33 results on '"Théate I"'
Search Results
2. Tumoral immune resistance based on tryptophan degradation by indoleamine 2,3-dioxygenase
- Author
-
Van den Eynde, B.J., Théate, I., Uyttenhove, C., Colau, D., Pilotte, L., and Stroobant, V.
- Published
- 2007
- Full Text
- View/download PDF
3. Hidrocystomatose apocrine transitoire du scrotum
- Author
-
Cuvelier, M., primary, Lasek, A., additional, Ngendahayo, P., additional, Théate, I., additional, Sass, U., additional, Roquet-Gravy, P.-P., additional, and Bulinckx, A., additional
- Published
- 2020
- Full Text
- View/download PDF
4. Detection, identification and in vivo treatment responsiveness of bone morphogenetic protein (BMP)-activated cell populations in the synovium of patients with rheumatoid arthritis
- Author
-
Verschueren, P C P M, Lories, R J U, Daans, M, Théate, I, Durez, P, Westhovens, R, and Luyten, F P
- Published
- 2009
- Full Text
- View/download PDF
5. Unusual Localisation for Onychomatricoma on the 5th Toenail: A Case Report and Review of the Literature
- Author
-
Coutellier, A., Théate, I., and Vanhooteghem, O.
- Subjects
Article Subject - Abstract
Onychomatricoma is a rare and benign tumour of the nail matrix but originates rarely from the ventral portion of the proximal nail fold. This tumour is characterised by fingerlike projections that invade the nail plate. This lesion, of unknown aetiology, is typically asymptomatic with slow progression. Localisation on the finger is the most frequently described. We report the case of a 68-year-old woman who has an onychomatricoma in an unusual location, the fifth toe of the left foot. Due to its clinical appearance, the tumour can be confused with and treated as onychomycosis. However, if it is resistant to an oral antifungal well behaved treatment, one must consider onychomatricoma diagnosis.
- Published
- 2016
- Full Text
- View/download PDF
6. Granulomes annulaires profonds multiples céphaliques de l’enfant
- Author
-
Bulinckx, A., primary, Cambier, N., additional, Wayllace Gaspar, L., additional, Théate, I., additional, Roquet-Gravy, P.P., additional, and Bessis, D., additional
- Published
- 2017
- Full Text
- View/download PDF
7. Granulomes annulaires profonds multiples céphaliques de l’enfant
- Author
-
Bulinckx, A., primary, Cambier, N., additional, Wayllace Gaspar, L., additional, Théate, I., additional, Roquet-Gravy, P.-P., additional, and Bessis, D., additional
- Published
- 2014
- Full Text
- View/download PDF
8. Detection, identification and in vivo treatment responsiveness of bone morphogenetic protein (BMP)-activated cell populations in the synovium of patients with rheumatoid arthritis
- Author
-
Verschueren, P C P M, primary, Lories, R J U, additional, Daans, M, additional, Théate, I, additional, Durez, P, additional, Westhovens, R, additional, and Luyten, F P, additional
- Published
- 2008
- Full Text
- View/download PDF
9. CLINICAL AND PATHOLOGICAL FEATURES OF 14 NON-HODGKINʼS LYMPHOMAS ASSOCIATED WITH COELIAC DISEASE
- Author
-
Sonet, A., primary, Théate, I., additional, Delos, M., additional, Montfort, L., additional, Mineur, P., additional, Driesschaert, P., additional, Michaux, L., additional, Ferrant, A., additional, and Bosly, A., additional
- Published
- 2004
- Full Text
- View/download PDF
10. Les lésions spécifiques cutanées dans la leucémie myélomonocytaire chronique : un spectre de proliférations de cellules myélomonocytaires et dendritiques. Étude de 42 cas
- Author
-
Vitte, F., Fabiani, B., Bénet, C., Dalac, S., Balme, B., Delattre, C., Vergier, B., Beylot-Barry, M., Vignon-Pennamen, D., Ortonne, N., Algros, M.-P., Carlotti, A., Samaleire, D., Frouin, E., Levy, A., Laroche, L., Theate, I., Monnien, F., Mugneret, F., and Petrella, T.
- Published
- 2012
- Full Text
- View/download PDF
11. [Recycled formalin: A new environmental mitigation tool in pathology?]
- Author
-
Chicaud M, Vergara R, Théate I, Lesne P, Rullier A, and le Teap P
- Subjects
- Humans, France, Pathology methods, Pathology, Clinical methods, Formaldehyde, Recycling, Fixatives
- Abstract
Formalin is the international gold-standard fixative in pathology laboratories. However it is not the ideal one considering its deleterious effects on individuals and the environment. Complete formalin removal or even substitution does not seem possible in the near future. In this update, we present various tools allowing to integrate the use of formalin into an ecocare approach. Among them, formalin recycling according to the protocol developed by the University Hospital of Bordeaux is simple to implement and delivers rapid and significant results, allowing pathology professionals to meet the sustainable development objectives included in the France 2030 agenda., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
12. [Nodular lymphocyte predominant Hodgkin lymphoma (paragranuloma of Poppema) in children: Case report, review of the literature and treatment].
