Search

Your search keyword '"Thanwisai, Aunchalee"' showing total 213 results
Start Over Author "Thanwisai, Aunchalee"
213 results on '"Thanwisai, Aunchalee"'

9. Global analysis of biosynthetic gene clusters reveals conserved and unique natural products in entomopathogenic nematode-symbiotic bacteria

Author

Shi, Yi-Ming, Hirschmann, Merle, Shi, Yan-Ni, Ahmed, Shabbir, Abebew, Desalegne, Tobias, Nicholas J., Grün, Peter, Crames, Jan J., Pöschel, Laura, Kuttenlochner, Wolfgang, Richter, Christian, Herrmann, Jennifer, Müller, Rolf, Thanwisai, Aunchalee, Pidot, Sacha J., Stinear, Timothy P., Groll, Michael, Kim, Yonggyun, and Bode, Helge B.

Published
2022
Full Text
View/download PDF

12. Genomic Landscape Reveals Chromosomally-Mediated Antimicrobial Resistome and Virulome of a High-Risk International Clone II Acinetobacter baumannii AB073 from Thailand

Author

Cheewapat, Rattiya, primary, Redkimned, Jadsadaporn, additional, Lekuthai, Sirikran, additional, Kitti, Thawatchai, additional, Tasanapak, Kannipa, additional, Thanwisai, Aunchalee, additional, Sitthisak, Sutthirat, additional, Sornda, Thanet, additional, Impheng, Hathaichanok, additional, Onsurathum, Sudarat, additional, Leungtongkam, Udomluk, additional, Lamlertthon, Supaporn, additional, Kucharoenphaibul, Siriwat, additional, Wongwigkarn, Jintana, additional, Singkum, Pantira, additional, Chanchaithong, Pattrarat, additional, and Thummeepak, Rapee, additional

Published
2024
Full Text
View/download PDF

14. A Photorhabdus Natural Product Inhibits Insect Juvenile Hormone Epoxide Hydrolase

Author

Nollmann, Friederike I, Heinrich, Antje K, Brachmann, Alexander O, Morisseau, Christophe, Mukherjee, Krishnendu, Casanova-Torres, Ángel M, Strobl, Frederic, Kleinhans, David, Kinski, Sebastian, Schultz, Katharina, Beeton, Michael L, Kaiser, Marcel, Chu, Ya-Yun, Ke, Long Phan, Thanwisai, Aunchalee, Bozhüyük, Kenan AJ, Chantratita, Narisara, Götz, Friedrich, Waterfield, Nick R, Vilcinskas, Andreas, Stelzer, Ernst HK, Goodrich-Blair, Heidi, Hammock, Bruce D, and Bode, Helge B

Subjects
Biological Sciences, Medicinal and Biomolecular Chemistry, Chemical Sciences, Infection, Animals, Biological Products, Enzyme Inhibitors, Epoxide Hydrolases, Insecta, Photorhabdus, Structure-Activity Relationship, Urea, biosynthesis, entomopathogenic bacteria, juvenile hormone epoxide hydrolase inhibitor, natural products, Biochemistry and Cell Biology, Organic Chemistry, Biochemistry and cell biology, Medicinal and biomolecular chemistry
Abstract

Simple urea compounds ("phurealipids") have been identified from the entomopathogenic bacterium Photorhabdus luminescens, and their biosynthesis was elucidated. Very similar analogues of these compounds have been previously developed as inhibitors of juvenile hormone epoxide hydrolase (JHEH), a key enzyme in insect development and growth. Phurealipids also inhibit JHEH, and therefore phurealipids might contribute to bacterial virulence.

Published
2015

15. An entomological survey of phlebotomine sand flies (Diptera: Psychodidae) in different areas of Thailand.

Author

Polseela, Raxsina, Vitta, Apichat, Thammeepak, Rapee, and Thanwisai, Aunchalee

Subjects
SAND flies, PSYCHODIDAE, DIPTERA, PHLEBOTOMUS, INSECT traps, HABITATS
Abstract

