Search

Introduction: The emergence of multidrug-resistant pathogens, especially carbapenem-resistant Klebsiella pneumoniae (CRKP), is a pressing public health issue. The limited therapeutic choices further compound this. The escalating resistance to colistin, a crucial last-resort antibiotic, underscores the need for precise and dependable antimicrobial susceptibility testing (AST) methods. Objectives: To evaluate the performance of the Colistin Broth Disk Elution (CBDE) method in comparison to the reference Broth Microdilution (rBMD) method for detecting colistin resistance in CRKP isolates. Methods: A cross-sectional study involving 129 non-duplicate CRKP isolates was conducted at Cho Ray Hospital from October 2023 to December 2023. Colistin susceptibility was assessed using both the BMD and CBDE methods. The agreement between the two methods was measured in terms of categorical agreement (CA) and essential agreement (EA) based on Clinical and Laboratory Standards Institute (CLSI) criteria. Results: Of the 129 CRKP isolates, 23.26 % were identified as colistin-resistant by both BMD and CBDE methods. The CA between the two methods was 100 %, and the EA was 91.47 %, with no major errors (ME) or very major errors (VME) detected. Conclusion: The CBDE method has shown a high level of agreement with the reference BMD method, making it a reliable alternative for the routine detection of colistin resistance in microbiology laboratories. The study's findings strongly support using CBDE as a practical and effective AST method for colistin, thereby contributing to antibiotic stewardship.

Abstract Hypervirulent Klebsiella pneumoniae (hvKp) is a significant cause of severe invasive infections in Vietnam, yet data on its epidemiology, population structure and dynamics are scarce. We screened hvKp isolates from patients with bloodstream infections (BSIs) at a tertiary infectious diseases hospital in Vietnam and healthy individuals, followed by whole genome sequencing and plasmid analysis. Among 700 BSI-causing Kp strains, 100 (14.3%) were hvKp. Thirteen hvKp isolates were identified from 350 rectal swabs of healthy adults; none from 500 rectal swabs of healthy children. The hvKp isolates were genetically diverse, encompassing 17 sequence types (STs), predominantly ST23, ST86 and ST65. Among the 113 hvKp isolates, 14 (12.6%) carried at least one antimicrobial resistance (AMR) gene, largely mediated by IncFII, IncR, and IncA/C plasmids. Notably, the acquisition of AMR conjugative plasmids facilitated horizontal transfer of the non-conjugative virulence plasmid between K. pneumoniae strains. Phylogenetic analysis demonstrated hvKp isolates from BSIs and human carriage clustered together, suggesting a significant role of intestinal carriage in hvKp transmission. Enhanced surveillance is crucial to understand the factors driving intestinal carriage and hvKp transmission dynamics for informing preventive measures. Furthermore, we advocate the clinical use of our molecular assay for diagnosing hvKp infections to guide effective management.

The food and beverage industry in Vietnam has developed dramatically, resulting in a highly competitive market. To win the competition, the restaurant managers not only strive to increase customer satisfaction but also create customers’ positive electronic Word-Of-Mouth (eWOM). This study aims to investigate the relationship between customer satisfaction, customer trust, customer loyalty, and eWOM. The quantitative method was implemented to examine the hypotheses in the research model. Data were collected from Ho Chi Minh City respondents using a convenient sampling method. Self-administrated questionnaires were delivered via the Internet for data collection. After data screening, 299 responses were qualified for analysis. Partial Least Squares Structural Equation Modeling (PLS-SEM) analysis was employed by SmartPLS software version 3.0. The measurement model test showed that one item of customer trust has a factor loading of less than 0.7 as a result, this item was removed from the research model. The structural model evaluation revealed the highly predictive accuracy of the model as all dependent variables have R2 values above 0.5. A bootstrapping test with 5,000 subsamples also showed that customer loyalty significantly influences eWOM, but customer trust does not. Customer satisfaction directly and significantly impacts customer trust and loyalty but has little influence on eWOM. However, the relationship between customer satisfaction and eWOM is significant without the presence of customer loyalty and trust. The indirect effect test also determines the full mediation role of customer loyalty in the relationship between customer satisfaction and eWOM.

Background: The COVID-19 pandemic has affected people of all ages and backgrounds, but pregnant women are a particularly vulnerable group. They are at increased risk of severe illness and death from COVID-19, yet there is limited and conflicting data about the factors that contribute to mortality in this population. In this study, we sought to use machine learning (ML) to predict the possible factors associated with mortality in pregnant women with COVID-19. Methods: We collected data from a large cohort of pregnant women who had been diagnosed with COVID-19 at Hung Vuong Hospital. We included a range of demographic, clinical, and laboratory variables in our analysis, such as age, gestational age, comorbidities, symptoms, and laboratory test results. Our goal was to identify the critical factors that could be used to predict mortality in this vulnerable population. We applied several ML models and analyzed the results to determine the most useful predictors. Results: We analyzed a cohort of 550 pregnant women diagnosed with COVID-19, comprising 525 survivors and 25 deaths. Using ML algorithms, we identified key clinical and patient factors that correlated with mortality risk, such as infection severity, pulse rate, breathing patterns, SpO2 levels, rapid diagnostic tests, internal pathology, breathing difficulties, and changes in consciousness. Additionally, several medical test factors were found to be significant, including white blood cells, neutrophils, platelets, C-reactive protein (CRP), procalcitonin, and chest X-ray abnormalities. Our analysis revealed that the Gaussian Naive Bayes (NB) model was the most accurate, with an AUC score of 0.79, demonstrating its potential as a valuable tool for identifying high-risk patients. Conclusions: Our results suggest that the available data can capture the relationship between COVID-19 symptoms and mortality in pregnant women. More studies are needed to improve the accuracy and generalizability of models for predicting mortality risk in this population.