- Author
-
Wilgenhof K, Théate I, Devalck C, Forsyth R, and Dehou MF
- Subjects
- Male, Humans, Child, Prognosis, Lymphocytes pathology, Hodgkin Disease diagnosis, Hodgkin Disease therapy, Hodgkin Disease pathology
- Abstract
We present the case of a 12 year old child with a limp. The diagnostic work-up reveals splenomegaly, multifocal bone involvement and abdominal adenopathies. A biopsy of an intra-abdominal lesion shows a lymphoid mass with a nodular architecture composed of poorly defined nodules. We identify large cells with irregular, sometimes poly-lobulated nuclei with a particular immunohistochemical profile. Those "pop-corn" cells are positive for CD20, CD79a, pax-5 and bcl-6 and are negative for CD15, CD30, bcl-2, TdT, CD56 and EMA. There is a diffuse follicular helper T cell population that is located in between the tumour cells. The overall picture is indicative of a nodular lymphocyte predominant Hodgkin lymphoma. Advanced stage of this disease is rare in children and there is currently little data to guide optimal treatment. Because of a stage IV disease, the patient is treated with chemotherapy after which complete metabolic remission is observed. 3.5 years after the initial diagnosis, our patient relapses. He is treated with chemotherapy and an autologous peripheral blood stem cell transplantation. He remains in complete remission since then. This case illustrates the favorable prognosis of the disease even after relapse., (Copyright © 2022 Elsevier Masson SAS. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
13. A Single Nail Dystrophy as a Unique Manifestation of Sarcoidosis: A Case Report.
- Author
-
Mengeot L, Stallenberg B, Théate I, and Vanhooteghem O
- Abstract
Sarcoidosis with nail involvement is rare and most commonly affecting plural digits. Nail changes are frequently an indication of systemic disease and underlying bone involvement, thus complete clinical evaluation with bone and thorax radiological examination is a necessity in suspected cases. We report a case of onychodystrophy with osseous involvement of only one finger as unique manifestation of sarcoidosis, which is very rare., Competing Interests: All authors certified that they have no conflicts of interest, including specific financial interests and relationships and affiliations relevant to the subject of the manuscript., (Copyright © 2021 by S. Karger AG, Basel.)
- Published
- 2022
- Full Text
- View/download PDF
14. A rapid unfavorable penile calciphylaxis case followed by total penectomy.
- Author
-
Roquet-Gravy C, Théate I, Lejeune M, and Vanhooteghem O
- Abstract
When the diagnosis of penile calciphylaxis is suggested, the evolution of the disease is rapidly unfavorable; in this case, a rapid medical treatment must be established to obtain an improvement of the disease to avoid penectomy., Competing Interests: All authors certified that they have no conflicts of interest, including specific financial interests and relationships and affiliations relevant to the subject of this manuscript., (© 2021 The Authors. Clinical Case Reports published by John Wiley & Sons Ltd.)
- Published
- 2021
- Full Text
- View/download PDF
15. [Transitory apocrine hidrocystomatosis of the scrotum].
- Author
-
Cuvelier M, Lasek A, Ngendahayo P, Théate I, Sass U, Roquet-Gravy PP, and Bulinckx A
- Subjects
- Humans, Infant, Male, Apocrine Glands, Genital Neoplasms, Male pathology, Hidrocystoma pathology, Scrotum, Sweat Gland Neoplasms pathology
- Abstract
Background: Apocrine hidrocystomas are benign cystic tumors that develop from apocrine gland proliferation. In most cases, they are translucent solitary lesions of the face, generally found in the periorbital region, on the scalp or on the neck. More rarely, apocrine hidrocystomas may be multiple and appear on the ears, trunk, shoulders and genital area. They generally appear in adulthood, with only a few pediatric cases being reported, of which three in the genital area, with a solitary case of multiple hidrocystomas of the scrotum, although no cases of spontaneous involution of hidrocystomas have previously been reported., Patients and Methods: Two boys aged 4 and 6 months were seen in consultation for small sub-millimeter size, subcutaneous, black lesions on the scrotum that appeared in the weeks following birth. Histological examination of these lesions resulted in a diagnosis of apocrine hidrocystoma. The children were seen again a few weeks later and the skin lesions had totally disappeared. We report two cases of multiple apocrine hidrocystomas on the scrotum with spontaneous involution diagnosed in a 4- and a 6-month-old boy., Discussion: Apocrine hidrocystomas are rare benign adnexal tumors that develop from apocrine sweat glands. They are considered as cystic proliferations of the apocrine glands rather than simple retention cysts. The main differential diagnosis of the rare cases of multiple apocrine hidrocystomas are eccrine hidrocystomas. The treatment of such lesions is based on surgical excision if they are isolated, daily application of topical atropine 1%, or CO
2 laser for multiple apocrine hidrocystomas., (Copyright © 2019 Elsevier Masson SAS. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
16. Association of PDGFRB Mutations With Pediatric Myofibroma and Myofibromatosis.
- Author
-
Dachy G, de Krijger RR, Fraitag S, Théate I, Brichard B, Hoffman SB, Libbrecht L, Arts FA, Brouillard P, Vikkula M, Limaye N, and Demoulin JB
- Abstract
Importance: Myofibroma is the most frequent fibrous tumor in children. Multicentric myofibroma (referred to as infantile myofibromatosis) is a life-threatening disease., Objective: To determine the frequency, spectrum, and clinical implications of mutations in the PDGFRB receptor tyrosine kinase found in sporadic myofibroma and myofibromatosis., Design, Setting, and Participants: In this retrospective study of 69 patients with sporadic myofibroma or myofibromatosis, 85 tumor samples were obtained and analyzed by targeted deep sequencing of PDGFRB. Mutations were confirmed by an alternative method of sequencing and were experimentally characterized to confirm gain of function and sensitivity to the tyrosine kinase inhibitor imatinib., Main Outcomes and Measures: Frequency of gain-of-function PDGFRB mutations in sporadic myofibroma and myofibromatosis. Sensitivity to imatinib, as assessed experimentally., Results: Of the 69 patients with tumor samples (mean [SD] age, 7.8 [12.7] years), 60 were children (87%; 29 girls [48%]) and 9 were adults (13%; 4 women [44%]). Gain-of-function PDGFRB mutations were found in samples from 25 children, with no mutation found in samples from adults. Mutations were particularly associated with severe multicentric disease (13 of 19 myofibromatosis cases [68%]). Although patients had no familial history, 3 of 25 mutations (12%) were likely to be germline, suggesting de novo heritable alterations. All of the PDGFRB mutations were associated with ligand-independent receptor activation, and all but one were sensitive to imatinib at clinically relevant concentrations., Conclusions and Relevance: Gain-of-function mutations of PDGFRB in myofibromas may affect only children and be more frequent in the multicentric form of disease, albeit present in solitary pediatric myofibromas. These alterations may be sensitive to tyrosine kinase inhibitors. The PDGFRB sequencing appears to have a high value for diagnosis, prognosis, and therapy of soft-tissue tumors in children.
