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6. Agricultural fertilization with poultry manure results in persistent environmental contamination with the pathogen Clostridioides difficile

Author

Frentrup, Martinique, primary, Thiel, Nadine, additional, Junker, Vera, additional, Behrens, Wiebke, additional, Münch, Steffen, additional, Siller, Paul, additional, Kabelitz, Tina, additional, Faust, Matthias, additional, Indra, Alexander, additional, Baumgartner, Stefanie, additional, Schepanski, Kerstin, additional, Amon, Thomas, additional, Roesler, Uwe, additional, Funk, Roger, additional, and Nübel, Ulrich, additional

Published
2021
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7. Agricultural fertilization with poultry manure results in persistent environmental contamination with the pathogenClostridioides difficile

Author

Frentrup, Martinique, primary, Thiel, Nadine, additional, Junker, Vera, additional, Behrens, Wiebke, additional, Münch, Steffen, additional, Siller, Paul, additional, Kabelitz, Tina, additional, Faust, Matthias, additional, Indra, Alexander, additional, Baumgartner, Stefanie, additional, Schepanski, Kerstin, additional, Amon, Thomas, additional, Roesler, Uwe, additional, Funk, Roger, additional, and Nübel, Ulrich, additional

Published
2021
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8. Airborne bacterial emission fluxes from manure‐fertilized agricultural soil

Author

Thiel, Nadine, primary, Münch, Steffen, additional, Behrens, Wiebke, additional, Junker, Vera, additional, Faust, Matthias, additional, Biniasch, Oliver, additional, Kabelitz, Tina, additional, Siller, Paul, additional, Boedeker, Christian, additional, Schumann, Peter, additional, Roesler, Uwe, additional, Amon, Thomas, additional, Schepanski, Kerstin, additional, Funk, Roger, additional, and Nübel, Ulrich, additional

Published
2020
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9. Functional relationship of particulate matter (PM) emissions, animal species, and moisture content during manure application

Author

Kabelitz, Tina, Ammon, Christian, Funk, Roger, Münch, Steffen, Biniasch, Oliver, Nübel, Ulrich, Thiel, Nadine, Rösler, Uwe, Siller, Paul, Amon, Barbara, Aarnink, André J.A., and Amon, Thomas

Subjects
lcsh:GE1-350, Manure management, Microorganism, Pig, Swine, Emissie & Mestverwaarding, Farm Technology, Agriculture, Poultry, Manure, Fine dust, WIAS, Animals, Agrarische Bedrijfstechnologie, Particulate Matter, Emissions & Manure Valorisation, Dry matter content, Fertilizers, lcsh:Environmental sciences
Abstract

Livestock manure is recycled to agricultural land as organic fertilizer. Due to the extensive usage of antibiotics in conventional animal farming, antibiotic-resistant bacteria are highly prevalent in feces and manure. The spread of wind-driven particulate matter (PM) with potentially associated harmful bacteria through manure application may pose a threat to environmental and human health. We studied whether PM was aerosolized during the application of solid and dried livestock manure and the functional relationship between PM release, manure dry matter content (DM), treatment and animal species. In parallel, manure and resulting PM were investigated for the survival of pathogenic and antibiotic-resistant bacterial species. The results showed that from manure with a higher DM smaller particles were generated and more PM was emitted. A positive correlation between manure DM and PM aerosolization rate was observed. There was a species-dependent critical dryness level (poultry: 60% DM, pig: 80% DM) where manure began to release PM into the environment. The maximum PM emission potentials were 1 and 3 kg t−1 of applied poultry and pig manure, respectively. Dried manure and resulting PM contained strongly reduced amounts of investigated pathogenic and antibiotic-resistant microorganisms compared to fresh samples. An optimal manure DM regarding low PM emissions and reduced pathogen viability was defined from our results, which was 55–70% DM for poultry manure and 75–85% DM for pig manure. The novel findings of this study increase our detailed understanding and basic knowledge on manure PM emissions and enable optimization of manure management, aiming a manure DM that reduces PM emissions and pathogenic release into the environment.

