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Minh Hoang Thi,1 Chung Dang Thanh,2 Thuan Huynh Quang3 1Department of Pathophysiology, Vietnam Military Medical University (VMMU), Ha Noi City, Vietnam; 2Department of Pathology and Forensic Medicine, 103 Military Medical Hospital, Vietnam Military Medical University (VMMU), Ha Noi City, Vietnam; 3Biochemistry Department, 103 Military Medical Hospital, Vietnam Military Medical University (VMMU), Ha Noi City, VietnamCorrespondence: Thuan Huynh Quang, Biochemistry Department, 103 Military Medical Hospital, Vietnam Military Medical University (VMMU), No. 160, Phung Hung Street, Phuc La Ward, Ha Dong District, Hanoi City, Vietnam, Tel +84904175342, Email huynhquangthuan@vmmu.edu.vnPurpose: Angiopoietin-Like3 is a protein that plays an important role in regulating plasma triglyceride concentrations by inhibiting the enzyme lipoprotein lipase. Lipid metabolism and glucose metabolism are closely related and interact with each other. ANGPTL3 may also be a factor involved in blood glucose regulation through an increase in free fatty acids generated from enhanced lipolysis in adipose tissue leading to insulin resistance. This study aimed to investigate plasma ANGPTL3 concentrations and their correlation with lipid and glucose metabolic markers in newly diagnosed type 2 Diabetes Mellitus patients.Subject and Methods: A cross-sectional descriptive study was conducted on 98 healthy subjects (control group) and 103 patients with type 2 diabetes at the first diagnosis, without any treatment (patient group). Plasma ANGPTL3 concentration was quantified by the ELISA method. The study determines the correlation of ANGPTL3 concentration with some indicators reflecting lipid and glucose metabolism.Results: The concentration of ANGPTL3 in the newly diagnosed type 2 Diabetes Mellitus patient group was lower than in the control group, the difference was statistically significant with p < 0.05. In the patient group: there was an inverse correlation between ANGPTL3 concentration and HDL-C concentration (r = − 0.37; p< 0.001), and a positive correlation with triglyceride concentration (r = 0.275; p < 0.05). There was no correlation between plasma ANGPTL3 levels and anthropometric indices, total cholesterol, HDL-C, glucose, HbA1C, insulin, and HOMA-IR. In the control group: there was no correlation between ANGPTL3 and any of the indicators mentioned above.Conclusion: ANGPTL3 levels in newly diagnosed type 2 diabetes mellitus patients were statistically significantly lower than in healthy subjects. Plasma ANGPTL3 was positively correlated with triglyceride levels and inversely correlated with HDL-C levels in newly diagnosed type 2 Diabetes mellitus patients.Keywords: type 2 diabetes, ANGPTL3, HDL-C, triglyceride

Thuan Huynh Quang,1,2 Minh Pham Nguyet,3 Diep Pham Thao,4 Minh Hoang Thi,1,2 Lan Phuong Thi Dam,1,2 Hang Ho Thi,1,2 Anh Phan Van,1,2 Thang Can Luong,5 Mai Ngo Thi Tuyet,2 Quy Dang Duy,1,2 Binh Do Nhu,1,2 Thuan Nghiem Duc1,2 1Military Hospital 103, Ha Noi, Viet Nam; 2Vietnam Military Medical University, Ha Noi, Vietnam; 3 19-8 Hospital, Ministry of Public Security, Ha Noi, Vietnam; 4Viet Duc Hospital, Ha Noi, Viet Nam; 5Thanh Nhan Hospital, Ha Noi, Viet NamCorrespondence: Thuan Nghiem Duc Email nghiemthuan.hvqy@gmail.comPurpose: The purpose of this study was evaluating the early diagnostic value of two specific tubular markers neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) in diabetes nephropathy.Patients and Methods: Cross-sectional study was carried in three groups of patients from 10/2017 to 10/2018 in Military Hospital 103. Group I included 30 healthy peoples with estimated glomerular filtration rate (eGFR) > 60 mL/min/1.73 m2 and urine albumin creatinine ratio (uACR) < 30 mg/g. Group II included 30 type 2 diabetic patients having uACR < 30 mg/g, eGFR > 60 mL/min/1.73 m2. Group III included 30 type 2 diabetic patients having uACR > 30 mg/g, eGFR > 60 mL/min/1.73 m2.Results: Urine KIM-1 and NGAL increased progressively from control group (57.29 ± 25.91 pg/mL; 25.71 ± 13.69 ng/mL) to the group of diabetic patients with uACR < 30 mg/g (167.06 ± 44.01 pg/mL; 37.42 ± 10.89 ng/mL) and the group of diabetic patients with uACR ≥ 30 mg/g) (p < 0.05). There were moderate correlations between KIM-1 (r = 0.48, p < 0.05) and NGAL (r = 0.45, p < 0.05) with uACR. There was a mild correlation between KIM-1 and NGAL (r = 0.29, p < 0.05). KIM-1 and NGAL are the independent tests to detect diabetic nephropathy. The sensivity and specificity of KIM-1 with cut-off value of 174.95 pg/mL were 62.37% and 73.48%, respectively; the sensivity and specificity of NGAL with cut-off value of 35.2 ng/mL were 60.45% and 70.37%, respectively.Conclusion: KIM-1 and NGAL in urine are independent markers for early diagnostic diabetic nephropathy.Keywords: diabetic kidney disease, urine albumin creatinin ratio, albumin urine

Background: Chronic kidney disease (CKD) is an increasingly common disease worldwide and has become a global health problem, especially in Vietnam. Cystatin C is a marker for the detection, classification, and prognosis of CKD. Cystatin C is filtered entirely through the glomerular membrane, reabsorbed, and metabolized completely in the renal tubules. In case of damage to the kidneys, glomerular filtration rate declines, and some substances increase in the blood, such as cystatin C. The concentration of cystatin C changes with damage to the renal system. Objectives: This study aimed to estimate the concentration of cystatin C and its variation in the different stages of CKD. Methods: A descriptive, cross-sectional study was conducted on 40 healthy individuals and 137 patients with CKD grade III, IV, and V in 103 Hospital. The concentration of cystatin C was estimated in all subjects. Results: Cystatin C plasma levels were significantly higher in the CKD group (9.17 ± 3.75 mg/L) than in the control group (0.82 ± 0.12 mg/L). Cystatin C plasma levels increased linearly with the serious kidney failure as the stage of CKD. Conclusions: Cystatin C is an effective marker for estimating kidney damage in CKD.

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