- Published
- 2019
- Full Text
- View/download PDF
17. [Multiple cephalic deep granuloma annulare of children].
- Author
-
Bulinckx A, Cambier N, Wayllace Gaspar L, Théate I, Roquet-Gravy PP, and Bessis D
- Subjects
- Child, Child, Preschool, Female, Humans, Infant, Male, Granuloma Annulare pathology, Head
- Abstract
Background: Deep granuloma annulare is a fairly rare variety of granuloma annulare. It is seen predominantly in children and mainly affects the anterior aspect of the legs and the top of the feet; cephalic presentation is rare. Below, we report three cases of deep granuloma annulare in children presenting solely at the cephalic extremity., Patients and Methods: Case 1: a six-year-old boy presented 7 cutaneous nodules measuring 1 to 2cm that were flesh-coloured, insensitive to palpation, of hard consistency and deeply attached. The lesions were grouped together on the anterior half of the left temporal fossa. While spontaneous regression of the three nodules was noted in the month following cutaneous biopsy, these nodules recurred a few months later. Case 2: a four-year-old girl with five deep cephalic nodules measuring around one centimetre and the colour of normal skin were seen on her right temporal fossa. The child was lost to follow-up after biopsy. Case 3: a four-month-old infant was presenting some 15 deep cutaneous nodules arranged in linear fashion on the forehead next to the left temporal fossa. These nodules regressed spontaneously one month after biopsy. In all three cases, histological examination confirmed the diagnosis of deep granuloma annulare., Discussion: There have been few published cases of multiple, cephalic, deep granuloma annulare at a single site in children. The condition has an extensive differential diagnosis that includes malignant tumours; in addition, histological confirmation is normally essential. Treatment is not qualified and therapeutic extension with clinical monitoring alone may frequently be recommended., (Copyright © 2017 Elsevier Masson SAS. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
18. Genetic differences between paediatric and adult Burkitt lymphomas.
- Author
-
Havelange V, Pepermans X, Ameye G, Théate I, Callet-Bauchu E, Barin C, Penther D, Lippert E, Michaux L, Mugneret F, Dastugue N, Raphaël M, Vikkula M, and Poirel HA
- Subjects
- Adolescent, Adult, Age Factors, Child, Child, Preschool, Female, Humans, Infant, Male, Burkitt Lymphoma genetics, Chromosome Aberrations, Chromosomes, Human genetics, Loss of Heterozygosity, Neoplasm Proteins genetics
- Abstract
Dysregulation of MYC is the genetic hallmark of Burkitt lymphoma (BL) but it is encountered in other aggressive mature B-cell lymphomas. MYC dysregulation needs other cooperating events for BL development. We aimed to characterize these events and assess the differences between adult and paediatric BLs that may explain the different outcomes in these two populations. We analysed patterns of genetic aberrations in a series of 24 BLs: 11 adults and 13 children. We looked for genomic imbalances (copy number variations), copy-neutral loss of heterozygosity (CN-LOH) and mutations in TP53, CDKN2A, ID3 (exon 1), TCF3 (exon17) and CCND3 (exon 6). Young patients displayed more frequent 13q31.3q32.1 amplification, 7q32q36 gain and 5q23.3 CN-LOH, while 17p13 and 18q21.3 CN-LOH were only detected in adult BLs. ID3 mutations were present in all adult samples, but only in 42% of childhood cases. CCND3 and ID3 double-hit mutations, as well as 18q21 CN-LOH, seemed to be associated with poorer outcome. For the first time, we report different genetic anomalies between adult and paediatric BLs, suggesting age-related heterogeneity in Burkitt lymphomagenesis. This may explain the poorer prognosis of adult BLs. Additional studies are needed to confirm these results in the setting of clinical trials., (© 2016 John Wiley & Sons Ltd.)
- Published
- 2016
- Full Text
- View/download PDF
19. The peculiar 11q-gain/loss aberration reported in a subset of MYC-negative high-grade B-cell lymphomas can also occur in a MYC-rearranged lymphoma.
- Author
-
Havelange V, Ameye G, Théate I, Callet-Bauchu E, Lippert E, Luquet I, Raphaël M, Vikkula M, and Poirel HA
- Subjects
- Adult, Child, Child, Preschool, Humans, Karyotyping, Male, Chromosome Aberrations, Chromosomes, Human, Pair 11, Gene Rearrangement, Genes, myc, Lymphoma, B-Cell genetics
- Published
- 2016
- Full Text
- View/download PDF
20. Hepatosplenic γδ T-cell lymphoma after allogeneic bone marrow transplantation.
- Author
-
Vekemans MC, Michaux L, Saussoy P, Van Den Neste E, Théate I, and Ferrant A
- Subjects
- Humans, Liver Neoplasms etiology, Lymphoma, T-Cell etiology, Male, Middle Aged, Splenic Neoplasms etiology, Transplantation, Homologous adverse effects, Bone Marrow Transplantation adverse effects, Liver Neoplasms diagnosis, Lymphoma, T-Cell diagnosis, Splenic Neoplasms diagnosis
- Published
- 2015
- Full Text
- View/download PDF
21. Extensive profiling of the expression of the indoleamine 2,3-dioxygenase 1 protein in normal and tumoral human tissues.
