108 results on '"Thorin C"'
Search Results
2. Effects of long-term active immunization with the second extracellular loop of human β1- or β3-adrenoceptors in thoracic aorta and mesenteric arteries in Lewis rats
- Author
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Montaudon, E., Dubreil, L., Lalanne, V., Jagu, B., Toumaniantz, G., Thorin, C., Henrion, D., Desfontis, J.-C., Martignat, L., and Mallem, M.Y.
- Published
- 2016
- Full Text
- View/download PDF
3. Effect of equilibration time on the motility and functional integrity of canine spermatozoa frozen in three different extenders
- Author
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Belala, R., Briand-Amirat, L., Vinciguerra, L., Tainturier, D., Kaidi, R., Thorin, C., Michaud, S., Anton, M., and Bencharif, D.
- Published
- 2016
- Full Text
- View/download PDF
4. Vasodilatory effect of pentoxifylline in isolated equine digital veins
- Author
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Kabbesh, N., Gogny, M., Chatagnon, G., Noireaud, J., Thorin, C., Desfontis, J.-C., and Mallem, M.Y.
- Published
- 2012
- Full Text
- View/download PDF
5. 82 Are antibiotics still truly needed in bovine embryo collection media? A preliminary study
- Author
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Marquet, M., primary, Ben Hania, W., additional, Thorin, C., additional, Guilbert-Julien, L., additional, Quinton, H., additional, Pol, J., additional, Escouflaire, P., additional, Chevrier, L., additional, Gard, J., additional, Chavatte-Palmer, P., additional, and Briand-Amirat, L., additional
- Published
- 2021
- Full Text
- View/download PDF
6. Synthetic peptide issued from Hap1/LipL32 for new early serodiagnosis of human leptospirosis
- Author
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Aviat, F., Rochereau-Roulet, S., Branger, C., Estavoyer, J.M., Chatrenet, B., Orsonneau, J.L., Thorin, C., and Andre-Fontaine, G.
- Published
- 2010
- Full Text
- View/download PDF
7. In vitro comparison of myometrial contractility induced by aglepristone-oxytocin and aglepristone-PGF2alpha combinations at different stages of the estrus cycle in the bitch
- Author
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Gogny, A., Mallem, Y., Destrumelle, S., Thorin, C., Desfontis, J.-C., Gogny, M., and Fiéni, F.
- Published
- 2010
- Full Text
- View/download PDF
8. A kinetic of effects study: an attempt to model intracellular mechanisms generated by drug-receptor interaction: 12.5.
- Author
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THORIN, C., MALLEM, Y., PINGUET, A., KANSO, H., and DESFONTIS, J.-C.
- Published
- 2015
9. Séroprévalence de la borréliose de Lyme et de l’encéphalite à tiques chez des professionnels exposés dans le Grand Est de la France
- Author
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Thorin, C., Rigaud, E., Capek, I., André-Fontaine, G., Oster, B., Gastinger, G., and Abadia, G.
- Published
- 2008
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10. Exposure of Raptors and Waterbirds to Anticoagulant Rodenticides (Difenacoum, Bromadiolone, Coumatetralyl, Coumafen, Brodifacoum): Epidemiological Survey in Loire Atlantique (France)
- Author
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Lambert, O., Pouliquen, H., Larhantec, M., Thorin, C., and L’Hostis, M.
- Published
- 2007
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- View/download PDF
11. Honeybees, honey and pollen as sentinels to monitor pesticides presence in environment: 15.8.
- Author
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LAMBERT, O., PUYO, S., THORIN, C., WIEST, L., BULETE, A., DELBAC, F., and POULIQUEN, H.
- Published
- 2012
12. 145 ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study
- Author
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Ruelle, I., primary, Seregeant, N., additional, Bencharif, D., additional, Charreaux, F., additional, Thorin, C., additional, Michaud, S., additional, Dordas-Perpinyà, M., additional, Jouy, N., additional, Audry, S., additional, Maurage, C., additional, Delehedde, M., additional, and Briand-Amirat, L., additional
- Published
- 2020
- Full Text
- View/download PDF
13. What Is the Effect of Anti-GnRH Immunization on Plasmatic Levels of Anti Mullerian Hormone?
- Author
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Dordas-Perpinya, M., Gorréguès, M., Gervasoni, M.-A., Berder, C., Thorin, C., Jaillardon, L., and Bruyas, J.-F.
- Published
- 2018
- Full Text
- View/download PDF
14. The Effect Of Soluble Guanylate Cyclase (Sgc) And Beta3-Adrenoceptor Activation On The Circulatory Function Of Watanabe Heritable Hyperlipidemic (Whhl) Rabbits
- Author
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Moughaizel, M., primary, Thorin, C., additional, Mallem, Y., additional, and Desfontis, J.C., additional
- Published
- 2019
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15. Systemic morphine administration causes gastric distention and hyperphagia in healthy horses
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Tessier, C., primary, Pitaud, J.‐P., additional, Thorin, C., additional, and Touzot‐Jourde, G., additional
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- 2019
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16. Recovery of insulin sensitivity and optimal body composition after rapid weight loss in obese dogs fed a high‐protein medium‐carbohydrate diet
- Author
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André, A., primary, Leriche, I., additional, Chaix, G., additional, Thorin, C., additional, Burger, M., additional, and Nguyen, P., additional
- Published
- 2017
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17. Etude ostéométrique des métapodes de chèvres (Capra hircus, L., 1758) d'Algérie : cas de la race autochtone Arabia.
- Author
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GUINTARD, C., RIDOUH, R., THORIN, C., and TEKKOUK-ZEMMOUCHI, F.
- Abstract
Copyright of Revue de Médecine Vétérinaire is the property of Ecole Nationale Veterinaire de Toulouse and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2018
18. In vitro bovine embryo production in a synthetic medium: Embryo development, cryosurvival, and establishment of pregnancy
- Author
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Moreno, D., primary, Neira, A., additional, Dubreil, L., additional, Liegeois, L., additional, Destrumelle, S., additional, Michaud, S., additional, Thorin, C., additional, Briand-Amirat, L., additional, Bencharif, D., additional, and Tainturier, D., additional
- Published
- 2015
- Full Text
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19. Étude ostéo-biométrique de la « race » cameline algérienne Sahraoui (Camelus dromedarius L., 1758)
- Author
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Adamou, A., TEKKOUK-ZEMMOUCHI, F., Thorin, C., BRERHI, E.H., Borvon, Aurélia, BABELHADJ, B., GUINTARD*, C., Université Kasdi Merbah Ouargla, Université Mentouri Constantine [Algérie] (UMC), Ecole Nationale Vétérinaire de Nantes, Archéologies et Sciences de l'Antiquité (ArScAn), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris Nanterre (UPN)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Université frères Mentouri Constantine I (UMC), and Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Ministère de la Culture et de la Communication (MCC)-Institut national de recherches archéologiques préventives (Inrap)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory ,metatarsal bone ,Osteometry ,biometry ,metacarpal bone ,race Sahraoui ,biométrie ,Camelus dromedarius ,dromadaire ,dromedary ,métatarse ,métacarpe ,Sahraoui breed ,Ostéométrie - Abstract
International audience; This study was realized on a sample of 60 dromedaries of Sahraoui breedfrom the south of Algeria. The animals, 30 males and 30 females, are adultsover 6 years destined for slaughter. Before slaughter in Ouargla abattoir,measurements during their life-time were performed (3 parameters).Carcass weight was recovered at the slaughter house. The canon bonesor metapodials were taken (one left metacarpus and metatarsus of eachanimal), were weighed and measured after cooking and drying (16 variablesper animal), gracility index of bones were calculated (6 indexes per animal).The variability and correlations between variables were analyzed and allowwhile realizing a referential of archeozoology, to consider the ability tograsp certain parameters of live animal from bone measurements. Linearregression equations were proposed in order to estimate the animal’s liveweight, and its height at the withers from the best correlated measurements.A multivariate function (logistic regression) from seven linear parametersof each metapodial was calculated, it permits to envisage sexing from anisolated bone. Sexual dimorphism appears clearly at the level of metapodial:the males have canon bones which are not only larger and stockier but alsolonger which is a remarkable difference in comparison to those encounteredin other ruminants like cattle or sheep for which the great length of bone isnot a dimorphic parameter.; Cette étude a été réalisée sur un échantillon de 60 dromadaires du sudalgérien, de race Sahraoui. Les animaux, 30 mâles et 30 femelles, sontdes adultes de plus de 6 ans destinés à la boucherie. Préalablement à leurabattage, à l’abattoir de Ouargla, des prises de mensurations de leur vivantont été réalisées (3 paramètres). Le poids de carcasse a ensuite été noté àl’abattoir. Les os canons, ou métapodes, ont été prélevés (un métacarpe etun métatarse gauches pour chaque animal) puis pesés et mesurés aprèscuisson et séchage (16 variables par animal). Des indices de gracilité desos ont été calculés (6 indices par animal). La variabilité et les corrélationsentre variables ont été analysées et permettent, notamment dans le cadre dela réalisation de référentiels pour l’archéozoologie, d’envisager de pouvoirappréhender certains paramètres de l’animal sur pied, à partir des mesuresdes os. Des équations de régression linéaire ont été proposées afin d’estimerle poids vif de l’animal et sa hauteur au garrot, à partir des mensurations lesmieux corrélées. Une fonction multivariée (régression logistique) à partirdes sept paramètres linéaires de chacun des métapodes a été calculée ; ellepermet d’envisager de sexer un os isolé. Le dimorphisme sexuel apparaît,en effet, au niveau du métapode de façon nette : les mâles ont des os canonnon seulement plus gros et plus trapus, mais aussi plus longs, ce qui est unedifférence notable par rapport à ce que l’on rencontre chez d’autres ruminantscomme les bovins ou les moutons pour lesquels la grande longueur de l’osn’est pas un paramètre dimorphique.