Xylanases, key enzymes for hydrolyzing xylan, have diverse industrial applications. The bioprocessing of agricultural byproducts to produce xylanase through fermentation approaches is gaining importance due to its significant potential to reduce enzyme production costs. In this work, the productivity of Streptomyces thermocarboxydus TKU045 xylanase was enhanced through liquid fermentation employing wheat bran as the sole carbon source. The maximum xylanase activity (25.314 ± 1.635 U/mL) was obtained using the following optima factors: 2% (w/v) wheat bran, 1.4% (w/v) KNO3, an initial pH of 9.8, an incubation temperature of 37.3 °C, and an incubation time of 2.2 days. Xylanase (Xyn_TKU045) of 43 kDa molecular weight was isolated from the culture supernatant and was biochemically characterized. Analysis through liquid chromatography with tandem mass spectrometry revealed a maximum amino acid identity of 19% with an endo-1,4-β-xylanase produced by Streptomyces lividans. Xyn_TKU045 exhibited optimal activity at pH 6, with remarkable stability within the pH range of 6.0 to 8.0. The enzyme demonstrated maximum efficiency at 60 °C and considerable stability at ≤70 °C. Mg2+, Mn2+, Ba2+, Ca2+, 2-mercaptoethanol, Tween 20, Tween 40, and Triton X-100 positively influenced Xyn_TKU045, while Zn2+, Fe2+, Fe3+, Cu2+, and sodium dodecyl sulfate exhibited adverse impact. The kinetic properties of Xyn_TKU045 were a Km of 0.628 mg/mL, a kcat of 75.075 s−1 and a kcat/Km of 119.617 mL mg−1s−1. Finally, Xyn_TKU045 could effectively catalyze birchwood xylan into xylotriose and xylobiose as the major products.

We present the current state of the art in micro-transfer printing for heterogeneously integrated silicon photonic integrated circuits. The versatility of the technology is highlighted, as is the way ahead to make this technology a key enabler for next-generation photonic systems-on-chip.

Objectives: In 1990, Ho Chi Minh City started Community water fluoridation (CWF) at 0.7 ppm F, and in 2000, it was adjusted to 0.5 ppm F. Here, we analyzed dental caries and fluorosis data in Ho Chi Minh City to explore commonalities associated with CWF among 12-year-old children. Methods: Dental caries and fluorosis data were collected in 1989, 2003, 2012, and 2019 (N = 4773). Trained dentists scored dental caries using the WHO detection criteria and fluorosis using Dean's Fluorosis Index. We used these data and the k-prototypes method by the R package to identify clusters of participants with shared clinical and water fluoride levels. Results: We used datasets 1 (4773 participants) and 2 (4194 participants, missing fluorosis data in 1989). K-prototypes analysis identified three clusters in each dataset. Cluster 1, with 80 % of the sample at 0.5 ppm F area characterized by low caries and fluorosis scores. Cluster 2 with 60 % of the sample non-fluoridated area had high caries and low fluorosis scores. Cluster 3, with 75 % of the sample in 0.7 ppm area, had low caries but borderline high fluorosis scores. Conclusion: Identifying three clusters based on clinical and environmental scores supports the decision to fluoridate the water to prevent caries (0–0.7 ppm) and the shift from 0.7 to 0.5 ppm to keep the caries preventive effect while reducing the risk of fluorosis. Clinical significance: Our results support the effectiveness of CWF in preventing dental caries and the appropriateness of changing the F concentration to reduce the risk of fluorosis while maintaining its effectiveness.