- Author
-
Théate I, van Baren N, Pilotte L, Moulin P, Larrieu P, Renauld JC, Hervé C, Gutierrez-Roelens I, Marbaix E, Sempoux C, and Van den Eynde BJ
- Subjects
- Animals, Antibodies, Monoclonal biosynthesis, Antibodies, Monoclonal immunology, Biomarkers, Tumor genetics, Biomarkers, Tumor immunology, Cells, Cultured, Dendritic Cells immunology, Female, Gene Expression Profiling methods, Gene Expression Regulation, Neoplastic immunology, Genitalia, Female immunology, Humans, Indoleamine-Pyrrole 2,3,-Dioxygenase genetics, Indoleamine-Pyrrole 2,3,-Dioxygenase immunology, Lung immunology, Lymph Nodes immunology, Lymphatic Metastasis, Mice, Inbred BALB C, Neoplasm Proteins genetics, Neoplasm Proteins immunology, Neoplasms genetics, Placenta immunology, Pregnancy, RNA, Messenger genetics, Biomarkers, Tumor metabolism, Indoleamine-Pyrrole 2,3,-Dioxygenase metabolism, Neoplasm Proteins metabolism, Neoplasms immunology
- Abstract
Tryptophan catabolism by indoleamine 2,3-dioxygenase 1 (IDO1) plays a key role in tumoral resistance to immune rejection. In humans, constitutive expression of IDO1 has been observed in several tumor types. However, a comprehensive analysis of its expression in normal and tumor tissues is still required to anticipate the risks and potential benefits of IDO1 inhibitors. Using a newly validated monoclonal antibody to human IDO1, we performed an extensive immunohistochemical analysis of IDO1 expression in normal and tumor tissues. In normal tissues, IDO1 was expressed by endothelial cells in the placenta and lung and by epithelial cells in the female genital tract. In lymphoid tissues, IDO1 was expressed in mature dendritic cells with a phenotype (CD83(+), DC-LAMP(+), langerin(-), CD123(-), CD163(-)) distinct from plasmacytoid dendritic cells. Importantly, IDO1-expressing dendritic cells were not enriched in tumor-draining lymph nodes, in contrast with previously reported findings. IDO1-expressing cells were observed in a large fraction (505/866, 58%) of human tumors. They comprised tumor cells, endothelial cells, and stromal cells in proportions that varied depending on the tumor type. Tumors showing the highest proportions of IDO1-immunolabeled samples were carcinomas of the endometrium and cervix, followed by kidney, lung, and colon. This hierarchy of IDO1 expression was confirmed by gene expression data mined from The Cancer Genome Atlas database. Expression of IDO1 may be used to select tumors likely to benefit from targeted therapy with IDO1 inhibitors., (©2014 American Association for Cancer Research.)
- Published
- 2015
- Full Text
- View/download PDF
22. BMI1, the polycomb-group gene, is recurrently targeted by genomic rearrangements in progressive B-cell leukemia/lymphoma.
- Author
-
Rouhigharabaei L, Ferreiro JF, Put N, Michaux L, Tousseyn T, Lefebvre C, Gardiner A, De Kelver W, Demuynck H, Verschuere J, Théate I, Vicente C, Vandenberghe P, Cools J, and Wlodarska I
- Subjects
- Aged, Aged, 80 and over, Allelic Imbalance, Cyclin D1 genetics, Cyclin-Dependent Kinase Inhibitor p15 genetics, Cyclin-Dependent Kinase Inhibitor p16 genetics, Female, Humans, In Situ Hybridization, Fluorescence, Male, Middle Aged, Polymorphism, Single Nucleotide, Translocation, Genetic, Chromosome Aberrations, Leukemia, Lymphocytic, Chronic, B-Cell genetics, Lymphoma, B-Cell genetics, Polycomb Repressive Complex 1 genetics
- Abstract
BMI1, a Polycomb-group gene located at 10p12.2, is implicated in the pathogenesis of a variety of tumors. However, the genetic molecular mechanisms underlying its aberrant expression in cancer cells remain largely unknown. In this study, we show that BMI1 is recurrently targeted by chromosomal aberrations in B-cell leukemia/lymphoma. We identified a novel t(10;14)(p12;q32)/IGH-BMI1 rearrangement and its IGL variant in six cases of chronic lymphocytic leukemia (CLL) and found that these aberrations were consistently acquired at time of disease progression and high grade transformation of leukemia (Richter syndrome). The IG-BMI1 translocations were not associated with any particular molecular subtype of CLL and the leukemias were negative for common mutations of NOTCH1 and TP53, known to increase a risk of progression and transformation in CLL. In addition, using FISH and SNP array analysis, we identified a wide range of BMI1-involving 10p12 lesions in 17 cases of mantle cell lymphoma (MCL). These aberrations included various balanced and unbalanced structural abnormalities and very frequently but not exclusively, were associated with gain of the BMI1 locus and loss of the 10p terminal sequences. These findings point to genomic instability at the 10p region in MCL which likely promotes rearrangements and deregulation of BMI1. Our findings are in line with previously published observations correlating overexpression of BMI1 with tumor progression and chemoresistance. In summary, our study provides new insights into genetic molecular mechanisms underlying aberrant expression of BMI1 in lymphoma and documents its contribution in the pathogenesis of Richter syndrome and MCL., (Copyright © 2013 Wiley Periodicals, Inc.)
