. Methods: A special fieldline and flux-based coordinate system was constructed to permit the introduction of a pure fast magnetoacoustic wave in the vicinity of proper and improper 3D null points. We considered the ideal, {\beta} = 0, MHD equations, which are solved using the LARE3D numerical code. The constituent modes of the resulting wave were isolated and identified using the special coordinate system. Numerical results were supported by analytical work derived from perturbation theory and a linear implementation of the WKB method. Results: An initially pure fast wave is found to be permanently decoupled from the Alfv\'en mode both linearly and nonlinearly for both proper and improper 3D null points. The pure fast mode also generates and sustains a nonlinear disturbance aligned along the equilibrium magnetic field. The resulting pure fast magnetoacoustic pulse has transient behaviour, which is found to be governed by the (equilibrium) Alfv\'en-speed profile, and a refraction effect focuses all the wave energy towards the null point. Conclusions: Thus, the main results from previous 2D work do indeed carry over to the fully 3D magnetic null points and, Comment: 11 pages, 6 figures
- Published
- 2012
- Full Text
- View/download PDF
26. Barley residue biomass, nutrient content, and relationships with grain yield
- Author
-
Rogers, Christopher W., Adams, Curtis B., Marshall, Juliet M., Hatzenbuehler, Patrick, Thurgood, Garrett, Dari, Biswanath, Loomis, Grant, and Tarkalson, David D.
- Abstract
Determination of barley (Hordeum vulgareL.) nutrient uptake in residue biomass is important for agronomic, economic, and environmental decisions. Improved understanding of grain yield, residue biomass, nutrient uptake, and their relationships are needed. Research determined these factors in 2018 and 2019 from trials of four barley classes (spring animal feed, human food, and malt, as well as winter malt), using three common cultivars of each, at five locations in southern Idaho. Production environment created the largest difference in residue biomass and nutrient uptake. Barley harvest index ranged from 0.46 to 0.52 Mg Mg−1across feed, food, and malt classes. Compared to previous estimates, nutrient concentrations from the combined dataset were greater than for N, less than for P, and greater than for K. Correlation of grain yields to nutrient uptake (excluding Cu and Fe) had r2ranging from 0.68 to 0.89. At current prices, economic analysis indicated that fertilizer replacement costs for total residue biomass nutrients were greater than previous estimates and could greatly exceed current sale value. These relationships and value estimates can be used to improve prediction of barley residue biomass production and nutrient uptake to guide best management practices. The decision of how to utilize these metrics (on‐farm, regional, etc.) should be considered based on known variation in measured nutrient and residue data and considered in relation to the proposed task. Limited research has focused on nutrient uptake and economic value of barley residue biomass (straw + chaff).Production environment created the largest differences in residue biomass and nutrient uptake across barley classes.Average harvest indices (HI) were 0.51, 0.47, 0.52, and 0.46 Mg Mg−1for feed, food, spring malt, and winter malt barley.N uptake combined across classes was greater than previous estimates, P was less, and K was greater.Fertilizer replacement cost of nutrients removed in residue may far exceed the sale value.
- Published
- 2024
- Full Text
- View/download PDF
27. Improving Estimates for Barley Residue Biomass, Nutrients, and Economic Value
- Author
-
Rogers, Christopher W., Adams, Curtis B., Tarkalson, David D., Marshall, Juliet M., Hatzenbuehler, Patrick, Thurgood, Garrett, Dari, Biswanath, and Loomis, Grant
- Abstract
Global market demands for barley are less than cereals such as corn, rice, and wheat, but barley plays a particularly important role in cropping systems in areas of high elevation and/or latitude with short growing seasons. Estimation of residue nutrient uptake requires both a knowledge of the biomass produced, as well as the concentration of individual nutrients. These factors were evaluated in 2018 and 2019 from trials of four barley classes at five locations in southern Idaho. Earn 0.5 CEUs in Crop Management by reading the article and taking the quiz at https://web.sciencesocieties.org/Learning‐Center/Courses.