Understanding the relationship between fish parental age and offspring performance over their life cycle is crucial for aquaculturists. This study investigates the effect of parental age for both 1- and 2-year-old fish on the growth and survival rates of their larval to grow-out stages for two wild and domesticated strains of bighead catfish (Clarias macrocephalus). Fish were sampled from the Vietnamese Mekong Delta where wild adult fish were collected from a conservation area in Ca Mau Province and domesticated fish were taken from a hatchery in Can Tho. Four offspring treatments of 1- and 2-year-old broodstock strains were reared for 3 successive periods: 40 days for fingerling rearing in static tanks with water exchange, 60 days for juveniles, and 90 days for grow-out in recirculating systems. The final weight of fish at 190 days varied from 96.7 to 144.7 g. The growth of offspring was not affected by parental age (P > 0.05), over the 3 rearing periods. However, the domesticated fish strain showed significantly better growth rates than the wild fish strain (P

Root biomass and distribution are influenced by abiotic factors, such as topography and soil physicochemical properties, determining belowground productivity. Hence, we investigated the variation in root biomass and vertical root distribution based on the topography, soil physicochemical properties, and tree influence index, and their relationships, across soil depths (0–10 cm, 10–20 cm, and 20–30 cm) and topographical gradients in a warm-temperate forest in Mt. Duryun, Republic of Korea. Two contrasting research sites were established: a lower slope oriented at ≤3° and an upper slope with a slope of 30°. Each site comprised eleven 400 m2 sampling plots from which root samples from various diameter classes (10 mm) were collected. While the bulk density increased with soil depth in the lower slope, the organic matter, available phosphorus, Ca2+, and Mg2+ showed a reversed pattern. Linear mixed-effects models generally revealed significant negative correlations between root biomass and soil pH, total nitrogen, and cation exchange capacity, particularly in small roots (βstd = −1.03 to −1.51) and coarse roots (βstd = −6.30). Root biomass exhibited a 10–15% increase in the upper slope compared to the lower slope, particularly in fine (median = 52.0 g m2–65.64 g m2) and medium roots (median = 56.04 g m2–69.52 g m2) at a 0–20 cm soil depth. While no significant correlation between root biomass and the tree influence index was found on the lower slope, a different pattern was found on the upper slope. Our results indicate that the variation in root biomass and distribution can also be explained by the differences in the soil environment and topographical positions.

We investigate the existence and stability of mild solutions of Boussinesq system in the weak-Lorentz spaces with Muckenhoupt weight in the framework of half-spaces. Our strategy starts with the corresponding linear system, for which we combine the weighted $ L^p-L^q $ Lp−Lq-smoothing estimates and interpolation functors to prove Yamazaki-type inequality and establish the existence of the bounded mild solutions on the half-line $ \mathbb {R}_+ $ R+. Then we use the results of linear systems and fixed point arguments to establish the existence, uniqueness and stability of the mild solutions to the Boussinesq systems. [ABSTRACT FROM AUTHOR]

Urbanization and associated forest conversions have given rise to a continuum of native (forest fragments) and modified (artificial grasslands and perennial ecosystems) land-use types. However, little is known about how these shifts affect soil and fine-root compartments that are critical to a functioning carbon and nutrient circulation system. In this study, soil physicochemical properties, fine-root mass, and vertical distribution patterns were investigated in four representative urban land-use types: grassland (ZJ), perennial agroecosystem (MP), broadleaf deciduous forest patch (QA), and coniferous evergreen forest patch (PD). We quantified the fine-root mass in the upper 30 cm vertical profile (0–30 cm) and at every 5 cm depth across three diameter classes (−2) > PD (487.2 g m−2) > ZJ (440.1 g m−2) > MP (98.3 g m−2). The fine-root mass of ZJ and MP was correlated with soil nutrients, which was attributed to intensive management operations, while the fine-root mass of QA and PD had a significant relationship with soil organic matter due to the high inputs from forest litter. Very fine roots (

Abstract Introduction The emergence of widespread amphetamine-type stimulants (ATSs) usage has created significant challenges for drug control and treatment policies in Southeast Asian countries. This study analyses the development of drug policies and examines current treatment program constraints in Vietnam to deal with ATS misuse. The aim was to gain insights that may be useful for national and international drug-related policy development and revision. Methods A desk review of national policy documents and 22 in-depth key informant interviews were conducted from 2019 to 2021. Thematic content analysis was employed to identify key themes and their connections. Results Analysis identified Vietnam’s 30-year history of developing policies and formulating strategies to reduce supply, demand, and harm from illicit drugs. With the increasing number of people who use ATS (PWUA), Vietnam has recently promoted harsh policy and law enforcement to deter drug use and supply. This policy trend prevails in many Asian countries. The three main constraints in dealing with ATS misuse emerged from punitive and restrictive drug policies. First, the general public believed that Centre-based compulsory treatment (CCT) is the only appropriate treatment for all types of illicit drug addiction despite its low-quality service provision. The rigid drug policy has led to social persuasion with impractical expectations for CCT effectiveness. Second, the emphasis on punishment and detention has hampered new drug treatment service development in Vietnam. CCT has become monopolistic in the context of impoverished services. Third, people who use drugs tend to hide their needs and avoid formal treatment and support services, resulting in declined social coherence. Conclusion While new drugs are constantly evolving, the current law enforcement approach potentially constrains expertise to adopt effective treatment services. This study suggests that the top-down policing mechanism presently hinders the development of an appropriate intervention strategy for ATS misuse and diminishes social support to service providers.

