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1. De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Author

Tetreau, Guillaume, Sawaya, Michael R, De Zitter, Elke, Andreeva, Elena A, Banneville, Anne-Sophie, Schibrowsky, Natalie A, Coquelle, Nicolas, Brewster, Aaron S, Grünbein, Marie Luise, Kovacs, Gabriela Nass, Hunter, Mark S, Kloos, Marco, Sierra, Raymond G, Schiro, Giorgio, Qiao, Pei, Stricker, Myriam, Bideshi, Dennis, Young, Iris D, Zala, Ninon, Engilberge, Sylvain, Gorel, Alexander, Signor, Luca, Teulon, Jean-Marie, Hilpert, Mario, Foucar, Lutz, Bielecki, Johan, Bean, Richard, de Wijn, Raphael, Sato, Tokushi, Kirkwood, Henry, Letrun, Romain, Batyuk, Alexander, Snigireva, Irina, Fenel, Daphna, Schubert, Robin, Canfield, Ethan J, Alba, Mario M, Laporte, Frédéric, Després, Laurence, Bacia, Maria, Roux, Amandine, Chapelle, Christian, Riobé, François, Maury, Olivier, Ling, Wai Li, Boutet, Sébastien, Mancuso, Adrian, Gutsche, Irina, Girard, Eric, Barends, Thomas RM, Pellequer, Jean-Luc, Park, Hyun-Woo, Laganowsky, Arthur D, Rodriguez, Jose, Burghammer, Manfred, Shoeman, Robert L, Doak, R Bruce, Weik, Martin, Sauter, Nicholas K, Federici, Brian, Cascio, Duilio, Schlichting, Ilme, and Colletier, Jacques-Philippe

Subjects
Inorganic Chemistry, Biochemistry and Cell Biology, Chemical Sciences, Biological Sciences, Animals, Bacillus thuringiensis, Bacterial Proteins, Endotoxins, Hemolysin Proteins, Larva, Mosquito Control, Nanoparticles
Abstract

Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.

Published
2022

2. De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Author

Tetreau, Guillaume, Sawaya, Michael R, De Zitter, Elke, Andreeva, Elena A, Banneville, Anne-Sophie, Schibrowsky, Natalie, Coquelle, Nicolas, Brewster, Aaron S, Grünbein, Marie Luise, Kovacs, Gabriela Nass, Hunter, Mark S, Kloos, Marco, Sierra, Raymond G, Schiro, Giorgio, Qiao, Pei, Stricker, Myriam, Bideshi, Dennis, Young, Iris D, Zala, Ninon, Engilberge, Sylvain, Gorel, Alexander, Signor, Luca, Teulon, Jean-Marie, Hilpert, Mario, Foucar, Lutz, Bielecki, Johan, Bean, Richard, de Wijn, Raphael, Sato, Tokushi, Kirkwood, Henry, Letrun, Romain, Batyuk, Alexander, Snigireva, Irina, Fenel, Daphna, Schubert, Robin, Canfield, Ethan J, Alba, Mario M, Laporte, Frédéric, Després, Laurence, Bacia, Maria, Roux, Amandine, Chapelle, Christian, Riobé, François, Maury, Olivier, Ling, Wai Li, Boutet, Sébastien, Mancuso, Adrian, Gutsche, Irina, Girard, Eric, Barends, Thomas RM, Pellequer, Jean-Luc, Park, Hyun-Woo, Laganowsky, Arthur D, Rodriguez, Jose, Burghammer, Manfred, Shoeman, Robert L, Doak, R Bruce, Weik, Martin, Sauter, Nicholas K, Federici, Brian, Cascio, Duilio, Schlichting, Ilme, and Colletier, Jacques-Philippe

Subjects
Inorganic Chemistry, Biochemistry and Cell Biology, Chemical Sciences, Biological Sciences
Abstract

Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan , respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo -grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.

Published
2021

3. Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade.

Author

Tetreau, Guillaume, Banneville, Anne-Sophie, Andreeva, Elena A, Brewster, Aaron S, Hunter, Mark S, Sierra, Raymond G, Teulon, Jean-Marie, Young, Iris D, Burke, Niamh, Grünewald, Tilman A, Beaudouin, Joël, Snigireva, Irina, Fernandez-Luna, Maria Teresa, Burt, Alister, Park, Hyun-Woo, Signor, Luca, Bafna, Jayesh A, Sadir, Rabia, Fenel, Daphna, Boeri-Erba, Elisabetta, Bacia, Maria, Zala, Ninon, Laporte, Frédéric, Després, Laurence, Weik, Martin, Boutet, Sébastien, Rosenthal, Martin, Coquelle, Nicolas, Burghammer, Manfred, Cascio, Duilio, Sawaya, Michael R, Winterhalter, Mathias, Gratton, Enrico, Gutsche, Irina, Federici, Brian, Pellequer, Jean-Luc, Sauter, Nicholas K, and Colletier, Jacques-Philippe