- Published
- 2013
20. Venous Valvular Distribution in the Thoracic and Pelvic Limbs of the Horse
- Author
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Harfoush, K., primary, Guintard, C., additional, Desfontis, J. C., additional, Thorin, C., additional, Douart, C., additional, Mallem, M. Y., additional, and Betti, E., additional
- Published
- 2015
- Full Text
- View/download PDF
21. In vivo fertility of bull semen following cryopreservation with an LDL (low density lipoprotein) extender: Preliminary results of artificial inseminations
- Author
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Amirat-Briand, Lamia, Bencharif, Djemil, Vera-Munoz, Oscar, Pineau, S., Thorin, C., Destrumelle, S., Anton, Marc, Jouan, M., Shmitt, E., TAINTURIER, D., Laboratory of Biotechnology and Pathology of Reproduction, Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), IMV Technologies, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Centre d'Insémination Artificielle la Crespelle, and Partenaires INRAE
- Subjects
fluids and secretions ,ARTIFICIAL INSEMINATION ,urogenital system ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,FERTILITY ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,FREEZING ,LDL EXTENDER ,SEMEN ,BULL - Abstract
International audience; A semen extender made with low density lipoproteins (LDL) has been used instead of a standard extender that is already available on the market for the cryopreservation of bovine semen. However, in order to extend its use to artificial insemination centres, in vivo fertility studies were required. Semen was taken from three bulls and frozen-thawed in two extenders: the LDL extender and a standard Tris-egg-yolk (20%) extender used by AI centres. The quality of the semen was assessed prior to artificial insemination: motility was assessed using an image analyser (Computer Assisted Semen Analysis (Hamilton Thorne)), and the integrity of the plasma membrane was assessed using the hypo-osmotic test (HOS test). For the first time, gestations were obtained following the artificial insemination of cows in the field (n = 193) with semen that had been frozen-thawed in the LDL extender. No significant difference (p > 0.05) was detected between the success rates of AI between the semen that had been frozen-thawed in the LDL extender (59.2%) and the control extender, Tris-20% egg yolk (65.3%). In conclusion, the in vivo fertility of semen that has been frozen-thawed in the LDL extender is maintained since gestations are obtained following AI
- Published
- 2010
22. Venous Valvular Distribution in the Thoracic and Pelvic Limbs of the Horse.
- Author
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Harfoush, K., Guintard, C., Desfontis, J. C., Thorin, C., Douart, C., Mallem, M. Y., and Betti, E.
- Subjects
VENOUS valves ,LAMINITIS ,HORSE diseases ,HYDROSTATIC pressure ,VASOCONSTRICTION ,DISEASES ,HORSES - Abstract
Dysfunction of venous valves can lead to hemodynamic disorders causing venous stasis, which would favour the occurrence of equine laminitis. However, very few studies have investigated venous valves in the horse digit. The purpose of this study was to compare valvular density between thoracic and pelvic limbs and to study the relationship between valvular density of veins and their location, diameter and wall thickness. After dissection, valvular density was calculated based on the number of valves counted in the principal veins of 7 thoracic and 7 pelvic limbs from 7 horses. Our results showed that the valvular density was higher in thoracic limbs, which probably reflects the adaptation to the consequences of hydrostatic pressure. The superficial veins have a higher valvular density that would prevent the varicose risk in the horse. The lower valvular density in the thick veins can be explained by the high density of the smooth muscular cells contained, which would cause an important vasoconstriction via the sympathetic nervous system. The veins with a large diameter also have a lower valvular density; these veins are not exposed to important changes in hydrostatic pressure. Other valvular characteristics may also be involved in the vascular disorders that may be related to the pathophysiology of laminitis. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
23. Étude ostéo-biométrique comparée des « races » camelines algériennes Sahraoui et Targui (Camelus dromedarius L., 1758).
- Author
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BABELHADJ, B., ADAMOU, A., THORIN, C., TEKKOUK-ZEMMOUCHI, F., BENAISSA, A., and GUINTARD, C.
- Abstract
Copyright of Revue de Médecine Vétérinaire is the property of Ecole Nationale Veterinaire de Toulouse and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2016
24. Influence of dietary protein content and source on colonic fermentative activity in dogs differing in body size and digestive tolerance1
- Author
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Nery, J., primary, Goudez, R., additional, Biourge, V., additional, Tournier, C., additional, Leray, V., additional, Martin, L., additional, Thorin, C., additional, Nguyen, P., additional, and Dumon, H., additional
- Published
- 2012
- Full Text
- View/download PDF
25. Nitrate and nitrite concentrations in rabbit saliva: Comparison with rat saliva
- Author
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Djekoun-Bensoltane, S., Kammerer, M., Larhantec, M., Pilet, N., and Thorin, C.
- Published
- 2007
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26. La axiomática y las aplicaciones de la matemática
- Author
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Borel, Emilio and Thorin C., L.
- Subjects
Axiomas ,definiciones geométricas ,aforismo de Russel - Abstract
Es, si no me equivoco, el ilustre filosofo ingles Bertrand Russel el que dio de la Matemática la definición algo humorística siguiente : "es la única ciencia en que nunca se sabe de lo que se habla, y en que siempre se ignora si lo que se dice es cierto". Esto quiere decir que se parte de axiomas y de definiciones arbitrarias y que la verdad de las conclusiones depende de las convenciones aceptadas respecto a esos axiomas y a esas definiciones. Las definiciones geométricas pueden ser las de la geometría Euclideana, 0 de la geometría de Lobatchevsky; se da así distinto sentido a las palabras: recta, plano, círculo , etc., y se llega a verdades diferentes.
- Published
- 1963
27. Cómo nació la carrera de estadístico en la Universidad Nacional de Colombia
- Author
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Thorin Casas Luis
- Subjects
Estadística ,Historia ,Universidad Nacional de Colombia ,Carrera de Estadística ,Statistics ,HA1-4737 - Published
- 1979
28. La estadística elemental como parte de la educación general
- Author
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Thorin Casas Luis
- Subjects
Enseñanza de la estadística ,educación secundaria ,Statistics ,HA1-4737 - Abstract
Si bien existe un consentimiento casi universal en los centros de alta enseñanza con relación al estudio de la estadística, no sucede lo mismo con respecto a la interesante iniciativa de trabajar para que se incluya también, dentro de las materias de la enseñanza secundaria, un curso elemental de estadística como parte integrante de la educación general. Vamos por tanto a tratar de contestar a las preguntas: Conviene o no incluir un curso de estadística en el bachillerato? En caso afirmativo: En que, específicamente, debe consistir ese curso?