Purple sweet potato has been gradually increasing in terms of planting and consumption but, after harvesting, the leaves of the sweet potato are often discarded as waste or used for animal feed. The aim of this study was to determine the effect of drying methods on the quality of sweet potato leaf powder and its storage capacity. The nutritional and antioxidant properties of powder were analyzed. The results showed that hot air-drying could preserve the antioxidant properties more than the sun-drying method. Both methods could produce a product with high nutritional value, especially a high amount of protein and fiber. This could be considered as a supplementation ingredient for the food industry. Moreover, after storage at room temperature for 12 weeks, a slight increase in moisture content and decrease in antioxidant properties were found. Further, the application of this ingredient should be further investigated in food application to improve the income for local farmers as well as reduce the environmentally harmful waste.

OBJECTIVES Improved life expectancy has increased the prevalence of older adults living with multimorbidity which likely deteriorates their health-related quality of life (HRQoL). However, relatively little is known about patterns and the relationships of multimorbidity by HRQoL status in older adults. METHODS Individuals aged 65 or older from the Korea National Health and Nutrition Examination Survey V-VII (2010-2018) were analyzed. HRQoL was assessed by the EuroQoL-5 dimensions questionnaire and categorized as poor, normal, or good. The impact of multimorbidity on HRQoL was evaluated using logistic regression. The patterns and inter-relationships between multimorbidity, stratified by HRQoL groups, were analyzed using the association rules and network analysis approach. RESULTS Multimorbidity was significantly associated with poor HRQoL (3 or more diseases vs. none; adjusted odds ratio, 2.70; 95% confidence interval, 2.10 to 3.46). Hypertension, arthritis, hyperlipidemia, and diabetes were the most prevalent diseases across all HRQoL groups. Complex interrelationships of morbidities, higher prevalence, and node strengths in all diseases were observed in the poor HRQoL group, particularly for arthritis, depression, and stroke, compared to other groups (1.5-3.0 times higher, p

In this work, we study the existence, uniqueness and exponential stability of periodic mild solution of the Boussinesq system on the real hyperbolic manifold $ \mathbb {H}^d(\mathbb {R}) $ Hd(R) ( $ d \geqslant 2 $ d⩾2). We consider a couple of Ebin-Marsden's Laplace and Laplace–Beltrami operators associated with the corresponding linear system. Our method is based on the dispersive and smoothing estimates of semigroups generated by Ebin-Marsden's Laplace and Laplace–Beltrami operators. First, we prove the existence and uniqueness of the bounded periodic mild solution for the linear system. Next, using the fixed point arguments, we pass from the linear system framework to the semilinear system framework to establish the existence of the periodic mild solution. Additionally, we prove the unconditional uniqueness of periodic mild solution for the Boussinesq system on hyperbolic manifolds. Finally, we establish the exponential stability of periodic mild solution. [ABSTRACT FROM AUTHOR]

Abstract There are growing concerns on stress among nurses in low- and middle-income countries (LMICs) in South-East Asia. It is important to improve mental health among nurses in these countries. The objective of this study was to examine the efficacy of two types of newly developed smartphone-based stress management programs in improving depressive and anxiety symptoms among hospital nurses in Vietnam. This study was a three-arm (including two intervention groups and one control group) randomized trial. Participants were recruited from nurses in a large general hospital in Hanoi, Vietnam. Two types (free-choice and fixed sequential order) of smartphone-based stress management programs were developed. Participants were randomly allocated to Program A (a free-choice, multimodule stress management), Program B (a fixed-order, internet cognitive behavioral therapy, iCBT), or a control group (treatment as usual). The depressive and anxiety symptoms were measured by using the Depression Anxiety and Stress Scales at baseline, 3-, and 7-month follow-up surveys. 951 participants were randomly allocated to each of the three groups. Program B showed a statistically significant effect on improving depressive symptoms at 3-month (p = 0.048), but not at 7-month (p = 0.92); Cohen’s d was − 0.18 (95% CI − 0.34 to − 0.02) and 0.03 (95% CI − 1.00 to 1.05), respectively. Program A failed to show a significant intervention effect on any of the outcomes at 3- or 7-month follow-up (p > 0.05). Despite the small effect size, the present fixed-order iCBT program seems effective in improving depression of hospital nurses in Vietnam. A public health impact of the intervention can be scalable, when considering its accessibility and minimal cost. Trial registration number: The study protocol is registered at the UMIN Clinical Trials Registry (UMINCTR; ID = UMIN000033139). Registered date of the protocol is 1st Jul. 2018. https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000037796

An Giang is one of the provinces in the Mekong delta that is greatly affected by flood events, which brings damage and devastation to life and property. This study practices the application of Sentinel-1A images to monitor the distribution of flood depths in the An Giang province in 2019 as well as applies regression correlation and thresholding to scattering value analysis. The research results indicated the exponential regression model on the VV polarization images had correlation coefficients (r) in August, September, and October ranging from 0.8398 to 0.9764 and determination coefficients (R2) ranging from 0.7896 to 0.9533. Results from the map of current flood depth showed that the flood depth ranged from 0-250 cm, which corresponded to four flood levels. The flood area increased from August to October with the largest flooded area being 89,606.82 ha (accounting for 26.15%) mainly on rice lands and in eight urban districts including An Phu, Tinh Bien, Chau Thanh, Chau Phu, Phu Tan, Tri Ton, Chau Doc, and Long Xuyen city. The limit of flood depth determined by using the Sentinel-1A images was below 145 cm. Above this value, the scattering in the image is not significantly different from the actual submerged depth.