Subjects
NIH 3T3 Cells, Cell Membrane, Animals, Humans, Mice, Disulfides, Bacterial Proteins, Endotoxins, Insecticides, Microscopy, Atomic Force, Crystallography, X-Ray, Protein Conformation, Hydrogen-Ion Concentration, Hemolysin Proteins, HEK293 Cells, Sf9 Cells, Microscopy, Atomic Force, Crystallography, X-Ray
Abstract

Cyt1Aa is the one of four crystalline protoxins produced by mosquitocidal bacterium Bacillus thuringiensis israelensis (Bti) that has been shown to delay the evolution of insect resistance in the field. Limiting our understanding of Bti efficacy and the path to improved toxicity and spectrum has been ignorance of how Cyt1Aa crystallizes in vivo and of its mechanism of toxicity. Here, we use serial femtosecond crystallography to determine the Cyt1Aa protoxin structure from sub-micron-sized crystals produced in Bti. Structures determined under various pH/redox conditions illuminate the role played by previously uncharacterized disulfide-bridge and domain-swapped interfaces from crystal formation in Bti to dissolution in the larval mosquito midgut. Biochemical, toxicological and biophysical methods enable the deconvolution of key steps in the Cyt1Aa bioactivation cascade. We additionally show that the size, shape, production yield, pH sensitivity and toxicity of Cyt1Aa crystals grown in Bti can be controlled by single atom substitution.

Published
2020

7. Low phosphate activates STOP1-ALMT1 to rapidly inhibit root cell elongation.

Author

Balzergue, Coline, Dartevelle, Thibault, Godon, Christian, Laugier, Edith, Meisrimler, Claudia, Teulon, Jean-Marie, Creff, Audrey, Bissler, Marie, Brouchoud, Corinne, Hagège, Agnès, Müller, Jens, Chiarenza, Serge, Javot, Hélène, Becuwe-Linka, Noëlle, David, Pascale, Péret, Benjamin, Delannoy, Etienne, Thibaud, Marie-Christine, Armengaud, Jean, Abel, Steffen, Pellequer, Jean-Luc, Nussaume, Laurent, and Desnos, Thierry

Subjects
Arabidopsis, Arabidopsis Proteins, Cell Enlargement, Cell Wall, Gene Expression Regulation, Plant, Iron, Malates, Meristem, Organic Anion Transporters, Oxidoreductases, Peroxidase, Phosphates, Plant Roots, Plants, Genetically Modified, Signal Transduction, Transcription Factors
Abstract

Environmental cues profoundly modulate cell proliferation and cell elongation to inform and direct plant growth and development. External phosphate (Pi) limitation inhibits primary root growth in many plant species. However, the underlying Pi sensory mechanisms are unknown. Here we genetically uncouple two Pi sensing pathways in the root apex of Arabidopsis thaliana. First, the rapid inhibition of cell elongation in the transition zone is controlled by transcription factor STOP1, by its direct target, ALMT1, encoding a malate channel, and by ferroxidase LPR1, which together mediate Fe and peroxidase-dependent cell wall stiffening. Second, during the subsequent slow inhibition of cell proliferation in the apical meristem, which is mediated by LPR1-dependent, but largely STOP1-ALMT1-independent, Fe and callose accumulate in the stem cell niche, leading to meristem reduction. Our work uncovers STOP1 and ALMT1 as a signalling pathway of low Pi availability and exuded malate as an unexpected apoplastic inhibitor of root cell wall expansion.

Published
2017

11. Cryo-EM structure of influenza helical nucleocapsid reveals NP-NP and NP-RNA interactions as a model for the genome encapsidation

Author

Chenavier, Florian, primary, Estrozi, Leandro F., additional, Teulon, Jean-Marie, additional, Zarkadas, Eleftherios, additional, Freslon, Lily-Lorette, additional, Pellequer, Jean-Luc, additional, Ruigrok, Rob W. H., additional, Schoehn, Guy, additional, Ballandras-Colas, Allison, additional, and Crépin, Thibaut, additional

Published
2023
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12. Externalized histone H4 orchestrates chronic inflammation by inducing lytic cell death