- Published
- 2024
- Full Text
- View/download PDF
28. Development Plan for Relocation to the Nichols Avenue School
- Author
-
Thurgood Marshall Academy Public Charter School, JFW Project Management, 21st Century School Fund, Washington, DC
- Abstract
In this report, Thurgood Marshall Academy Public Charter High School (TMA) submits its Development Plan for the renovation and improvements to the Nichols Avenue School building and site, which will serve as the future home for their school. Working with an experienced planning, design and construction team, they have prepared a Development Plan that will revitalize this important corner, provide a state of the art educational environment for TMA and Savoy students, and provide recreational amenities to the youth and adults of the neighborhood.
- Published
- 2004
29. NCES Handbook of Survey Methods: Technical Report.
- Author
-
National Center for Education Statistics (ED), Washington, DC., Synectics for Management Decisions, Inc., Arlington, VA., Thurgood, Lori, Walter, Elizabeth, and Carter, George
- Abstract
This handbook presents current explanations of how each survey program of the National Center for Education Statistics (NCES) obtains and prepared the data it publishes. The handbook aims to provide users of NCES data with the most current information necessary to evaluate the suitability of the statistics for their needs, with a focus on the methodologies for survey design, data collection, and data processing. The handbook contains 18 chapters. Chapters 1 to 26 each focus on one of the 26 major NCES survey programs. Information in these chapters is presented in a uniform format of: (1) overview; (2) uses of data; (3) key concepts; (4) survey design; (5) data quality and comparability; (6) contact information; and (7) methodology and evaluation reports. These chapters are organized into these subject matter categories: (1) early childhood education survey; (2) elementary and secondary education surveys; (3) library surveys; (4) postsecondary and adult education surveys; (5) educational assessment surveys; and (6) household education surveys. The final chapters, 27 and 28, cover multiple surveys and survey systems, including the Fast Response Survey System. Five appendixes contain a glossary, information on how to obtain NCES publications, a list of tools for each survey, a list of Web sites for each survey, and an index. (SLD)
- Published
- 2003
30. 1997 Customer Satisfaction Survey Report: How Do We Measure Up? Technical Report. Survey Report, 1997.
- Author
-
National Center for Education Statistics (ED), Washington, DC., Synectics for Management Decisions, Inc., Arlington, VA., Thurgood, Lori, Fink, Steven, Bureika, Rita, Scott, Julie, and Salvucci, Sameena
- Abstract
The 1997 National Center for Education Statistics (NCES) Customer Satisfaction survey was conducted to find out whether the NCES as an agency was responding to the needs of customers and to identify areas for improvement. Federal, state, and local education officials and academic researchers were asked about their satisfaction with NCES products and services and about other organizations from which they receive education data. There were 2,948 eligible individuals in the sample, and responses were received from 2,465 (84%). Local policymakers are a key customer group, yet only half were current users of NCES products and services. A majority of users were frequent users, and a very high percentage of respondents reported being satisfied or very satisfied with NCES publications and reports, and these publications and reports received high marks on most aspects. Users were satisfied with all aspects of NCES publications except timeliness. NCES data files were not as widely used as reports and publications, but were ranked as highly. Users were most dissatisfied with the timeliness and use of data files. Most customers did not know how to contact the NCES and were not fully aware of the broad range of services. NCES users most often used their State Department of Education as another source of education data. Local policymakers were apt to prefer their benchmarks organizations and rate them more highly than the NCES, but federal and state policymakers did not rate benchmark organizations above the NCES. Overall, NCES did well in comparison with the best of the rest, but areas for improvement were noted. Four appendixes contain supplemental information, including the survey. (Contains 30 figures and 28 tables.) (SLD)
- Published
- 1999
31. Doctorate Recipients from United States Universities. Summary Report, 1994.
- Author
-
National Academy of Sciences - National Research Council, Washington, DC. Office of Scientific and Engineering Personnel., Simmons, Robert O., and Thurgood, Delores H.