- Published
- 1968
29. Gestational cholestyramine treatment protects adult offspring of ApoE-deficient mice against maternal-hypercholesterolemia-induced atherosclerosis.
- Author
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Habib M, Croyal M, Kaeffer B, Grit I, Castellano B, Gourdel M, Le May C, Thorin C, Nazih H, and Ouguerram K
- Abstract
Aim: Perinatal hypercholesterolemia exacerbates the development of atherosclerotic plaques in adult offspring. Here, we aimed to study the effect of maternal treatment with cholestyramine, a lipid-lowering drug, on atherosclerosis development in adult offspring of hypercholesterolemic ApoE-deficient (ApoE
-/- ) mice., Methods: ApoE-/- mice were treated with 3% cholestyramine (CTY) during gestation (G). After weaning, offspring (CTY-G) were fed control diet until sacrificed at 25weeks of age. Atherosclerosis development in the aortic root of offspring was assessed after oil-red-o staining, along with some of predefined atherosclerosis regulators such as LDL and HDL by high-performance liquid chromatography (HPLC), and bile acids (BA) and trimethylamine N-oxide (TMAO) by liquid chromatography-mass spectrometry (LC-MS/MS)., Results: In pregnant dams, cholestyramine treatment resulted in significantly lower plasma total- and LDL-cholesterol as well as gallbladder total BA levels. In offspring, both males and females born to treated dams displayed reduced atherosclerotic plaques areas along with less lipid deposition in the aortic root. No significant change in plasma total cholesterol or triglycerides was measured in offspring, but CTY-G males had increased HDL-cholesterol and decreased apolipoproteins B100 to A-I ratio. This latter group also showed reduced gallbladder total and specifically tauro-conjugated bile acid pools, whereas for CTY-G females, hydrophilic plasma tauro-conjugated BA pool was significantly higher. They also benefited from lower plasma TMAO., Conclusion: Prenatal cholestyramine treatment reduces atherosclerosis development in adult offspring of ApoE-/- mice along with modulating the plaques' composition as well as some related biomarkers such as HDL-C, bile acids and TMAO., (© 2024 The Authors. Acta Physiologica published by John Wiley & Sons Ltd on behalf of Scandinavian Physiological Society.)- Published
- 2024
- Full Text
- View/download PDF
30. Effects of chronic mirabegron treatment on metabolic and cardiovascular parameters as well as on atherosclerotic lesions of WHHL rabbits with high-fructose high-fat diet-induced insulin resistance.
- Author
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Moughaizel M, Dagher E, Bouhsina N, Lalanne V, Thorin C, Desfontis JC, and Mallem MY
- Subjects
- Acetanilides, Animals, Blood Glucose metabolism, Diet, High-Fat adverse effects, Fructose, Insulin, Rabbits, Thiazoles, Atherosclerosis metabolism, Insulin Resistance physiology
- Abstract
Background and Aim: Metabolic syndrome (MetS) is a global health and economic burden. Finding a suitable pharmacological approach for managing this syndrome is crucial. We explored the therapeutic potential of mirabegron (MIR), a β
3 -adrenergic receptor agonist, as a repurposed agent for the treatment of MetS and its cardiovascular consequences., Methods: Thirty Watanabe heritable hyperlipidemic rabbits (WHHL) were divided into 3 groups: control, high-fructose high-fat diet (HFFD) and HFFD + MIR that received a chow diet, HFFD and HFFD along with MIR treatment, respectively. The protocol lasted for 12 weeks, during which weight and abdominal circumference were monitored; plasma fasting levels of lipids, glucose and insulin were measured and an intravenous glucose tolerance test (IVGTT) was performed. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated. Cardiac function was assessed using in-vivo and ex-vivo approaches. Vascular reactivity was estimated via isolated carotid arteries method. Aortic atherosclerosis was evaluated using histological and immuno-histochemical techniques., Results: In contrast to the HFFD group, MIR-treated rabbits showed fasting insulin, HOMA-IR and TG levels stabilization and exhibited improved cardiac inotropy and lusitropy, while on the other hand, displayed aggravated atheroma plaque development., Conclusion: Long-term treatment with MIR prevented the increase in TG levels and the establishment of IR and enhanced the cardiac function of a rabbit animal model of MetS with combined dyslipidemia and IR., (Copyright © 2022. Published by Elsevier B.V.)- Published
- 2022
- Full Text
- View/download PDF
31. Long-term high-fructose high-fat diet feeding elicits insulin resistance, exacerbates dyslipidemia and induces gut microbiota dysbiosis in WHHL rabbits.
- Author
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Moughaizel M, Dagher E, Jablaoui A, Thorin C, Rhimi M, Desfontis JC, and Mallem Y
- Subjects
- Animals, Dysbiosis metabolism, Dyslipidemias metabolism, Female, Gastrointestinal Microbiome, Male, Rabbits, Diet, High-Fat adverse effects, Dysbiosis etiology, Dyslipidemias etiology, High Fructose Corn Syrup adverse effects, Insulin Resistance
- Abstract
The metabolic syndrome (MetS) has become a global public health burden due to its link to cardiovascular disease and diabetes mellitus. The present study was designed to characterize the metabolic and cardiovascular disturbances, as well as changes in gut microbiota associated with high-fructose high-fat diet (HFFD)-induced MetS in Watanabe heritable hyperlipidemic (WHHL) rabbits. Twenty-one Watanabe rabbits were assigned to a control (n = 9) and HFFD (n = 12) groups, receiving a chow diet and a HFFD, respectively. During a 12-weeks protocol, morphological parameters were monitored; plasma fasting levels of lipids, glucose and insulin were measured and a glucose tolerance test (GTT) was performed. HOMA-IR was calculated. Cardiac function and vascular reactivity were evaluated using the Langendorff isolated heart and isolated carotid arteries methods, respectively. 16S rRNA sequencing of stool samples was used to determine gut microbial composition and abundance. HFFD-fed Watanabe rabbits exhibited increased fasting insulin (p < 0.03, 12th week vs. Baseline), HOMA-IR (p < 0.03 vs. Control), area under the curve of the GTT (p < 0.02 vs. Control), triglycerides (p < 0.05, 12th week vs. Baseline), TC (p < 0.01 vs. Control), LDL-C (p < 0.001 vs. Control). The HFFD group also displayed a significant decrease in intestinal microbial richness, evenness and diversity (FDR < 0.001, FDR < 0.0001, FDR < 0.01, respectively vs. Control group) and an increase in its Firmicutes/Bacteroidetes ratio (R = 3.39 in control vs. R = 28.24 in the HFFD group) indicating a shift in intestinal microbial composition and diversity. Our results suggest that HFFD induces insulin resistance and gut microbiota dysbiosis and accentuates dyslipidemia; and that, when subjected to HFFD, Watanabe rabbits might become a potential diet-induced MetS animal models with two main features, dyslipidemia and insulin resistance., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2022