Author

Silvestre-Roig, Carlos, Braster, Quinte, Wichapong, Kanin, Lee, Ernest Y., Teulon, Jean Marie, Berrebeh, Nihel, Winter, Janine, Adrover, José M., Santos, Giancarlo Santiago, Froese, Alexander, Lemnitzer, Patricia, Ortega-Gómez, Almudena, Chevre, Raphael, Marschner, Julian, Schumski, Ariane, Winter, Carla, Perez-Olivares, Laura, Pan, Chang, Paulin, Nicole, Schoufour, Tom, Hartwig, Helene, González-Ramos, Silvia, Kamp, Frits, Megens, Remco T. A., Mowen, Kerri A., Gunzer, Matthias, Maegdefessel, Lars, Hackeng, Tilman, Lutgens, Esther, Daemen, Mat, von Blume, Julia, Anders, Hans-Joachim, Nikolaev, Viacheslav O., Pellequer, Jean-Luc, Weber, Christian, Hidalgo, Andrés, Nicolaes, Gerry A. F., Wong, Gerard C. L., and Soehnlein, Oliver

Published
2019
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16. Sequencing intact membrane proteins using MALDI mass spectrometry

Author

Zhamungui Sánchez, Edison, primary, Hijazi, Hassan, additional, Haidar, Jana, additional, Mecarelli, Enrica, additional, Bauda, Elda, additional, Petit-Härtlein, Isabelle, additional, Teulon, Jean-Marie, additional, Pellequer, Jean-Luc, additional, and Boeri Erba, Elisabetta, additional

Published
2023
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18. Correlation between plant cell wall stiffening and root extension arrest phenotype in the combined abiotic stress of Fe and Al.

Author

Kaur, Harinderbir, Teulon, Jean‐Marie, Godon, Christian, Desnos, Thierry, Chen, Shu‐wen W., and Pellequer, Jean‐Luc

Subjects
*PLANT cell walls, *ABIOTIC stress, *ATOMIC force microscopy, *ARREST, *ARABIDOPSIS thaliana
Abstract

The plasticity and growth of plant cell walls (CWs) remain poorly understood at the molecular level. In this work, we used atomic force microscopy (AFM) to observe elastic responses of the root transition zone of 4‐day‐old Arabidopsis thaliana wild‐type and almt1‐mutant seedlings grown under Fe or Al stresses. Elastic parameters were deduced from force‐distance curve measurements using the trimechanic‐3PCS framework. The presence of single metal species Fe2+ or Al3+ at 10 µM exerts no noticeable effect on the root growth compared with the control conditions. On the contrary, a mix of both the metal ions produced a strong root‐extension arrest concomitant with significant increase of CW stiffness. Raising the concentration of either Fe2+ or Al3+ to 20 µM, no root‐extension arrest was observed; nevertheless, an increase in root stiffness occurred. In the presence of both the metal ions at 10 µM, root‐extension arrest was not observed in the almt1 mutant, which substantially abolishes the ability to exude malate. Our results indicate that the combination of Fe2+ and Al3+ with exuded malate is crucial for both CW stiffening and root‐extension arrest. However, stiffness increase induced by single Fe2+ or Al3+ is not sufficient for arresting root growth in our experimental conditions. Summary statement: We investigate the change in stiffness of the external primary cell wall of living Arabidopsis thaliana seedlings in the presence of metallic stress using atomic force microscopy. Results reveal for the first time the uncoupling between mechanical response (CW stiffening) and root extension arrest. [ABSTRACT FROM AUTHOR]

Published
2024
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19. Stiffening of the Plant Root Cell Wall Induced by a Metallic Stress

Author

Godon, Christian, Kaur, Harinderbir, Teulon, Jean-Marie, Chen, Shu-Wen W, Desnos, Thierry, Pellequer, Jean-Luc, Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Groupe Microscopie Electronique et Méthodes (IBS-MEM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), CEA (APTTOX021401 and APTTOX021403), Investissements d’avenir (DEMETERRES), Malgorzata Lekka, Daniel Navajas, Manfred Radmacher, and Alessandro Podestà

Subjects
[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph]
Abstract

International audience

Published
2023
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20. Preparation and Deposition of Plant Roots for AFM Nanomechanical Measurements

Author

Kaur, Harinderbir, Godon, Christian, Teulon, Jean-Marie, Desnos, Thierry, Pellequer, Jean-Luc, Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Groupe Microscopie Electronique et Méthodes (IBS-MEM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), CEA (APTTOX021401, APTTOX021403), Investissements d’avenir (DEMETERRES), Malgorzata Lekka, Daniel Navajas, Manfred Radmacher, and Alessandro Podestà

Subjects
[SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics
Abstract

International audience; In general, cell growth is a mechanical process that balances internal and externalstresses allowing or limiting expansion. Plant cells are often compared to“hydraulicmachines”due to the similar concept of balanced counterforces between the primarywall stresses and the turgor pressure. Knowledge of the mechanics of plant root cellsis essential to understand how plant wall works and, therefore, how plants grow. Onemethod for qualitative and quantitative analysis of mechanical properties is atomicforce microscopy (AFM) that measures mechanical properties of living cells or tissuesunder conditions close to relevant physiological environments (Arnould et al., 2017,Kozlova et al., 2019, Milani et al., 2011, 2014, Peaucelle et al., 2012, 2011, Torode et al.,2018, Yakubov et al., 2016, Zdunek and Kurenda, 2013, Zhao et al., 2005). In our recentstudy, AFM has been used to investigate properties of root epidermal cells (Balzergueet al., 2017), which are the cells that form the outermost layer of the root. These epi-dermal cells were studied in situ on living seedlings and were therefore still influ-enced by the inherent multicellular properties of the root, which is an improvementover studies conducted on isolated living cells.This chapter explains and illustrates the different steps required to prepare anddeposit the seedling, and in particular the root tip, of the plant on a glass slideready for nanomechanical measurements by AFM

