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Abstract Palaeoparasitology investigates parasitological infections in animals and humans of past distance by examining biological remains. Palaeofaeces (or coprolites) are biological remains that provide valuable information on the disease, diet, and population movements in ancient times. Today, advances in detecting ancient DNA have cast light on dark corners that microscopy could never reach. The archaeological site of the Chehrabad salt mine of Achaemenid (550–330 BC) and Sassanid (third–seventh century AD) provides remains of various biotic and abiotic samples, including animal coprolites, for multidisciplinary studies. In the present work, we investigated coprolites for helminth eggs and larvae by microscopy and traced their biological agents’ DNA by Next Generation Sequencing. Our results revealed various helminths, including Taenia asiatica, the species introduced in the 1990s. Implementing advanced modern molecular techniques like NGS gives a paramount view of pathogenic agents in space and time.

Background: The ancient Chehrabad Salt mine, a well-known archaeological site in Iran, has recently received increasing interest from Iranian and international archeologists. Also, the biological remains from this site have provided valuable sources for studying the pathogenic agents of ancient times. This study aimed to identify the parasitic helminth eggs preserved in the herbivores coprolites. Methods: From 2011 to 2015, we received three coprolites belonging to herbivorous animals recovered during excavations in Chehrabad Salt mine of Zanjan, Iran. The coprolites were dated back to the Sassanid era (224-651 AD) by using radiocarbon accelerator mass spectrometry (AMS) and archeological stratigraphy methods. Following rehydration of the specimens in a 0.5% trisodium phosphate solution, the suspensions were mounted in glycerin jelly on glass slides and examined by a light microscope with 100x and 400x magnifications. Results: Two coprolites belonged to donkeys and one to an unknown herbivore species. The recovered eggs belonged to members of two helminths families, Strongylidae, and Anoplocephalidae. Also, within the two coprolites, some mites, presumably of the order Oribatida, were observed. Conclusion: The presence of two different nematodes in the equids coprolites provide clues of the burden of helminths infection on working animal at the Sassanid time and demonstrates the appropriate preservation condition of biological remains in the ancient salt mine of Chehrabad as well.

Present paper is the second publication introducing the paleoparasitological findings from animal coprolites obtained from archeological site of Chehrabad salt mine in northwest-ern Iran. The current archeological site is located in northwest of Iran , dated to the Sas-sanian Era (4th/5th century CE).In the summer 2012 the carnivore coprolite was obtained within the layers in the mine and were thoroughly analyzed for parasites using TSP re-hydration technique. Eggs of Macracanthorhynchus hirudinaceuswere successfully retrieved from the examined coprolite and were confidently identified based on reliable references. Identifying of M.hirudinaceuseggs in paleofeces with clear appearance as demonstrated herein, is much due to appropriate preservation condition has been existed in the salt mine .The present finding could be regarded as the oldest acanthocephalan infection in Iran.

Background: In this paper, paleoparasitological findings from rodent excrements ob-tained from Chehrabad Salt Mine archeological site located in northwest of Iran are demonstrated and discussed. Methods: Chehrabad Salt Mine archeological site located in northwest of Iran, dated to the Achaemenid (mid 1st mill. BCE) and to Sassanid (3rd cent. - 7th cent. CE) period, is a unique study area to investigate parasites in the past millenniums in Iran. Rodent cop-rolites obtained from this archeological site were thoroughly analyzed for parasite eggs using TSP re-hydration technique. Results: Specimen analyzed were attributed to juvenile and adult rats based on their apparent morphology comparing with the modern dried pellets of Muridea family. Hel-minth eggs retrieved from two positive pellets were identified as Trichosomoides crassi-cauda, Syphacia sp. and Trichuris sp. Conclusion: The present paper discusses the first paleoparasitological findings of ro-dent gastrointestinal helminthes in Iran along with possible favorite items to rats in ancient Chehrabad Salt Mine.

Computed tomography studies and histological analyses were performed on the mummified remains found in the Chehrābād salt mine in northwestern Iran. The ancient salt mummies are dated to the Achaemenid (550-330 BC) and Sassanid (3rd-7th century AD) time period and died in mining incidents. The aim of the study was to describe the radiological and histological findings of several ancient Iranian salt mummies with special interest in pathological and postmortem changes. The mummified remains show multiple traumatic alterations, such as fractures and signs of massive compression. Histological analyses can clearly differentiate soft tissue, however the preservation status is variable. These Iranian salt mummies are a rare example of the ancient Iranian population. The soft tissue and organs are well preserved, however in different degrees due to the varying conditions.