Sand flies (Diptera: Psychodidae) are significant medical vectors of pathogens that cause illness in humans and animals. The most significant of these is the genus Leishmania (Kinetoplastida: Trypanosomatidae). Leishmaniasis is an important vector-borne disease. They are spread throughout the Old and New Worlds by the bites of female phlebotomine sand flies. Despite this importance, certain provinces where autochthonous leishmaniasis has historically existed and is re-emerging are lacking current data regarding their sand fly fauna. To provide a current update on the local sand fly fauna, a comprehensive entomological survey encompassing eight provinces was conducted subsequent to an analysis of past data regarding the species composition and distribution of sand flies in the area. Thus, the purpose of this study was to ascertain the species composition in various locations across eight provinces spread across four regions in Thailand. In order to gather information about the distribution of the phlebotomine sand fly, researchers used modified Centers for Disease Control and Prevention light traps between February 2021 and February 2022. At these 30 trappings in each province, we conducted 240 trapping nights in total. Collected sand flies were morphologically identified. The adult sand flies collected in this study were 8314 and represented 24 species of four genera, including 16 of Sergentomyia, 6 of Phlebotomus, 1 of Idiophlebotomus, and 1 of Chinius. Among these species, Sergentomyia anodontis had the largest population (25.71%), and population peaks were observed in Uthaithani province (51.81%). The collected sand flies' male-to-female ratio was 1.21:1.00 (4,556:3,758). The study also discovered that Phlebotomus argentipes, a vector of Leishmania spp., was captured in Phayao province, and Phlebotomus major major was majorly found in Uthaithani province. The widespread distribution of sand fly populations in this study may indicate the epidemiologic importance of vector ecology in this habitat. Our results confirm the presence of several sand fly species in different provinces of Thailand and provide valuable information about the distribution and behavior of phlebotomine sand flies, which can be used to develop effective control strategies for the prevention of sand fly-borne diseases. In several Thai provinces, the study also tracked the nocturnal movements of both male and female sand flies. The results showed that phlebotomine sand flies are more active at night, peaking just after dusk. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

16. Entomopathogenic nematodes isolated from agricultural areas of Thailand and their activity against the larvae of Aedes aegypti, Aedes albopictus and Culex quinquefasciatus (Diptera: Culicidae)

Author

Ardpairin, Jiranun, primary, Subkrasae, Chanakan, additional, Dumidae, Abdulhakam, additional, Janthu, Pichamon, additional, Meesil, Wipanee, additional, Muangpat, Paramaporn, additional, Tandhavanant, Sarunporn, additional, Thanwisai, Aunchalee, additional, and Vitta, Apichat, additional

Published
2023
Full Text
View/download PDF

18. Entomopathogenic Nematodes and Their Symbiotic Bacteria from the National Parks of Thailand and Larvicidal Property of Symbiotic Bacteria against Aedes aegypti and Culex quinquefasciatus

Author

Thanwisai, Aunchalee, primary, Muangpat, Paramaporn, additional, Meesil, Wipanee, additional, Janthu, Pichamon, additional, Dumidae, Abdulhakam, additional, Subkrasae, Chanakan, additional, Ardpairin, Jiranun, additional, Tandhavanant, Sarunporn, additional, Yoshino, Timothy P., additional, and Vitta, Apichat, additional

Published
2022
Full Text
View/download PDF

20. Genome analysis of secondary metabolite‑biosynthetic gene clusters of Photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria

Author

Muangpat, Paramaporn, primary, Meesil, Wipanee, additional, Ngoenkam, Jatuporn, additional, Teethaisong, Yothin, additional, Thummeepak, Rapee, additional, Sitthisak, Sutthirat, additional, Tandhavanant, Sarunporn, additional, Chantratita, Narisara, additional, Bode, Helge B., additional, Vitta, Apichat, additional, and Thanwisai, Aunchalee, additional

Published
2022
Full Text
View/download PDF

28. Global analysis of biosynthetic gene clusters reveals conserved and unique natural products in entomopathogenic nematode-symbiotic bacteria

Author

Shi, Yi-Ming, primary, Hirschmann, Merle, additional, Shi, Yan-Ni, additional, Ahmed, Shabbir, additional, Abebew, Desalegne, additional, Tobias, Nicholas J., additional, Grün, Peter, additional, Crames, Jan J., additional, Pöschel, Laura, additional, Kuttenlochner, Wolfgang, additional, Richter, Christian, additional, Herrmann, Jennifer, additional, Müller, Rolf, additional, Thanwisai, Aunchalee, additional, Pidot, Sacha J., additional, Stinear, Timothy P., additional, Groll, Michael, additional, Kim, Yonggyun, additional, and Bode, Helge B., additional

Published
2022
Full Text
View/download PDF

32. Genetic analysis of a 66-kDa protein-encoding gene of Angiostrongylus cantonensis and Angiostrongylus malaysiensis.

Author

Dumidae, Abdulhakam, Subkrasae, Chanakan, Ardpairin, Jiranun, Thanwisai, Aunchalee, and Vitta, Apichat

Subjects
ANGIOSTRONGYLUS cantonensis, GENETIC variation, LIFE cycles (Biology), AMINO acid sequence, GENETIC markers, TOXAPHENE
Abstract