Published
2023
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24. Structural and functional characterization of DdrC, a novel DNA damage-induced nucleoid associated protein involved in DNA compaction

Author

Banneville, Anne-Sophie, primary, Bouthier de la Tour, Claire, additional, De Bonis, Salvatore, additional, Hognon, Cécilia, additional, Colletier, Jacques-Philippe, additional, Teulon, Jean-Marie, additional, Le Roy, Aline, additional, Pellequer, Jean-Luc, additional, Monari, Antonio, additional, Dehez, François, additional, Confalonieri, Fabrice, additional, Servant, Pascale, additional, and Timmins, Joanna, additional

Published
2022
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27. De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Author

Tetreau, Guillaume, primary, Sawaya, Michael R., additional, De Zitter, Elke, additional, Andreeva, Elena A., additional, Banneville, Anne-Sophie, additional, Schibrowsky, Natalie, additional, Coquelle, Nicolas, additional, Brewster, Aaron S., additional, Grünbein, Marie Luise, additional, Kovacs, Gabriela Nass, additional, Hunter, Mark S., additional, Kloos, Marco, additional, Sierra, Raymond G., additional, Schiro, Giorgio, additional, Qiao, Pei, additional, Stricker, Myriam, additional, Bideshi, Dennis, additional, Young, Iris D., additional, Zala, Ninon, additional, Engilberge, Sylvain, additional, Gorel, Alexander, additional, Signor, Luca, additional, Teulon, Jean-Marie, additional, Hilpert, Mario, additional, Foucar, Lutz, additional, Bielecki, Johan, additional, Bean, Richard, additional, de Wijn, Raphael, additional, Sato, Tokushi, additional, Kirkwood, Henry, additional, Letrun, Romain, additional, Batyuk, Alexander, additional, Snigireva, Irina, additional, Fenel, Daphna, additional, Schubert, Robin, additional, Canfield, Ethan J., additional, Alba, Mario M., additional, Laporte, Frédéric, additional, Després, Laurence, additional, Bacia, Maria, additional, Roux, Amandine, additional, Chapelle, Christian, additional, Riobé, François, additional, Maury, Olivier, additional, Ling, Wai Li, additional, Boutet, Sébastien, additional, Mancuso, Adrian, additional, Gutsche, Irina, additional, Girard, Eric, additional, Barends, Thomas R. M., additional, Pellequer, Jean-Luc, additional, Park, Hyun-Woo, additional, Laganowsky, Arthur D., additional, Rodriguez, Jose, additional, Burghammer, Manfred, additional, Shoeman, Robert L., additional, Doak, R. Bruce, additional, Weik, Martin, additional, Sauter, Nicholas K., additional, Federici, Brian, additional, Cascio, Duilio, additional, Schlichting, Ilme, additional, and Colletier, Jacques-Philippe, additional

Published
2021
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30. Structural and functional characterization of DdrC, a novel DNA damage-induced nucleoid associated protein involved in DNA compaction

Author

Banneville, Anne-Sophie, primary, de la Tour, Claire Bouthier, additional, Hognon, Cécilia, additional, Colletier, Jacques-Philippe, additional, Teulon, Jean-Marie, additional, Le Roy, Aline, additional, Pellequer, Jean-Luc, additional, Monari, Antonio, additional, Dehez, François, additional, Confalonieri, Fabrice, additional, Servant, Pascale, additional, and Timmins, Joanna, additional

Published
2021
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33. The solid state of anti-inflammatory morniflumate diniflumate: A cocrystalline salt

Author

Universitat Politècnica de Catalunya. Departament de Física, Universitat Politècnica de Catalunya. GCM - Grup de Caracterització de Materials, Barrio Casado, María del, Céolin, René, Robert, Benoit, Allouchi, Hassan, Teulon, Jean-Marie, Guéchot, Christophe, Tamarit Mur, José Luis, Rietveld, Ivo B., Universitat Politècnica de Catalunya. Departament de Física, Universitat Politècnica de Catalunya. GCM - Grup de Caracterització de Materials, Barrio Casado, María del, Céolin, René, Robert, Benoit, Allouchi, Hassan, Teulon, Jean-Marie, Guéchot, Christophe, Tamarit Mur, José Luis, and Rietveld, Ivo B.