ABSTRACTGram-negative marine bacterium Vibrio parahaemolyticus is an important aquatic pathogen and has been demonstrated to be the causative agent of acute hepatopancreatic necrotic disease (AHPND) in shrimp aquaculture. The AHPND-causing V. parahaemolyticus strains contain a pVA1 plasmid encoding the binary PirAVP and PirBVP toxins, are the primary virulence factor that mediates AHPND and mortality in shrimp. Since PirABVP toxins are secreted extracellularly, one can hypothesize that PirABVP toxins would aggravate vibriosis in the aquatic environment. To address this, in vivo and in vitro experiments were conducted. Germ-free Artemia franciscana were co-challenged with PirABVP toxins and 10 Vibrio spp. The in vivo results showed that PirABVP toxin interact synergistically with MM30 (a quorum sensing AI-2 deficient mutant) and V. alginolyticus AQ13-91, aggravating vibriosis. However, co-challenge by PirABVP toxins and V. campbellii LMG21363, V. parahaemolyticus CAIM170, V. proteolyticus LMG10942, and V. anguillarum NB10 worked antagonistically, increasing the survival of Artemia larvae. The in vitro results showed that the addition of PirABVP toxins significantly modulated the production of the virulence factors of studied Vibrio spp. Yet these in vitro results did not help to explain the in vivo results. Hence it appears that PirABVP toxins can aggravate vibriosis. However, the dynamics of interaction is strain dependent.

Under globalisation, comparative educational studies have become more relevant. This paper is the first of its kind to compare education and training for principals in Vietnam and the U.S. and will offer among the few systematic studies on school leadership in Vietnam. Utilizing previous research on educational leadership and culture in the U.S. and Vietnam, and survey results from Teaching and Learning International Survey (TALIS) from the Organization for Economic Cooperation and Development (OECD) in 2018, I used t-test analyses to test several hypotheses about the differences between principals in the two countries. The findings suggest differences in age, gender, managerial experience, teaching obligation, and obtained levels of education and training between American principals and Vietnamese principals. Principals in the U.S. are younger and less experienced than principals in Vietnam. Level of education and need for professional development programs potentially indicate that American principals receive more thorough training in their education compared to Vietnamese principals. Accordingly, this comparative study provides valuable insights for both countries and contributes to diversifying the literature on educational leadership which the West has long dominated.

Introduction: Amphetamine-type stimulants (ATS) use is a global concern due to increased usage and the harm to physical, mental, and social well-being. The objective of this overview of systematic reviews is to summarise trial results of psychosocial interventions and describe their efficacy and safety.Methods: We searched seven bibliographic databases to November 2020 for systematic reviews examining ATS misuse treatment by psychosocial interventions. Given the apparent incompleteness of the included reviews, we undertook a supplemental meta-analysis of all eligible primary studies.Results: We included 11 systematic reviews of moderate to high quality and 39 primary studies which assessed the outcomes of psychosocial interventions on people who use ATS. The key findings include: (1) There were conflicting results about the effectiveness of psychosocial interventions among reviews, which may confuse decision-makers in selecting treatment. (2) In the supplemental meta-analysis, relative to usual care (only counselling or self-help materials), membership of a psychological intervention group was associated with an important reduction in drug usage [risk ratio (RR) 0.80, 95% CI: 0.75 to 0.85]. Patients in psychological interventions used injectables substantially less [odds ratio (OR) 0.35, 95% CI: 0.24 to 0.49]. The risk of unsafe sex in the psychosocial intervention group was lower than in the control group (RR 0.49, 95% CI: 0.34 to 0.71). The combination of therapies reduced 1.51 day using drugs in the preceding 30 days (95% CI: −2.36 to −0.67) compared to cognitive behavioural therapy intervention alone. (3) Compared to usual care, cognitive behavioural therapy was less likely to be retained at follow-up (RR 0.89, 95% CI: 0.82 to 0.97; high-quality evidence). However, the additional of contingency management strategy can make an important improvement upon retention (RR 1.42, 95%CI: 1.25 to 1.62).Authors' Conclusions: Integrated models are more effective than a single-treatment strategy. Comprehensive and sustained psychosocial interventions can help to reduce use of ATS and other drugs, risk behaviours and mental disorders, and significantly improve treatment adherence.