- Full Text
- View/download PDF
32. Comparison of Flunixin Meglumine, Meloxicam and Ketoprofen on Mild Visceral Post-Operative Pain in Horses.
- Author
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Lemonnier LC, Thorin C, Meurice A, Dubus A, Touzot-Jourde G, Couroucé A, and Leroux AA
- Abstract
The analgesic efficacy of meloxicam and ketoprofen against equine visceral pain is unclear. The aim of this study was to compare the analgesic efficacy of meloxicam (M) and ketoprofen (K) to flunixin meglumine (F) following inguinal castration. Horses undergoing inguinal castration under general anesthesia were randomly assigned F (1.1 mg/kg), M (0.6 mg/kg) or K (2.2 mg/kg) intravenously two hours pre-operatively and 24 h later. A pain score (out of 31) was recorded blindly by a senior clinician and veterinary student before NSAIDs administration (T
0 ), and after the first (T1 ) and second (T2 ) administrations, using a modified post-abdominal surgery pain assessment scale (PASPAS). Pain was classified as mild (score ≤ 7), moderate (score = 8-14) or severe (score > 14). Thirty horses (12 F, 10 M, 8 K) aged 6.2 ± 4.9 years, mostly warmbloods, were included. Horse welfare was not compromised regardless of the drug assigned. There was no statistically significant effect of NSAIDs on pain score. Mean pain scores were significantly higher at T1 than T0 for each NSAID (F: 5.08 ± 2.50 vs. 1.58 ± 1.38 ( p < 0.001); M: 4.60 ± 2.32 vs. 1.10 ± 1.20 ( p < 0.001); K: 5.25 ± 1.39 vs. 1.50 ± 1.51 ( p < 0.0001)) and lower at T2 than T1 for F (2.92 ± 2.423 vs. 5.08 ± 2.50 ( p < 0.001)) and M (2.90 ± 1.37 vs. 4.60 ± 2.32 ( p < 0.0325)). At T1 , senior pain scores were significantly different than for junior (5.56 ± 0.54 vs. 3.22 ± 0.62, p = 0.005). This study indicates that meloxicam and ketoprofen provide a similar level of analgesia to flunixin meglumine for the management of mild visceral pain in horses. PASPAS is not reliable for junior evaluators.- Published
- 2022
- Full Text
- View/download PDF
33. 82 Are antibiotics still truly needed in bovine embryo collection media? A preliminary study.
- Author
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Marquet M, Ben Hania W, Thorin C, Guilbert-Julien L, Quinton H, Pol J, Escouflaire P, Chevrier L, Gard J, Chavatte-Palmer P, and Briand-Amirat L
- Published
- 2021
- Full Text
- View/download PDF
34. Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces.
- Author
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Munir MT, Maneewan N, Pichon J, Gharbia M, Oumarou-Mahamane I, Baude J, Thorin C, Lepelletier D, Le Pape P, Eveillard M, Irle M, Pailhoriès H, Aviat F, Belloncle C, Federighi M, and Dubreil L
- Subjects
- Fluorescence, Quercus microbiology, Surface Properties, Triazines, Wood microbiology, Biofilms growth & development, Microscopy, Confocal, Spectrum Analysis, Staphylococcus aureus physiology
- Abstract
Methods to test the safety of wood material for hygienically sensitive places are indirect, destructive and limited to incomplete microbial recovery via swabbing, brushing and elution-based techniques. Therefore, we chose mCherry Staphylococcus aureus as a model bacterium for solid and porous surface contamination. Confocal spectral laser microscope (CSLM) was employed to characterize and use the autofluorescence of Sessile oak (Quercus petraea), Douglas fir (Pseudotsuga menziesii) and poplar (Populus euramericana alba L.) wood discs cut into transversal (RT) and tangential (LT) planes. The red fluorescent area occupied by bacteria was differentiated from that of wood, which represented the bacterial quantification, survival and bio-distribution on surfaces from one hour to one week after inoculation. More bacteria were present near the surface on LT face wood as compared to RT and they persisted throughout the study period. Furthermore, this innovative methodology identified that S. aureus formed a dense biofilm on melamine but not on oak wood in similar inoculation and growth conditions. Conclusively, the endogenous fluorescence of materials and the model bacterium permitted direct quantification of surface contamination by using CSLM and it is a promising tool for hygienic safety evaluation., (© 2021. The Author(s).)
- Published
- 2021
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35. Evaluation of the efficacy of enrofloxacin in rainbow trout (Oncorhynchus mykiss) following experimental challenge with Yersinia ruckeri.
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Rostang A, Peroz C, Fournel C, Thorin C, and Calvez S
- Subjects
- Administration, Oral, Animals, Treatment Outcome, Yersinia Infections drug therapy, Enrofloxacin therapeutic use, Fish Diseases drug therapy, Oncorhynchus mykiss microbiology, Yersinia Infections veterinary, Yersinia ruckeri
- Abstract
Background: Use of enrofloxacin in trout farms is reported, especially for the treatment of yersiniosis, albeit various dosing regimens have been used. Therefore, optimal doses should be investigated., Methods: Five groups of 15 fish were challenged with Y. ruckeri. Two days later, three groups received feed containing enrofloxacin (ENR) at 1, 2.5 and 5 mg/kg fish respectively, during 7 days; one group received a single intraperitoneal injection of ENR at 10 mg/kg; and one group was left untreated. On day 15, surviving fish were euthanized., Results: All fish survived in the group treated by injection, compared to 53%, 60% and 40% of the fish treated with 1, 2.5 and 5 mg/kg oral ENR, respectively, and 53% in the infected untreated group., Conclusion: A single intraperitoneal injection of ENR at 10 mg/kg seems more relevant than repeated oral administrations. The ENR oral doses used in trout farms should be revised., (© 2021 British Veterinary Association.)
- Published
- 2021
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36. Soluble guanylate cyclase chronic stimulation effects on cardiovascular reactivity in cafeteria diet-induced rat model of metabolic syndrome.
- Author
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Doghri Y, Dubreil L, Lalanne V, Hélissen O, Fleurisson R, Thorin C, Desfontis JC, and Mallem MY
- Subjects
- Animals, Aorta, Thoracic drug effects, Aorta, Thoracic enzymology, Aorta, Thoracic physiopathology, Cardiovascular Diseases enzymology, Cardiovascular Diseases etiology, Cardiovascular Diseases physiopathology, Coronary Circulation drug effects, Cyclic GMP metabolism, Disease Models, Animal, Enzyme Activation, Glucose Intolerance enzymology, Glucose Intolerance etiology, Glucose Intolerance physiopathology, Glucose Intolerance prevention & control, Hypertension enzymology, Hypertension etiology, Hypertension physiopathology, Hypertension prevention & control, Hypertriglyceridemia enzymology, Hypertriglyceridemia etiology, Hypertriglyceridemia physiopathology, Hypertriglyceridemia prevention & control, Isolated Heart Preparation, Male, Metabolic Syndrome enzymology, Metabolic Syndrome etiology, Metabolic Syndrome physiopathology, Nitric Oxide Synthase Type II metabolism, Obesity, Abdominal enzymology, Obesity, Abdominal etiology, Obesity, Abdominal physiopathology, Obesity, Abdominal prevention & control, Rats, Inbred SHR, Vasoconstriction drug effects, Vasodilation drug effects, Ventricular Function, Left drug effects, Ventricular Pressure drug effects, Rats, Cardiovascular Diseases prevention & control, Enzyme Activators pharmacology, Metabolic Syndrome prevention & control, Pyrazoles pharmacology, Pyridines pharmacology, Soluble Guanylyl Cyclase metabolism
- Abstract
Metabolic syndrome is linked to an increased risk of cardiovascular complications by a mechanism involving mainly decreased nitric oxide (NO) bioavailability and impaired NO-soluble guanylate cyclase (sGC)- cyclic guanosine monophosphate (cGMP) signalling (NO-sGC-cGMP). To further develop this scientific point, this study aimed to investigate the effects of long-term treatment with BAY 41-2272 (a sGC stimulator) on cardiovascular reactivity of spontaneously hypertensive rats (SHR) as a model of metabolic syndrome. SHR were randomly divided into 3 groups: control group, cafeteria diet (CD)-fed group and CD-fed group treated daily with BAY 41-2272 (5 mg/kg) by gastric gavage for 12 weeks. In vivo measurements of body weight, abdominal circumference, blood pressure and glucose tolerance test were performed. At the end of the feeding period, ex vivo cumulative concentration-response curves were performed on isolated perfused heart (isoproterenol (0.1 nM - 1 μM)) and thoracic aorta (phenylephrine (1 nM-10 μM), acetylcholine (1 nM-10 μM), and sodium nitroprusside (SNP) (0.1 nM-0.1 μM)). We showed that chronic CD feeding induced abdominal obesity, hypertriglyceridemia, glucose intolerance and exacerbated arterial hypertension in SHR. Compared to control group, CD-fed group showed a decrease in β-adrenoceptor-induced cardiac inotropy, in coronary perfusion pressure and in aortic contraction to phenylephrine. While relaxing effects of acetylcholine and SNP were unchanged. BAY 41-2272 long-term treatment markedly prevented arterial hypertension development and glucose intolerance, enhanced the α
1 -adrenoceptor-induced vasoconstriction, and restored cardiac inotropy and coronary vasodilation. These findings suggest that BAY 41-2272 may be a potential novel drug for preventing metabolic and cardiovascular complications of metabolic syndrome., (Copyright © 2021 Elsevier B.V. All rights reserved.)- Published
- 2021
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37. Plasma homocysteine concentration in privately owned healthy adult cats: assessment of biological determinants and establishment of a reference interval.