Abstract

Morniflumate diniflumate, a molecular compound involving niflumic acid and its ß-morpholino ethyl ester (morniflumate) in the mole ratio 2:1, is found to crystallize in a triclinic P - 1 space group with a unit-cell volume of 2203.4(5) Å3. It is a cocrystal between a morniflumate+ niflumate- salt and a neutral niflumic acid molecule. The co-crystalline salt forms endothermically with a positive excess volume and it melts incongruently at 382.3(8) K. Differential scanning calorimetry executed at heating rates above 20 K·min-1, leads to congruent melting at 387.8(9)K with an enthalpy change of ¿fusH = 80(2) J g-1. The rare occurrence that incongruent and congruent melting can be observed for the same cocrystal may be due to the conformational versatility of the niflumic acid molecule and its slow conversion between the different conformations due to weak intramolecular hydrogen bonding., Peer Reviewed, Postprint (author's final draft)

Published
2021

36. Intrinsically Disordered Tardigrade Proteins Self‐Assemble into Fibrous Gels in Response to Environmental Stress.

Author

Malki, Anas, Teulon, Jean‐Marie, Camacho‐Zarco, Aldo R., Chen, Shu‐wen W., Adamski, Wiktor, Maurin, Damien, Salvi, Nicola, Pellequer, Jean‐Luc, and Blackledge, Martin

Subjects
*DENATURATION of proteins, *NUCLEAR magnetic resonance spectroscopy, *PHYSIOLOGICAL stress, *PROTEINS
Abstract

Tardigrades are remarkable for their ability to survive harsh stress conditions as diverse as extreme temperature and desiccation. The molecular mechanisms that confer this unusual resistance to physical stress remain unknown. Recently, tardigrade‐unique intrinsically disordered proteins have been shown to play an essential role in tardigrade anhydrobiosis. Here, we characterize the conformational and physical behaviour of CAHS‐8 from Hypsibius exemplaris. NMR spectroscopy reveals that the protein comprises an extended central helical domain flanked by disordered termini. Upon concentration, the protein is shown to successively form oligomers, long fibres, and finally gels constituted of fibres in a strongly temperature‐dependent manner. The helical domain forms the core of the fibrillar structure, with the disordered termini remaining highly dynamic within the gel. Soluble proteins can be encapsulated within cavities in the gel, maintaining their functional form. The ability to reversibly form fibrous gels may be associated with the enhanced protective properties of these proteins. [ABSTRACT FROM AUTHOR]

Published
2022
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38. Structural and Functional Characterization of the Type Three Secretion System (T3SS) Needle of Pseudomonas aeruginosa

Author

Lombardi, Charlotte, primary, Tolchard, James, additional, Bouillot, Stephanie, additional, Signor, Luca, additional, Gebus, Caroline, additional, Liebl, David, additional, Fenel, Daphna, additional, Teulon, Jean-Marie, additional, Brock, Juliane, additional, Habenstein, Birgit, additional, Pellequer, Jean-Luc, additional, Faudry, Eric, additional, Loquet, Antoine, additional, Attrée, Ina, additional, Dessen, Andréa, additional, and Job, Viviana, additional

Published
2019
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40. Nano-structural stiffness measure for soft biomaterials of heterogeneous elasticityElectronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2nh00390b

Author

Chen, Shu-wen W., Teulon, Jean-Marie, Kaur, Harinderbir, Godon, Christian, and Pellequer, Jean-Luc

Abstract

Measuring the structural stiffness aims to reveal the impact of nanostructured components or various physiological circumstances on the elastic response of material to an external indentation. With a pyramidal tip at a nano-scale, we employed the atomic force microscopy (AFM) to indent the surfaces of two compositions of polyacrylamide gels with different softness and seedling roots of Arabidopsis thaliana. We found that the stiffness–depth curve derived from the measured force exhibits a heterogeneous character in elasticity. According to the tendency of stiffness–depth curve, we decomposed the responding force into depth-impact (FC), Hookean (FH) and tip-shape (FS) components, called trimechanic, where FSand its gradient should be offset at the surface or subsurfaces of the indented material. Thereby, trimechnic theory allows us to observe how the three restoring nanomechanics change with varied depth. Their strengths are represented by the respective spring constants (kC, kH, kS) of three parallel-connected spring (3PCS) analogs to differentiate restoring nanomechansims of indented materials. The effective Young's modulus Êand the total stiffness kT(= kH+ kS) globally unambiguously distinguish the softness between the two gel categories. Data fluctuations were observed in the elasticity parameters of individual samples, reflecting nanostructural variations in the gel matrix. Similar tendencies were found in the results from growing plant roots, though the data fluctuations are expectedly much more dramatic. The zone-wise representation of stiffness by the trimechanic-3PCS framework demonstrates a stiffness measure that reflects beneath nanostructures encountered by deepened depth. The trimechanic-3PCS framework can apply any mechanical model of power-law based force–depth relationship and is compatible with thin layer corrections. It provides a new paradigm for analyzing restoring nanomechanics of soft biomaterials in response to indenting forces.