Published
2019

10. TCR signalling network organization at the immunological synapses of murine regulatory T cells

Author

van Ham, Marco, primary, Teich, René, additional, Philipsen, Lars, additional, Niemz, Jana, additional, Amsberg, Nicole, additional, Wissing, Josef, additional, Nimtz, Manfred, additional, Gröbe, Lothar, additional, Kliche, Stefanie, additional, Thiel, Nadine, additional, Klawonn, Frank, additional, Hubo, Mario, additional, Jonuleit, Helmut, additional, Reichardt, Peter, additional, Müller, Andreas J., additional, Huehn, Jochen, additional, and Jänsch, Lothar, additional

Published
2017
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11. Impact of short-term storage on the quantity of extended-spectrum beta-lactamase–producing Escherichia coliin broiler litter under practical conditions

Author

Siller, Paul, Daehre, Katrin, Thiel, Nadine, Nübel, Ulrich, and Roesler, Uwe

Abstract

Applying broiler litter containing extended-spectrum beta-lactamase (ESBL)–producing Escherichia coli(E. coli) to arable land poses a potential risk for humans to get colonized by contact with contaminated soil or vegetables. Therefore, an inactivation of these bacteria before land application of litter is crucial. We performed 2 short-term litter storage trials (one in summer and winter, respectively), each covering a time span of 5 D to investigate the effectiveness of this method for inactivation of ESBL-producing E. coliin chicken litter. Surface and deep litter samples were taken from a stacked, ESBL-positive chicken litter heap in triplicates in close sampling intervals at the beginning and daily for the last 3 D of the experiments. Samples were analyzed quantitatively and qualitatively for ESBL-producing E. coli, total E. coli, and enterococci. Selected isolates were further characterized by whole-genome sequencing (WGS). In the depth of the heap ESBL-producing E. coliwere detected quantitatively until 72 h and qualitatively until the end of the trial in winter. In summer detection was possible quantitatively up to 36 h and qualitatively until 72 h. For surface litter samples a qualitative detection of ESBL-producing E. coliwas possible in all samples taken in both trials. In the deep samples a significant decrease in the bacterial counts of over 2 Log10was observed for total E. coliin the winter and for total E. coliand enterococci in the summer. Genetic differences of the isolates analyzed by WGS did not correlate with survival advantage. In conclusion, short-term storage of chicken litter stacked in heaps is a useful tool for the reduction of bacterial counts including ESBL-producing E. coli. However, incomplete inactivation was observed at the surface of the heap and at low ambient temperatures. Therefore, an extension of the storage period in winter as well as turning of the heap to provide aerobic composting conditions should be considered if working and storage capacities are available on the farms.

Published
2020
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12. The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages

Author

Thiel, Nadine, primary, Keyser, Kirsten A., additional, Lemmermann, Niels A. W., additional, Oduro, Jennifer D., additional, Wagner, Karen, additional, Elsner, Carina, additional, Halenius, Anne, additional, Lenac Roviš, Tihana, additional, Brinkmann, Melanie M., additional, Jonjić, Stipan, additional, Cicin-Sain, Luka, additional, and Messerle, Martin, additional

Published
2016
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14. TCR signalling network organization at the immunological synapses of murine regulatory T cells.

Author

Ham, Marco, Teich, René, Philipsen, Lars, Niemz, Jana, Amsberg, Nicole, Wissing, Josef, Nimtz, Manfred, Gröbe, Lothar, Kliche, Stefanie, Thiel, Nadine, Klawonn, Frank, Hubo, Mario, Jonuleit, Helmut, Reichardt, Peter, Müller, Andreas J., Huehn, Jochen, and Jänsch, Lothar