- Abstract
This 28th annual report summarizes results of the 1993-94 Survey of Earned Doctorates, which collected data from graduates as they completed requirements for their doctoral degrees. The survey found 41,011 doctorates (88 percent Ph.D degrees and most of the rest Ed.D.s) were awarded. Demographic highlights included: a record number (15,806) of Ph.D.s earned by women; U.S. citizens earned over two-thirds of the doctorates; most non-citizens earning the degree had temporary visas; and citizens of the People's Republic of China accounted for 21 percent of all non-U.S. Ph.D. recipients. Data are presented in 20 tables covering number of doctorates awarded, gender of recipients, citizenship status, leading countries of origins of non-U.S. citizens, leading U.S. baccalaureate and doctoral institutions of minority doctorate recipients, major doctoral field, major field of U.S. citizen doctoral recipients by race/ethnicity; median years to doctorate, primary sources of support, levels of debt, post-graduation commitments, postdoctoral location of non-U.S. citizen doctoral recipients, and employment sector of doctorate recipients. The report also analyzes trends in doctorate production including general demographic characteristics of doctorate recipients in terms of gender, citizenship, and racial/ethnic minority status; field of doctorate; time-to-degree; financial support; and post-graduation plans. Appendixes provide supplementary tables on 1994 doctorate recipients, trend data on 1984-94 doctoral cohorts, technical notes, and a copy of the survey questionnaire. (JB)
- Published
- 1995
32. Doctorate Recipients from United States Universities. Summary Report 1993.
- Author
-
National Academy of Sciences - National Research Council, Washington, DC. Office of Scientific and Engineering Personnel., Thurgood, Delores H., and Clarke, Julie E.
- Abstract
This 27th annual report summarizes results of the 1992-93 Survey of Earned Doctorates, which collected data from graduates as they completed requirements for their doctoral degrees. The survey included 39,754 research and applied research doctorates in physical sciences, engineering, life sciences, social sciences, humanities, education, and other professional (such as business) fields. Data are presented in 19 tables, covering number of doctorates awarded, gender of doctorate recipients, citizenship status, leading countries of origin of non-U.S. citizens, leading U.S. baccalaureate and doctoral institutions of minority doctorate recipients, major doctorate field, median years to doctorate, primary sources of support, levels of debt, postgraduation commitments, postdoctoral location of non-U.S. citizen doctorate recipients, and employment sector of doctorate recipients. The report also analyzes trends in doctorate production from 1963 to 1993; general demographic characteristics of doctorate recipients in terms of gender, citizenship, and racial/ethnic minority status; the field of doctorate; time-to-degree; financial support; and postgraduation plans. Appendices provide supplementary tables on 1993 doctorate recipients, trend data on 1983-1993 doctoral cohorts, technical notes, and a copy of the survey questionnaire. (JDD)
- Published
- 1995
33. Doctorate Recipients from United States Universities. Summary Report 1991.
- Author
-
National Academy of Sciences - National Research Council, Washington, DC. Office of Scientific and Engineering Personnel., Ries, Paula, and Thurgood, Delores
- Abstract
This report presents a summary of the 1990-91 results of the national Survey of Earned Doctorates conducted each year since 1958. Organized into three sections, the report first presents an analysis of trends in the numbers of doctorate recipients including data with regard to doctoral fields, gender, citizenship status, race and ethnicity, time-to-degree, and post-graduation plans. A second section on financial supports examines primary sources of support (personal, university, federal or other) and indebtedness (by gender, citizenship status and race). The final section discusses the increasing participation of women in U.S. doctoral education, with attention to trends in numbers and proportions of doctorates, fields of doctorate, degree-granting institutions, sources of support (personal, university, federal, debt), postdoctoral plans, and employment sector. Four appendixes present basic tables, trend tables for 1981 to 1991, technical notes, and the survey instrument. (JB)
- Published
- 1993
34. Doctorate Recipients from United States Universities. Summary Report 1990.
- Author
-
National Academy of Sciences - National Research Council, Washington, DC. Office of Scientific and Engineering Personnel., Thurgood, Delores, and Weinman, Joanne M.