The rat lungworm Angiostrongylus cantonensis is globally known to be the cause of oeosinophilic meningitis in humans. Another congener, Angiostrongylus malaysiensis , is closely related to A. cantonensis and has been described as a potential human pathogenic parasite. These 2 worms are similar in terms of life cycle, host range and morphological and genetic information. However, there are limited studies about their genetic diversity based on the 66-kDa protein-encoding gene. The objective of this study was to explore the 66-kDa protein sequence variation of A. cantonensis and A. malaysiensis collected from Thailand. Two adult and 53 third-stage larval specimens of Angiostrongylus from 4 geographic locations in Thailand were molecularly identified using the 66-kDa protein gene. The phylogenetic trees (Bayesian inference tree and maximum-likelihood tree) showed that Angiostrongylus formed a monophyletic clade with a clear separation between A. cantonensis and A. malaysiensis. The genetic distance between A. cantonensis and A. malaysiensis varies from 0.82 to 2.86%, with a total of 16 variable sites. The analysis of genetic diversity revealed 1 and 5 new haplotypes of A. cantonensis and A. malaysiensis , respectively, and showed genetic differences between the populations of A. cantonensis and A. malaysiensis. The haplotype networks of A. cantonensis and A. malaysiensis populations in Thailand are similar to those of populations in some countries, indicating the range expansion of genomic origin between populations in different areas. In conclusion, the 66-kDa protein gene was a good genetic marker for studying genetic diversity and discriminating between A. cantonensis and A. malaysiensis. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

33. Insight into Molecular Epidemiology, Antimicrobial Resistance, and Virulence Genes of Extensively Drug-Resistant Acinetobacter baumannii in Thailand

Author

Kongthai, Phattaraporn, primary, Thummeepak, Rapee, additional, Leungtongkam, Udomluk, additional, Pooarlai, Renuka, additional, Kitti, Thawatchai, additional, Thanwisai, Aunchalee, additional, Chantratita, Narisara, additional, Millard, Andrew D., additional, and Sitthisak, Sutthirat, additional

Published
2021
Full Text
View/download PDF

35. Photorhabdus heterorhabditis subsp. aluminescens subsp. nov., Photorhabdus heterorhabditis subsp. heterorhabditis subsp. nov., Photorhabdus australis subsp. thailandensis subsp. nov., Photorhabdus australis subsp. australis subsp. nov., and Photorhabdus aegyptia sp. nov. isolated from Heterorhabditis entomopathogenic nematodes

Author

Machado, Ricardo A. R., primary, Muller, Arthur, additional, Ghazal, Shimaa M., additional, Thanwisai, Aunchalee, additional, Pagès, Sylvie, additional, Bode, Helge B., additional, Hussein, Mona A., additional, Khalil, Kamal M., additional, and Tisa, Louis S., additional

Published
2021
Full Text
View/download PDF

40. Identification of entomopathogenic nematodes and their symbiotic bacteria in national parks of Thailand, and mosquitocidal activity of Xenorhabdus griffiniae against Aedes aegypti larvae.

Author

Thanwisai, Aunchalee, Muangpat, Paramaporn, Dumidae, Abdulhakam, Subkrasae, Chanakan, Ardpairin, Jiranun, Tandhavanant, Sarunporn, and Vitta, Apichat

Subjects
*INSECT nematodes, *AEDES aegypti, *XENORHABDUS, *NATIONAL parks & reserves, *GREATER wax moth, *INSECT pest control, *LARVAE
Abstract

Summary: Entomopathogenic nematodes (EPN) Steinernema and Heterorhabditis with symbionts with Xenorhabdus and Photorhabdus bacteria, respectively, are reported as biocontrol agents for insect control. The objectives of this study were to identify EPN and their symbiotic bacteria in national parks of Phitsanulok Province, Thailand, and to test how Xenorhabdus isolates attack Aedes aegypti larvae. We collected 810 soil samples from four national parks. The juvenile stage of EPN was isolated from soil samples using a baiting technique with Galleria mellonella followed by a White trap. Partial regions of 28S rDNA and internal transcript spacer were sequenced to identify EPN, and recA sequencing was used to discriminate between Xenorhabdus and Photorhabdus. We found that 74 of the 810 soil samples (9.1%) were positive for the EPN. The EPN were molecularly identified as S. surkhetense , S. longicaudum , H. indica and Heterorhabditis sp. SGmg3, and their symbiotic bacteria were identified as X. stockiae , X. griffiniae , X. indica , X. vietnamensis , P. luminescens subsp. akhurstii , and P. temperata subsp. temperata. Xenorhabdus griffiniae showed potential larvicidal activity against A. aegypti (91% mortality at 72 and 96 h after exposure). This study demonstrates the diversity of EPN and symbiotic bacteria in national parks of Thailand and the potential to use X. griffiniae as a biocontrol agent to kill A. aegypti larvae. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