- Published
- 2013
- Full Text
- View/download PDF
23. Patterns of genomic aberrations suggest that Burkitt lymphomas with complex karyotype are distinct from other aggressive B-cell lymphomas with MYC rearrangement.
- Author
-
Havelange V, Ameye G, Théate I, Callet-Bauchu E, Mugneret F, Michaux L, Dastugue N, Penther D, Barin C, Collonge-Rame MA, Baranger L, Terré C, Nadal N, Lippert E, Laï JL, Cabrol C, Tigaud I, Herens C, Hagemeijer A, Raphael M, Libouton JM, and Poirel HA
- Subjects
- Abnormal Karyotype, Adolescent, Child, Child, Preschool, Female, Humans, In Situ Hybridization, Fluorescence, Male, Burkitt Lymphoma genetics, Chromosome Aberrations, Chromosomes, Human, Gene Rearrangement, Genes, myc, Lymphoma, B-Cell genetics
- Abstract
We previously showed that complex karyotypes (CK) and chromosome 13q abnormalities have an adverse prognostic impact in childhood Burkitt lymphomas/leukemias (BL) and diffuse large B-cell lymphomas (DLBCL). The aim of our study was to identify recurrent alterations associated with MYC rearrangements in aggressive B-cell lymphomas with CK. Multicolor fluorescence in situ hybridization (M-FISH) was performed in 84 patient samples (59 adults and 25 children), including 37 BL (13 lymphomas and 24 acute leukemias), 12 DLBCL, 28 B-cell lymphomas with intermediate features (DLBCL/BL), 4 B-cell precursor acute lymphoblastic leukemias (BCP-ALL), and 3 unclassifiable B-cell lymphomas. New (cytogenetically undetected) abnormalities were identified in 80% of patients. We also refined one-third of the chromosomal aberrations detected by karyotyping. M-FISH proved to be more useful in identifying chromosomal partners involved in unbalanced translocations and in revealing greater complexity of 13q rearrangements. Most of the newly identified or refined recurrent alterations involved 1q, 13q and 3q (gains/losses), 7q and 18q (gains), or 6q (losses), suggesting that these secondary aberrations may play a role in lymphomagenesis. Several patterns of genomic aberrations were identified: 1q gains in BL, trisomies 7 in DLBCL, and 18q-translocations in adult non-BL. BCP-ALL usually displayed an 18q21 rearrangement. BL karyotypes were less complex and aneuploid than those of other MYC-rearranged lymphomas. BCP-ALL and DLBCL/BL were associated with a higher rate of early death than BL and DLBCL. These findings support the categorization of DLBCL/BL as a distinct entity and suggest that BL with CK are indeed different from other aggressive MYC-rearranged lymphomas, which usually show greater genetic complexity. © 2012 Wiley Periodicals, Inc., (Copyright © 2012 Wiley Periodicals, Inc.)
- Published
- 2013
- Full Text
- View/download PDF
24. Neogenesis of lymphoid structures and antibody responses occur in human melanoma metastases.
- Author
-
Cipponi A, Mercier M, Seremet T, Baurain JF, Théate I, van den Oord J, Stas M, Boon T, Coulie PG, and van Baren N
- Subjects
- Antibodies, Neoplasm immunology, B-Lymphocytes immunology, Case-Control Studies, Genes, Immunoglobulin, Germinal Center immunology, Germinal Center pathology, Humans, Immunohistochemistry, Lymphoid Tissue pathology, Melanoma genetics, Melanoma pathology, Skin Neoplasms genetics, Skin Neoplasms pathology, Antibodies, Neoplasm biosynthesis, Lymphoid Tissue immunology, Melanoma immunology, Melanoma secondary, Skin Neoplasms immunology, Skin Neoplasms secondary
- Abstract
Lymphoid neogenesis, or the development of lymphoid structures in nonlymphoid organs, is frequently observed in chronically inflamed tissues, during the course of autoimmune, infectious, and chronic graft rejection diseases, in which a sustained lymphocyte activation occurs in the presence of persistent antigenic stimuli. The presence of such ectopic lymphoid structures has also been reported in primary lung, breast, and germline cancers, but not yet in melanoma. In this study, we observed ectopic lymphoid structures, defined as lymphoid follicles comprising clusters of B lymphocytes and follicular dendritic cells (DC), associated with high endothelial venules (HEV) and clusters of T cells and mature DCs, in 7 of 29 cutaneous metastases from melanoma patients. Some follicles contained germinal centers. In contrast to metastatic lesions, primary melanomas did not host follicles, but many contained HEVs, suggesting an incomplete lymphoid neogenesis. Analysis of the repertoire of rearranged immunoglobulin genes in the B cells of microdissected follicles revealed clonal amplification, somatic mutation and isotype switching, indicating a local antigen-driven B-cell response. Surprisingly, IgA responses were observed despite the nonmucosal location of the follicles. Taken together, our findings show the existence of lymphoid neogenesis in melanoma and suggest that the presence of functional ectopic lymphoid structures in direct contact with the tumor makes the local development of antimelanoma B- and T-cell responses possible., (©2012 AACR.)