- Abstract
This report presents a summary of the 1989-90 results of the Survey of Earned Doctorates conducted each year since 1958. Organized into three sections, the report first presents an analysis of trends in the numbers of doctorate recipients including data with regard to fields, gender, citizenship status, time-to-degree, and post-graduation plans (status, postdoctoral location and plans, employment sector). A second section on financial support examines primary sources of support and indebtedness. The final section discusses trends among United States citizen minority doctorates including trends in numbers and proportions of doctorates, doctoral fields, gender, parents' education, degree-granting institutions, primary sources of support, indebtedness, time-to-degree, post-graduation plans, and a summary and tables. Data on five groups are reported: Asians, blacks, Hispanics, Native Americans, and whites. Minorities earned 9.4 percent of doctorates in 1990 and in 1975 they earned 6.3 percent. The most significant gains were among Asians, Native Americans, and Hispanics. Blacks experienced a 17 percent decrease in doctoral degrees received between 1975 and 1990. Four appendixes present basic tables, trend tables for 1980 through 1990, technical notes and the survey instrument. (JB)
- Published
- 1991
35. Doctorate Recipients from United States Universities. Summary Report 1989.
- Author
-
National Academy of Sciences - National Research Council, Washington, DC. Office of Scientific and Engineering Personnel., Thurgood, Delores H., and Weinman, Joanne M.
- Abstract
This report presents a summary of the results of the 1988-89 Survey of Earned Doctorates conducted each year since 1958 and consisting of surveys filled out by graduates earning doctoral degrees. Organized into three sections, the report first presents a trend analysis of the numbers of doctorate recipients including data with regard to field, gender, citizenship status, race and ethnicity, time-to-degree, and post-graduation plans. A second section on financial supports examines primary sources of support (personal, university, federal or other) and indebtedness (by gender, citizenship status and race). The final section discusses the increasing participation of foreign citizens in U.S. doctoral education, with attention to numbers of such students, country of origin, field of doctorate, sources of support, postdoctoral location and plans, employment sector in the United States labor force, and work activity. Four appendixes present basic tables, trend tables, technical notes on data collection and a copy of the survey. The document contains 22 tables and 11 figures. (JB)
- Published
- 1990
36. Using Virtual Manipulatives on iPads to Promote Young Children's Mathematics Learning
- Author
-
Moyer-Packenham, Patricia S., Shumway, Jessica F., Bullock, Emma P., Anderson-Pence, Katie L., Tucker, Stephen I., Westenskow, Arla, Boyer-Thurgood, Jennifer, Gulkilik, Hilal, Watts, Christina, and Jordan, Kerry
- Abstract
The purpose of this project was to examine young children's mathematics learning using virtual manipulative touch-screen apps. We interviewed over 100 young children as they interacted with mathematics apps on iPads and examined how these interactions supported mathematics learning and contributed to changes in efficiency. Children interacted with six different mathematics apps under the direction of an interviewer. Results of the study indicated that children's learning and efficiency improved between the pre- and post-assessments during a short interview time period. Close alignment of the learning apps with the pre- and post-assessment apps contributed to these positive changes. To conduct this study, researchers created a variety of research tools that were not available because iPad research is still in its infancy. [For a related article, "Young Children's Learning Performance and Efficiency When Using Virtual Manipulative Mathematics iPad Apps," see EJ1048462.]
- Published
- 2016
37. The Role of Affordances in Children's Learning Performance and Efficiency When Using Virtual Manipulative Mathematics Touch-Screen Apps
- Author
-
Moyer-Packenham, Patricia S., Bullock, Emma K., Shumway, Jessica F., Tucker, Stephen I., Watts, Christina M., Westenskow, Arla, Anderson-Pence, Katie L., Maahs-Fladung, Cathy, Boyer-Thurgood, Jennifer, Gulkilik, Hilal, and Jordan, Kerry
- Abstract
This paper focuses on understanding the role that affordances played in children's learning performance and efficiency during clinical interviews of their interactions with mathematics apps on touch-screen devices. One hundred children, ages 3 to 8, each used six different virtual manipulative mathematics apps during 30-40-min interviews. The study used a convergent mixed methods design, in which quantitative and qualitative data were collected concurrently to answer the research questions (Creswell and Plano Clark 2011). Videos were used to capture each child's interactions with the virtual manipulative mathematics apps, document learning performance and efficiency, and record children's interactions with the affordances within the apps. Quantitized video data answered the research question on differences in children's learning performance and efficiency between pre- and post-assessments. A Wilcoxon matched pairs signed-rank test was used to explore these data. Qualitative video data was used to identify affordance access by children when using each app, identifying 95 potential helping and hindering affordances among the 18 apps. The results showed that there were changes in children's learning performance and efficiency when children accessed a helping or a hindering affordance. Helping affordances were more likely to be accessed by children who progressed between the pre- and post-assessments, and the same affordances had helping and hindering effects for different children. These results have important implications for the design of virtual manipulative mathematics learning apps.