Published
2022
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41. Standardized Nanomechanical Atomic Force Microscopy Procedure (SNAP) for Measuring Soft and Biological Samples

Author

Schillers, Hermann, Rianna, Carmela, Schäpe, Jens, Luque, Tomas, Doschke, Holger, Wälte, Mike, Uriarte, Juan José, Campillo, Noelia, Michanetzis, Georgios P. A., Bobrowska, Justyna, Dumitru, Andra, Herruzo, Elena T., Bovio, Simone, Parot, Pierre, Galluzzi, Massimiliano, Podestà, Alessandro, Puricelli, Luca, Scheuring, Simon, Missirlis, Yannis, Garcia, Ricardo, Odorico, Michael, Teulon, Jean-Marie, Lafont, Frank, Lekka, Malgorzata, Rico, Felix, Rigato, Annafrancesca, Pellequer, Jean-Luc, Oberleithner, Hans, Navajas, Daniel, Radmacher, Manfred, Westfälische Wilhelms-Universität Münster ( WWU ), University of Bremen, University of Barcelona, University of Patras [Patras], Polish Academy of Sciences, Instituto de Ciencia de Materiales de Madrid [Madrid] ( ICMM ), Institut Pasteur de Lille, Réseau International des Instituts Pasteur ( RIIP ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ), Università degli studi di Milano [Milano], Department Radiation Oncology (Weill Cornell Medicine), Weill Medical College of Cornell University [New York], Institut de Chimie Séparative de Marcoule ( ICSM - UMR 5257 ), Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Ecole Nationale Supérieure de Chimie de Montpellier ( ENSCM ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de biologie structurale ( IBS - UMR 5075 ), Université Joseph Fourier - Grenoble 1 ( UJF ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Grenoble Alpes ( UGA ), BIO-AFM-LAB Bio Atomic Force Microscopy Laboratory ( Bio-AFM-Lab ), Aix Marseille Université ( AMU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), ANR-10-EQPX-0004/10-EQPX-0004,Imaginex BioMed,Plateau de microscopie de criblage à haut débit et d'analyse à très haute résolution ( 2010 ), European Project : 340177,EC:FP7:ERC,ERC-2013-ADG,3DNANOMECH ( 2014 ), Westfälische Wilhelms-Universität Münster (WWU), Instituto de Ciencia de Materiales de Madrid (ICMM), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Réseau International des Instituts Pasteur (RIIP), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Università degli Studi di Milano [Milano] (UNIMI), Etude de la Matière en Mode Environnemental (L2ME), Institut de Chimie Séparative de Marcoule (ICSM - UMR 5257), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), BIO-AFM-LAB Bio Atomic Force Microscopy Laboratory (Bio-AFM-Lab), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), ANR-10-EQPX-0004,Imaginex BioMed,Plateau de microscopie de criblage à haut débit et d'analyse à très haute résolution(2010), European Project: 340177,EC:FP7:ERC,ERC-2013-ADG,3DNANOMECH(2014), Universitat de Barcelona, Ministerio de Ciencia e Innovación (España), International AFMBioMed Conferences Association, Università degli Studi di Milano, Ministerio de Economía y Competitividad (España), German Research Foundation, European Research Council, Agence Nationale de la Recherche (France), National Science Centre (Poland), Westfälische Wilhelms-Universität Münster = University of Münster (WWU), University of Patras, Polska Akademia Nauk = Polish Academy of Sciences (PAN), Università degli Studi di Milano = University of Milan (UNIMI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)

Subjects
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Nanotecnologia, Science, technology, industry, and agriculture, Reproducibility of Results, Biomecànica, Hydrogels, Microscòpia de força atòmica, Microscopy, Atomic Force, Article, Madin Darby Canine Kidney Cells, Atomic force microscopy, Dogs, Elastic Modulus, Medicine, Animals, Nanotechnology, Biomechanics, Stress, Mechanical, [ SDV.BBM.BS ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