- Author
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Drut A, Soetart N, Bertin C, Le Corff E, Berder C, Thorin C, Nguyen P, Simard G, and Mallem MY
- Subjects
- Animals, Chromatography, High Pressure Liquid, Diet veterinary, Prospective Studies, Reference Values, Cats blood, Homocysteine blood
- Abstract
Objectives: The assessment of homocysteine status in diseased cats has indicated high plasma concentrations in chronic kidney disease and yielded conflicting results with respect to cardiovascular disorders. Previous investigations in small populations of normal cats revealed greater-than-expected variability in plasma homocysteine concentration. The purpose of this study was to determine biological determinants and the reference interval (RI) of plasma homocysteine concentration in the feline species, under strict pre-analytical conditions., Methods: In this prospective observational study, privately owned healthy adult cats underwent a complete physical examination, urinalysis and blood testing, in order to rule out any signs of disease. Plasma homocysteine concentration was measured using high-performance liquid chromatography-tandem mass spectrometry., Results: Of 151 cats recruited, 30 cats were not included owing to abnormal physical examination or fractious behaviour, and 30 cats were excluded based on abnormalities on blood work or urinalysis. Plasma homocysteine concentrations >28 µmol/l were associated with a dietary protein content >9.3 g/100 kcal metabolisable energy. The RI for plasma homocysteine concentration was determined to be 6.2-52.3 µmol/l., Conclusions and Relevance: Normal values for plasma homocysteine concentration in cats have a wide RI, suggesting high inter-individual variability. Whether some healthy cats exhibit impaired homocysteine metabolism remains to be elucidated.
- Published
- 2020
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38. Preliminary evaluation of an osteochondral autograft, a prosthetic implant, and a biphasic absorbable implant for osteochondral reconstruction in a sheep model.
- Author
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Olive M, Boyer C, Lesoeur J, Thorin C, Weiss P, Fusellier M, and Gauthier O
- Subjects
- Animals, Bone Regeneration, Calcium Phosphates, Female, Femur surgery, Stifle surgery, Transplantation, Autologous, Wound Healing, X-Ray Microtomography, Absorbable Implants, Autografts, Cartilage, Articular transplantation, Sheep surgery
- Abstract
Objective: To determine the ability of three implants to enhance the healing of osteochondral defects: (1) a biphasic construct composed of calcium phosphate (CaP) and chitosan/cellulosic polymer, (2) a titanium-polyurethane implant, and (3) an osteochondral autograft., Study Design: Experimental study., Animals: Ten adult female sheep., Methods: In five sheep, an 8-mm diameter osteochondral defect was created on the medial femoral condyle of a stifle and filled with a synthetic titanium-polyurethane implant. In five sheep, a similar defect was filled with an osteochondral autograft, and the donor site was filled with a biphasic construct combining CaP granules and a chitosan/cellulosic polymer. Sheep were monitored daily for lameness. Stifle radiographs and MRI were evaluated at 20 weeks, prior to animals being humanely killed. Surgical sites were evaluated with histology, microcomputed tomography, and scanning electron microscopy., Results: Clinical outcomes were satisfactory regardless of the tested biomaterials. All implants appeared in place on imaging studies. Osteointegration of prosthetic implants varied between sites, with limited ingrowth of new bone into the titanium structure. Autografts and biphasic constructs were consistently well integrated in subchondral bone. All autografts except one contained a cartilage surface, and all biphasic constructs except one partially restored hyaline cartilage surface., Conclusion: Biphasic constructs supported hyaline cartilage and subchondral bone regeneration, although restoration of the articular cartilage was incomplete., Clinical Impact: Biphasic constructs may provide an alternative treatment for osteochondral defects, offering a less invasive approach compared with autologous grafts and eliminating the requirement for a prosthetic implant., (© 2020 The Authors. Veterinary Surgery published by Wiley Periodicals, Inc. on behalf of American College of Veterinary Surgeons.)
- Published
- 2020
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39. Sildenafil citrate long-term treatment effects on cardiovascular reactivity in a SHR experimental model of metabolic syndrome.
- Author
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Doghri Y, Chetaneau F, Rhimi M, Kriaa A, Lalanne V, Thorin C, Maguin E, Mallem MY, and Desfontis JC
- Subjects
- Acetylcholine metabolism, Animals, Aorta, Thoracic drug effects, Aorta, Thoracic metabolism, Aorta, Thoracic microbiology, Blood Pressure drug effects, Cardiovascular Diseases metabolism, Cardiovascular Diseases microbiology, Cyclic GMP metabolism, Diet methods, Disease Models, Animal, Gastrointestinal Microbiome drug effects, Glucose Tolerance Test methods, Male, Metabolic Syndrome metabolism, Metabolic Syndrome microbiology, Phenylephrine metabolism, Rats, Rats, Inbred SHR, Vasodilation drug effects, Vasodilator Agents pharmacology, Cardiovascular Diseases drug therapy, Metabolic Syndrome drug therapy, Phosphodiesterase 5 Inhibitors pharmacology, Sildenafil Citrate pharmacology
- Abstract
Much evidence indicates that metabolic syndrome is strongly correlated with a decrease in nitric oxide and an increase in oxidative stress leading to cardiovascular alterations. In recent years, gut microbiota has emerged as a new contributor to the metabolic syndrome establishment and associated cardiovascular diseases, but the underlying mechanisms remain unclear. We hypothesized that a positive modulation of cyclic guanosine monophosphate (cGMP) pathway, through phosphodiesterase type 5 (PDE5) inhibition could prevent cardiovascular alterations and gut dysbiosis that may be associated to metabolic syndrome. Spontaneously hypertensive rats (SHR) were randomly divided into 4 groups: control, cafeteria diet (CD) and sildenafil citrate treated groups (5mg/kg per os) were given either a CD or a standard chow diet for 10 weeks. Body weight, arterial blood pressure and glucose tolerance test were monitored. At the 10th week, cardiac inotropy and coronary perfusion pressure were evaluated on isolated heart according to Langendorff method. Cumulative concentration response curves to phenylephrine and acetylcholine were determined on thoracic aorta rings for vascular reactivity evaluation. Faecal samples were collected for the gut microbiota analysis. Compared to the control group, CD-fed rats showed a significant increase in body weight gain, arterial blood pressure and were glucose intolerant. This group showed also a decrease in β-adrenoceptor-induced cardiac inotropy and coronary vasodilation. Gut microbiota analysis revealed a significant reduction in the abundance of Lactobocillus spp in cafeteria diet-fed rats when compared to the control ones. Sildenafil citrate long-term treatment decreased weight gain and arterial blood pressure, improved coronary vasodilation and reduced α1-adrenoceptor-induced vasoconstriction in CD group. However, it did not reverse gut dysbiosis induced by chronic CD feeding. These results suggest that cGMP pathway targeting may be a potential therapeutic strategy for the management of the metabolic syndrome and associated cardiovascular disorders., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2019
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40. A comparison of liquid and lyophilized egg yolk plasma to low density lipoproteins for freezing of canine spermatozoa.