41. Focused natural product elucidation by prioritizing high-throughput metabolomic studies with machine learning

Author

Tobias, Nicholas J., Parra-Rojas, César, Shi, Yan-Ni, Shi, Yi-Ming, Simonyi, Svenja, Thanwisai, Aunchalee, Vitta, Apichat, Chantratita, Narisara, Hernández-Vargas, Esteban A., Bode, Helge Björn, Tobias, Nicholas J., Parra-Rojas, César, Shi, Yan-Ni, Shi, Yi-Ming, Simonyi, Svenja, Thanwisai, Aunchalee, Vitta, Apichat, Chantratita, Narisara, Hernández-Vargas, Esteban A., and Bode, Helge Björn

Abstract

Bacteria of the genera Photorhabdus and Xenorhabdus produce a plethora of natural products to support their similar symbiotic lifecycles. For many of these compounds, the specific bioactivities are unknown. One common challenge in natural product research when trying to prioritize research efforts is the rediscovery of identical (or highly similar) compounds from different strains. Linking genome sequence to metabolite production can help in overcoming this problem. However, sequences are typically not available for entire collections of organisms. Here we perform a comprehensive metabolic screening using HPLC-MS data associated with a 114-strain collection (58 Photorhabdus and 56 Xenorhabdus) from across Thailand and explore the metabolic variation among the strains, matched with several abiotic factors. We utilize machine learning in order to rank the importance of individual metabolites in determining all given metadata. With this approach, we were able to prioritize metabolites in the context of natural product investigations, leading to the identification of previously unknown compounds. The top three highest-ranking features were associated with Xenorhabdus and attributed to the same chemical entity, cyclo(tetrahydroxybutyrate). This work addresses the need for prioritization in high-throughput metabolomic studies and demonstrates the viability of such an approach in future research.

Published
2019

43. Cyclo(tetrahydroxybutyrate) production is sufficient to distinguish between Xenorhabdus and Photorhabdus isolates in Thailand

Author

Tobias, Nicholas J., primary, Parra‐Rojas, César, additional, Shi, Yan‐Ni, additional, Shi, Yi‐Ming, additional, Simonyi, Svenja, additional, Thanwisai, Aunchalee, additional, Vitta, Apichat, additional, Chantratita, Narisara, additional, Hernandez‐Vargas, Esteban A., additional, and Bode, Helge B., additional

Published
2019
Full Text
View/download PDF

45. Effect of colony morphology variation of Burkholderia pseudomallei on intracellular survival and resistance to antimicrobial environments in human macrophages in vitro

Author

Limmathurotsakul Direk, Thanwisai Aunchalee, Tandhavanant Sarunporn, Korbsrisate Sunee, Day Nicholas PJ, Peacock Sharon J, and Chantratita Narisara

Subjects
Microbiology, QR1-502
Abstract

Abstract Background Primary diagnostic cultures from patients with melioidosis demonstrate variation in colony morphology of the causative organism, Burkholderia pseudomallei. Variable morphology is associated with changes in the expression of a range of putative virulence factors. This study investigated the effect of B. pseudomallei colony variation on survival in the human macrophage cell line U937 and under laboratory conditions simulating conditions within the macrophage milieu. Isogenic colony morphology types II and III were generated from 5 parental type I B. pseudomallei isolates using nutritional limitation. Survival of types II and III were compared with type I for all assays. Results Morphotype was associated with survival in the presence of H2O2 and antimicrobial peptide LL-37, but not with susceptibility to acid, acidified sodium nitrite, or resistance to lysozyme, lactoferrin, human neutrophil peptide-1 or human beta defensin-2. Incubation under anaerobic conditions was a strong driver for switching of type III to an alternative morphotype. Differences were noted in the survival and replication of the three types following uptake by human macrophages, but marked strain-to strain-variability was observed. Uptake of type III alone was associated with colony morphology switching. Conclusions Morphotype is associated with phenotypes that alter the ability of B. pseudomallei to survive in adverse environmental conditions.

Published
2010
Full Text
View/download PDF

49. Screening of the Antimicrobial Activity against Drug Resistant Bacteria of Photorhabdus and Xenorhabdus Associated with Entomopathogenic Nematodes from Mae Wong National Park, Thailand

Author

Muangpat, Paramaporn, primary, Yooyangket, Temsiri, additional, Fukruksa, Chamaiporn, additional, Suwannaroj, Manawat, additional, Yimthin, Thatcha, additional, Sitthisak, Sutthirat, additional, Chantratita, Narisara, additional, Vitta, Apichat, additional, Tobias, Nicholas J., additional, Bode, Helge B., additional, and Thanwisai, Aunchalee, additional

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results