- Published
- 2012
- Full Text
- View/download PDF
25. Two abundant proteasome subtypes that uniquely process some antigens presented by HLA class I molecules.
- Author
-
Guillaume B, Chapiro J, Stroobant V, Colau D, Van Holle B, Parvizi G, Bousquet-Dubouch MP, Théate I, Parmentier N, and Van den Eynde BJ
- Subjects
- Amino Acid Sequence, Animals, Antigens, Neoplasm genetics, Antigens, Neoplasm metabolism, Cell Line, Tumor, Humans, Mice, Mice, Knockout, Molecular Sequence Data, Neoplasm Proteins genetics, Neoplasm Proteins metabolism, Proteasome Endopeptidase Complex chemistry, Proteasome Endopeptidase Complex genetics, Protein Subunits, Recombinant Proteins genetics, Recombinant Proteins immunology, Recombinant Proteins metabolism, Sequence Homology, Amino Acid, Antigen Presentation, Histocompatibility Antigens Class I metabolism, Proteasome Endopeptidase Complex classification, Proteasome Endopeptidase Complex metabolism
- Abstract
Most antigenic peptides presented by MHC class I molecules result from the degradation of intracellular proteins by the proteasome. In lymphoid tissues and cells exposed to IFNγ, the standard proteasome is replaced by the immunoproteasome, in which all of the standard catalytic subunits β1, β2, and β5 are replaced by their inducible counterparts β1i, β2i, and β5i, which have different cleavage specificities. The immunoproteasome thereby shapes the repertoire of antigenic peptides. The existence of additional forms of proteasomes bearing a mixed assortment of standard and inducible catalytic subunits has been suggested. Using a new set of unique subunit-specific antibodies, we have now isolated, quantified, and characterized human proteasomes that are intermediate between the standard proteasome and the immunoproteasome. They contain only one (β5i) or two (β1i and β5i) of the three inducible catalytic subunits of the immunoproteasome. These intermediate proteasomes represent between one-third and one-half of the proteasome content of human liver, colon, small intestine, and kidney. They are also present in human tumor cells and dendritic cells. We identified two tumor antigens of clinical interest that are processed exclusively either by intermediate proteasomes β5i (MAGE-A3(271-279)) or by intermediate proteasomes β1i-β5i (MAGE-A10(254-262)). The existence of these intermediate proteasomes broadens the repertoire of antigens presented to CD8 T cells and implies that the antigens presented by a given cell depend on their proteasome content.
- Published
- 2010
- Full Text
- View/download PDF
26. Anti-IL-17A autovaccination prevents clinical and histological manifestations of experimental autoimmune encephalomyelitis.
- Author
-
Uyttenhove C, Sommereyns C, Théate I, Michiels T, and Van Snick J
- Subjects
- Animals, Cytokines metabolism, Encephalomyelitis, Autoimmune, Experimental metabolism, Encephalomyelitis, Autoimmune, Experimental pathology, Immunotherapy, Mice, Mice, Inbred C57BL, Encephalomyelitis, Autoimmune, Experimental immunology, Encephalomyelitis, Autoimmune, Experimental therapy, Interleukin-17 immunology, Vaccination
- Abstract
Excessive or inappropriate production of IL-17A has been reported in diseases such as rheumatoid arthritis, asthma, and multiple sclerosis. The potential clinical relevance of these correlations was suggested by the protective effects of anti-IL-17A monoclonal antibodies in various mouse disease models. However, the chronic nature of the corresponding human afflictions raises great challenges for Ab-based therapies. An alternative to passive Ab therapy is autovaccination. Covalent association of self-cytokines with foreign proteins has been reported to induce the production of antibodies capable of neutralizing the biological activity of the target cytokine. We recently reported that cross-linking of IL-17A to ovalbumin produced highly immunogenic complexes that induced long-lasting IL-17A-neutralizing antibodies. Vaccinated SJL mice were completely protected against experimental autoimmune encephalomyelitis (EAE) induced by proteolipid protein peptide (PLP 139-151), and a monoclonal anti-IL-17A Ab (MM17F3), derived from C57Bl/6 mice vaccinated against IL-17A-OVA, also prevented disease development. Here we report that this Ab also protects C57Bl/6 mice from myelin oligdendrocyte glycoprotein (MOG)-induced EAE. Histological analysis of brain sections of C57Bl/6 mice treated with MM17F3 showed a complete absence of inflammatory infiltrates and evidence for a marked inhibition of chemokine and cytokine messages in the spinal cord. These results further extend the analytical and therapeutic potential of the autovaccine procedure.
- Published
- 2007
- Full Text
- View/download PDF
27. Positive regulation of deoxycytidine kinase activity by phosphorylation of Ser-74 in B-cell chronic lymphocytic leukaemia lymphocytes.
- Author
-
Smal C, Van Den Neste E, Maerevoet M, Poiré X, Théate I, and Bontemps F
- Subjects
- Antibodies pharmacology, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, Phosphorylation, Phosphoserine immunology, Tumor Cells, Cultured, Deoxycytidine Kinase metabolism, Leukemia, Lymphocytic, Chronic, B-Cell metabolism, Serine metabolism
- Abstract
Deoxycytidine kinase (dCK) activates several antileukaemic nucleoside analogues. We have recently reported that the activity of dCK, overexpressed in HEK 293T cells, correlates with its phosphorylation level on Ser-74. Here, we show that dCK from B-cell chronic lymphocytic leukaemia (B-CLL) lymphocytes can be detected by an anti-phospho-Ser-74 antibody and that interindividual variability in dCK activity is related to its phosphorylation level on Ser-74. Moreover, pharmacological intervention modified Ser-74 phosphorylation, in close parallel with changes in dCK activity. These results suggest that activation of dCK via phosphorylation of Ser-74 might constitute a new therapeutic strategy to enhance activation and efficacy of nucleoside analogues.