- Author
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Belala R, Briand-Amirat L, Martinot A, Thorin C, Michaud S, Desherces S, Youngs CR, and Bencharif D
- Subjects
- Animals, Cryoprotective Agents pharmacology, Freeze Drying, Freezing, Male, Semen Analysis veterinary, Semen Preservation methods, Sperm Motility drug effects, Cryopreservation veterinary, Dogs, Egg Yolk chemistry, Lipoproteins, LDL pharmacology, Semen Preservation veterinary, Spermatozoa drug effects
- Abstract
The current study aimed to explore the potential usefulness of liquid or lyophilized egg yolk plasma (EYP) as a substitute for low-density lipoproteins (LDL) for cryopreservation of canine spermatozoa. In the first experiment, a total of 20 ejaculates harvested from six Beagles were frozen in extenders containing 6% LDL (control) or liquid or lyophilized EYP at one of three concentrations (20%, 40% or 60%). Motility parameters were assessed 10 min after thawing using computer-assisted sperm analysis. For both liquid and lyophilized EYP, the 40% concentration yielded motility similar (p > 0.05) to that observed with the control extender. In the second experiment, 12 ejaculates collected from the same six dogs were frozen in 6% LDL (Control), 40% liquid EYP or 40% lyophilized EYP extenders. Spermatozoal membrane integrity (hypo-osmotic swelling test [HOSt] and SYBR14/propidium iodide [PI] staining), acrosome integrity (FITC-Pisum sativum agglutinin staining) and DNA integrity (acridine orange staining) characteristics were evaluated 10 min after thawing. Both liquid and lyophilized 40% EYP-based extenders successfully preserved all assessed integrity parameters as efficiently as the control. Results of this study suggest that lyophilized EYP is a viable alternative to LDL in freezing extenders for dog semen., (© 2019 Blackwell Verlag GmbH.)
- Published
- 2019
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41. Mutations involved in the emergence of Yersinia ruckeri biotype 2 in France.
- Author
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Moreau E, Thomas T, Brevet M, Thorin C, Fournel C, and Calvez S
- Subjects
- Animals, Aquaculture, Bacterial Proteins genetics, Bacterial Typing Techniques veterinary, Fish Diseases epidemiology, France epidemiology, Mutation, Phenotype, Yersinia Infections epidemiology, Yersinia Infections microbiology, Yersinia ruckeri isolation & purification, Fish Diseases microbiology, Flagella genetics, Oncorhynchus mykiss microbiology, Yersinia Infections veterinary, Yersinia ruckeri genetics
- Abstract
Yersina ruckeri is an enterobacteria responsible for Enteric redmouth disease (ERM), which causes significant economic losses in the aquaculture industry worldwide. Two biotypes have been described within Y. ruckeri: biotype 1 (BT1) and biotype 2 (BT2). Unlike BT1, BT2 is negative for motility and lipase secretion. The emergence of BT2 Y. ruckeri has been associated with disease outbreaks in vaccinated fish in several countries, notably France in the early 2000s. In this study, 15 BT2 strains (14 BT2 strains isolated in France and the BT2 reference strain EX5) were studied to compare the phenotypic characters of the BT1 and BT2 strains and to determine the genetic origin of the emergence of BT2 in France. BT1 bacteria are significantly longer in size than BT2 bacteria (a difference of 0.222 µm). The loss of motility of some French BT2 strains could be due to the loss of their ability to produce flagella caused by three mutations within the fliG, flhC and flgA genes. In the light of these results, the emergence of BT2 Yersinia ruckeri in France is discussed., (© 2019 Blackwell Verlag GmbH.)
- Published
- 2019
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42. Comparison of morphological changes and tactile sensitivity of the pharynx and larynx between four standing sedative and analgesic protocols in eight adult healthy horses.
- Author
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Manneveau G, Lecallard J, Thorin C, Pamela H, and Tessier C
- Subjects
- Analgesia methods, Anesthesia methods, Anesthesia veterinary, Animals, Deep Sedation methods, Larynx anatomy & histology, Larynx drug effects, Pharynx anatomy & histology, Pharynx drug effects, Posture, Analgesia veterinary, Deep Sedation veterinary, Horses physiology, Larynx physiology, Pharynx physiology
- Abstract
Objective: To compare the topographic modifications and tactile sensitivity of the pharynx and larynx after administration of four sedative and analgesic protocols in standing horses., Study Design: Experimental, observer-blinded, crossover study., Animals: Eight healthy mares., Methods: Five protocols were evaluated: 1) xylazine and butorphanol administered intravenously (IV); 2) detomidine and butorphanol administered IV; 3) xylazine administered IV and lidocaine topically; 4) detomidine administered IV and lidocaine topically and 5) no analgesia or sedation (control). Quality of sedation, head height and sudden head movements were recorded. The degree of arytenoid cartilage displacement, the degree of pharyngeal collapse and the occurrence of soft palate displacement were scored using standardized scales. Tactile sensitivity was tested on 10 different pharyngeal and laryngeal regions using an atraumatic transendoscopic probe. Statistical analysis was performed using linear or generalized mixed-effects models., Results: Head height was significantly decreased in protocols with xylazine (p = 0.002). Head movements were significantly increased in protocols with butorphanol (p = 0.0001). No changes in abduction grade or degree of soft palate displacement were observed between all sedative protocols and the control group. Pharyngeal collapse was significantly more frequent in protocols with lidocaine (p < 0.001) or xylazine (p = 0.017). For the pharyngeal regions, no tactile sensitivity difference was observed between the control and treatment protocols. All treatment protocols led to greater desensitization of all the laryngeal regions compared with the control protocol., Conclusion and Clinical Relevance: All the protocols provided adequate sedation and analgesia for the manipulation of the larynx and pharynx but significant differences were noted. Xylazine produces a more profound sedation compared with detomidine, but can induce dorsal pharyngeal collapse. Lidocaine caused pharyngeal collapse and its use should be limited to the target area. Butorphanol can be added to improve analgesia in the other regions but frequent head jerking can be expected., (Copyright © 2018 Association of Veterinary Anaesthetists and American College of Veterinary Anesthesia and Analgesia. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2018
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43. Vascular Delivery of Allogeneic MuStem Cells in Dystrophic Dogs Requires Only Short-Term Immunosuppression to Avoid Host Immunity and Generate Clinical/Tissue Benefits.
- Author
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Lorant J, Larcher T, Jaulin N, Hedan B, Lardenois A, Leroux I, Dubreil L, Ledevin M, Goubin H, Moullec S, Deschamps JY, Thorin C, André C, Adjali O, and Rouger K
- Subjects
- Allogeneic Cells immunology, Animals, Dogs, Dystrophin immunology, Male, Muscular Dystrophy, Animal immunology, Stem Cells cytology, Stem Cells immunology, Transplantation, Homologous methods, Dog Diseases therapy, Immunosuppression Therapy methods, Muscular Dystrophy, Animal therapy, Stem Cell Transplantation methods
- Abstract
Growing demonstrations of regenerative potential for some stem cells led recently to promising therapeutic proposals for neuromuscular diseases. We have shown that allogeneic MuStem cell transplantation into Golden Retriever muscular dystrophy (GRMD) dogs under continuous immunosuppression (IS) leads to persistent clinical stabilization and muscle repair. However, long-term IS in medical practice is associated with adverse effects raising safety concerns. Here, we investigate whether the IS removal or its restriction to the transplantation period could be considered. Dogs aged 4-5 months old received vascular infusions of allogeneic MuStem cells without IS (GRMD
MU/no-IS ) or under transient IS (GRMDMU/tr-IS ). At 5 months post-infusion, persisting clinical status improvement of the GRMDMU/tr-IS dogs was observed while GRMDMU/no-IS dogs exhibited no benefit. Histologically, only 9-month-old GRMDMU/tr-IS dogs showed an increased muscle regenerative activity. A mixed cell reaction with the host peripheral blood mononucleated cells (PBMCs) and corresponding donor cells revealed undetectable to weak lymphocyte proliferation in GRMDMU/tr-IS dogs compared with a significant proliferation in GRMDMU/no-IS dogs. Importantly, any dog group showed neither cellular nor humoral anti-dystrophin responses. Our results show that transient IS is necessary and sufficient to sustain allogeneic MuStem cell transplantation benefits and prevent host immunity. These findings provide useful critical insight to designing therapeutic strategies.- Published
- 2018
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44. Skeletal Muscle Regenerative Potential of Human MuStem Cells following Transplantation into Injured Mice Muscle.