Proteinaceous wastes from the fishery process are an abundant renewable resource for the recovery of a variety of high-value products. This work attempted to utilize several proteinaceous wastes to produce proteases using the Streptomyces speibonae TKU048 strain. Among different possible carbon and nitrogen sources, the protease productive activity of S. speibonae TKU048 was optimal on 1% tuna head powder. Further, the casein/gelatin/tuna head powder zymography of the crude enzyme revealed the presence of three/nine/six proteases, respectively. The crude-enzyme cocktail of S. speibonae TKU048 exhibited the best proteolytic activity at 70 °C and pH = 5.8. Sodium dodecyl sulfate strongly enhanced the proteolytic activity of the cocktail, whereas FeCl3, CuSO4, and ethylenediaminetetraacetic acid could completely inhibit the enzyme activity. Additionally, the crude-enzyme cocktail of S. speibonae TKU048 could efficiently enhance the 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities of all tested proteinaceous materials including the head, viscera, and meat of tuna fish; the head, viscera, and meat of tilapia fish; the head, meat, and shell of shrimp; squid pen; crab shell; and soybean. Taken together, S. speibonae TKU048 revealed potential in the reclamation of proteinaceous wastes for protease production and antioxidant preparation.

This article discussed Vietnam’s ongoing efforts to decentralize the health system and its fitness to respond to global health crises as presented through the Covid-19 pandemic. We used a general review and expert’s perspective to explore the topic. We found that the healthcare system in Vietnam continued to decentralize from a pyramid to a wheel model. This system shifts away from a stratified technical hierarchy of higher- and lower-level health units (pyramid model) to a system in which quality healthcare is equally expected among all health units (wheel model). This decentralization has delivered more quality healthcare facilities, greater freedom for patients to choose services at any level, a more competitive environment among hospitals to improve quality, and reductions in excess capacity burden at higher levels. It has also enabled the transformation from a patient-based traditional healthcare model into a patient-centered care system. However, this decentralization takes time and requires long-term political, financial commitment, and a working partnership among key stakeholders. This perspective provides Vietnam’s experience of the decentralization of the healthcare system that may be considered as a useful example for other countries to strategically think of and to shape their future system within their own socio-political context.

ObjectiveFamilial transmission can possibly influence the infection and treatment of Helicobacter pylori. This study aimed to describe the prevalence of H. pylori infection and outcomes of eradication treatment among Vietnamese patients who live in the same households.MethodsWe conducted a prospective cohort study of Vietnamese household members with upper gastrointestinal complaints. Participants received esophagogastroduodenoscopy and H. pylori testing. The H. pylori-positive patients were treated and asked to return for follow-up within 4 months. To explore factors associated with H. pylori infection at baseline, we performed multilevel logistic regression to account for the clustering effect of living in the same households. To explore factors associated with eradication failure, we used Poisson regression with robust variance estimation to estimate the risk ratio.ResultsThe prevalence of H. pylori infection was 83.5% and highest among children ConclusionH. pylori infection was common among people living in the same households. Eradication success for H. pylori was higher for the tetracycline sequential regimen. More research should be focused on how family factors influence H. pylori infection and on eradication treatment.

Background and Aims. Age cutoff is an important factor in deciding whether esophagogastroduodenoscopy (EGD) is necessary for patients presenting with upper gastrointestinal symptoms. However, the cutoff value is significantly different across populations. We aimed to determine the age cutoff for EGD that assures a low rate of missing upper gastrointestinal malignancy (UGIM) and to assess the yield of prompt EGD in Vietnamese patients presenting with upper gastrointestinal symptoms. Methods. All EGDs performed in outpatients during a 6-year period (2014–2019) at a tertiary hospital that provided an open-access endoscopy service were retrospectively reviewed. Repeat or surveillance EGDs were excluded. Different age cutoffs were evaluated in terms of their prediction of the absence of UGIM. The yield of endoscopy to detect one malignancy (YoE) was also calculated. Results. Of 472,744 outpatients presenting with upper gastrointestinal symptoms, there were 2198 (0.4%) patients with UGIM. The median age and male-to-female ratio of patients with UGIMs were 57.9 ± 12.5 years and 2.5 : 1, respectively. The YoEs in patients 60 years of age were 10 per 1000 EGDs, respectively. The age cutoffs of 30 years in females and 35 years in males could detect 98.2% (95% CI: 97.7%–98.8%) of UGIM cases with a YoE of about 1 per 1000 EGDs. Conclusions. The age cutoff for EGD in Vietnamese should be lower than that recommended by current international guidelines. The strategy of prompt EGD showed a low YoE, and its cost-effectiveness requires further investigation.