- Published
- 2016
- Full Text
- View/download PDF
38. Dynamics of high viscosity contrast confluent microfluidic flows
- Author
-
Michael E. Kurdzinski, Berrak Gol, Aaron Co Hee, Peter Thurgood, Jiu Yang Zhu, Phred Petersen, Arnan Mitchell, and Khashayar Khoshmanesh
- Subjects
Medicine ,Science - Abstract
Abstract The laminar nature of microfluidic flows is most elegantly demonstrated via the confluence of two fluids forming two stable parallel flows within a single channel meeting at a highly stable interface. However, maintenance of laminar conditions can become complicated when there is a large viscosity contrast between the neighbouring flows leading to unique instability patterns along their interface. Here, we study the dynamics of high viscosity contrast confluent flows – specifically a core flow made of highly viscous glycerol confined by sheath flows made of water within a microfluidic flow focusing system. Our experiments indicate the formation of tapered core structures along the middle of the channel. Increasing the sheath flow rate shortens the tapered core, and importantly induces local instability patterns along the interface of core-sheath flows. The dynamics of such tapered core structures is governed by the intensity of instability patterns and the length of the core, according to which the core structure can experience stable, disturbed, broken or oscillated regimes. We have studied the dynamics of tapered core structures under these regimes. In particular, we have analysed the amplitude and frequency of core displacements during the broken core and oscillating core regimes, which have not been investigated before.
- Published
- 2017
- Full Text
- View/download PDF
39. Dynamic Vortex Generation, Pulsed Injection, and Rapid Mixing of Blood Samples in Microfluidics Using the Tube Oscillation Mechanism
- Author
-
Thurgood, P, Needham, S, Pirogova, E, Peter, K, Baratchi, S, Khoshmanesh, K, Thurgood, P, Needham, S, Pirogova, E, Peter, K, Baratchi, S, and Khoshmanesh, K
- Abstract
Here, we describe the generation of dynamic vortices in micro-scale cavities at low flow rates. The system utilizes a computer-controlled audio speaker to axially oscillate the inlet tube of the microfluidic system at desired frequencies and amplitudes. The oscillation of the tube induces transiently high flow rates in the system, which facilitates the generation of dynamic vortices inside the cavity. The size of the vortices can be modulated by varying the tube oscillation frequency or amplitude. The vortices can be generated in single or serial cavities and in a wide range of cavity sizes. We demonstrate the suitability of the tube oscillation mechanism for the pulsed injection of water-based solutions or whole blood into the cavity. The injection rate can be controlled by the oscillation characteristics of the tube, enabling the injection of liquids at ultralow flow rates. The dynamic vortices facilitate the rapid mixing of the injected liquid with the main flow. The controllability and versatility of this technology allow for the development of programmable inertial microfluidic systems for performing multistep biological assays.