We present a procedure that allows a reliable determination of the elastic (Young's) modulus of soft samples, including living cells, by atomic force microscopy (AFM). The standardized nanomechanical AFM procedure (SNAP) ensures the precise adjustment of the AFM optical lever system, a prerequisite for all kinds of force spectroscopy methods, to obtain reliable values independent of the instrument, laboratory and operator. Measurements of soft hydrogel samples with a well-defined elastic modulus using different AFMs revealed that the uncertainties in the determination of the deflection sensitivity and subsequently cantilever's spring constant were the main sources of error. SNAP eliminates those errors by calculating the correct deflection sensitivity based on spring constants determined with a vibrometer. The procedure was validated within a large network of European laboratories by measuring the elastic properties of gels and living cells, showing that its application reduces the variability in elastic moduli of hydrogels down to 1%, and increased the consistency of living cells elasticity measurements by a factor of two. The high reproducibility of elasticity measurements provided by SNAP could improve significantly the applicability of cell mechanics as a quantitative marker to discriminate between cell types and conditions., This work was supported by grants to D.N., T.L., N.C., and J.J.U. (Spanish Ministry of Economy and Competitiveness FIS-PI14/00280), M.L. and J.B. (National Science Center NCN DEC-2011/01/M/ST3/00711), H.O. (Deutsche Forschungsgemeinschaft, Koselleck 68/1 and Centre of Excellence, Cells in Motion (CIM), University of Münster, Germany), S.B. and F.L. (ANR-10-EQPX-04-01) and A.D., E.T.H., R.G. (CSD2010-00024, ERC-AdG-340177). A.P. thanks the Dept. of Physics of the University of Milano for financial support under the project “Piano di Sviluppo dell’Ateneo per la Ricerca 2014”, actions: “A. Upgrade of instrumentation”, and “B. Support to young researchers”. We acknowledge the support of the International AFMBioMed Conference Association.

Published
2017
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42. On the Operational Aspects of Measuring Nanoparticle Sizes

Author

Teulon, Jean-Marie, primary, Godon, Christian, additional, Chantalat, Louis, additional, Moriscot, Christine, additional, Cambedouzou, Julien, additional, Odorico, Michael, additional, Ravaux, Johann, additional, Podor, Renaud, additional, Gerdil, Adèle, additional, Habert, Aurélie, additional, Herlin-Boime, Nathalie, additional, Chen, Shu-wen, additional, and Pellequer, Jean-Luc, additional

Published
2018
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43. Atomic force microscope, molecular imaging, and analysis

Author

Chen, Shu-Wen, Teulon, Jean-Marie, Godon, Christian, Pellequer, Jean-Luc, Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Signalisation de l'Adaptation des Végétaux à l'Environnement (SAVE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), and Plant Environmental Physiology and Stress Signaling (PEPSS)

Subjects
[CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016
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44. Low phosphate activates STOP1-ALMT1 to rapidly inhibit root cell elongation

Author

Balzergue, Coline, primary, Dartevelle, Thibault, additional, Godon, Christian, additional, Laugier, Edith, additional, Meisrimler, Claudia, additional, Teulon, Jean-Marie, additional, Creff, Audrey, additional, Bissler, Marie, additional, Brouchoud, Corinne, additional, Hagège, Agnès, additional, Müller, Jens, additional, Chiarenza, Serge, additional, Javot, Hélène, additional, Becuwe-Linka, Noëlle, additional, David, Pascale, additional, Péret, Benjamin, additional, Delannoy, Etienne, additional, Thibaud, Marie-Christine, additional, Armengaud, Jean, additional, Abel, Steffen, additional, Pellequer, Jean-Luc, additional, Nussaume, Laurent, additional, and Desnos, Thierry, additional

Published
2017
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45. Standardized nanomechanical atomic force microscopy procedure (SNAP) for measuring soft and biological samples

Author

Ministerio de Ciencia e Innovación (España), International AFMBioMed Conferences Association, Università degli Studi di Milano, Ministerio de Economía y Competitividad (España), German Research Foundation, European Research Council, Agence Nationale de la Recherche (France), National Science Centre (Poland), Schillers, Hermann, Rianna, Carmela, Schäpe, Jens, Luque, Tomás, Doschke, Holger, Wälte, Mike, Uriarte, Juan José, Campillo, Noelia, Michanetzis, Georgios P. A., Bobrowska, Justyna, Dumitru, Andrea, Herruzo, Elena T., Bovio, Simone, Parot, Pierre, Galluzzi, Massimiliano, Podestà, Alessandro, Puricelli, Luca, Scheuring, Simon, Missirlis, Yannis, García García, Ricardo, Odorico, Michael, Teulon, Jean-Marie, Lafont, Frank, Lekka, Malgorzata, Rico, Felix, Rigato, Annafrancesca, Pellequer, Jean-Luc, Oberleithner, Hans, Navajas, Daniel, Radmacher, Manfred, Ministerio de Ciencia e Innovación (España), International AFMBioMed Conferences Association, Università degli Studi di Milano, Ministerio de Economía y Competitividad (España), German Research Foundation, European Research Council, Agence Nationale de la Recherche (France), National Science Centre (Poland), Schillers, Hermann, Rianna, Carmela, Schäpe, Jens, Luque, Tomás, Doschke, Holger, Wälte, Mike, Uriarte, Juan José, Campillo, Noelia, Michanetzis, Georgios P. A., Bobrowska, Justyna, Dumitru, Andrea, Herruzo, Elena T., Bovio, Simone, Parot, Pierre, Galluzzi, Massimiliano, Podestà, Alessandro, Puricelli, Luca, Scheuring, Simon, Missirlis, Yannis, García García, Ricardo, Odorico, Michael, Teulon, Jean-Marie, Lafont, Frank, Lekka, Malgorzata, Rico, Felix, Rigato, Annafrancesca, Pellequer, Jean-Luc, Oberleithner, Hans, Navajas, Daniel, and Radmacher, Manfred

