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11 results on '"Thomas Deshayes"'

1. Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison

Author

Anais Desoutter, Thomas Deshayes, Fabien Vorimore, Bernice Klotoe, Benoit Durand, Julien Colot, Gabriel Wagner-Lichtenegger, Ivo Steinmetz, Apichai Tuanyok, and Karine Laroucau

Subjects
Burkholderia pseudomallei, New Caledonia, Goat, Serology, ELISA, Luminex, Veterinary medicine, SF600-1100
Abstract

Abstract Background Melioidosis is a serious bacterial infection caused by Burkholderia pseudomallei, a gram-negative bacterium commonly found in soil and water. It can affect both humans and animals, and is endemic in regions such as Southeast Asia and Northern Australia. In recent years, there have been reports of an emergence of human melioidosis in other areas, including New Caledonia. Results During standard laboratory analysis in New Caledonia in 2021, a strain of B. pseudomallei was isolated from a goat. The strain was characterized using both MLST and WGS techniques and was found to cluster with previously described local human strains from the area. In parallel, several serological tests (CFT, ELISA, Luminex (Hcp1, GroEL, BPSS1840), arrays assay and a latex agglutination test) were performed on animals from the farm where the goat originated, and/or from three other neighboring farms. Using two commercial ELISA kits, seropositive animals were found only on the farm where the infected goat originated and tests based on recombinant proteins confirmed the usefulness of the Hcp1 protein for the diagnosis of melioidosis in animals. Conclusions Despite the regular reports of human cases, this is the first confirmed case of melioidosis in an animal in New Caledonia. These results confirm the presence of the bacterium in the region and highlight the importance of vigilance for both animal and human health. It is critical that all health partners, including breeders, veterinarians, and biologists, work together to monitor and prevent the spread of the disease.

Published
2024
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2. Le « Goût de la Justice » : la politique artistique de la Chancellerie (1800-1962)

Author

Thomas Deshayes

Subjects
iconography, artists, art and architecture, public commission, chancellery, artistic policy, Fine Arts
Abstract

This article interrogates the existence of a specific artistic policy to the Ministry of Justice. The circuit of ordering or attributing works involves many actors: Fine Arts administration, architects, Keepers of the Seals. This diversity is not without problems in this policy translation. Despite the undeniable aesthetic successes, the difficulty of defining, and preserving throughout time, an iconography of Justice particular to the Chancellery action is problematic. Through works still seen at Place Vendôme and those now preserved elsewhere, the question of Justice’s taste is raised.

Published
2022
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3. La Restauration des sièges du Salon rouge de l’hôtel de la Chancellerie entre usages contemporains et patrimoine

Author

Amandine Cambet, Thomas Deshayes, Carolina Hall, and Antoine Meissonnier

Subjects
furniture, wood, textile, hôtel de Bourvallais, Ministry of Justice, ministerial mansion, Fine Arts
Abstract

In the Bourvallais hotel, headquarters of the French Ministry of Justice for more than 300 years, there are several pieces of furniture classified as “historic monuments”. Among them, there is a remarkable set of 33 mahogany seats with bronze, probably dating from the Bourbon Restoration, characterized by their protomes with lion heads and clawed feet. After years of preparation and waiting, these seats are now undergoing a complete restoration programme.At the end of this study, lacks still remain on the history of the furniture with lion heads from the Ministry of Justice: the conditions of order and arrival at the Bourvallais hotel are not certain, nor the identity of the author. But the information collected in the archives attests to the importance of this first-rate ensemble, from the end of the 1820s. It is not just one of several pieces of furniture, but the main ceremonial ensemble from the Chancellery hotel. After a period of relative oblivion under the French Third Republic, the furniture with lion heads finds its initial vocation of prestige representation in a new setting, the Empire living room, which takes over the function of the hotel's formal living room.The fact that the furniture is still in use must be taken into account in the choice of the upholstery: the choice of a crimson silk embroidered with golden patterns was a compromise, but established on historically documented models. Likewise, the padding was carried out according to the old methods. As for the restoration of the woods, it was done according to heritage restoration standards, aiming at the maximum conservation of old materials.