Abstract

Regulatory T (Treg) cells require T-cell receptor (TCR) signalling to exert their immunosuppressive activity, but the precise organization of the TCR signalling network compared to conventional T (Tconv) cells remains elusive. By using accurate mass spectrometry and multi-epitope ligand cartography (MELC) we characterized TCR signalling and recruitment of TCR signalling components to the immunological synapse (IS) in Treg cells and Tconv cells. With the exception of Themis which we detected in lower amounts in Treg cells, other major TCR signalling components were found equally abundant, however, their phosphorylation-status notably discriminates Treg cells from Tconv cells. Overall, this study identified 121 Treg cell-specific phosphorylations. Short-term triggering of T cell subsets via CD3 and CD28 widely harmonized these variations with the exception of eleven TCR signalling components that mainly regulate cytoskeleton dynamics and molecular transport. Accordingly, conjugation with B cells indeed caused variant cellular morphology and revealed a Treg cell-specific recruitment of TCR signalling components such as PKCθ, PLCγ1 and ZAP70 as well as B cell-derived CD86 into the IS. Together, results from this study support the existence of a Treg cell-specific IS and suggest Treg cell-specific cytoskeleton dynamics as a novel determinant for the unique functional properties of Treg cells. [ABSTRACT FROM AUTHOR]

Published
2017
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15. M27 Expressed by Cytomegalovirus Counteracts Effective Type I Interferon Induction of Myeloid Cells but Not of Plasmacytoid Dendritic Cells

Author

Döring, Marius, primary, Lessin, Irina, additional, Frenz, Theresa, additional, Spanier, Julia, additional, Kessler, Annett, additional, Tegtmeyer, Pia, additional, Dağ, Franziska, additional, Thiel, Nadine, additional, Trilling, Mirko, additional, Lienenklaus, Stefan, additional, Weiss, Siegfried, additional, Scheu, Stefanie, additional, Messerle, Martin, additional, Cicin-Sain, Luka, additional, Hengel, Hartmut, additional, and Kalinke, Ulrich, additional

Published
2014
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16. ZERRISSEN - VERSCHMUTZT - ZERKNICKT DIE RESTAURIERUNG UND KONSERVIERUNG DES GESAMTBE-STANDES DES HISTORISCHEN ARCHIVS DER STADT KÖLN NACH DEM EINSTURZ - SACHSTAND UND PERSPEKTIVEN.

Author

Fischer, Ulrich, Thiel, Nadine, and Henningsen, Imke

Abstract

The article discusses the restoration of the Historical Archive in Cologne (HAStK). Thus, it is stated that with the collapse of the Historical Archives of the City on 3 March 2009, almost the entire archive inventory of over 1,200 years of urban, regional and church history to the extent of about 30,000 meters of shelves had been severely affected, thus leading to an immediate necessity to pursue extensive restoration work of the archive building. Because of this, the use of resources from the archives is not available. Furthermore, the order of almost all stocks had been lost due to the collapse of the archive.

Published
2011

17. What Does She Want? : Current Feminist Art from the First Bank Collection

Author

Haworth, Dale K., Sowder, Lynne, Braulick, Nathan, Charlesworth, Sarah, Thiel, Nadine, Barney, Tina, Borgerding, Jeanette, Goldin, Nan, Cypis, Dorit, Ess, Barbara, Plimpton, Jan, Anderson, Mary, Dwyer, Nancy, Bender, Gretchen, Hanna, Cree, Lawler, Louise, Lane, Ellen Ruth, Thiel, Sue L., Vikesland, Angela, Holzer, Jenny, Simpson, Lorna, Scholz, Donna, Horsfield, Kate, Birnbaum, Dara, Kruger, Barbara, Swindle, Patricia, Rosler, Martha, Haworth, Dale K., Sowder, Lynne, Braulick, Nathan, Charlesworth, Sarah, Thiel, Nadine, Barney, Tina, Borgerding, Jeanette, Goldin, Nan, Cypis, Dorit, Ess, Barbara, Plimpton, Jan, Anderson, Mary, Dwyer, Nancy, Bender, Gretchen, Hanna, Cree, Lawler, Louise, Lane, Ellen Ruth, Thiel, Sue L., Vikesland, Angela, Holzer, Jenny, Simpson, Lorna, Scholz, Donna, Horsfield, Kate, Birnbaum, Dara, Kruger, Barbara, Swindle, Patricia, and Rosler, Martha

Abstract

Introductory notes are followed by statements by women artists and employees of the First Bank Corporation which relate to feminism, art and women's status in the workplace.

Published
1989

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