Abstract

We present a procedure that allows a reliable determination of the elastic (Young's) modulus of soft samples, including living cells, by atomic force microscopy (AFM). The standardized nanomechanical AFM procedure (SNAP) ensures the precise adjustment of the AFM optical lever system, a prerequisite for all kinds of force spectroscopy methods, to obtain reliable values independent of the instrument, laboratory and operator. Measurements of soft hydrogel samples with a well-defined elastic modulus using different AFMs revealed that the uncertainties in the determination of the deflection sensitivity and subsequently cantilever's spring constant were the main sources of error. SNAP eliminates those errors by calculating the correct deflection sensitivity based on spring constants determined with a vibrometer. The procedure was validated within a large network of European laboratories by measuring the elastic properties of gels and living cells, showing that its application reduces the variability in elastic moduli of hydrogels down to 1%, and increased the consistency of living cells elasticity measurements by a factor of two. The high reproducibility of elasticity measurements provided by SNAP could improve significantly the applicability of cell mechanics as a quantitative marker to discriminate between cell types and conditions.

Published
2017

48. Crystal structure and solid-state studies of aged samples of tienoxolol, an API designed against hypertension

Author

Teulon, Jean-Marie, Mahé, Nathalie, Do, Bernard, Nicolaï, Béatrice, Rietveld, Ivo B., Barrio Casado, María del, Tamarit Mur, José Luis, Céolin, René, Ghéchot, Christophe, Universitat Politècnica de Catalunya. Departament de Física i Enginyeria Nuclear, and Universitat Politècnica de Catalunya. GCM - Grup de Caracterització de Materials

Subjects
Ciències de la salut::Medicina::Farmacologia [Àrees temàtiques de la UPC], Hypertension Age factors, Física de l'estat sòlid, Física::Física de l'estat sòlid [Àrees temàtiques de la UPC], Indústria farmacèutica -- Investigació, X-ray diffractometer
Abstract

Aging of drug molecules is generally studied following regulatory procedures, i.e. under forced conditions and for relatively limited storage time; therefore naturally aged samples are rare and provide scientific reference data beyond regulatory considerations. Tienoxolol was studied after 25 years of storage in the dark under ambient conditions. About 86% of the samples still consisted of tienoxolol and the main impurity (13%) was caused by the hydrolysis of the ester moiety. Protection from humidity is therefore important. Other sensitive groups containing nitrogen and sulfur appear to be quite stable with less than 0.8% conversion over 25 years. In addition, the crystal structure has been solved. Tienoxolol orange needles were found to crystallize in the orthorhombic non-centrosymmetric space group Iba2, indicating that the crystal is a racemic compound. The unit cell parameters at room temperature are a = 10.069(5)A˚ , b = 45.831(10)A˚ , and c = 9.822(5) ˚A and the unit cell volume is 4533(3) ˚A3 with Z = 8. © 2011 Elsevier B.V.

Published
2012
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50. Uniaxial negative thermal expansion in crystals of tienoxolol

Author

Universitat Politècnica de Catalunya. Departament de Física i Enginyeria Nuclear, Universitat Politècnica de Catalunya. GCM - Grup de Caracterització de Materials, Nicolaï, Beatrice, Rietveld, Ivo B., Barrio Casado, María del, Mahé, Nathalie, Tamarit Mur, José Luis, Céolin, René, Guéchot, Christophe, Teulon, Jean-Marie, Universitat Politècnica de Catalunya. Departament de Física i Enginyeria Nuclear, Universitat Politècnica de Catalunya. GCM - Grup de Caracterització de Materials, Nicolaï, Beatrice, Rietveld, Ivo B., Barrio Casado, María del, Mahé, Nathalie, Tamarit Mur, José Luis, Céolin, René, Guéchot, Christophe, and Teulon, Jean-Marie

Abstract

The thermal expansion of tienoxolol has been investigated by X-ray powder diffraction up to its melting temperature. The data indicate that the expansion is anisotropic and even negative in one direction of the unit cell. The supramolecular structure formed by hydrogenbonds reflects that of a trellis, which explains the observed behavior of tienoxolol crystals., Postprint (published version)

Published
2012

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