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Your search keyword '"Theijsmeijer, A P"' showing total 8 results
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8 results on '"Theijsmeijer, A P"'

2. Global Ground-Based Electro-Optical and Radar Observations of the 1999 Leonid Shower: First Results

Author

Brown, P., Campbell, M.D., Ellis, K.J., Hawkes, R.L., Jones, J., Gural, P., Babcock, D., Barnbaum, C., Bartlett, R.K., Bedard, M., Bedient, J., Beech, M., Brosch, N., Clifton, S., Connors, M., Cooke, B., Goetz, P., Gaines, J. K., Gramer, L., Gray, J., Hildebrand, A.R., Jewell, D., Jones, A., Leake, M., LeBlanc, A.G., Looper, J.K., McIntosh, B.A., Montague, T., Morrow, M.J., Murray, I.S., Nikolova, S., Robichaud, J., Spondor, R., Talarico, J., Theijsmeijer, C., Tilton, B., Treu, M., Vachon, C., Webster, A.R., Weryk, R., and Worden, S.P.

Published
1998
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6. Constitutive expression of E-selectin and vascular cell adhesion molecule-1 on endothelial cells of hematopoietic tissues

Author

Schweitzer, K. M., Dräger, A. M., van der Valk, P., Thijsen, S. F., Zevenbergen, A., Theijsmeijer, A. P., van der Schoot, C. E., Langenhuijsen, M. M., Other departments, and Landsteiner Laboratory

Subjects
Adult, Base Sequence, Hematopoietic System, Molecular Sequence Data, Infant, Newborn, Antibodies, Monoclonal, Vascular Cell Adhesion Molecule-1, Antigens, CD34, Hematopoietic Stem Cells, Polymerase Chain Reaction, Liver, Antibody Specificity, Bone Marrow, embryonic structures, Cell Adhesion, Humans, Endothelium, Vascular, RNA, Messenger, E-Selectin, Cells, Cultured, Spleen, Research Article
Abstract

The first step in the homing of hematopoietic progenitor cells (HPCs) from the peripheral blood to the bone marrow involves an adhesion molecule-dependent contact with human bone marrow endothelial cells (HBMECs). In the present study we describe the constitutive expression of two of these molecules, E-selectin and vascular cell adhesion molecule-1 (VCAM-1), on endothelial cells of hematopoietic tissues. Immunophenotypic analysis of tissue sections of hematopoietically active (human adult and fetal bone marrow, fetal spleen, fetal liver, and adult spleen with extramedullary hematopoiesis) and inactive tissues (human adult spleen, lymph node, appendix, and liver; and fetal lung and fetal intestine) revealed that E-selectin and VCAM-1 are selectively expressed on endothelial cells of adult and fetal hematopoietic organs. These results were validated by means of reverse-transcriptase polymerase chain reaction. Adhesion studies revealed that binding of normal mobilized peripheral blood HPCs to HBMECs was completely inhibited by preincubation of HBMECs with anti-E-selectin (ENA2), whereas no effect of anti-VCAM-1 (1G11B1) was detected. These results suggest that E-selectin plays a role in the homing of HPCs and that its constitutive expression on endothelial cells of hematopoietic organs may be essential in the initial step of the homing process.

Published
1996

8. Constitutive expression of E-selectin and vascular cell adhesion molecule-1 on endothelial cells of hematopoietic tissues.

Author

Schweitzer KM, Dräger AM, van der Valk P, Thijsen SF, Zevenbergen A, Theijsmeijer AP, van der Schoot CE, and Langenhuijsen MM

Subjects
Adult, Antibodies, Monoclonal, Antibody Specificity, Antigens, CD34, Base Sequence, Bone Marrow embryology, Cell Adhesion physiology, Cells, Cultured, E-Selectin genetics, Endothelium, Vascular cytology, Endothelium, Vascular physiology, Hematopoietic Stem Cells immunology, Hematopoietic Stem Cells physiology, Hematopoietic System blood supply, Hematopoietic System cytology, Humans, Infant, Newborn, Liver embryology, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Messenger metabolism, Spleen embryology, Vascular Cell Adhesion Molecule-1 genetics, E-Selectin metabolism, Endothelium, Vascular metabolism, Hematopoietic System metabolism, Vascular Cell Adhesion Molecule-1 metabolism
Abstract

The first step in the homing of hematopoietic progenitor cells (HPCs) from the peripheral blood to the bone marrow involves an adhesion molecule-dependent contact with human bone marrow endothelial cells (HBMECs). In the present study we describe the constitutive expression of two of these molecules, E-selectin and vascular cell adhesion molecule-1 (VCAM-1), on endothelial cells of hematopoietic tissues. Immunophenotypic analysis of tissue sections of hematopoietically active (human adult and fetal bone marrow, fetal spleen, fetal liver, and adult spleen with extramedullary hematopoiesis) and inactive tissues (human adult spleen, lymph node, appendix, and liver; and fetal lung and fetal intestine) revealed that E-selectin and VCAM-1 are selectively expressed on endothelial cells of adult and fetal hematopoietic organs. These results were validated by means of reverse-transcriptase polymerase chain reaction. Adhesion studies revealed that binding of normal mobilized peripheral blood HPCs to HBMECs was completely inhibited by preincubation of HBMECs with anti-E-selectin (ENA2), whereas no effect of anti-VCAM-1 (1G11B1) was detected. These results suggest that E-selectin plays a role in the homing of HPCs and that its constitutive expression on endothelial cells of hematopoietic organs may be essential in the initial step of the homing process.

Published
1996

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