- Published
- 2023
40. Tube Oscillation Drives Transitory Vortices Across Microfluidic Barriers
- Author
-
Thurgood, P, Hawke, A, Low, LS, Borg, A, Peter, K, Baratchi, S, Khoshmanesh, K, Thurgood, P, Hawke, A, Low, LS, Borg, A, Peter, K, Baratchi, S, and Khoshmanesh, K
- Abstract
Here, the generation of dynamic vortices across microscale barriers using the tube oscillation mechanism is demonstrated. Using a combination of high-speed imaging and computational flow dynamics, the cyclic formation, expansion, and collapse of vortices are studied. The dynamics of vortices across circular , triangular, and blade-shape barriers are investigated at different tube oscillation frequencies. The formation of an array of synchronous vortices across parallel blade-shaped barriers is demonstrated. The transient flows caused by these dynamic vortex arrays are harnessed for the rapid and efficient mixing of blood samples . A circular barrier scribed with a narrow orifice on its shoulder is used to facilitate the injection of liquid into the microfluidic channel, and its rapid mixing with the main flow through the dynamic vortices generated across the barrier. This approach facilitates the generation of vortices with desirable configurations, sizes, and dynamics in a highly controllable, programmable, and predictable manner while operating at low static flow rates.
- Published
- 2023
41. A 3D printed flow sensor for microfluidic applications
- Author
-
Hawke, A, Concilia, G, Thurgood, P, Ahnood, A, Baratchi, S, Khoshmanesh, K, Hawke, A, Concilia, G, Thurgood, P, Ahnood, A, Baratchi, S, and Khoshmanesh, K
- Published
- 2023
42. Endothelial Response to the Combined Biomechanics of Vessel Stiffness and Shear Stress Is Regulated via Piezo1
- Author
-
Lai, A, Zhou, Y, Thurgood, P, Chheang, C, Sekar, NC, Nguyen, N, Peter, K, Khoshmanesh, K, Baratchi, S, Lai, A, Zhou, Y, Thurgood, P, Chheang, C, Sekar, NC, Nguyen, N, Peter, K, Khoshmanesh, K, and Baratchi, S
- Abstract
How endothelial cells sense and respond to dynamic changes in their biophysical surroundings as we age is not fully understood. Vascular stiffness is clearly a contributing factor not only in several cardiovascular diseases but also in physiological processes such as aging and vascular dementia. To address this gap, we utilized a microfluidic model to explore how substrate stiffness in the presence of shear stress affects endothelial morphology, senescence, proliferation, and inflammation. We also studied the role of mechanosensitive ion channel Piezo1 in endothelial responses under the combined effect of shear stress and substrate stiffness. To do so, we cultured endothelial cells inside microfluidic channels covered with fibronectin-coated elastomer with elastic moduli of 40 and 200 kPa, respectively, mimicking the stiffness of the vessel walls in young and aged arteries. The endothelial cells were exposed to atheroprotective and atherogenic shear stress levels of 10 and 2 dyn/cm2, respectively. Our findings show that substrate stiffness affects senescence under atheroprotective flow conditions and cytoskeleton remodeling, senescence, and inflammation under atherogenic flow conditions. Additionally, we found that the expression of Piezo1 plays a crucial role in endothelial adaptation to flow and regulation of inflammation under both atheroprotective and atherogenic shear stress levels. However, Piezo1 contribution to endothelial senescence was limited to the soft substrate and atheroprotective shear stress level. Overall, our study characterizes the response of endothelial cells to the combined effect of shear stress and substrate stiffness and reveals a previously unidentified role of Piezo1 in endothelial response to vessel stiffening, which potentially can be therapeutically targeted to alleviate endothelial dysfunction in aging adults.
- Published
- 2023
43. A Microfluidic System for Studying the Effects of Disturbed Flow on Endothelial Cells
- Author
-
Francisco Tovar-Lopez, Peter Thurgood, Christopher Gilliam, Ngan Nguyen, Elena Pirogova, Khashayar Khoshmanesh, and Sara Baratchi
- Subjects
microfluidics ,endothelial cells (EC) ,disturbed flow ,shear stress ,actin stress fiber ,Biotechnology ,TP248.13-248.65 - Abstract
Arterial endothelium experience physical stress associated with blood flow and play a central role in maintaining vascular integrity and homeostasis in response to hemodynamic forces. Blood flow within vessels is generally laminar and streamlined. However, abrupt changes in the vessel geometry due to branching, sharp turns or stenosis can disturb the laminar blood flow, causing secondary flows in the form of vortices. Such disturbed flow patterns activate pro-inflammatory phenotypes in endothelial cells, damaging the endothelial layer and can lead to atherosclerosis and thrombosis. Here, we report a microfluidic system with integrated ridge-shaped obstacles for generating controllable disturbed flow patterns. This system is used to study the effect of disturbed flow on the cytoskeleton remodeling and nuclear shape and size of cultured human aortic endothelial cells. Our results demonstrate that the generated disturbed flow changes the orientation angle of actin stress fibers and reduces the nuclear size while increases the nuclear circularity.