Published
2022
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5. A genetic variant of Burkholderia mallei detected in Kuwait: Consequences for the PCR diagnosis of glanders

Author

Rachid Aaziz, Vanina Guernier-Cambert, Saad J Alanezi, Mamdouh El Shorbagy, Fabien Vorimore, Yousef S N Alazemi, Maha Al Batel, Ulrich Wernery, Thomas Deshayes, Sabine Delannoy, Claire Bertin, Karine Laroucau, Khaled A W Almutawaa, Attia M Sami, and Koshy Varghese

Subjects
040301 veterinary sciences, Real-Time Polymerase Chain Reaction, Burkholderia mallei, 0403 veterinary science, 03 medical and health sciences, Species Specificity, Zoonoses, Genotype, medicine, Animals, Horses, Insertion sequence, Genotyping, 030304 developmental biology, 0303 health sciences, General Veterinary, General Immunology and Microbiology, biology, Burkholderia pseudomallei, Glanders, 04 agricultural and veterinary sciences, General Medicine, medicine.disease, Complement fixation test, biology.organism_classification, Virology, Kuwait, Multilocus sequence typing, Multilocus Sequence Typing
Abstract

Glanders is a contagious zoonotic disease caused by Burkholderia mallei. Following the detection of glanders positive horses using the OIE complement fixation test, the tissues of two horses were analysed by PCR. While PCR systems targeting the Burkholderia pseudomallei complex gave positive signals, the species-specific PCR systems targeting B. mallei (fliP-IS407A) and B. pseudomallei (orf11)-the OIE recommended targets-resulted in negative signals. However, the presence of B. mallei in these tissues was confirmed with a recently described B. mallei-specific real-time PCR system and genotyping with MLST- and SNP-based methods, performed on the most positive tissue, identified a genotype closely related to B. mallei strains recently isolated in the Middle East. This study leads to recommendations regarding the use of PCR systems for the molecular diagnosis of glanders, especially in regions where the circulating B. mallei strains have not yet been fully genetically characterized.

Published
2020
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8. Molecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014-2017)

Author

Marcus V.D. Falcão, Karine Laroucau, Fabien Vorimore, Thomas Deshayes, Vania L.A. Santana, Karla P.C. Silva, Sergio A. do Nascimento, Roberto S. de Castro, Flabio R. Araújo, and Rinaldo A. Mota

Subjects
Microbiology (medical), Infectious Diseases, Whole Genome Sequencing, Glanders, Zoonoses, Genetics, Animals, Horses, Molecular Biology, Microbiology, Burkholderia mallei, Ecology, Evolution, Behavior and Systematics, Brazil
Abstract

Glanders is an infectious zoonosis caused by Burkholderia (B.) mallei that mainly affects equids. The objective of this work was to provide additional knowledge on the diversity of the strains circulating in Brazil. Six Burkholderia mallei isolates obtained during necropsies of glanderous horses between 2014 and 2017 in two different states (Pernambuco and Alagoas) were analyzed by polymerase chain reaction-high-resolution melting (PCR-HRM). While four strains (9902 RSC, BM_campo 1, BM_campo 3 and UFAL2) clustered in the L3B2 branch, which already includes the Brazilian 16-2438_BM#8 strain, two strains (BM_campo 2.1 and BM_campo 2.2) clustered within the L3B3sB3 branch, which mostly includes older isolates, from Europe and the Middle East. Whole genome sequencing of two of these strains (UFAL2 and BM_campo 2.1), belonging to different branches, confirmed the HRM typing results and refined the links between the strains, including the description of the L3B3Sb3Gp1SbGp1 genotype, never reported so far for contemporary strains. These results suggest different glanders introduction events in Brazil, including a potential link with strains of European origin, related to colonization or trade.