- Author
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Lorant J, Saury C, Schleder C, Robriquet F, Lieubeau B, Négroni E, Leroux I, Chabrand L, Viau S, Babarit C, Ledevin M, Dubreil L, Hamel A, Magot A, Thorin C, Guevel L, Delorme B, Péréon Y, Butler-Browne G, Mouly V, and Rouger K
- Subjects
- Adult Stem Cells, Animals, Cell Differentiation, Cell Proliferation, Cells, Cultured, Humans, Mice, Muscle Development, Muscular Dystrophy, Animal therapy, Muscular Dystrophy, Duchenne therapy, Regenerative Medicine, Muscle, Skeletal physiology, Myoblasts, Skeletal cytology, Myoblasts, Skeletal transplantation, Regeneration, Stem Cell Transplantation
- Abstract
After intra-arterial delivery in the dystrophic dog, allogeneic muscle-derived stem cells, termed MuStem cells, contribute to long-term stabilization of the clinical status and preservation of the muscle regenerative process. However, it remains unknown whether the human counterpart could be identified, considering recent demonstrations of divergent features between species for several somatic stem cells. Here, we report that MuStem cells reside in human skeletal muscle and display a long-term ability to proliferate, allowing generation of a clinically relevant amount of cells. Cultured human MuStem (hMuStem) cells do not express hematopoietic, endothelial, or myo-endothelial cell markers and reproducibly correspond to a population of early myogenic-committed progenitors with a perivascular/mesenchymal phenotypic signature, revealing a blood vessel wall origin. Importantly, they exhibit both myogenesis in vitro and skeletal muscle regeneration after intramuscular delivery into immunodeficient host mice. Together, our findings provide new insights supporting the notion that hMuStem cells could represent an interesting therapeutic candidate for dystrophic patients., (Copyright © 2017 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.)
- Published
- 2018
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45. Higher neonatal growth rate and body condition score at 7 months are predictive factors of obesity in adult female Beagle dogs.
- Author
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Leclerc L, Thorin C, Flanagan J, Biourge V, Serisier S, and Nguyen P
- Subjects
- Animals, Animals, Newborn, Dog Diseases metabolism, Dogs, Energy Intake, Energy Metabolism, Female, Longitudinal Studies, Obesity etiology, Obesity metabolism, Physical Conditioning, Animal, Risk Factors, Dog Diseases etiology, Obesity veterinary
- Abstract
Background: The risks during early growth on becoming overweight in adulthood are widely studied in humans. However, early-life predictive factors for canine adult overweight and obesity have not yet been studied. To identify factors that may help explain the development of overweight and obesity at adulthood in dogs, a longitudinal study of 2 years was conducted in 24 female Beagle dogs of the same age, sexual status, and raised under identical environmental conditions. By means of a hierarchical classification on principal components with the following quantitative values: fat-free mass (FFM), percentage fat mass and pelvic circumference at 2 years of age, three groups of dogs were established and were nominally named: ideal weight (IW, n = 9), slightly overweight (OW1, n = 6) and overweight (OW2, n = 9). With the aim of identifying predictive factors of development of obesity at adulthood parental characteristics, growth pattern, energy balance and plasma factors were analysed by logistic regression analysis., Results: At 24 months, the group compositions were in line with the body condition scores (BCS 1-9) values of the IW (5 or 6/9), the OW1 (6/9) and the OW2 (7 or 8/9) groups. Logistic regression analysis permitted the identification of neonatal growth rate during the first 2 weeks of life (GR
2W ) and BCS at 7 months as predictors for the development of obesity at adulthood. Seventy percent of dogs with either GR2W >125% or with BCS > 6/9 at 7 months belonged to the OW2 group. Results from energy intake and expenditure, corrected for FFM, showed that there was a greater positive energy imbalance between 7 and 10 months for the OW2, compared to the IW group., Conclusion: This study expands the understanding of previously reported risk factors for being overweight or obese in dogs, establishing that (i) 15 out of 24 of the studied dogs became overweight and (ii) GR2W and BCS at 7 months of age could be used as predictive factors as overweight adult dogs in the OW2 group had higher values compared the other groups of dogs.- Published
- 2017
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46. Gait characterization in golden retriever muscular dystrophy dogs using linear discriminant analysis.
- Author
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Fraysse B, Barthélémy I, Qannari EM, Rouger K, Thorin C, Blot S, Le Guiner C, Chérel Y, and Hogrel JY
- Subjects
- Accelerometry instrumentation, Age Factors, Animals, Clinical Decision-Making methods, Discriminant Analysis, Disease Models, Animal, Disease Progression, Dogs, Genotype, Linear Models, Male, Muscular Dystrophy, Animal genetics, Muscular Dystrophy, Duchenne genetics, Phenotype, Sensitivity and Specificity, Treatment Outcome, Accelerometry statistics & numerical data, Gait drug effects, Gait physiology, Immunosuppressive Agents therapeutic use, Muscular Dystrophy, Animal drug therapy, Muscular Dystrophy, Animal physiopathology, Muscular Dystrophy, Duchenne drug therapy, Muscular Dystrophy, Duchenne physiopathology
- Abstract
Background: Accelerometric analysis of gait abnormalities in golden retriever muscular dystrophy (GRMD) dogs is of limited sensitivity, and produces highly complex data. The use of discriminant analysis may enable simpler and more sensitive evaluation of treatment benefits in this important preclinical model., Methods: Accelerometry was performed twice monthly between the ages of 2 and 12 months on 8 healthy and 20 GRMD dogs. Seven accelerometric parameters were analysed using linear discriminant analysis (LDA). Manipulation of the dependent and independent variables produced three distinct models. The ability of each model to detect gait alterations and their pattern change with age was tested using a leave-one-out cross-validation approach., Results: Selecting genotype (healthy or GRMD) as the dependent variable resulted in a model (Model 1) allowing a good discrimination between the gait phenotype of GRMD and healthy dogs. However, this model was not sufficiently representative of the disease progression. In Model 2, age in months was added as a supplementary dependent variable (GRMD_2 to GRMD_12 and Healthy_2 to Healthy_9.5), resulting in a high overall misclassification rate (83.2%). To improve accuracy, a third model (Model 3) was created in which age was also included as an explanatory variable. This resulted in an overall misclassification rate lower than 12%. Model 3 was evaluated using blinded data pertaining to 81 healthy and GRMD dogs. In all but one case, the model correctly matched gait phenotype to the actual genotype. Finally, we used Model 3 to reanalyse data from a previous study regarding the effects of immunosuppressive treatments on muscular dystrophy in GRMD dogs. Our model identified significant effect of immunosuppressive treatments on gait quality, corroborating the original findings, with the added advantages of direct statistical analysis with greater sensitivity and more comprehensible data representation., Conclusions: Gait analysis using LDA allows for improved analysis of accelerometry data by applying a decision-making analysis approach to the evaluation of preclinical treatment benefits in GRMD dogs.
- Published
- 2017
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47. Effect of nebivolol treatment during pregnancy on the intrauterine fetal growth, mortality and pup postnatal development in the l-NAME-induced hypertensive rats.