- Published
- 2019
- Full Text
- View/download PDF
44. The TRPV4 Agonist GSK1016790A Regulates the Membrane Expression of TRPV4 Channels
- Author
-
Sara Baratchi, Peter Keov, William G. Darby, Austin Lai, Khashayar Khoshmanesh, Peter Thurgood, Parisa Vahidi, Karin Ejendal, and Peter McIntyre
- Subjects
TRPV4 ,membrane trafficking ,endothelial cells ,GSK1016790A ,calcium ,Therapeutics. Pharmacology ,RM1-950 - Abstract
TRPV4 is a non-selective cation channel that tunes the function of different tissues including the vascular endothelium, lung, chondrocytes, and neurons. GSK1016790A is the selective and potent agonist of TRPV4 and a pharmacological tool that is used to study the TRPV4 physiological function in vitro and in vivo. It remains unknown how the sensitivity of TRPV4 to this agonist is regulated. The spatial and temporal dynamics of receptors are the major determinants of cellular responses to stimuli. Membrane translocation has been shown to control the response of several members of the transient receptor potential (TRP) family of ion channels to different stimuli. Here, we show that TRPV4 stimulation with GSK1016790A caused an increase in [Ca2+]i that is stable for a few minutes. Single molecule analysis of TRPV4 channels showed that the density of TRPV4 at the plasma membrane is controlled through two modes of membrane trafficking, complete, and partial vesicular fusion. Further, we show that the density of TRPV4 at the plasma membrane decreased within 20 min, as they translocate to the recycling endosomes and that the surface density is dependent on the release of calcium from the intracellular stores and is controlled via a PI3K, PKC, and RhoA signaling pathway.
- Published
- 2019
- Full Text
- View/download PDF
45. A Window into Mathematical Support: How Parents' Perceptions Change Following Observations of Mathematics Tutoring
- Author
-
Westenskow, Arla, Boyer-Thurgood, Jennifer, and Moyer-Packenham, Patricia S.
- Abstract
This research study examined the perceptions of 24 parents of rising 5th-grade students with mathematics learning difficulties as part of a 10-week summer mathematics tutoring experience. During the summer tutoring program, parents observed their children participating in mathematics learning experiences during one-to-one tutoring sessions. At the conclusion of the summer tutoring services, parents reflected on observations of their own children in surveys and interviews. Results indicated that parents' observations of the tutoring impacted their perceptions and changed the support they provided to their children during and after the tutoring program. Parents reported changes in attitudes and beliefs about mathematics, gaining insights into their child's mathematical understanding, and changes in their methods and practices as mathematics supporters outside of the tutoring sessions.
- Published
- 2015
- Full Text
- View/download PDF
46. Asynchronous generation of oil droplets using a microfluidic flow focusing system
- Author
-
Thurgood, Peter, Baratchi, Sara, Arash, Aram, Pirogova, Elena, Jex, Aaron R., and Khoshmanesh, Khashayar