OBJECTIVES The prevalence of diabetes mellitus is rapidly increasing in Vietnam, particularly among adults aged over 45 years. This study estimated trends in diabetes and prediabetes prevalence and determined risk factors in Vietnamese adults (over 45 years). METHODS A cross-sectional study was conducted based on data from an annual diabetes screening program among people aged 45-69 years in an urban city in central Vietnam (Da Nang). Joinpoint regression analyses were performed to calculate the annual percentage change and ptrend-values. Multinomial logistic regression analysis was used to determine risk factors. RESULTS In total, 3,765 men and 9,149 women were included in this analysis. The age-adjusted prevalence of diabetes and prediabetes in 2017 was 11.4% and 52.9%, respectively. The prevalence of diabetes was higher in men (15.1%) than in women (10.3%), but that of prediabetes was similar in both genders (53.4% vs. 52.8%). The prevalence of prediabetes significantly increased during the study period, whereas no upward or downward trend for diabetes was observed. The prevalence of obesity, abdominal obesity, hypertension, and dyslipidemia showed no obvious trend. Obesity, a high waist-to-hip ratio, hypertension, more severe abdominal obesity, and dyslipidemia were significantly associated with a higher risk of diabetes and prediabetes. CONCLUSIONS Diabetes and prediabetes were more prevalent among people aged over 45 years than in the general population. Da Nang has experienced a marked increase in the prevalence of prediabetes. These findings have significant implications regarding the need for nationwide public health interventions and management aiming at diabetes prevention and control.

Mangrove forests have been globally recognised as their vital functions in preventing coastal erosion, mitigating effects of wave actions and protecting coastal habitats and adjacent shoreline land-uses from extreme coastal events. However, these functions are under severe threats due to the rapid growth of population, intensive shrimp farming and the increased intensity of severe storms in Hau Loc and Nga Son districts, Thanh Hoa province. This research was conducted to monitor spatial-temporal changes in mangrove extents using Landsat and Sentinel imageries from 2005 to 2018. Unsupervised and supervised classification methods and vegetation indices were tested to select the most suitable classification method for study sites, then to quantify mangrove extents and their changes in selected years. The findings show that supervised classification was the most suitable in study sites compared to vegetation indices and unsupervised classification. Mangrove forest extents increased by 7.5 %, 38.6 %, and 47.8 % during periods of 2005 - 2010, 2010 - 2015 and 2015 - 2018, respectively. An increase of mangrove extents resulted from national programs of mangrove rehabilitation and restoration during 2005- 2018, increased by 278.0 ha (123.0 %).

Mangrove forests are important and known as one of the most productive ecosystems in the tropics. They reduce the impacts of extreme events, provide important breeding grounds for aquatic species and build the resilience of ecosystem-dependent coastal communities. On the contrary, they are also known as one of the most threatened and vulnerable ecosystems worldwide, which have experienced a dramatic decline due to extensive coastal development during the last half-century. Remote sensing techniques have demonstrated a high potential to detect, identify, map, and monitor mangrove conditions and its changes, which is reflected by a large number of scientific papers published on this topic. The aim of this study was to investigate the multi-decadal changes of mangrove forests selected communes in Hai Phong city, North Vietnam, based on using Landsat and Sentinel 2 data from 2000 to 2018. The study used these continuous steps: 1) data pre-processing; 2) image classification using Normalized Difference Vegetation Index; 3) accuracy assessments; and 4) multi-temporal change detection and spatial analysis of mangrove forests. The classification maps in comparison with the ground reference data showed the satisfactory agreement with the overall accuracy was higher than 80.0%. From 2000 to 2018, the areas of mangrove forests in the study regions increased by 584.2 ha in Dai Hop and Bang La communes (Region 1) and by 124.2 ha in Tan Thanh, Ngoc Xuyen and Ngoc Hai communes (Region 2), mainly due to the boom of mangrove planting projects and good mangrove management at the local community level.

Nghiên cứu nhằm đánh giá ảnh hưởng của các nguồn cá cá trê vàng bố mẹ đến các chỉ tiêu sinh sản và sự phát triển của cá bột. Ba nguồn cá bố mẹ gồm 2 nguồn cá tự nhiên ở Cà Mau (CM) và Long An (LA) và nguồn 1 nuôi ở Cần Thơ (CT) được nuôi vỗ ba tháng. Khi cá đạt giai đoạn thành thục, 6 đến 9 cặp cá từ mỗi nguồn được lai chéo theo 9 tổ hợp. Kết quả cho thấy cá cái CM có sức sinh sản thực tế thấp nhất (25.700 trứng/kg) nhưng đường kính trứng lớn nhất (1,77 mm), khác biệt có ý nghĩa (P

Fishery by-products (FBPs) have been increasingly investigated for the extraction and production of a vast array of active molecules. The aim of this study was to produce phenazine compounds from FBPs via microbial fermentation and assess their novel antinematode effect. Among various FBPs, squid pen powder (SPP) was discovered as the most suitable substrate for phenazine production by Pseudomonas aeruginosa TUN03 fermentation. Various small-scale experiments conducted in flasks for phenazine production indicated that the most suitable was the newly designed liquid medium which included 1% SPP, 0.05% MgSO4, and 0.1% Ca3(PO4)2 (initial pH 7). Phenazines were further studied for scale-up bioproduction in a 14 L bioreactor system resulting in a high yield (22.73 µg/mL) in a much shorter cultivation time (12 h). In the fermented culture broth, hemi-pyocyanin (HPC) was detected as a major phenazine compound with an area percentage of 11.28% in the crude sample. In the bioactivity tests, crude phenazines and HPC demonstrate novel potential nematicidal activity against black pepper nematodes, inhibiting both juveniles (J2) nematodes and egg hatching. The results of this work suggest a novel use of SPP for cost-effective bioproduction of HPC, a novel potential nematodes inhibitor. Moreover, the combination of MgSO4 and Ca3(PO4)2 was also found to be a novel salt composition that significantly enhanced phenazine yield by P. aeruginosa fermentation in this work.