Published
2021

9. Une politique du diorama : L’exemple du musée national de la Marine (1943-1971)

Author

Thomas Deshayes

Subjects
Cultural Studies, Visual Arts and Performing Arts, Museology, Conservation
Abstract

Le musee national de la Marine est l’un des plus anciens musees maritimes du monde. Heritier de la collection formee par l’inspecteur des constructions navales Duhamel du Monceau (1700-1782) et leguee en 1748 au roi Louis XV, il s’installe a partir de 1939 dans l’aile « Passy » du palais de Chaillot, apres un siecle passe au sein du palais du Louvre (Ninderlinder, 2017). La renovation complete dans laquelle le musee est engage (2017-2021) permet de porter un nouveau regard sur des collections...

Published
2019

10. Esquisses pédagogiques Paul Klee, 1925. Une recréation

Author

Hélenne Rolland, Dacha Ferras, Alexandre Morin, Alexis Delestre, Sibyl Lucciani, Justine Duhaussay, Maxime Guillossou, Claire Mouillade, Julie Somé, Inès Malbec, Marie-Léa Seguin, Xiulun Liu, Céline Agaesse, Florine Gouget, Zhuohong Zhang, Claire Cherel, Thomas Deshayes, and Virginie Nicollet

Subjects
General Engineering, Energy Engineering and Power Technology
Published
2018
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11. Molecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014-2017)

Author

FALCÃO, M. V. D., LAROUCAU, K., VORIMORE, F., DESHAYES, T., SANTANA, V. L. A., SILVA, K. P. C., NASCIMENTO, S. A. DO, CASTRO, R. S. DE, ARAUJO, F. R., MOTA, R. A., MARCUS V. D. FALCÃO, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil., KARINE LAROUCAU, Unidade de Zoonoses Bacterianas, Agência Francesa de Saúde e Segurança Alimentar, Ambiental e Ocupacional (ANSES), Maisons-Alfort, França., FABIEN VORIMORE, Unidade de Zoonoses Bacterianas, Agência Francesa de Saúde e Segurança Alimentar, Ambiental e Ocupacional (ANSES), Maisons-Alfort, França., THOMAS DESHAYES, Unidade de Zoonoses Bacterianas, Agência Francesa de Saúde e Segurança Alimentar, Ambiental e Ocupacional (ANSES), Maisons-Alfort, França., VANIA L. A. SANTANA, Ministério da Agricultura, Pecuária e Abastecimento, Recife, Pernambuco, Brasil., KARLA P. C. SILVA, Universidade Federal de Alagoas, Departamento de Medicina Veterinária, Maceió, Alagoas, Brasil., SERGIO A. DO NASCIMENTO, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil., ROBERTO S. DE CASTRO, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil., FLABIO RIBEIRO DE ARAUJO, CNPGC, and RINALDO A. MOTA, Universidade Federal Rural de Pernambuco, Departamento de Medicina Veterinária, Recife, Pernambuco, Brasil.

Subjects
Genotyping, Horses, Burkholderia mallei, PCR-HRM, WGS
Abstract

Glanders is an infectious zoonosis caused by Burkholderia (B.) mallei that mainly affects equids. The objective of this work was to provide additional knowledge on the diversity of the strains circulating in Brazil. Six Burkholderia mallei isolates obtained during necropsies of glanderous horses between 2014 and 2017 in two different states (Pernambuco and Alagoas) were analyzed by polymerase chain reaction?high-resolution melting (PCRHRM). While four strains (9902 RSC, BM_campo 1, BM_campo 3 and UFAL2) clustered in the L3B2 branch, which already includes the Brazilian 16-2438_BM#8 strain, two strains (BM_campo 2.1 and BM_campo 2.2) clustered within the L3B3sB3 branch, which mostly includes older isolates, from Europe and the Middle East. Whole genome sequencing of two of these strains (UFAL2 and BM_campo 2.1), belonging to different branches, confirmed the HRM typing results and refined the links between the strains, including the description of the L3B3Sb3Gp1SbGp1 genotype, never reported so far for contemporary strains. These results suggest different glanders introduction events in Brazil, including a potential link with strains of European origin, related to colonization or trade. Made available in DSpace on 2022-03-23T07:08:15Z (GMT). No. of bitstreams: 1 2022-Genoma-Burkholkderia-mallei.pdf: 970863 bytes, checksum: d4cc1ef661f091d8dce8e161074e59bb (MD5) Previous issue date: 2022

Published
2022

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