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Carnoy's solution has been widely undertaken as adjunctive therapy for benign odontogenic tumors. Although its outcomes were highly investigated, little information is available regarding its mechanism of action and the role of chloroform in composition. The aim of this study was to characterize Carnoy's solution (with and without chloroform) in order to survey its physicochemical and pharmacological properties. UV/Vis spectroscopy, Raman microspectroscopy, viscosity assessment, pH analysis, and ion (Fe3+) concentration were studied. All solutions were prepared by the same operator and assayed at each 7 days within a 4-week period. All solutions depicted acidic pH whereas the viscosity was increased by the addition of chloroform. Starting in the seventh day after preparation, solutions with chloroform showed iron precipitation when nonfiltered. Carnoy's solution without chloroform was stable during the survey period as evidenced by Raman spectroscopy. In conclusion, Carnoy's solution possesses an acidic characteristic, it is stable when stored at room temperature, and precipitates iron salts when in the presence of chloroform in a nonfiltered solution. The chloroform increases the viscosity of the solution and makes its pH slightly more acidic when compared to the Carnoy's solution without chloroform. Raman spectroscopy favored the evaluation of components' stability in solutions by using the spectra analysis and the established correlations between the peaks in different periods.

The present work aimed to investigate the antioxidant, anti-inflammatory and wound healing potential of ethyl acetate fraction from Bauhinia ungulata L. (FABU) on in vitro and in vivo models. Wound healing assay using human lung adenocarcinoma A549 cell line was employed to evaluate the ability of FABU in modulating cell migration. In addition, a surgical wound model in C57BL/6 mice was used to study the healing potential of FABU incorporated into gel carbomer 940 (Carbopol®). Evaluation of lipid peroxidation, inflammatory and anti-inflammatory mediator gene expression, rate of wound closure, and histological analysis were done. FABU significantly reduced the gap area in in vitro wound healing assay, 24 h after treatment. In the animal model, FABU at 0.5% topically applied once-daily for 5 days to the surgical wounds significantly reduced the lesion area. Moreover, it significantly decreased the levels of lipid peroxidation in the lesions and decreased the relative gene expression levels of IL-1β and TNF-α in the injured region. In conclusion, our study suggests that Bauhinia ungulata can effectively promote the wound healing, probably by regulating the inflammatory environment during the early stages of the process.

Objective The aim of this study was to compare serum anti-phenolic glycolipid-1 IgA, IgG, and IgM levels in leprosy patients and controls. Method Analysis of anti-PGL-1 IgA, IgG, or IgM in serum samples from multibacillary (MB, n=32) and paucibacillary (PB, n=22) leprosy patients, and in non-endemic controls (n=17), using an indirect enzyme-linked immunosorbent assay. Results A strong correlation between serum IgM and IgA isotypes was found (r=.745, P