α-Amylase inhibitors (aAIs) have been applied for the efficient management of type 2 diabetes. The aim of this study was to search for potential aAIs produced by microbial fermentation. Among various bacterial strains, Pseudomonas aeruginosa TUN03 was found to be a potential aAI-producing strain, and shrimp heads powder (SHP) was screened as the most suitable C/N source for fermentation. P. aeruginosa TUN03 exhibited the highest aAIs productivity (3100 U/mL) in the medium containing 1.5% SHP with an initial pH of 7–7.5, and fermentation was performed at 27.5 °C for two days. Further, aAI compounds were investigated for scaled-up production in a 14 L-bioreactor system. The results revealed a high yield (4200 U/mL) in a much shorter fermentation time (12 h) compared to fermentation in flasks. Bioactivity-guided purification resulted in the isolation of one major target compound, identified as hemi-pyocyanin (HPC) via gas chromatography-mass spectrometry and nuclear magnetic resonance. Its purity was analyzed by high-performance liquid chromatography. HPC demonstrated potent α-amylase inhibitory activity comparable to that of acarbose, a commercial antidiabetic drug. Notably, HPC was determined as a new aAI. The docking study indicated that HPC inhibits α-amylase by binding to amino acid Arg421 at the biding site on enzyme α-amylase with good binding energy (−9.3 kcal/mol) and creating two linkages of H-acceptors.

In this paper, we measure volatility spillovers among eleven stock markets, including five developed markets (the United States, Japan, Germany, the United Kingdom, Hong Kong) and six Southeast Asian developing markets (Indonesia, Malaysia, Philippines, Singapore, Thailand and Vietnam) over the 25-year period from January 1, 1993 to December 31, 2017. Employing the GARCH-DCC model and non-parametric sign tests on the correlations between developed markets and emerging markets, we find that correlations between developed markets and the Southeast Asian markets have risen sharply during periods of crisis, indicating the existence of volatility spillover effects from the developed markets to emerging ones. Full sample analysis suggests that volatility spillover from Japanese and the UK markets to the Southeast Asian emerging markets is stronger and more apparent than those transmitted from the US and Germany markets. Sub-sample analysis is able to identify the markets transmitting shocks to others. Results also suggest that Vietnam market is not fully integrated to the regional and global markets.

This study aimed to reuse groundnut oil processing by-product, groundnut cake (GNC) for the low-cost production of prodigiosin (PG) via microbial technology and to assess its novel potential application for the management of black pepper nematodes. Serratia marcescens TUN02 was found as the most active PG-producing strain. Various small-scale experiments conducted in flasks indicated that GNC at 1% may be used as the sole carbon/nitrogen source for cost-effective PG production by fermentation. Notably, no further commercial ingredients and salts are required to supplement into the culture medium of this fermentation. PG was further investigated for scale-up production in a 14-L bioreactor system and PG was produced at high yield (6886 mg/L) with large-scale volume (4 L) in a short cultivation time (10 h). PG was then purified and its nematicidal activity was evaluated and showed effective inhibition of juveniles and egg hatching of Meloidogyne incognita species, harmful on black pepper, with low IC50 values of 0.2 and 0.32 mg/mL, respectively. The simple medium containing 1% GNC is the first report of cost-effective biosynthesis of PG, as well as potential in vitro anti-egg hatching activity of PG. These results indicated the potential application of GNC for low-cost bioproduction of PG for promising and novel use in the management of black pepper nematodes.

Cone-beam computed tomography (CBCT) is widely used in dental imaging, small animal imaging, radiotherapy, and non-destructive industrial inspection. The quality of CBCT images depends on the precise knowledge of the CBCT system's alignment. We introduce a distinct procedure, 'precision alignment loop (PAL)', to calibrate any CBCT system with a circular trajectory. We describe the calibration procedure by using a line-beads phantom, and how PAL determines the misalignments from a CBCT system. PAL also yields the uncertainties in the simulated calibration to give an estimate of the errors in the misalignments. From the analytical simulations, PAL can precisely obtain the source-to-rotation axis distance, and the geometric center G, 'the point in z -axis meets the detector', where the z -axis is coincident with the line from the x-ray source that intersects the axis of the rotation orthogonally. The uncertainties of three misalignment angles of the detector are within ±0.05°, which is close to ±0.04° for the results of Yang et al (2017 Med. Phys. 44 1692), but our method is easy and simple to implement. Our distinct procedure, on the other hand, yields the calibration of a micro-CT system and an example of reconstructed images, showing our calibration method for the CBCT system to be simple, precise, and accurate. [ABSTRACT FROM AUTHOR]