- Published
- 2007
- Full Text
- View/download PDF
28. An improved in vitro model of human intestinal follicle-associated epithelium to study nanoparticle transport by M cells.
- Author
-
des Rieux A, Fievez V, Théate I, Mast J, Préat V, and Schneider YJ
- Subjects
- Amiloride analogs & derivatives, Amiloride pharmacology, B-Lymphocytes ultrastructure, Caco-2 Cells, Cell Differentiation, Coculture Techniques, Epithelial Cells drug effects, Epithelial Cells ultrastructure, Humans, Immunohistochemistry, Intestinal Mucosa cytology, Microscopy, Confocal, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Nystatin pharmacology, Peyer's Patches cytology, Polystyrenes metabolism, Reproducibility of Results, Temperature, B-Lymphocytes metabolism, Drug Carriers metabolism, Epithelial Cells metabolism, Intestinal Mucosa metabolism, Nanoparticles, Peyer's Patches metabolism, Pinocytosis drug effects
- Abstract
An alternative in vitro model of human follicle-associated epithelium (FAE) to study nanoparticle transport mechanisms by M cells was developed and characterized. The previous in vitro model of human FAE has been improved by inverting inserts after Caco-2 cell seeding. Raji and M cells were identified only in inverted co-culture cell monolayers by immunohistochemistry, confocal microscopy, and electron microscopy. The M cell conversion rate evaluated by scanning electron microscopy ranged between 15 and 30% of cells. Transport of 200 nm carboxylated polystyrene nanoparticles was higher and more reproducible in the inverted model. Nanoparticle transport was temperature-dependent, not affected by the presence of EGTA or by potassium depletion, but inhibited by EIPA or nystatin, suggesting that it occurs most likely by macropinocytosis. The inverted model appears more physiologic, functional and reproducible than the normally oriented model.
- Published
- 2007
- Full Text
- View/download PDF
29. Increased protein glycation in fructosamine 3-kinase-deficient mice.
- Author
-
Veiga da-Cunha M, Jacquemin P, Delpierre G, Godfraind C, Théate I, Vertommen D, Clotman F, Lemaigre F, Devuyst O, and Van Schaftingen E
- Subjects
- Animals, Chromatography, High Pressure Liquid, Cytosol enzymology, Erythrocytes enzymology, Exons genetics, Female, Gene Targeting, Glycosylation, Lysine analogs & derivatives, Lysine urine, Male, Mice, Mice, Knockout, Peptides metabolism, Phosphorylation, Phosphotransferases (Alcohol Group Acceptor) genetics, Phosphotransferases (Alcohol Group Acceptor) metabolism, Glycation End Products, Advanced metabolism, Hemoglobins metabolism, Phosphotransferases (Alcohol Group Acceptor) deficiency
- Abstract
Amines, including those present on proteins, spontaneously react with glucose to form fructosamines in a reaction known as glycation. In the present paper, we have explored, through a targeted gene inactivation approach, the role of FN3K (fructosamine 3-kinase), an intracellular enzyme that phosphorylates free and protein-bound fructose-epsilon-lysines and which is potentially involved in protein repair. Fn3k-/- mice looked healthy and had normal blood glucose and serum fructosamine levels. However, their level of haemoglobin-bound fructosamines was approx. 2.5-fold higher than that of control (Fn3k+/+) or Fn3k+/- mice. Other intracellular proteins were also significantly more glycated in Fn3k-/- mice in erythrocytes (1.8-2.2-fold) and in brain, kidney, liver and skeletal muscle (1.2-1.8-fold), indicating that FN3K removes fructosamines from intracellular proteins in vivo. The urinary excretion of free fructose-epsilon-lysine was 10-20-fold higher in fed mice compared with mice starved for 36 h, and did not differ between fed Fn3k+/+ and Fn3k-/- mice, indicating that food is the main source of urinary fructose-epsilon-lysine in these mice and that FN3K does not participate in the metabolism of food-derived fructose-epsilon-lysine. However, in starved animals, the urinary excretion of fructose-epsilon-lysine was 2.5-fold higher in Fn3k-/- mice compared with Fn3k+/+ or Fn3k+/- mice. Furthermore, a marked increase (5-13-fold) was observed in the concentration of free fructose-epsilon-lysine in tissues of fed Fn3k-/- mice compared with control mice, indicating that FN3K participates in the metabolism of endogenously produced fructose-epsilon-lysine. Taken together, these data indicate that FN3K serves as a protein repair enzyme and also in the metabolism of endogenously produced free fructose-epsilon-lysine.
- Published
- 2006
- Full Text
- View/download PDF
30. Activity and safety of combined rituximab with chlorambucil in patients with mantle cell lymphoma.
- Author
-
Bauwens D, Maerevoet M, Michaux L, Théate I, Hagemeijer A, Stul M, Danse E, Costantini S, Vannuffel P, Straetmans N, Vekemans MC, Deneys V, Ferrant A, and Van Den Neste E
- Subjects
- Adult, Aged, Antibodies, Monoclonal administration & dosage, Antibodies, Monoclonal adverse effects, Antibodies, Monoclonal, Murine-Derived, Antineoplastic Combined Chemotherapy Protocols adverse effects, Chlorambucil administration & dosage, Chlorambucil adverse effects, Disease-Free Survival, Female, Hematologic Diseases chemically induced, Humans, Male, Middle Aged, Remission Induction, Rituximab, Treatment Outcome, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Lymphoma, Mantle-Cell drug therapy
- Abstract
We evaluated the combination of rituximab with chlorambucil in patients with mantle cell lymphoma (MCL) not eligible for aggressive therapy. Fourteen patients (male/female: 9/5) were included (two newly diagnosed, 12 relapsed/refractory). The toxicities were neutropenia, thrombopenia and infection. Nine (64%) patients responded; five (36%) achieved complete remission and four (29%) achieved partial remission. The median progression-free survival for responders was 26 months (95% CI, 4-48). Marrow polymerase chain reaction negativity was attained in seven responders. These results suggest that this schedule may have notable antitumour activity in patients with MCL, including patients in relapse after autologous stem cell transplantation.
