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Dissolved organic matter (DOM) is a key soil quality property, indicative of the organic matter stored in the soil, which may also be a function of temporal variation. This study examines whether DOM is a robust property of the soil, controlling fertility, or if it may change with time. Altogether eight sets of soil samples were collected in 2018 and 2019 from the cultivated topsoil (0–10 cm) of cropland and from a nearby grassland near Martonvásár, Hungary. The study sites were characterized by Chernozem soil and were part of a long-term experimental project comparing the effects of manure application and fertilization to the control under maize and wheat monocultures. DOM was extracted from the samples with distilled water. The dissolved organic carbon (DOC), total dissolved nitrogen (DN), biological index (BIX), fluorescence index (FI), humification index (HIX), carbon nitrogen (C/N) ratio and specific ultraviolet absorbance at 254 nm (SUVA254) index were studied in the arable soils, and the results showed that all the DOM samples were humified, suggesting relevant microbiological contributions to the decomposition of OM and its conversion into more complex molecules (FI = 1.2–1.5, BIX = ~0.5, and HIX = ~0.9). Temporal variations were detected only for the permanent grassland where higher DOM concentration was found in spring. This increased DOM content mainly originated from humified, solid phase associated, recalcitrant OM. In contrast, there were no differences among fertilization treatments and sampling dates under cropfield conditions. Moreover, climatic conditions were not proven as a general ruler of DOM properties. Therefore, momentary DOM alone is not necessarily the direct property of soil organic matter under cropfield conditions. The application of this measure needs further details of sampling conditions to achieve adequate comparability.