- Published
- 2019
- Full Text
- View/download PDF
47. Endothelial Response to the Combined Biomechanics of Vessel Stiffness and Shear Stress Is Regulated via Piezo1.
- Author
-
Lai, Austin, Zhou, Ying, Thurgood, Peter, Chheang, Chanly, Chandra Sekar, Nadia, Nguyen, Ngan, Peter, Karlheinz, Khoshmanesh, Khashayar, and Baratchi, Sara
- Published
- 2023
- Full Text
- View/download PDF
48. Bioengineered Vascular Model of Foam Cell Formation
- Author
-
Zhou, Ying, Sekar, Nadia Chandra, Thurgood, Peter, Needham, Scott, Peter, Karlheinz, Khoshmanesh, Khashayar, and Baratchi, Sara
- Abstract
Foam cell formation is a complex blood vessel pathology, which is characterized by a series of events, including endothelium dysfunction, inflammation, and accumulation of immune cells underneath the blood vessel walls. Novel bioengineered models capable of recapitulating these events are required to better understand the complex pathological processes underlying the development of foam cell formation and, consequently, advanced bioengineered platforms for screening drugs. Here, we generated a microfluidic blood vessel model, incorporating a three-dimensional (3D) extracellular matrix coated with an endothelial layer. This system enables us to perform experiments under a dynamic microenvironment that recapitulates the complexities of the native vascular regions. Using this model, we studied the effectors that regulate monocyte adhesion and migration, as well as foam cell formation inside vessel walls. We found that monocyte adhesion and migration are regulated by both the endothelium and monocytes themselves. Monocytes migrated into the extracellular matrix only when endothelial cells were cultured in the vessel model. In addition, the exposure of an endothelial layer to tumor necrosis factor α (TNF-α) and low shear stress both increased monocyte migration into the subendothelial space toward the matrix. Furthermore, we demonstrated the process of foam cell formation, 3 days after transmigration of peripheral blood mononuclear cells (PBMCs) into the vessel wall. We showed that pre-exposure of PBMCs to high shear rates increases their adhesion and migration through the TNF-α-treated endothelium but does not affect their capacity to form foam cells. The versatility of our model allows for mechanistic studies on foam cell formation under customized pathological conditions.
- Published
- 2023
- Full Text
- View/download PDF
49. Paradigm Shift or Business as Usual: The Reception and Implementation of the BYU-Idaho Learning Model by Faculty Members--A Mixed Methods Study
- Author
-
Thurgood, Larry L.
- Abstract
A mixed methods study examined how a newly developed campus-wide framework for learning and teaching, called the Learning Model, was accepted and embraced by faculty members at Brigham Young University-Idaho from September 2007 to January 2009. Data from two administrations of the Approaches to Teaching Inventory showed that (a) faculty members were more student-centered than teacher- centered in September 2007, and (b) there was a small, but statistically significant increase in student-centeredness in January 2009. Qualitative survey responses and faculty interviews expressed how the Learning Model had influenced teaching approaches and philosophy. Emergent themes included increased student preparation and responsibility for learning, the role of teaching one another, teacher improvement, and spiritual components of the Learning Model, as well as the view that there had been no change in teaching approaches. Overall, the Learning Model has been accepted and utilized by BYU-Idaho faculty members. [The dissertation citations contained here are published with the permission of ProQuest LLC. Further reproduction is prohibited without permission. Copies of dissertations may be obtained by Telephone (800) 1-800-521-0600. Web page: http://www.proquest.com/en-US/products/dissertations/individuals.shtml.]
- Published
- 2010
50. Mechanosensing by Piezo1 and its implications for physiology and various pathologies
- Author
-
Lai, A, Cox, CD, Sekar, NC, Thurgood, P, Jaworowski, A, Peter, K, Baratchi, S, Lai, A, Cox, CD, Sekar, NC, Thurgood, P, Jaworowski, A, Peter, K, and Baratchi, S
- Abstract
Piezo1 is a mechanosensitive ion channel with essential roles in cardiovascular, lung, urinary, and immune functions. Piezo1 is widely distributed in different tissues in the human body and its specific roles have been identified following a decade of research; however, not all are well understood. Many structural and functional characteristics of Piezo1 have been discovered and are known to differ greatly from the characteristics of other mechanosensitive ion channels. Understanding the mechanisms by which this ion channel functions may be useful in determining its physiological roles in various organ systems. This review provides insight into the signalling pathways activated by mechanical stimulation of Piezo1 in various organ systems and cell types. We discuss downstream targets of Piezo1 and the overall effects resulting from Piezo1 activation, which may provide insights into potential treatment targets for diseases involving this ion channel.
- Published
- 2022
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.