- Published
- 2005
- Full Text
- View/download PDF
31. Contrasting frequencies of antitumor and anti-vaccine T cells in metastases of a melanoma patient vaccinated with a MAGE tumor antigen.
- Author
-
Lurquin C, Lethé B, De Plaen E, Corbière V, Théate I, van Baren N, Coulie PG, and Boon T
- Subjects
- Antigens, Neoplasm therapeutic use, Cancer Vaccines therapeutic use, Humans, Neoplasm Proteins therapeutic use, Antigens, Neoplasm immunology, Cancer Vaccines immunology, Melanoma immunology, Neoplasm Metastasis immunology, Neoplasm Proteins immunology, T-Lymphocytes, Cytotoxic immunology
- Abstract
Melanoma patients have high frequencies of T cells directed against antigens of their tumor. The frequency of these antitumor T cells in the blood is usually well above that of the anti-vaccine T cells observed after vaccination with tumor antigens. In a patient vaccinated with a MAGE-3 antigen presented by HLA-A1, we measured the frequencies of anti-vaccine and antitumor T cells in several metastases to evaluate their respective potential contribution to tumor rejection. The frequency of anti-MAGE-3.A1 T cells was 1.5 x 10(-5) of CD8 T cells in an invaded lymph node, sixfold higher than in the blood. An antitumor cytotoxic T lymphocyte (CTL) recognizing a MAGE-C2 antigen showed a much higher enrichment with a frequency of approximately 10%, 1,000 times higher than its blood frequency. Several other antitumor T clonotypes had frequencies >1%. Similar findings were made on a regressing cutaneous metastasis. Thus, antitumor T cells were approximately 10,000 times more frequent than anti-vaccine T cells inside metastases, representing the majority of T cells present there. This suggests that the anti-vaccine CTLs are not the effectors that kill the bulk of the tumor cells, but that their interaction with the tumor generates conditions enabling the stimulation of large numbers of antitumor CTLs that proceed to destroy the tumor cells. Naive T cells appear to be stimulated in the course of this process as new antitumor clonotypes arise after vaccination.
- Published
- 2005
- Full Text
- View/download PDF
32. [A-leukemic caecal myeloid sarcoma: a difficult diagnosis].
- Author
-
Nollevaux MC, Delos M, Noël H, Sonet A, Rosière A, and Théate I
- Subjects
- Abdominal Pain, Antigens, CD analysis, CD79 Antigens, Cecal Neoplasms pathology, Diagnosis, Differential, Female, Humans, Immunohistochemistry, Leukemia, Myeloid pathology, Middle Aged, Muramidase analysis, Peroxidase analysis, Receptors, Antigen, B-Cell analysis, Weight Loss, Cecal Neoplasms diagnosis, Leukemia, Myeloid diagnosis
- Abstract
Myeloid sarcoma is a malignant neoplasia composed of abnormal myeloid or monocytic cells, often localized in bones, but also rarely in extra-medullary sites such as lymph nodes, skin and soft tissue. We report a case of caecal myeloid sarcoma, diagnosed in a 60 year old woman who complained from abdominal pain and weight loss, in absence of any medullary disorder. Initially misdiagnosed as a B lymphoma because of a weak positivity for CD79a, the diagnosis of primitive caecal myeloid sarcoma was eventually established after further investigations showing a positivity for lysozyme and myeloperoxidase. This report of such a rare clinical and pathological presentation of a myeloid sarcoma underlines a difficult differential diagnosis for which adequate immunohistochemistry, including lysozyme and myeloperoxydase is mandatory.
- Published
- 2004
- Full Text
- View/download PDF
33. Evidence for a tumoral immune resistance mechanism based on tryptophan degradation by indoleamine 2,3-dioxygenase.
- Author
-
Uyttenhove C, Pilotte L, Théate I, Stroobant V, Colau D, Parmentier N, Boon T, and Van den Eynde BJ
- Subjects
- Animals, Cell Line, Tumor, Female, Humans, Indoleamine-Pyrrole 2,3,-Dioxygenase, Mice, Neoplasm Transplantation, Neoplasms immunology, Neoplasms pathology, Placenta enzymology, Pregnancy, RNA, Messenger metabolism, Tryptophan pharmacology, Tryptophan Oxygenase antagonists & inhibitors, Tryptophan Oxygenase genetics, CD8-Positive T-Lymphocytes immunology, Neoplasms metabolism, Tryptophan analogs & derivatives, Tryptophan metabolism, Tryptophan Oxygenase metabolism, Tumor Escape
- Abstract
T lymphocytes undergo proliferation arrest when exposed to tryptophan shortage, which can be provoked by indoleamine 2,3-dioxygenase (IDO), an enzyme that is expressed in placenta and catalyzes tryptophan degradation. Here we show that most human tumors constitutively express IDO. We also observed that expression of IDO by immunogenic mouse tumor cells prevents their rejection by preimmunized mice. This effect is accompanied by a lack of accumulation of specific T cells at the tumor site and can be partly reverted by systemic treatment of mice with an inhibitor of IDO, in the absence of noticeable toxicity. These results suggest that the efficacy of therapeutic vaccination of cancer patients might be improved by concomitant administration of an IDO inhibitor.
- Published
- 2003
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.