- Author
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Altoama K, Mallem MY, Thorin C, Betti E, and Desfontis JC
- Subjects
- Animals, Animals, Newborn, Blood Pressure drug effects, Body Weight drug effects, Capillaries drug effects, Capillaries pathology, Female, Heart Rate drug effects, Hypertension physiopathology, Nebivolol therapeutic use, Pregnancy, Pregnancy Complications physiopathology, Rats, Rats, Wistar, Uterus blood supply, Uterus drug effects, Fetal Development drug effects, Hypertension chemically induced, Hypertension drug therapy, NG-Nitroarginine Methyl Ester pharmacology, Nebivolol pharmacology, Pregnancy Complications chemically induced, Pregnancy Complications drug therapy
- Abstract
The present study was carried out to evaluate the effect of nebivolol vs. bisoprolol treatment on the intrauterine fetal growth, mortality and postnatal development in N
ω -Nitro-l-arginine methyl ester hydrochloride (l-NAME)-induced hypertensive rats. Hypertension was induced in normotensive pregnant Wistar rats by daily administration of l-NAME (100mg/kg/day, in the drinking water) for the period of pregnancy. After 9 days of l-NAME treatment, rats with systolic and diastolic blood pressure (SBP and DBP) more than 140/90mmHg were considered hypertensive. Then, some of them were treated from day 11 to day 18 of pregnancy with nebivolol (8mg/kg/day) or bisoprolol (10mg/kg/day) via oral gavage. SBP, DBP and heart rate (HR) were re-evaluated by tail cuff method on day 19 of pregnancy and morphometrical or histological studies were performed on day 20. In addition, the mortality and postnatal development of newborn pups were assessed in all groups. The l-NAME administration during pregnancy induced an increase in SBP and DBP while HR did not change. Nebivolol or bisoprolol treatment completely prevented the elevation of SBP and DBP induced by l-NAME with a reduction in HR in pregnant and non-pregnant rats. The intra-uterine fetal growth and the postnatal development of newborn rats in nebivolol-treated hypertensive group were significantly lower vs. control and higher vs. bisoprolol-treated group with a higher mortality in the both types of treatments vs. control rats. The nebivolol and bisoprolol administration produce adverse effects on fetal growth and postnatal development, that limits their therapeutic use in females during pregnancy., (Copyright © 2016 Elsevier B.V. All rights reserved.)- Published
- 2016
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48. The benefits of liposomes for chilling canine sperm for 4 days at 4°C.
- Author
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Belala R, Delay J, Amirat L, Ropers MH, Guillou JL, Anton M, Schmitt E, Thorin C, Michaud S, Kaidi R, Tainturier D, and Bencharif D
- Subjects
- Animals, Dogs, Male, Refrigeration methods, Refrigeration veterinary, Semen physiology, Semen Preservation methods, Liposomes therapeutic use, Semen Preservation veterinary, Spermatozoa physiology
- Abstract
This study comprises 3 experiments exploring the possible benefits and mechanism of action of liposomes for chilling (4°C) canine sperm over a period of 4 days. In the first experiment, 20 ejaculates collected from 5 Beagle dogs were chilled in an extender containing 6% low density lipoproteins (LDL) (Control), or one of 7 extenders containing different concentrations (2, 4, 6, 8, 10, 15, 20%) of liposomes (LIPO). These ejaculates were chilled over 4 days and motility was assessed daily using a Hamilton Thorne analyzer (HTM-IVOS, 14.0). The 2% LIPO obtained the best results (p=0.038) after four days (72.55% motile spermatozoa and 31.4% progressive spermatozoa). In experiment 2, 10 ejaculates were collected from same 5 dogs and chilled in 6% LDL or 2% LIPO-based extenders. Sperm integrity characteristics were assessed prior to refrigeration and every 48h for four days (D0, D2, and D4). Acrosome integrity was assessed using the FITC-PSA test (Fluorescein IsoThiocyanate-Pisum Sativum Agglutinin), plasma membrane (PM) integrity using both the hypo-osmotic swelling test (HOSt) and SYBR14/Propidium Iodide test (SYBR14/PI), and DNA integrity using the Acridine-Orange test (AO). The 2% LIPO extender provided equivalent preservation of sperm integrity parameters to the reference extender (6% LDL). In experiment 3, a Langmuir-Blodgett trough was used to evaluate the mechanistic interactions between LDL, LIPO, prostatic fluid, and the canine spermatozoal membrane during chilling. Results indicate that LDL and LIPO interact differently with the biomimetic membrane. The most likely conclusion of these findings is that LDL and liposomes employ different protective mechanisms during the chilling (4°C) of canine spermatozoa., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
49. Risk assessment of proteolytic Clostridium botulinum in canned foie gras.
- Author
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Membré JM, Diao M, Thorin C, Cordier G, Zuber F, and André S
- Subjects
- Botulism prevention & control, Clostridium isolation & purification, Clostridium physiology, Clostridium botulinum isolation & purification, Clostridium botulinum physiology, France epidemiology, Hot Temperature, Humans, Microbial Viability, Models, Statistical, Risk Assessment, Spores, Bacterial isolation & purification, Botulism epidemiology, Food Handling standards, Food Microbiology
- Abstract
In this study, a risk assessment of proteolytic Clostridium botulinum in canned foie gras was performed, the number of illnesses per year in France due to C. botulinum in foie gras was estimated. Data on initial level in raw materials were collected at manufacturers and analysed using a Negative Binomial distribution. The effect of the usual foie gras heat treatment (equivalent time at 121 °C: F0=0.5 min) was considered at two levels: first, it led to an inactivation (estimated to 2.3 log); second it led to a spore injury and then to a spore inhibition. This latter effect was assessed by analysing data from a challenge test study carried out with Clostridium sporogenes spores in the foie gras product. The probability of spore recovering after thermal inhibition was estimated to 9.5×10(-8) (corresponding to 7.0 log). The data on the consumption pattern were collected on the French market. The Quantitative Microbiological Risk Assessment (QMRA) model and all the assumptions are reported in detail in the study. The initial contamination of raw materials, effect of thermal treatment on microbial inactivation and spore inhibition were handled mathematically using a probabilistic framework, considering only the variability dimension. The model was implemented in Excel and run through Monte Carlo simulation, using @Risk software. In parallel, epidemiological data collected from the French Institute for Public Health Surveillance during the period 2001-2012 were used to estimate an Appropriate Level Of Protection (ALOP) and then a Food Safety Objective (FSO): ALOP equalled to 2.5×10(-3) illnesses per million inhabitant per year, FSO equalled to 1.4×10(-9) foie gras portions containing C. botulinum spore (expressed in decimal logarithm, FSO=-8.9). The QMRA model output values were smaller, but on the same order of magnitude as these two figures: 8.0×10(-4) illnesses per million inhabitants per year, and, 4.5×10(-10) (-9.3 log) foie gras portions containing C. botulinum spores able to recover. It was then possible to conclude that the current practices regarding thermal treatment of canned foie gras are sufficient to control the risk of C. botulinum in foie gras in France., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
50. Effect of nebivolol treatment during pregnancy on the genital circulation, fetal growth and postnatal development in the Wistar rat.
- Author
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Altoama K, Yassine Mallem M, Thorin C, Betti E, and Desfontis JC
- Subjects
- Adrenergic beta-1 Receptor Antagonists pharmacology, Animals, Bisoprolol adverse effects, Bisoprolol pharmacology, Blood Pressure drug effects, Blood Pressure physiology, Body Weight drug effects, Female, Fetal Development physiology, Heart Rate drug effects, Heart Rate physiology, Pregnancy, Rats, Wistar, Regional Blood Flow physiology, Uterus blood supply, Uterus drug effects, Fetal Development drug effects, Genitalia blood supply, Nebivolol adverse effects, Nebivolol pharmacology, Prenatal Exposure Delayed Effects physiopathology, Regional Blood Flow drug effects
- Abstract
The aim of study was to evaluate the effects of nebivolol, a cardioselective beta-1 adrenergic receptor blocker of the third generation with vasodilatory properties, vs. bisoprolol on the genital circulation, uterine vasculature, fetal growth and postnatal development in pregnant Wistar rats. Non invasive measurements of systolic and diastolic blood pressure (SBP and DBP) and heart rate (HR), and invasive measurement of genital blood flow (GBF) were taken in pregnant rats, by tail cuff and transonic probe methods respectively, after an oral treatment by gastric gavage with nebivolol (8mg/kg/day) or bisoprolol (10mg/kg/day) from day 11 to day 18 of pregnancy. Other morphometrical and histological measurements were performed on the ovarian and uterine arteries to evaluate the effect of nebivolol on the uterine vasculature. Furthermore, postnatal mortality and pup growth were recorded. The data demonstrated that nebivolol (compared with bisoprolol) induced a significant decrease in SBP, HR and GBF while DBP remained unchanged. Moreover, nebivolol increased the diameter and the length of ovarian and uterine arteries and the number of uterine artery segmental branches. The results also showed that the body weight gain of newborns in the nebivolol group was significantly lower vs. bisoprolol and vs. control with a higher mortality rate. The nebivolol action is not only limited to its favorable hemodynamic effects represented by a decrease in blood pressure, but it also produces adverse effects on fetal growth and postnatal development that may limit its therapeutic use in females during pregnancy., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
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